AU4314099A - Novel method of large scale plasmid purification - Google Patents

Novel method of large scale plasmid purification

Info

Publication number
AU4314099A
AU4314099A AU43140/99A AU4314099A AU4314099A AU 4314099 A AU4314099 A AU 4314099A AU 43140/99 A AU43140/99 A AU 43140/99A AU 4314099 A AU4314099 A AU 4314099A AU 4314099 A AU4314099 A AU 4314099A
Authority
AU
Australia
Prior art keywords
large scale
novel method
plasmid purification
scale plasmid
purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU43140/99A
Inventor
William N. Brune
Roy Musil
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Immune Response Corp
Original Assignee
Immune Response Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immune Response Corp filed Critical Immune Response Corp
Publication of AU4314099A publication Critical patent/AU4314099A/en
Abandoned legal-status Critical Current

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20Anion exchangers for chromatographic processes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30Processes for preparing, regenerating, or reactivating
    • B01J20/32Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3285Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361Ion-exchange
    • B01D15/363Anion-exchange
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3847Multimodal interactions
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Peptides Or Proteins (AREA)
AU43140/99A 1998-06-01 1999-05-26 Novel method of large scale plasmid purification Abandoned AU4314099A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US8756898P 1998-06-01 1998-06-01
US60087568 1998-06-01
US23846699A 1999-01-28 1999-01-28
US09238466 1999-01-28
PCT/US1999/011588 WO1999063076A1 (en) 1998-06-01 1999-05-26 Novel method of large scale plasmid purification

Publications (1)

Publication Number Publication Date
AU4314099A true AU4314099A (en) 1999-12-20

Family

ID=26777125

Family Applications (1)

Application Number Title Priority Date Filing Date
AU43140/99A Abandoned AU4314099A (en) 1998-06-01 1999-05-26 Novel method of large scale plasmid purification

Country Status (2)

Country Link
AU (1) AU4314099A (en)
WO (1) WO1999063076A1 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
IL147644A0 (en) 1999-07-23 2002-08-14 Genentech Inc Method for rnase-and organic solvent-free plasmid dna purification using tangential flow filtration
ATE278699T1 (en) * 2000-02-16 2004-10-15 Macherey Nagel Gmbh & Co Hg METHOD FOR PURIFYING NUCLEIC ACIDS AND ANION EXCHANGER FOR CARRYING OUT THIS METHOD
DE10010342A1 (en) * 2000-03-06 2001-09-20 Merck Patent Gmbh Method for reducing the endotoxin content of nucleic acid (I) is derived from natural, genetic engineering or biotechnological sources is used to produce high-purity plasmid DNA from natural sources
EP1290158B1 (en) * 2000-06-02 2006-12-06 Pall Corporation Processing of plasmid-containing fluids
EP1243649A1 (en) * 2001-03-23 2002-09-25 GeneScan Europe AG Method for the isolation of DNA
US7314746B2 (en) 2002-09-13 2008-01-01 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
CA2530454A1 (en) * 2003-06-24 2005-01-13 Akzo Nobel N.V. Removal of lipopolysaccharides from protein- lipopolysaccharide complex es by nonflammable solvents
GB0323814D0 (en) * 2003-10-10 2003-11-12 Cobra Biolog Ltd Purification of biological macromolecules
SE0400490D0 (en) * 2004-02-26 2004-02-26 Amersham Biosciences Ab Plasmid purification
US7531308B2 (en) * 2004-04-23 2009-05-12 Sigma-Aldrich Co. Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography
WO2009063069A2 (en) * 2007-11-15 2009-05-22 Novo Nordisk A/S Endotoxin removal
BR112017005866A2 (en) 2014-09-24 2018-02-06 Basf Se A process for preparing a terephthalic diester by reacting terephthalic acid with at least one alcohol.
WO2016046120A1 (en) 2014-09-24 2016-03-31 Basf Se Method for producing diesters of terephthalic acid with enrichment of recirculated alcohol
TR201905052T4 (en) 2014-09-24 2019-05-21 Basf Se Method for producing terephthalic diesters by circulation of the reaction mixture.
TR201904928T4 (en) 2014-09-24 2019-05-21 Basf Se Method for the production of terephthalic acid diesters by recirculating alcohol dehydration.
DE102016106271B4 (en) 2016-04-06 2018-03-29 Axagarius Gmbh & Co. Kg Purification of nucleic acid from a nucleic acid and endotoxin containing sample
CN111304193A (en) * 2020-02-28 2020-06-19 恒瑞源正(上海)生物科技有限公司 Method for large-scale rapid purification of plasmid DNA
EP3906982B1 (en) 2020-05-08 2022-03-16 AXAGARIUS GmbH & Co. KG Plasma cleaning method with simultaneous depletion of endotoxins
CN112322612A (en) * 2020-10-29 2021-02-05 江苏凯基生物技术股份有限公司 Plasmid extraction kit and extraction method

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3644346A1 (en) * 1986-12-19 1987-05-21 Saeulentechnik Dr Ing Herbert Matrix-bound crown ether ligands as separating material in the affinity chromatography of nucleic acids
AR003122A1 (en) * 1995-05-19 1998-07-08 Merck & Co Inc A PROCESS FOR ISOLATION AND PURIFICATION OF PLASMIDS IN LARGE SCALE FERMENTATORS AND ISOLATED AND PURIFIED DNA OBTAINED THROUGH SUCH A PROCESS.

Also Published As

Publication number Publication date
WO1999063076A1 (en) 1999-12-09

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase