AU2020101953A4 - A method of cultivating microalgae with high oil content - Google Patents
A method of cultivating microalgae with high oil content Download PDFInfo
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- AU2020101953A4 AU2020101953A4 AU2020101953A AU2020101953A AU2020101953A4 AU 2020101953 A4 AU2020101953 A4 AU 2020101953A4 AU 2020101953 A AU2020101953 A AU 2020101953A AU 2020101953 A AU2020101953 A AU 2020101953A AU 2020101953 A4 AU2020101953 A4 AU 2020101953A4
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
Here displays a method of cultivating microalgae with high lipid content, which was based
on the following steps: Firstly, microalgae (isochrysis galbana) were placed in a uniform
electric field with electric intensity of 1x106-2x106 V/m for 10-20 min, and then were placed
in a uniform magnetic field with magnetic intensity of 50-100 mT for 10-20min; Secondly, the
above microalgae (isochrysis galbana) were cultured according to the following parameters of
4-6 g/L of glucose as carbon source, seawater with salinity of 33-34 and pH of8.2-8.4, culturing
temperature with 19-26°C, light intensity with 3000-3500 lux, continuous aeration during
culture for 10-14 days. The microalgae(isochrysis galbana) with high lipid content was
obtained. This is a method of cultivating microalgae of high lipid content with low cost and
easy operation.
Description
A method of cultivating microalgae with high oil content
[01] The invention discloses a method to increase the lipid content of microalgae through uniform electric field and uniform magnetic field.
[02] Microalgae is a kind of autotrophic organism with high photosynthetic efficiency, which are widely lived in seawater and fresh water. Microalgae cells can produce polysaccharides, proteins, pigments, etc, hence it has a good business prospect in food, medicine, genetic engineering, liquid fuel, etc. Due to containing lipid, microalgae cells were thought as resources of refining bio-diesel. It has been extensively researched to refine microalgae biodiesel. Until now it is far from industrialized because of the high cost.
[03] Large scale cultivation of microalgae is a bottleneck technology on the industry of refining microalgae biodiesel. It is significant to achieve rapid growth of microalgae cell density and the increase of lipid content of microalgae cells.
[04] The invention provides a method of cultivating microalgae of high lipid content with low cost and easy operation.
[05] Technical solutions of the invention is how to culture microalgae of high lipid content, which are based on the following steps:
[06] Firstly, microalgae (isochrysis galbana) were placed in a uniform electric field with electric intensity of 1x106-2x106 V/m for 10-20 min, and then were placed in a uniform magnetic field with magnetic intensity of 50-100 mT for 10-20min.
[07] Secondly, the above microalgae (isochrysis galbana) were cultured according to the following parameters of 4-6 g/L of glucose as carbon source, seawater with salinity of 33-34 and pH of 8.2-8.4, culturing temperature with 19-26°C, light intensity with 3000-3500 lux, continuous aeration during culture for 10-14 days.
[08] The microalgae with high lipid content was obtained.
[09] The microalgae used in the invention are microalgae(isochrysisgalbana 8701) or microalgae (isochrysis galbana 3011).
[010] For culturing isochrysis galbana 8701 with high lipid content, microalgae cells was firstly in uniform electric field with electric intensity of1x106 V/m for 20min, and then was placed in uniform magnetic field with magnetic intensity of 100 mT for min; Secondly, the above microalgae (isochrysis galbana 8701) were cultured according to the following parameters of 4-6 g/L of glucose as carbon source, seawater with salinity of 33-34 and pH of 8.2-8.4, culturing temperature with 20±1°C, light intensity with 3000-3500 lux, continuous aeration during culture for 10-14 days.
[011] Culturing isochrysis galbana3011 with high lipid content, microalgae cells was firstly placed in uniform electric field with electric intensity of 2x106 V/m for min, and then was placed in uniform magnetic field with magnetic intensity of 50 mT for 20 min; Secondly, the above microalgae (isochrysis galbana 8701) were cultured according to the following parameters of 4-6 g/L of glucose as carbon source, seawater with salinity of 33-34 and pH of 8.2-8.4, culturing temperature with 25±1°C, light intensity with 3000-3500 lux, continuous aeration during culture for 10-14 days.
[012] Compared to existing technology, the invention have the following advantage:
[013] The invention provided a method which used successively uniform electric field and uniform magnetic field on microalgae, and then cultured microalgae with following parameters of 4-6 g/L of glucose as carbon source, seawater with salinity of 33-34 and pH of 8.2-8.4, culturing temperature with 19-26°C, light intensity with 3000 3500 lux, continuous aeration during culture for 10-14 days. This lipid content of above cultured microalgae cells is 40% of microalgae cells dry weight, which is 1.8 times of microalgae lipid content in traditional cultivation methods.
[014] The optimally selected mode contemplated by the applicant for carrying out the invention will be described below in detail.
[015] A method of cultivating microalgae with high lipid content was based on the following steps:
[016] Firstly, microalgae (isochrysis galbana) were placed in a uniform electric field with electric intensity of 1x10 6-2x106 V/m for 10-20 min, and then were placed in a uniform magnetic field with magnetic intensity of 50-100 mT for 10-20min; Secondly, the above microalgae (isochrysis galbana) were cultured according to the following parameters of 4-6 g/L of glucose as carbon source, seawater with salinity of 33-34 and pH of 8.2-8.4, culturing temperature with 19-26°C, light intensity with 3000 3500 lux, continuous aeration during culture for 10-14 days. The microalgae(isochrysis galbana) with high lipid content was obtained. This is a method of cultivating microalgae of high lipid content with low cost and easy operation.
[017] Example 1:
[018] For culturing isochrysis galbana 8701 with high lipid content, microalgae cells was firstly placed in uniform electric field with electric intensity of1x106 V/m for min, and then was placed in uniform magnetic field with magnetic intensity of 100 mT for 15 min; Secondly, the above microalgae (isochrysis galbana 8701) were cultured according to the following parameters of 4-6 g/L of glucose as carbon source, seawater with salinity of 33-34 and pH of 8.2-8.4, culturing temperature with 20±1°C, light intensity with 3000-3500 lux, continuous aeration during culture. On the 10th day of culture, microalgae density reached the highest, which is 1.2 times of microalgae density in traditional cultivation methods; On the 12th day of culture, the lipid content of microalgae cells is the highest with 42.1% of microalgae cells dry weight, which is 1.83 times of microalgae lipid content in traditional cultivation methods.
[019] Example 2:
[020] For culturing isochrysis galbana 3011 with high lipid content, microalgae cells was firstly placed in uniform electric field with electric intensity of 2x106 V/m for min, and then was placed in uniform magnetic field with magnetic intensity of 50 mT for 20 min; Secondly, the above microalgae (isochrysis galbana 3011) were cultured according to the following parameters of 4-6 g/L of glucose as carbon source, seawater with salinity of 33-34 and pH of 8.2-8.4, culturing temperature with 25±1°C, light intensity with 3000-3500 lux, continuous aeration during culture. On the 12th day of culture, the lipid content of microalgae cells is the highest, which is 1.81 times of microalgae lipid content in traditional cultivation methods.
[021] There was provided a method of cultivating microalgae with high lipid content, which was based on the following steps: Firstly, microalgae (isochrysis galbana) were placed in a uniform electric field with electric intensity of 1x10 6-2x106 V/m for 10-20 min, and then were placed in a uniform magnetic field with magnetic intensity of 50-100 mT for 10-20min; Secondly, the above microalgae (isochrysis galbana) were cultured according to the following parameters of 4-6 g/L of glucose as carbon source, seawater with salinity of 33-34 and pH of 8.2-8.4, culturing temperature with 19-26°C, light intensity with 3000-3500 lux, continuous aeration during culture for 10-14 days. The microalgae(isochrysis galbana) with high lipid content was obtained. This is a method of cultivating microalgae of high lipid content with low cost and easy operation.
[022] The method of the present invention may use microalgae(isochrysis galbana 8701) or microalgae (isochrysisgalbana 3011).
[023] The microalgae(isochrysis galbana 8701) cells was firstly placed in uniform electric field with electric intensity of 1x106 V/m for 20min, and then was placed in uniform magnetic field with magnetic intensity of 100 mT for 15 min; Secondly, the above microalgae (isochrysisgalbana 8701) were cultured according to the following parameters of 4-6 g/L of glucose as carbon source, seawater with salinity of 33-34 and pH of 8.2-8.4, culturing temperature with 20±1°C, light intensity with 3000-3500 lux, continuous aeration during culture for 10-14 days. The microalgae(isochrysisgalbana 8701) with high lipid content was obtained.
[024] The microalgae(isochrysis galbana 3011) cells was firstly placed in uniform electric field with electric intensity of 2x106 V/m for 10min, and then was placed in uniform magnetic field with magnetic intensity of 50 mT for 20 min; Secondly, the above microalgae (isochrysisgalbana 8701) were cultured according to the following parameters of 4-6 g/L of glucose as carbon source, seawater with salinity of 33-34 and pH of 8.2-8.4, culturing temperature with 25±1°C, light intensity with 3000-3500 lux, continuous aeration during culture for 10-14 days. The microalgae(isochrysisgalbana 3011) with high lipid content was obtained.
[025] Although the invention has been described with reference to specific examples, it will be appreciated by those skilled in the art that the invention may be embodied in many other forms, in keeping with the broad principles and the spirit of the invention described herein.
[026] The present invention and the described embodiments specifically include the best method known to the applicant of performing the invention. The present invention and the described preferred embodiments specifically include at least one feature that is industrially applicable
Claims (4)
1. A method of cultivating microalgae with high lipid content, which was based on the following steps: Firstly, microalgae (isochrysis galbana) were placed in a uniform electric field with electric intensity of 1x106-2x106 V/m for 10-20 min, and then were placed in a uniform magnetic field with magnetic intensity of 50-100 mT for -20min; Secondly, the above microalgae (isochrysis galbana) were cultured according to the following parameters of 4-6 g/L of glucose as carbon source, seawater with salinity of 33-34 and pH of 8.2-8.4, culturing temperature with 19-26°C, light intensity with 3000-3500 lux, continuous aeration during culture for 10-14 days. The microalgae(isochrysisgalbana) with high lipid content was obtained. This is a method of cultivating microalgae of high lipid content with low cost and easy operation.
2. According to claim 1, here used microalgae(isochrysisgalbana 8701) or microalgae (isochrysisgalbana 3011).
3. According to claim 2, microalgae(isochrysisgalbana 8701) cells was firstly placed in uniform electric field with electric intensity of 1x106 V/m for 20min, and then was placed in uniform magnetic field with magnetic intensity of 100 mT for 15 min; Secondly, the above microalgae (isochrysisgalbana 8701) were cultured according to the following parameters of 4-6 g/L of glucose as carbon source, seawater with salinity of 33-34 and pH of 8.2-8.4, culturing temperature with 20±1°C, light intensity with 3000-3500 lux, continuous aeration during culture for 10-14 days. The microalgae(isochrysisgalbana 8701) with high lipid content was obtained.
4. According to claim 2, microalgae(isochrysisgalbana 3011) cells was firstly placed in uniform electric field with electric intensity of 2x106V/m for 10min, and then was placed in uniform magnetic field with magnetic intensity of 50 mT for 20 min; Secondly, the above microalgae (isochrysisgalbana 8701) were cultured according to the following parameters of 4-6 g/L of glucose as carbon source, seawater with salinity of 33-34 and pH of 8.2-8.4, culturing temperature with 25±1°C, light intensity with 3000-3500 lux, continuous aeration during culture for 10-14 days. The microalgae(isochrysisgalbana 3011) with high lipid content was obtained.
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| AU2020101953A AU2020101953A4 (en) | 2020-08-24 | 2020-08-24 | A method of cultivating microalgae with high oil content |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109536484A (en) * | 2018-11-23 | 2019-03-29 | 江苏大学 | A method of yellow silk algae biomass and metabolin are improved using magnetic field intervention |
| CN114891777A (en) * | 2022-05-19 | 2022-08-12 | 江苏大学 | Device and method for improving microalgae extracellular polysaccharide secretion by high-voltage electric field discharge stimulation |
-
2020
- 2020-08-24 AU AU2020101953A patent/AU2020101953A4/en not_active Ceased
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109536484A (en) * | 2018-11-23 | 2019-03-29 | 江苏大学 | A method of yellow silk algae biomass and metabolin are improved using magnetic field intervention |
| CN109536484B (en) * | 2018-11-23 | 2022-06-21 | 江苏大学 | Method for improving biomass and metabolite of chrysophyceae by magnetic field intervention |
| CN114891777A (en) * | 2022-05-19 | 2022-08-12 | 江苏大学 | Device and method for improving microalgae extracellular polysaccharide secretion by high-voltage electric field discharge stimulation |
| CN114891777B (en) * | 2022-05-19 | 2024-05-14 | 江苏大学 | Device and method for improving secretion of microalgae extracellular polysaccharide by using high-voltage electric field discharge stimulation |
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