AU2021100324A4 - A Prawn Recirculating Aquaculture Method Combined with Nitrification and Assimilation - Google Patents
A Prawn Recirculating Aquaculture Method Combined with Nitrification and Assimilation Download PDFInfo
- Publication number
- AU2021100324A4 AU2021100324A4 AU2021100324A AU2021100324A AU2021100324A4 AU 2021100324 A4 AU2021100324 A4 AU 2021100324A4 AU 2021100324 A AU2021100324 A AU 2021100324A AU 2021100324 A AU2021100324 A AU 2021100324A AU 2021100324 A4 AU2021100324 A4 AU 2021100324A4
- Authority
- AU
- Australia
- Prior art keywords
- water
- aquaculture
- culture
- nitrification
- assimilation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000009360 aquaculture Methods 0.000 title claims abstract description 50
- 244000144974 aquaculture Species 0.000 title claims abstract description 50
- 241000238557 Decapoda Species 0.000 title claims abstract description 25
- 230000003134 recirculating effect Effects 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 81
- 241000894006 Bacteria Species 0.000 claims abstract description 21
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 13
- 241000238553 Litopenaeus vannamei Species 0.000 claims abstract description 12
- 230000001954 sterilising effect Effects 0.000 claims abstract description 12
- 230000001580 bacterial effect Effects 0.000 claims abstract description 8
- 235000021552 granulated sugar Nutrition 0.000 claims abstract description 6
- 230000001546 nitrifying effect Effects 0.000 claims abstract description 6
- 239000013535 sea water Substances 0.000 claims abstract description 6
- 238000010586 diagram Methods 0.000 claims abstract description 4
- 239000003643 water by type Substances 0.000 claims abstract description 4
- 230000003203 everyday effect Effects 0.000 claims abstract description 3
- 235000013379 molasses Nutrition 0.000 claims abstract description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 20
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims description 12
- 229910021529 ammonia Inorganic materials 0.000 claims description 10
- 238000005273 aeration Methods 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- 241000607598 Vibrio Species 0.000 claims description 5
- 230000002354 daily effect Effects 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 210000003608 fece Anatomy 0.000 claims description 4
- 230000002070 germicidal effect Effects 0.000 claims description 4
- 239000006041 probiotic Substances 0.000 claims description 4
- 235000018291 probiotics Nutrition 0.000 claims description 4
- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 claims description 3
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 3
- 239000007844 bleaching agent Substances 0.000 claims description 3
- 239000000460 chlorine Substances 0.000 claims description 3
- 229910052801 chlorine Inorganic materials 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims description 3
- 239000010865 sewage Substances 0.000 claims description 3
- 241000589963 Planctomycetaceae Species 0.000 claims description 2
- 230000032770 biofilm formation Effects 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 230000007423 decrease Effects 0.000 claims description 2
- 239000004576 sand Substances 0.000 claims description 2
- 230000001965 increasing effect Effects 0.000 abstract description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 6
- 229910052799 carbon Inorganic materials 0.000 abstract description 6
- 241000251468 Actinopterygii Species 0.000 abstract description 5
- 230000000813 microbial effect Effects 0.000 abstract description 4
- 230000002708 enhancing effect Effects 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract description 2
- 230000002503 metabolic effect Effects 0.000 abstract 1
- 230000008901 benefit Effects 0.000 description 5
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 244000005700 microbiome Species 0.000 description 4
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 3
- 238000005265 energy consumption Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 241001430267 Rhodobacteraceae Species 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 241001248480 Alteromonadaceae Species 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000107884 Colwelliaceae Species 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000244332 Flavobacteriaceae Species 0.000 description 1
- 241000927735 Penaeus Species 0.000 description 1
- 241000107883 Pseudoalteromonadaceae Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002816 gill Anatomy 0.000 description 1
- 239000012774 insulation material Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000009364 mariculture Methods 0.000 description 1
- 238000004172 nitrogen cycle Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000009287 sand filtration Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/042—Introducing gases into the water, e.g. aerators, air pumps
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/045—Filters for aquaria
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Water Supply & Treatment (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a prawn recirculating aquaculture method combined with
nitrification and assimilation, belonging to the technical field of aquaculture. The
aquaculture system specifically comprises multiple culture ponds, a microfilter, a water
circulating pump, a biofilter, an ultraviolet sterilization device and a Roots blower. When
in use, fish bait of 40-50% brown granulated sugar or 50-60% molasses is added to the
pond every day to promote the growth of heterotrophic bacteria, and some metabolic
wastes are removed by bacterial assimilation. In the recirculating aquaculture system with
nitrification as the core of water treatment, carbon sources are continuously increased
during the cultivation, so that while promoting the growth of bacteria in the aquaculture
waters and enhancing the assimilation of bacteria, the nitrification of nitrifying bacteria is
not inhibited. The assimilation and nitrification together purify the water, thereby
improving the microbial water treatment efficiency, reducing the load of the water
treatment system and improving the aquaculture environment.
-1/2
.r PoUo"F culture cuure1
biofilTers e icrurofilter
Spnde poe pde pond
backwash
blowdown<'
and < coastal
reservoir< filter t seawater<
Figure I
The schematic structural diagram of the recirculating aquaculture method of Penaeus
vannamei.
Description
-1/2
.r PoUo"F culture cuure1 biofilTers e icrurofilter Spnde poe pde pond
backwash blowdown<'
and < coastal reservoir< filter t seawater<
Figure I
The schematic structural diagram of the recirculating aquaculture method of Penaeus
vannamei.
A Prawn Recirculating Aquaculture Method Combined with Nitrification and
Assimilation
The invention belongs to the technical field of aquaculture, and particularly relates to a
prawn recirculating aquaculture method combined with nitrification and assimilation.
Penaeus vannamei is one of the three major shrimp species in the world, which has the
advantages of fast growth, strong environmental adaptability and long survival time out
of water. In 2018, the mariculture yield of Penaeus vannamei in China was 1.118 million
tons, reaching 65.6% of that of crustaceans. Affected by the deterioration of ecological
environment and the high breeding density, prawn breeding industry is facing various
environmental pressures, which are not affected by unfavourable factors such as seasons
and weather. Not affected by season, weather and other adverse factors, the industrial
breeding mode with controllable environment is increasingly favoured by the industry,
especially in the northern region of China, where the industrial breeding mode of prawn
has developed to a certain extent. However, the industrial breeding of prawn is still
dominated by water exchanging mode, and the daily water exchange rate reaches
~70% in the later stage. On the one hand, high water exchange rate will further worsen
the surrounding water environment; on the other hand, it will also increase the risk of
contamination of aquaculture environment by external pathogens. Recirculating
aquaculture is a sustainable development mode, which comprehensively treats the aquaculture water through physical filtration, biological filtration, and disinfection to maintain a good aquaculture water environment, thereby realizing the recycling of aquaculture water, greatly reducing water consumption, and even get zero water exchange. Recirculating aquaculture has been developing for a long time in fish culture, and it is becoming more and more mature, but there is less application research in prawn culture field. Because there are some differences between shrimp culture and fish culture, there is no stable recirculating aquaculture system and technology at present.
Generally, the fish recirculating aquaculture system adopts a high water circulation rate,
which discharges solid particles such as residual bait and feces out of the system in time
to avoid clogging fish gills and deteriorating water quality, and uses nitrification of
biofilter to remove harmful substances such as ammonia and nitrite to maintain a good
water environment. Therefore, the water circulation rate of the system generally exceeds
times/day, and the energy consumption of system operation is very high. Moreover, in
order to ensure the aquaculture water quality, the biofilter has a large volume, which
generally accounts for 1/2-1/1 of the water in the aquaculture pond. Penaeus vannamei
has strong tolerance to suspended solid particles, total ammonia nitrogen, nitrite and other
water quality indicators, so it is usually cultivated in muddy water with more suspended
particles, and the 96 h safe concentrations of ammonium and nitrite in seawater reach 3.9
mg/L and 20.5 mg/L, respectively. Therefore, it is unnecessary and a waste of resources
for Penaeus vannamei culture to maintain strict water quality by adopting excessive
water circulation rate and excessive biofilter. Moreover, under high water circulation rate,
the water body is clear, and the abundance and diversity of microorganisms are low. In
addition, due to the role of sterilization device, the microbial community in the water is unstable, which easily causes prawn death and reduces the success rate of culture.
Although solid particles such as residual bait and feces in aquaculture system have the
risk of spoilage and deterioration of water quality, they can also promote the growth of
heterotrophic microorganisms, provide attachment growth space for microorganisms, and
absorb part of ammonia and nitrite through the assimilation of microorganisms, thus
realizing the function of water purification.
The invention provides a prawn recirculating aquaculture method combined with
nitrification and assimilation, so as to solve the technical problems existing in the prior
art, that is, low success rate of the recirculating aquaculture, high energy consumption of
system operation and low economic benefit.
The invention is realized according to the following technical scheme.
A prawn recirculating aquaculture method combined with nitrification and assimilation,
characterized in that its aquaculture system specifically comprises multiple culture ponds,
a microfilter, a water-circulating pump, a biofilter, an ultraviolet sterilization device and a
Roots blower. Wherein, the culture pond has a conical bottom with a slope of 1/15~1/20,
and the central blowdown method is adopted with the blowdown pipe shared with the
circulating backwater pipe. Nano-air pipes are arranged around the culture pond and fixed
at the bottom of the pond to push the water to rotate while inflating, which is convenient
for collecting pollution. The microflter is belt type or drum type, and the screen
specification is 100~120 meshes. The water-circulating pump is frequency conversion
type, which is convenient to control the flow rate. The volume of the biofilter is 1/5~1/4 of that of the aquaculture waters, and the biofilter is filled with brush medium or porous suspended medium, wherein, the filling rate of brush medium is 40-50% and porous suspended medium is 20~30%. The power of the ultraviolet germicidal lamp is 0.5~1.0 kw/100m 3. It is not necessary to start ultraviolet sterilization in the early stage of culture to promote the biofilm formation. When the number of Vibrio exceeds 8000 CFU/mL, the ultraviolet germicidal lamp is started for sterilization. The power of Roots blower is
0.7~1.0 kW/100m 2 . The biofilter is inflated by an independent high-pressure Roots
blower with a power of 1.5-2.0 kW/100m 3 . The daytime light intensity in the aquaculture
plant is 500-5000 Lux. The water in the culture pond first flows through a microfilter to
remove solid particles including residual bait and feces, then it is pumped by a water
circulating pump to enter a biofilter, and then flows back to the culture pond after being
sterilized by an ultraviolet sterilization device.
The recirculating aquaculture method is composed of the following steps. Using sand
filtered seawater as aquaculture water, which is treated by bleaching powder in a
reservoir, fully aerated to remove residual chlorine and then pumped into the aquaculture
system. The working frequency of the water-circulating pump is adjusted to make the
water circulation amount of the culture pond be 2-3 times/day. Feeding 5-6 times a day
and discharging sewage before feeding. After the system runs stably for 10~15 days, 40
% brown granulated sugar or 50-60% molasses is added to the culture pond every day
to promote the growth of heterotrophic bacteria. The total number of bacteria in the
culture pond is 1.3~4.7 x 107 CFU/mL, and the proportion of Rhodobacteraceaerich in
probiotics increases to more than 50%, of Flavobacteriaceaebacteria in the bacterial community decreases to less than 20%, of Planctomycetaceae increases to more than
%, and the proportion of nitrifying bacteria maintains at 1.00-2.00%.
The system is stable during operation with good water quality, with ammonia
concentration of 0.21-1.03 mg/L and average concentration of 0.56 mg/L. The
concentration of nitrite ranges from 0.04 to 1.21 mg/L, with an average concentration of
0.51 mg/L, and the average daily water exchange volume is less than 5%.
Compared with the prior art, the invention has the following advantages.
(1) The present invention combines nitrification and assimilation of bacteria to treat
water of recirculating aquaculture system. With nitrification as the core of water
treatment, carbon sources are continuously increased during the cultivation, so that while
promoting the growth of Rhodobacteraceae bacteria in the aquaculture waters and
enhancing the assimilation of bacteria, the nitrification of nitrifying bacteria is not
inhibited. The assimilation and nitrification together purify the water body, thereby
improving the microbial water treatment efficiency, reducing the load of the water
treatment system and improving the aquaculture environment.
(2) Using the assimilation of bacterial to purify water reduces the load of water
treatment system, thus reducing the water circulation rate and the volume of biofilter,
achieving the effects of reducing energy consumption, changing water and volume of
biofilter, improving the utilization rate of resources and improving the production
efficiency.
(3) The relatively turbid water under low water circulation rate is more suitable for the
cultivation of Penaeus vannamei, which reduces the stimulation of excessive light on the
prawns during the day.
(4) Adding carbon source not only can enhance the assimilation of bacteria, but also can
regulate the bacterial colony structure of the system, consolidate the dominant position of
Rhodobacteraceaebacteria rich in probiotics in water colonies. In addition, it can inhibit
some potential pathogens of Flavobacteriaceae, and promote the growth of
Planctomycetaceaebacteria rich in nitrogen cycle-related bacteria in biofilm.
Figure 1 is a schematic structural diagram of the recirculating aquaculture method of
Penaeusvannamei.
Figure 2 is the aeration arrangement for the culture pond in the present invention.
In figures, 1- culture pond, 2- water inlet direction, 3- rotation direction of water body in
culture pond, 4- nano-air pipe.
The application of the invention in the recirculating aquaculture of Penaeus vannamei
will be explained in detail with application embodiments.
Embodiment 1
As shown in Fig. 1, in this aquaculture system, there were 6 culture ponds with a
specification of 6.5 x 6.5 m and a water depth of 0.9 m. The culture pond had a conical bottom with a slope of 1/15-1/20, and the central blowdown method was adopted with the blowdown pipe shared with the circulating backwater pipe.
Belt microfilter with screen size of 120 meshes was used, which can backwash
automatically according to liquid level difference. The water flow rate of the water
circulating pump is 25-30 m 3/h, and the water in the culture pond circulated 2-3 times a
day. The size of biofilter was 7.45 x 3.0 x 2.6 m, which was full of brush medium with a
filling rate of 50%. Before starting the system, the biofilter was cultured in advance to
construct the nitrification system. The rated power of the ultraviolet sterilization device
was 2.4 kw, and the power used was divided into four grades. Roots blower for aeration
had a power of 3 kw, and nano-air pipes were arranged around the culture pond and fixed
at the bottom to push the water to rotate while inflating, which is convenient for
collecting pollution, as shown in Fig. 2. The practical power of aeration blower in
biofilter was 1.5 kw, and aeration disk was used for aeration. The top part of the plant
was partially transparent, and the daytime light intensity was 500-5000 Lux. The inner
wall of the workshop was equipped with thermal insulation materials to reduce heat loss.
After sand filtration, the offshore seawater was pumped into the reservoir, treated with
% bleaching powder, fully aerated to remove residual chlorine, heated by a heat pump
and pumped into the circulating water culture system. The length of Penaeus vannamei
seeds was 3-4 cm, the stocking density was 400/m2 , and the circulating water system was
started 2-3 days after the shrimp were released. Feeding 5 times a day and discharging
sewage manually before feeding. Regularly monitoring the number of Vibrio in the
system and starting sterilization when the number of Vibrio exceeds 8000 CFU/mL. After
the system ran stably for 15 days, brown granulated sugar was added into the system according to the mass ratio of 40% of the daily feeding amount to assist in water purification and regulate the microbial community in the system. Regularly monitoring the water quality indicators of the system to guide the system management.
During the culture period, the system ran well. Wherein, the concentrations of ammonia
and nitrite were less than 0.5 mg/L, DO was greater than 5.2 mg/L, temperature was
27.2-29.1 °C, salinity was 29.1-30.2, and pH was 7.7-7.9. The average daily water
exchange rate was less than 5%, which greatly reduced the loss of water and energy. The
number of Vibrio was less than 1.2 x 104 CFU/mL, with an average of 0.67 x 104
CFU/mL, accounting for 0.1 6 ~0. 5 7 % in the bacterial community. Rhodobacteraceae
family occupied an absolute advantage in the bacterial community of aquaculture water,
accounting for 56.93~60.06%. The proportion of Flavobacteriaceaebacteria in water
colonies decreased to 17.59~19.21%. In the biofilm, the proportion of bacteria belonging
to the family Planctomycetaceaein the colony reached 23.17~49.22%, and the proportion
of nitrifying bacteria in the colony was 1.01~3.76%. The growth of Penaeus vannamei
was in good condition. After 65 days, the load of prawn aquaculture is 4.2 kg/m2 , and the
water consumption was about 1.07 m 3/kg prawn, indicating good economic benefits.
Embodiment 2
The culture system and management were the same as those in Embodiment 1, while no
carbon source was added during the culture process, and the water quality was controlled
by nitrification of biofilter and physical filtration. In the early stage of culture, the system
ran well, and the concentrations of ammonia and nitrite were controlled below 0.5 mg/L.
However, with the growth of prawn, the feeding amount of bait increased greatly, the water treatment load of the system increased, and the concentration of ammonia and nitrite gradually increased in the late stage. Wherein, the concentration of ammonia and nitrite reached 3.1 mg/L and 5.4 mg/L in the late stage of culture respectively, which threatened the healthy culture of prawn. After 65 days, the prawn culture load was 2.8 kg/m2 .
Embodiment 3
The culture system and management were the same as those in Embodiment 1, which no
carbon source was added during the culture process. The culture water environment was
regulated by increasing the water circulation rate of the system and strengthening
nitrification in the biofilter. The water circulation rate of the system was 10-15 times/day.
The water quality indexes such as ammonia and nitrite were well controlled and the
culture water was clear, but it was not suitable for Penaeus vannamei culture. The
diversity of colonies in aquaculture water was low, with Pseudoalteromonadaceae
accounting for 32.63%, Colwelliaceae accounting for 26.24% and Alteromonadaceae
accounting for 14.12%. However, Rhodobacteraceaewith more probiotics, did not form
a dominant position, accounting for only 15.63% of the total bacteria. The colony
structure in aquaculture water was unfavorable to the growth of prawns, and the mortality
of prawns was high, reaching 60%. After 65 days, the prawn culture load was only 1.1
kg/m2 .
Embodiment 4
The culture system and management were the same as those in Embodiment 1. The
addition of carbon source was increased to enhance bacterial assimilation, and the addition of brown granulated sugar was 70% of the bait feeding amount. The water quality control effect was good, wherein, ammonia and nitrite were controlled within 0.3 mg/L. The high carbon-nitrogen ratio did promote the large-scale reproduction of heterotrophic bacteria, but it also leaded to excessive residues in the system pipeline and sedimentation in the biofilter, which increased the cost of dredging the pipeline and biofilter. On one hand, the large addition of brown granulated sugar would also increase the cost. On the other hand, nitrifying bacteria in biofilter were greatly inhibited under the influence of high carbon-nitrogen ratio, accounting for less than 0.1% of the total bacteria, which weakened the function of biofilter. After 65 days, the loading capacity of prawn culture was similar to that of Embodiment 1, about 4.0 kg/m2 , while the input cost of high carbon-nitrogen ratio was high, which could not improve the culture income.
Claims (3)
1. A prawn recirculating aquaculture method combined with nitrification and
assimilation, characterized in that its aquaculture system specifically comprises multiple
culture ponds, a microfilter, a water-circulating pump, a biofilter, an ultraviolet
sterilization device and a Roots blower. Wherein, the culture pond has a conical bottom
with a slope of 1/15~1/20, and the central blowdown method is adopted with the
blowdown pipe shared with the circulating backwater pipe. Nano-air pipes are arranged
around the culture pond and fixed at the bottom of the pond to push the water to rotate
while inflating, which is convenient for collecting pollution. The microfilter is belt type
or drum type, and the screen specification is 100-120 meshes. The water-circulating
pump is frequency conversion type, which is convenient to control the flow rate. The
volume of the biofilter is 1/5-1/4 of that of the aquaculture waters, and the biofilter is
filled with brush medium or porous suspended medium, wherein, the filling rate of brush
medium is 40-50% and porous suspended medium is 20~30%. The power of the
ultraviolet germicidal lamp is 0.5-1.0 kw/100m 3. It is not necessary to start ultraviolet
sterilization in the early stage of culture to promote the biofilm formation. When the
number of Vibrio exceeds 8000 CFU/mL, the ultraviolet germicidal lamp is started for
sterilization. The power of Roots blower is 0.7~1.0 kW/100m
2 . The biofilter is inflated by
an independent high-pressure Roots blower with a power of 1.5-2.0 kW/100m 3 . The
daytime light intensity in the aquaculture plant is 500-5000 Lux. The water in the culture
pond first flows through a microfilter to remove solid particles including residual bait and
feces, then it is pumped by a water-circulating pump to enter a biofilter, and then flows
back to the culture pond after being sterilized by an ultraviolet sterilization device.
The recirculating aquaculture method is composed of the following steps. Using sand
filtered seawater as aquaculture water, which is treated by bleaching powder in a
reservoir, fully aerated to remove residual chlorine and then pumped into the aquaculture
system. The working frequency of the water-circulating pump is adjusted to make the
water circulation amount of the culture pond be 2-3 times/day. Feeding 5-6 times a day
and discharging sewage before feeding. After the system runs stably for 10~15 days, 40
% brown granulated sugar or 50-60% molasses is added to the culture pond every day
to promote the growth of heterotrophic bacteria. The total number of bacteria in the
culture pond is 1.
3~4.7 x 107 CFU/mL, and the proportion of Rhodobacteraceaerich in
probiotics increases to more than 50%, of Flavobacteriaceaein the bacterial community
decreases to less than 20%, of Planctomycetaceae increases to more than 20%, and the
proportion of nitrifying bacteria maintains at 1. 0 0 -2 . 0 0 %.
The system is stable during operation with good water quality, with ammonia
concentration of 0.21-1.03 mg/L and average concentration of 0.56 mg/L. The
concentration of nitrite ranges from 0.04 to 1.21 mg/L, with an average concentration of
0.51 mg/L, and the average daily water exchange volume is less than 5%.
-1/2- 2021100324
Figure 1
The schematic structural diagram of the recirculating aquaculture method of Penaeus
vannamei.
-2/2- 2021100324
Figure 2
The aeration arrangement for the culture pond in the present invention.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2021100324A AU2021100324A4 (en) | 2021-01-19 | 2021-01-19 | A Prawn Recirculating Aquaculture Method Combined with Nitrification and Assimilation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2021100324A AU2021100324A4 (en) | 2021-01-19 | 2021-01-19 | A Prawn Recirculating Aquaculture Method Combined with Nitrification and Assimilation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2021100324A4 true AU2021100324A4 (en) | 2021-04-15 |
Family
ID=75397062
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2021100324A Active AU2021100324A4 (en) | 2021-01-19 | 2021-01-19 | A Prawn Recirculating Aquaculture Method Combined with Nitrification and Assimilation |
Country Status (1)
| Country | Link |
|---|---|
| AU (1) | AU2021100324A4 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113875680A (en) * | 2021-11-03 | 2022-01-04 | 广东海洋大学 | Simple economic experimental circulating water culture system and culture process thereof |
-
2021
- 2021-01-19 AU AU2021100324A patent/AU2021100324A4/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113875680A (en) * | 2021-11-03 | 2022-01-04 | 广东海洋大学 | Simple economic experimental circulating water culture system and culture process thereof |
| CN113875680B (en) * | 2021-11-03 | 2023-12-15 | 广东海洋大学 | Simple economic experiment type circulating water culture system and culture process thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7001519B2 (en) | Integrated closed loop system for industrial water purification | |
| CN108432685B (en) | Water environment regulation and control and recirculating aquaculture system for high-load aquaculture | |
| CN101407360B (en) | Method for processing waste water of circulating marine culture by using artificial wet land | |
| CN105638527B (en) | A kind of culture of Penaeus vannamei method of green high yield | |
| CN105417856A (en) | Closed-recirculation multistage biological purification system | |
| CN1981574A (en) | Aquiculturing system process for works with circulating water | |
| CN108094301B (en) | Circulating water culture system based on biological flocculation ectopic treatment and use method | |
| CN101327997A (en) | Comprehensive processing method of aquiculture sewerage | |
| CN104150684A (en) | Aquaculture wastewater treatment process | |
| CN110521648A (en) | A kind of joint nitrification-assimilation prawn circulating water cultivation method | |
| CN106035190A (en) | Dual-channel water processing method of factory-like circulating sea water system | |
| CN113636727A (en) | Land-based circular pond circulating culture tail water treatment system and method | |
| CN109942156A (en) | Sea-farming tail water treatment system and its application method based on biofilter | |
| AU2021100324A4 (en) | A Prawn Recirculating Aquaculture Method Combined with Nitrification and Assimilation | |
| CN109937924B (en) | Breeding method for improving quality of euryhaline fishes in low-salinity area | |
| JP2020074761A (en) | Breeding water quality management system for circulation type on-land culture and operation method | |
| CN201365459Y (en) | Fish-algae industrial cultivation combined circulation system | |
| CN108164086B (en) | Breeding wastewater recycling system and application thereof in recycling breeding wastewater | |
| CN105060648A (en) | Industrial circulating water fish-culture denitrification zero-emission system | |
| CN218642562U (en) | Water ecological treatment system | |
| CN109912134A (en) | A kind of freshwater aquiculture tail water treatment system | |
| CN107265755B (en) | Closed circulation aquaculture water treatment system and method | |
| CN108633802A (en) | A kind of method of biological breeding Penaeus Vannmei parent shrimp | |
| CN203206967U (en) | Circulating water cultivation system based on microecological balance regulation | |
| CN106719159A (en) | A kind of Anguilla marmorata feeds mode |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGI | Letters patent sealed or granted (innovation patent) |