AU606792B2

AU606792B2 - Novel acetylenic, cyano and allenic aristeromycin/adenosine derivatives

Info

Publication number: AU606792B2
Application number: AU31737/89A
Authority: AU
Inventors: Michael L. Edwards; James R. Mccarthy; Nellikunja J. Prakash
Original assignee: Merrell Dow Pharmaceuticals Inc
Current assignee: Aventis Pharmaceuticals Inc
Priority date: 1988-03-25
Filing date: 1989-03-28
Publication date: 1991-02-14
Anticipated expiration: 2009-03-28
Also published as: KR0135529B1; ZA892105B; US4996308A; NZ228422A; DE68922235T2; EP0334361A3; DK145289D0; NO171856C; FI891339A; DK145289A; JPH01283285A; CN1023804C; DK171645B1; ES2074060T3; FI891339A0; NO171856B; IE890953L; JP2829737B2; AR247403A1; CN1036579A

Description

AUSTRALIA

Patents Act COMPLETE SPECIFICATION

(ORIGINAL)

0 nt. Class 9 Int. Class Class Application Number: Lodged: Complete Specification Lodged: Accepted: Published: Priority dl-'U;Ti -at c( a ins an;? 9dc:i's rndi d i ni 5, j Se,',21 o'i 49 di,,l is cor;<Lct j Frzgz'ti;: Related Art: APPLICANT'S REFERENCE: Y1295 AU Name(s) of Applicant(s): Merrell Dow Pharmaceuticals Inc Address(es) of Applicant(s): 2110 East Galbraith Road, Cincinnati, Ohio, UNITED STATES OF AMERICA.

Address for Service is: PHILLIPS ORMCtDE FITZPATRICK Patent and Trade Mark Attorneys 367 Collins Street Melbourne 3000 AUSTRALIA Complete Specification for the invention entitled: NOVEL ACETLMIC, CYXANO AND ALLENIC ARISTEROMYCIN/DEMOSINE DERIVATIVES Our Ref 125810 POF Code: 1432/1432 The following statement is a full description of this invention, including the best method of performing it knovn to applicant(s): 6003q/1 1 NOVEL ACETYLENIC, CYANO AND ALLENIC ARISTEROMYCIN/ADENOSINE DERIVATIVES BACKGROUND OF THE INVENTION i

I

I j 1 te S I o 4t

SI,

I

S-Adenosyl-L-methionine (AdoMet) dependent transmethylation reactions have been implicated in a variety of biological processes related to viral growth and replication, viral transformation of cells, growth of 5 malignant cells, and processes such as chemotaxis and secretion (See P. M. Ueland, Pharm. Reviews, 34, 223 (1982)]. In general, these transmethylation reactions are catalyzed by various transmethylases which utilize AdoMet as a methyl-donor substrate in the methylation of a number 10 of methyl-acceptor substrates such as catechols; norepinephrine; histamine; serotonin; tryptamine; membrane phospholipids; lysyl, arginyl, histidyl, aspartyl, glutamyl, and carboxyl groups of certain proteins; tRNA and mRNA; and DNA. These various transmethylases produce S-Adenosine-L-Homocysteine (AdoHcy) as a byproduct upon transfer of a methyl group from AdoMet to the appropriate methyl-acceptor substrate.

AdoHcy has been shown to be a potent feed-back inhibitor of the AdoMet-dependent transmethylation reactions. This feed-back inhibition of the transmethylases is controlled by the biodegradation of M01295 -1A- AdoHcy by S-Adenosyl-L-Homocysteine Hydrolase which provides a homeostatic control on the tissue levels of AdoHcy. The activity of S-Adenosyl-L-Homocysteine Hydrolase is generally coLisidered by those skilled in the art to play an important role in regulating the tissue levels of AdoHcy and thereby controlling the activity of the AdoMet dependent transmethylation reactions.

The compounds of the present invention are inhibitors of S-Adenosyl-L-Homocysteine Hydrolase. These compounds therefore inhibit the naturally-occurring biodegradation of AdoHcy and result in elevated tissue levels of AdoHcy.

Elevated levels of AdoHcy in turn provide an endogenous I feed-back inhibition of various AdoMet dependent SS, transmethylation reactions which are associated with 15 biological processes related to viral growth and 8 8 replication, viral transformation of cells, growth of o 0 8 o malignant cells, and processes such as chemotaxis and secretion. The compounds of the present invention are therefore useful as inhibitors of these biological S 20 processes and useful in an end use application as therapeutic agents in the treatment of patients afflicted with various pathological conditions in which these processes are implicated, such as, viral infections and neoplastic disease states.

M01295 -2- SUMMARY OF THE INVENTION The present invention relates to novel acetylenic, cyano and allenic aristeromycin/adenosine derivatives which are useful as inhibitors of S-Adenosyl-L- Homocysteine Hydrolase and are useful as anti-viral and anti-neoplastic agents.

The present invention provides novel compounds of the formula (1)

Q

0 t 0 4 4 H0 0A H A l .OH A2 S"o wherein 0 V is oxy, methylene, or thio, R0 is ethynyl or cyano, Ai and A2 are each independently hydrogen, halogen, I or hydroxy with the provisos that where A1 is hydroxy, H Al S' A2 is hydrogen, and that where A2 is hydroxy, AI is H

H

hydrogen, YI is nitrogen, a CH group, a CC1 group, a CBr group or a CNHz group, Y2 and Y3 are each independently nitrogen or a CH group, Q is oNH, NHOR, NHCH3, or hydrogen, and Z is hydrogen, halogen, or NH2; and pharmaceutically-acceptable salts thereof.

M01295 -3- M01295 -3-

V

In addition, the present invention also provides novel compounds of the formula (la)

Q

N

yl2 Y1 V N R2HC C Y3 OH A 2 l la) ~(la) wherein V is oxy, methylene, or thio,

R

2 is hydrogen or C 1

-C

4 alkyl, Al and A 2 are each independently hydrogen, halogen, or hydroxy with the provisos that where Al is hydroxy,

A

2 is hydrogen, and that where A 2 is hydroxy, A 1 is 10 hydrogen,

Y

1 is nitrogen, a CH group, a CC1 group, a CBr group or a

CNH

2 group,

Y

2 and Y 3 are each independently nitrogen or a CH group, Q is NH 2 NHOH, NHCH 3 or hydrogen, and Z is hydrogen, halogen, or NH 2 and pharmaceutically-acceptable salts thereof.

M01295 -4- The present invention also provides a method of inhibiting AdoMet-dependent transmethylation activity in a patient in need thereof comprising administration of an effective inhibitory amount of a compound of formula (1) or (la).

Another embodiment of the present invention is a method of treating a patient afflicted with a neoplastic disease state or in controlling the growth of a neoplasm in a patient afflicted with a neoplastic disease state comprising administration of an effective antineoplastic dose of a compound of formula or (la).

A further embodiment of the present invention is a method of treating a patient afflicted with a viral infection or of controlling a viral infection in a patient S 15 afflicted therewith comprising administration of an effective antiviral amount of a compound of formula or (la).

DETAILED DESCRIPTION OF THE INVENTION As used herein, the term "halogen" refers to a fluorine, chlorine, bromine, or iodine atom and the term "nitrogen" refers to a trivalent nitrogen atom attached to two radicals. The term "ethynyl" refers to a radical of the formula -CsCH and the term "cyano" refers to a radical of the formula -CsN. The term "CI-C 4 alkyl" refers to a saturated straight or branched chain hydrocarbyl radical of one to four carbon atoms.

The aristeromycin/adenosine derivatives of formula (1) or (la) can be prepared by utilizing procedures and techniques well known and appreciated by one skilled in the art.

M01295 1 i-ii- -L daa-n~n~c~ar~ ~Lri~l82ri~- -e A general synthetic procedure for the preparation of compounds of formula wherein RI is ethynyl is set forth in Scheme A. In the following schemes all substituents, unless otherwise indicated, are as previously defined.

SCHEME A Y2 Y3 Z

HO

step a step a "Y2 Y3 OH A2 OB A2B N Y2

Y

3 step b step b step c step c 0 B A 2

B

M01295 -6- .1 Scheme A (cont'd) 7l

Y

3 08 A2B H C step d

Y'<

N H

H

OB A2B 9 4tt4~9 4* 44 4 4, *4 4 4 step e HC C

NY

2

Y

3 step f OB A2B (7) M01295-7

I

Scheme A (cont'd)

N

1 I 2 HC C V A Y3 Z

H

OH A2 (8 gruIn step a, reactive hydroxy, amino, or hydroxylamino S groups other than the 5'-hydroxy group of the appropriate Sf. starting material, as represented by formula are blocked with standard blocking agents well known in the art. These blocking groups can be conventional amino protecting groups for Q and Z (wherein Q or Z are NH 2 and 0 conventional hydroxy protecting groups for the 3'-hydroxy, for A 1 or A 2 (wherein A 1 or A 2 are OH), and for Q (wherein Q is hydroxylamino). OB, A 1 B, A 2 B, QB and ZB in Scheme A represent the 3'-hydroxy, Aj, A 2 Q, and Z groups as herein defined blocked with a blocking group where appropriate.

The selection and utilization of particular blocking J groupc are well known to one of ordinary skill in the art.

In general, blocking groups should be selected which J adequately protect the amino or hydroxy groups in question Sduring subsequent synthetic steps and which are readily removable under conditions which will not cause degradation of the desired product.

M01295 -8- Examples of suitable hydroxy protecting groups are C 1

C

6 alkyl, tetrahydropyranyl, methoxymethyl, methoxyethoxymethyl, t-butyl, benzyl, and triphenylmethyl. The term Cl-C6 alkyl refers to a saturated hydrocarbyl radical of one to six carbon atoms of straight, branched, or cyclic configuration. The preferred blocking groups for the 3'hydroxy and for A 2 (wherein A 2 is hydroxy) include isopropylidene (formed by reacting the unblocked compound with acetone) and alkoxymethyldene (formed by reacting the unblocked compound with trialkylorthoformate).

Examples of suitable amino protecting groups are benzoyl, formyl, acetyl, trifluoroacetyl, phthalyl, tosyl, benzenesulfonyl, benzyloxycarbonyl, substitutedbenzyloxycarbonyl p-chloro,p-bromo, p-nitro, pmethoxy, o-chloro, 2,4-dichloro, and 2,6-dichloro derivatives), t-butyloxycarbonyl (Boc), t-amyloxycarbonyl, isopropyloxycarbonyl, 2-(p-biphenyl)-isopropyloxycarbonyl, allyloxycarbonyl, cyclopentyloxycarbonyl, cyclohexyloxycarbonyl, adamantyloxycarbonyl, phenylthiocarbonyl, and triphenylmethyl. The preferred amino protecting group is the di-benzoyl derivative made by reacting the unblocked compound with benzoyl chloride.

In step b, the appropriately blocked derivative is oxidized to the corresponding aldehyde The preferred oxidizing reagent is dicyclohexylcarbodiimide, methyl phosphonic or dichloroacetic acid and dimethylsulfoxide.

M01295 -9- The aldehyde can optionally be derivatized so as to improve the handling characteristics of the compound or to facilitate purification thereof by means of procedures and techniques well known and appreciated in the art. For example, the derivative can be prepared by the method of Ranganathan et S al. Org. Chem., 39, 290 (1974)].

i In step c, the vinyl chloride derivative is formed by reacting the corresponding aldehyde with 10 chloromethyltriphenylphosphonium chloride or similar alkylating reagent. Chloromethyltriphenylphospnonium chloride is preferred.

i I In step d, the vinyl chloride derivative is dehydrohalogenated to form the ethynyl derivative The preferred reagent to effect the dehydrohalogenation is S lithium diisopropylamide.

In step e, the amino-protecting groups are removed i utilizing procedures and techniques well known and appreciated in the art. For example, the benzoyl amino blocking groups can be removed by hydrolysis with ammonia.

In step f, the hydroxy protecting groups are removed according to conventional procedures and techniques well known and appreciated in the art. For example, the 0-isopropylidene blocking group can be removed by reacting with aqueous trifluroacetic acid.

I Starting materials for use in the general synthetic procedure outlined in Scheme A are readily available to one of ordinary skill in the art. For example, certain starting materials for various compounds of formula (1) and (la) are listed in Table 1.

*401295

L

TABLE 1 Examples of Starting Materials for Scheme A Compound of formula (1) or (1a) wherein Source of V Al A 2

Y

1

Y

2

Y

3 Z Q Starting Material 0 H OH CH N CH H NH 2 J. Med. Chem. 626(1982) O OH H CH N N H NH 2 Het. Chem. 14, 195 (1977)

CH

2 H OH CH N N H NH 2 JACS 88, 3885 (1966) O H H CH 11 N H NH 2 2'-Deoxyadenosine (commer- _____cially available)

CH

2 H OH CH N CH H NH 2 J. Med. Chem. 626 (1982) O OH H CH N N F NH 2 JACS 86, 1242 (19647- 0 H OH CH CH N H NH 2 Nucleosides Nucleotides, 1985, p. 625

CH

2 H OH CH N N H NH 2 J. Pharm. Sci. 62, 1252 (1973)

CH

2 H CH 2 CH N N NH 2

NH

2 J. Med. Chem. 27, 670 (1984)

CH

2 H H CH N N H NH 2 J. Med. Chem. 27, 1416 (1984)

CH

2 OH H CH N N H NH 2 J. Med. Chem. 612 (1977)

CH

2 H OH N N N H NH 2 J. Het. Chem. 601 (1973)

CH

2 H H N N N NH 2

NH

2 J. Med. Chem. 27, 1416 (1984)

CH

2 H H N N N H NH 2 J. Het. Chem. 601 (1973)

CH

2 H H N. N N NH 2

NH

2 J. Med. Chem. 27, ____1416 (1984)

I

M01295 -11- L. OH A 2 wherein V is oxy, methylene or thio, /2 P 7 TABLE 1 (cont'd) Examples of Starting Materials for Scheme A Compound of formula or (la) wherein Source of V A 1 A2 Y 1

Y

2

Y

3 Z Q Starting Material CH2 H OH N N N NH2 NH 2 J. Med Chem. 27, 670(1904)

CH

2 OH H N N N NH 2

NH

2 J. Pharm. Sci. 69, 1019(1980)

CH

2 H OH CH CH N H NH2 Nucleosides Nucleotides 3, 345(1984)

CH

2 H OH CH CH N H NHCH 3 JACS85,193 (1963T

CH

2 H OH CBr CH N H NH 2 JACS86,1242 (1964T S H OH CH N N H NH2 Biochemistry 9, S2367(1970) Additional starting materials can be prepared by the use of methods analogous to those described in Table 1 as well as other conventional methods as are well known and appreciated in the art.

The following example presents a typical synthesis -s described by Scheme A. This example is understood to L, illustrative only and is not intended to limit the scope of the present invention in any way.

M01295 -12-

,Y.

j -i Y'LUU J Or a

CNH

2 group, Y2 and Y3 are each independently nitrogen or a CH group, Q is NH 2 NHOH, NHCH 3 or hydrogen, and Z is hydrogen, halogen, or NH 2 and pharmaceutically-acceptable salts thereof.

EXAMPLE 1 ,6',6'-Tetradehydro-5',6'-Dideoxy--D-Ribo- Hexofuranosyl)-9-H-Purin-6-Amine Step a: NA-benzoyl-2',3'-0-isopropylidene-adenosine.

Convert adenosine to its 2',3'-acetonide followed by benzoylation to the N 6 -benzoyl derivative according to the procedure of Smrt et al. Coll. Czech. Chem. Comm. 29, 224 (1964)].

Step b: N,NA-Bis benzoyl-5'-deoxy-2',3'-0-isopropylidene- Convert N 6 -benzoyl-2',3'-0-isopropylidene adenosine to

N

6 -benzoyl-5'-deoxy-2',3'-0-isopropylidene-5',5'-(N,N'diphenylethylenediamino)adenosine according to the 1 procedure of Ranganathan et al. Org. Chem. 39, 290 (1974)]. To 2.96 g of this product in 10 ml of pyridine, cooled in an ice bath, add 1.15 ml (9.9 mmol) of benzoyl chloride. Stir the mixture overnight at room temperature and pour into ice water. Extract the product into 100 ml of chloroform and dry with magnesium sulfate. Evaporate the solution on a rotary evaporator and add toluene.

Repeat the evaporation inuacuo, and collect 4.07 g of a yellow foam. Percolate the product through a 40 mm X cm flash silica gel column with 4% ethyl acetate/96% dichloromethane. Combine and evaporate the appropriate fractions and collect a yellow oil. Dissolve the oil in ethanol and evaporate three times to yield a solid.

Triturate the solid with 50 ml of ethanol and filter. Dry the solid invacuo to give 2.67 g of the title compound (mp 135-138 degrees Celsius NMR (CDC1 3 90 MHz): 81.30 (3H, S) 1.50 (3H, 3.3-3.7 (4H, 4.55 (1i, r 5.1 (2H, d, J 5.65 (1H, d, J 6.1 (1H, 6.3-7.8 21H, 8.40 (1H, S).

M01295 -13- ~UUC The following statement is a full description of this invention, including the best method of performing it known to applicant(s): 6003q/1 -1- '4 Step b continued: N&,N -Bisbenzoyl-2',3'-0-isopropylidene To 2.64 g (3.73 mmol) of N 6

,N

6 2',3'-0-isopropylidene-5',5'-(N,N'-diphenylethylenediamino)adenosine in 370 ml of dichloromethane at 0 0 C add a solution of 1.56 g (8.2 mmol) p-toluenesulfonic acid monohydrate in 180 ml of acetone. Stir the mixture for hours and filter. Evaporate the filtrate on a rotary evaporator and partition the residue between 200 ml of dichloromethane and water. Dry the dichloromethane solution with magnesium sulfate and evaporate to a foam.

Dissolve 2.10 g of the foam in 200 ml of benzene and reflux in a Dean-Stark apparatus for one hour. Evaporate i the solvent to give 2.06 g of the title compound. (NMR 15 Spectrum reveals more than 80% of the product as aldehyde.) NMR (CDC1 3 90 MHz): 8 1.40 (3H, 3) 1.70 (3H, 4.65 5.3 (1I, d, J 5.45 (1H, broad d, J 7), 6.2 (1H, 7.2-7.8 (10H, 8.10 (1H, 8.45 (major) 20 and 8.55 (lH together, two 9.3 (1H, S, CHO).

Step c: N6 N6-Bis-benzoyl-2',3'-0-isopropylidene-9-(6'chloro-5',6'-dideoxy-5-D-ribohex-5'-enofuranosyl)-9-Hpurin-6-amine.

To a solution of lithium diisopropylamide (20 mmol) in 500 ml tetrahydrofuran (THF) which has been chilled to add chloromethyltriphenylphosphonium chloride (6.8 g, 20 mmol). Allow the mixture to warm to 0 0 C and hold at this temperature for 1 hr. Chill the solution to -700C and K add in a dropwise manner a solution of the N6,N6-bisbenzoyl-2',3'-0-isopropylideneadenosine-5'-aldehyde g, 13 mmol) in THF (100 ml). Stir the reaction mixture at -700C for 2 hr and thereafter pour the mixture into water/dichloromethane. Separate the organic layer, dry M01295 -14i Inhibitor of the AdoMet-dependent transmethylation reactions. This feed-back inhibition of the transmethylases is controlled by the biodegradation of M01295 -1A- L- -rr~l

I-'

-i~ 1with anhydrous magnesium sulfate, and evaporate to dryness. Chromatograph the residue on a silica gel flash column eluting with hexane/ethyl acetate to yield the title compound (4.1 g) as a white foam.

MS (CI) MHF+ 546 Anal: Calc'd for C 28 24 C1N 5 0 5 *1/3 CH 3

C(O)OC

2

H

5 C 61.14, H 4.68, N 12.17 Found: C 61.35, H 4.62, N 12.25 Step d: NAN& -_Bisbenzoyl-9-(5' ,5',6',6'-tetradehydro- 5',6'-dideoxy-2',3'-0-isopropylidene-3-D-ribohexofuranosyl)-9-H-purin-6-amine *a It

S

To a solution of lithium diisopropylamide (8 mmol) in 120 ml THF which has been chilled to -70 0 C, add in a dropwise manner a solution of N6,N6-Bis-benzoyl-2',3'-0isopropylidene-9-(6-chloro-5,6-dideoxy-o-D-ribohex-5enofuranosyl)-9-H-purin-6-amine in 15 ml of THF. Stir the reaction mixture at -70 0 C for 2 hr. Pour the mixture into water and extract the aqueous mixture with dichloromethane. Combine the organic extracts, dry over anhydrous MgSO 4 and evaporate the organic solvent to dryness. Chromatograph the residue on a silica gel flash column eluting with hexane/ethyl acetate to yield the title compound (0.8 g) as a foam.

MS: (CI) MH+ 510.

Step e: 9-(5',5',6',6'-Tetradehydro-5',6'-dideoxy-2',3'- 0-isopropylidene-0-D-ribo-hexofuranosyl)-9-H-purin-6-amine Stir a mixture of N6,N6- Bisbenzoyl-9-(5',5',6',6'tetradehydro-5',6'-dideoxy-2',3'-0-isopropylidene--Dribo-hexofuranosyl)-9-H-purin-6-amine (800 mg, 1.6 mmol) M01295 i in 10 ml of methanol and 10 ml of concentrated aqueous ammonia overnight at ambient temperature. Evaporate the mixture and chromatograph the residue on a silica gel flash column eluting with ethyl acetate. Recrystallize the purified title compound from hexane/ethyl acetate to yield a white solid (170 mg) with a melting point of 210-211 0

C.

Anal: Calc'd for C 14

H

15

N

5 0 5 C 55.81, H 5.02, N 23.24 Found: C 55.65, H 5.03, N 22.98 MS: (CI) MH+ 302.

10 Step f: 9-(5',5',6',6'-Tetradehydro-5',6'-dideoxy-3-Dribo-hexofuranosyl)-9-H-purin-6-amine Heat a solution of 9-(5',5',6',6'-Tetradehydro-5',6'dideoxy-2',3'-0-isopropylidene--D-ribo-hexofuranosyl)-9- S' H-purin-6-amine (270 mg) in water/formic acid (30 ml each) in a 50 0 C oil bath under a nitrogen atmosphere for 2 hr.

Evaporate the mixture and recrystallize the residue from methanol to yield the title compound (122 mg) as a white solid with a melting point of 213-214 0

C.

Anal: Calc'd for C 1

H

11 N0O 3 3/4 H20: C 48.08, H 4.58, N 25.49 Found: C 48.43, H 4.52, N 25.12 MS: (CI) MH+ 262.

M01295 -16-

I

The following specific compounds can be made by procedures analogous to those described above in, Example 1: ,6'-Tetradehydro-5' ,6'-dideoxy-a-Dribofuranosyl)-3r-l,2,3-triazolo[4,5-d]pyrimidin-7-arrine 1-(5',5',6',6'-Tetradehydro-5',6'-dideoxy-a-Dribofuranosyl) -lH-imidazo[14,5-c] pyridin-4-amine ,6'-Tetradehydro-5' ,6'-dideoxy-4-thio-B-D-ribohexofuranosyl)-9-purin-6-amine 10 ,6'-Tetradehydro-5' ,6'-dideoxy-B-D--arabinohexofuranosyl )-9H--purin-6--amine lR-(l.alpha. ,2.alpha. ,3.beta. ,5.beta. )-3-(9H-purin-6amine-9-yl) -5-ethynyl-l, 2-cyclopentanediol The aristeromycin/adenosine derivatives of the formula wherein RI is cyano can be prepared according to conventional procedures and techniques well known and appreciated by one of ordinary skill in the art. A general synthetic procedure is set forth in Scheme B.

M01295 -7 -17- SCHEME B

H

OB A 2

B

.'y 2 Z)7

Y

3

CH

2 0-

Y

3 step a OB A 2

B

N E--C

Y

3 step b step c 0 H A 2

B

N C

H

N

7 2

Y

3 N z

H

step d Y3 OB 2 (11) OH A2 (12) MO01295 -8 -18-

T__

In step a, the aldehyde derivative in which the appropriate amino and hydroxy groups have been blocked in Sa manner analogous to that described in Scheme A is converted to the corresponding oxime derivative The preferred reagent for this reaction is O-benzylhydroxylamine which results in the O-benzyloxime being formed.

The aldehyde derivative can alternatively be converted to the free oxime by reaction with hydroxylamine hydrochloride.

The oxime derivative is then converted to the cyano derivative (10) in step b by reaction with an appropriate base such as lithium diisopropylamide. Where the free oxime is used, the preferred reagent is tosyl chloride in an appropriate base.

In steps c and d, the amino and hydroxy blocking groups can then be removed in a manner analogous to that Sdescribed in Scheme A (steps e and f).

Alternatively, the aristeromycin/adenosine derivatives of formula wherein Ri is cyano can be prepared by converting the appropriate 5'-acid or corresponding ester [wherein the reactive hydroxy, amino, or hydroxylamino groups are blocked as described in Scheme A (step to the 5'-carboxamide by procedures well known and appreciated in the art. This 5'-carboxamide can then be 25 dehydrated to the desired nitrile by use of a variety of reagents such as, for example, phosphorus pentoxide, phosphoryl chloride/pyridine or trichloromethyl chloroformate. The blocking groups can then be removed as described in Scheme A (steps e and f).

M01295 -19-

I

The following example presents a typical synthesis as described by Scheme B. This example is understood to be illustrative only and is not intended to limit the scope of the present invention in any way.

EXAMPLE 2 9-(5-D-Ribofuranylnitrile)-9-H-Purin-6-Amine Step a: N ,N6-Bisbenzoyl-5'-deoxy-2',3'-O-isopropylidene- Prepare N6,N6-bisbenzoyl-2',3'-0-isopropylidene adenosine-5'-aldehyde as described in Example 1. Combine the adenosine aldehyde derivative (2.6 g, 3.7 mmol) and 0benylhydroxylamine (0.85 g, 4 mmol) in 50 ml ethanol and heat at 60 0 C in an oil bath until the reaction is complete. Evaporate the reaction mixture and chromatograph the residue on a flash silica gel column eluting with ethyl acetate/hexane to yield the title compound as a foam.

Step b: N6 ,N-Bisbenzoyl-9-(2',3'-0-isopropylidene- -Dribofuranylnitrile)-9-H-purin-6-amine Add N6,N6-bisbenzoyl-5'-deoxy-2',3'-0-isopropylidene- (618 mg, 1 mmol) to a solution of lithium diisopropylamide (2 mmol) in THF ml) which is chilled to -70C. Stir the reaction mixture at -70 0 C until the reaction is complete as indicated by thin layer chromatography. Pour the mixture into water/dichloromethane and allow the layers to separate. Dry the organic layer over anhydrous magnesium sulfate and evaporate to dryness. Chromatograph the residue on a flash silica gel column eluting with ethyl acetate/hexane to yield the title compound as a foam.

M01295 Steps c and d: 9-(3-D-Ribofuranylnitrile)-9-H-purin-6amine, Sequentially de-block the N 6

,N

6 -Bisbenzoyl-9-(2' isopropylidene-j-D-ribofuranylnitrile)-9-H-purin-6-amine as described in steps e and f of Example 1 to yield the title compound. Recrystallize the product from methanol.

The following specific compounds can be made by procedures analogous to those described above in Example 2: 8-D-arabinofuranonitrile)-9H-purin-6-amine 9-(4-thio-a-D-ribofuranonitrile)-9H-purin-6-amine lR-(l.alpha., 2.alpha., 3.beta., 5.beta.)-3--(9H-purin-6amine-9-yl )-5-cyano-l, 2-cyclopentanedilol l-(Q-D-ribofuranonitrile)-lH-imidazo[l4,5-clpyridin-4-amine The allenic aristeromycin/adenosine derivatives of the formula (la) can be prepared according to conventional procedures and techniques well known in the aii... A general synthetic procedure for the preparation of compounds of formula (la) is set forth in Scheme C.

M01295 -1 -21i ^-~IT-i.lil-ii ^~LIII Scheme C HC= C N Y2

Y

3

N

Y2

N

R2HC=C A

Y

3

O

B A2

B

(13) step a OB A2B step b-- step b

//N

Y

1 R2HC=C OH A2

Y

3 (14) In step a, the partially de-blocked acetylenic derivative which is prepared according to the procedures outlined in Scheme A and bears blocking groups on any reactive hydroxy moieties, is isomerized with base to provide the desired allenic derivative Where a compound of formula (la) is desired wherein R 2 is hydrogen, the acetylenic derivative can be reacted with sodium ethoxide. Where a compound of formula (la) is desired wherein R 2 is alkyl, the acetylenic derivative (7) can be treated with base such as lithium diisopropylamide, and reacted with the appropriate Cl-C 4 alkyl halide according to procedures well known and appreciated in the art. This results in the formation of the appropriately M01295 -22- JI~*leyi I-c-sl- Ylr o t 01 0 alkylated acetylenic derivative which can then be further reacted as described above in steps and In step b, the amino blocking groups can then be removed in a manner analogous to that described in Scheme A (step f).

The following example presents a typical synthesis as described by Scheme C. This example is understood to be illustrative only and is not intended to limit the scope of the present invention in any way.

EXAMPLE 3 9-(4',5',5',6'-Tetradehydro-5',6'-Dideoxy-P-D-Ribohexofuranosyl)-9-H-Purin-6-Amine Step a: 9-(4',5',5',6'-tetradehydro-5'r6'-dideoxy-2',3'-Oisopropylidene-P-D-ribo-hexofuranosyl)-9-H-purin-6-amine Prepare 9-(5',5',,',6'-tetradehydro-5',6'-dideoxy- 2',3'-0-isopropylidene--D-ribo-hexofuranosyl)-9-H-purin- 6-amine as described in Example 1. Add the partially blocked acetylenic derivative (600 mg) to a solution of sodium ethoxide in ethanol which has been prepared by dissolving 100 mg of sodium metal in 20 ml of ethanol.

Reflux the reaction mixture for 1 hr under a nitrogen atmosphere. Cool the mixture, pour into water/dichloromethane, and allow the organic phase to separate. Dry the organic layer over anhydrous magnesium sulfate and evaporate to dryness. Recrystallize from a mixture of hexane/dichloromethane/methanol to yield the title compound (230 mg) as a white powder with a melting point of 209 0 C (shrinks 182 0

C).

MS (CI) MH+ 302 M01295 -23- Anal: Calc'd for C 1 4

H

1 ,N,0 3 C 55.81, H 5.02, N 23.24 Found: C 55.36, H 5.03, N 23.46 NMR (13C) shows central allene C at 196.25 ppm.

En like manner, 9-(4',5',51,6'-tetradehydro- 51',6' ,dideoxy-2' ,3'-0-ethoxymethylidene-f3-D-ribohexofuranosyl)-9-H-purin-6-amine can be prepared from 9- (4',5',5',6'-tetradehydro-5',6'dideoxy-2',3'-0ethoxymethylidene-3-D--ribo-hexofuranosyl )-9-H-o~ur in-6amine which is prepared by reacting the appropriate starting material with triethylorthoformate in trichloroacetic acid and following the procedures set forth in Scheme A.

Step b: 9-(4',5',5',6'-tetradehydro-5',6'-dideoxy-o3-Dribo-hexofuranosyl) -9-H-pu,,rin-6-amine Add ,6'-tetradehydro-5' ,6'-dideoxy-2' isopropylidene-p-D-ribo-hexofuranosyl )-9-H-purin-6-amine mg) and PdCl 2

(CH

3

CN)

2 (5 mg) to aqueous acetonitrile ml) and stir until the reaction is complete. Evaporate the reaction mixture and recrystallize the product from methanol to yield the title compound as a white solid.

Where the 21,31-0-ethoxymethylidene derivative is employed, stir a solution of ,6'-tetradehydro- ,6'-dideoxy-2' ,3'-0-ethoxymethylidene-3-Dribohexofuranosyl)-9-H-purin-6-amine (50 mg) and pyridinium R-toluenesulfonate (50 mg) in ethanol (12 ml) and H120 (6 ml) at 40 0 C until the ethoxymethylidene protecting groups are cleaved. Evaporate the reaction mixture and recrystallize the product from methanol.

M01295 -24- The following specific compounds can be made by procedures analogous to those described above in Example 3: lR-(l.alpha., 2.alpha, 3.beta.)-3-(9H-purin-6-amine-9-yl)- 5-propadienyl-l, 2-cyclopentanediol 3-(4 ,51,5' ,61-tetradehydro-5' ,6'-dideoxy-p-Dribohexofuranosyl)-3H-imidazol4,5-blpyridin-7-amine ,6'-tetradehydro-5' ,6'-dideoxy-4'-thio-3-Dribohexofuranosyl)-3H-imidazo(4, 5-blpyridin-7-amine ,5 ,61-tetradehydro-5' ,6'-dideoxy--4'-thio-f3-Dribohexofuranosyl )-9H-purin-6-amine An alternative procedure for preparing acetylenic, cyano and allenic adenosine derivatives of the formula (1) or (1a) is set forth in Scheme D. This method involves preparing the adenosyl base and ribosyl moieties separately and then effecting a condensation of the moieties.

M01295 SCHEME D HC-- C OCH3 H AlB H

H

OB A 2 8 HC- C step a H OH H Al

H

OH A2 (16) HC C

H

step b OAc A2B (17) (17) Y

N

S (1Y3

H

(18) HC C ste H step c

Y

1

N

H A 1 8

H

OAc A2B

NY

2

Y

3 Acetylenic ribosyl derivatives (15) are prepared according to standard techniques and procedures which are well known and appreciated by those of ordinary skill in the art. For example, these compounds can be prepared from the appropriate ribosyl derivatives by methods analogous to those described in Scheme A. Appropriatstarting materials are readily available.

M01295 -26- These derivatives (15) are hydrolyzed in step a using an acid such as acetic acid. The hydrolyzed derivatives (16) are subsequently converted to the corresponding acetic acid esters (17) in step b by reaction with acetic anhydride in pyridine.

Procedures for making the adenine derivative (18) also involve standard techniques and procedures which are well known and appreciated by those of ordinary skill in the art.

The acetic acid ester (17) can be condensed with the a, appropriate adenine derivative (18) through a fusion reaction or through a condensation reaction in the presence of bis-trimethylsilylacetamide and a Lewis acid Ssuch as trimethylsilyltrifluoromethanesulfonate.

The condensed product can then be de-blocked by hydrolysis as described in Scheme A (steps e and f) or further reacted to provide compounds of formula (la) as ,o described in Scheme C.

Compounds of formula wherein R1 is cyano can also be prepared according to methods analogous to those described in Scheme D.

o Starting materials for use in the general synthetic Sprocedure outlined in Scheme C are readily available to oone of ordinary skill in the art. For example, the starting materials for various compounds of formula or (e (la) are listed in Table 2.

M01295 -27- I-1 TABLE 2 Examples of Starting Materials for Scheme D Compound of formula or (la) wherein Source of V Ai A2 Y 1

Y

2

Y

3 Z Q Starting Material S0O H OH CH N N CI NH 2 2-Chloroadenine and Tet. Lett.

1977,3433 O H OH CH N N H NH 2 Adenine CH2 H OH CH N CH H NH 2 3-deazaadenine Additional starting materials can be prepared by the use of methods analogous to those described in Table 2 as well as other conventional methods as are well known and appreciated in the art.

In another embodiment, the present invention provides a method of inhibiting AdoMet-dependent transmethylation activity in a patient in need thereof which comprises administration of a compound of the formula or (la) in an effective inhibitory amount. The term "effective inhibitory amount" refers to an amount sufficient to inhibit the AdoMet-dependent transmethylation activity after single or multiple dose administration.

As used herein, the term "patient" refers to a warmblooded animal such as a mammal which is afflicted with a particular disease state. It is understood that dogs, cats, rats, mice, horses, bovine cattle, sheep, and humans are examples of animals within the scope of the meaning of ,7 the term.

M01295 -28- _r The compounds of formula or (la) are believed to exert their inhibitory effect on AdoMet-dependent transmethylation by inhibition of AdoHcy Hydrolase thereby providing an increase in tissue levels of AdoHcy which in turn provides feedback inhibition of AdoMet-dependent transmethylation. However, it is understood that the present invention is not limited by any particular theory or proposed mechanism to explain its effectiveness in an end-use application.

As is well known and appreciated by those skilled in the art, various disease states, such as certain neoplastic disease states and viral infections, are characterized by excessive Adomet-dependent transmethylation activity. As used herein, the term "excessive" means a level of activity which allows the disease state to progress.

More specifically, the present invention provides a method for the treatment of a patient afflicted with a neoplastic disease state which is characterized by excessive AdoMet dependent transmethylation activity comprising the administration of an effective antineoplastic amount of a compound of formula or (la).

The term "neoplastic disease state" as used herein refers to an abnormal state or condition characterized by rapidly proliferating cell growth or neoplasm. Neoplastic disease states which are characterized by an excessive AdoMetdependent tr.nsmethylation activity and for which treatment with a compound of formula or (la) will be particularly useful include: Leukemias such as, but not limited to, acute lymphoblastic, chronic lymphocytic, acute myloblastic and chronic mylocytic; Carcinomas, such as, but not limited to, those of the cervix, oesophagus, stomach, small intestines, colon and lungs; Sarcomas, such M01295 -29- -1 LI-i rr~ as, but not limited to, oesteoma, osteosarcoma, lipoma, liposarcoma, hemangioma and hemangiosarcoma; Melanomas, including amelanotic and melanotic; and mixed types of neoplasias such as, but not limited to carcinosarcoma, lymphoid tissue type, folicullar reticulum, cell sarcoma and Hodgkins Disease.

An effective antineoplastic amount of a compound of formula or (la) refers to an amount which is effective, upon single or multiple dose administration to the patient, in controlling the growth of the neoplasm or in prolonging the survivability of the patient beyond that expected in the absence of such treatment. As used herein, "controlling the growth" of the neoplasm refers to slowing, interrupting, arresting or stopping its growth and metastases and does not necessarily indicate a total elimination of the neoplasm.

In addition, the present invention provides a method for the treatment of a patient afflicted with a viral infection which is characterized by excessive AdoMetdependent transmethylation activity comprising the administration of an effective antiviral amount of a compound of formula or The term "viral infection" as used herein refers to an abnormal state or condition characterized by viral transformation of cells, viral replication and proliferation. Viral infections which are characterized by an excessive AdoMet dependent transmethylation activity and for which treatment with a compound of formula or (la) will be particularly useful include: Retroviruses such as, but not limited to, HTLV-I, HTLV-IT, human immunodeficiency viruses, HTLV-III (AIDS virus), and the like; RNA viruses such as, but not limited to, influenza type A, B, and C, mumps, measles, rhinovirus, dengue, rubella, rabies, hepatitis virus A, M01295 i ~cl 1 I encephalitis virus, and the like; DNA viruses such as, but not limited to, herpes, vaccinia, pappiloma virus (wart), hepatitis virus B, and the like.

An effective antiviral amount of a compound of formula or (la) refers to an amount which is effective in controlling the virus. This viral control refers to slowing, interrupting, arresting or stopping the viral transformation of cells or the replication and proliferation of the virus and does not necessarily B 10 indicate a total elimination of the virus.

An effective dose can be readily deterrained by the attending diagnostician, as one skilled in the art, by the use of conventional techniques and by observing results obtained under analogous circumstances. In determining the effective dose, a number of factors are considered by the attending diagnostician, including, but not limited to: the species of mammal; its size, age, and general health; the specific disease involved; the degree of or involvement or the severity of the disease; the response of the individual patient; the particular compound administered; the mode of administration; the bioavailability characteristics of the preparation administered; the dose regimen selected; the use of concomitant medication; and other relevant circumstances.

M01295 -31i Effective antineoplastic and antiviral amounts of a compound of formula or (la) are expected to vary from about 0.1 milligram per kilogram of body weight per day (mg/kg/day) to about 100 mg/kg/day. Preferred amounts are expected to vary from about 0.5 to about 10 mg/kg/day.

In an additional embodiment, the present invention relates to a method of treating a patient afflicted with a neoplastic disease state or a viral infection comprising administration of an effective antineoplastic or antiviral amount of a compound of formula or (la) wherein Q is "Io NH 2 in conjunctive therapy with an effective inhibitory o amount of an Adenosine Deaminase (ADA) inhibitor. The term "conjunctive therapy" contemplates coadministration of or (la) along with an ADA inhibitor at essentially S: 15 the same time, or treatment of the patient with an ADA inhibitor prior to or after treatment with a compound of formula or An effective inhibitory amount of an ADA inhibitor is an amount effective in significantly o inhibiting ADA in the patient.

ADA deaminates compounds of formula or (la) wherein Q is NH 2 and thereby degrades the active compounds to relatively inactive metabolites. When a compound of formula or (la) wherein Q is NH 2 and an ADA inhibitor are administered in conjunctive therapy, the dose will be S 25 less in amount or frequency of administration than that required when the compound of formula or (la) is administered alone.

SVarious pharmaceutically acceptable non-toxic ADA inhibitors can be used including, but not limited to, deoxycoformycin. An effective inhibitory amount of the ADA inhibitor will vary from about 0.05 mg/kg/day to about mg/kg/day and preferably will be from about 0.1 M01295 mg/kg/day to about 0.3 mg/kg/day. Deoxycoformycin is the preferred ADA inhibitor for use in conjunctive therapy with compounds of formula or (la) wherein Q is NH 2 In effecting treatment of a patient afflicted with a disease state described above, a compound of formula (1) or (la) can be administered in any form or mode which makes the compound bioavailable in effective amounts, including oral and parenteral routes. For example, compounds of formula or (la) can be administered orally, subcutaneously, intramuscularly, intravenously, Stransdermally, intranasally, rectally, and the like. Oral administration is generally preferred. One skilled in the art of preparing formulations can readily select the proper form and mode of administration depending upon the particular characteristics of the compound selected the disease state to be treated, the stage of the disease, and other relevant circumstances.

o The compounds can be administered alone or in the form of a pharmaceutical composition in combination with pharmaceutically acceptable carriers or excipients, the proportion and nature of which are determined by the solubility and chemical properties of the compound selected, the chosen route of administration, and standard pharmaceutical practice. In addition, compounds of formula or (la) wherein Q is NH 2 can be administered as above in further combination with an ADA inhibitor.

The compounds of the invention, while effective themselves, may be formulated and administered in the form of their pharmaceutically acceptable acid addition salts for purposes of stability, convenience of crystallization, increased solubility and the like, M0129 -33 M01295 -33- In another embodiment, the present invention provides a pharmaceutical composition comprising an effective amount of a compound of formula or (la) in admixture or otherwise in association with one or more pharmaceutically acceptable carriers or excipients. In addition, the present invention provides a pharmaceutical composition comprising an effective amount of a compound of formula or (la) wherein Q is NH 2 and an effective ADA inhibitory amount of an ADA inhibitor in admixture or otherwise in association with one or more pharmaceutically acceptable carriers or excipients. The term "effective o amounts" as applied to compounds of formula or (la) refers to effective inhibitory, antineoplastic, or o antiviral amounts as appropriate.

0 S 15 The pharmaceutical compositions are prepared in a Smanner well known in the pharmaceutical art. The carrier or excipient may be a solid, semi-solid, or liquid material which can serve as a vehicle or medium for the active ingredient. Suitable carriers or excipients are well known in the art. The pharmaceutical composition may be adapted for oral or parenteral use and may be administered to the patient in the form of tablets, capsules, suppositories, solution, suspensions, or the like, The compounds of the present invention may be administered orally, for example, with an inert diluent or with an edible carrier. They may be enclosed in gelatin capsules or compressed into tablets. For the purpose of oral therapeutic administration, the compounds may be incorporated with excipients and used in the form of tablets, troches, capsules, elixirs, suspensions, syrups, wafers, chewing gums and the like. These preparations should contain at least 4% of the compound of the M0129 -34 M01295 -34vt *-utosMrf invention, the active ingredient, but may be varied depending upon the particular form and may conveniently be between 4% to about 70% of the weight of the unit. The amount of the compound present in compositions is such that a suitable dosage will be obtained. Preferred compositions and preparations according to the present invention are prepared so that an oral dosage unit form contains between 5.0-300 milligrams of a compound of the invention.

The tablets, pills, capsules, troches and the like may 0. also contain one or more of the following adjuvants: binders such as microcrystalline cellulose, gum tragacanth or gelatin; excipients such as starch or lactose, disintegrating agents such as alginic acid, Primogel, corn starch and the like; lubricants such as magnesium stearate or Sterotex; glidants such as colloidal silicon dioxide; and sweetening agents such as sucrose or saccharin may be added or a flavoring agent such as peppermint, methyl salicylate or orange flavoring. When the dosage unit form is a capsule, it may contain, in addition to materials of the above type, a liquid carrier such as polyethylene glycol or a fatty oil. Other dosage unit forms may contain other various materials which modify the physical form of the dosage unit, for example, as coatings. Thus, 25 tablets or pills may be coated with sugar, shellac, or o other enteric coating agents. A syrup .y contain, in addition to the present compounds, sucrose as a sweetening agent and certain preservatives, dyes and colorings and flavors. Materials used ir preparing these various compositions should be pharmaceutically pure and non-toxic in the amounts used.

For the purpose of parenteral therapeutic administration, such as intramuscular, intravenous, and M01295 subcutaneous, the compounds of the present invention may be incorporated into a solution or suspension. These preparations should contain at least 0.1% of a compound of the invention, but may be varied to be between 0.1 and about 50% of the weight thereof. The amount of the inventive compound present in such compositions is such that a suitable dosage will be obtained. Preferred compositions and preparations according to the present invention are prepared so that a parenteral dosage unit contains between 5.0 to 100 milligrams of the compound of the invention.

The solutions or suspensions may also include one or more of the following adjuvants: sterile diluents such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl paraben; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as Sethylene diaminetetraacetic acid; buffers such ,_s i 20 acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. The parenteral preparation can be enclosed in ampules, disposable syringes or multiple dose vials made of glass or plastic.

Any of the above described pharmaceutical compositions containing compounds of formula or (la) wherein Q is

NH

2 may also contain an effective inhibitory amount of an ADA inhibitor in admixture or otherwise in association with the above described ingredients.

As with any group of structurally related compounds which posses a particular generic utility, certain groups M01295 -36and configurations are preferred for compounds of formula or (la) in their end-use application.

With respect to the substituent R 1 compounds wherein

R

1 is ethynyl or cyano are generally preferred. With respect to the substituent R2, compounds wherein R 2 is hydrogen are generally preferred.

With respect to the substituents Ai and A 2 compounds wherein one of A 1 and A2 is hydroxy and the other is hydrogen are generally preferred. Compounds wherein Aj is hydrogen and A 2 is hydroxy are especially preferred.

The following are additional preferred embodiments: Scompounds of formula wherein V is oxy, compounds of formula (la) wherein V is methylene or thio, compounds of 1 formula or (la) wherein Y 1 is a CH group, compounds of S 15 formula or (la) wherein Y 2 is nitrogen, compounds of formula or (la) wherein Y 3 is nitrogen and compounds t of formula or (la) wherein Z is hydrogen. Finally, with respect to Q, those compounds of formula or (la) wherein Q is NH2 or NHCH 3 are generally preferred with those wherein Q is NH2 being especially preferred.

The following list identifies compounds of the formula and (la) which are particularly preferred embodiments of the present invention: 9-(5'5,6',6'-Tetradehydro-5',6'-dideoxy-B-D-ribohexofuranosyl)-9H-purin-6-amine ,6',6'-Tetradehydro-5',6'-dideoxy-8-D-ribohexofuranosyl)-9H-purin-6-amine 9-(8-D-eibofuranonitrile)-9H-purin-6-amine M01295 -37i a-D-arabinofuranonitrile)-9H-purin-6-amine ,51,5' ,6'-Tetradehydro-5' ,6'-dideoxy-4'-thio-a-Dribohexofuranosyl.) -9H-purin-6-anine The above list is intended to be merely illustrative of particularly preferred embodiments of the present invention and it is understood that the list does not limit the scope of the invention in any way.

M01.295 -8 -38-

Claims

3. A compound as claimed in Claim 1 wherein R1 is ethynyl.
4. A compound as claimed in Claim 1 wherein R 1 is cyano. A compound as claimed in any one of Claims 1 to 4 wherein V is oxy.
6. A compound as claimed in Claim 2 wherein V is methylene. c.o o *.0 0r 0 o *0 0 0 0 00 00 000 00' i_- KC -41- A compound as claimed in Claim 2 or Claim 3 wherein R2 is hydrogen.
8. A compound as claimed in any one of Claims 1 to 7 wherein A 2 is hydroxy.
9. A compound as claimed in any one of Claims 1 to 8 wherein A 1 is hydroxy. A compound as claimed in any one of Claims 1 to 9 wherein Y1 is a CH group.
11. A compound as claimed in any one of Claims 1 to wherein Y2 is nitrogen.
12. A compound as claimed in any one of Claims 1 to 11 wherein Y3 is nitrogen.
13. A compound as claimed in any one of Claims 1 to 12 wherein Z is hydrogen.
14. A method of inhibiting AdoMet-dependent transmethylation activity in a patient in need thereof comprising administration of an effective inhibitory amount of a compound as claimed in any one of Claims 1 to 13. A method of treating a patient afflicted with a neoplastic disease state comprising the administration of an effective antineoplastic amount of a compound as claimed in any one of Claims I to 13.
16. A method of treating a patient afflicted with a viral infection comprising the administration of an effective antiviral amount of a compound as claimed in any one of Claims 1 to 13.
17. A method of controlling the growth of a neoplasm in a patient afflicted with a neoplastic disease state comprising administration of an effective antineoplastic amount of a compound as claimed in any one of Claims I to 13. ]KC -42-
18. A method of controlling the growth of mammalian cells characterized by the presence of a transmethylation dependent disease stat-. comprising exposing such cells to an effective inhibitory amount of a compound as claimed in any one of Claims 1 to 13.
19. A compound as claimed in Claim 1 wherein the compound is 9- (5 ,5 ,6',6'-tetradehydro-5 ,6'-dideoxy-3-D-ribo- hexofuranosyl)-9-H-purin-6-amine. A compound as claimed in Claim 1 wherein the compound is 9-(I-D-ribofuranonitrile)-9H-purin-6-amine. 000 00 21. A compound as claimed in Claim 1 wherein the compound is 9-(8-D-arabinofuranonitrile)--911 pturin-6-amine.
22. A compound as claimed in Claim 1 wherein the compound is adchydro-5',6'--dideoxy-B-D- ribofuranosyl)-3H-l,2,3-tridzolot4,5dpyrimidin-7-amine.
23. A compound as claimed in Claim 1 wherein the compound is l 5 etradehydro-5,6'-dideoxy-B-D- 00 4 rib fu ranosyl) H- imidazot4, c I pyridin-4 amine.
24. A compound as claimed in Claim I wherein the compound is 9-(5 ,5 ,6 ,-Letriideydro-i dideoxy-4 -thio -VOD- ribo-hexofuranosyl)-..9Wpurin- 6aznine. A compound as claimed in Claim I wherein the compound is 9-(51,5-,6 ,'t-ratryr.h5,6-id-xy"D-rbio hexofuranosyl)-9H-purin.-6amine.
26. A compound as claimed in Claim 1 wherein the compound amine-9-yl)-5-ethynyl-12-cyclopentanediol.
27. A compound as claimed in Claim I wherein the compound is 9-(4-thio-4-D-ribofur inonitrile) -91-purin-6-amine. INC ur -A 1 Li*FC-~~ -43
28. A compound as claimed in Claim 1 wherein the compound is 1R-(1.alpha., 2.alpha., 3. beta., 5. beta.) -3-(9H-purin-6- amine-9-yl)-5-cyano-1,2-cyclopentanediol.
29. A compound as claimed in Claim 1 wherein the compound is 1-(B-D-ribofuranonitrile)-lH-imidazo[4,5-c]pyridin-4- amine. A compound as claimed in Claim 2 wherein the compound is 1R-(1. alpha., 2. alpha, 3. beta.) -3 -(9H-purin-6-amine--9-yl)- 5-propadienyl-1,2-cyclopentanediol.
31. A compound as claimed in Claim 2 wherein the compound is 3-(4',5',5',6'-etradehydro-5,6'-dideoxy-3-D- ribohexofuranosyl)-3H..imidazo[4,5-b)pyridin-7-amine.
32. A compound as claimed in Claim 2 wherein the compound is 3-(4,5 t tra a hyd ro- ,6 i oxy-4 -thio-B- n ribohexofuranosyl)-31l-imidazo[4,5 b-pyridin-7-amine.
33. A compound as claimed in Claim 2 wherein the compound is 9-(4',5,5,6t'raetlidr-5,6oieoy-'-hi-e-D ribohexofuranosyl)-9-purin-6arnjne.
34. A compound as claimed in Claim 2 wherein the compound is 9-(4 -totr adhydro-5, 6-didexy-13 D-r bo- hexofuranosyl)-9ip-purin-6-amine. A process for making an aristoeromycin/adenosine derivative of any one of claims 1, 3 to 5, 8 to 13 or 19 to 29 or a pharmaceutically acceptable salt thereof, comprising treating with an, acid an aristeromycin/adenosine derivative of the formula K(C i -44- 'N 72 V oS wherein V, R 1 Al, A 2 Y 1 Y 2 Y 3 Q and Z are as defined, 0 represents a protected hydroxy group, and A 1 and A 2 represent protected hydroxy groups when Al or A 2 is hydroxy and represent A 1 or A 2 when Al or A 2 are other than hydroxy.
36. A process for making an aristeromycin/adenosine derivative of any one of claims 2, 6 to 13 or 30 to 34 or a pharmaceutically acceptable salt thereof, comprising treating with an acid an aristeromycin/adenosine derivative Fr of the formula 1i A 1 C I KC: I wherein V, R 2 I- A 2 Y 1 Y2' Y3, Q and Z B are as defined, 0 represents a protected hydroxy group, and B B Al and A 2 represent protected hydroxy groups when Al or A 2 is hydroxy and represent Al or A 2 when A 1 or A are other than hydroxy. 3P.*7 A compound as claimed in claim 1 or 2 substantially as hereinbefore described with reference to any one of the examples. 3 or 3 34. A process as claimed in clainis4:36 U.e, substantially as hereinbefore described with reference to any one of the examples. DATED: 28 March, 1989 PHILLIPS ORMONDE FITZPATRICK Attorneys for: MERRELL DOW PHARMACEUTICALS INC. .iC f v- i";sbi~r 7 45