AU2021104636A4 - PREMIXING OF Bacillus subtilis IN CONCRETE TO SELF HEAL THE MICRO CRACKS AND ENHANCE ITS COMPRESSIVE STRENGTH - Google Patents
PREMIXING OF Bacillus subtilis IN CONCRETE TO SELF HEAL THE MICRO CRACKS AND ENHANCE ITS COMPRESSIVE STRENGTH Download PDFInfo
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- AU2021104636A4 AU2021104636A4 AU2021104636A AU2021104636A AU2021104636A4 AU 2021104636 A4 AU2021104636 A4 AU 2021104636A4 AU 2021104636 A AU2021104636 A AU 2021104636A AU 2021104636 A AU2021104636 A AU 2021104636A AU 2021104636 A4 AU2021104636 A4 AU 2021104636A4
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- 239000004567 concrete Substances 0.000 title claims abstract description 124
- 244000063299 Bacillus subtilis Species 0.000 title claims description 7
- 235000014469 Bacillus subtilis Nutrition 0.000 title claims description 7
- 235000008113 selfheal Nutrition 0.000 title description 5
- 244000179560 Prunella vulgaris Species 0.000 title description 3
- 238000000034 method Methods 0.000 claims abstract description 49
- 230000001580 bacterial effect Effects 0.000 claims abstract description 39
- 239000000203 mixture Substances 0.000 claims abstract description 31
- 238000002360 preparation method Methods 0.000 claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 230000035876 healing Effects 0.000 claims abstract description 15
- 229960002401 calcium lactate Drugs 0.000 claims abstract description 8
- 239000001527 calcium lactate Substances 0.000 claims abstract description 8
- 235000011086 calcium lactate Nutrition 0.000 claims abstract description 8
- 239000004568 cement Substances 0.000 claims abstract description 8
- 239000004576 sand Substances 0.000 claims abstract description 8
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 claims abstract description 7
- 235000015097 nutrients Nutrition 0.000 claims abstract description 5
- 238000012258 culturing Methods 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims abstract description 4
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 10
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 5
- 229910021532 Calcite Inorganic materials 0.000 claims description 4
- 239000000654 additive Substances 0.000 claims description 3
- 230000000996 additive effect Effects 0.000 claims description 3
- 230000008021 deposition Effects 0.000 claims description 3
- 230000008439 repair process Effects 0.000 abstract description 3
- 239000002609 medium Substances 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 10
- 230000008901 benefit Effects 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 230000000052 comparative effect Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000005336 cracking Methods 0.000 description 3
- 230000002503 metabolic effect Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 241001529739 Prunella <angiosperm> Species 0.000 description 2
- 230000002308 calcification Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- 101000965313 Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) Aconitate hydratase A Proteins 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000033558 biomineral tissue development Effects 0.000 description 1
- 239000004566 building material Substances 0.000 description 1
- 229960005069 calcium Drugs 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 125000003700 epoxy group Chemical group 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B28/00—Compositions of mortars, concrete or artificial stone, containing inorganic binders or the reaction product of an inorganic and an organic binder, e.g. polycarboxylate cements
- C04B28/02—Compositions of mortars, concrete or artificial stone, containing inorganic binders or the reaction product of an inorganic and an organic binder, e.g. polycarboxylate cements containing hydraulic cements other than calcium sulfates
- C04B28/04—Portland cements
-
- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B2103/00—Function or property of ingredients for mortars, concrete or artificial stone
- C04B2103/0001—Living organisms, e.g. microorganisms, or enzymes
-
- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B2111/00—Mortars, concrete or artificial stone or mixtures to prepare them, characterised by specific function, property or use
- C04B2111/20—Resistance against chemical, physical or biological attack
- C04B2111/2038—Resistance against physical degradation
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Ceramic Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Materials Engineering (AREA)
- Structural Engineering (AREA)
- Organic Chemistry (AREA)
- Materials For Medical Uses (AREA)
Abstract
The present invention generally relates to a method for preparation of a self
healing concrete that also enhances the compressive strength. The method
comprises culturing a bacterial strain in a nutrient medium to get a bacterial
culture, mixing the bacterial culture into a liquid medium to get a bacterial
medium. The method further comprises mixing cement, natural sand, a
coarse aggregate and water to get a concrete mixture, which is then mixed
with the bacterial medium and calcium lactate to get a self-healing concrete
mixture. The method further comprises setting the self-healing concrete
mixture for a predetermined amount of time to get a self-healing concrete
and later micro-cracks were induced in it. Lastly, the method further
comprises curing the self-healing concrete that results in automatic repair of
one or more cracks and helps in maintaining the integrity, strength and
durability of the self-healing concrete.
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Description
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PREMIXING OF Bacillus subtilis IN CONCRETE TO SELF HEAL
The present invention relates to the use of bacteria in concrete for self healing the micro cracks and enhance the strength and durability of the concrete. BACKGROUND OF THE INVENTION
Concrete is the most essential building material and its demand is ever increasing with the exponential growth of infrastructure and development. However, even micro cracks in concrete, have a very detrimental effect on concrete's strength. A lot of research is done for the development of special concretes which have increased strength, durability and are sustainable for a long period of time. Despite these advancements, cracking still remains a key concern. Various materials like epoxies and sealing agents are used to repair these cracks. Nonetheless, they are not economical, as they require constant maintenance and also look unaesthetic. Therefore in order to overcome the inadequacy of conventional sealing agents, self healing concrete is required, which restores the micro cracks and also aid in improving the strength of structure. In the view of the forgoing discussion, it is clearly portrayed that there is a need to have a method for preparation of a self-healing concrete, which can not only self-heal the micro-cracks in concrete but also enhances the compressive strength of the concrete as compared to the plain concrete. The proposed invention aims to provide self-healing of micro-cracks which will reduce porosity and permeability of the concrete, thereby increasing load capacity of the material. Additionally, such a material would not involve any external agents for repairing the cracks.
The present disclosure seeks a method for preparation of a self-healing concrete. Plain concrete has very low tensile strength, ductility and little resistance to cracking. Cracking is a result of shrinkage and tensile stresses, which cannot be entirely avoided. Major cracks which are greater than 2mm are risk zones as they may cause failure of the structure. But even, micro cracks which are less than 2mm are potentially dangerous causing widening of cracks overtime. This makes the concrete weaker in strength and ultimately reduces the life of the structure. The present invention introduces a bio- mineralization technique also known as Microbiologically Induced Calcite or Calcium Carbonate (CaCO 3 ) Precipitation (MICP) to automatically repair micro cracks. Application of this technique in concrete has led to a new innovation known as Bacterial Concrete or Self-Healing concrete.
In an embodiment, the method for preparation of a self-healing concrete comprises culturing a bacterial strain in a nutrient medium to get a bacterial culture. The method further comprises mixing the bacterial culture into a liquid medium to get a bacterial medium. The method further comprises mixing cement, natural sand, a coarse aggregate and water to get a concrete mixture. Here, cement is about 16. 2 5% by weight of the concrete mixture. Natural sand is about 16. 2 5% by weight of the concrete mixture. The coarse aggregate is about 3 2 .5% by weight of the concrete mixture and water is about 3 5% by weight of the concrete mixture. The method further comprises mixing the bacterial medium and calcium lactate to the concrete mixture to get a self-healing concrete mixture. The method further comprises setting the self-healing concrete mixture for a predetermined amount of time to get a self-healing concrete. Lastly, the method further comprises curing the self-healing concrete for a predetermined amount of time that results in a self-healing of one or more cracks on the self-healing concrete's surface and improved compressive strength of the self-healing concrete.
In another embodiment, the bacterial strain used in the method for preparation of a self-healing concrete is a strain of Bacillus subtilis.
In another embodiment, the bacterial medium used in the method for preparation of a self-healing concrete has a concentration of about 2.5x10 7 cells/ml.
In another embodiment, the coarse aggregate used in the method for preparation of a self-healing concrete is gravel.
In another embodiment, in the method for preparation of a self-healing concrete an additive is added to the concrete mixture to increase compressive strength of the self healing concrete.
In another embodiment, in the method for preparation of a self-healing concrete, the self-healing of one or more cracks is characterized by a calcite or calcium carbonate deposition in the one or more cracks, that is induced with ingress of water and air through the one or more cracks and wherein the water has a predetermined pH and a predetermined temperature.
In another embodiment, self-healing concrete, self-heals micro cracks having a depth of less than 2mm.
To further clarify advantages and features of the present disclosure, a more particular description of the invention will be rendered by reference to specific embodiments thereof, which is illustrated in the appended drawings. It is appreciated that these drawings depict only typical embodiments of the invention and are therefore not to be considered limiting of its scope. The invention will be described and explained with additional specificity and detail with the accompanying drawings.
These and other features, aspects, and advantages of the present disclosure will become better understood when the following detailed description is read with reference to the accompanying drawings in which like characters represent like parts throughout the drawings, wherein:
Figure 1 illustrates a flow chart of the method for preparation of a self-healing concrete in accordance with an embodiment of the present disclosure.
Figure 2 illustrates comparative images depicting self-healing in plain concrete and the self-healing during the testing phase.
Figure 3 illustrates images depicting intermediate stages of calcification during the testing phase.
Figure 4 illustrates a comparative graph depicting compressive strength in plain concrete and in the self-healing concrete as noted during the testing phase.
Further, skilled artisans will appreciate that elements in the drawings are illustrated for simplicity and may not have necessarily been drawn to scale. For example, the flow charts illustrate the method in terms of the most prominent steps involved to help to improve understanding of aspects of the present disclosure. Furthermore, in terms of the construction of the device, one or more components of the device may have been represented in the drawings by conventional symbols, and the drawings may show only those specific details that are pertinent to understanding the embodiments of the present disclosure so as not to obscure the drawings with details that will be readily apparent to those of ordinary skill in the art having benefit of the description herein. DETAILED DESCRIPTION
For the purpose of promoting an understanding of the principles of the invention, reference will now be made to the embodiment illustrated in the drawings and specific language will be used to describe the same. It will nevertheless be understood that no limitation of the scope of the invention is thereby intended, such alterations and further modifications in the illustrated system, and such further applications of the principles of the invention as illustrated therein being contemplated as would normally occur to one skilled in the art to which the invention relates.
It will be understood by those skilled in the art that the foregoing general description and the following detailed description are exemplary and explanatory of the invention and are not intended to be restrictive thereof.
Reference throughout this specification to "an aspect", "another aspect" or similar language means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present disclosure. Thus, appearances of the phrase "in an embodiment", "in another embodiment" and similar language throughout this specification may, but do not necessarily, all refer to the same embodiment.
The terms "comprises", "comprising", or any other variations thereof, are intended to cover a non-exclusive inclusion, such that a process or method that comprises a list of steps does not include only those steps but may include other steps not expressly listed or inherent to such process or method. Similarly, one or more devices or sub-systems or elements or structures or components proceeded by "comprises...a" does not, without more constraints, preclude the existence of other devices or other sub systems or other elements or other structures or other components or additional devices or additional sub-systems or additional elements or additional structures or additional components.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The system, methods, and examples provided herein are illustrative only and not intended to be limiting.
Embodiments of the present disclosure will be described below in detail with reference to the accompanying drawings.
Figure 1 illustrates a flow chart of the method for preparation of a self-healing concrete in accordance with an embodiment of the present disclosure.
The method 100 for preparation of a self-healing concrete comprises culturing 102 a bacterial strain in a nutrient medium to get a bacterial culture. The method 100 further comprises mixing 104 the bacterial culture into a liquid medium to get a bacterial medium. The method 100 further comprises mixing cement, natural sand, a coarse aggregate and water 106 to get a concrete mixture. Here, cement is about 16. 2 5% by weight of the concrete mixture. Natural sand is about 16. 2 5% by weight of the concrete mixture. The coarse aggregate is about 3 2 .5% by weight of the concrete mixture and water is about 3 5% by weight of the concrete mixture. The method 100 further comprises mixing the bacterial medium and calcium lactate 108 to the concrete mixture to get a self-healing concrete mixture. The method 100 further comprises setting 110 the self-healing concrete mixture for a predetermined amount of time to get a self-healing concrete and after making concrete cubes, micro cracks were induced in plain concrete cubes as well as bacterial cubes. Lastly, the method 100 further comprises curing the self-healing concrete 112 for a predetermined amount of time that results in a self-healing of one or more cracks on the self healing concrete's surface and improved compressive strength of the self healing concrete.
This technique of microbiological self healing by bacteria helps in combating the damage caused by the cracks. Self-healing concrete produces a calcium carbonate precipitate by metabolic activity of the bacteria.
The first step in preparation of the bacterial medium is counting the number of colonies in the bacterial culture. The required amount of bacteria for cube casting is calculated by given formula
Concentration of bacteria = Number of colonies / (Dilution x Amount plated)
In another embodiment, the bacterial strain used in the method for preparation of a self-healing concrete is a strain of Bacillus subtilis.
In another embodiment, the bacterial medium used in the method for preparation of a self-healing concrete has a concentration of about 2.5x10 7 cells/ml.
In another embodiment, the coarse aggregate used in the method for preparation of a self-healing concrete is gravel.
In another embodiment, in the method for preparation of a self-healing concrete an additive is added to the concrete mixture to increase compressive strength of the self healing concrete.
In another embodiment, in the method for preparation of a self-healing concrete, the self-healing of one or more cracks is characterized by a calcite or calcium carbonate deposition in the one or more cracks, that is induced with ingress of water and air through the one or more cracks and wherein the water has a predetermined pH and a predetermined temperature.
When a concrete structure is damaged all it requires is a little curing so that bacteria become activated and self healing process gets started. The self healing concrete can be cured with normal water. However, temperature and pH need to be maintained at desired level so as to increase the rate of metabolic activity of bacteria. With the ingress of water through these cracks the bacteria starts growing. It starts feeding in the nutrient - calcium lactate. Calcium lactate is converted to calcium precipitate which then congeals on the cracked surface, thereby sealing it up.
In another embodiment, the method for preparation of a self-healing concrete self-heals micro cracks having a depth of less than 2mm.
Figure 2 illustrates comparative images depicting self-healing in plain concrete and the self-healing during the testing phase in bacterial concrete.
Figure 3 illustrates images depicting intermediate stages of calcification during the testing phase.
Figure 4 illustrates a comparative graph depicting compressive strength in plain concrete and the self-healing concrete as noted during the testing phase.
For testing the self-healing ability and compressive strength of a self healing concrete, a comparative study was conducted between plain concrete and the self-healing concrete. Two sets of concrete cubes were prepared- Plain concrete cubes and concrete cubes with Calcium lactate and Bacillus subtilis, (referred in the study as bacterial concrete). Bacillus subtilis was cultured on Petri dishes with nutrient agar and kept in incubator at 10-12 dilution. The concentration was kept at 2.5x10 7 cells/ml. The concrete mix was prepared using cement, sand and coarse aggregate in ratio 1:1:2 and 3 5% water by weight. All cubes were cured in normal water with a temperature maintained at 230 C. The temperature was maintained to activate the bacteria in the bacterial concrete and increase their metabolic activities. The pH of water was maintained at above 8.5. It was checked at regular interval to ensure it was alkaline enough for bacteria to sustain and grow.
The strengths of various cubes were tested by compression testing machine at 7th 1 4 th and 2 8 th day. An average reading of three cubes was taken for both the concretes i.e. a total of 24 cubes was prepared. The concretes were also tested for workability.
To test the self healing property of concrete, micro cracks were induced in plain concrete cubes as well as bacterial concrete cubes. The bacterial concrete cubes were cured for a day in calcium lactate solution and then were left to dry. The cracks were monitored visually for both cubes. Fig 2 and 3 illustrate the images captured during the self healing process, which in our case extended for 28 days.
The comparative compressive strength (N/mm 2 ) of various cubes at 7th, 1 4 th
and 2 8 th day is depicted in Fig 4. From the figure, it was observed that there is a gradual increase in the compressive strength of the bacterial cubes. The highest compressive strength of the bacterial cube was measured on the 2 8 th day. Here, the percentage increase was found to be approximately 70% higher than the plain concrete cubes. The workability of bacterial concrete measured by slump value was not hampered. No healing was observed in plain concrete cube.
The drawings and the forgoing description give examples of embodiments. Those skilled in the art will appreciate that one or more of the described elements may well be combined into a single functional element. Alternatively, certain elements may be split into multiple functional elements. Elements from one embodiment may be added to another embodiment. For example, orders of processes described herein may be changed and are not limited to the manner described herein. Moreover, the actions of any flow diagram need not be implemented in the order shown; nor do all of the acts necessarily need to be performed. Also, those acts that are not dependent on other acts may be performed in parallel with the other acts. The scope of embodiments is by no means limited by these specific examples. Numerous variations, whether explicitly given in the specification or not, such as differences in structure, dimension, and use of material, are possible. The scope of embodiments is at least as broad as given by the following claims.
Benefits, other advantages, and solutions to problems have been described above with regard to specific embodiments. However, the benefits, advantages, solutions to problems, and any component(s) that may cause any benefit, advantage, or solution to occur or become more pronounced are not to be construed as a critical, required, or essential feature or component of any or all the claims.
Claims (7)
1. A method for preparation of a self-healing concrete comprising: culturing a bacterial strain in a nutrient medium to get a bacterial culture;
mixing the bacterial culture into a liquid medium to get a bacterial medium;
mixing cement, natural sand, a coarse aggregate and water to get a concrete mixture, wherein cement is about 16. 2 5% by weight of the concrete mixture, wherein natural sand is about 16. 2 5% by weight of the concrete mixture, wherein the coarse aggregate is about 3 2 .5% by weight of the concrete mixture and wherein water is about 3 5% by weight of the concrete mixture;
mixing the bacterial medium and calcium lactate to the concrete mixture to get a self-healing concrete mixture;
setting the self-healing concrete mixture for a predetermined amount of time to get a self-healing concrete; after making concrete cubes, micro cracks were induced in plain concrete cubes as well as bacterial cubes; and
curing the self-healing concrete for a predetermined amount of time, that results in a self-healing of one or more cracks on the self-healing concrete's surface and improved compressive strength of the self-healing concrete.
2. The method for preparation of a self-healing concrete of claim 1, wherein the bacterial strain is a strain of Bacillus subtilis.
3. The method for preparation of a self-healing concrete of claim 1 and 2, wherein the bacterial medium has a concentration of about 2.5x10 7 cells/ml.
4. The method for preparation of a self-healing concrete of claim 1, wherein the coarse aggregate is gravel.
5. The method for preparation of a self-healing concrete of claim 1, wherein an additive is added to the concrete mixture to increase compressive strength of the self healing concrete.
6. The method for preparation of a self-healing concrete of claim 1, wherein the self-healing of one or more cracks is characterized by a calcite or calcium carbonate deposition in the one or more cracks, that is induced with ingress of water and air through the one or more cracks and wherein the water has a predetermined pH and a predetermined temperature.
7. The method for preparation of a self-healing concrete of claim 1 and 6, wherein the one or more cracks are micro cracks having a depth of less than 2mm.
Figure 2
Figure 3
Figure 4
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN115872648A (en) * | 2022-12-05 | 2023-03-31 | 深圳大学 | Self-control trigger type self-repairing aggregate, preparation method thereof and coastal self-repairing concrete |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN115872648A (en) * | 2022-12-05 | 2023-03-31 | 深圳大学 | Self-control trigger type self-repairing aggregate, preparation method thereof and coastal self-repairing concrete |
CN115872648B (en) * | 2022-12-05 | 2024-03-19 | 深圳大学 | Self-control triggering type self-repairing aggregate, preparation method thereof and coastal self-repairing concrete |
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