AU2020103164A4 - A Method to Enhance the Drought Tolerance of Potato Plants - Google Patents
A Method to Enhance the Drought Tolerance of Potato Plants Download PDFInfo
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- 244000061456 Solanum tuberosum Species 0.000 title claims abstract description 93
- 238000000034 method Methods 0.000 title claims abstract description 40
- 230000024346 drought recovery Effects 0.000 title claims abstract description 29
- 238000002791 soaking Methods 0.000 claims abstract description 33
- 230000002708 enhancing effect Effects 0.000 claims abstract description 15
- 238000005507 spraying Methods 0.000 claims abstract description 10
- 238000002360 preparation method Methods 0.000 claims abstract description 8
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 45
- IXVMHGVQKLDRKH-QHBHMFGVSA-N 24-epi-brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-QHBHMFGVSA-N 0.000 claims description 24
- UDPGUMQDCGORJQ-UHFFFAOYSA-N (2-chloroethyl)phosphonic acid Chemical compound OP(O)(=O)CCCl UDPGUMQDCGORJQ-UHFFFAOYSA-N 0.000 claims description 21
- 239000005976 Ethephon Substances 0.000 claims description 20
- 238000009210 therapy by ultrasound Methods 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 6
- 238000005057 refrigeration Methods 0.000 claims description 3
- 230000008641 drought stress Effects 0.000 abstract description 29
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000001766 physiological effect Effects 0.000 abstract description 3
- 231100000331 toxic Toxicity 0.000 abstract description 3
- 230000002588 toxic effect Effects 0.000 abstract description 3
- 230000008635 plant growth Effects 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 20
- 230000000694 effects Effects 0.000 description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 19
- JLIDBLDQVAYHNE-YKALOCIXSA-N Abscisic acid Natural products OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 18
- 102000004190 Enzymes Human genes 0.000 description 16
- 108090000790 Enzymes Proteins 0.000 description 16
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 9
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 9
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 8
- 230000000243 photosynthetic effect Effects 0.000 description 8
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 description 6
- IXVMHGVQKLDRKH-KNBKMWSGSA-N brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-KNBKMWSGSA-N 0.000 description 6
- 238000009825 accumulation Methods 0.000 description 5
- 230000003247 decreasing effect Effects 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 230000002000 scavenging effect Effects 0.000 description 5
- 239000003963 antioxidant agent Substances 0.000 description 4
- 230000003078 antioxidant effect Effects 0.000 description 4
- 239000003642 reactive oxygen metabolite Substances 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 230000035882 stress Effects 0.000 description 4
- 239000003337 fertilizer Substances 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 230000029553 photosynthesis Effects 0.000 description 3
- 238000010672 photosynthesis Methods 0.000 description 3
- 239000003375 plant hormone Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
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- 230000003111 delayed effect Effects 0.000 description 2
- 230000004792 oxidative damage Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000554155 Andes Species 0.000 description 1
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- 206010021143 Hypoxia Diseases 0.000 description 1
- 241000208292 Solanaceae Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 208000035199 Tetraploidy Diseases 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- HLVPIMVSSMJFPS-UHFFFAOYSA-N abscisic acid beta-D-glucopyranosyl ester Natural products O1C(CO)C(O)C(O)C(O)C1OC(=O)C=C(C)C=CC1(O)C(C)=CC(=O)CC1(C)C HLVPIMVSSMJFPS-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
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- 229930002877 anthocyanin Natural products 0.000 description 1
- 235000010208 anthocyanin Nutrition 0.000 description 1
- 239000004410 anthocyanin Substances 0.000 description 1
- 150000004636 anthocyanins Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
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- 210000002390 cell membrane structure Anatomy 0.000 description 1
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- 230000035558 fertility Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 230000008119 pollen development Effects 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
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- 230000005070 ripening Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000008117 seed development Effects 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
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- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
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- 229940088594 vitamin Drugs 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
- A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for enhancing the drought tolerance of potato plants,
which comprises the following steps: Sl. Preparation of soaking solution; S2.
Pretreatment; S3. Spraying. The method of the invention can effectively improve the
drought tolerance of potato plants, significantly alleviate the wilting state of potato plants
under drought stress, and maintain plant growth and normal physiological activities. The
invention has the advantages of simple operation, easy popularization, no toxic side-effect,
low cost, no pollution to the environment and suitable for large-scale popularization and
use.
1/4
90
85
-80
0T
0t
~75
E
~70
65
60
0 0.125 0.25 0.5 1 2
Blank group and embodiments 1-5
Figure 1
95
~OWN
o 5
95
-4- Contrast group 1
- Contrast group 2 ~:80
- Blank group
75--M-
Inventive group
70
-3 0 3 6 9
Days of drought stress (days)
Figure 2
Description
1/4
90
85
-80
0T 0t ~75
~70
65
60 0 0.125 0.25 0.5 1 2 Blank group and embodiments 1-5
Figure 1
95
~OWN 95 o 5
-4- Contrast group 1
- Contrast group 2 ~:80 - Blank group Inventive group 75--M-
70 -3 0 3 6 9 Days of drought stress (days)
Figure 2
A Method to Enhance the Drought Tolerance of Potato Plants
[01] The invention belongs to the technical field of potato cultivation, in particular to a method for enhancing the drought tolerance of potato plants.
[02] Potato is an annual herbaceous tuber of Solanaceae, which originated from the plateau area of Andes Mountains in Latin America. After being domesticated, it gradually became a large-scale cultivated tetraploid plant and is the fourth largest food crop in the world. In addition to being rich in starch, potatoes contain many health promoting nutrients, such as phenols, anthocyanins, carotenoids, flavonoids, and many vitamins. China is a big potato producing country, and its output is more than a quarter of the world's, but most of the major potato production areas in China are located in arid, semi-arid areas. With the global warming and continuous deterioration of climate environment, water shortage problem has becoming increasingly prominent. Drought has gradually become an important limiting factor for potato growth and development. Potato is a typical temperate climate crop, and drought greatly affects yield and quality of potato. At present, the main researches on enhancing drought tolerance of potato are as follows: identification and screening of drought-resistant germplasm resources, screening of drought-resistant genes and acquisition of transformed lines through different fertilizer ratio and fertilizer application. However, long-term application of chemical fertilizer may cause environmental pollution and harm to the structure and physical and chemical properties of soil. The screening of germplasm resources is heavy workload and time-consuming. The screening and transformation of drought tolerant genes are characterized by long time-consuming, low mutation efficiency and high cost. Therefore, it is necessary to provide an effective, convenient and affordable measure for potato to resist drought stress, which is necessary to improve the yield and quality of potato under drought stress.
[03] Ethephon, scientific name: 2-chloroethyl phosphonic acid, molecular formula: C2H6CIO3P, molecular weight 144.49, is a low toxic plant growth regulator, releasing plant hormone ethephon, widely used in fruits and vegetables for ripening, but also used in flowers to induce flower bud formation and flowering, but ethephon is rarely used in the field of plant tolerance research and application. The reason is that through the study of the relationship between ethephon and plant tolerance, the domestic researchers have found inconsistent results, that is, ethephon is both promote and inhibit the drought tolerance of plants, but at present the domestic and foreign research has not clearly revealed the mechanism of the action, therefore, the application of ethephon in the promotion of drought tolerance is limited to a certain extent. Different plants have different sensitivity to ethephon, which determines the physiological and molecular response of different crops to ethephon. The effect of ethephon on drought tolerance of plants is related to many factors, such as plant species, varieties, solution concentration, treatment method, time of drought treatment and severity of drought.
[04] Brassinolide (BR), also known as brassinolide, is listed as the sixth type of plant hormone and distributed in various plant organs. Brassinolide can promote seed development and germination, promote plant cell division and elongation, promote vascular differentiation, improve photosynthesis, affect pollen development and fertility, and increase crop yield and quality. At the same time, BR can enhance plant tolerance to drought, hypoxia, high salt, weak light, bacteria and other adverse conditions. In recent years, 2, 4-epibrassinolide (2, 4-epigallocinolide, EBR) has been widely used as a plant hormone in plant tolerance to biological and abiotic stresses, and has been reported. brassinolide has good application prospect in plant adversity because of its high bioactivity, low price, no pollution to environment, no toxic side effect to plant.
[05] The purpose of the invention is to provide a method to enhance the drought tolerance of potato plants, which can provide an effective, convenient and affordable measure for potato to resist drought stress, improve the drought tolerance of potato plants, alleviate the wilting state of potato plants under drought stress, and maintain the growth and normal physiological activities of potato plants.
[06] The technical scheme adopted by the invention is as follows:
[07] A method to enhance the drought tolerance of potato plants includes the following steps:
[08] Si. Preparation of soaking solution: 2, 4-epibrassinolide solution with concentration of 0.1-24mol/L and ethephon solution with concentration of 80-100mg/L were mixed according to volume ratio of 1: 1, and then heated to 35-45 °C for ultrasonic treatment to obtain the soaking solution.
[09] S2. Pretreatment: The potato tubers with bud eyes were refrigerated at 2 °C, then the tubers were soaked in the soaking solution obtained by S Istep, and the pre-treated potato tubers were obtained.
[010] S3. Spraying: Cultivate the pre-treated potato tubers obtained in S2 step. In rosette stage, potato leaves were sprayed with 2, 4-epibrassinolide solution under dark condition continuously for 3 days until the leaves were dripping; the dripping state of leaves was just the beginning of dripping.
[011] Under drought stress, the unbalance of active oxygen generation and scavenging in potato leaves resulted in the increase of H202 and02- content. The enzyme activity of SOD, POD and CAT of antioxidant enzymes increased rapidly first, participated in the scavenging reactive oxygen species, and then decreased in the later stage. The EBR treatment delayed the occurrence of stress, the enzyme activity of SOD, POD and CAT began to increase in the late stage of drought stress, and the enzyme activity of SOD and POD increased greatly, and the ability of scavenging ROS was stronger, thus the accumulation of H202and02was decreased. Excessive accumulation of ROS under drought stress damaged the integrity of cell membrane structure, leading to the increase of MDA content, the leakage of electrolyte and the increase of relative conductivity. EBR treatment decreased MDA content and electrical conductivity, and maintained the stability of cell membrane.
[012] Under drought stress, the contents of soluble sugar, soluble protein, proline and ABA in potato leaves increased rapidly, the stability of cell membrane was maintained, and the content of soluble protein decreased in late stage of stress. EBR treatment delayed the occurrence of stress, increased the content of soluble sugar and proline slowly, alleviated the degradation of soluble protein in the late stage of stress, increased the content of ABA under drought stress, maintained the ability of cell osmotic regulation, and enhanced the drought tolerance.
[013] Furthermore, ultrasonic treatment is performed for 2-4h in step Si, preferably for 3 h.
[014] Furthermore, the refrigeration time in S2 step is 3-6h; preferably for 5h.
[015] Furthermore, the specific process of soaking in S2 step is: the frozen tubers are soaked in the soaking solution at 40 °C for 2-4 hours, then the soaking solution is naturally cooled to 25 °C and soaked for 24-36 hours.
[016] Furthermore, the concentration of 2, 4-epibrassinolide solution in the S3 step is 0.1-2mol/L.
[017] Furthermore, the concentration of 2, 4-epibrassinolide solution in the S3 step is 0.5 [mol/L.
[018] To sum up, due to the adoption of the above-mentioned technical scheme, the beneficial effect of the invention is:
[019] 1. By the method of the invention, the higher leaf water content of the potato plant under drought stress is maintained, the photosynthesis of the plant is maintained, and the higher net photosynthetic rate is guaranteed.
[020] 2. The invention enhances the activity of SOD and POD antioxidant enzymes, improves the ability of scavenging reactive oxygen species, reduces the accumulation of H202, and reduces the oxidative damage to potato plants;
[021] 3. The invention also delays the increase of proline content and enhances the tolerance of potato plants to drought stress.
[022] 4. The invention increases the content of abscisic acid in potato plants and enhances the drought tolerance of potato plants;
[023] 5. The invention effectively improves the drought tolerance of potato, significantly alleviates the wilting state of potato plants under drought stress, maintains the growth and normal physiological activities of the plants, and has the advantages of simple operation, easy popularization, non-toxic side effect, no pollution to the environment, low cost and suitable for large-scale popularization and use.
[024] In order to more clearly explain the technical scheme of the embodiment of the invention, the appended drawings to be used in the embodiment are briefly introduced below. It should be understood that the appended drawings below show only some embodiments of the invention, and therefore should not be regarded as a limitation of scope. For ordinary technicians in this field, other relevant appended drawings can be obtained without paying creative labor.
[025] Figure 1 Water content of potato plants leaves on the 9th day of drought stress in blank group and embodiments 1-5.
[026] Figure 2 Changes of water content of potato leaves during 9 days of drought stress treated by the method;
[027] Figure 3 Changes of net photosynthetic rate of potato leaves during 9 days of drought stress treated by the method;
[028] Figure 4 Changes of SOD activity of potato leaves during 9 days of drought stress treated by the method.
[029] Figure 5 Change of POD enzyme activity of potato leaves after 9 days of drought stress treated by the method.
[030] Figure 6 Changes of H202 content of potato leaves after 9 days of drought stress treated by the method;
[031] Figure 7 Change of abscisic acid content of potato leaves after 9 days of drought stress treated by the method.
[032] Figure 8 Changes of proline content of potato leaves after 9 days of drought stress treated by the method.
[033] In order to make the purpose, technical scheme and advantages of the invention clearer, a further detailed description of the invention is given in combination with the appended drawings and embodiments. It should be understood that the specific embodiments described here are used only to explain the invention and are not used to limit the invention, that is, the embodiments described are only partial embodiments of the invention, not all of them. Components of the embodiment of the invention usually described and shown in the appended drawings here can be arranged and designed in a variety of different configurations.
[034] Therefore, the following detailed description of the embodiment of the invention provided in the appended drawings is not intended to limit the scope of the invention for which protection is claimed, but merely represents the selected embodiment of the invention. Based on the embodiment of the invention, all other embodiments obtained by the technical personnel in the field without making creative labor are within the scope of protection of the invention.
[035] It is important to note that relational terms such as "first" and "second" are used only to distinguish one entity or operation from another without necessarily requiring or implying that there is any such actual relationship or order between those entities or operations. Moreover, the term "include", "contain", or any other variation thereof is intended to cover non-exclusive inclusion, so that the process, method, article, or device including a series of elements includes not only those elements but also other elements not specifically listed, or elements inherent in such process, method, article, or device. In the absence of more restrictions, the elements defined by the statement "including a..." are not excluded from the existence of the same elements in the process, method, article or device that includes the elements.
[036] A further description of the characteristics and performance of the invention is given in combination with an embodiment below.
[037] Embodiment 1
[038] The invention provides a method for enhancing the drought tolerance of potato plants, which comprises the following steps:
[039] Si. Preparation of soaking solution: 2, 4-epibrassinolide with concentration of 0.125 mol/L and ethephon solution with concentration of 80 mg/L were mixed
according to volume ratio 1: 1, heated to 40 °C and ultrasonic treatment for 3 hours
while hot to obtain the soaking solution.
[040] S2. Pretreatment: The potato tubers with bud eyes were refrigerated at 3 °C for 4 hours, then the frozen tubers were soaked in soaking solution at 40 °C for 3 hours,
then the soaking solution was naturally cooled to 25 °C and soaked for 24 hours.
[041] S3. Spraying: Cultivate the pre-treated potato tubers obtained in S2 step. In rosette stage, potato leaves were sprayed with 2, 4-epibrassinolide solution of 0.125
[mol/L under dark condition continuously for 3 days until the leaves began to drip.
[042] Embodiment 2
[043] The invention provides a method for enhancing the drought tolerance of potato plants, which comprises the following steps:
[044] S1. Preparation of soaking solution: 2, 4-epibrassinolide with concentration of 0.25 mol/L and ethephon solution with concentration of 80 mg/L were mixed
according to volume ratio 1: 1, heated to 40 °C and ultrasonic treatment for 3 h while
hot to obtain the soaking solution.
[045] S2. Pretreatment: The potato tubers with bud eyes were refrigerated at 3 °C for 4 hours, then the frozen tubers were soaked in soaking solution at 40 °C for 3 hours, then the soaking solution was naturally cooled to 25 °C and soaked for 24 hours.
[046] S3. Spraying: Cultivate the pre-treated potato tubers obtained in S2 step. In rosette stage, potato leaves were sprayed with 2, 4-epibrassinolide solution of 0.25
[mol/L under dark condition continuously for 3 days until the leaves began to drip.
[047] Embodiment 3
[048] The invention provides a method for enhancing the drought tolerance of potato plants, which comprises the following steps:
[049] Si. Preparation of soaking solution: 2, 4-epibrassinolide with concentration of 0.5 mol/L and ethephon solution with concentration of 80 mg/L were mixed
according to volume ratio 1: 1, heated to 40 °C and ultrasonic treatment for 3 h while
hot to obtain the soaking solution.
[050] S2. Pretreatment: The potato tubers with bud eyes were refrigerated at 3 °C for 4 hours, then the frozen tubers were soaked in soaking solution at 40 °C for 3 hours, then the soaking solution was naturally cooled to 25 °C and soaked for 24 hours.
[051] S3. Spraying: Cultivate the pre-treated potato tubers obtained in S2 step. In rosette stage, potato leaves were sprayed with 2, 4-epibrassinolide solution of 0.5
[mol/L under dark condition continuously for 3 days until the leaves began to drip.
[052] Embodiment 4
[053] The invention provides a method for enhancing the drought tolerance of potato plants, which comprises the following steps:
[054] S1. Preparation of soaking solution: 2, 4-epibrassinolide with concentration of 1 mol/L and ethephon solution with concentration of 80 mg/L were mixed
according to volume ratio 1: 1, heated to 40 °C and ultrasonic treatment for 3 hours
while hot to obtain the soaking solution;
[055] S2. Pretreatment: The potato tubers with bud eyes were refrigerated at 3 °C for 4 hours, then the frozen tubers were soaked in soaking solution at 40 °C for 3 hours,
then the soaking solution was naturally cooled to 25 °C and soaked for 24 hours.
[056] S3. Spraying: Cultivate the pre-treated potato tubers obtained in S2 step. In rosette stage, potato leaves were sprayed with 2, 4-epibrassinolide solution of 1 mol/L under dark condition continuously for 3 days until the leaves began to drip.
[057] Embodiment 5
[058] The invention provides a method for enhancing the drought tolerance of potato plants, which comprises the following steps:
[059] Si. Preparation of soaking solution: 2, 4-epibrassinolide with concentration of 2 mol/L and ethephon solution with concentration of 80 mg/L were mixed
according to volume ratio 1: 1, heated to 40 °C and ultrasonic treatment for 3 hours
while hot to obtain the soaking solution;
[060] S2. Pretreatment: The potato tubers with bud eyes were refrigerated at 3 °C
for 4 hours, then the frozen tubers were soaked in soaking solution at 40 °C for 3 hours,
then the soaking solution was naturally cooled to 25 °C and soaked for 24 hours.
[061] S3. Spraying: Cultivate the pre-treated potato tubers obtained in S2 step. In rosette stage, potato leaves were sprayed with 2, 4-epibrassinolide solution of 2 mol/L under dark condition continuously for 3 days until the leaves began to drip.
[062] Experimental Example 1
[063] Set up the blank control group not soaked in 2, 4-epibrassinolide solution and ethephon solution. The water content of soil in the cultivation nutrition bowl of the blank group and the embodiments 1-5 were similar and about 70% of the maximum water-holding capacity. After spraying water to the blank group and spraying to embodiments 1-5, the water content of leaves was measured after natural water cut-off and drought treatment for 9 days. The results were as follows:
[064] As shown in Figure 1, the leaf water content of drought potato plants in blank group (coordinate 0) was 73.06%, the leaf water content of potato plants in embodiment group 1(coordinate 0.125) was 76.37%, the leaf water content of potato plants in embodiment group 2(coordinate 0.25) was 76.38%, and the leaf water content of potato plants in embodiment group 3(coordinate 0.5) was 84.76%. The leaf water content of potato plants in 4 groups (horizontal coordinates 1) was 78.85%, and that of potato plants in 5 groups (horizontal coordinates 2) was 79.03%.
[065] Experimental Example 2
[066] The potato plants which had not been soaked with 2, 4-epibrassinolide solution and ethephon solution and that had not been sprayed with 2, 4-epibrassinolide solution were treated with natural drought as blank group. The plant obtained under the treatment condition of embodiment 3 to undergo drought treatment was set up as the inventive group. The potato plants which are not soaked in 2, 4-epibrassinolide solution and ethephon solution and not sprayed with 2, 4-epibrassinolide solution and ethephon solution to undergo normal watering treatment were set as contrast group 1. The plant obtained under the treatment condition of embodiment 3 to undergo normal watering treatment was set up as the contrast group 2. The soil moisture content of the above groups was similar and about 70% of the maximum water holding capacity. During the process of natural drought or normal watering for 9 days, the physiological indexes such as leaf water content, net photosynthetic rate, SOD and POD antioxidant enzyme activity, H202 content and abscisic acid content were measured, and the accumulation of reactive oxygen species such as H202 and superoxide anion were recorded after 9 days of treatment. The results were as follows:
[067] (1) As shown in Figure 2-8, leaf water content, net photosynthetic rate, SOD enzyme activity, POD enzyme activity, H202 content, abscisic acid content and proline content of potato plants in contrast group 1 were 100%, 72.89%, 117.8%, 97.1%, 108.8%, 110.5%, 110. 3 % of those of 0 day drought stress treatment, respectively.
[068] (2) As shown in Figure 2-8, leaf water content, net photosynthetic rate, SOD enzyme activity, POD enzyme activity, H202 content, abscisic acid content and proline content of potato plants in contrast group 2 were 99.2%, 73.68%, 108.4%, 102.5%, 98.8%, 108.1%, 125.6% of those of 0 day drought stress treatment, respectively.
[069] (3) As shown in Figure 2-8, the leaf water content, net photosynthetic rate, SOD enzyme activity, POD enzyme activity, H202 content, abscisic acid content and proline content of potato plants in inventive group were 90.28%, 40.17%, 277.5%, 203.5%, 223.2%, 154.8%, 951.9% of those of 0 day drought stress treatment, respectively. The plant status and physiological indexes were close to the contrast group.
[070] (4) As shown in Figure 2-8, leaf water content, net photosynthetic rate, SOD enzyme activity, POD enzyme activity, H202 content, abscisic acid content and proline content of potato plants in blank group were 79.32%, 6.50%, 200.3%, 164.6%, 260.0%, 132.9%, 1499.6% of those of 0 day drought stress treatment, respectively. The plant status and physiological indexes were significantly lower than those of the inventive group.
[071] To sum up, through the method of the invention, the higher leaf water content of potato plants under drought stress is maintained, the photosynthesis of plants is maintained, and the higher net photosynthetic rate is guaranteed. At the same time, the activity of SOD and POD antioxidant enzymes is enhanced, the scavenging ability of reactive oxygen species is improved, the accumulation of H202 is decreased, and the oxidative damage to potato plants is alleviated. The invention also delays the increase of proline content and enhance the tolerance of potato plants to drought stress. It also increases the content of abscisic acid in potato plants and enhances the drought tolerance of potato plants.
[072] Although the invention has been described with reference to specific examples, it will be appreciated by those skilled in the art that the invention may be embodied in many other forms, in keeping with the broad principles and the spirit of the invention described herein.
[073] The present invention and the described embodiments specifically include the best method known to the applicant of performing the invention. The present invention and the described preferred embodiments specifically include at least one feature that is industrially applicable
Claims (8)
1. A method to enhance the drought tolerance of potato plants, characterized by the following steps:
Si. Preparation of soaking solution: 2, 4-epibrassinolide solution with concentration of 0.1-2mol/L and ethephon solution with concentration of 80-100mg/L were mixed according to volume ratio of 1: 1, and then heated to 35-45 °C for ultrasonic treatment to obtain the soaking solution.
S2. Pretreatment: The potato tubers with bud eyes were refrigerated at 2-5 °C, then the tubers were soaked in the soaking solution obtained by S Istep, and the pre treated potato tubers were obtained.
S3. Spraying: Cultivate the pre-treated potato tubers obtained in S2 step. In rosette stage, potato leaves were sprayed with 2, 4-epibrassinolide solution under dark condition continuously for 3 days until the leaves were dripping.
2. The method for enhancing the drought tolerance of potato plants according to claim 1 is characterized by ultrasonic treatment for 2-4 hours in the Si step.
3. The method for enhancing the drought tolerance of potato plants according to claim 2 is characterized by ultrasonic treatment for 3 hours in the Si step.
4. The method for enhancing the drought tolerance of potato plants according to claim 1 is characterized by a refrigeration time of 3-6 hours in the S2 step.
5. The method for enhancing the drought tolerance of potato plants according to claim 4 is characterized by a refrigeration time of 5 hours in the S2 step.
6. The method for enhancing the drought tolerance of potato plants according to claim 1 is characterized in that the specific process of soaking in the S2 step is: the frozen tubers are soaked in the soaking solution at 40 °C for 2-4 hours, then the soaking solution is naturally cooled to 25 °C and soaked for 24-36 hours.
7. The method for enhancing the drought tolerance of potato plants according to claim 1 is characterized in that the concentration of 2, 4-epibrassinolide solution in the S3 step is 0.1-2mol/L.
8. The method for enhancing the drought tolerance of potato plants according to claim 1 is characterized in that the concentration of 2, 4-epibrassinolide solution in the S3 step is 0.5 [mol/L.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112514757A (en) * | 2021-01-26 | 2021-03-19 | 淮北师范大学 | Method for improving drought resistance of tobacco |
| CN114711106A (en) * | 2022-03-16 | 2022-07-08 | 河北省农林科学院旱作农业研究所 | Drought-resistant water-saving identification method for crop varieties |
| CN116616138A (en) * | 2023-07-13 | 2023-08-22 | 四川省中医药科学院 | Screening method of drought-resistant germplasm of ophiopogon japonicus |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112514757A (en) * | 2021-01-26 | 2021-03-19 | 淮北师范大学 | Method for improving drought resistance of tobacco |
| CN112514757B (en) * | 2021-01-26 | 2023-08-22 | 淮北师范大学 | Method for improving drought resistance of tobacco |
| CN114711106A (en) * | 2022-03-16 | 2022-07-08 | 河北省农林科学院旱作农业研究所 | Drought-resistant water-saving identification method for crop varieties |
| CN116616138A (en) * | 2023-07-13 | 2023-08-22 | 四川省中医药科学院 | Screening method of drought-resistant germplasm of ophiopogon japonicus |
| CN116616138B (en) * | 2023-07-13 | 2024-02-09 | 四川省中医药科学院 | Screening method of drought-resistant germplasm of ophiopogon japonicus |
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