AU3699584A - Purification of recombinant interleukin-2 - Google Patents

Purification of recombinant interleukin-2

Info

Publication number
AU3699584A
AU3699584A AU36995/84A AU3699584A AU3699584A AU 3699584 A AU3699584 A AU 3699584A AU 36995/84 A AU36995/84 A AU 36995/84A AU 3699584 A AU3699584 A AU 3699584A AU 3699584 A AU3699584 A AU 3699584A
Authority
AU
Australia
Prior art keywords
purification
recombinant interleukin
lysis
chromatography
extract
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU36995/84A
Other versions
AU580276B2 (en
Inventor
Hsiang-Fu Kung
Shigeko Yamazaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of AU3699584A publication Critical patent/AU3699584A/en
Application granted granted Critical
Publication of AU580276B2 publication Critical patent/AU580276B2/en
Assigned to F. HOFFMANN-LA ROCHE AG reassignment F. HOFFMANN-LA ROCHE AG Alteration of Name(s) in Register under S187 Assignors: F. HOFFMANN-LA ROCHE AG
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Homogeneous recombinant human interleukin-2 is prepared by extraction of IL-2 from the cell membrane components of transformed microorganisms after lysis and purification of the extract by chromatography. <IMAGE>
AU36995/84A 1983-12-23 1984-12-20 Purification of recombinant interleukin-2 Expired AU580276B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56498683A 1983-12-23 1983-12-23
US564986 1983-12-23

Publications (2)

Publication Number Publication Date
AU3699584A true AU3699584A (en) 1985-07-04
AU580276B2 AU580276B2 (en) 1989-01-12

Family

ID=24256731

Family Applications (1)

Application Number Title Priority Date Filing Date
AU36995/84A Expired AU580276B2 (en) 1983-12-23 1984-12-20 Purification of recombinant interleukin-2

Country Status (12)

Country Link
EP (1) EP0147819B1 (en)
JP (2) JPH0728753B2 (en)
AT (1) ATE76905T1 (en)
AU (1) AU580276B2 (en)
CA (1) CA1340853C (en)
DE (1) DE3485759D1 (en)
DK (1) DK174501B1 (en)
IE (1) IE57914B1 (en)
IL (1) IL73882A (en)
NZ (1) NZ210634A (en)
PH (1) PH19584A (en)
ZA (1) ZA849910B (en)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4925919A (en) * 1984-04-25 1990-05-15 Roland Mertelsmann Purified interleukin 2
US4992271A (en) * 1982-09-23 1991-02-12 Cetus Corporation Formulation for lipophilic IL-2 proteins
US4853332A (en) * 1982-10-19 1989-08-01 Cetus Corporation Structural genes, plasmids and transformed cells for producing cysteine depleted muteins of biologically active proteins
JPS60115528A (en) * 1983-11-28 1985-06-22 Takeda Chem Ind Ltd Human interleukin-2 protein, its production and pharmacological composition containing the same
US4569790A (en) * 1984-03-28 1986-02-11 Cetus Corporation Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
US4908434A (en) * 1984-04-25 1990-03-13 Sloan-Kettering Institute For Cancer Research Process for preparing purified interleukin-2
US4908433A (en) * 1984-04-25 1990-03-13 Sloan-Kettering Institute For Cancer Research Uses of interleukin-2
WO1986002068A1 (en) * 1984-09-26 1986-04-10 Takeda Chemical Industries, Ltd. Mutual separation of proteins
IL76360A0 (en) * 1984-09-26 1986-01-31 Takeda Chemical Industries Ltd Mutual separation of proteins
WO1986007093A1 (en) * 1985-05-29 1986-12-04 The Green Cross Corporation Process for preparing heterogenic protein
US4748234A (en) * 1985-06-26 1988-05-31 Cetus Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
WO1987000553A1 (en) * 1985-07-16 1987-01-29 The Green Cross Corporation Process for preparing hetero-protein
US5831022A (en) * 1986-02-18 1998-11-03 Hoffmann-La Roche Inc. Purification of recombinant human IL-1α
CA1339757C (en) 1987-04-16 1998-03-17 Robert F. Halenbeck Production of purified biologically active, bacterially produced recombinant human csf-1
US4929700A (en) * 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
US4931543A (en) * 1987-05-11 1990-06-05 Cetus Corporation Process for recovering microbially produced interleukin-2
US5162507A (en) * 1987-05-11 1992-11-10 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US5162503A (en) * 1987-05-19 1992-11-10 Hoffmann-La Roche, Inc. Purification of interleukin-2 by receptor-affinity chromatography
EP0337243A1 (en) * 1988-04-14 1989-10-18 F. Hoffmann-La Roche Ag Process for purifying recombinant human interleukin-2
ZA898139B (en) * 1988-11-17 1990-08-29 Hoffmann La Roche Recombinant interleukin-2 hybrid proteins
IT1262981B (en) * 1992-09-09 1996-07-23 Tecnogen Scpa PROCEDURE FOR PURIFYING THE BIG ENDOTELINE PROTEIN
US7144999B2 (en) * 2002-11-23 2006-12-05 Isis Pharmaceuticals, Inc. Modulation of hypoxia-inducible factor 1 alpha expression
JP2006141263A (en) * 2004-11-18 2006-06-08 Kyoto Univ Method for producing (r)-hydroxynitril lyase
RU2565553C2 (en) * 2013-04-09 2015-10-20 Общество с ограниченной ответственностью "Стратегия" Method of obtaining interleukin-2 preparation, interleukin-2 preparation and pharmaceutical immunomodulating preparation
CN106604932A (en) 2014-07-10 2017-04-26 诺华公司 Immune-stimulating monoclonal antibodies against human interleukin-2
KR20180100224A (en) 2016-01-11 2018-09-07 노바르티스 아게 Immune-stimulated humanized monoclonal antibodies against human interleukin-2, and fusion proteins thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58159420A (en) * 1982-03-18 1983-09-21 Ajinomoto Co Inc Purification method of interleukin 2
DE3377363D1 (en) * 1982-03-31 1988-08-18 Ajinomoto Kk Gene coding for interleukin-2 polypeptide, recombinant dna carrying said gene, cell lines possessing the recombinant dna,and method for producing interleukin-2 using said cells
DE3378128D1 (en) * 1982-04-20 1988-11-03 Sloan Kettering Inst Cancer Purification of interleukin 2
JPS58198293A (en) * 1982-05-12 1983-11-18 Shionogi & Co Ltd Production of immunoregulator such as interleukin
JPS59144719A (en) * 1983-02-03 1984-08-18 Japan Found Cancer Gene coding interleukin-2 polypeptide, recombined dna substance containing gene, and preparation of interleukin-2 using live cell strain containing dna substance and cell
IL71275A0 (en) * 1983-03-21 1984-06-29 Sparamedica Ag Human interleukin-2-and its preparation
JPS60115528A (en) * 1983-11-28 1985-06-22 Takeda Chem Ind Ltd Human interleukin-2 protein, its production and pharmacological composition containing the same
US4569790A (en) * 1984-03-28 1986-02-11 Cetus Corporation Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
DE3419995A1 (en) * 1984-05-29 1985-12-05 Hoechst Ag, 6230 Frankfurt GENE TECHNOLOGICAL METHOD FOR PRODUCING HUMAN INTERLEUKIN-2 AND MEANS FOR CARRYING OUT THIS METHOD

Also Published As

Publication number Publication date
JPH0728753B2 (en) 1995-04-05
ATE76905T1 (en) 1992-06-15
EP0147819A3 (en) 1987-09-23
IL73882A0 (en) 1985-03-31
EP0147819B1 (en) 1992-06-03
CA1340853C (en) 1999-12-21
JP2864996B2 (en) 1999-03-08
IE843320L (en) 1985-06-23
DK612484D0 (en) 1984-12-19
IE57914B1 (en) 1993-05-19
AU580276B2 (en) 1989-01-12
NZ210634A (en) 1989-05-29
EP0147819A2 (en) 1985-07-10
JPH07233082A (en) 1995-09-05
JPS60172295A (en) 1985-09-05
DE3485759D1 (en) 1992-07-09
IL73882A (en) 1989-05-15
ZA849910B (en) 1985-09-25
PH19584A (en) 1986-05-26
DK174501B1 (en) 2003-04-28
DK612484A (en) 1985-06-24

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