AU2180500A - Use of a mek inhibitor for preventing transplant rejection - Google Patents

Use of a mek inhibitor for preventing transplant rejection Download PDF

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AU2180500A
AU2180500A AU21805/00A AU2180500A AU2180500A AU 2180500 A AU2180500 A AU 2180500A AU 21805/00 A AU21805/00 A AU 21805/00A AU 2180500 A AU2180500 A AU 2180500A AU 2180500 A AU2180500 A AU 2180500A
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methyl
iodo
phenylamino
benzamide
difluoro
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AU21805/00A
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Richard Buell Gilbertsen
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Warner Lambert Co LLC
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Warner Lambert Co LLC
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/535—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one oxygen as the ring hetero atoms, e.g. 1,2-oxazines
    • A61K31/5375—1,4-Oxazines, e.g. morpholine
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
    • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/13—Amines
    • A61K31/135—Amines having aromatic rings, e.g. ketamine, nortriptyline
    • A61K31/136—Amines having aromatic rings, e.g. ketamine, nortriptyline having the amino group directly attached to the aromatic ring, e.g. benzeneamine
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/16—Amides, e.g. hydroxamic acids
    • A61K31/165—Amides, e.g. hydroxamic acids having aromatic rings, e.g. colchicine, atenolol, progabide
    • A61K31/166—Amides, e.g. hydroxamic acids having aromatic rings, e.g. colchicine, atenolol, progabide having the carbon of a carboxamide group directly attached to the aromatic ring, e.g. procainamide, procarbazine, metoclopramide, labetalol
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    • A61K31/16—Amides, e.g. hydroxamic acids
    • A61K31/18—Sulfonamides
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
    • A61K31/196—Carboxylic acids, e.g. valproic acid having an amino group the amino group being directly attached to a ring, e.g. anthranilic acid, mefenamic acid, diclofenac, chlorambucil
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/34—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having five-membered rings with one oxygen as the only ring hetero atom, e.g. isosorbide
    • A61K31/341—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having five-membered rings with one oxygen as the only ring hetero atom, e.g. isosorbide not condensed with another ring, e.g. ranitidine, furosemide, bufetolol, muscarine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/38—Heterocyclic compounds having sulfur as a ring hetero atom
    • A61K31/381—Heterocyclic compounds having sulfur as a ring hetero atom having five-membered rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/4409—Non condensed pyridines; Hydrogenated derivatives thereof only substituted in position 4, e.g. isoniazid, iproniazid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/445—Non condensed piperidines, e.g. piperocaine
    • A61K31/4453—Non condensed piperidines, e.g. piperocaine only substituted in position 1, e.g. propipocaine, diperodon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Description

WO 00/35435 PCT/US99/29591 USE OF A MEK INHIBITOR FOR PREVENTING TRANSPLANT REJECTION FIELD OF THE INVENTION This invention relates to a method for preventing mammals that have undergone an organ, tissue, cell, or limb transplant from rejecting the transplant. 5 The method comprises administering an effective amount of a MEK inhibitor, ideally a phenyl amine derivative. BACKGROUND OF THE INVENTION Transplantation of organs and limbs has become a common procedure to treat mammals that have diseased organs, or have been the victims of accidents or 10 other traumas that have resulted in loss of organ function or limbs. Routinely transplanted organs include the liver, kidney, pancreas, and lung. Other types of transplantation are also common, such as skin, bone marrow, and small intestine. Limb transplantation includes fingers, toes, and larger limbs such as arms. Transplant rejection involves both humoral immunity and a cell-mediated 15 immune reaction, or a delayed type hypersensitivity response in a mammal patient. As a result, the patient receives an immunosuppressant agent to control or at least diminish the rejection response. While several immunosuppressants are currently available for clinical use, each is associated with adverse side effects. For example, cyclosporine is a cyclic peptide which inhibits the T-cell production 20 of several cytokines, including IL-2 (interleukin-2), IL-3, IL-4, IL-5, IFN-8, and probably other lymphokines. Cyclosporine is used extensively for the prophylaxis of organ rejection in allogeneic kidney, liver, and heart transplants. Cyclosporine is often used in combination with other immunosuppressant agents such as corticosteroids or azathioprine. Unfortunate side effects associated with 25 cyclosporine include nephrotoxicity, hepatotoxicity, severe renal dysfunction, tremor, hirsutism, and hypertension. Another immunosuppressive agent is mycophenolate mofetil, the 2-morpholinoethyl ester of mycophenolic acid that is frequently used by patients 1 WO 00/35435 PCT/US99/29591 receiving allogeneic renal transplants. This agent is often used in combination with other immunosuppressive agents, including cyclosporine and corticosteroids. Like cyclosporine, mycophenolate mofetil can cause side effects, most notably the increased risk of developing lymphomas and other malignancies, particularly 5 concerning the skin. Adverse effects on fetal development have also been noted. In view of the above, there is a continuing need for immunosuppressant agents not only useful for treating or preventing transplant rejection but also with less severe side effects than those associated with existing therapy. According to the present invention, compounds that are MEK inhibitors are useful for 10 preventing rejection of transplants in mammals. Moreover, these potent immunosuppressive agents may have fewer or no adverse side effects. The compounds to be administered according to this invention are described in US Patent No. 5,525,625, and in WO 98/37881, both of which are incorporated herein by reference. 15 SUMMARY OF THE INVENTION This invention provides a method for the prevention of rejection in a mammal of transplanted organs, tissues, and limbs, said method including administering an effective immunosuppressive amount of a selective MEK inhibitor to a subject who has undergone a transplant or is scheduled to undergo a 20 transplant. In a preferred embodiment, the MEK inhibitor administered is 2-(2-amino-3-methoxyphenyl)-4-oxo- 4 H-[l]benzopyran, also known as "98059", as described in US 5,525,625. In another preferred embodiment, the immunosuppressive agent administered is a phenyl amine compound of Formula I or II: R I N 25
-R
5 Br or R 3 2 WO 00/35435 PCT/US99/29591 In formula (I), Rl is hydrogen, hydroxy, C 1
-C
8 alkyl, CI-C 8 alkoxy, halo, trifluoromethyl, or CN. R 2 is hydrogen. R 3 , R 4 , and R 5 are independently selected from hydrogen, hydroxy, halo, trifluoromethyl, CI-C 8 alkyl, CI-C8 alkoxy, nitro, CN, and -(0 or NH)m-(CH2)n-R9.
R
9 is hydrogen, hydroxy, 5 COOH, or NR 10 RI 1; n is 0-4; m is 0 or 1. Each of R 1 0 and RI I is independently selected from hydrogen and Cl-C 8 alkyl, or taken together with the nitrogen to which they are attached can complete a 3-10 member cyclic ring optionally containing 1, 2, or 3 additional heteroatoms selected from 0, S, NH, or N-(CI-C8 alkyl). Z is COOR 7 , tetrazolyl, CONR 6
R
7 , CONHNRI 0 R1 1, or CH 2
OR
7 . 10 R 6 and R 7 independently are hydrogen, CI-C 8 alkyl, C 2
-C
8 alkenyl,
C
2
-C
8 alkynyl, (CO)-C 1
-C
8 alkyl, aryl, heteroaryl,
C
3
-C
10 cycloalkyl, or
C
3
-C
10 (cycloalkyl optionally containing one, two, or three heteroatoms selected from 0, S, NH, or N alkyl); or R 6 and R 7 together with the nitrogen to which they are attached complete a 3-10 member cyclic ring optionally containing 1, 2, or 3 15 additional heteroatoms selected from 0, S, NH, or N alkyl. In formula (I), any of the foregoing alkyl, alkenyl, aryl, heteroaryl, heterocyclic, and alkynyl groups can be unsubstituted or substituted by halo, hydroxy, CI-C 6 alkoxy, amino, nitro,
CI-C
4 alkylamino, di(Ci-C 4 )alkylamino, C 3
-C
6 cycloalkyl, phenyl, phenoxy, C 3
-C
5 heteroaryl, or C 3
-C
5 heteroaryloxy; or a pharmaceutically acceptable salt, ester, 20 amide, or prodrug thereof. Preferred embodiments of Formula (I) have a structure wherein: (a) R 1 is hydrogen, methyl, methoxy, fluoro, chloro, or bromo; (b) R 2 is hydrogen; (c) R 3 , R 4 , and R 5 independently are hydrogen, fluoro, chloro, bromo, iodo, methyl, methoxy, or nitro; (d) RIO and R 1 independently are hydrogen or methyl; 25 (e) Z is COOR 7 , tetrazolyl, CONR 6
R
7 , CONHNRiOR,,, or CH 2
OR
7 ; R 6 and R 7 independently are hydrogen, C 1-4 alkyl, heteroaryl, or C 3.5 cycloalkyl optionally containing one or two heteroatoms selected from 0, S, or NH; or R 6 and R 7 together with the nitrogen to which they are attached complete a 5-6 member cyclic ring optionally containing 1 or 2 additional heteroatoms selected from 0, 30 NH or N-alkyl; and wherein any of the foregoing alkyl or aryl groups can be unsubstituted or substituted by halo, hydroxy, methoxy, ethoxy, or heteroaryloxy 3 WO 00/35435 PCT/US99/29591 (such as the synthetic intermediate 2,3,4,5,6-pentafluorophenyl); (f) Z is COOR 7 ; (g) R 7 is H, pentafluorophenyl, or tetrazolyl; (h) R 3 , R4, and R 5 are independently H, fluoro, or chloro; (i) R 4 is fluoro; (j) two of R 3 , R4, and R 5 are fluoro; or (k) combinations of the above. In another preferred embodiment of Formula (I), R1 is 5 methyl, fluoro, chloro, or bromo. Examples of preferred embodiments include methods comprising a MEK inhibitor selected from Formula (I) Compound Table below. 4 WO 00/35435 PCT/US99/29591 FORMULA (I) COMPOUND TABLE (page 1 of 10) [4-Chloro-2-(1H-tetrazol-5-yl)-phenyl-(4-iodo-2-methyl-phenyl)-amine (4-iodo-2-methyl-phenyl)-[2-(1H-tetrazol-5-yl)-phenyl]amine 5 [4-nitro-2-(1H-tetrazol-5-yl)-phenyl-(4-iodo-2-methyl-phenyl)-amine 4-Fluoro-2-(4-iodo-2-methylphenylamino)-benzoic acid 3,4,5-Trifluoro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid 10 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid Sodium 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-benzoate 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-benzoic acid 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-benzoic acid 4-Chloro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid 15 2-(4-Iodo-2-methyl-phenylamino)-benzoic acid 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid 5-Iodo-2-(4-iodo-2-methyl-phenylamino)-benzoic acid 2,3,5-Trifluoro-4-(4-iodo-2-methyl-phenylamino)-benzoic acid 2-(4-Iodo-phenylamino)-5-methoxy-benzoic acid 20 5-Methyl-2-(4-iodo-2-methyl-phenylamino)-benzoic acid 2-(4-Iodo-2-methyl-phenylamino)-4-nitro-benzoic acid 2-(4-Bromo-2-methyl-phenylamino)-4-fluoro-benzoic acid 2-(2-Bromo-4-iodo-phenylamino)-5-nitro-benzoic acid 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-benzoic acid 25 5-Chloro-N-(2-hydroxyethyl)-2-(4-iodo-2-methyl-phenylamino) benzamide 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-methyl-benzamide N-Ethyl-4-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide 30 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-NN-dimethyl-benzamide 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(1H-tetrazol-5-yl) benzamide 5 WO 00/35435 PCT/US99/29591 FORMULA (I) COMPOUND TABLE (continued, page 2 of 10) 5 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-benzamide 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N,N-dimethyl-benzamide [5-Chloro-2-(4-iodo-2-methyl-phenylamino)-benzoylamino]-acetic acid 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-propyl-benzamide 5-Bromo-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino) 10 benzamide N,N-Diethyl-4-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide 4-Fluoro-N-{3-[4-(2-hydroxy-ethyl)-piperazin-1-yl]-propyl}-2-(4-iodo 2-methyl-phenylamino)-benzamide N,N-Diethyl-2-(4-iodo-2-methyl-phenylamino)-5-nitro-benzamide 15 N-Butyl-4-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide 5-Chloro-N,N-diethyl-2-(4-iodo-2-methyl-phenylamino)-benzamide 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N,N-dimethyl-benzamide 5-Bromo-3,4-difluoro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl phenylamino)-benzamide 20 N-(2,3-Dihydroxy-propyl)- 3 ,4-difluoro-2-(4-iodo-2-methyl-phenylamino) benzamide 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-piperidin-1 yl-ethyl)-benzamide 3,4-Difluoro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino) 25 benzamide N-(2,3-Dihydroxy-propyl)-4-fluoro-2-(4-iodo-2-methyl-phenylamino) benzamide 3,4-Difluoro-N-(3-hydroxy-propyl)-2-(4-iodo-2-methyl-phenylamino) benzamide 30 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyrrolidin 1 -yl-ethyl)-benzamide 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyridin-4 yl-ethyl)-benzamide 6 WO 00/35435 PCT/US99/29591 FORMULA (I) COMPOUND TABLE (continued, page 3 of 10) 4-Fluoro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino) 5 benzamide 5-Bromo-N-(3-dimethylamino-propyl)-3,4-difluoro-2-(4-iodo-2-methyl phenylamino)-benzamide 5-Bromo- 3 ,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-morpholin 4-yl-ethyl)-benzamide 10 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-morpholin-4-yl ethyl)-benzamide 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyrrolidin-1I-yl ethyl)-benzamide 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyridin-4-yl-ethyl) 15 benzamide N-(3-Dimethylamino-propyl)-3,4-difluoro-2-(4-iodo-2-methyl phenylamino)-benzamide N-Benzyl-4-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-(2-hydroxy-ethyl) 20 benzamide 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-morpholin-4-yl-ethyl) benzamide 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3-piperidin-1-yl-propyl) benzamide 25 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3-piperidin-1-yl propyl)-benzamide 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-thiophen-2-yl-ethyl) benzamide 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyrolidin-1 -yl-ethyl) 30 benzamide 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-(2-morpholin-4-yl ethyl)-benzamide 7 WO 00/35435 PCT/US99/29591 FORMULA (I) COMPOUND TABLE (continued, page 4 of 10) 5-Bromo-3
,
4 -difluoro-2-(4iodo2methy-phenyamino-Npyridin- 4 5 ylmethyl-benzamide 3
,
4 -Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-pyridin-4-ylmethyl benzamide 2-(4-Bromo-2-methyl-phenylamino)-N-(3-dimethylamino propyl)- 3 ,4-difluoro-benzamide 10 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-pyridin-4-ylmethyl benzamide 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyridin-4-yl-ethyl) benzamide 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-(2-pyridin-4-yl-ethyl) 15 benzamide 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-(3-hydroxy-propyl) benzamide 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-(2-pyrrolidin-1 -yl ethyl)-benzamide 20 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-phenethyl-benzamide 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-(2-thiophen-2-yl ethyl)-benzamide 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-pyridin-4-ylmethyl benzamide 25 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-phenethyl-benzamide 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-(2-piperidin-1-yl ethyl)-benzamide 5-Chloro-N-{3-[4-(2-hydroxy-ethyl)-piperazin-1-yl]-propyl}-2-(4-iodo-2 methyl- phenylamino)-benzamlide 30 5-Fluoro-N-{3-[4-(2-hydroxy-ethyl)-piperazin-1-yl]-propyl}-2-(4-iodo-2 methyl-phenylamino)- benzamide 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-N-pyridin-4-yl methyl benzamide 8 WO 00/35435 PCT/US99/29591 FORMULA (I) COMPOUND TABLE (continued, page 5 of 10) 5-Bromo-N-{3-[4-(2-hydroxy-ethyl)-piperazin-1-yl]-propyl}-2-(4-iodo-2 5 methyl- phenylamino)- benzamide 5-Chloro-N-(2-diethylamino-ethyl)-2-(4-iodo-2-methyl-phenylamino) benzamide 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-(2-piperidin- 1 -yl-ethyl) benzamide 10 (3-Hydroxy-pyrrolidin- 1 -yl)-[5-nitro-2-(4-iodo-2-methyl-phenylamino) phenyl]-methanone 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyolidin- 1 -yl-ethyl) benzamide 5-Bromo-N-(2-diethylamino-ethyl)-2-(4-iodo-2-methyl-phenylamino) 15 benzamide N- {2-[Bis-(2-hydroxy-ethyl)-amino]-ethyl} -5-chloro-2-(4-iodo-2-methyl phenylamino)- benzamide N- {2-[Bis-(2-hydroxy-ethyl)-amino]-ethyl} -5-bromo-2-(4-iodo-2-methyl phenylamino)- benzamide 20 N-{3-[4-(2-Hydroxy-ethyl)-piperazin-1-yl]-propyl}-2-(4-iodo-2-methyl phenylamino)- benzamide 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-pyridin-4-ylmethyl benzamide 5-Bromo-2-(4-iodo-2-ethyl-phenylamino)-N-(2-pyrrolidin- 1 -yl-ethyl) 25 benzamide 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(2-piperidin- 1 -yl-ethyl) benzamide 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyrrolidin- 1 -yl-ethyl) benzamide 30 5-Chloro-N-(3-dimethylamino-propyl)-2-(4-iodo-2-methyl-phenylamino) benzamide N- {2-[Bis-(2-hydroxy-ethyl)-amino]-ethyl} -5-fluoro-2-(4-iodo-2-methyl phenylamino)-benzamide 9 WO 00/35435 PCT[US99/29591 FORMULA (1) COMPOUND TABLE (continued, page 6 of 10) 5-Chloro-N-(3 -hydroxy-propy1)-2-(4-iodo-2-methy1-phelylailo) 5 benzamide 5-Chloro-N-(3 -diethylamnino-2-hydroxy-propy)-2-(4-iodo- 2 methyl phenylamino)- benzamnide 5-loo2(-oo2mty-peyaio--2pprdn1 -yl-ethyl) benzamnide 10 5-BooN(-yrx-rpl -(-oo2mty-hnlmn) benzamnide 5-Boo2(-od--ehlphnlmn)N-3pprn1 -yl-propyl) benzamnide N- 12- [Bis-(2-hydroxy-ethyl)-aminol -ethyl I -2-(4-iodo-2-methyl 15 phenylamnino)-5-nitro-benzamide 5-hoo2(-oo2mty-hnlaio--2mrhln4y-ty) benzamnide 5-Chloro-N-(3 -diethylamnino-propyl)-2-(4-iodo-2-methyl-phelylallfo) benzamnide 20 5-hooN(-ispoyaioehl--4id--ehlpeyann) benzamnide 5-hoo2(-oo2mty-hnlrio--3pprdn 1 -yl-propyl) benzamide 2-(4-Iodo-2-methyl-phenylamino)-5 -nitro-N-(2-piperidin- 1 -yl-ethyl) 25 benzamnide 5-rm--4id--ehlphnlrio--2pprzn 1 -yl-ethyl) benzamnide N-2Dehlmn-ty)5fur-2(-oo2mty-hnlmn) benzamide 30 5-Bromo-N-(3 -dimethylamino-propy1)-2-(4-iodo-2-methyl-phelylamilo) benzamnide N-3Hdoypoy)2(-oo2mty-hnlmn)5nto benzamnide 10 WO 00/35435 PCT/US99/29591 FORMULA (I) COMPOUND TABLE (continued, page 7 of 10) 5-Fluoro-N-(3-hydroxy-propyl)-2-(4-iodo-2-methyl-phenylamino) 5 benzamide N-(3-Diethylamino-propyl)-5-fluoro-2-(4-iodo-2-methyl-phenylamino) benzamide N-(3-Diethylamino-propyl)-2-(4-iodo-2-methyl-phenylamino)-5-nitro benzamide 10 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(2-morpholin-4-yl-ethyl) benzamide 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-N-(3-piperidin-1-yl-propyl) benzamide [5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-phenyl]-(2 or 3-hydroxy 15 pyrrolidin- 1 -yl)-methanone 5-Bromo-N-(2-diisopropylamino-ethyl)-2-(4-iodo-2-methyl-phenylamino) benzamide 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-morpholin-4-yl-ethyl) benzamide 20 5 -Fluoro-2-(4-iodo-2-methyl-phenylaino)-N-(3 -piperidin--yl-propyl) benzamide [5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-phenyl]-[4-(2-hydroxy-ethyl) piperazin- 1 -yl)-methanone N-(3-Diethylamino-2-hydroxy-propyl)-5-fluoro-2-(4-iodo-2-methyl 25 phenylamino)- benzamide N-Cyclopropyl-5-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide; 5-Chloro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino) benzamide 5-Fluoro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino) 30 benzamide N-Benzyloxy-5-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide N-Benzyloxy-5-bromo-2-(4-iodo-2-methyl-phenylamino)-benzamide 11 WO 00/35435 PCTIUS99/29591 FORMULA (I) COMPOUND TABLE (continued, page 8 of 10) 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-N-(4-sulfamoyl-benzyl) 5 benzamide 5-Bromo-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino) benzamide N-(2-Hydroxy-ethyl)-5-iodo-2-(4-iodo-2-methyl-phenylamino)-benzamide N-(2-Hydroxy-ethyl)-2-(4-iodo-2-ethyl-phenylamino)-5-nitro-benzamide 10 2-(4-Iodo-2-methyl-phenylamino)-N-methyl-5-nitro-N-phenyl-benzamide 5-Chloro-N-cyclopropyl-2-(4-iodo-2-methyl-phenylamino)-benzamide 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-methyl-N-phenyl benzamide N-Allyl-5-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide 15 N-Benzyloxy-5-iodo-2-(4-iodo-2-methyl-phenylamino)-benzamide 5-Fluoro-2-(4-iodo-2-methyl-phenylamilno)-N-(4-sulfamoyl-benzyl) benzamide N-Allyl-5-chloro-2-(4-iodo-2-methyl-phenylamino)-benzamide N-Cyclopropyl-2-(4-iodo-2-methyl-phenylamino)-5-nitro-benzamide 20 5-Bromo-N-cyclopropyl-2-(4-iodo-2-methyl-phenylamino)-benzamide 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-methyl-N-phenyl benzamide 5-Iodo-2-(4-iodo-2-methyl-phenylamino)-N-(4-sulfamoyl-benzyl) benzamide 25 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(4-sulfamoyl-benzyl) benzamide N-Allyl-2-(4-iodo-2-methyl-phenylamino)-5-nitro-benzamide 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-N-(4-sulfamoyl-benzyl) benzamide 30 N-Allyl-5-bromo-2-(4-iodo-2-methyl-phenylamino)-benzamide 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3-methyl-benzyl) benzamide N-Cyclopropyl-5-iodo-2-(4-iodo-2-methyl-phenylamino)-benzamide 12 WO 00/35435 PCT/US99/29591 FORMULA (I) COMPOUND TABLE (continued, page 9 of 10) 5-Bromo-2-(4-iodo-2-methyl-phenylamilno)-N-methyl-N-phenyl 5 benzamide N-Benzyloxy-2-(4-iodo-2-methyl-phenylamino)-5-nitro-benzamide N-Cyclohexyl-5-iodo-2-(4-iodo-2-methyl-phenylamino)-benzamide N-Allyl-5-iodo-2-(4-iodo-2-methyl-phenylamino)-benzamide 5-Iodo-2-(4-iodo-2-methyl-phenylamino)-N-(3-methyl-benzyl)-benzamide 10 2-(4-Iodo-2-methyl-phenylamino)-N-(3-methyl-benzyl)-5-nitro-benzamide 5-Iodo-2-(4-iodo-2-methyl-phenylamino)-N-methyl-N-phenyl-benzamide N-Cyclohexyl-5-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide 5-Chloro-N-cyclohexyl-2-(4-iodo-2-methyl-phenylamino)-benzamide 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(3-methyl-benzyl) 15 benzamide 5-Bromo-N-cyclohexyl-2-(4-iodo-2-methyl-phenylamino)-benzamide 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-(3-methyl-benzyl) benzamide N-Cyclohexyl-2-(4-iodo-2-methyl-phenylamino)-5-nitro-benzamide 20 N-Benzyloxy-5-bromo-2-(4-iodo-2-methyl-phenylamino)-benzamide N-Benzyloxy-5-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide 5-Chloro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino) benzamide 5-Bromo-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino) 25 benzamide 2-(4-Iodo-2-methyl-phenylamino)-N-methyl-5-nitro-N-phenyl-benzamide 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-methyl-N-phenyl benzamide N-(2-Hydroxy-ethyl)-5-iodo-2-(4-iodo-2-methyl-phenylamino)-benzamide 30 5-Chloro-N-cyclopropyl-2-(4-iodo-2-methyl-phenylamino)-benzamide N-Allyl-5-chloro-2-(4-iodo-2-methyl-phenylamino)-benzamide 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-methyl-N-phenyl benzamide 13 WO 00/35435 PCTIUS99/29591 FORMULA (I) COMPOUND TABLE (continued, page 10 of 10) N-(2-Hydroxy-ethyl)-2-(4-iodo-2-methyl-phelylamilo)-5-litro-beflzamide 5 5 -Fluoro-N-(2-hydroxy-ethy1)-2-(4-iodo-2-methyl-phelamilo) benzamide 5-Bromo-N-cyclopropy1-2-(4-iodo-2-methyl-pheflylamilo)-beflzamide N-Cyclopropy1-5-fluoro-2-(4-iodo-2-methy-phelamilo)-beflzamide 5 -Fluoro-2-(4-iodo-2-methyl-phenylaino)-N-(4-sulfamoyl-belzyl) 10 benzamide N-Cyclopropyl-2-(4-iodo-2-methy-phelylamilo)-5-litro-belzamlide N-Allyl-5-fluoro-2-(4-iodo-2-methyl-phenylamilo)-belzamide N-Benzyloxy-5-iodo-2-(4-iodo-2-methyl-phenylamilo)-belzamide N-A1y-5-bromo-2-(4-iodo-2-methyl-phenylamilo)-beflzamide 15 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(4-sulfamoyl-belzyl) benzamide 5-Bromo-2-(4-iodo-2-methy1-phenylamino)-N-methy1-N-pheflyl benzamide N-Allyl-2-(4-iodo-2-methyl-phenylaio)-5-litro-beflzamide 20 4-Fluoro-2-(4-iodo-2-methyl-phenylamilo)-belzyl alcohol [5-Chloro-2-(4-iodo-2-methyl-phenylamino)-phelyl] -methanol [2-(4-Iodo-2-methyl-phenylamino)-5 -nitro-phenyl] -methanll [5-Bromo-2-(4-iodo-2-methyl-phenylamino)-phelyl] -methanol N-Allyl-2-(4-iodo-2-methyl-phenylamilo)-5-litro-belzamfide. 25 14 WO 00/35435 PCT/US99/29591 In another preferred embodiment, the MEK inhibitor is a compound of Formula II Rl 2a C-N-0-R 7 a lia N 5a II I I I -R5a Br or I 3a R4a In Formula (II), Ria is hydrogen, hydroxy, C 1
-C
8 alkyl, Cl-C 8 alkoxy, 5 halo, trifluoromethyl, or CN. R2a is hydrogen. Each of R3a, R4a, and R5a is independently selected from hydrogen, hydroxy, halo, trifluoromethyl, Cl-C 8 alkyl, C 1
-C
8 alkoxy, nitro, CN, and (0 or NH)m-(CH2)n-R9a. R9a is hydrogen, hydroxy, CO 2 H or NR 1 0aRl 1 a; n is 0-4; and m is 0 or 1. Each of RlOa and R 1 1a is independently hydrogen or C 1
-C
8 alkyl, or taken together with 10 the nitrogen to which they are attached can complete a 3- to 10-member cyclic ring optionally containing one, two, or three additional heteroatoms selected from 0, S, NH, or N-(Ci-C 8 alkyl). R6a is hydrogen, Cl-C 8 alkyl, (CO)-(Cl-C 8 alkyl), aryl, aralkyl, or C 3 -ClO cycloalkyl. R7a is hydrogen, Cl-C 8 alkyl,
C
2
-C
8 alkenyl, C 2
-C
8 alkynyl, C 3
-C
1 0 (cycloalkyl or cycloalkyl optionally 15 containing a heteroatom selected from 0, S, or NR9a). In Formula (II), any of the alkyl, alkenyl, aryl, heterocyclic, and alkynyl groups can be unsubstituted or substituted by halo, hydroxy, CI-C 6 alkoxy, amino, nitro, CI-C 4 alkylamino, di(C 1 C 4 )alkylamino, C 3
-C
6 cycloalkyl, phenyl, phenoxy, C 3
-C
5 heteroaryl, or C3-Cs heteroaryloxy; or R6a and R7a taken together with the N to which they are 20 attached can complete a 5- to 10-membered cyclic ring, optionally containing one, two, or three additional heteroatoms selected from 0, S, or NR10aR11a. The invention also encompasses pharmaceutically acceptable salts, esters, amides or prodrugs of each of the disclosed compounds. Preferred embodiments of Formula (II) are those structures wherein: 15 WO 00/35435 PCT/US99/29591 (a) Ria is H, methyl, fluoro, or chloro; (b) R2a is H; R3a, R4a, and R5a are each H, Cl, nitro, or F; (c) R6a is H; (d) R 7 a is methyl, ethyl, 2-propenyl, propyl, butyl, pentyl, hexyl, cyclopropylmethyl, cyclobutylmethyl, cyclopropylmethyl, or cyclopropylethyl; (e) the 4' position is I, rather than Br; (f) R 4 a is F at the 4 5 position, para to the CO-N-R 6 a-OR 7 a group and meta to the bridging nitrogen; (f) R 3 a or R 5 a is F; (g) at least one of R 3 a, R 4 a, and R 5 a is F; (h) Ria is methyl or chloro; or (i) a combination of the above. In a more preferred embodiment the MEK inhibitor is a compound selected from Formula (II) Compound Table below. 16 WO 00/35435 PCT/US99/29591 FORMULA (II) COMPOUND TABLE (page 1 of 7) 4-Fluoro-N-hydroxy-2-(4-iodo-2-methyl-phenylamino)-benzamide 5 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(methoxy)-benzamide 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(prop-2-ynyloxy) benzamide 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-phenoxyethoxy) benzamide 10 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-thienylmethoxy) benzamide 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(prop-2-enyloxy) benzamide 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(cyclopropylmethoxy) 15 benzamide 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(cyclopentoxy)-benzamide 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3-furylmethoxy) benzamide 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-ethoxy-benzamide 20 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(but-2-enyloxy) benzamide 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(cyclopropylmethoxy) benzamide 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(1 -methylprop 25 2-ynyloxy)-benzamide 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3-phenylprop 2-ynyloxy)-benzamide 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3-methyl 5-phenylpent-2-en-4-ynyloxy)-benzamide 30 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(prop-2-ynyloxy) benzamide 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(propoxy)-benzamide 17 WO 00/35435 PCTIUS99/29591 FORMULA (11) COMPOUND TABLE (continued, page 2 of 7) 3 ,4-Difluoro-2-(4-iodo-2-methy-phenyaino)-N-(cyclobutyloxy) 5 benzamide 3 ,4-Difluoro-2-(4-iodo-2-methy-phenyamino)-N-(2-thielmethoxy) benzamide 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-methyl-prop 2-enyloxy)-benzamide 10 3 ,4-Difluoro-2-(4-iodo-2-methy-phelamilo)-N-(2-pheoxyethoxy) beuzamide 3 ,4-Difluoro-2-(4-iodo-2-methy-phenyamino)-N-(but-2-eloxy) benzamnide 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(but-3 -ynyloxy) 15 benzamnide 3 ,4-Difluoro-2-(4-iodo-2-methy-phenyaino)-N-(cyclopeltYoxy) benzamnide 3 ,4-Difluoro-2-(4-iodo-2-methy-phenyamino)-N-(3-(2-fluorophel) prop-2-ynyloxy)-benzamide 20 5-Bromo-3 ,4-difluoro-N-hydroxy-2-(4-iodo-2-methyl-phenylamilo) benzamnide 5-Bromo-3,4-difluoro-2-(4-iodo-2-methy-phelaifno)-N-(-propoxyV benzamnide 5-Bromo-3,4-difluoro-N-(fural-3-ylmethoxy)-2-(4-iodo-2-methylk 25 phenylamino)-benzamide 5-Bromo-N-(but-2-enyloxy)-3 ,4-difluoro-2-(4-iodo-2-methyl phenylamino)-benzamide 5-Bromo-N-butoxy-3 ,4-difluoro-2-(4-iodo-2-methyl-phenylamino) benzamide 30 5-Bromo-3 ,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3-methyl-but 2-enyloxy)-benzamnide 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamio)-N-(3-methyl pent-2-en-4-ynyloxy)-benzamnide 18 WO 00/35435 PCTIUS99/29591 FORMULA (11) COMPOUND TABLE (continued, page 3 of 7) 5-Bromo-3 ,4-difluoro-2-(4-iodo-2-methyl-benzyl)-N-[5-(3 -methoxy 5 phenyl)-3-methyl-pent,-2-en-4-ynyloxy] -benzamide 5-Bromo-3 ,4-difluoro-2-(4-iodo-2-methyl-phenylailo)-N-(prop 2-ynyloxy)-benzamnide 5-Bromo-3 ,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N [3-(3 -methoxy-phenyl)-prop-2-ynyloxy] -benzamide 10 5-Bromo-3 ,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(thiopefl 2-ylmethoxy)-benzamide 5-Bromo-3 ,4-difluoro-2-(4-iodo-2-methyl-phenylamnino)-N-(pyridifl 3-ylmethoxy)-benzamide 5-Bromo-3-4-difluoro-2-(4-iodo-2-methyl-phenylailo)-N 15 (3 -(2-fluorophenyl)-prop-2-ynyloxy)-benzamide 5-Bromo-3 ,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(ethoxy) benzamide 5-Bromo-3 ,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N (cyclopropylmethoxy)-benzamnide 20 5-Bromo-3 ,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(isopropoxy) benzamnide 5-Bromo-3 ,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N-but 3-ynyloxy)-benzamide 5-Chloro-N-hydroxy-2-(4-iodo-2-methy-phelamilo)-belzatfide 25 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-(tetrahydro-pyral-2-yloxy)> benzamnide 5-Chloro-2-(4-iodo-2-methy1-phenylamino)-N-methoxy-beflzamide 4-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-phelmethoxy-belzamide 4-Fluoro-2-(4-iodo-2-methy1-phenylamino)-N-phelylmethoxy-beflzamide 30 5-Fluoro-N-hydroxy-2-(4-iodo-2-methyl-phenylamilo)-beflzamide 5-Iodo-2-(4-iodo-2-methy1-phenylamino)-N-phenylmethoxy-benzamnide 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(tetrahydropyra-2-yloxy) benzamide 19 WO 00/35435 PCT/US99/29591 FORMULA (11) COMPOUND TABLE (continued, page 4 of 7) 3 ,4-Difluoro-2-(4-bromo-2-methy-phelamio)-N-(3-phellprop 5 2-ynyloxy)-benzamide 3 ,4-Difluoro-2-(4-bromo-2-methyl-phenylamilo)-N-(3 -furylmethoxy) benzamnide 3 ,4-Difluoro-2-(4-bromo-2-methy-phelamilo)-N-(2-thielmethoxy) benzamnide 10 3 ,4-Difluoro-2-(4-bromo-2-methy-phenyaio)-N-(but-3-yfloxy) benzamnide 3 ,4-Difluoro-2-(4-bromo-2-methy-phenyamilo)-N-(2-methy-prop 2-enyloxy)-benzamide 3 ,4-Difluoro-2-(4-bromo-2-methy-phenyamio)-N-(but-2-eloxy) 15 benzamnide 3 ,4-Difluoro-2-(4-bromo-2-methy-phelamilo)-N-(mfethoxy)-beflzamide 3 ,4-Difluoro-2-(4-bromo-2-methy-phenylamilo)-N-(ethoxy)-beflzamide 3 ,4-Difluoro-2-(4-bromo-2-methy-phenyamio)-N-(cyc1obutoxy) benzamnide 20 3 ,4-Difluoro-2-(4-bromo-2-methy-phenyamio)-N-(isopropoxy) benzamnide 3 ,4-Difluoro-2-(4-bromo-2-methy-phelamilo)-N-(2-pheoxyethoxy) benzamnide 3 ,4-Difluoro-2-(4-bromo-2-methy-phenyamio)-N-(cyclopropy[ 25 methoxy)-benzamide 3 ,4-Difluoro-2-(4-bromo-2-methy-phenyamio)-N-(l-propoxy) benzamnide 3 ,4-Difluoro-2-(4-bromo-2-methyl-phenylamilo)-N-( 1-methyl-prop 2-ynyloxy)-benzamide 30 3 ,4-Difluoro-2-(4-bromo-2-methyl-phelaio)-N-(3-(3-fluorophelyl) prop-2-ynyloxy)-benzamide 3 ,4-Difluoro-2-(4-bromo-2-methyl-phelaio)-N-(4,4-dimethy1peflt 2-ynyloxy)-benzamide 20 WO 00/35435 PCT/US99/29591 FORMULA (II) COMPOUND TABLE (continued, page 5 of 7) 3,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N-(cyclopentoxy) 5 benzamide 3,4,5-Trifluoro-N-hydroxy-2-(4-iodo-2-methyl-phenylamino)-benzamide 5-Chloro-3,4-difluoro-N-hydroxy-2-(4-iodo-2-methyl-phenylamilno) benzamide 5-Bromo-3,4-difluoro-2-(2-fluoro-4-iodo-phenylamino)-N-hydroxy 10 benzamide N-Hydroxy-2-(4-iodo-2-methyl-phenylamino)-4-nitro-benzamide 3,4,5-Trifluoro-2-(2-fluoro-4-iodo-phenylamino)-N-hydroxy-benzamide 5-Chloro-3,4-difluoro-2-(2-fluoro-4-iodo-phenylamino)-N-hydroxy benzamide 15 5-Bromo-2-(2-chloro-4-iodo-phenylamino)-3,4-difluoro-N-hydroxy benzamide 2-(2-Fluoro-4-iodo-phenylamino)-N-hydroxy-4-nitro-benzamide 2-(2-Chloro-4-iodo-phenylamino)-3,4,5-trifluoro-N-hydroxy-benzamide 5-Chloro-2-(2-chloro-4-iodo-phenylamino)-3,4-difluoro-N-hydroxy 20 benzamide 5-Bromo-2-(2-bromo-4-iodo-phenylamino)-3,4-difluoro-N-hydroxy benzamide 2-(2-Chloro-4-iodo-phenylamino)-N-hydroxy-4-methyl-benzamide 2-(2-Bromo-4-iodo-phenylamino)-3,4,5-trifluoro-N-hydroxy-benzamide 25 2-(2-Bromo-4-iodo-phenylamino)-5-chloro-3,4-difluoro-N-hydroxy benzamide 2-(2-Bromo-4-iodo-phenylamino)-N-hydroxy-4-nitro-benzamide 4-Fluoro-2-(2-fluoro-4-iodo-phenylamino)-N-hydroxy-benzamide 3,4-Difluoro-2-(2-fluoro-4-iodo-phenylamino)-N-hydroxy-benzamide 30 2-(2-Chloro-4-iodo-phenylamino)-4-fluoro-N-hydroxy-benzamide 2-(2-Chloro-4-iodo-phenylamino)-3,4-difluoro-N-hydroxy-benzamide 2-(2-Bromo-4-iodo-phenylamino)-4-fluoro-N-hydroxy-benzamide 2-(2-Bromo-4-iodo-phenylamino)-3,4-difluoro-N-hydroxy-benzamide 21 WO 00/35435 PCTfUS99/29591I FORMULA (11) COMPOUND TABLE (continued, page 6 of 7) N-Cyclopropylmethoxy-3 ,4,5-trifluoro-2-(4-iodo-2-methyl-phenylamino) 5 benzamnide 5-Chloro-N-cyclopropylmethoxy-3 ,4-difluoro-2-(4-iodo-2-methyl phenylamino)-benzamnide 5-Bromo-N-cyclopropylmethoxy-3 ,4-difluoro-2-(2-fluoro-4-iodo phenylamino)-benzamide 10 N-Cyclopropylmethoxy-2-(4-iodo-2-methyl-phelylaino)-4-flitro benzamnide N-Cyclopropylmethoxy-3 ,4,5-trifluoro-2-(2-fluoro-4-iodo-phenylamino) benzamnide 5 -Chloro-N-cyclopropylmethoxy-3 ,4-difluoro-2-(2-fluoro-4-iodo 15 phenylamino)-benzamide 5-Bromo-2.-(2-chloro-4-iodo-phenylamino)-N-cyclopropylmethoxy 3 ,4-difluoro-benzamnide N-Cyclopropylmethoxy-2-(2-fluoro-4-iodo-phenylamino)-4-litro benzamnide 20 2-(2-Chloro-4-iodo-phenylamino)-N-cyclopropylmethoxy-3 ,4,5-trifluoro benzamide 5-Chloro-2-(2-chloro-4-iodo-phenylamino)-N-cyclopropylmethoxy 3 ,4-difluoro-benzamide 5-Bromo-2-(2-bromo-4-iodo-phenylamino)-N-ethoxy-3,4-difluoro 25 benzamnide 2-(2-Chloro-4-iodo-phenylamino)-N-ethoxy-4-nitro-belzamide 2-(2-Bromo-4-iodo-phenylamino)-N-cyclopropylmethoxy-3 ,4,5-trifluoro benzamnide 2-(2-Bromo-4-iodo-phenylamino)-5-chloro-N-cycopropylmethoxy 30 3 ,4-difluoro-benzamide 2-(2-Bromo-4-iodo-phenylamino)-N-cyclopropylmethoxy-4-litro benzamnide 22 WO 00/35435 PCT/US99/29591 FORMULA (II) COMPOUND TABLE (continued, page 7 of 7) N-Cyclopropylmethoxy-4-fluoro-2-(2-fluoro-4-iodo-phenylamino) 5 benzamide N-Cyclopropylmethoxy-3,4-difluoro-2-(2-fluoro-4-iodo-phenylamino) benzamide 2-(2-Chloro-4-iodo-phenylamino)-N-cyclopropylmethoxy-4-fluoro benzamide 10 2-(2-Chloro-4-iodo-phenylamino)-N-cyclopropylmethoxy-3,4-difluoro benzamide 2-(2-Bromo-4-iodo-phenylamino)-N-cyclopropylmethoxy-4-fluoro benzamide 2-(2-Bromo-4-iodo-phenylamino)-N-cyclopropylmethoxy-3,4-difluoro 15 benzamide. In the most preferred embodiment of this invention, a compound selected from the following is administered to a patient (ie, a mammal) in an amount 20 that is effective to prevent or treat transplant rejection: 2-(2-Chloro-4-iodophenylamino)-N-cyclopropylmethoxy 3,4-difluorobenzamide (PD184352); 2-(2-Methyl-4-iodophenylamino)-N hydroxy-4-fluorobenzamide (PD 170611); 2-(2-Methyl-4-iodophenylamino)-N hydroxy-3,4-difluoro-5-bromobenzamide (PD171984); 2-(2-Methyl 25 4-iodophenylamino)-N-cyclopropylmethoxy-3,4-difluoro-5-bromobenzamide (PD177168); 2-(2-Methyl-4-iodophenylamino)-N-cyclobutylmethoxy 3,4-difluoro-5-bromobenzamide (PD 180841); 2-(2-Chloro 4-iodophenylamino)-N-cyclopropylmethoxy-3,4-difluoro-5-bromobenzamide (PD 184161); 2-(2-Chloro-4-iodophenylamino)-N-hydroxy-3,4-difluoro 30 5-bromobenzamide (PD184386); 2-(2-Chloro-4-iodophenylamino)-N cyclobutylmethoxy-3,4-difluorobenzamide (PD 185625); 2-(2-Chloro 4-iodophenylamino)-N-hydroxy-4-fluorobenzamide (PD 185848); 2-(2-Methyl-4-iodophenylamino)-N-hydroxy-3,4-difluorobenzamide (PD 188563); 2-(2-Methyl-4-iodophenylamino)-N-cyclopropylmethoxy 23 WO 00/35435 PCT/US99/29591 3,4,5-trifluorobenzamide (PD 198306); and 2-(2-Chloro-4-iodophenylamino) N-cyclopropylmethoxy-4-fluorobenzamide (PD 203311); and the benzoic acid derivatives thereof. For example, the benzoic acid derivative of PD 198306 is 2-(2-Methyl-4-iodophenylamino)-3,4,5-trifluorobenzoic acid. 5 Additional preferred compounds include 2-(2-chloro-4 iodophenylamino)-5-chloro-N-cyclopropylmethoxy -3,4-difluorobenzamide (PD 297189), 2-(4-iodophenylamino)-N-cyclopropylmethoxy-5-chloro-3,4 difluorobenzamide (PD 297190), 2-(4-iodophenylamino)-5-chloro-3,4 difluorobenzoic acid (PD 296771), 2-(2-chloro-4-iodophenylamino)-5-chloro 10 3,4-difluorobenzoic acid (PD 296770), 5-chloro-3,4-difluoro-2-(4-iodo-2 methylphenylamino)-benzoic acid (PD 296767); and 5-chloro-N cyclopropylmethoxy -3,4-difluoro-2-(4-iodo-2-methylphenylamino)-benzamide (PD 298127). Another preferred method according to this invention comprises 15 administering to a mammal that has undergone a transplant, or is about to undergo a transplant, the immunosuppressive agent which is 2-(2-chloro 4-iodophenylamino)-N-cyclopropylmethoxy-3,4-difluorobenzamide. Still another preferred method according to this invention employs the compound which is 2-(2-methyl-4-iodophenylamino)-N-cyclopropylmethoxy 20 3,4,5-trifluorobenzamide. The invention further provides methods of synthesis and synthetic intermediates. Other features and advantages of the invention are apparent from the detailed description, examples, and claims set forth. 25 BRIEF DESCRIPTION OF THE FIGURES Figure 1 shows the dose response ability of 2-(2-chloro-4 iodophenylamino)-N-cycloproplymethoxy-3,4-difluorobenzamide (PD 184352) to inhibit the cellular production of interleukin-2 (IL-2) in human peripheral blood mononuclear cells stimulated with concanavalin A (Con A). 24 WO 00/35435 PCT/US99/29591 Figure 2 shows the dose response ability of PD 184352 to inhibit the cellular production of IL-2 in human peripheral blood mononuclear cells stimulated with anti-CD3 plus anti-CD28. Figure 3 shows the dose response ability of PD 184352 to inhibit cellular 5 production of interferon-S (IFN-6) in cells stimulated with Con A. Figure 4 shows the ability of PD 184352 to suppress the human mixed lymphocyte reaction (MLR) as measured by the uptake of tritiated thymidine (3H-TDR). Figure 5 shows the dose response ability of PD 184352 to inhibit Con A 10 induced T-cell proliferation. Figure 6 shows the dose response ability of PD 184352 to inhibit T-cell proliferation induced by phytohemagglutinin (PHA). Figure 7 shows the lack of toxicity of PD 184352 in cells. Figure 8 shows the inhibitory activity of several MEK inhibitors against 15 MLR, IFN-gamma, and IL-2, and the ability of the compounds to inhibit PHA and Con A-induced proliferation with little or no toxicity (MTT). The compounds tested were PD 184352; 2-(2-methyl-4-iodophenylamino)-N-hydroxy-3,4-difluoro-5 bromobenzamide (PD 171984); 20 2-(2-methyl-4-iodophenylamino)-N-cyclopropylmethoxy-3,4-difluoro 5-bromobenzamide (PD 177168); 2-(2-chloro-4-iodophenylamino)-N-cycloproplymethoxy-3,4-difluoro 5-bromobenzamide (PD 184161); and 2-(2-chloro-4-iodophenylamino)-N-hydroxy-3,4-difluoro 25 5-bromobenzamide (PD 184386). Figure 9 shows the relative IL-2 suppressive activity of several phenyl amine compounds compared to rolipram and to dexamethasone (Dex). Figure 10 shows the comparative activity of several phenyl amines, rolipram, and dexamethasone to suppress production of IFN-S. 30 Figure 11 shows the human MLR suppressive activity of several phenyl amine MEK inhibitors compared to dexamethasone. Figure 12 shows the ability of several phenyl amine MEK inhibitors to suppress human T-cell proliferation, compared to dexamethasone. 25 WO 00/35435 PCT/US99/29591 Figure 13 shows the percent cell death caused by several phenyl amine MEK inhibitors in the human MTT test. DETAILED DESCRIPTION OF THE INVENTION This invention provides a method for the prophylaxis of rejection of 5 transplants in mammals, as well as control and maintenance of grafts. The invention is practiced by administering to a mammal that has undergone a transplant, or to a patient who is scheduled to undergo a transplant, an effective immunosuppressive amount of a selective MEK inhibitor to prevent or control the rejection of the transplanted organ, limb, cell(s), or tissue. For example, the 10 method is practiced by administering a phenyl amine MEK inhibitor that is described in WO 98/37881. These are selective MEK inhibitors, namely they inhibit MEK 1 and MEK 2 without substantial inhibition of other enzymes. The method is ideally suited to prevent and control of rejection of kidney, liver, lung, and limb transplants. 15 The mammals to be treated according to this invention are patients who have undergone a transplant of an organ, a tissue, a limb, or cells, or who are about to undergo such transplant. Those skilled in the medical art are readily able to identify individual patients who are in need of an immunosuppressive agent in order to prevent or control the rejection of a foreign organ, limb, cell, or tissue. 20 The compounds of the present invention are MEK inhibitors. A MEK inhibitor is a compound that shows MEK inhibition when tested in the assays titled "Enzyme Assays" in United States Patent Number 5,525,625, column 6, beginning at line 35. The complete disclosure of United States Patent Number 5,525,625 is hereby incorporated by reference. An example of a MEK inhibitor is 25 2-(2-amino-3-methoxyphenyl)-4-oxo-4H-[1]benzopyran. Specifically, a compound is a MEK inhibitor if a compound shows activity in the assay titled "Cascade Assay for Inhibitors of the MAP Kinase Pathway," column 6, line 36 to column 7, line 4 of the United States Patent Number 5,525,625 and/or shows activity in the assay titled "In Vitro MEK Assay" at column 7, lines 4 to 27 of the 30 above-referenced patent. 26 WO 00/35435 PCT/US99/29591 A. Terms Some of the terms used herein are defined below in combination with their 5 usage throughout this disclosure. As used herein, the term "aryl" means a cyclic, bicyclic, or tricyclic aromatic ring moiety having from five to twelve carbon atoms. Examples of typical aryl groups include phenyl, naphthyl, and fluorenyl. The aryl may be substituted by one, two, or three groups selected from fluoro, chloro, bromo, iodo, 10 alkyl, hydroxy, alkoxy, nitro, amino, alkylamino, or dialkylamino. Typical substituted aryl groups include 3-fluorophenyl, 3,5-dimethoxyphenyl, 4-nitronaphthyl, 2-methyl-4-chloro-7-aminofluorenyl, and the like. The term "aryloxy" means an aryl group bonded through an oxygen atom, for example phenoxy, 3-bromophenoxy, naphthyloxy, and 4-methyl 15 1 -fluorenyloxy. "Heteroaryl" means a cyclic, bicyclic, or tricyclic aromatic ring moiety having from four to eleven carbon atoms and one, two, or three heteroatoms selected from 0, S, or N. Examples include furyl, thienyl, pyrrolyl, pyrazolyl, imidazolyl, triazolyl, thiazolyl, oxazolyl, xanthenyl, pyronyl, indolyl, pyrimidyl, 20 naphthyridyl, pyridyl, benzinnidazolyl, and triazinyl. The heteroaryl groups can be unsubstituted or substituted by one, two, or three groups selected from fluoro, chloro, bromo, iodo, alkyl, hydroxy, alkoxy, nitro, amino, alkylamino, or dialkylamino. Examples of substituted heteroaryl groups include chloropyranyl, methylthienyl, fluoropyridyl, amino-1,4-benzisoxazinyl, nitroisoquinolinyl, and 25 hydroxyindolyl. The heteroaryl groups can be bonded through oxygen to make heteroaryloxy groups, for example thienyloxy, isothiazolyloxy, benzofuranyloxy, pyridyloxy, and 4-methylisoquinolinyloxy. The term "alkyl" means straight and branched chain aliphatic groups. 30 Typical alkyl groups include methyl, ethyl, isopropyl, tert.-butyl, 2,3-dimethylhexyl, and 1,1-dimethylpentyl. The alkyl groups can be unsubstituted or substituted by halo, hydroxy, alkoxy, amino, alkylamino, dialkylamino, cycloalkyl, aryl, aryloxy, heteroaryl, or heteroaryloxy, as those terms are defined 27 WO 00/35435 PCTIUS99/29591 herein. Typical substituted alkyl groups include chloromethyl, 3-hydroxypropyl, 2-dimethylaminobutyl, and 2-(hydroxymethylamino)ethyl. Examples of aryl and aryloxy substituted alkyl groups include phenylmethyl, 2-phenylethyl, 3-chlorophenylmethyl, 1,1-dimethyl-3-(2-nitrophenoxy)butyl, and 5 3,4,5-trifluoronaphthylmethyl. Examples of alkyl groups substituted by a heteroaryl or heteroaryloxy group include thienylmethyl, 2-furylethyl, 6-furyloxyoctyl, 4-methylquinolyloxymethyl, and 6-isothiazolylhexyl. Cycloalkyl substituted alkyl groups include cyclopropylmethyl, 2-cyclohexyethyl, piperidyl 2-methyl, 2-(piperidin-1-yl)-ethyl, 3-(morpholin-4-yl)propyl. 10 "Alkenyl" means a straight or branched carbon chain having one or more double bonds. Examples include but-2-enyl, 2-methyl-prop-2-enyl, 1,1-dimethyl hex-4-enyl, 3-ethyl-4-methyl-pent-2-enyl, and 3-isopropyl-pent-4-enyl. The alkenyl groups can be substituted with halo, hydroxy, alkoxy, amino, alkylamino, dialkylamino, aryl, aryloxy, heteroaryl, or heteroyloxy, for example 15 2-bromoethenyl, 3-hydroxy-2-butenyl, 1-aminoethenyl, 3-phenylprop-2-enyl, 6-thienyl-hex-2-enyl, 2-furyloxy-but-2-enyl, and 4-naphthyloxy-hex-2-enyl. "Alkynyl" means a straight or branched carbon chain having at least one triple bond. Typical alkynyl groups include prop-2-ynyl, 2-methyl-hex-5-ynyl, 3,4-dimethyl-hex-5-ynyl, and 2-ethyl-but-3-ynyl. The alkynyl groups can be 20 substituted as the alkyl and alkenyl groups, for example, by aryl, aryloxy, heteroaryl, or heteroaryloxy, for example 4-(2-fluorophenyl)-but-3-ynyl, 3-methyl-5-thienylpent-4-ynyl, 3-phenoxy-hex-4-ynyl, and 2-furyloxy-3-methyl hex-4-ynyl. The alkenyl and alkynyl groups can have one or more double bonds or 25 triple bonds, respectively, or a combination of double and triple bonds. For example, typical groups having both double and triple bonds include hex-2-en 4-ynyl, 3-methyl-5-phenylpent-2-en-4-ynyl, and 3-thienyloxy-hex-3-en-5-ynyl. The term "cycloalkyl" means a nonaromatic ring or fused rings. Examples include cyclopropyl, cyclobutyl, cyclopenyl, cyclooctyl, bicycloheptyl, adamantyl, 30 and cyclohexyl. The ring can optionally contain one, two, or three heteroatoms selected from 0, S, or N. Such groups include tetrahydrofiryl, tetrahydropyrrolyl, octahydrobenzofuranyl, morpholinyl, piperazinyl, pyrrolidinyl, piperidinyl, octahydroindolyl, and octahydrobenzothiofuranyl. The cycloalkyl groups can be 28 WO 00/35435 PCT/US99/29591 substituted with the same substituents as an alkyl and alkenyl groups, for example, halo, hydroxy, aryl, and heteroaryloxy. Examples include 3-hydroxycyclohexyl, 2-aminocyclopropyl, 2-phenylpyrrolidinyl, and 3-thienylmorpholin-1-yl. The term "maintenance" means controlling the tendency of a mammal to 5 reject a cell, organ, limb, or tissue that has been transplanted into or onto the mammals body. The method is practiced by administering an amount of a selective MEK inhibitor that is effective to prevent or control the rejection. The term "patient" means all animals including humans. Examples of patients include humans, cows, dogs, cats, goats, sheep, horses, and pigs. 10 Selective MEK 1 or MEK 2 inhibitors are those compounds which inhibit the MEK 1 or MEK 2 enzymes, respectively, without substantially inhibiting other enzymes such as MKK3, PKC, Cdk2A, phosphorylase kinase, EGF, and PDGF receptor kinases, and C-src. In general, a selective MEK 1 or MEK 2 inhibitor has an IC 50 for MEK 1 or MEK 2 that is at least one-fiftieth (1/50) that of 15 its IC 50 for one of the above-named other enzymes. Preferably, a selective inhibitor has an IC 50 that is at least 1/100, more preferably 1/500, and even more preferably 1/1000, 1/5000, or less than that of its IC 50 or one or more of the above named enzymes. 20 B. Administration and Formulation The MEK inhibitors of the present method can be administered to a patient as part of a pharmaceutically acceptable composition. The compositions can be administered to humans and animals either orally, rectally, parenterally (intravenously, intramuscularly, or subcutaneously), intracisternally, 25 intravaginally, intraperitoneally, intravesically, locally (powders, ointments, or drops), or as a buccal or nasal spray. Compositions suitable for parenteral injection may comprise physiologically acceptable sterile aqueous or nonaqueous solutions, dispersions, suspensions or emulsions, and sterile powders for reconstitution into sterile 30 injectable solutions or dispersions. Examples of suitable aqueous and nonaqueous carriers, diluents, solvents, or vehicles include water, ethanol, polyols (propyleneglycol, polyethyleneglycol, glycerol, and the like), suitable mixtures thereof, vegetable oils (such as olive oil), and injectable organic esters such as 29 WO 00/35435 PCT/US99/29591 ethyl oleate. Proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersions and by the use of surfactants. These compositions may also contain adjuvants such as preserving, 5 wetting, emulsifying, and dispensing agents. Prevention of the action of microorganisms can be ensured by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, and the like. It may also be desirable to include isotonic agents, for example sugars, sodium chloride, and the like. Prolonged absorption of the injectable pharmaceutical form can be brought 10 about by the use of agents delaying absorption, for example, aluminum monostearate and gelatin. Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active compound is admixed with at least one inert customary excipient (or carrier) such as sodium 15 citrate or dicalcium phosphate or (a) fillers or extenders, as for example, starches, lactose, sucrose, glucose, mannitol, and silicic acid, (b) binders, as for example, carboxymethylcellulose, alignates, gelatin, polyvinylpyrrolidone, sucrose, and acacia, (c) humectants, as for example, glycerol, (d) disintegrating agents, as for example, agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, 20 certain complex silicates, and sodium carbonate, (e) solution retarders, as for example paraffin, (f) absorption accelerators, as for example, quaternary ammonium compounds, (g) wetting agents, as for example, cetyl alcohol and glycerol monostearate, (h) adsorbents, as for example, kaolin and bentonite, and (i) lubricants, as for example, talc, calcium stearate, magnesium stearate, solid 25 polyethylene glycols, sodium lauryl sulfate, or mixtures thereof. In the case of capsules, tablets, and pills, the dosage forms may also comprise buffering agents. Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethyleneglycols, and the like. 30 Solid dosage forms such as tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells, such as enteric coatings and others well known in the art. They may contain opacifying agents, and can also be of such composition that they release the active compound or compounds in a certain part 30 WO 00/35435 PCT/US99/29591 of the intestinal tract in a delayed manner. Examples of embedding compositions which can be used are polymeric substances and waxes. The active compounds can also be in micro-encapsulated form, if appropriate, with one or more of the above-mentioned excipients. 5 Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, solutions, suspensions, syrups, and elixirs. In addition to the active compounds, the liquid dosage forms may contain inert diluents commonly used in the art, such as water or other solvents, solubilizing agents and emulsifiers, as for example, ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, 10 benzyl alcohol, benzyl benzoate, propyleneglycol, 1,3-butyleneglycol, dimethylformamide, oils, in particular, cottonseed oil, groundnut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol, tetrahydrofurfuryl alcohol, polyethyleneglycols, and fatty acid esters of sorbitan or mixtures of these substances, and the like. 15 Besides such inert diluents, the composition can also include adjuvants, such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents. Suspensions, in addition to the active compounds, may contain suspending agents, as for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol 20 and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar and tragacanth, or mixtures of these substances, and the like. Compositions for rectal administrations are preferably suppositories which can be prepared by mixing the compounds of the present invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethyleneglycol, or a 25 suppository wax, which are solid at ordinary temperatures but liquid at body temperature and therefore, melt in the rectum or vaginal cavity and release the active component. Dosage forms for topical administration of a compound of this invention include ointments, powders, sprays, and inhalants. The active component is 30 admixed under sterile conditions with a physiologically acceptable carrier and any preservatives, buffers, or propellants as may be required. Ophthalamic formulations, eye ointments, powders, and solutions are also contemplated as being within the scope of this invention. 31 WO 00/35435 PCT/US99/29591 The compounds of the present method can be administered to a patient at dosage levels in the range of about 0.1 to about 1000 mg per day. For a normal human adult having a body weight of about 70 kg, a dosage in the range of about 0.01 to about 100 mg per kg of body weight per day is preferable. The specific 5 dosage used, however, can vary. For example, the dosage can depend on a numbers of factors including the requirements of the patient, the severity of the condition being treated, and the pharmacological activity of the compound being used. The determination of optimum dosages for a particular patient is well known to those skilled in the art. 10 The compounds of the present method can be administered as pharmaceutically acceptable salts, esters, amides, or prodrugs. The term "pharmaceutically acceptable salts, esters, amides, and prodrugs" as used herein refers to those carboxylate salts, amino acid addition salts, esters, amides, and prodrugs of the compounds of the present invention which are, within the scope of 15 sound medical judgment, suitable for contact with the tissues of patients without undue toxicity, irritation, allergic response, and the like, commensurate with a reasonable benefit/risk ratio, and effective for their intended use, as well as the zwitterionic forms, where possible, of the compounds of the invention. The term "salts" refers to the relatively non-toxic, inorganic and organic acid addition salts 20 of compounds of the present invention. These salts can be prepared in situ during the final isolation and purification of the compounds or by separately reacting the purified compound in its free base form with a suitable organic or inorganic acid and isolating the salt thus formed. Representative salts include the hydrobromide, hydrochloride, sulfate, bisulfate, nitrate, acetate, oxalate, valerate, oleate, 25 palmitate, stearate, laurate, borate, benzoate, lactate, phosphate, tosylate, citrate, maleate, fumarate, succinate, tartrate, naphthylate, mesylate, glucoheptonate, lactiobionate and laurylsulphonate salts, and the like. These may include cations based on the alkali and alkaline earth metals, such as sodium, lithium, potassium, calcium, magnesium and the like, as well as nontoxic ammonium, quaternary 30 ammonium, and amine cations including, but not limited to ammonium, tetramethylammonium, tetraethylammonium, methylamine, dimethylamine, trimethylamine, triethylamine, ethylamine, and the like. (See, for example, S.M. Berge, et al., "Pharmaceutical Salts," J Pharm. Sci., 1977;66:1-19 which is 32 WO 00/35435 PCT/US99/29591 incorporated herein by reference.) Examples of pharmaceutically acceptable, non-toxic esters of the compounds of this invention include Cl-C 6 alkyl esters wherein the alkyl group is a straight or branched chain. Acceptable esters also include C 5
-C
7 cycloalkyl 5 esters as well as arylalkyl esters such as, but not limited to benzyl. C 1
-C
4 alkyl esters are preferred. Esters of the compounds of the present invention may be prepared according to conventional methods. Examples of pharmaceutically acceptable, non-toxic amides of the compounds of this invention include amides derived from ammonia, primary 10 CI-C 6 alkyl amines and secondary CI-C 6 dialkyl amines wherein the alkyl groups are straight or branched chain. In the case of secondary amines the amine may also be in the form of a 5 or 6 membered heterocycle containing one nitrogen atom. Amides derived from ammonia, C 1
-C
3 alkyl primary amines and Cl-C 2 dialkyl secondary amines are preferred. Amides of the compounds of the 15 invention may be prepared according to conventional methods. The term "prodrug" refers to compounds that are rapidly transformed in vivo to yield the parent compound of the above formula, for example, by hydrolysis in blood. A thorough discussion is provided in T. Higuchi and V. Stella, "Pro-drugs as Novel Delivery Systems," Vol. 14 of the A.C.S. 20 Symposium Series, and in Bioreversible Carriers in Drug Design, ed. Edward B. Roche, American Pharmaceutical Association and Pergamon Press, 1987, both of which are incorporated herein by reference. In addition, the compounds of the present method can exist in unsolvated as well as solvated forms with pharmaceutically acceptable solvents such as water, 25 ethanol, and the like. In general, the solvated forms are considered equivalent to the unsolvated forms for the purposes of the present invention. Some of the compounds of the present method can exist in different stereoisometric forms by virtue of the presence of chiral centers. It is contemplated that all stereoisometric forms of the compounds as well as mixtures 30 thereof, including racemic mixtures, form part of this invention. 33 WO 00/35435 PCT/US99/29591 C. Synthesis The examples presented below are intended to illustrate particular embodiments of the invention and are not intended to limit the scope of the specification, including the claims, in any way. After the priority date of the 5 present disclosure, related syntheses and MEK inhibition data were also published in WO 99/01421 and WO 99/01426, hereby incorporated by reference. The 2-(4-bromo and 4-iodo phenylamino)-benzoic acid derivatives of Formula (I ) can be prepared from commercially available starting materials utilizing synthetic methodologies well-known to those skilled in organic 10 chemistry. A typical synthesis is carried out by reacting a 4-bromo or 4-iodo aniline with a benzoic acid having a leaving group at the 2-position to give a 2-(phenylamino)-benzoic acid. This process is depicted in Scheme 1. Scheme 1 0 11 2 C-OH NH L + R5 Br or I R3 R4 base 0 || N C-OH Br or I 15 where L is a leaving group, for example halo such as fluoro. The reaction of aniline and the benzoic acid derivative generally is accomplished by mixing the benzoic acid with an equimolar quantity or excess of 34 WO 00/35435 PCT/US99/29591 the aniline in an unreactive organic solvent such as tetrahydrofuran or toluene, in the presence of a base such as lithium diisopropylamide, n-butyl lithium, sodium hydride, triethylamine, and Hunig's base. The reaction generally is carried out at a temperature of about -78'C to about 100*C, and normally is complete within 5 about 2 hours to about 4 days. The product can be isolated by removing the solvent, for example by evaporation under reduced pressure, and further purified, if desired, by standard methods such as chromatography, crystallization, or distillation. The 2-(phenylamino)-benzoic acid (e.g., Formula I, where R 7 is hydrogen) 10 can be reacted with an organic or inorganic base such as pyridine, triethylamine, calcium carbonate, or sodium hydroxide to produce a pharmaceutically acceptable salt. The free acids can also be reacted with an alcohol of the formula HOR 7 (where R 7 is other than hydrogen, for example methyl) to produce the corresponding ester. Reaction of the benzoic acid with an alcohol can be carried 15 out in the presence of a coupling agent. Typical coupling reagents include 2-ethoxy- 1 -ethoxycarbonyl-1,2-dihydroquinoline (EEDQ), 1,3-dicyclohexylcarbodiimide (DCC), bromo-tris(pyrrolidino)- phosphonium hexafluorophosphate (PyBrOP), and (benzotriazolyloxy) tripyrrolidino phosphonium hexafluorophosphate (PyBOP). The phenylamino benzoic acid and 20 alcohol derivative normally are mixed in approximately equimolar quantities in an unreactive organic solvent such as dichloromethane, tetrahydrofiran, chloroform, or xylene, and an equimolar quantity of the coupling reagent is added. A base such as triethylamine or diisopropylethylamine can be added to act as an acid scavenger if desired. The coupling reaction generally is complete after about 10 minutes to 25 2 hours, and the product is readily isolated by removing the reaction solvent, for instance by evaporation under reduced pressure, and purifying the product by standard methods such as chromatography or crystallizations from solvents such as acetone, diethyl ether, or ethanol. The benzamides of the invention, Formula (I )where Z is CONR 6
R
7 , are 30 readily prepared by reacting the foregoing benzoic acids with an amine of the formula HNR 6
R
7 . The reaction is carried out by reacting approximately equimolar quantities of the benzoic acid and amine in an unreactive organic 35 WO 00/35435 PCTIUS99/29591 solvent in the presence of a coupling reagent. Typical solvents are chloroform, dichloromethane, tetrahydrofuran, benzene, toluene, and xylene. Typical coupling reagents include DCC, EEDQ, PyBrOP, and PyBOP. The reaction is generally complete after about 10 minutes to about 2 hours when carried out at a 5 temperature of about 0*C to about 60*C. The product amide is readily isolated by removing the reaction solvent, for instance by evaporation, and further purification can be accomplished by normal methods such as chromatography, crystallization, or distillation. The hydrazides (z = CONHNR 1 0 RI 1 ) are similarly prepared by coupling a benzoic acid with a hydrazine of the formula 10 H 2 HNRiORi1. The benzyl alcohols of the invention, compounds of Formula (I) where Z is CH 2 OR6 and R 6 is hydrogen, are readily prepared by reduction of the corresponding benzoic acid according to the following Scheme 2. 15 Scheme 2 0 -O2H2OH RI Ii RI r2 N reducing N I - ~R 5 agent _ I I T R Br or I Br or I Typical reducing agents commonly employed include borane in tetrahydrofuran. The reduction normally is carried out in an unreactive organic solvent such as tetrahydrofuran, and generally is complete within about 2 hours to about 24 hours 20 when conducted at a temperature of about 0*C to about 40*C. The following detailed examples illustrate specific compounds provided by this invention. 36 WO 00/35435 PCT/US99/29591 EXAMPLE 1 4-Fluoro-2-(4-iodo-2-methylphenylamino)benzoic acid To a stirring solution comprised of 3.16 g (0.0133 mol) of 2-amino-5 iodotoluene in 5 mL of tetrahydrofuran at -78*C was added 10 mL (0.020 mol) of 5 a 2.0 M lithium diisopropylamide in tetrahydrofuran/heptane/ethenylbenzene (Aldrich) solution. The resulting green suspension was stirred vigorously for 15 minutes, after which time a solution of 1.00 g (0.00632 mol) of 2,4-difluorobenzoic acid in 10 mL of tetrahydrofuran was added. The reaction temperature was allowed to increase slowly to room temperature, at which 10 temperature it was stirred for 2 days. The reaction mixture was concentrated. Aqueous HCl (10%) was added to the concentrate, and the solution was extracted with dichloromethane. The organic phase was dried (MgSO4) and then boiled over a steambath to low volume and cooled to room temperature. The off-white fibers were collected by vacuum filtration, rinsed with hexanes, and vacuum-oven 15 dried. (76*C; ca. 10 mm of Hg) to afford 1.10 g (47%) of the desired material; mp 224-229.5*C; 1 H NMR (400 MHz; DMSO): 8 9.72 (s, 1H), 7.97 (dd, 1H, J = 7.0, 8.7 Hz), 7.70 (d, 1H, J = 1.5 Hz), 7.57 (dd, 1H, J = 8.4, 1.9 Hz), 7.17 (d, 1H, J = 8.2 Hz), 6.61-6.53 (in, 2H), 2.18 (s, 3H); 20 13 C NMR (100 MHz; DMSO): 8 169.87, 167.60, 165.12, 150.17, 150.05, 139.83, 138.49, 136.07, 135.31, 135.20, 135.07, 125.60, 109.32, 105.09, 104.87, 99.72, 99.46, 89.43, 17.52; 19 F NMR (376 MHz; DMSO): 8 -104.00 to -104.07 (in); IR (KBr) 1670 (C = 0 stretch) cm- 1 ; 25 MS (CI) M+1 = 372. Analysis calculated for C 1 4
H
11 FIN0 2 : C, 45.31; H, 2.99; N, 3.77. Found: C, 45.21; H, 2.77; N, 3.64. EXAMPLES 2-30 30 By following the general procedure of Example 1, the following benzoic acids and salts of Formula (I) were prepared. 37 WO 00/35435 PCT/US99/29591 Example Compound MP 0 C No. 2 3,4,5-Trifluoro-2-(4-iodo-2-methyl-phenylamino)- 206-210 benzoic acid 3 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-benzoic 240.5-244.5 acid 4 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl- 259.5-262 phenylamino)-benzoic acid 5 5-Chloro-2-(2-chloro-4-iodo-phenylamino)-benzoic acid 255-260 6 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid 234-238 7 Sodium 5-Chloro-2-(4-iodo-2-methyl-phenylamino)- 310-320 DEC benzoate 8 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-benzoic acid 239.5-240 9 2-(2-Chloro-4-iodo-phenylamino)-5-nitro-benzoic acid 289-293 10 4-Fluoro-2-(3-fluoro-4-iodo-2-methyl-phenylamino)- 233-235 benzoic acid 11 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-benzoic acid 264-267 12 2-(2-Fluoro-4-iodo-phenylamino)-5-nitro-benzoic acid 256-258 13 2-(4-Bromo-2-methyl-phenylamino)-4-fluoro-benzoic 218.5-220 acid 14 2-(2-Bromo-4-iodo-phenylamino)-5-nitro-benzoic acid 285-288 DEC 15 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro- 230-234 benzoic acid 16 3-Fluoro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid 218-221 17 3,4-Difluoro-2-(4-iodo-2-methoxy-phenylamino)- 230-233 benzoic acid 18 4-Chloro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid 245-255 DEC 38 WO 00/35435 PCTIUS99/29591 Example Compound MP OC No. 19 2-(4-Iodo-2-methyl-phenylamino)-benzoic acid 218-223 20 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid 243-46 21 5-Iodo-2-(4-iodo-2-methyl-phenylamino)-benzoic acid 241-245 22 2,3,5-Trifluoro-4-(4-iodo-2-methyl-phenylamino)- 218-222 benzoic acid 23 4-Fluoro-2-(3-chloro-4-iodo-2-methyl-phenylamino)- 248-252.5 benzoic acid 24 2-(4-Iodo-phenylamino)-5-methoxy-benzoic acid 208-211 25 3-Chloro-2-(2-chloro-4-iodo-phenylamino)-benzoic acid 232-233 26 2-Fluoro-6-(4-iodo-2-methyl-phenylamino)-benzoic acid 179-182 27 4-Fluoro2-(2,3-dimethyl-4-iodo-2-methyl- 258-261 phenylamino)benzoic acid 28 5-Methyl-2-(4-iodo-2-methyl-phenylamino)-benzoic 209.5-211 acid 29 2-Chloro-6-(4-iodo-2-methyl-phenylamino)-benzoic acid 171-175 30 2-(4-Iodo-2-methyl-phenylamino)-4-nitro-benzoic acid 251-263 EXAMPLE 31 5-Chloro-N-(2-hydroxvethyl)-2-(4-iodo-2-methyl-phenylamino)-benzamide To a stirring solution comprised of 0.1020 g (0.2632 mmol) of 5-chloro 2-(4-iodo-2-methyl-phenylamino)-benzoic acid, 0.1 mL (1.7 mmol) of 5 ethanolamine, and 0.05 mL (0.29 mmol) of diisopropylethylamine in 5 mL of a 1:1 (v/v) tetrahydrofuran-dichloromethane solution was added 0.15 g (0.29 mmol) of solid PyBOP powder directly. The reaction mixture was stirred at room temperature overnight. The solvent was removed in vacuo. The crude residue was partitioned between ether (50 mL) and 10% aqueous hydrochloric acid (50 mL). 10 The organic phase was washed with 10% aqueous sodium hydroxide (50 mL), dried (MgSO4) and concentrated in vacuo to afford a yellow-brown oil which was crystallized from hexanes-ether to afford 0.0831 g (73%) of a green-yellow powder; mp 120-121*C; 39 WO 00/35435 PCTIUS99/29591 1 H NMR (400 MHz; CDCl 3 ): 6 9.11 (s, 1H), 7.56 (d, 1H, J = 1.4 Hz), 7.46-7.41 (m, 2H), 7.20 (dd, 1H, J = 8.9, 2.4 Hz), 7.00 (t, 2H, J = 9.6 Hz), 6.55 (broad t, 1H), 3.86 (t, 2H, J= 5.0 Hz), 3.61 (dd, 2H, J = 10.1, 5.5 Hz), 2.23 (s, 3H), 1.56 (broad s, 1H); 5 IR (KBr) 3297 (0-H stretch), 1627 (C = 0 stretch) cm- 1 ; MS (CI) M+1 =431. Analysis calculated for C 16
H
16
CIIN
2 0 2 : C, 44.62; H, 3.74; N, 6.50. Found: 44.63; H, 3.67; N, 6.30. 10 EXAMPLES 32-48 By following the general procedure of Example 31, the following benzamides were prepared by reacting the corresponding benzoic acid with the corresponding amine. Example Compound MP *C No. 32 4-Methoxy-N-(4-methoxy-phenyl)-3-nitro- 153.5-156 benzamide 33 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)- 158 benzamide 34 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N- 102.5-104.5 methyl-benzamide 35 N-Ethyl-4-fluoro-2-(4-iodo-2-methyl-phenylamino)- 90-91 benzamide 36 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N,N- oil dimethyl-benzamide 37 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(1H- 285-288 DEC tetrazol-5-yl)-benzamide 38 5-Bromo-2-(4-iodo-2-methyl-phenylamino)- 180-182 benzamide 39 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N,N- 137-138 dimethyl-benzamide 40 WO 00/35435 PCT/US99/29591 Example Compound MP *C No. 40 [5-Chloro-2-(4-iodo-2-methyl-phenylamino)- 170-173 benzoylamino]-acetic acid 41 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N- 69-71 propyl-benzamide 42 5-Bromo-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl- 132-133.4 phenylamino)-benzamide 43 N,N-Diethyl-4-fluoro-2-(4-iodo-2-methyl- oil phenylamino)-benzamide 44 4-Fluoro-N-{3-[4-(2-hydroxy-ethyl)-piperazin-1-yl]- 122-124 propyl}-2-(4-iodo-2-methyl-phenylamino) benzamide 45 N,N-Diethyl-2-(4-iodo-2-methyl-phenylamino)-5- 91-93 nitro-benzamide 46 N-Butyl-4-fluoro-2-(4-iodo-2-methyl-phenylamino)- 97-99 benzamide 47 5-Chloro-NN-diethyl-2-(4-iodo-2-methyl- 118-120 phenylamino)-benzamide 48 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-NN- 142.5-144 dimethyl-benzamide EXAMPLE 49 4-Fluoro-2-(4-iodo-2-methyl-phenvlamino)-benzyl alcohol 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid (0.50 g, 1.35 mmol) was dissolved in 6 mL (6 mmol) of cold 1.0 M borane 5 tetrahydrofuran complex in tetrahydrofuran solution. The reaction mixture was stirred under nitrogen atmosphere at room temperature overnight. The reaction was quenched with 80 mL of methanol. Concentration in vacuo produced a clear tan oil which was purified by MPLC. Elution with dichloromethane afforded 0.4285 g (89%) of a white solid; mp 99-100.5*C; 10 1 H NMR (400 MHz; DMSO): 6 7.57 (d, 1H, J=1.7 Hz), 7.45 (dd, 1H, J=8.4, 1.9 Hz), 7.39 (s, 1H), 7.29 (t, 1H, J=7.5 Hz), 6.89 (d, 1H, J=8.4 Hz), 6.67-6.60 (m, 1H), 5.47 (t, 1H, J=5.5 Hz), 4.49 (d, 2H, 5.1 Hz), 2.14 (s, 3H); 41 WO 00/35435 PCT/US99/29591 IR (KBr) 3372 (0-H stretch) cm- 1 ; MS (CI) M+1 = 358. Analysis calculated for C 14
H
13 FINO: C, 47.08; H, 3.67; N, 3.92. 5 Found: C, 47.17; H, 3.75; N, 3.72. EXAMPLE 50-52 The following benzyl alcohols were prepared by the general procedure of Example 49. Example No. Compound MP *C 50 [5-Chloro-2-(4-iodo-2-methyl-phenylamino)- 82-85 phenyl]-methanol 51 [2-(4-Iodo-2-methyl-phenylamino)-5-nitro-phenyl]- 126.5-128.5 methanol 52 [5-Bromo-2-(4-iodo-2-methyl-phenylamino)- 60.5-63.5 phenyl]-methanol Several invention compounds of Formula (I) were prepared utilizing 10 combinatorial synthetic techniques. The general procedure is as follows: To a 0.8-mL autosampler vial in a metal block was added 40 pL of a 0.5 M solution of the acid in DMF and 40 p.L of the reagent amine (2 M solution in Hunig's base and 1 M in amine in DMF). A 0.5 M solution of PyBrop was freshly prepared and 50 pL were added to the autosampler vial. The reaction was 15 allowed to stand for 24 hours. The reaction mixture was transferred to a 2-dram vial and diluted with 2 mL of ethyl acetate. The organic layer was washed with 3 mL of distilled water and the water layer washed again with 2 mL of ethyl acetate. The combined organic layers were allowed to evaporate to dryness in an open fume hood. 20 The residue was taken up in 2 mL of 50% acetonitrile in water and injected on a semi-prep reversed phase column (10 mm x 25 cm, 5 pM spherical silica, pore size 115 A derivatized with C- 18, the sample was eluted at 4.7 mL/min with 42 WO 00/35435 PCT/US99/29591 a linear ramp to 100% acetonitrile over 8.5 minutes. Elution with 100% acetonitrile continued for 8 minutes). Fractions were collected by monitoring at 214 nM. The residue was dissolved in chloroform and transferred to a preweighed vial, evaporated, and weighed again to determine the yield. 5 EXAMPLES 53-206 The following compounds of Formula I were prepared by combinatorial methodology: Example Compound MS No. M-H 53 5-Bromo-3,4-difluoro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl- 510 phenylamino)-benzamide 54 N-(2,3-Dihydroxy-propyl)-3,4-difluoro-2-(4-iodo-2-methyl- 462 phenylamino)-benzamide 55 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2- 577 piperidin- I -yl-ethyl)-benzamide 56 3,4-Difluoro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl- 432 phenylamino)-benzamide 57 N-(2,3-Dihydroxy-propyl)-4-fluoro-2-(4-iodo-2-methyl- 444 phenylamino)-benzamide 58 3,4-Difluoro-N-(3-hydroxy-propyl)-2-(4-iodo-2-methyl- 446 phenylamino)-benzamide 59 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N- 564 (2-pyrrolidin- I -yl-ethyl)-benzamide 60 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N- 571 (2-pyridin-4-yl-ethyl)-benzamide 61 4-Fluoro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino)- 414 benzamide 62 5-Bromo-N-(3-dimethylamino-propyl)-3,4-difluoro-2-(4-iodo- 551 2-methyl-phenylamino)-benzamide 43 WO 00/35435 PCTIUS99/29591 Example Compound MIS No. M-H 63 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamilo)-N- 580 (2-morpholin-4-yl-ethyl)-benzamide 64 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-morpholin- 501 4-yl-ethyl)-benzamide 65 3,4-Difluoro-2-(4-iodo-2-methyl-pbenylamino)-N-(2-pyrrolidin- 485 1 -yl-ethyl)-benzamide 66 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyridil-4-yI- 493 ethyl)-benzamide 67 N-(3-Dimethylamino-propyl)-3,4-difluoro-2-(4-iodo-2-methyl- 473 phenylamino)-benzamide 68 N-Benzyl-4-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide 460 69 2-(4-Bromo-2-methyl-phenylamino)-3 ,4-difluoro-N-(2-hydroxy- 384 ethyl)-benzamide 70 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-morpholin-4-yl- 483 ethyl)-benzamide 71 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3 -piperidin- Il-yI- 495 propyl)-benzamide 72 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3-piperidin- 513 1 -yl-propyl)-benzamide 73 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-thiophen-2-yI- 480 ethyl)-benzamide 74 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyrrolidin- l-yl- 467 ethyl)-benzamide 75 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-(2-morpholin- 453 4-yl-etbyl)-benzamide 76 5-Bromo-3 ,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N- 557 pyridin-4-ylmethyl-benzamide 77 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-pyridin- 479 4-ylmethyl-benzamide 78 2-(4-Bromo-2-methyl-phenylamino)-N-(3-dimethylamino-propyl)- 425 3,4-difluoro-benzamide 44 WO 00/35435 PCT/US99/29591 Example Compound MS No. M-H 79 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-pyridin-4-ylmethyl- 461 benzamide 80 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyridin-4-yl- 475 ethyl)-benzamide 81 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-(2-pyridin- 445 4-yl-ethyl)-benzamide 82 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-(3-hydroxy- 400 propyl)-benzamide 83 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-(2-pyrrolidin- 437 1 -yl-ethyl)-benzamide 84 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-phenethyl- 474 benzamide 85 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-(2-thiophen- 450 2-yl-ethyl)-benzamide 86 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-pyridin- 431 4-ylmethyl-benzamide 87 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-phenethyl- 444 benzamide 88 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-(2-piperidin- 451 1 -yl-ethyl)-benzamide 89 5-Chloro-N- {3-[4-(2-hydroxy-ethyl)-piperazin- I -yl]-propyl} - 557* 2-(4-iodo-2-methyl- phenylamino)- benzamide 90 5-Fluoro-N- {3 -[4-(2-hydroxy-ethyl)-piperazin- 1 -yl]-propyl}- 541* 2-(4-iodo-2-methyl- phenylamino)- benzamide 91 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-N-pyridin-4-yl methyl- 487 benzamide 92 5-Bromo-N-{3-[4-(2-hydroxy-ethyl)-piperazin-1-yl]-propyl}- 601* 2-(4-iodo-2-methyl- phenylamino)- benzamide 93 5-Chloro-N-(2-diethylamino-ethyl)-2-(4-iodo-2-methyl- 486* phenylamino)- benzamide 45 WO 00/35435 PCT/US99/29591 Example Compound MS No. M-H 94 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-(2-piperidin- 1-yl- 497* ethyl)-benzamide 95 (3-Hydroxy-pyrrolidin- 1 -yl)-[2-(4-iodo-2-methyl-phenylamino)- 466 5-nitro-phenyl]-methanone 96 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyrrolidin- l-yl- 484* ethyl)-benzamide 97 5-Bromo-N-(2-diethylamino-ethyl)-2-(4-iodo-2-methyl- 530* phenylamino)- benzamide 98 N-{2-[Bis-(2-hydroxy-ethyl)-amino]-ethyl}-5-chloro-2-(4-iodo- 518* 2-methyl- phenylamino)- benzamide 99 N-{2-[Bis-(2-hydroxy-ethyl)-amino]-ethyl}-5-bromo-2-(4-iodo- 562* 2-methyl- phenylamino)- benzamide 100 [5-Bromo-2-(4-iodo-2-methyl-phenylamino)-phenyl]-(3-hydroxy- 499 pyrrolidin- I -yl)-methanone 101 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-benzoic acid phenethyl 501 ester 102 N-{3-[4-(2-Hydroxy-ethyl)-piperazin-I -yl]-propyl}-2-(4-iodo- 568* 2-methyl-phenylamino)- benzamide 103 [5-Chloro-2-(4-iodo-2-methyl-phenylamino)-phenyl]-(3-hydroxy- 455 pyrrolidin- I -yl)-methanone 104 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-pyridin-4-ylmethyl- 460 benzamide 105 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyrrolidin-1-yl- 528* ethyl)-benzamide 106 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(2-piperidin-l-yl- 542* ethyl)-benzamide 107 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyrrolidin-1-yl- 468* ethyl)-benzamide 108 5-Chloro-N-(3-dimethylamino-propyl)-2-(4-iodo-2-methyl- 472* phenylamino)-benzamide 109 N-{2-[Bis-(2-hydroxy-ethyl)-amino]-ethyl}-5-fluoro-2-(4-iodo- 502* 2-methyl- phenylamino)- benzamide 46 WO 00/35435 PCT/US99/29591 Example Compound MS No. M-H 110 5-Chloro-N-(3-hydroxy-propyl)-2-(4-iodo-2-methyl- 445* phenylamino)-benzamide 111 5-Chloro-N-(3-dietbylamino-2-hydroxy-propyl)-2-(4-iodo- 516* 2-methyl-phenylamino)- benzamide 112 5-Fluoro-2-(4-iodo-2-methyl-phenylamiflo)-N-(2-piperidifl- Il-yl- 482* ethyl)-benzamide 113 5-Bromo-N-(3 -hydroxy-propyl)-2-(4-iodo-2-methyl- 489* phenylamino)-benzamide 114 5-Bromo-2-(4-iodo-2-methyl-pbenylamino)-N-(3 -piperidin- Il-yl- 556* propyl)-benzamide 115 N- {2-IIBis-(2-bydroxy-ethyl)-amino]-ethyl I -2-(4-iodo-2-methyl- 529* phenylamino)-5-nitro- benzamide 116 5-Chloro-2-(4-iodo-2-methyl-phelylamilo)-N-(2-morpholil-4-yl- 500* ethyl)-benzamide 117 5-Chloro-N-(3-diethylamino-propyl)-2-(4-iodo-2-methyl- 500* phenylamino)-benzamide 118 5-Chloro-N-(2-diisopropylamino-ethyl)-2-(4-iodo-2-methy1- 514* phenylamino)-benzamide 119 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-(3-piperidifl- -yl- 512* propyl)-benzamide 120 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-N-(2-piperidil- l-yl- 509* ethyl)-benzamide 121 5-Bromo-2-(4-iodo-2-methyl-phelamiflo)-N-(2-piperazil- l-yl- 544* etbyl)-benzamide 122 N-(2-Diethylamino-ethyl)-5-fluoro-2-(4-iodo-2-methyl- 470* phenylamino)-benzamide 123 5-Bromo-N-(3-dimethylamino-propyl)-2-(4-iodo-2-methyl- 516* phenylamino)-benzamide 124 N-(3-Hydroxy-propyl)-2-(4-iodo-2-methyl-phelylamilo)-5-litro- 456* benzamide 125 5-Fluoro-N-(3-hydroxy-propyl)-2-(4-iodo-2-methyl- 429* phenylamino)-benzamide 47 WO 00/35435 PCTIUS99/29591 Example Compound MS No. M-H 126 N-(3-Diethylamino-propyl)-5-fluoro-2-(4-iodo-2-methyl- 484* phenylamino)-benzamide 127 N-(3-Diethylamino-propyl)-2-(4-iodo-2-methyl-phenylamino)- 511* 5-nitro-benzamide 128 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(2-morpholin-4-yl- 544* ethyl)-benzamide 129 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-N-(3-piperidin-l-yl- 523* propyl)-benzamide 130 [5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-phenyl]-(3-hydroxy- 439 pyrrolidin- I -yl)-methanone 131 5-Bromo-N-(2-diisopropylamino-ethyl)-2-(4-iodo-2-methyl- 558* phenylamino)-benzamide 132 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-morpholin-4-yl- 484* ethyl)-benzamide 133 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3-piperidin-l-yl- 496* propyl)-benzamide 134 [5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-phenyl]- 482 [4-(2-hydroxy-ethyl)-piperazin- 1 135 N-(3-Diethylamino-2-hydroxy-propyl)-5-fluoro-2-(4-iodo- 500* 2-methyl-phenylamino)- benzamide 136 [5-Chloro-2-(4-iodo-2-methyl-phenylamino)-benzoylamino]- 443 acetic acid 137 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-N-(2-pyrrolidin- I-yl- 495* ethyl)-benzamide 138 N-(3-Dimethylamino-propyl)-2-(4-iodo-2-methyl-phenylamino)- 483* 5-nitro-benzamide 139 N-(2-Diisopropylamino-ethyl)-5-fluoro-2-(4-iodo-2-methyl- 498* phenylamino)- benzamide 140 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-thiobenzoic acid S- 490 phenethyl ester 141 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-thiobenzoic acid S- 506 phenethyl ester 48 WO 00/35435 PCTIUS99/29591 Example Compound MS No. M-H 142 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-thiobenzoic acid S- 536 benzyl ester 143 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-thiobenzoic acid S- 503 benzyl ester 144 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-thiobenzoic acid S- 476 benzyl ester 145 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-thiobenzoic acid S- 492 benzyl ester 146 N-Cyclopropyl-5-fluoro-2-(4-iodo-2-methyl-phenylamino)- 409 benzamide 147 5-Chloro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino)- 429 benzamide 148 5-Fluoro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino)- 413 benzamide 149 N-Benzyloxy-5-fluoro-2-(4-iodo-2-methyl-phenylamino)- 475 benzamide 150 N-Benzyloxy-5-bromo-2-(4-iodo-2-methyl-phenylamino)- 593* benzamide 151 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-N-(4-sulfamoyl- 567 benzyl)-benzamide 152 5-Bromo-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino)- 473 benzamide 153 N-(2-Hydroxy-ethyl)-5-iodo-2-(4-iodo-2-methyl-phenylamino)- 521 benzamide 49 WO 00/35435 PCT[US99/29591 Example Compound MS No. M-H 154 N-(2-Hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino)-5-litro- 440 benzamide 155 2-(4-Iodo-2-methyl-phenylamino)-N-methyl-5-nitro-N-phelyl- 486 benzamide 156 5-Chloro-N-cyclopropyl-2-(4-iodo-2-methyl-phenylamilo)- 425 benzamide 157 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-methyl-N-phel- 459 benzamide 158 N-Allyl-5-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide 409 159 N-Benzyloxy-5-iodo-2-(4-iodo-2-methyl-phenylamilo)- 583 benzamide 160 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(4-sulfamoyl- 538 benzyl)-benzamide 161 N-Allyl-5-chloro-2-(4-iodo-2-methyl-phenylamino)-belzamlide 425 162 N-Cyclopropyl-2-(4-iodo-2-methyl-phenylamilo)-5 -itro- 436 benzamide 163 5-Bromo-N-cyclopropyl-2-(4-iodo-2-methyl-phelylamilo)- 469 benzamide 164 5-Chloro-2-(4-iodo-2-metbyl-phenylamino)-N-methyl-N-phelyl- 475 benzamide 165 5-Iodo-2-(4-iodo-2-methyl-phenylamino)-N-(4-sulfamoy-belzyl)- 646 benzamide. 166 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(4-sulfamoyl- 598 benzyl)-benzamide 167 N-Allyl-2-(4-iodo-2-methyl-phenylamino)-5-nitro-benzamlide 436 50 WO 00/35435 PCT/US99/2959 I Example Compound MS No. M-H 168 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-N-(4-sulfamoyI- 565 benzyl)-benzamide 169 N-Allyl-5-bromo-2-(4-iodo-2-methyl-phenylamino)-belzamide 469 170 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3-methYl-belzyl)- 473 benzamide 171 N-Cyclopropyl-5-iodo-2-(4-iodo-2-metbyl-phenylamilo)- 517 benzamide 172 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-methyl-N-phelyl- 519 benzamide 173 N-Benzyloxy-2-(4-iodo-2-methyl-phenylamino)-5 -itro- 502 benzamide, 174 N-Cyclohexyl-5-iodo-2-(4-iodo-2-methyl-phenylamilo)-59 benzamide 175 N-Allyl-5-iodo-2-(4-iodo-2-methyl-phenylamino)-belzamide 517 176 5-lodo-2-(4-iodo-2-methyl-phenylamino)-N-(3-methyl-belzyl)- 581 benzamide 177 2-(4-Iodo-2-methyl-phenylamino)-N-(3-metbyl-benzyl)-5-litro- 500 benzamide 178 5-Iodo-2-(4-iodo-2-methyl-phenylamino)-N-methyl-N-phelyl- 567 benzamide 179 N-Cyclohexyl-5-fluoro-2-(4-iodo-2-methyl-phenylamilo)- 451 benzamide 180 5-Chloro-N-cyclohexyl-2-(4-iodo-2-methyl-phelylamilo)- 467 benzamide 181 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(3-methyl-belzyl)- 533 benzamide 182 5-Bromo-N-cyclohexyl-2-(4-iodo-2-methyl-phelylalilo)- 511 benzamide 183 5-Chloro-2-(4-iodo-2-methyl-phelylamilo)-N-(3-methyl-belzyl)- 489 benzamide 184 N-Cyclohexyl-2-(4-iodo-2-methyl-phenylamilo)-5 -itro- 478 benzamide 51 WO 00/35435 PCTIUS99/29591 Example Compound MIS No. M-H 185 N-Benzyloxy-5-bromo-2-(4-iodo-2-methyl-phenylamino)- 538 benzamide 186 N-Benzyloxy-5-fluoro-2-(4-iodo-2-niethyl-phenylamilo)- 477 benzamide 187 5-Chloro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phelylamilo)- 431 benzamide 188 5-Bromo-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phelylamilo)- 475 benzamide 189 2-(4-Iodo-2-methyl-phenylamino)-N-methyl-5-nitro-N-phelyl- 488 benzamide 190 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-methyl-N-phenyl- 477 benzamide 191 N-(2-Hydroxy-ethyl)-5-iodo-2-(4-iodo-2-methyl-phenylamino)- 523 benzamide 192 5-Chloro-N-cyclopropyl-2-(4-iodo-2-methyl-phenylamino)- 425 benzamide 193 N-Allyl-5-chloro-2-(4-iodo-2-methyl-phenylamilo)-belzamide 427 194 5-Fluoro-2-(4-iodo-2-methyl-pbenylamino)-N-methyl-N-phelyl- 461 benzamide 195 N-(2-Hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino)-5-nitro- 442 benzamide 196 5-Fluoro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phelylamilo)- 415 benzamide 197 5-Bromo-N-cyclopropyl-2-(4-iodo-2-methyl-phenylamino)- 472 benzamide, 198 N-Cyclopropyl-5-fluoro-2-(4-iodo-2-methyl-phelylamilo)- 411 benzamide 199 5-Fluoro-2-(4-iodo-2-methyl-phenylamilo)-N-(4-sulfamoyl- 540 benzyl)-benzamide 200 N-Cyciopropyl-2-(4-iodo-2-methyl-phenylamino)-5-litro- 438 benzamide 201 N-Allyl-5-fluoro-2-(4-iodo-2-methyl-phelylamilo)-belzamide 411 52 WO 00/35435 PCT/US99/29591 Example Compound MS No. M-H 202 N-Benzyloxy-5-iodo-2-(4-iodo-2-methyl-phenylamino)- 585 benzamide 203 N-Allyl-5-bromo-2-(4-iodo-2-methyl-phenylamino)-benzamide 472 204 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(4-sulfamoyl- 601 benzyl)-benzamide 205 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-methyl-N-phenyl- 522 benzamide 206 N-Allyl-2-(4-iodo-2-methyl-phenylamino)-5-nitro-benzamide 438 *M+H EXAMPLE 207 Preparation of [4-Chloro-2-(1H-tetrazol-5-yl)-(4-iodo-2-methyl-phenvl)-amine Step a: Preparation of 5-chloro-2-fluoro-benzaldehyde To a solution of 1-chloro-4-fluorobenzne (13.06 g, 0.1 mol) in THF 5 (180 mL), at -78*C, LDA (2M solution in THF, 50 mL, 0.1 mol) was added drop wise. After stirring at -78*C for 1.5 hours, DMF (8 mL) was added to the reaction mixture and allowed to warm up to room temperature overnight. The reaction mixture was partitioned between water and Et 2 0. The Et 2 0 layer was dried (MgSO4) and the solvent removed in vacuum to give 14.95 g (94%) yield of 10 crude aldehyde: 1 H NMR (CDCl 3 ): 5, 10.3 (s, -C(=0)H). Step b: Preparation of 5-chloro-2-fluoro-benzaldehyde oxime A solution of 5-chloro-2-fluoro-benzaldehyde (10 g, 0.0631 mol), hydroxylamine hydrochloride (6.57 g, 0.0946 mol) and pyridine (8.3 mL, 0.1010 mol) in EtOH (100 mL) was heated at 75*C (oil bath temperature) for 15 1 hour and the solvent removed under vacuum to give an oil. The oil was partitioned between water and CH 2 Cl 2 . The CH 2 Cl 2 layer was dried (MgSO 4 ) and the solvent removed under vacuum to give crude aldoxime as a solid. The 53 WO 00/35435 PCTIUS99/29591 solid was purified by medium pressure liquid chromatography on silica. Elution with CH 2 Cl 2 gave 4.87 g (28%) of the aldoxime as white solid: mp 95-97*C; Analysis calculated for C 7
H
5 NOFCl: C, 48.44; H, 2.90; N, 8.07. 5 Found: C, 48.55; H, 2.69, N, 7.90. Step c: Preparation of 5-chloro-2-fluoro-benzonirile A solution of the 5-chloro-2-fluoro-benzaldehyde oxime (3.15 g, 0.0182 mol) in acetic anhydride (150 mL) was refluxed for 16 hours. The reaction mixture was cooled to room temperature and poured into saturated aqueous 10 NaHCO3 (200 mL) solution. The mixture was extracted with Et 2 0. The Et 2 0 layer was dried (K 2
CO
3 ) and the solvent removed to give the product as an oily solid. The product was used without further purification in the next step. Step d: Preparation of 5-(5-chloro-2-fluoro-phenyl)-1H-tetrazole A mixture of 5-chloro-2-fluoro-benzonitrile (2.84 g, 0.01823 mol), butanol 15 (15 mL), sodium azide (1.543 g, 0.0237 mol), acetic acid (1.36 mL, 0.0237 mol) was refluxed for 24 hours. The reaction mixture was cooled to room temperature, additional 1.543 g sodium azide added, and the reaction mixture refluxed for additional 24 hours. After cooling to room temperature, Et 2 0 (100 mL) and 10% aqueous NaOH (200 mL) were added sequentially. The mixture was vigorously 20 stirred. The aqueous layer was separated, cooled with ice-methanol bath (-15*C) and acidified to pH 1 with conc. HCl. A gray solid precipitated. The solid was dried in vacuum at 50*C to give 1.76 g (49%) of 5-(5-chloro-2-fluoro-phenyl)- 1 H tetrazole: mp partial melt at 1 10*C, complete melting at 124*C); 1 H (400 Mz, CDCl 3 ): 8 8.19-8.08 (in, 1H), 7.77-7.71 (in, 1H), 7.61-7.52 (m, 1H); 25 13 C (100 Mz, CDCl 3 ): 8 159.00, 156.49, 140.88, 133.02, 132.93, 130.73, 129.23, 129.21, 129.08, 126.05, 118.96, 118.73, 114.50; MS (CI) M+1 = 199 (100), M = 198 (6). 54 WO 00/35435 PCT/US99/29591 Step e: Preparation of [4-Chloro-2-(1H-tetrazol-5-yl)-(4-iodo-2-methyl-phenyl) amine To a solution of 2-methyl-4-iodoaniline (3.52 g, 0.0151 mol) in THF (25 mL) at -78*C, LDA (2 molar solution in THF, 11.33 mL, 0.02267 mol) was 5 added dropwise. After stirring for 0.5 hours, a solution of 1-(tetrazol-5-yl)-2 fluoro-5-chlorobenzene (1.5 g, 0.00756 mol) in THF (15 mL) was added dropwise. The reaction was stirred for 16 hours as it warmed up to room temperature. The reaction mixture was quenched with aqueous conc. NH 4 Cl solution and extracted with CH 2 Cl 2 . The organic layer was dried (MgSO4) and 10 the solvent removed giving a crude product as an oil. The oil with CH 2 Cl 2 >CH 2 Cl 2 :MeOH (9.7:0.3) gave 1.5 g (48%) of the desired product: mp 205-208*C; 1 H (400 Mz, DMSO): 8 9.13 (s, 1H), 8.00-7.99 (s, 1H), 7.69 (s, 1H), 7.55-7.52 (m, 1H), 7.43-7.40 (m, 1H), 7.12-7.05 (m, 11), 2.24 (s, 3H); 1 3 C (100 Mz, CDCl 3 ): 6 141.87, 139.28, 138.88, 135.47, 133.71, 131.65, 128.15, 15 123.69, 121.94, 116.68, 87.79, 17.22; MS (CI) M+2 = 413 (44), M+1 = 412 (85), M = 411 (100). Analysis calculated for C 14 HI IN 5 CII-0.5H 2 0: C, 39.97; H, 2.87; N, 16.65. Found: C, 38.87, H, 2.77; N, 16.47. 20 The following tetrazole substituted phenylamines were prepared by following the general procedure of Example 207. EXAMPLE 208 (4-iodo-2-methyl-phenvl)-r2-(1H-tetrazol-5-vl)-phenvllamine, mp 231*C (dec) EXAMPLE 209 25 r4-nitro-2-(1H-tetrazol-5-yl)-(4-iodo-2-methyl-phenvl)-amine, mp 205-2081C. The 4-bromo and 4-iodo phenylamino benzhydroxamic acid derivatives of Formula II can be prepared from commercially available starting materials utilizing synthetic methodologies well-known to those skilled in organic 55 WO 00/35435 PCTIUS99/29591 chemistry. A typical synthesis is carried out by reacting a 4-bromo or 4-iodo aniline with a benzoic acid having a leaving group at the 2-position to give a phenylamino benzoic acid, and then reacting the benzoic acid phenylamino derivative with a hydroxylamine derivative (Scheme 3). 5 Scheme 3 0 j(2a C-OH Ria NH L + I R5a Br or I R3a R4a base 0 2a C-OH a N R5a Br or I R3a R4a 6a 1HN-0 - R7a R2a C-N-0-R 7 a Ria N I R5a Br or I R3a R4a 56 WO 00/35435 PCT/US99/29591 where L is a leaving group, for example halo such as fluoro, chloro, bromo or iodo, or an activated hydroxy group such as a diethylphosphate, trimethylsilyloxy, p-nitrophenoxy, or phenylsulfonoxy. The reaction of aniline and the benzoic acid derivative generally is 5 accomplished by mixing the benzoic acid with an equimolar quantity or excess of the aniline in an unreactive organic solvent such as tetrahydrofuran, or toluene, in the presence of a base such as lithium diisopropylamide, n-butyl lithium, sodium hydride, and sodium amide. The reaction generally is carried out at a temperature of about -78*C to about 25*C, and normally is complete within about 2 hours to 10 about 4 days. The product can be isolated by removing the solvent, for example by evaporation under reduced pressure, and further purified, if desired, by standard methods such as chromatography, crystallization, or distillation. The phenylamino benzoic acid next is reacted with a hydroxylamine derivative HNR6aOR7a in the presence of a peptide coupling reagent. 15 Hydroxylamine derivatives that can be employed include methoxylamine, N-ethyl-isopropoxy amine, and tetrahydro-oxazine. Typical coupling reagents include 2-ethoxy- 1 -ethoxycarbonyl-1,2-dihydroquinoline (EEDQ), 1,3-dicyclohexylcarbodiimide (DCC), bromo-tris(pyrrolidino)-phosphonium hexafluorophosphate (PyBrOP) and (benzotriazolyloxy)tripyrrolidino 20 phosphonium hexafluorophosphate (PyBOP). The phenylamino benzoic acid and hydroxylamino derivative normally are mixed in approximately equimolar quantities in an unreactive organic solvent such as dichloromethane, tetrahydrofuran, chloroform, or xylene, and an equimolar quantity of the coupling reagent is added. A base such as triethylamine or diisopropylethylamine can be 25 added to act as an acid scavenger if desired. The coupling reaction generally is complete after about 10 minutes to 2 hours, and the product is readily isolated by removing the reaction solvent, for instance by evaporation under reduced pressure, and purifying the product by standard methods such as chromatography or crystallizations from solvents such as acetone, diethyl ether, or ethanol. 30 An alternative method for making the invention compounds involves first converting a benzoic acid to a hydroxamic acid derivative, and then reacting the 57 WO 00/35435 PCT/US99/29591 hydroxamic acid derivative with an aniline. This synthetic sequence is depicted in Scheme 4. Scheme 4 0 0 R II 6a C-OH f6a L C-N-0-R 7 a L HN-0-R 7 a x b R3a R4a R3a R4a
NHR
2 Rla Br or I 0 6a R la 2a C-N-O-R 7 a N
-
I R5a Br or I R3a R4a 5 where L is a leaving group. The general reaction conditions for both of the steps in Scheme 4 are the same as those described above for Scheme 3. Yet another method for making invention compounds comprises reacting a phenylamino benzhydroxamic acid with an ester forming group as depicted in Scheme 5. 58 WO 00/35435 PCT/US99/29591 Scheme 5 ~i6a j2a C-N-OH lia N I R 5 a + L - R 7 a Br or I R3a R4a base 0R 1 6a R 2a C-N-O- R 7 a Ria N 1 -R5a Br or I R3a R4a where L is a leaving group such as halo, and a base is triethylamine or diisopropylamine. 5 The synthesis of compounds of Formula (II) is further illustrated by the following detailed examples. EXAMPLE la 4-Fluoro-N-hydroxy-2-(4-iodo-2-methyl-phenylamino)-benzamide (a) Preparation of 4-Fluoro-2-(4-iodo-2-methyl-phenvlamino)-benzoic acid 10 To a stirred solution containing 3.16 g (0.0133 mol) of 2-amino 5-iodotoluene in 5 mL of tetrahydrofuran at -78*C was added 10 mL (0.020 mol) of a 2.0 M lithium diisopropylamide in tetrahydrofuran/heptane/ethylbenzene (Aldrich) solution. The resulting green suspension was stirred vigorously for 15 minutes, after which time a solution of 1.00 g (0.00632 mol) of 15 2,4-difluorobenzoic acid in 10 mL of tetrahydrofuran was added. The reaction temperature was allowed to increase slowly to room temperature, at which 59 WO 00/35435 PCTIUS99/29591 temperature the mixture was stirred for 2 days. The reaction mixture was concentrated by evaporation of the solvent under reduced pressure. Aqueous HCl (10%) was added to the concentrate, and the solution was extracted with dichloromethane. The organic phase was dried (MgSO 4 ) and then concentrated 5 over a steambath to low volume (10 mL) and cooled to room temperature. The off-white fibers which formed were collected by vacuum filtration, rinsed with hexane, and dried in a vacuum-oven (76*C; ca. 10 mm of Hg) to afford 1.10 g (47%) of the desired material; mp 224-229.5*C; 1 H NMR (400 MHz, DMSO): 8 9.72 (s, 1H), 7.97 (dd, 1H, J=7.0, 8.7 Hz), 10 7.70 (d, 1H, J=1.5 Hz), 7.57 (dd, 1H, J=8.4, 1.9 Hz), 7.17 (d, 1H, J=8.2 Hz), 6.61-6.53 (m, 2H), 2.18 (s, 3H); 13 C NMR (100 MHz, DMSO): 8 169.87, 166.36 (d, JC-F= 2 4 9
.
4 Hz), 150.11 (d,
JC-F=
1 1
.
4 Hz), 139.83, 138.49, 136.07, 135.26 (d, JC-F= 1 1
.
5 Hz), 135.07, 125.60, 109.32, 104.98 (d, JC-F= 2 1
.
1 Hz), 99.54 (d, JC-F= 2 6
.
0 Hz), 89.43, 17.52; 15 19 F NMR (376 MHz, DMSO): 8 -104.00 to -104.07 (m); IR (KBr) 1670 (C=O stretch)cm- 1 ; MS (CI) M+1 = 372. Analysis calculated for C 14 Hi 1 FIN0 2 : C, 45.31; H, 2.99; N, 3.77. 20 Found: C, 45.21; H, 2.77; N, 3.64. (b) Preparation of 4-Fluoro-N-hydroxy-2-(4-iodo-2-methyl-phenylamino) benzamide To a stirred solution of 4-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid (0.6495 g, 0.001750 mol), 0-(tetrahydro-2H-pyran-2-yl)-hydroxylamine 25 (0.2590 g, 0.002211 mol), and diisopropylethylamine (0.40 mL, 0.0023 mol) in 31 mL of an equivolume tetrahydrofuran-dichloromethane solution was added 1.18 g (0.00227 mol) of solid PyBOP ([benzotriazolyloxy]tripyrrolidino phosphonium hexafluorophosphate, Advanced ChemTech) directly. The reaction mixture was stirred for 30 minutes after which time it was concentrated in vacuo. 30 The brown oil was treated with 10% aqueous hydrochloric acid. The suspension 60 WO 00/35435 PCT/US99/29591 was extracted with ether. The organic extraction was washed with 10% sodium hydroxide followed by another 10% hydrochloric acid wash, was dried (MgSO4) and concentrated in vacuo to afford 1.0 g of a light-brown foam. This intermediate was dissolved in 25 mL of ethanolic hydrogen chloride, and the solution was 5 allowed to stand at room temperature for 15 minutes. The reaction mixture was concentrated in vacuo to a brown oil that was purified by flash silica chromatography. Elution with a gradient (100 % dichloromethane to 0.6 % methanol in dichloromethane) afforded 0.2284 g of a light-brown viscous oil. Scratching with pentane-hexanes and drying under high vacuum afforded 10 0.1541 g (23%) of an off-white foam; mp 61-75*C; 1 H NMR (400 MHz, DMSO): 6 11.34 (s, 1H), 9.68 (s, 1H), 9.18 (s, 1H), 7.65 (d, 1H, J=1.5 Hz), 7.58 (dd, 1H, J=8.7, 6.8 Hz), 7.52 (dd, 1H, J=8.4, 1.9 Hz), 7.15 (d, 1H, J=8.4 Hz), 6.74 (dd, 1H, J=11.8, 2.4 Hz), 6.62 (ddd, 1H, J=8.4, 8.4, 2.7 Hz), 2.18 (s, 3H); 15 13 C NMR (100 MHz, DMSO): 8 165.91, 164.36 (d, JC-F= 2 4 7
.
1 Hz), 146.78, 139.18, 138.77, 135.43, 132.64, 130.60 (d, JC-F= 1 1
.
5 Hz), 122.23, 112.52, 104.72 (d, J=22.1 Hz), 100.45 (d, JC-F= 2 5
.
2 Hz), 86.77, 17.03; 19 F NMR (376 MHz, DMSO): 6 -107.20 to -107.27 (m); IR (KBr) 3307 (broad, O-H stretch), 1636 (C=O stretch) cm- 1 ; 20 MS (CI) M+1 = 387. Analysis calculated for CI 4 H1 2
FIN
2 02: C, 43.54; H, 3.13; N, 7.25. Found: C, 43.62; H, 3.24; N, 6.98. EXAMPLE 2a 25 5-Bromo-3,4-difluoro-N-hydroxy-2-(4-iodo-2-methyl-phenvlamino)-benzamide (a) Preparation of 5-Bromo-2,3,4-trifluorobenzoic acid To a stirred solution comprised of 1-bromo-2,3,4-trifluorobenzene (Aldrich, 99%; 5.30 g, 0.0249 mol) in 95 mL of anhydrous tetrahydrofuran cooled to -78*C was slowly added 12.5 mL of 2.0 M lithium diisopropylamide in 30 heptane/tetrahydrofuran/ethylbenzene solution (Aldrich). The mixture was stirred 61 WO 00/35435 PCT/US99/29591 for 1 hour and transferred by canula into 700 mL of a stirred saturated ethereal carbon dioxide solution cooled to -78*C. The cold bath was removed, and the reaction mixture was stirred for 18 hours at ambient temperature. Dilute (10%) aqueous hydrochloric acid (ca. 500 mL) was poured into the reaction mixture, and 5 the mixture was subsequently concentrated on a rotary evaporator to a crude solid. The solid product was partitioned between diethyl ether (150 mL) and aq. HCl (330 mL, pH 0). The aqueous phase was extracted with a second portion (100 mL) of diethyl ether, and the combined ethereal extracts were washed with 5% aqueous sodium hydroxide (200 mL) and water (100 mL, pH 12). These combined alkaline 10 aqueous extractions were acidified to pH 0 with concentrated aqueous hydrochloric acid. The resulting suspension was extracted with ether (2 x 200 mL). The combined organic extracts were dried (MgSO4), concentrated in vacuo, and subjected to high vacuum until constant mass was achieved to afford 5.60 g (88% yield) of an off-white powder; mp 139-142.5*C; 15 1 H NMR (400 MHz, DMSO): 8 13.97 (broad s, 1H, 8.00-7.96 (m, 1H); 1 3 C NMR (100 MHz, DMSO): 6 162.96, 129.34, 118.47, 104.54 (d,
JC-F=
2 2
.
9 Hz); 19 F NMR (376 MHz, DMSO): 8 -120.20 to -120.31 (m), -131.75 to -131.86 (m), -154.95 to -155.07 (m); 20 IR (KBr) 1696 (C=O stretch)cm- 1 ; MS (CI) M+1 = 255. Analysis calculated for C 74
H
2 1 BrF 3 02: C, 32.97; H, 0.79; N, 0.00; Br, 31.34; F, 22.35. Found: C, 33.18; H, 0.64; N, 0.01; Br, 30.14; F, 22.75. 25 (b) Preparation of 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino) benzoic acid To a stirred solution comprised of 1.88 g (0.00791 mol) of 2-amino 5-iodotoluene in 10 mL of tetrahydrofuran at -78*C was added 6 mL (0.012 mol) of a 2.0 M lithium diisopropylamide in tetrahydrofuran/heptane/ethylbenzene 30 (Aldrich) solution. The resulting green suspension was stirred vigorously for 62 WO 00/35435 PCT/US99/29591 10 minutes, after which time a solution of 1.00 g (0.00392 mol) of 5-bromo 2,3,4-trifluorobenzoic acid in 15 mL of tetrahydrofuran was added. The cold bath was subsequently removed, and the reaction mixture stirred for 18 hours. The mixture was concentrated, and the concentrate was treated with 100 mL of dilute 5 (10%) aqueous hydrochloric acid. The resulting suspension was extracted with ether (2 x 150 mL), and the combined organic extractions were dried (MgSO4) and concentrated in vacuo to give an orange solid. The solid was triturated with boiling dichloromethane, cooled to ambient temperature, and collected by filtration. The solid was rinsed with dichloromethane, and dried in the vacuum 10 oven (80*C) to afford 1.39 g (76%) of a yellow-green powder; mp 259.5-262*C; 1 H NMR (400 MHz, DMSO): 5 9.03 (s, 1H), 7.99 (dd, 1H, J=7.5, 1.9 Hz), 7.57 (dd, 1H, J=1.5 Hz), 7.42 (dd, 1H, J=8.4, 1.9 Hz), 6.70 (dd, 1H, J=8.4, 6.0 Hz), 2.24 (s, 3H); 19 F NMR (376 MHz, DMSO): S -123.40 to -123.47 (m); -139.00 to -139.14 (m); 15 IR (KBr) 1667 (C=O stretch)cm- 1 ; MS (CI) M+1 = 469. Analysis calculated for C 14
H
9 BrF 2
INO
2 : C, 35.93; H, 1.94; N, 2.99; Br, 17.07; F, 8.12; 1, 27.11. Found: C, 36.15; H, 1.91; N, 2.70; Br, 16.40; F, 8.46; 1, 26.05. 20 (c) Preparation of 5-Bromo-3,4-difluoro-N-hydroxy-2-(4-iodo-2-methyl phenylamino)-benzamide To a stirred solution comprised of 5-bromo-3,4-difluoro-2-(4-iodo 2-methyl-phenylamino)-benzoic acid (0.51 g, 0.0011 mol), 0-(tetrahydro-2H pyran-2-yl)-hydroxylamine (0.15 g, 0.0013 mol), and diisopropylethylamine 25 (0.25 mL, 0.0014 mol) in 20 mL of an equivolume tetrahydrofuran dichloromethane solution was added 0.6794 g (0.001306 mol) of solid PyBOP (Advanced ChemTech) directly. The reaction mixture was stirred at 24*C for 10 minutes, and then was concentrated to dryness in vacuo. The concentrate was suspended in 100 mL of 10% aqueous hydrochloric acid. The suspension was 30 extracted with 125 mL of diethyl ether. The ether layer was separated, washed with 75 mL of 10% aqueous sodium hydroxide, and then with 100 mL of dilute 63 WO 00/35435 PCT/US99/29591 acid. The ether solution was dried (MgSO4) and concentrated in vacuo to afford 0.62 g (100%) of an off-white foam. The foam was dissolved in ca. 15 mL of methanolic hydrogen chloride. After 5 minutes, the solution was concentrated in vacuo to an oil, and the oil was purified by flash silica chromatography. Elution 5 with dichloromethane: dichloromethane-methanol (99:1) afforded 0.2233 g (42%) of a yellow powder. The powder was dissolved in diethyl ether and washed with dilute hydrochloric acid. The organic phase was dried (MgSO4) and concentrated in vacuo to afford 0.200 g of a foam. This product was triturated with pentane to afford 0.1525 g of a powder that was repurified by flash silica chromatography. 10 Elution with dichloromethane afforded 0.0783 g (15%) of an analytically pure title compound, mp 80-90*C; 1H NMR (400 MHz, DMSO): 6 11.53 (s, 1H), 9.38 (s, 1H), 8.82 (s, 1H), 7.70 (dd, 1H, J=7.0, 1.9 Hz), 7.53 (s, 1H), 7.37 (dd, 1H, J=8.4, 1.9 Hz), 6.55 (dd, 1H, J=8.2, 6.5 Hz), 2.22 (s, 3H); 15 1 9 F NMR (376 MHz, DMSO): 8 -126.24 to -126.29 (m), -137.71 to -137.77 (m); IR (KBr) 3346 (broad, 0-H stretch), 1651 (C=O stretch)cm- 1 ; MS (CI) M+1 = 484. Analysis calculated for C1 4
H
1 0 BrF 2 1N 2 0 2 : C, 34.81; H, 2.09; N, 5.80. 20 Found: C, 34.53; H, 1.73; N, 5.52. Examples 3a to 12a in the table below were prepared by the general procedure of Examples 1 a and 2a. EXAMPLES 13a-77a Examples 13a to 77a were prepared utilizing combinatorial synthetic 25 methodology by reacting appropriately substituted phenylamino benzoic acids (e.g., as shown in Scheme 1) and hydroxylamines (e.g., (NHR6a )-O-R7a). A general method is given below: 30 To a 0.8-mL autosampler vial in a metal block was added 40 pL of a 0.5 M solution of the acid in DMF and 40 p.L of the hydroxylamine (2 M solution 64 WO 00/35435 PCT/US99/29591 in Hunig's base and 1 M in amine in DMF). A 0.5 M solution of PyBrOP was freshly prepared, and 50 pL were added to the autosampler vial. The reaction was allowed to stand for 24 hours. The reaction mixture was transferred to a 2-dram vial and diluted with 5 2 mL of ethyl acetate. The organic layer was washed with 3 mL of distilled water and the water layer washed again with 2 mL of ethyl acetate. The combined organic layers were allowed to evaporate to dryness in an open fume hood. The residue was taken up in 2 mL of 50% acetonitrile in water and injected on a semi-prep reversed phase column (10 mm x 25 cm, 5 pM spherical silica, 10 pore Size 115 A derivatized with C-18, the sample was eluted at 4.7 mL/min with a linear ramp to 100% acetonitrile over 8.5 minutes. Elution with 100% acetonitrile continued for 8 minutes.) Fractions were collected by monitoring at 214 nM. The desired fractions were evaporated using a Zymark Turbovap. The product was dissolved in chloroform and transferred to a preweighed vial, 15 evaporated, and weighed again to determine the yield. The structure was confirmed by mass spectroscopy. 65 WO 00/35435 PCT/US99/29591 EXAMPLES 3a-77a Example Compound Melting MS No. Point (*C) (M-H+) 3a 2-(4-bromo-2-methyl-phenylamino)-4-fluoro-N- 56-75 dec 523 hydroxy-benzamide 4a 5-Chloro-N-hydroxy-2-(4-iodo-2-methyl- 65 dec phenylamino)-benzamide 5a 5-Chloro-N-hydroxy-2-(4-iodo-2-methyl- 62-67 phenylamino)-N-methyl-benzamide 6a 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N- 105-108 (terahydropyran-2-yloxy)benzamide 7a 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N- 64-68 methoxybenzamide 8a 4-Fluoro-N-hydroxy-2-(4-fluoro-2-methyl- 119-135 phenylamino)-benzamide 9a 4-Fluoro-N-hydroxy-2-(2-methyl phenylamino)- 101-103 benzamide 10a 4-Fluoro-2-(4-fluor-2-methyl-phenylamino)-N- 142-146 (terahydropyran-2-yloxy)benzamide 11a 4-Fluoro-N-hydroxy-2-(4-cluoro-2-methyl- 133.5-135 phenylamino)-benzamide 66 WO 00/35435 PCT/US99/29591 Example Compound Melting MS No. Point (*C) (M-H+) 12a 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N- 107-109.5 phenylmethoxy-benzamide 13a 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N- 399 methoxy-benzamide 14a 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)- 417 N-methoxy-benzamide 15a 2-(4-Bromo-2-methyl-phenylamino)- 369 3,4-difluoro-N-methoxy-benzamide 16a 2-(4-Bromo-2-methyl-phenylamino)-N-ethoxy- 342* 3,4-difluoro-benzamide (M-EtO) 17a 5-Bromo-N-ethoxy-3,4-difluoro-2-(4-iodo- 509 2-methyl-phenylamino)-benzamide 18a 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)- 445 N-isopropoxy-benzamide 19a 2-(4-Bromo-2-methyl-phenylamino)- 397 3,4-difluoro-N-isopropoxy-benzamide 20a 4-Fluoro-N-(furan-3-ylmethoxy)-2-(4-iodo- 465 2-methyl-phenylamino)-benzamide 67 WO 00/35435 PCTIUS99/29591 Example Compound Melting MS No. Point (*C) (M-H+) 21a 3,4-Difluoro-N-(furan-3-ylmethoxy)-2-(4-iodo- 483 2-methyl-phenylamino)-benzamide 22a 2-(4-Bromo-2-methyl-phenylamino)- 435 3,4-difluoro-N-(furan-3-ylmethoxy)-benzamide 23a 5-Bromo-3,4-difluoro-N-(furan-3-ylmethoxy)- 561 2-(4-iodo-2-methyl-phenylamino)-benzamide 24a 5-Bromo-N-(but-2-enyloxy)-3,4-difluoro- 536 2-(4-iodo-2-methyl-phenylamino)-benzamide 25a 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N- 423 (prop-2-ynyloxy)-benzamide 26a 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)- 441 N-(prop-2-ynyloxy)-benzamide 27a 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)- 455 N-(1 -methyl-prop-2-ynyloxy)-benzamide 28a 2-(4-Bromo-2-methyl-phenylamino)- 407 3,4-difluoro-N-(1 -methyl-prop-2-ynyloxy) benzamide 29a N-(But-3-ynyloxy)-3,4-difluoro-2-(4-iodo- 455 2-methyl-phenylamino)-benzamide 68 WO 00/35435 PCTIUS99/29591 Example Compound Melting MS No. Point (*C) (M-H+) 30a 2-(4-Bromo-2-methyl-phenylamino)-N-(but- 407 3-ynyloxy)-3,4-difluoro-benzamide 31a 5-Bromo-N-(but-3-ynyloxy)-3,4-difluoro- 533 2-(4-iodo-2-methyl-phenylamino)-benzamide 32a 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)- 517 N-(3-phenyl-prop-2-ynyloxy)-benzamide 33a 3,4-Difluoro-2-(4-bromo-2-methyl- 469 phenylamino)-N-(3-phenyl-prop-2-ynyloxy) benzamide 34a 3,4-Difluoro-N-[3-(3-fluoro-phenyl)-prop- 535 2-ynyloxy]-2-(4-iodo-2-methyl-phenylamino) benzamide 35a 2-(4-Bromo-2-methyl-phenylamino)- 487 3,4-difluoro-N-[3-(3-fluoro-phenyl)-prop 2-ynyloxy]-benzamide 36a 3,4-Difluoro-N-[3-(2-fluoro-phenyl)-prop- 535 2-ynyloxy]-2-(4-iodo-2-methyl-phenylamino) benzamide 37a 5-Bromo-3,4-difluoro-N-[3-(2-fluoro-phenyl)- 613 prop-2-ynyloxy]-2-(4-iodo-2-methyl phenylamino)-benzamide 69 WO 00/35435 PCTIUS99/29591 Example Compound Melting MS No. Point (*C) (M-H+) 38a 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)- 557* N-(3-methyl-5-phenyl-pent-2-en-4-ynyloxy)- *(M+H) benzamide 39a 2-(4-Bromo-2-methyl-phenylamino)- 510 3,4-difluoro-N-(3-methyl-5-phenyl-pent-2-en 4-ynyloxy)-benzamide 40a N-Ethoxy-3,4-difluoro-2-(4-iodo-2-methyl- 431 phenylamino)-benzamide 41a 2-(4-Bromo-2-methyl-phenylamino)-N-ethoxy- 383 3,4-difluoro-benzamide 42a 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N- 427 propoxy-benzamide 43a 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)- 445 N-propoxy-benzamide 44a 2-(4-Bromo-2-methyl-phenylamino)- 397 3,4-difluoro-N-propoxy-benzamide 45a 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl- 523 phenylamino)-N-propoxy-benzamide 46a 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N- 427 isopropoxy-benzamide 70 WO 00/35435 PCT/US99/29591 Example Compound Melting MS No. Point (*C) (M-H+) 47a 3,4-Difluoro-2-(4-iodo-2-methyl-phenyl amino)- 445 N-isopropoxy-benzamide 48a 2-(4-Bromo-2-methyl-phenylamino)- 397 3,4-difluoro-N-isopropoxy-benzamide 49a 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl- 523 phenylamino)-N-isopropoxy-benzamide 50a N-Cyclobutyloxy-3,4-difluoro-2-(4-iodo- 457 2-methyl-phenylamino)-benzamide 51a 2-(4-Bromo-2-methyl-phenylamino)-N- 409 cyclobutyloxy-3,4-difluoro-benzamide 52a N-Cyclopentyloxy-4-fluoro-2-(4-iodo-2-methyl- 453 phenylamino)-benzamide 53a N-Cyclopentyloxy-3,4-difluoro-2-(4-iodo- 471 2-methyl-phenylamino)-benzamide 54a 2-(4-Bromo-2-methyl-phenylamino)-N- 423 cyclopentyloxy-3,4-difluoro-benzamide 55a N-Cyclopropylmethoxy-4-fluoro-2-(4-iodo- 439 2-methyl-phenylamino)-benzamide 56a N-Cyclopropylmethoxy-3,4-difluoro-2-(4-iodo- 457 2-methyl-phenylamino)-benzamide 71 WO 00/35435 PCTIUS99/29591 Example Compound Melting MS No. Point (*C) (M-H+) 57a 2-(4-Bromo-2-methyl-phenylamino)-N- 409 cyclopropylmethoxy-3,4-difluoro-benzamide 58a 5-Bromo-N-cyclopropylmethoxy-3,4-difluoro- 435 2-(4-iodo-2-methyl-phenylamino) 59a 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N- 505 (2-phenoxy-ethoxy)-benzamide 60a 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)- 523 N-(2-phenoxy-ethoxy)-benzamide 61a 2-(4-Bromo-2-methyl-phenylamino)- 475 3,4-difluoro-N-(2-phenoxy-ethoxy)-benzamide 62a 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N- 481 (thiophen-2-ylmethoxy)-benzamide 63a 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)- 499 N-(thiophen-2-ylmethoxy)-benzamide 64a 2-(4-Bromo-2-methyl-phenylamino)- 451 3,4-difluoro-N-(thiophen-2-ylmethoxy) benzamide 65a 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N- 439 (2-methyl-allyloxy)-benzamide 72 WO 00/35435 PCT/US99/29591 Example Compound Melting MS No. Point (*C) (M-H+) 66a 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)- 457 N-(2-methyl-allyloxy)-benzamide 67a 2-(4-Bromo-2-methyl-phenylamino)- 410 3,4-difluoro-N-(2-methyl-allyloxy)-benzamide 68a N-(But-2-enyloxy)-4-fluoro-2-(4-iodo-2-methyl- 439 phenylamino)-benzamide 69a N-(But-2-enyloxy)-3,4-difluoro-2-(4-iodo- 457 2-methyl-phenylamino)-benzamide 70a 2-(4-Bromo-2-methyl-phenylamino)-N-(but- 410 2-enyloxy)-3,4-difluoro-benzamide 71a 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)- 441 N-(prop-2-ynyloxy)-benzamide 72a N-(But-3-ynyloxy)-3,4-difluoro-2-(4-iodo- 455 2-methyl-phenylamino)-benzamide 73a 2-(4-Bromo-2-methyl-phenylamino)-N- 449 (4,4-dimethyl-pent-2-ynyloxy)-3,4-difluoro benzamide 74a N-(But-2-enyloxy)-3,4-difluoro-2-(4-iodo- 457 2-methyl-phenylamino)-benzamide 73 WO 00/35435 PCT/US99/29591 Example Compound Melting MS No. Point (*C) (M-H+) 75a 2-(4-Bromo-2-methyl-phenylamino)-N-(but- 410 2-enyloxy)-3,4-difluoro-benzamide 76a N-(3-tert-butyl-propyn-2-yl)oxy-4-fluoro- 479 2-(4-iodo-2-methyl-phenylamino)-benzamide 77a 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N- 577 phenylmethoxy-benzamide PHYSICAL DATA FOR SELECTED COMPOUNDS PD 0171984 5 mp 80-90 C PD 0184161 mp 174-175 *C PD 0203311 mp 141-144 C 10 PD 0297189 mp 167-169*C 'H-NMR (400 MHz; DMSO) 6 11.70 (s, 1H), 8.59 (s, 1H), 7.55 (s, 1H), 7.43 (d, 1H, J=6.5 Hz), 7.27 (d,1H, J=8.7 Hz), 6.46 (m, 111), 3.42 (d, 2H, J=7.0 Hz), 0.84 15 (m, 1H), 0.27 (m, 2H), 0.00 (m, 2H) 74 WO 00/35435 PCT/US99/29591 PD 0297190 mp 125.5-133 "C 5 'H-NMR (400 MHz; DMSO) 8 11.48 (s, 1H), 8.32 (s, 1H), 7.34 (d, 1H, J=7.5 Hz), 7.28 (d, 2H, J=8.2 Hz), 6.48 (d, 2H, J=7.7 Hz), 3.32 (d, 2H, J=6.8 Hz), 0.81 (m, 1H), 0.28 (m, 2H), 0.00 (m, 2H) PD 0296771 mp 266.7-268.9 "C 10 'H-NMR (400 MHz; DMSO) 8 13.85 (broad s, 1H), 8.99 (s, 1H), 7.87 (dd, 1H, J=7.9, 2.1 Hz), 7.55 (d,2H, J=8.6 Hz), 6.82 (dd, 2H, J=8.7, 2.8 Hz) PD 0296770 mp 293.2-296.3 "C 15 'H-NMR (400 MHz; DMSO) 8 14.05 (broad s, 1H), 9.21 (s, 1H), 7.93 (dd, 1H, J=7.8, 2.2 Hz), 7.82 (d,1H, J=1.9 Hz), 7.54 (dd, 1H, J=8.6, 1.9 Hz), 6.82 (dd, 1H, J=8.6, 6.7 Hz) PD 0296767 20 mp 249-251 *C 1 H-NMR (400 MHz; DMSO) 6 13.99 (broad s, 1H), 9.01 (s, 1H), 7.90 (dd, 1H, J=7.9, 2.3 Hz), 7.58 (d,1H, J=1.6 Hz), 7.42 (dd, 1H, J=8.4, 1.9 Hz), 6.69 (dd, 1H, J=8.4, 6.0 Hz), 2.24 (s, 3H) 25 PD 298127 mp 127-135 *C 5-chloro-N-cyclopropyl methoxy-3,4-difluoro-2-[4-iodo-2-methyl phenylamino]benzamide 30 'H NMR (440 MHz; DMSO) 8 11.64 (s, 1H), 8.28 (s, 1H), 7.38 (dd, 1H, J=7.6, 1.7 Hz), 7.31 (d, 1 H, J=1.2 Hz), 7.15 (dd, 1H, J=8.5, 1.7 Hz), 3.35 (d, 2H, J=7.3 Hz), 2.01 (s, 3H), 0.83 (m, 1H), 0.28 (m,2H), 0.01 (m, 2H) 35 75 WO 00/35435 PCT/US99/29591 BIOLOGICAL ASSAYS Example 1 Inhibition of IL-2 Production Induced by Concanavalin A (Con A) 5 Several of the phenyl amine MEK inhibitors described above have been evaluated in a number of assays which establish their utility in preventing the rejection of transplants in mammals. One such assay measured the ability of a test compound to inhibit the production of IL-2 from T cells (T lymphocytes) present in human peripheral blood mononuclear cells (HPBMC). In this assay, the cells 10 (HPBMC) were prepared by first centrifuging tubes of heparinized blood (obtained from normal healthy volunteers) at 1400 rpm for 10 minutes at room temperature. The interphase containing mostly leukocytes was removed and added to a 50 mL centrifuge tube, and diluted with phosphate buffered saline (PBS) to a volume of 40 mL. The diluted PBS solution was added to a 50 mL centrifuge tube 15 containing 7 mL of Histopague (Sigma, Sp. Gr. 1.077). The mixture was centrifuged at 2200 rpm for 20 minutes at room temperature. The middle layer, comprised mostly of peripheral blood mononuclear cells (PBMC), was removed and added to a clean 50 mL centrifuge tube. These cells were diluted with PBS to a volume of 30 mL, and centrifuged at 1000 rpm for 10 minutes at room 20 temperature. The supernatant was removed, and the remaining cells were washed twice with 30 mL portions of PBS. The PBMC were resuspended in medium (Roswell Park Memorial Institute No. 1640 (RPMI-1640), from Gibco BRL, Gaithersburg, MD), and 10% fetal bovine serum (FBS) culture medium. The cells were adjusted to 2.5 x 106 cells/mL. 25 The compounds to be tested were prepared by dissolving them in dimethylsulfoxide (DMSO) to a concentration of 30 micromolar. Additional dilutions were made in RPMI-1640, and then in RPMI-1640 containing 1% DMSO so that the final in-well concentration of DMSO was 0.25% in all wells. Concanavalin A (Con A) was purchased from CalBiochem (Catalog No. 30 234567). A stock solution was prepared by dissolving 250 mg of Con A in 10 mL of sterile water (25 mg/mL). 76 WO 00/35435 PCT/US99/29591 The assay was carried out by adding 50 piL of the diluted test compounds to appropriate wells of a plate. To the wells were added 100 p.L of the PBMC cell solution (2.5 x 106 cells/mL). The mixtures were pre-incubated for 15 minutes at 37*C, in a 5% carbon dioxide incubator. For the HPBMC assay, 50 [L of the 5 Con A solution (80 ptg/mL Con A in RPMI-1640) were added to the appropriate wells. For the HWB assay, 50 ptL of a Con A solution (800 pg/mL Con A in RPMI-1640) were added to the appropriate wells. Control wells contained medium plus 50 tL of RPMI-1640. The well plates were incubated for 2 days at 37*C in a 5% carbon dioxide incubator. At the end of Day 2, the plates were 10 centrifuged at 2200 rpm for 5 minutes at 0-4*C. Samples of supernatant (150 pL) were removed from each well and stored at -20*C until analyzed. Each sample was analyzed by an IL-2 ELISA kit (No. D2050 from R & D Systems, Minneapolis, MN) to measure the content of IL-2. The results of the foregoing assay are shown in Figures 1 and 9. A 15 preferred compound to be used in accordance with this invention is 2-(2-chloro 4-iodo-phenylamino)-N-cyclopropylmethoxy-3,4-difluorobenzamide, also known as PD 184352. Figure 1 shows that no IL-2 is produced by unstimulated cells, but large amounts are produced in the presence of Con A. The Figure shows that PD 184352 causes a dramatic dose dependent inhibition of IL-2 production, and 20 has an IC 5 0 of 71 nM. Figure 9 shows the inhibition of IL-2 production in cells caused by several of the phenyl amine MEK inhibitors of Formulas I and II, compared to known immunosuppressive agents dexamethasone, a steroid, which is 9-fluoro 11p , 17,21 -trihydroxy- 1 6a-methylpregna- 1,4-diene-3,20-dione, and rolipram, a 25 phosphodiesterase-4 inhibitor which is 4-[3-(cyclopentyloxy)-4-methoxyphenyl] 2-pyrrolidinone. The data establish that the phenyl amine MEK inhibitors are in general very potent in their ability to inhibit IL-2 production. Example 2 Inhibition of IL-2 Production Induced by Anti-CD3 & Anti-CD28 77 WO 00/35435 PCT/US99/29591 Stimulation of T cells through direct activation of the T cell receptor is felt to be more representative of physiologic T cell activation than when cells are activated by mitogens, such as Con A. The T cell receptor is a complex, multi protein receptor comprised in part of a set up proteins collectively called CD3. In 5 order for T cells to produce IL-2, they must also be activated by a co-receptor. The most prominent and best-characterized T cell co-receptor is CD28. Monoclonal antibodies to CD3 and CD28 and be used together to induce release of IL-2. Anti-CD3 was purchased from BioSource Int. (catalog #AHS2812). A 10 working solution was prepared containing 10 pg/mL of anti-CD3 in PBS. A 100 ptL aliquot was added to appropriate wells and incubated for 3 hours at 37"C, and then unbound anti-CD3 was washed off with PBS. Anti-CD28 was purchased from BioSource Int. (catalog #AH0312) and was added as a solution (0.5 pg/mL) to appropriate wells after addition of HPBMC and MEK inhibitor. 15 HPBMC were prepared as described in Example 1 and stimulated with concentrations of anti-CD3 and anti-CD28 determined from pilot studies to provide a high degree of T cell activation, and hence IL-2 release. After a 2-day culture period in a humidified 37 0 C incubator containing 5% Co 2 in air, supernatant was collected and assayed for IL-2 as described in Example 1. 20 The results of the foregoing assay are shown in Figure 2. A preferred compound to be used in accordance with this invention is PD 184352. The Figure shows that no IL-2 is produced by unstimulated cells, but large amounts are produced in the presence of anti-3 plus anti-CD28. The Figure shows that PD 184352 causes a dramatic dose dependent inhibition of IL-2 production, and 25 has an IC 5 0 of 47 nM. 78 WO 00/35435 PCT/US99/29591 Example 3 Inhibition of Interferon-y Production The foregoing procedure was followed to evaluate the ability of the phenyl amine MEK inhibitors of Formulas I and II to inhibit the release of interferon 5 gamma (IFN-gamma) from human peripheral blood mononuclear cells (HPBMC) and human whole blood (HWB). The cell samples and compound samples were prepared by the general procedure described above. The assays of the incubated well plates were carried out using an IFN-gamma ELISA kit (No. DIFOO from R & D Systems). The results of the assays are shown in Figures 3 and 10. Figure 3 10 shows that Con A causes a large production of IFN-gamma, and that such production is totally inhibited by PD 184352 at some concentrations. The Figure shows that the IC 5 0 for PD 184352 against IFN-gamma is 148 nM. Figure 10 shows the dose dependent inhibition of IFN-gamma caused by various phenyl amine MEK inhibitors of Formulas I and II, and the activity of 15 known immunosuppressive agents rolipram and dexamethasone. The data establish that the phenyl amine MEK inhibitors are much more potent that rolipram, and cause almost 100% inhibition at concentrations of 1 IM or higher. The ability of the MEK inhibitors of Formulas I and II to inhibit IFN-gamma production establishes that they can be used for the prophylaxis of transplants of 20 organs, limbs, cells, and tissues in mammals. Example 4 Human Mixed Lymphocyte Reaction Several of the MEK inhibitors which are to be used in the method of this invention have been evaluated in an in vitro test in which lymphocytes (or 25 leukocytes) from one donor (eg, the potential recipient of a transplant) are cultured in the presence of leukocytes from another donor (eg, the potential transplant donor, generally a living related donor, not cadaveric donors). This test measures the degree of histoincompatibility. The assay is a mixed lymphocyte (or leukocyte) reaction, and is referred to as the "MLR". In this assay, inhibition of 30 tritiated thymidine ( 3 H-TDR) incorporation is measured. Tritiated thymidine was supplied from Amersham (Catalog No. TRK.758, 250 ptCi). The commercial 79 WO 00/35435 PCT/US99/29591 product was diluted in RPMI- 1640 in a 50 mL conical centrifuge tube to provide a working stock solution of 5-10 iCi/mL. Cells and test compounds were prepared as described above. The compounds and cells were incubated at 37*C in a 5% carbon dioxide incubator. On Day 6, each well of the assay plate was labeled with 5 the 3 H-TDR working stock solution (total of 0.1 - 0.5 ptCi per well). The plates were incubated an additional 6 hours following labeling. The plate samples were harvested using a multichannel harvester, and the radioactivity of each sample was counted using a betaplate Wallace 1205 counter. Figure 4 shows the activity of PD 184352 in the human MLR assay. The 10 activity is measured as counts per minute (CPM) of tritiated thymidine ( 3 H-TDR) uptake. The Figure shows that untreated MLR values are in excess of 4500 CPM, whereas the test compound causes a dose dependent inhibition of 3 H-TDR uptake, with almost total inhibition occurring at 10 pM. The IC 5 0 for PD 184352 was established as 186 nM. 15 Figure 11 shows the activity of several phenyl amine MEK inhibitors in the MLR assay, compared to dexamethasone. The data presented in Figures 4 and 11 further establish that the selective MEK inhibitors of Formulas I and II are useful for preventing the rejection of transplanted organs, tissues, cells, and limbs in mammals. 20 Example 5 Inhibition of T-Cell Proliferation induced by Con A Another measure of immunosuppressive activity is a compound's ability to block the growth of T cells. Uncontrolled proliferation of T cells leads to rejection of transplanted organs, tissues, cells, and limbs in mammals. Immunological 25 studies have established that cyclosporine A blocks activation of T cells, and that this is partly the result of inhibition of the synthesis of interleukin-2, the main growth factor for T cells. The assay was carried out by following the general procedure described above for preparing cells and test compounds, and 3 H-TDR inhibition was measured. Con A was used to induce T-cell proliferation. 30 Figure 5 shows the degree to which PD 184352 inhibits T-cell proliferation. Namely, the compound causes about 50% inhibition of the Con A 80 WO 00/35435 PCT/US99/29591 induced proliferation at the lowest dose tested (0.12 tM), and causes almost total inhibition at the highest dose tested (10.0 pM). The IC 5 0 for the compound was determined to be 340 nM. Figure 12 shows that all of the phenyl amine MEK inhibitors that were 5 tested caused a dramatic and dose dependent inhibition of T-cell proliferation. Example 6 Inhibition of T-Cell Proliferation induced by Phytohemagglutinin (PHA) The T-cell inhibition study was carried out using the agent PHA to induce the proliferation. Figure 6 shows the effects of PD 184352. In this study, the test 10 compound failed to cause inhibition at the low dose (0.12 pLM), but caused a measurable inhibition at all other doses, with almost total inhibition at the high dose (10 pM). The IC 5 0 was determined to be 1.9 pM in this assay. The data further establish the ability of the phenyl amine MEK inhibitor to inhibit T-cell proliferation, and thereby to be useful in the prophylaxis of transplant rejections in 15 mammals. Example 7 Toxicity Assay As noted above, the MEK inhibitors to be used in the method of this invention are potent inhibitors of transplant rejection, while at the same time have 20 little or no toxicity, a feature which severely limits the clinical usefulness of commercial immunosuppressive agents. The toxic effects of the compounds were evaluated in an assay using MTT, which is a chemical substance known as 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide. MTT changes color when it is activated by a cell, and that color change can be measured by 25 routine methods. Only living cells can change the color of MTT. For this assay, living U-937 cells were obtained from American Type Culture Collection (Rockville, MD). PD 184352 was added to the cells in plate wells, and the cells were incubated as described above. Following the incubation period, the color change of MTT was measured using a spectrophotometer. Figure 7 shows that 30 PD 184352 caused no toxicity at concentrations below 33 ptM, and caused only 81 WO 00/35435 PCT/US99/29591 slight color change even at concentrations as high as 100 ptM. The dose of PD 184352 required to cause cell death of one-half of the cells (the TC 5 0 ) was thus determined to be greater than 100 piM. These data establish that the phenyl amine MEK inhibitors are essentially devoid of any toxic effects in this assay. 5 Figure 13 shows the toxicity of several of the phenyl amine MEK inhibitors when evaluated in the MTT assay. The data establish that all of the compounds evaluated have a very favorable therapeutic index, ie, biological efficacy for prophylaxis of transplant rejection vs toxicity. Thus, the compounds will find widespread use in the clinical setting for preventing and controlling 10 transplant rejection in mammals. Figure 8 shows the relative activities of several of the phenyl amine MEK inhibitors of Formulas I and II, compared with the activities of rolipram and dexamethasone, in a number of the assays described above. The Figure establishes that the phenyl amine MEK inhibitors are, in general, as active as or more active 15 than the known agents when evaluated in standard assays which establish utility of compounds in the prophylaxis of transplant rejections in mammals. Much of the foregoing data is summarized below in Pharmacological Table 1. The Table presents the in vitro effects of several compounds to be used in the method of this invention, together with several comparator 20 immunosuppressive agents, on human leukocytes. The data are concentrations of test compounds required to cause a 50 percent inhibition of the measured parameter (the IC 50 ), except in the case of the toxicity data, which is presented as
TC
5 0 (concentration required to produce toxicity in 50 percent of the cells). In the Table, "APK" refers to activity of compounds in a cascade assay, wherein a 25 compound inhibits a MEK enzyme, thereby preventing phosphorylation of another enzyme, namely a MAP (mitogen activated protein) kinase, which otherwise would cause phosphorylation of a substrate, in this assay said substrate being myelin basic protein The comparator agent U0126 (in Pharmacological Table 1) is 1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio]butadiene, an 30 immunosuppressive compound described in US Patent No. 2,779,780. 82 WO 00/35435 PCT/US99/29591 PHARMACOLOGICAL TABLE I MEK Inhibitors: In Vitro Effects on Human Leukocytes (All data are mean (*) IC 5 0 s or % inhibition at the concentration given, except toxicity data (MTT) which are TC 5 0 S) APK Human IFN- U937 MTT 'H-TDR 'H-TDR 'H-TDR
IC
5 0 IL-2 gamma TC 5 0 PHA MLR Con A (nM) (IM) (pM) (pM) or (M) (pM) (pM) (% dead) 171984-0000 3.0 0.019 *0.034 50.6 *2.4 0.35 *0.19 177098-0000 14.0 0.006 *0.076 *29.9 *NJ *NJ *3.9 177168-0000 18.0 0.052 *0.17 *13.9 *2.5 0.69 *0.52 180841-0000 4.4 *0.11 *0.21 *5.8 *ND *NJ *ND 184161-0000 1.6 *0.19 *0.15 *12.6 *1.8 0.53 *0.61 184352-0000 1.3 0.068 *0.14 >100 (6%) *4.5 0.64 *0.52 184386-0000 1.4 0.039 *0.040 61 *4.0 0.41 *0.31 185625-0000 5.1 0.071 *0.12 *12.4 *NJ *0.39 *4.0 185848-0000 1.0 0.018 *0.024 38.1 *NJ *0.51 *NJ 188563-0000 1.3 0.013 *0.15 40 *NJ *0.17 *NJ 198306-0000 8.0 0.037 *0.15 13.1 *1.40 *1.8 *1.9 203311-0000 -- 0.032 *0.10 *32.2 *ND *0.076 *ND ND = not determined. NJ no judgment: Studies indeterminant or incomplete. PHARMACOLOGICAL TABLE 1 (cont) MEK Inhibitors: In Vitro Effects on Human Leukocytes (All data are mean (*) IC 5 0 s or % inhibition at the concentration given, except toxicity data (MTT) which are TC 5 0 s) APK Human IFN- U937 MTT -H-TDR 3 H- 3 H-TDR
IC
5 0 IL-2 gamma TC 5 0 PHA TDR Con A (nM) (pM) (pM) (pM) or (pM) MLR (pM) (% dead) (M) STANDARDS 98059-0000 >1000 *7.4 *5.8 >100 (0%) *>10 *5.1 *>10 U0126 -- 0.077 *0.25 >100 (0%) *NJ *0.83 *NJ (PD 199601) Rolipram -- 0.094 *0.65 *ND *>10 *3.5 *NJ Dexamethasone - *0.005 *0.005 >100 (0%) *>10 *0.01 *<0.041 The foregoing extensive biological evaluations clearly establish the selective MEK inhibitors described above, especially the phenyl amines of 5 Formulas I and II, are well-suited to the prophylaxis of transplant rejections in mammals, preferably humans. Like other immunosuppressive agents, the MEK inhibitors can be used in combination with other such agents for even better 83 WO 00/35435 PCT/US99/29591 results. For example, the MEK inhibitors can be combined clinically with agents such as cyclosporine A and FK 506, another well-known immunosuppressive agent. The agents can be combined into the same formulation, but are more typically administered in their individual formulated doses, and normally at the 5 dose levels routinely used for the individual agents when used alone; however, lower or higher doses can be used if desired. The individual agents can be packaged together for convenience of the medical practitioner, for example in a kit or the like. Example 8 10 The selective MEK inhibitors to be used in the method of this invention will additionally be evaluated in in vivo assays that establish their ability to prevent and control transplant rejections. A typical in vivo assay is an allogeneic mouse ear-heart model using neonatal or newborn mouse hearts. Mice of the BL/6 to C3H strain will be used as test animals. Ten mice will be treated with a 15 MEK inhibitor. Three vehicle control allografts will be included, as well as three isografts, as control animals. Mice will be dosed at 50 mg/kg twice each day, until grafts are rejected, or until there is evidence of a definite anti-rejection effect. The MEK inhibitor being evaluated will be dissolved in a dosing solution which is 10% ethanol, 10% Cremophor EL (Sigma, Cat. No. C-5135), and 80% water 20 (v/v/v). The test animals are dosed orally using a tuberculin syringe and a mouse oral gavage tube. The dosing ratio is 0.1 mL of solution per each 20 g of mouse weight. The MEK inhibitor (300 mg) to be tested is placed in a 50 mL conical tube, and 3.0 mL of ethanol is added. The tube is capped to retard evaporation and vortexed to facilitate dissolution. The Cremophor EL (3.0 mL) is added, followed 25 by the addition of 24.0 mL of water. The 30 mL dosing solution is vortexed, and stored at 5C until used. If any grafts are rejected at any time during the study, the animal is sacrificed by dry ice (C0 2 ) asphyxiation as soon as graft rejection is determined. All specimens are obtained immediately after sacrificing the animals, and placed 30 in 10-20 mL of buffered formalin. If all allografts survive to the end of the study, one-half are placed in the buffered formalin, and the other half are frozen for subsequent analysis. The following tissues are collected for histopathology and 84 WO 00/35435 PCT/US99/29591 phospho-ERK analysis: ear bearing the allograft (or isograft); ipsilateral cervical lymph nodes; contralateral cervical lymph nodes; the spleen; and heparinized blood collected by cardiac puncture for determination of drug concentration. If transplants are still surviving on Day 50, the study is terminated, and the above 5 noted specimens are collected and analyzed. The method of this invention provides for both prophylaxis and maintenance of patients who have undergone a transplant or are scheduled to undergo a transplant. Evaluation of one MEK inhibitor, 2-(2-Methyl 4-iodophenylamino)-N-cyclopropylmethoxy-3,4,5-trifluorobenzamide 10 (PD 198306)) was performed using the aforementioned protocol, but no enhancement of graft survival was observed (data not shown). This may be the result of any or a combination of several factors, among which is insufficient exposure of target cells to an adequate and sustained concentration of the MEK inhibitor. Survival time of isografts in mice treated with PD 198306 was 15 somewhat shortened, which may suggest that MEK inhibitors might be more efficacious for graft maintenance. D. Other Embodiments From the above disclosure and examples, and from the claims below, the 20 essential features of the invention are readily apparent. The scope of the invention also encompasses various modifications and adaptations within the knowledge of a person of ordinary skill. Examples include a disclosed compound modified by addition or removal of a protecting group, or an ester, pharmaceutical salt, hydrate, acid, or amide of a disclosed compound. Publications cited herein are 25 hereby incorporated by reference in their entirety. What is claimed is: 85

Claims (15)

1. A method for preventing and controlling the rejection, in a patient, of a transplanted organ, cell, tissue, or limb, said method comprising administering to the patient who has undergone a transplant, or who is 5 scheduled to undergo a transplant, an effective immunosuppressive amount of a MEK inhibitor.
2. A method according to Claim 1 wherein the MEK inhibitor administered is 2-(2-amino-3-methoxyphenyl)-4-oxo-4H-[1]benzopyran. 10
3. The method according to Claim 1, wherein said MEK inhibitor is a selective MEK 1 or MEK 2 inhibitor.
4. The method according to Claim 1 wherein the MEK inhibitor is a compound of Formula I 2Z N 15 R5 Br or I R3 R4 wherein: RI is hydrogen, hydroxy, C 1 -C 8 alkyl, C 1 -C 8 alkoxy, halo, trifluoromethyl, or CN; R 2 is hydrogen; 20 R 3 , R 4 , and R 5 independently are hydrogen, hydroxy, halo, trifluoromethyl, C 1 -C 8 alkyl, C 1 -C 8 alkoxy, nitro, CN, or -(0 or NH)m-(CH2)n-R9, where R 9 is hydrogen, hydroxy, COOH, orNRIORII; n is 0-4; 86 WO 00/35435 PCT/US99/29591 m is 0 or 1; R 10 and R 11 independently are hydrogen or CI-C 8 alkyl, or taken together with the nitrogen to which they are attached can complete a 3-10 member cyclic ring optionally containing 1, 2, or 5 3 additional heteroatoms selected from 0, S, NH, or N-C 1 -Cg alkyl; Z is COOR 7 , tetrazolyl, CONR 6 R 7 , CONHNR 10 RI 1 , or CH 2 OR 7 ; R 6 and R 7 independently are hydrogen, CI-C 8 alkyl, C 2 -C 8 alkenyl, C 2 -C 8 alkynyl, (CO)-Cl-C 8 alkyl, aryl, heteroaryl, C 3 -C 10 cycloalkyl, or 10 C 3 -C 10 (cycloalkyl optionally containing one, two, or three heteroatoms selected from 0, S, NH, or N alkyl); or R 6 and R 7 together with the nitrogen to which they are attached complete a 3-10 member cyclic ring optionally containing 1, 2, or 3 additional heteroatoms selected from 0, S, NH, or N alkyl; and wherein any of the foregoing alkyl, alkenyl, aryl, 15 heterocyclic, and alkynyl groups can be unsubstituted or substituted by halo, hydroxy, C 1 -C 6 alkoxy, amino, nitro, CI-C 4 alkylamino, di(CI C 4 )alkylamino, C 3 -C 6 cycloalkyl, phenyl,phenoxy, C 3 -C 5 heteroaryl, or C 3 C 5 heteroaryloxy; or a pharmaceutically acceptable salt, ester, amide, or prodrug thereof 20 5. The method according to Claim 4 wherein the MEK inhibitor is a compound selected from: [4-Chloro-2-(1H-tetrazol-5-yl)-phenyl-(4-iodo-2-methyl-phenyl) amine; (4-iodo-2-methyl-phenyl)-[2-(1H-tetrazol-5-yl)-phenyl]amine; 25 [4-nitro-2-(1H-tetrazol-5-yl)-phenyl-(4-iodo-2-methyl-phenyl) amine; 4-Fluoro-2-(4-iodo-2-methylphenylamino)benzoic acid; 3,4,5-Trifluoro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid; 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid; 30 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid; 87 WO 00/35435 PCT/US99/29591
5-Chloro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid; Sodium 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-benzoate; 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-benzoic acid; 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-benzoic acid; 5 4-Chloro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid; 2-(4-Iodo-2-methyl-phenylamino)-benzoic acid; 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-benzoic acid; 5-Iodo-2-(4-iodo-2-methyl-phenylamino)-benzoic acid; 2,3,5-Trifluoro-4-(4-iodo-2-methyl-phenylamino)-benzoic acid; 10 2-(4-Iodo-phenylamino)-5-methoxy-benzoic acid; 5-Methyl-2-(4-iodo-2-methyl-phenylamino)-benzoic acid; 2-(4-Iodo-2-methyl-phenylamino)-4-nitro-benzoic acid; 2-(4-Bromo-2-methyl-phenylamino)-4-fluoro-benzoic acid; 2-(2-Bromo-4-iodo-phenylamino)-5-nitro-benzoic acid; 15 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-benzoic acid; 5-Chloro-N-(2-hydroxyethyl)-2-(4-iodo-2-methyl-phenylamino) benzamide; 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide; 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-methyl-benzamide; 20 N-Ethyl-4-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide; 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N,N-dimethyl benzamide; 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(1H-tetrazol-5-yl) benzamide; 25 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-benzamide; 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N,N-dimethyl benzamide; [5-Chloro-2-(4-iodo-2-methyl-phenylamino)-benzoylamino] -acetic acid; 30 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-propyl-benzamide; 5-Bromo-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino) benzamide; 88 WO 00/35435 PCT[US99/29591 N,N-Diethyl-4-fluoro-2-(4-iodo-2-methyl-phenylamnino) benzamide; 4-Fluoro-N- { 3-[4-(2-hydroxy-ethyl)-piperazin- 1 -yl]-propyl)} 2-(4-iodo-2-methyl-phenylamino)-benzamide; 5 N,N-Diethyl-2-(4-iodo-2-methyl-phenylamino)-5-nitro-benzamide; N-Butyl-4-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide; 5-Chloro-N,N-diethyl-2-(4-iodo-2-methyl-phenylamino) benzamide; 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N,N-dimethyl 10 benzamide; 5-Bromo-3 ,4-difluoro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl phenylamino)-benzamnide; N-(2,3 -Dihydroxy-propyl)-3 ,4-difluoro-2-(4-iodo-2-methyl phenylamnino)-benzamnide; 15 5-Bromo-3 ,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N (2-piperidin- 1 -yl-ethyl)-benzamide; 3 ,4-Difluoro-N-(2-hydroxy-ethyl)-2-(4-iodo-2 -methyl phenylamnino)-benzamide; N-(2,3-Dihydroxy-propyl)-4-fluoro-2-(4-iodo-2-methyl 20 phenylamino)-benzamide; 3 ,4-Difluoro-N-(3-hydroxy-propyl)-2-(4-iodo-2-methyl phenylamnino)-benzamnide; 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N (2-pyrrolidin- 1 -yl-ethyl)-benzamide; 25 5-Bromo-3 ,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N (2-pyridin-4-yl-ethyl)-benzamide; 4-Fluoro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino) benzamide; 5-Bromo-N-(3-dimethylamino-propyl)-3 ,4-difluoro-2-(4-iodo 30 2-methyl-phenylamnino)-benzamide; 5-Bromo-3 ,4-difluoro-2-(4-iodo-2-methyl-phenylamnino)-N (2-morpholin-4-yl-ethyl)-benzamide; 89 WO 00/35435 PCT1US99/2959 I 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-morpholin 4-yl-ethyl)-benzamide; 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamnino)-N-(2-pyrrolidin 1 -yl-ethyl)-benzamide; 5 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyridin-4-yl ethyl)-benzamnide; N-(3 -Dimethylamino-propyl)-3,4-difluoro-2-(4-iodo-2 -methyl phenylamnino)-benzamide; N-Benzyl-4-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide; 10 2-(4-Bromo-2-methyl-phenylamino)-3 ,4-difluoro-N-(2-hydroxy ethyl)-benzamnide; 4-Fluoro-2-(4-iodo-2-methyl-phenylamnino)-N-(2-morpholin-4-yl ethyl)-benzamide; 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3 -piperidin- l-yl 15 propyl)-benzamide; 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3-piperidin 1 -yl-propyl)-benzamnide; 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-thiophen-2-yl ethyl)-benzamide; 20 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyrrolidin- l-yl ethyl)-benzamide; 2-(4-Bromo-2-methyl-phenylamino)-3 ,4-difluoro-N-(2-morpholin 4-yl-ethyl)-benzamnide; 5-Bromo-3 ,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N 25 pyridin-4-ylmethyl-benzamide; 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-pyridin 4-ylmethyl-benzamide; 2-(4-Bromo-2-methyl-phenylamino)-N-(3-dimethylamino propyl)-3 ,4-difluoro-benzamide; 30 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-pyridin-4-ylmethyl benzamnide; 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyridin-4-yl ethyl)-benzamide; 90 WO 00/35435 PCTIUS99/29591 2-(4-Bromo-2-methyl-phenylamnino)-3 ,4-difluoro-N-(2-pyridin 4-yl-ethyl)-benzamide; 2-(4-Bromo-2-methyl-phenylamnino)-3 ,4-difluoro-N-(3-hydroxy propyl)-benzamnide; 5 2-(4-Bromo-2-methyl-phenylamino)-3 ,4-difluoro-N-(2-pyrrolidin 1 -yl-ethyl)-benzamide; 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-phenethyl benzamide; 2-(4-Bromo-2-methyl-phenylamino)-3,4-difluoro-N-(2-thiophen 10 2-yl-ethyl)-benzamide; 2-(4-Bromo-2-methyl-phenylamino)-3 ,4-difluoro-N-pyridin 4-ylmethyl-benzamide; 2-(4-Bromo-2-methyl-phenylamino)-3 ,4-difluoro-N-phenethyl benzamide; 15 2-(4-Bromo-2-methyl-phenylamino)-3 ,4-difluoro-N-(2-piperidin 1 -yl-ethyl)-benzamnide; 5-Chloro-N- { 3-[4-(2-hydroxy-ethyl)-piperazin- 1 -yl]-propyl} 2-(4-iodo-2-methyl- phenylamino)- benzamide; 5-Fluoro-N- {13- [4-(2-hydroxy-ethyl)-piperazin- Il-yl] -propyl } 20 2-(4-iodo-2-methyl- phenylamino)- benzamnide; 2-(4-Iodo-2-methyl-phenylamnino)-5-nitro-N-pyridin-4-y methyl benzamnide; 5-Bromo-N-1{3-[4-(2-hydroxy-ethyl)-piperazin- 1-yl]-propyl} 2-(4-iodo-2-methyl- phenylamino)- benzamide; 25 5-Chloro-N-(2-diethylamino-ethyl)-2-(4-iodo-2-methyl phenylamino)- benzamide; 5-Chloro-2-(4-iodo-2-methyl-phenylamnino)-N-(2-piperidin- l-yl ethyl)-benzamide; (3-Hydroxy-pyrrolidin- 1-yl)-[2-(4-iodo-2-methyl-phenylamino) 30 5-nitro-phenyl]-methanone; 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyrrolidin- Il-yl ethyl)-benzamide; 91 WO 00/35435 PCT/US99/29591 5-Bromo-N-(2-diethylamino-ethyl)-2-(4-iodo-2-methyl phenylamino)- benzamide; N- {2-[Bis-(2-hydroxy-ethyl)-amino]-ethyl }-5-chloro-2-(4-iodo 2-methyl- phenylamino)- benzamide; 5 N- {2-[Bis-(2-hydroxy-ethyl)-amino] -ethyl }-5-bromo-2-(4-iodo 2-methyl- phenylamino)- benzamide; N-{ 3-[4-(2-Hydroxy-ethyl)-piperazin-1-yl]-propyl}-2-(4-iodo 2-methyl-phenylamino)- benzamide; 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-pyridin-4-ylmethyl 10 benzamide; 5-Bromo-2-(4-iodo-2-ethyl-phenylamino)-N-(2-pyrrolidin-1-yl ethyl)-benzamide; 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(2-piperidin- 1-yl ethyl)-benzamide; 15 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-pyrrolidin-1-yl ethyl)-benzamide; 5-Chloro-N-(3-dimethylamino-propyl)-2-(4-iodo-2-methyl phenylamino)-benzamide; N- {2- [Bis-(2-hydroxy-ethyl)-amino] -ethyl} -5-fluoro-2-(4-iodo 20 2-methyl- phenylamino)- benzamide; 5-Chloro-N-(3-hydroxy-propyl)-2-(4-iodo-2-methyl-phenylamino) benzamide; 5-Chloro-N-(3-diethylamino-2-hydroxy-propyl)-2-(4-iodo 2-methyl-phenylamino)- benzamide; 25 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-piperidin-1 -yl ethyl)-benzamide; 5-Bromo-N-(3-hydroxy-propyl)-2-(4-iodo-2-methyl-phenylamino) benzamide; 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(3-piperidin-1 -yl 30 propyl)-benzamide; N- {2- [Bis-(2-hydroxy-ethyl)-amino]-ethyl } -2-(4-iodo-2-methyl phenylamino)-5-nitro- benzamide; 92 WO 00/35435 PCT/US99/29591 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-(2-morpholin-4-yl ethyl)-benzamide; 5-Chloro-N-(3-diethylamino-propyl)-2-(4-iodo-2-methyl phenylamino)-benzamide; 5 5-Chloro-N-(2-diisopropylamino-ethyl)-2-(4-iodo-2-methyl phenylamino)-benzamide; 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-(3-piperidin-1-yl propyl)-benzamide; 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-N-(2-piperidin- 1-yl 10 ethyl)-benzamide; 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(2-piperazin- 1-yl ethyl)-benzamide; N-(2-Diethylamino-ethyl)-5-fluoro-2-(4-iodo-2-methyl phenylamino)-benzamide; 15 5-Bromo-N-(3-dimethylamino-propyl)-2-(4-iodo-2-methyl phenylamino)-benzamide; N-(3-Hydroxy-propyl)-2-(4-iodo-2-methyl-phenylamino)-5-nitro benzamide; 5-Fluoro-N-(3-hydroxy-propyl)-2-(4-iodo-2-methyl-phenylamino) 20 benzamide; N-(3-Diethylamino-propyl)-5-fluoro-2-(4-iodo-2-methyl phenylamino)-benzamide; N-(3-Diethylamino-propyl)-2-(4-iodo-2-methyl-phenylamino) 5-nitro-benzamide; 25 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(2-morpholin-4-yl ethyl)-benzamide; 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-N-(3-piperidin-1-yl propyl)-benzamide; [5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-phenyl]-(3-hydroxy 30 pyrrolidin- 1 -yl)-methanone; 5-Bromo-N-(2-diisopropylamino-ethyl)-2-(4-iodo-2-methyl phenylamino)-benzamide; 93 WO 00/35435 PCTIUS99/29591 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-morpholin-4-y ethyl)-benzamide; 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3 -piperidin- 1l-yl propyl)-benzamide; 5 [5-Fluoro-2.-(4-iodo-2-methyl-phenylamino)-phenyl] [4-(2-hydroxy-ethyl)-piperazin- 1 -yl]-methanone; N-(3 -Diethylamnino-2-hydroxy-propyl)-5-fluoro-2-(4-iodo 2-methyl-phenylamino)- benzamide; N-Cyclopropyl-5-fluoro-2-(4-iodo-2-methyl-phenylamino) 10 benzamide; 5-Chloro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino) benzamide; 5-Fluoro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino) benzamide; 15 N-Benzyloxy-5-fluoro-2-(4-iodo-2-methyl-phenylamino) benzamide; N-Benzyloxy-5-bromo-2-(4-iodo-2-methyl-phenylamino) benzamide; 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-N-(4-sulfamoyl-benzyl) 20 benzamide; 5-Bromo-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino) benzamide; N-(2-Hydroxy-ethyl)-5-iodo-2-(4-iodo-2-methyl-phenylamino) benzamide; 25 N-(2-Hydroxy-ethyl)-2-(4-iodo-2-ethyl-phenylamino)-5 -nitro benzamide; 2-(4-Iodo-2-methyl-phenylamino)-N-methyl-5-nitro-N-phenyl benzamide; 5-Chloro-N-cyclopropyl-2-(4-iodo-2-methyl-phenylamino) 30 benzamide; 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-methyl-N-phenyl benzamide; N-Allyl-5-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide; 94 WO 00/35435 PCTIUS99/29591 N-Benzyloxy-5-iodo-2-(4-iodo-2-methyl-phenylamino) benzamnide; 5-Fluoro-2-(4-iodo-2-methyl-phenylamnino)-N-(4-sulfamoyl benzyl)-benzamide; 5 N-Allyl-5-chloro-2-(4-iodo-2-methyl-phenylamino)-benzamide; N-Cyclopropyl-2-(4-iodo-2-methyl-phenylamino)-5-nitro benzamide; 5-Bromo-N-cyclopropyl-2-(4-iodo-2-methyl-phenylamino) benzamide; 10 5-Chloro-2-(4-iodo-2-methyl-phenylamnino)-N-methyl-N-phenyl benzamide; 5-Iodo-2-(4-iodo-2-methyl-phenylamino)-N-(4-sulfamoyl-benzyl) benzamnide; 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(4-sulfamoyl 15 benzyl)-benzamide; N-Allyl-2-(4-iodo-2-methyl-phenylamnino)-5-nitro-benzamide; 2-(4-Iodo-2-methyl-phenylamino)-5-nitro-N-(4-sulfamoyl-benzyl) benzamide; N-Allyl-5 -bromo-2-(4-iodo-2-methyl-phenylamino)-benzamide; 20 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3-methyl-benzyl) benzamide; N-Cyclopropyl-5-iodo-2-(4-iodo-2-methyl-phenylamino) benzamide; 5-Bromo-2-(4-iodo-2-methyl-phenylamnino)-N-methyl-N-phenyl 25 benzamnide; N-Benzyloxy-2-(4-iodo-2-methyl-phenylamnino)-5 -nitro benzamide; N-Cyclohexyl-5-iodo-2-(4-iodo-2-methyl-phenylamino) benzamide; 30 N-Allyl-5-iodo-2-(4-iodo-2-methyl-phenylamino)-benzamide; 5-Iodo-2-(4-iodo-2-methyl-phenylamnino)-N-(3-methyl-benzyl) benzamide; 95 WO 00/35435 PCT/US99/29591 2-(4-Iodo-2-methyl-phenylamino)-N-(3 -methyl-benzyl)-5 -nitro benzamnide; 5-Iodo-2-(4-iodo-2-methyl-phenylamino)-N-methyl-N-phenyl benzamide; 5 N-Cyclohexyl-5-fluoro-2-(4-iodo-2-methyl-phenylamino) benzamnide; 5-Chloro-N-cyclohexyl-2-(4-iodo-2-methyl-phenylamino) benzamide; 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(3 -methyl-benzyl) 10 benzamnide; 5-Bromo-N-cyclohexyl-2-(4-iodo-2-methyl-phenylamino) benzamnide; 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-(3 -methyl-benzyl) benzamide; 15 N-Cyclohexyl-2-(4-iodo-2-methyl-phenylamino)-5-nitro benzamide; N-Benzyloxy-5-bromo-2-(4-iodo-2-methyl-phenylamnino) benzamide; N-Benzyloxy-5-fluoro-2-(4-iodo-2-methyl-phenylamino) 20 benzamide; 5-Chloro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino) benzamide; 5-Bromo-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino) benzamide; 25 2-(4-Iodo-2-methyl-phenylamino)-N-methyl-5-nitro-N-phenyl benzamide; 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-methyl-N-phenyl benzamnide; N-(2-Hydroxy-ethyl)-5-iodo-2-(4-iodo-2-methyl-phenylamino) 30 benzamide; 5-Chloro-N-cyclopropyl-2-(4-iodo-2-methyl-phenylamino) benzamnide; N-Allyl-5 -chloro-2-(4-iodo-2-methyl-phenylamino)-benzamide; 96 WO 00/35435 PCT/US99/29591I 5-Fluoro-2-(4-iodo-2-methyl-phenylamnino)-N-methyl-N-phenyl benzamide; N-(2-Hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino)-5 -nitro benzamide; 5 5 -Fluoro-N-(2-hydroxy-ethyl)-2-(4-iodo-2-methyl-phenylamino) benzamide; 5-Bromo-N-cyclopropyl-2-(4-iodo-2-methyl-phenylamino) benzamide; .s N-Cyclopropyl-5-fluoro-2-(4-iodo-2-methyl-phenylamino) 10 benzamide; 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(4-sulfamoyl benzyl)-benzamnide; N-Cyclopropyl-2-(4-iodo-2-methyl-phenylamnino)-5 -nitro benzamide; 15 N-Allyl-5-fluoro-2-(4-iodo-2-methyl-phenylamino)-benzamide; N-Benzyloxy-5-iodo-2-(4-iodo-2-methyl-phenylamnino) benzamnide; N-Allyl-5-bromo-2-(4-iodo-2-methyl-phenylamino)-benzamride; 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-(4-sulfamoyl 20 benzyl)-benzamide; 5-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-methyl-N-phenyl benzamnide; N-Allyl-2-(4-iodo-2-methyl-phenylamino)-5-nitro-benzamide; 4-Fluoro-2-(4-iodo-2-methyl-phenylamnino)-benzy alcohol; 25 [5-Chloro-2-(4-iodo-2-methyl-phenylamino)-phenyl] -methanol; [2-(4-Iodo-2-methyl-phenylamino)-5-nitro-phenyl] -methanol; [5-Bromo-2-(4-iodo-2-methyl-phenylamnino)-phenyl] -methanol; and N-Allyl-2-(4-iodo-2-methyl-phenylamino)-5-nitro-benzamide. 30 97 WO 00/35435 PCT/US99/29591
6. The method of claim 4, wherein the MEK inhibitor is a compound of Formula (I) wherein (a) R 1 is hydrogen, methyl, methoxy, fluoro, chloro, or bromo; (b) R 2 is hydrogen; (c) R 3 , R4, and R 5 independently are hydrogen, fluoro, chloro, bromo, iodo, methyl, methoxy, or nitro; (d) Rio 5 and R, I independently are hydrogen or methyl; (e) Z is COOR 7 , tetrazolyl, CONR 6 R 7 , CONHNRioR 1 , or CH 2 0R7; R 6 and R 7 independently are hydrogen, C 1.4 alkyl, heteroaryl, or C 3.5 cycloalkyl optionally containing one or two heteroatoms selected from 0, S, or NH; or R6 and R 7 together with the nitrogen to which they are attached complete a 5-6 member cyclic 10 ring optionally containing 1 or 2 additional heteroatoms selected from 0, NH or N-alkyl; and wherein any of the foregoing alkyl or aryl groups can be unsubstituted or substituted by halo, hydroxy, methoxy, ethoxy, or heteroaryloxy; (f) Z is COOR 7 ; (g) R7 is H, pentafluorophenyl, or tetrazolyl; (h) R 3 , R4, and R 5 are independently H, fluoro, or chloro; (i) R 4 15 is fluoro; (j) two of R 3 , R 4 , and R 5 are fluoro; or (k) combinations of the above.
7. The method of claim 6, wherein the MEK inhibitor is a compound of Formula (I) wherein: Z is COOR 7 ; R 7 is H, pentafluorophenyl, or tetrazolyl; R 3 and R 5 are independently H, fluoro, or chloro; and R 4 is 20 fluoro.
8. A method according to claim 1, where the MEK inhibitor is a compound of Formula II 0OR II I6a Rla f2a C-N-0- R7a Ria N 11 R5a Br or I R3a R4a 98 WO 00/35435 PCT/US99/29591 wherein: RIa is hydrogen, hydroxy, C 1 -C 8 alkyl, CI-C 8 alkoxy, halo, trifluoromethyl, or CN; R2a is hydrogen; 5 R3a, R4a, and R5a independently are hydrogen, hydroxy, halo, trifluoromethyl, CI-C 8 alkyl, Cl-C 8 alkoxy, nitro, CN, or (0 or NH)m-(CH2)n-R9a, where R9a is hydrogen, hydroxy, CO 2 H or NR1OaR11a n is 0-4; 10 m is 0 or 1; R10a and R 1 la independently are hydrogen or C 1 -C 8 alkyl, or taken together with the nitrogen to which they are attached can complete a 3- to 10-member cyclic ring optionally containing one, two, or three additional heteroatoms selected from 0, S, NH, or 15 N-CI-C 8 alkyl; R6a is hydrogen, C 1 -C 8 alkyl, (CO)-CI-C 8 alkyl, aryl, aralkyl, or C 3 -Cig cycloalkyl; R7a is hydrogen, CI-C 8 alkyl, C 2 -C 8 alkenyl, C 2 -C 8 alkynyl, C 3 -C 1 0 (cycloalkyl or cycloalkyl optionally containing a 20 heteroatom selected from 0, S, or NR9a); and wherein any of the foregoing alkyl, alkenyl, aryl, heteroaryl, heterocyclic, and alkynyl groups can be unsubstituted or substituted by halo, hydroxy, CI-C 6 alkoxy, amino, nitro, C 1 -C 4 alkylamino, di(Ci C 4 )alkylamino, C 3 -C 6 cycloalkyl, phenyl, phenoxy, C 3 -C 5 heteroaryl or 25 heterocyclic radical, or C 3 -C 5 heteroaryloxy or heterocyclic radical-oxy; or R6a and R7a taken together with the N to which they are attached can complete a 5- to 10-membered cyclic ring, optionally containing one, two, or three additional heteroatoms selected from 0, S, or NR 1 OaR 1 a; or a pharmaceutically acceptable salt, ester, amide or prodrug thereof. 30 99 WO 00/35435 PCT/US99/29591
9. The method of Claim 8, comprising a MEK inhibitor having a structure of Formula (II) wherein: (a) Ria is H, methyl, fluoro, or chloro; (b) R 2 a is H; R 3 a, R 4 a, and R 5 a are each H, Cl, nitro, or F; (c) R 6 a is H; (d) R 7 a is methyl, ethyl, 2-propenyl, propyl, butyl, pentyl, hexyl, cyclopropylmethyl, 5 cyclobutyl methyl, cyclopropylmethyl, or cyclopropylethyl; and (e) the 4' position is I, rather than Br.
10. The method of claim 9, comprising a MEK inhibitor having a structure of Formula (II) wherein: R 4 a is F at the 4 position, para to the CO-N-R 6 ca-OR 7 a 10 group and meta to the bridging nitrogen; at least one of R 3 a and R 5 a is F or Cl; and Ria is methyl or chloro.
11. The method of Claim 8, comprising a MEK inhibitor having a formula selected from: 4-Fluoro-N-hydroxy-2-(4-iodo-2-methyl-phenylamino)-benzamide; 15 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(methoxy) benzamide; 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(prop-2-ynyloxy) benzamide; 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-phenoxyethoxy) 20 benzamide; 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-thienylmethoxy) benzamide; 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(prop-2-enyloxy) benzamide; 25 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N (cyclopropylmethoxy)-benzamide; 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(cyclopentoxy) benzamide; 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N 30 (3-furylmethoxy)-benzamide; 3,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-ethoxy benzamide; 100 WO 00/35435 PCT/US99/29591 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(but-2-enyloxy) benzamide; 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(cyclopropyl methoxy)-benzamnide; 5 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-( 1 -methyiprop 2-ynyloxy)-benzamide; 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3 -phenyiprop 2-ynyloxy)-benzamnide; 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(3-methyl 10 5-phenylpent-2-en-4-ynyloxy)-benzamide; 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(prop 2-ynyloxy)-benzamide; 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(propoxy) benzamide; 15 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(cyclobutyloxy) benzamide; 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N (2-thienylmethoxy)-benzamnide; 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(2-methyl-prop 20 2-enyloxy)-benzamide; 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N (2-phenoxyethoxy)-benzamide; 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamnino)-N-(but-2-enyloxy) benzamide; 25 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(but-3 -ynyloxy) benzamide; 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamnino)-N (cyclopentyloxy)-benzamide; 3 ,4-Difluoro-2-(4-iodo-2-methyl-phenylamino)-N 30 (3 -(2-fluorophenyl)-prop-2-ynyloxy)-benzamide; 5-Bromo-3 ,4-difluoro-N-hydroxy-2-(4-iodo-2-methyl phenylamino)-benzamide; 101 WO 00/35435 PCT/US99/29591 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N (n-propoxy)-benzamide; 5-Bromo-3,4-difluoro-N-(furan-3-ylmethoxy)-2-(4-iodo-2-methyl phenylamino)-benzamide; 5 5-Bromo-N-(but-2-enyloxy)-3,4-difluoro-2-(4-iodo-2-methyl phenylamino)-benzamide 5-Bromo-N-butoxy-3,4-difluoro-2-(4-iodo-2-methyl phenylamino)-benzamide; 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N 10 (3-methyl-but-2-enyloxy)-benzamide; 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N (3-methyl-pent-2-en-4-ynyloxy)-benzamide; 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-benzyl)-N [5-(3-methoxy-phenyl)-3-methyl-pent-2-en-4-ynyloxy]-benzamide; 15 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N-(prop 2-ynyloxy)-benzamide; 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N [3-(3-methoxy-phenyl)-prop-2-ynyloxy]-benzamide; 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N 20 (thiopen-2-ylmethoxy)-benzamide; 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N (pyridin-3-ylmethoxy)-benzamide; 5-Bromo-3-4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N (3-(2-fluorophenyl)-prop-2-ynyloxy)-benzamide; 25 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N (ethoxy)-benzamide; 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N (cyclopropylmethoxy)-benzamide; 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N 30 (isopropoxy)-benzamide; 5-Bromo-3,4-difluoro-2-(4-iodo-2-methyl-phenylamino)-N-but 3-ynyloxy)-benzamide; 102 WO 00/35435 PCT/US99/29591 5-Chloro-N-hydroxy-2-(4-iodo-2-methyl-phenylamino) benzamide; 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-(tetrahydro-pyran 2-yloxy)-benzamide; 5 5-Chloro-2-(4-iodo-2-methyl-phenylamino)-N-methoxy benzamide; 4-Bromo-2-(4-iodo-2-methyl-phenylamino)-N-phenylmethoxy benzamide; 4-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-phenylmethoxy 10 benzamide; 5-Fluoro-N-hydroxy-2-(4-iodo-2-methyl-phenylamino)-benzamide; 5-Iodo-2-(4-iodo-2-methyl-phenylamino)-N-phenylmethoxy benzamide; 5-Fluoro-2-(4-iodo-2-methyl-phenylamino)-N-(tetrahydropyran 15 2-yloxy)-benzamide; 3,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N (3-phenylprop-2-ynyloxy)-benzamide; 3,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N (3-furylmethoxy)-benzamide; 20 3,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N (2-thienylmethoxy)-benzamide; 3,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N-(but 3-ynyloxy)-benzamide; 3,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N-(2-methyl 25 prop-2-enyloxy)-benzamide; 3,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N-(but 2-enyloxy)-benzamide; 3,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N-(methoxy) benzamide; 30 3,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N-(ethoxy) benzamide; 3,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N (cyclobutoxy)-benzamide; 103 WO 00/35435 PCT[US99/29591 3 ,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N-(isopropoxy) benzamide; 3 ,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N (2-phenoxyethoxy)-benzamide; 5 3 ,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N-(cyclopropyl methoxy)-benzamide; 3 ,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N-(n-propoxy) benzamide; 3 ,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N-( 1-methyl 10 prop-2-ynyloxy)-benzamnide; 3 ,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N (3-(3-fluorophenyl)-prop-2-ynyloxy)-benzamide; 3 ,4-Difluoro-2-(4-bromo-2-methyl-phenylamino)-N (4,4-dimethylpent-2-ynyloxy)-benzamide; 15 3 ,4-Difluoro-2-(4-bromo-2-methyl-phenylamnino)-N (cyclopentoxy)-benzamide; 3 ,4,5-Trifluoro-N-hydroxy-2-(4-iodo-2-methyl-phenylamino) benzamide; 5-Chloro-3 ,4-difluoro-N-hydroxy-2-(4-iodo-2-methyl 20 phenylamino)-benzamide; 5-Bromo-3,4-difluoro-2-(2-fluoro-4-iodo-phenylamino)-N hydroxy-benzamide; N-Hydroxy-2-(4-iodo-2-methyl-phenylamnino)-4-nitro-benzamide; 3 ,4,5-Trifluoro-2-(2-fluoro-4-iodo-phenylamino)-N-hydroxy 25 benzamide; 5-Chloro-3,4-difluoro-2-(2-fluoro-4-iodo-phenylamino)-N hydroxy-benzamnide; 5-Bromo-2-(2-.chloro-4-iodo-phenylamino)-3 ,4-difluoro-N hydroxy-benzamide; 30 2-(2-Fluoro-4-iodo-phenylamino)-N-hydroxy-4-nitro-benzamide; 2-(2-Chloro-4-iodo-phenylamino)-3 ,4,5-trifluoro-N-hydroxy benzamide; 104 WO 00/35435 PCT/US99/29591 5-Chloro-2-(2-chloro-4-iodo-phenylamino)-3,4-difluoro-N hydroxy-benzamide; 5-Bromo-2-(2-bromo-4-iodo-phenylamino)-3,4-difluoro-N hydroxy-benzamide; 5 2-(2-Chloro-4-iodo-phenylamnino)-N-hydroxy-4-methyl benzamide; 2-(2-Bromo-4-iodo-phenylamino)-3 ,4,5-trifluoro-N-hydroxy benzamnide; 2-(2-Bromo-4-iodo-phenylamnino)-5-chloro-3,4-difluoro-N 10 hydroxy-benzamnide; 2-(2-Bromo-4-iodo-phenylamino)-N-hydroxy-4-nitro-benzamide; 4-Fluoro-2-(2-fluoro-4-iodo-phenylamino)-N-hydroxy-benzamide; 3 ,4-Difluoro-2-(2-fluoro-4-iodo-phenylamino)-N-hydroxy benzamide; 15 2-(2-Chloro-4-iodo-phenylamnino)-4-fluoro-N-hydroxy-benzamide; 2-(2-Chloro-4-iodo-phenylamino)-3 ,4-difluoro-N-hydroxy benzamide; 2-(2-Bromo-4-iodo-phenylamino)-4-fluoro-N-hydroxy-benzamide; 2-(2-Bromo-4-iodo-phenylamnino)-3 ,4-difluoro-N-hydroxy 20 benzamide; N-Cyclopropylmethoxy-3 ,4,5-trifluoro-2-(4-iodo-2-methyl phenylamnino)-benzamnide; 5-Chloro-N-cyclopropylmethoxy-3 ,4-difluoro-2-(4-iodo-2-methyl phenylamino)-benzamide; 25 5-Bromo-N-cyclopropylmethoxy-3 ,4-difluoro-2-(2-fluoro-4-iodo phenylamnino)-benzamnide; N-Cyclopropylmethoxy-2-(4-iodo-2-methyl-phenylamino)-4-nitro benzamide; N-Cyclopropylmethoxy-3 ,4,5-trifluoro-2-(2-fluoro-4-iodo 30 phenylamino)-benzamide; 5-Chloro-N-cyclopropylmethoxy-3 ,4-difluoro-2-(2-fluoro-4-iodo phenylamino)-benzamide; 105 WO 00/35435 PCTIUS99/29591 5-Bromo-2-(2-chloro-4-iodo-phenylamino)-N cyclopropylmethoxy-3 ,4-difluoro-benzamide; N-Cyclopropylmethoxy-2-(2-fluoro-4-iodo-phenylamino)-4-nitro benzamide; 5 2-(2-Chloro-4-iodo-phenylamnino)-N-cyclopropylmethoxy 3 ,4,5-trifluoro-benzamide; 5-Chloro-2-(2-chloro-4-iodo-phenylamino)-N cyclopropylmethoxy-3 ,4-difluoro-benzamide; 5-Bromo-2-(2-bromo-4-iodo-phenylamino)-N-ethoxy-3 ,4-difluoro 10 benzamide; 2-(2-Chloro-4-iodo-phenylamino)-N-ethoxy-4-nitro-benzamide; 2-(2-Bromo-4-iodo-phenylamino)-N-cyclopropylmethoxy 3 ,4,5-trifluoro-benzamnide; 2-(2-Bromo-4-iodo-phenylamino)-5-chloro-N 15 cyclopropylmethoxy-3,4-difluoro-benzamide 2-(2-Bromo-4-iodo-phenylamino)-N-cyclopropylmethoxy-4-nitro benzamnide; N-Cyclopropylmethoxy-4-fluoro-2-(2-fluoro-4-iodo-phenylamino) benzamide; 20 N-Cyclopropylmethoxy-3,4-difluoro-2-(2-fluoro-4-iodo phenylamino)-benzamnide; 2-(2-Chloro-4-iodo-phenylamino)-N-cyclopropylmethoxy 4-fluoro-benzamide; 2-(2-Chloro-4-iodo-phenylamino)-N-cyclopropylmethoxy 25 3 ,4-difluoro-benzamide; 2-(2-Bromo-4-iodo-phenylamino)-N-cyclopropylmethoxy 4-fluoro-benzamide; and 2-(2-Bromo-4-iodo-phenylamino)-N-cyclopropylmethoxy 3 ,4-difluoro-benzamnide. 30
12. The method of claim 1, comprising a MEK inhibitor having a structure selected from: 106 WO 00/35435 PCT/US99/29591 2-(2-chloro-4-iodophenylamino)-5-chloro-N-cyclopropylmethoxy -3,4 difluorobenzamide (PD 297189); 2-(4-iodophenylamino)-N cyclopropylmethoxy-5-chloro-3,4-difluorobenzamide (PD 297190); 2-(4 iodophenylamino)-5-chloro-3,4-difluorobenzoic acid (PD 296771); 2-(2 5 chloro-4-iodophenylamino)-5-chloro-3,4-difluorobenzoic acid (PD 296770); 5-chloro-3,4-difluoro-2-(4-iodo-2-methylphenylamino) benzoic acid (PD 296767); and 5-chloro-N-cyclopropylmethoxy -3,4 difluoro-2-(4-iodo-2-methylphenylamino)-benzamide (PD 298127).
13. A method for preventing and controlling the rejection in a patient of a 10 transplanted organ, cell, tissue or limb, said method comprising the step of administering to the patient who has undergone a transplant, or who is scheduled to undergo a transplant, an effective immunosuppressive amount of a compound selected from: 2-(2-Chloro-4-iodophenylamino)-N-cyclopropylmethoxy 15 3,4-difluorobenzamide (PD 184352); 2-(2-Methyl-4-iodophenylamino)-N-hydroxy-4-fluorobenzamide (PD170611); 2-(2-Methyl-4-iodophenylamino)-N-hydroxy-3,4-difluoro 5-bromobenzamide (PD171984); 20 2-(2-Methyl-4-iodophenylamino)-N-cyclopropylmethoxy 3,4-difluoro-5-bromobenzamide (PD177168); 2-(2-Methyl-4-iodophenylamino)-N-cyclobutylmethoxy 3,4-difluoro-5-bromobenzamide (PD 180841); 2-(2-Chloro-4-iodophenylamino)-N-cyclopropylmethoxy 25 3,4-difluoro-5-bromobenzamide (PD 184161); 2-(2-Chloro-4-iodophenylamino)-N-hydroxy-3,4-difluoro 5-bromobenzamide (PD 184386); 2-(2-Chloro-4-iodophenylamino)-N-cyclobutylmethoxy 3,4-difluorobenzamide (PD 185625); 30 2-(2-Chloro-4-iodophenylamino)-N-hydroxy-4-fluorobenzamide (PD 185848); 107 WO 00/35435 PCT/US99/29591 2-(2-Methyl-4-iodophenylamino)-N-hydroxy 3,4-difluorobenzamide(PD 188563); 2-(2-Methyl-4-iodophenylamino)-N-cyclopropylmethoxy 3,4,5-trifluorobenzamide (PD 198306); and 5 2-(2-Chloro-4-iodophenylamino)-N-cyclopropylmethoxy 4-fluorobenzamide (PD 203311).
14. A method for the prophylaxis or maintenance of transplant rejection in a mammal comprising administering to a patient in need of prophylaxis or 10 maintenance an effective amount of 2-(2-chloro-4-iodophenylamino)-N cyclopropylmethoxy-3,4-difluorobenzamide.
15. A method for the prophylaxis or maintenance of transplant rejection in a mammal comprising administering to a patient in need of prophylaxis or maintenance an effective amount of 2-(2-methyl-4-iodophenylamino)-N 15 cyclopropylmethoxy-3,4,5-trifluorobenzamide. 108
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Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EE05450B1 (en) 2000-07-19 2011-08-15 Warner-Lambert Company Oxygenated esters of 4-iodophen laminobenzh droxamic acids, their crystalline forms and pharmaceutical compositions and their use
NZ518726A (en) 2001-05-09 2004-06-25 Warner Lambert Co Method of treating or inhibiting neutrophil chemotaxis by administering a mek inhibitor
MXPA06004363A (en) 2003-10-21 2006-06-14 Warner Lambert Co Polymorphic form of n-[(r)-2, 3-dihydroxy -propoxy]-3, 4-difluoro-2 -(2-fluoro-4 -iodophenylamino) -benzamide.
US7378423B2 (en) 2004-06-11 2008-05-27 Japan Tobacco Inc. Pyrimidine compound and medical use thereof
PL1761528T3 (en) 2004-06-11 2008-05-30 Japan Tobacco Inc 5-amino-2,4,7-trioxo-3,4,7,8-tetrahydro-2h-pyrido[2,3-d]pyrimidine derivatives and related compounds for the treatment of cancer
JP5129143B2 (en) 2005-10-07 2013-01-23 エグゼリクシス， インコーポレイテッド MEK inhibitor and method of use thereof
US8217042B2 (en) 2005-11-11 2012-07-10 Zentaris Gmbh Pyridopyrazines and their use as modulators of kinases
EP1790342A1 (en) 2005-11-11 2007-05-30 Zentaris GmbH Pyridopyrazine derivatives and their use as signal transduction modulators
WO2007054556A1 (en) 2005-11-11 2007-05-18 Æterna Zentaris Gmbh Novel pyridopyrazines and their use as modulators of kinases
CA2649913A1 (en) 2006-05-19 2007-11-29 Wyeth N-benzoyl- and n-benzylpyrrolidin-3-ylamines as histamine-3 antagonists
PL2101759T3 (en) 2006-12-14 2019-05-31 Exelixis Inc Methods of using mek inhibitors
ES2399384T3 (en) 2008-11-10 2013-04-01 Bayer Schering Pharma Ag Sulfonamido substituted phenoxybenzamides
WO2011047795A1 (en) 2009-10-21 2011-04-28 Bayer Schering Pharma Aktiengesellschaft Substituted benzosulphonamides
JP2013508318A (en) 2009-10-21 2013-03-07 バイエル・ファルマ・アクチェンゲゼルシャフト Substituted benzosulfonamide derivatives
US20120263714A1 (en) 2009-10-21 2012-10-18 Bayer Intellectual Property Gmbh Substituted halophenoxybenzamide derivatives
JP2013542214A (en) 2010-10-29 2013-11-21 バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング Substituted phenoxypyridines
TR201807861T4 (en) 2012-10-12 2018-06-21 Exelixis Inc New process for making compounds for use in the treatment of cancer.
WO2019210008A1 (en) 2018-04-24 2019-10-31 Translational Drug Development, Llc Amide compounds as kinase inhibitors, compositions and methods of treatment
WO2020142745A1 (en) * 2019-01-04 2020-07-09 Translational Drug Development, Llc Methods of treating graft versus host disease and neoplastic disease with amide compounds

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5155110A (en) * 1987-10-27 1992-10-13 Warner-Lambert Company Fenamic acid hydroxamate derivatives having cyclooxygenase and 5-lipoxygenase inhibition
US5624946A (en) * 1994-07-05 1997-04-29 Williams; James Use of leflunomide to control and reverse chronic allograft rejection
US5525625A (en) * 1995-01-24 1996-06-11 Warner-Lambert Company 2-(2-Amino-3-methoxyphenyl)-4-oxo-4H-[1]benzopyran for treating proliferative disorders
AU5259296A (en) * 1995-04-07 1996-10-23 Warner-Lambert Company Flavones and coumarins as agents for the treatment of athero sclerosis
US6251943B1 (en) * 1997-02-28 2001-06-26 Warner-Lambert Company Method of treating or preventing septic shock by administering a MEK inhibitor
PT993439E (en) * 1997-07-01 2004-12-31 Warner Lambert Co 4-BROMINE OR 4-IODOPHENYLAMINOBENZYDROXYM ACID DERIVATIVES AND THEIR USE AS MEK INHIBITORS
ES2274572T3 (en) * 1997-07-01 2007-05-16 Warner-Lambert Company Llc ACID DERIVATIVES 2- (4-BROMO- OR 4-IODO-PHENYLAMINE) BENZOIC AND ITS USE AS A MEK INHIBITOR.
AU1951499A (en) * 1998-01-06 1999-07-26 General Hospital Corporation, The Use of mek1 inhibitors as protective agents against damage due to ischemia

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