AU2013257717A1 - Compositions and methods for the treatment of metabolic syndrome - Google Patents

Abstract

The invention relates to the compounds of formula (I) or its pharmaceutical acceptable salts, as well as polymorphs, solvates, enantiomers, stereoisomers and hydrates thereof. The pharmaceutical compositions comprising an effective amount of compounds of formula (I), and methods for the treatment of metabolic syndrome may be formulated for oral, buccal, rectal, topical, transdermal, transmucosal, intravenous, parenteral administration, syrup, or injection. Such compositions may be used to treatment of hyperuricemia, gout; dyslipidemia, obesity, urea cycle disorders, hyperglycemia, insulin resistance, diabetes mellitus, diabetes insipidus, type 1 diabetes, type 2 diabetes, microvascular complications,.macrovascular complications, lipid disorders, prediabetes., obesity, arrhythmia, myocardial infarction, stroke, neuropathy, renal complications, hypertriglyceridemia, cardiovascular complications, and post prandial hyperglycemia.

Description

WO 2013/167996 PCT/IB2013/050904 1 COMPOSITIONS AND METHODS FOR THE TREATMENT OF M ETABOLIC SYNDROME PRIORITY 100011 The present application claims the benefit of Indian Provisional Patent Application No. 1835/CHE/2012 filed on 10-May-2012, the entire disclosure of which is relied on for all purposes and is incorporated into this application by reference. FIELD OF THE INVENTION 100021 This disclosure generally relates to compounds and compositions for the treatment of metabolic syndrome. More particularly, this invention relates to treating subjects with a pharmaceutically acceptable dose of compounds, crystals, solvates, enantiomer or stereoisomer, esters, salts, hydrates, prodrugs, or mixtures thereof BACKGROUND OF T HE INVENTION 100031 The multifaceted metabolic syndrome is defined as a number of major metabolic disorders that enhances the risk of cardiovascular disease (CVD) - still the most important cause of death in the Western world. It is also known as the insulin resistance syndrome, syndrome X, dysnetabolic syndrome, or the deadly quartet, and is characterized by aberrations in a wide variety of metabolic risk markers such as hyperinsulinemia, impaired glucose metabolism, elevated plasma levels of trhglycerides, decreased levels of high-density lipoprotein cholesterol (HDL-C), raised blood pressure. centrally distributed obesity, impaired endothelial and haemostatic function, and a low grade inflammatory state 100041 Type 2 Diabetes Mellitus (T2DM) is characterized by fisting and postprandial metabolic syndrome and relative insulin insuffici enlcy. If left untreated, then metabolic syndrome may cause long term microvascular and macrovascular complications, such as nephropathv, neuropathy, retinopathy, and atherosclerosis. This disease causes significant WO 2013/167996 PCT/IB2013/050904 2 morbidity and mortality at considerable expense to patients, their families and society. The incidence of T2DM worldwide is now increasing at more rapid rates in Africa, Asia and South America than in Europe or the U.S. Thus, T2DM is now considered worldwide epidemic. 100051 Oxidative stress has long been associated with the late complications of diabetes, and has been implicated in their etiology. The reactive oxygen intermediates, produced in mitochondria, peroxisomes, and the cytoso, are scavenged by cellular defending systems, including enzymatic (ex, superoxide di smutase, glutathione peroxidase GPx, glutathione reductase and catalase) and nonenzymatic antioxidants (ex. glutathi one G-SH, thioredoxin, lipoic acid, ubiquinol, albumin, uric acid, flavonoids, vitamins A, C and. E, etc.). Some are located in cell membranes, others in the cytosol, and others in the blood plasma. In metabolic syndrome, an altered oxidative metabolism is a consequence either of the chronic exposure to hyperglycemia or of the absolute or relative insulin deficit; insulin regulates several reactions involved in oxido-reductive metabolism. Despite strong experimental evidence indicating that oxidative stress may determine the onset and progression of late-diabetic complications controversy exists about whether the increased oxidative stress is merely associative rather than causal in metabolic syndrome. 100061 Managing acute pathology of often relies on the addressing underlying pathology and symptoms of the disease. There is currently a need in the art for new compositions to treatment or delay of the onset of diabetes related metabolic syndrome and its associated complications progression. SUMMARY OF THE INVENTION 100071 The present invention provides compounds, compositions containing these compounds and methods for using the same to treat, prevent and/or ameliorate the effects of the conditions such as metabolic syndrome.

WO 2013/167996 PCT/IB2013/050904 3 100081 The invention herein provides compositions comprising of formula I or pharmaceutical acceptable salts thereof The invention also provides pharmaceutical compositions comprising one or more compounds of formula I or intermediates thereof and one or more of pharmaceutically acceptable carriers, vehicles or diluents, These compositions may be used in the treatment of metabolic syndrome and its associated complications. 0 -1R~ j 1 R 4 Fa R1 Formula 1 100091 In certain embodiments, the present invention relates to the compounds and compositions of formula 1, or pharmaceutically acceptable salts thereof, 0

CF

3 Formula I Wherein,

R

1 independently represents halogen, ~F, 14, ~Cl, -D or -Br; WO 2013/167996 PCT/IB2013/050904 4 H OAN R2 independently represents -H, -D, or R" independently represents H D, O 0 H2 O 0 O O 0 - -\ - N H2' NH2 0 H H NNH. NH N H N N WO 2013/167996 PCT/IB2013/050904 5 0 O OH 0 HH N / NN N 01r H

R

4 independent y represents

NHCOCH

2 CO0 2 HgCONH2 OH

OCOCH

3 0 XOH t 0 HN eO H N OH 3 H OHH S NH2 0N Ci3 0 HO O O-

,

WO 2013/167996 PCT/IB2013/050904 6 0 0 0 N\ N NT N 0 NH NH NN NN N / H H

N/

WO 2013/167996 PCT/IB2013/050904 7 0 OH HHN NH H H HO OH OHO H S OHO HO HOH, N HS NH,, H O NH N HH O0 N H 0 NN N OH O 0O O 0 r b WO 2013/167996 PCT/IB2013/050904 8 a is independently 2,3 or 7; each b is independently 3, 5 or 6; e is independently 1, 2 or 6; c and d are each independently H D, -OH, -OD, C Co-alkyI, -NH 2 or -COCH [0010] In the illustrative embodiments, examples of compounds of formula I are as set forth below: O 00 (1-) 0FCF CCI (-1) WO 2013/167996 PCT/IB2013/050904 9 HO 0 0 CF~ ci (1-3) [0011] Herein the application also provides a kit comprising any of the pharmaceutical compositions disclosed herein, The kit may comprise instructions for use in the treatment of metabolic syndrome or its related complications. [0012J The application also discloses a pharmaceutical composition comprising a pharmaceutically acceptable carrier and any of the compositions herein. In some aspects, the pharmaceutical composition is formulated for systemic administration, oral administration, sustained release., parenteral. administration, injection, subdernal administration, or transdermal administration. 100131 lerein, the application additionally provides kits comprising the pharmaceutical compositions described herein. The kits may further comprise instructions for use in the treatment of metabolic syndrome or its related complications. 100141 The compositions described herein have several uses. The present application provides, for example, methods of treating a patient suffering from metabolic syndrome or its related complications manifested from metabolic conditions or disorders, metabolic syndrome, chronic diseases or disorders; Hyperinsulinemia, Insulin resistance, Glucose intolerance, Fiepatology, Cancer, Respiratory, Hematological, Orthopedic, Cardiovascular, Renal, Skin, Vascular or Ocular complications.

WO 2013/167996 PCT/IB2013/050904 10 STAIEIS) DESCRIPiN' OF THE INVENTION Definitions 100151 As used herein, the following terns and phrases shall have the meanings set forth below, Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art, 10016j The compounds of the present invention can be present in the form of pharmaceutically acceptable salts. The compounds of the present invention can also be present in the form of pharmaceutically acceptable esters (ie, the methyl and ethyl esters of the acids of formula I to be used as prodrugs). The compounds of the present invention can also be solvated, i.e. hydrated. The solvation can be affected in the course of the manufacturing process or can take place i.e. as a consequence of hygroscopic properties of an initially anhydrous compound of formula I (hydration). [00.1,71 Compounds that have the same molecular formnaula but differ in the nature or sequence of bonding of their atoms or the arrangement of their atoms in space are termed isomerss." Isomers that differ in the arrangement of their atoms in space are termed "stereoisomers." Diastereorners are stereoisomers with opposite configuration at one or more chiral centers which are not enantiomers. Stereoisomers bearing one or more asymmetric centers that are non- superimposable mirror i mages of each other are termed "enantiomers." When a compound has an asynnnetric center, for example, if a carbon atom is bonded to four different groups, a pair of enantiomers is possible. An enantiomer can be characterized by the absolute configuration of its asymmnietric center or centers and is described by the R- and S-sequencing riles of Cahn, Ingold and Prelog. or by the manner in which the molecule rotates the plane of polarized light and designated as dextrorotatorv or levorotatory (i.e., as (+) or (-)-isomers respectively). A chiral compound can exist as either individual enantiomer or as a mixture thereof. A mixture containing equal proportions of the enantiomers is called a racemicc mixture" WO 2013/167996 PCT/IB2013/050904 11 100181 As used herein, the term "metabolic condition" refers to an Inbom errors of metabolism (or genetic metabolic conditions) are genetic disorders that result from a defect in one or more metabolic pathways; specifically, the function of an enzyme is affected and is either deficient or completely absent. [0019J In some embodiments, a molecular conjugate comprises of compounds selected from the group consisting of R-ipoic acid (CAS No. 1200-22-2), salsalate (CAS No. 552-94-3), acetylcysteine (CAS No. 616-91-1), Eicosapentaenoic acid (CAS No. 10417 94-4), Docosahexaenoic acid (CAS No. 6217-54-5), [00201 The term polymorphh" as used herein is art-recognized and refers to one crystal structure of a given compound. [00211 The phrases "parenteral administration" and "administered parenterally" as used herein refer to modes of administration other than enteral and topical administration, such as injections, and include without limitation intravenous, intramuscular, intrapleural, intravascular, intrapericardial, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradennal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intra articular, suLbcapsular, subarachnoid, intraspinal and intrastenial injection and infusion, 10022] A "patient," "subject," or "host" to be treated by the subject method may mean either a human or non-human animal, such as primates, mammals, and vertebrates. 10023] The phrase "pharmaceutically acceptable" is art-recognized. In certain embodiments, the term includes compositions, polymers and other materials and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of mammals, human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio.

WO 2013/167996 PCT/IB2013/050904 12 100241 The phrase "pharmaceutically acceptable carrier" is art-recognized, and includes, for example, pharmaceutically acceptable materials, compositions or vehicles., such as a liquid or solid filler, diluent, solvent or encapsulating material involved in carrying or transporting any subject composition, from one organ, or portion of the body, to another organ, or portion of the body. Each carrier must be "acceptable" in the sense of being compatible with the other ingredients of a subject composition and. not injurious to the patient. In certain embodiments, a pharmaceutically acceptable carrier is non-pyrogenic, Some examples of materials which may serve as pharmaceutically acceptable carriers include. (I) sugars, such as lactose, glucose and sucrose; (2) starches, such as corn starch and potato starch; (3) cellulose, and its derivatives, such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; (4) powdered tragacanth; (5) malt; (6) gelatin; (7) talc; (8) cocoa butter and suppository waxes; (9) oils, such as peanut oil, cottonseed oil, sunflower oil, sesame oil, olive oil, com oil and soybean oil; (10) glycols, such as propylene glycol; (1.1) polyols, such as glycerin, sorbitol, rannitol and polyethylene glycol; (12) esters, such as ethyl oleate and ethyl laurate; (13) agar; (14) buffering agents, such as magnesium hydroxide and aluminum hydroxide; (15) al ginic acid; (16) pyrogen-free water; (17) isotonic saline; (18) Ringer's solution; (19) ethyl alcohol; (20) phosphate buffer solutions; and (21) other non-toxic compatible substances employed in pharmaceutical formulations. [0025J The term "prodrug" is intended to encompass compounds that, under physiological conditions, are converted into the therapeutically active agents of the present invention. A common method for making a prodrug is to include selected moieties that are hydrolyzed under physiological conditions to reveal the desired molecule. In other embodiments, the prodrug is converted by an enzymatic activity of the host animal, [00261 The term "prophylactic or therapeutic" treatment is art-recognized and includes administration to the host of one or more of the subject compositions. If it is administered prior to clinical manifestation of the unwanted condition (e. disease or other unwanted state of the host animal) then the treatment is prophylactic, ie, it protects WO 2013/167996 PCT/IB2013/050904 13 the host against developing the unwanted condition, whereas if it is administered after manifestation of the unwanted condition, the treatment is therapeutic, (ie, it is intended to diminish, ameliorate, or stabilize the existing unwanted condition or side effects thereof). 100271 The term "predicting" as used herein refers to assessing the probability related diseases patient will suffer from abnormalities or complication and/Or terminal platelet aggregation. or failure and/or death (i.e. mortality) within a defined time window (predictive window) in the future. The mortality may be caused by the central nervous system. or complication. The predictive window is an interval in which the subject will develop one or more of the said complications according to the predicted probability. The predictive window may be the entire remaining lifespan of the subject upon analysis by the method of the present invention. [0028] The term "treating" is art -recognized and includes preventing a disease, disorder or condition from occurring in an animal which may be predisposed to the disease., disorder and/or condition but has not yet been diagnosed as having it; inhibiting the disease, disorder or condition, e*g., impeding its progress; and relieving the disease, disorder, or condition, e.g., causing regression of the disease, disorder and/or condition. Treating the disease or condition includes ameliorating at least one symptom of the particular disease or condition, even if the underlying pathophysiology is not affected, such as treating the metabolic syndrome, hyperuricemia, gout, dyslipidemia, obesity, urea cycle disorders, hyperglycemia, insulin resistance, diabetes mellitus. diabetes insipidus, type 1 diabetes, type 2 diabetes, microvascular complications, macrovascular complications, lipid disorders, prediabetes, obesity, arrhythmia, myocardial infarction, stroke, neuropathy, renal complications, hypertriglyceridemia, cardiovascular complications, and post prandial hyperglycemia of a subject by administration of an agent even though such agent does not treat the cause of the condition. The term "treating" "treat" or "treatment" as used herein includes curative, preventative (e g., prophylactic), adjunct and palliative treatment.

WO 2013/167996 PCT/IB2013/050904 14 10029 j The phrase "therapeutically effective amount" is an art-recognized term. In certain embodiments, the term refers to an amount of a salt or composition disclosed herein that produces some desired effect at a reasonable benefit/risk ratio applicable to any medical treatment. In certain embodiments, the term refers to that amount necessary or sufficient to eliminate or reduce medical symptoms for a period of time. The effective amount may vary depending on such factors as the disease or condition being treated, the particular targeted constructs being administered, the size of the subject, or the severity of the disease or condition. One of ordinary skill in the art may empirically detemine the effective amount of a particular composition without necessitating undue experimentation. f0030] In certain embodiments, the pharmaceutical compositions described herein are formulated in a manner such that said compositions will be delivered to a patient in a therapCutically effective amount, as part of a prophylactic or therapeutic treatment, The desired amount of the composition to be administered to a patient will depend on absorpton inactivation, and excretion rates of the drug as well as the delivery rate of the salt% and compositions from the subject compositions. It is to be noted that dosage values may also vary with the severity of the condition to be alleviated. It is to be further understood that for any particular subject, specific dosage regimens should be adjusted over time according to the individual need and the professional judgment of the person administering or supervising the administration of the compositions. Typically, dosing will be determined using techniques known to one skilled in the art. 100311 Additionally, the optimal concentration and/or quantities or amounts of any particular salt or composition may be adjusted to accommodate variations in the treatment parameters. Such treatment parameters include the clinical use to which the preparation is put, e.g., the site treated, the type of patient, e.g., human or non-human, adult or child, and the nature of the disease or condition. [0032J In certain embodiments, the dosage of the subject compositions provided herein may be determined by reference to the plasma concentrations of the therapeutic WO 2013/167996 PCT/IB2013/050904 15 composition or other encapsulated materials. For example, the maximum plasma concentration (Cmax) and the area under the plasma concentration-ti me curve from time 0 to infinity may be used. 100331 When used with respect to a pharmaceutical composition or other material, the term "sustained release" is art-recognized, For example, a subject composition which releases a substance over time may exhibit sustained release characteristics, in contrast to a bolus type administration in which the entire amount of the substance is made biologically available at one time. For example, in particular embodiments, upon contact with body fluids including blood, spinal fluid, mucus secretions, lymph or the like, one or more of the pharmaceutically acceptable excipients may undergo gradual or delayed degradation (e.g., through hydrolysis) with concomitant release of any material incorporated therein, e.g. an therapeutic and/or biologically active salt and/or composition, for a sustained or extended period (as compared to the release from a bolus). This release may result in prolonged delivery of therapeutically effective amounts of any of the therapeutic agents disclosed herein. 100341 The phrases "systemic administration," "administered systenically," "'peripheral administration" and "administered peripherally" are art-recognized, and include the administration of a subject composition, therapeutic or other material at a site remote from the disease being treated. Administration of an agent for the disease being treated, even if the agent is subsequently distributed systemically, may be termed "local" or "topical" or "regional" administration, other than directly into the central nervous system, eg., by subcutaneous administration, such that it enters the patient's system and, thus, is subject to metabolism and other like processes. [00351 The phrase "therapeutically effective amount" is an art-recognized term. In certain embodiments, the term refers to an amount of a salt or composition disclosed herein that produces some desired effect at a reasonable benefit/risk ratio applicable to any medical treatment. In certain embodiments, the term refers to that amount necessary or sufficient to eliminate or reduce medical symptoms for a period of time. The effective WO 2013/167996 PCT/IB2013/050904 16 amount may vary depending on such factors as the disease or condition being treated, the particular targeted constructs being administered, the size of the subject, or the severity of the disease or condition. One of ordinary skill in the art may empirically determine the effective amount of a particular composition without necessitating undue experimentation. [0036J The present disclosure also contemplates prodrugs of the compositions disclosed herein, as well as pharmaceutically acceptable salts of said prodrugs. 100371 This application also discloses a pharmaceutical composition comprising a pharmaceutically acceptable carrier and the composition of a compound of Formula I may be formulated for systemic or topical or oral administration. The pharmaceutical composition may be also formulated for oral administration, oral solution, injection, subdermal administration, or transdermal administration. The pharmaceutical composition may further comprise at least one of a pharmaceutically acceptable stabilizer, diluent, surfactant, filler, binder, and lubricant. 100381 In many embodiments, the pharmaceutical compositions described herein will incorporate the disclosed compounds and compositions (Formula 1) to be delivered in an amount sufficient to deliver to a patient a therapeutically effective amount of a compound of formula I or composition as part of a prophylactic or therapeutic treatment. The desired concentration of formula I or its pharmaceutical acceptable salts will depend on absorption, inactivation, and excretion rates of the drug as well as the delivery rate of the salts and compositions from the subject compositions. It is to be noted that dosage values may also vary with the severity of the condition to be alleviated. It is to be further understood that for any particular subject, specific dosage regimens should be adjusted over time according to the individual need and the professional judgment of the person administering or supervising the administration of the compositions. Typically, dosing will be determined using techniques known to one skilled in the art.

WO 2013/167996 PCT/IB2013/050904 17 10039j Additionally, the optimal concentration and/or quantities or amounts of any particular compound of formula I may be adjusted to accommodate variations in the treatment parameters. Such treatment parameters include the clinical use to which the preparation is put, e.g., the site treated, the type of patient, e.g.. human or non-human, adult or child, and the nature of the disease or condition. [0040J The concentration and/or amount of any compound of formula I may be readily identified by routine screening in animals, e.g,, rats, by screening a range of concentration and/or amounts of the material in question using appropriate assays. Known methods are also available to assay local tissue concentrations, diffusion rates of the salts or compositions, and local blood flow before and after administration of therapeutic fornmlations disclosed herein. One such method is microdialysis, as reviewed by T. E. Robinson et al, 1991, microdialysis in the neurosciences, Techniques, volume 7, Chapter . The methods reviewed by Robinson may be applied, in brief, as follows. A microdialysis loop is placed in situ in a test animal, Dialysis fluid is pumped through the loop. When compounds with formula I such as those disclosed herein are injected adjacent to the loop, released drugs are collected in the dialysate in proportion to their local tissue concentrations. The progress of diffusion of the salts or compositions may be determined thereby with suitable calibration procedures using known concentrations of salts or compositions. [0041] In certain embodiments, the dosage of the subject compounds of formula I provided herein may be determined by reference to the plasma concentrations of the therapeutic composition or other encapsulated materials. For example, the maximum plasma concentration (Cmax) and the area under the plasma concentration-time curve from time 0 to infinity may be used. 100421 Generally, in carrying out the methods detailed in this application, an effective dosage for the compounds of Formulas I is in the range of about 0.01 mg/kg/day to about 100 mg/kg/day in single or divided doses, for instance 0.01 mg/kg/day to about 50 mg/kg/day in single or divided doses. The compounds of Formulas I may be administered WO 2013/167996 PCT/IB2013/050904 18 at a dose of, for example, less than 0,2 mg/kg/day, 0.5 mg/kg/day, L 0 mg/kg/day, 5 mg/kg/day, 10 mg/kg/day, 20 mg/kg/day, 30 mg/kg/day, or 40 mg/kg/day. Compounds of Formula I may also be administered to a human patient at a dose of, for example, between 0. 1 mg and 1000 mg, between 5 mg and 80 mg, or less than 1,0, 9,0, 12.0, 20.0, 50.0, 75.0, 100, 300, 400, 500, 800, 1000, 2000, 5000 mg per day. In certain embodiments, the compositions herein are administered at an amount that is less than 95%,90%,80%,70%,.60%, 50%,40%, 30%, 20%, or 10% of the compound of formula I required for the same therapeutic benefit. 100431 An effective amount of the compounds of formula I described herein refers to the amount of one of said salts or compositions which is capable of inhibiting or preventing a disease. 100441 An effective amount may be sufficient to prohibit, treat, alleviate, ameliorate, halt, restrain, slow or reverse the progression, or reduce the severity of a complication resulting from nerve damage or denyelization and/or elevated reactive oxidative nitrosative species and/or abnormali ties in physiological homeostasis', in patients who are at risk for such complications As such, these methods include both medical therapeutic (acute) and/or prophylactic (prevention) administration as appropriate. The amount and timing of compositions administered will, of course, be dependent on the subject being treated, on the severity of the affliction, on the manner of administration and on the judgment of the prescribing physician. Thus, because of patient-to-patient variability, the dosages given above are a guideline and the physician may titrate doses of the drug to achieve the treatment that the physician considers appropriate for the patient. In considering the degree of treatment desired, the physician must balance a variety of factors such as age of the patient, presence of preexisting disease, as well as presence of other diseases. f0045] The compositions provided by this application may be administered to a subject in need of treatment by a variety of conventional routes of administration, including orally, topically, parenterally, e.g., intravenously, subcutaneously or intramedullary.

WO 2013/167996 PCT/IB2013/050904 19 Further, the compositions may be administered intranasally, as a rectal suppository, or using a "flash" formulation, i.e., allowing the medication to dissolve in the mouth without the need to use water. Furthermore, the compositions may be administered to a subject in need of treatment by controlled release dosage forms, site specific drug delivery, transdermal drug delivery, patch (active/passive) mediated drug delivery, by stereotactic injection, or in nanoparticles. [00461 The compositions may be administered alone or in combination with pharmaceutical acceptable carriers, vehicles or diluents, in either single or multiple doses. Suitable pharmaceutical carriers, vehicles and diluents include inert solid diluents or fillers, sterile aqueous solutions and various organic solvents, The pharmaceutical compositions formed by combining, the compositions and the pharmaceutically acceptable carriers, vehicles or diluents are then readily administered in a variety of dosage fBorms such as tablets, powders, lozenges, syrups, injectable solutions and the like. These pharmaceutical compositions can, if desired, contain additional ingredients such as flavorings., binders. excipients and the like. Thus, for purposes of oral administration, tablets containing various excipients such as L-arginine, sodium citrate, calcium carbonate and calcium phosphate may be employed along with various disintegrates such as starch, alginic acid and certain complex silicates, together with binding agents such as polyvi nylpyrroli done, sucrose, gelatin and acacia. Additionally, lubricating agents such as magnesium stearate, sodium laury1 sulfate and talc are often useful for tabletting purposes. Solid compositions of a similar type may also be employed as fillers in soft and hard filled gelatin capsules. Appropriate materials for this include lactose or milk sugar and high molecular weight polyethylene glycols. When aqueous suspensions or elixirs are desired for oral administration, the essential active ingredient therein may be combined with various sweetening or flavoring agents, coloring matter or dyes and, if desired, emulsifying or suspending agents, together with diluents such as water, ethanol, propylene glycol, glycerin and combinations thereof. The compounds of formula I may also comprise enterically coated comprising of various excipients, as is well known in the pharmaceutical art- WO 2013/167996 PCT/IB2013/050904 20 10047 j For parenteral administration, solutions of the compositions may be prepared in (for example) sesame or peanut oil, aqueous propylene glycol, or in sterile aqueous solutions may be employed. Such aqueous solutions should be suitably buffered if necessary and the liquid diluent first rendered isotonic with sufficient saline or glucose. These particular aqueous solutions are especially suitable for intravenous, intramuscular, subcutaneous and intraperitoneal administration, In this connection, the sterile aqueous media employed are all readily available by standard techniques know to those skilled in the art. 100481 The formulations, for instance tablets, may contain e.g 10 to 100, 50 to 250, 150 to 500 mg, or 350 to 800 mng e g. 10, 50, 100, 300, 500, 700, 800 mg of the compounds of formula I disclosed herein, for instance, compounds of formula I or pharmaceutical acceptable salts of a compounds of Formula 1, [00491 Generally, a composition as described herein may be administered orally, or parerterally (e g, intravenous, intramuscular, subcutaneous or intram edullary)s Topical administration may also be indicated, for example, where the patient is suffering from gastrointestinal disorder that prevent oral administration, or whenever the medication is best applied to the surface of a tissue or organ as determined by the attending physician. Localized administration may also be indicated, for example, when a high dose is desired at the target tissue or organ. For buccal administration the active composition may take the form of tablets or lozenges formulated in a conventional manner. 100501 The dosage administered will be dependent upon the identity of the metabolic disease; the type of host involved, including its age, health and weight; the kind of concurrent treatment, if any; the frequency of treatment and therapeutic ratio. 100511 Illustratively, dosage levels of the administered active ingredients are: intravenous, 0. 1 to about 200 mg/kg; intramuscular, I to about 500 mg/kg; orally, 5 to about 1000 mg/kg; intranasal instillation, 5 to about 1000 mg/kg; and aerosol, 5 to about 1000 mg/kg of host body weight.

WO 2013/167996 PCT/IB2013/050904 21 100521 Expressed in terms of concentration, an active ingredient can be present in the compositions of the present invention for localized use about the cutis, intranasally, pharyngolaryngeally, bronchially, intravaginally, rectally, or ocularly in a concentration of from about 0.01 to about 50% w/w of the composition; preferably about I to about 20% w/w of the composition; and for parenteral use in a concentration of from about 0.05 to about 50% wv of the composition and preferably from about 5 to about 20% w/v. 100531 The compositions of the present invention are preferably presented for administration to humans and animals in unit dosage forms, such as tablets, capsules, pills, powders, granules, suppositories, sterile parenteral solutions or suspensions, sterile non-parenteral solutions of suspensions, and oral solutions or suspensions and the like, containing suitable quantities of an active ingredient. For oral administration either solid or fluid unit dosage forms can be prepared. 100541 As discussed above, the tablet core contains one or more hydrophilic polymers. Suitable hydrophilic polymers include, but are not limited to, water swellable cellulose derivatives, polvalkylen e glycols, thermoplastic polyalkyl ene oxides, acrylic polymers, hydrocolloids, clays, gelling starches, swelling cross-linked polymers, and mixtures thereof Examples of suitable water swellable cellulose derivatives include, but are not limited to, sodium carboxymethyl cellulose, cross-linked hydroxypropylcell lose, hydroxypropyl cellulose (JPC), hydroxypropylrnethylcellulose (HPMC), hydroxyisopropyIcellulose, hydroxybutyIcellulose, hydroxyphenylcellulose, hydroxyethylcellulose (HEC), hydroxypentyleellulose, hydroxypropy I ethy lcellulose, hvdroxvpropylbutylcellul ose, and hydroxypropyl ethyl cellulose, and mixtures thereof. Examples of suitable polyalkylene glycols include, but are not limited to, polyethylene glycol, Examples of suitable thermoplastic polyalkylene oxides include, but are not limited to, polyethylene oxide.), Examples of suitable acrylic polymers include, but are not limited to, potassium methacrylatedivinylbenzene copolymer, polymethylm ethacrylate, high-molecular weight crosslinked acrylic acid homopolymers and copolymers such as those commercially available from Noveon Chemicals under the WO 2013/167996 PCT/IB2013/050904 22 tradename CARBOPOL " . Examples of suitable hydrocolloids include, but are not limited to, alginates, agar, guar gum, locust bean gum, kappa carrageenan, iota carrageenan, tara, gum arabic, tragacanth, pectin, xanthan gum, gellan gum, maltodextrin, galactomannan, pusstulan, laminarin, scleroglucan, gum arabic, inulin, pectin, gelatin, whelan, rhamsan, zooglan, methylan, chitin, cyclodextlin, chitosan, and mixtures thereof Examples of suitable clays include, but are not limited to, smectites such as bentonite, kaolin, and laponite; magnesium trisilicate; magnesium aluminum silicate; and mixtures thereof Examples of suitable gelling starches include, but are not limited to, acid hydrolyzed starches, swelling starches such as sodium starch glycolate and derivatives thereof, and mixtures thereof Examples of suitable swelling cross-linked polymers include, but are not limited to., cross-linked polyvinyl pyrrolidone, cross-linked agar, and cross-inked carboxymethylcellulose sodium, and mixtures thereof 100551 The carrier may contain one or more suitable excipients for the formulation of tablets. Examples of suitable excipients include, but are not limited to, fillers, adsorbents, binders, disi ntegrants, lubricants, glidants, release-modifying excipients, superdisintegrants, antioxidants, and mixtures thereof 100561 Suitable binders include, but are not limited to, dry binders such as polyvinyl pyrrolidone and iydroxypropylin ethylcellulose; wet binders such as water-soluble polymers, including hydrocolloids such as acacia, alginates, agar, guar gum, locust bean, carrageenan, carboxymetlcellulose. tara, gum arabic, tragacanth, pectin, xanthan, gellan, gelatin, maltodextrin, galactomannan, pusstulan, laninarin, scleroglucan, inulin, whelan, rham san, zooglan, methylan, chitin, cyclodextrin, chitosan, polyvinyl pyrrolidone, cellulosics, sucrose, and starches; and mixtures thereof Suitable disintegrants include, but are not limited to, sodium starch glycolate, cross-linked polyvinylpyrrolidone, cross-linked carboxymethyleellulose, starches, microcrystalli ne cellulose, and nii xtures thereof 100571 Suitable lubricants include, but are not limited to, long chain fatty acids and. their salts, such as magnesium stearate and stearic acid, tale, glycerides waxes, and mixtures WO 2013/167996 PCT/IB2013/050904 23 thereof Suitable glidants include, but are not limited to, colloidal silicon dioxide. Suitable release-modiying excipients include, but are not limited to, insoluble edible materials, pH-dependent polymers, and mixtures thereof [00581 Suitable insoluble edible materials for use as release-modifying excipients include, but are not limited to, water-insoluble polymers and low-melting hydrophobic materials, copolymers thereof, and mixtures thereof Examples of suitable water insoluble polymers include, but are not limited to, ethylcellulose, polyvinyl alcohols, polyvinyl acetate, polycaprolactones, cellulose acetate and its derivatives, acrylates, methacrylates., acrylic acid copolymers, copolymers thereof, and mixtures thereof Suitable low-melting hydrophobic materials include, but are not limited to, fats, fatty acid esters, phospholipids, waxes, and mixtures thereof Examples of suitable fats include, but are not limited to, hydrogenated vegetable oils such as for example cocoa butter, hydrogenated paln kernel oil, hydrogenated cottonseed oil, hydrogenated sunflower oil, and hydrogenated soybean oil, free fatt acids and their salts, and mixtures thereof Examples of suitable fatty acid esters include, but are not limited to, sucrose fatty acid esters, mono-, di-, and triglycerides, glyceryl behenate, glyceryl palmitostearate, glyceryl monostearate, glyceryl tristearate, glyceryl trilaurylate, glyceryl myristate, GlycoWax 932, lauroyl macrogol-32 glycerides, stearoyl macrogol-32 glycerides, and mixtures thereof Examples of suitable phospholipids include phosphotidyl choline, phosphotidyl serene, phosphotidyl enositol, phosphotidic acid, and mixtures thereof Examples of suitable waxes include, but are not limited to, carnauba wax, spermaceti wax, beeswax, candelilla wax, shellac wax, microcrystalline wax, and paraffin wax; fat-containing mixtures such as chocolate, and mixtures thereof Examples of super disintegrants include, but are not limited to, croscarmellose sodium, sodium starch glycolate and cross linked povidone (crospovidone). In one embodiment the tablet core contains up to about 5 percent by weight of such super disintegrant. [0059] Examples of antioxidants include, but are not limited to, tocopherols, ascorbic acid, sodium pyrosulfite, butyihydroxytoluene, butylated hydroxyani sole, edetic acid, and edetate salts, and mixtures thereof Examples of preservatives include, but are not limited WO 2013/167996 PCT/IB2013/050904 24 to, citric acid, tartaric acid, lactic acid, malic acid, acetic acid, benzoic acid, and sorbic acid, and mixtures thereof 100601 In one embodiment, the immediate release coating has an average thickness of at least 50 microns, such as from about 50 microns to about 2500 microns e.g.. from about 250 microns to about 1000 microns. In embodiment, the immediate release coating is typically compressed at a density of more than about 0.9 g/cc, as measured by the weight and volume of that specific layer, 100611 In one embodiment, the immediate release coating contains a first portion and a second portion, wherein at least one of the portions contains the second pharnaceutically active agent. In one embodiment, the portions contact each other at a center axis of the tablet. In one embodiment, the first portion includes the first phannaceutically active agent and the second portion includes the second pharnaceutically active agent. 100621 In one embodiment, the first portion contains the first pharmaceutically active agent and the second portion contains the second pharmaceutically active agent. In one embodiment, one of the portions contains a third pharmaceutically active agent. In one embodiment one of the portions contains a second immediate release portion of the same pharmaceutically active agent as that contained in the tablet core 100631 In one embodiment, the outer coating portion is prepared as a dry blend of materials prior to addition to the coated tablet core. In another embodiment the outer coating portion is included of a dried granulation including the pharmaceutically active agent. 100641 Formulations with different drug release mechanisms described above could be combined in a final dosage form containing single or multiple units. Examples of multiple units include multilayer tablets, capsules containing tablets, beads, or granules in a solid or liquid form. Typical, immediate release formulations include compressed tablets, gels, films, coatings, liquids and particles that can be encapsulated, for example, WO 2013/167996 PCT/IB2013/050904 25 in a gelatin capsule. Many methods for preparing coatings, covering or incorporating drgs, are known in the art. 100651 The immediate release dosage, unit of the dosage form, i.e., a tablet, a plurality of drug-containing beads, granules or particles, or an outer layer of a coated core dosage form, contains a therapeutically effective quantity of the active agent with conventional pharmaceutical excipients. The immediate release dosage unit may or may not be coated, and may or may not be admixed with the delayed release dosage unit or units (as in an encapsulated mixture of immediate release drug-containing granules, particles or beads and delayed release drug-containing granudes or beads). [0066] Extended release formulations are generally prepared as diffusion or osmotic systems, for example, as described in "Remington-The Science and Practice of Phamacy", 20th. Ed., Lippincott Williams & Wilkins, Baltimore, MI., 2000). A diffusion system typically consists of one of two types of devices, reservoir and matrix, which are wellknown and described in die art. The matrix devices are generally prepared by compressing the drug with a slowly dissolving polymer carrier into a tablet form. 100671 An immediate release portion can be added to the extended release system by means of either applying an immediate release layer on top (f the extended release core; using coating or compression processes or in a multiple unit system such as a capsule containing extended and immediate release beads. [0068J Delayed release dosage formulations are created by coating a solid dosage form with a film of a polymer which is insoluble in the acid environment of the stomach, but soluble in the neutral environment of small intestines, The delayed release dosage units can be prepared, for example, by coating a drug or a drug-containing composition with a selected coating material. The drug-containing composition may be a tablet for incorporation into a capsule, a tablet for use as an inner core in a "coated core" dosage form, or a plurality of dnug-containing beads, particles or granules, for incorporation into either a tablet or capsule.

WO 2013/167996 PCT/IB2013/050904 26 100691 A pulsed release dosage form is one that mimics a multiple dosing profile without repeated dosing and typically allow s at least a twofold reduction in dosing frequency as compared to the drug presented as a conventional dosage form (e.g., as a solution or prompt drug-releasing, conventional solid dosage form). A pulsed release profile is characterized by a time period of no release (lag time) or reduced release followed by rapid drug release. [00701 Each dosage form contains a therapeutically effective amount of active agent In one embodiment of dosage forms that mimic a twice daily dosing profile, approximately 30 wt. % to 70 wt. %, preferably 40 wt. % to 60 wt. %, of the total amount of active agent in the dosage form is released in the initial pulse, and, correspondingly approximately 70 wt. % to 3.0 vt. %, preferably 60 wt. % to 40 wt. %, of the total amount of active agent in the dosage four is released in the second pulse. For dosage forms mimicking the twice daily dosing profile, the second pulse is preferably released approximately 3 hours to less than 14 hours, and more preferably approximately 5 hours to 12 hours, following administration. 100711 Another dosage form contains a compressed tablet or a capsule having a drug containing inmediate release dosage unit, a delayed release dosage unit and an optical second delayed release dosage unit. In this dosage form, the immediate release dosage unit contains a plurality of beads, granules particles that release drug substantially immediately following oral administration to provide an initial dose. The delayed release dosage unit contains a plurality of coated beads or granules, which release drug approximately 3 hours to 14 hours following oral administration to provide a second dose. 100721 For purposes of transdermal (e g, topical) administration, dilute sterile, aqueous or partially aqueous solutions (usually in about 0,1% to 5% concentration), otherwise similar to the above parenteral solutions, may be prepared.

WO 2013/167996 PCT/IB2013/050904 27 10073j Methods of preparing various pharmaceutical compositions with a certain amount of one or more compounds of formula I or other active agents are known, or will be apparent in light of this disclosure, to those skilled in this art. For examples of methods of preparing pharmaceutical compositions, see Remington's Pharmaceutical Sciences, Mack Publishing Company, Easton, Pa., 19th Edition (1995). [0074J In addition, in certain embodiments, subjet compositions of the present application maybe lyophilized or subjected to another appropriate drying technique such as spray drying. The subject compositions may be administered once, or may be divided into a number of smaller doses to be administered at varying intervals of time, depending in part on the release rate of the compositions and the desired dosage. [00751 Formulations useful in the methods provided herein include those suitable for oral, nasal., topical (including buccal and sublingual), rectal, vaginal, aerosol and/or parenteral administration. The formulations may conveniently be presented in unit dosage form and may be prepared by any methods well known in the art of pharacy. The amount of a subject composition which may be combined with a carrier material to produce a single dose may vary depending upon the subject being treated, and the particular mode of administration. [00761 Mdethods of preparing these formulations or compositions include the step of bringing into association subject compositions with the carrier and, optionally, one or more accessory ingredients, In general, the formulations are prepared by unifornly and intimately bringing into association a subject composition with liquid carriers, or finely divided solid carriers, or both, and then, if necessary, shaping the product. [00771 The compounds of formula I described herein may be administered in inhalant or aerosol formulations. The inhalant or aerosol formulations may compri se one or more agents, such as adjuvants, diagnostic agents, imaging agents, or therapeutic agents useful in inhalation therapy. The final aerosol fornulation may for example contain 0.005-90% WO 2013/167996 PCT/IB2013/050904 28 w/w, for instance 0,005-50%, 0.005-5% w/w, or 0.01-10% w/w, of medicament relative to the total weight of the formulati on. 100781 In solid dosage forms for oral administration (capsules, tablets, pills, dragees, powders, granules and the like), the subject composition is mixed with one or more pharmaceutically acceptable carriers and/or any of the following: (1) fillers or extenders, such as starches, lactose, sucrose, glucose, mannitol, and/or silicic acid; (2) binders, such as, for example, carboxymethyl cellulose, alginates, gelatin, polyvinyl pyrrolidone, sucrose and/or acacia; (3) humectants, such as glycerol; (4) disintegrating agents, such as agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate; (5) solution retarding agents, such as paraffin; (6) absorption accelerators, such as quaternary ammonium compounds; (7) wetting agents, such as, for example, acetyl alcohol and glycerol monostearate; (8) absorbents, such as kaolin and bentonite clay; (9) lubricants, such a tale, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof; and (10) coloring agents. In the case of capsules, tablets and pills, the pharmaceutical compositions may also comprise buffering agents- Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using lactose or milk sugars, as well as high molecular weight polyethylene glycols and the like. 100791 Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions. syrups and elixirs. In addition to the subject compositions, the liquid dosage forms may contain inert diluents commonly used in the art, such as, for example, water or other solvents, solubilizing agents and emulsifiers, such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, oils (in particular, cottonseed, corn, peanut, sunflower, soybean, olive, castor, and sesame oils), glycerol, tetrahydrofuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof WO 2013/167996 PCT/IB2013/050904 29 0080 Suspensions, in addition to the subject compositions, may contain suspending agents such as, for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol, and sorbitan esters, microcrystalline cellulose, aluminum inetahydroxide, bentonite. agar agar and tragacanth, and mixtures thereof, [00811 Formulations for rectal or vaginal administration may be presented as a suppository, which may be prepared by mixing a SLbject composition with one or more suitable non-irritating carriers comprising, for example, cocoa butter, polyethylene glycol, a suppository wax. or a salicylate, and which is solid at room temperature, but liquid at body temperature and, therefore, will, melt in the appropriate body cavity and release the encapsulated compound(s) and composition(s). Formulations which are suitable for vaginal administration also include pessaries, tampons, creams, gels, pastes, foams, or spray formulations containing such carriers as are known in the art to be appropriate. 100821 Dosage forms for transdermal administration include powders, sprays, ointments, pastes, creams, lotions, gels, solutions, patches, and inhalants. A subject composition may be mixed under sterile conditions with a pharmaceutically acceptable carrier, and with any preservatives, buffers, or propellants that may be required. For transdermal administration, the complexes may include lipophilic and hydrophilic groups to achieve the desired water solubility and transport properties. [00831 The ointments, pastes, creams and gels may contain, in addition to subject compositions, other carriers, such as animal and vegetable fats, oils, waxes, paraffins, starch, tragacanth, cellulose derivatves, polyethylene glycols, silicones, bentonites, silicic acid, talc and zinc oxide, or mixtures thereof Powders and sprays may contain, in addition to a subject composition, recipients such as lactose, talc, silicic acid, aluminum hydroxide, calcium silicates and polyam ide powder, or mixtures of such substances. Sprays may addi tionally contain customary propellants, such as chl orofiluoroh ydrocarbons and volatile unsubstituted hydrocarbons, such as butane and propane.

WO 2013/167996 PCT/IB2013/050904 30 100841 Methods of delivering a composition or compositions via a transdermal patch are known in the art Exemplary patches and methods of patch delivery are described in US Patent Nos. 6,974,588, 6,564,093, 6,312,716, 6,440,454, 6,267,983, 6,239,180, and 6,103,275. 100851 In another embodiment, a transdermal patch may comprise: a substrate sheet comprising a composite film formed of a resin composition comprising 100 parts by weight of a polyvinyl chloride-polyurethane composite and 2-10 pans by weight of a styrene-ethylene-butyiene-styrene copolyner, a first adhesive layer on the one side of the composite film, and a polyalkylene terephthalate film adhered to the one side of the composite film by means of the first adhesive layer, a primer layer which comprises a saturated polyester resin and is formed on the surface of the polyalkylene terephthalate film; and a second adhesive layer comprising a styrene-diene styrene block copolymer containing a pharmaceutical agent layered on the primer layer. A method for the manufacture of the above-mentioned substrate sheet comprises preparing the above resin composition molding the resin composition into a composite film by a calendar process, and then adhering a polyakylene terephthalate film on one side of the composite film by means of an adhesive layer thereby forming the substrate sheet, and forming a primer layer composing a saturated polyester resin on the outer surface of the polyalkylene terephthalate film. [00861 Another type of patch comprises incorporating the drug directly in a pharmaceutically acceptable adhesive and laminating the drug-containing adhesive onto a suitable backing member, e.g. a polyester backing membrane. The drug should be present at a concentration which will not affect the adhesive properties, and at the same time deliver the required clinical dose. [0087J Transdermal patches may be passive or active. Passive transdermal drug delivery systems currently available, such as the nicotine, estrogen and nitroglycerine patches, deliver small-molecule drugs. Many of the newly developed proteins and peptide WO 2013/167996 PCT/IB2013/050904 31 drugs are too large to be delivered through passive transdermial patches and may be delivered using technology such as electrical assist (iontophoresis) for large-molecule diugs. 100881 tontophoresis is a technique employed for enhancing the flux of ionized substances through membranes by application of electric current One example of an iontophoretic membrane is given in U.S. Pat. No. 5,080,646 to Theeuwes. The principal mechanisms by which iontophoresis enhances molecular transport across the skin are (a) repelling a charged ion from an electrode of the same charge, (b) electroosmosis, the convective movement of solvent that occurs through a charged pore in response the preferential passage of counter-ions when an electric field is applied or (c) increase skin permeability due to application of electrical current [00891 In some cases, it may be desirable to administer in the form of a kit, it may comprise it container for containing the separate compositions such as a divided bottle or a divided foil packet. Typically the kit comprises directions for the administration of the separate components. The kit form is particularly advantageous when the separate components are preferably administered in different dosage forms (e.g., oral and parenteral), are administered at different dosage intervals, or when titration of the Individual components of the combination is desired by the prescribing physician. 100901 An example of such a kit is a so-called blister pack. Blister packs are well known in the packaging industry and are widely used for the packaging of pharmaceutical unit dosage forms (tablets, capsules, and the like). Blister packs generally consist of a sheet of relatively stiff material covered with a foil of a plastic material that may be transparent, 100911 Methods and compositions for the treatment of metabolic syndrome. Among other things, herein is provided a method of treating metabolic syndrome, comprising administering to a patient tin need thereof a therapeutically effective amount of compound of Formula 1: WO 2013/167996 PCT/IB2013/050904 32 0 R CF , R1 Formula I Wher-einl, R independently represents halogen ~F, [1 ~Cl, -D or -Br, H O 'N R independently represents -- H11 -D, or R independently represents Ho, D , 0 O

R

2 o in epn enl repesnt 0i 0D o 0 0 0 /1 WO 2013/167996 PCT/IB2013/050904 33 0 0 N0 NH2 NH2 , H H NN Nj HH H H CIN N O OH H H HH R4 independently represents WO 2013/167996 PCT/IB2013/050904 34

NHCOCH

3 OH CO2H CCONH 2 OHO OH 0 H 2 N OH O0 0 0 HO s NH2 O O 2 WO 2013/167996 PCT/IB2013/050904 35 0 12 00 N 7 NH NH N\ HN N N N H NN N /H H N H 0 HN) NH H H H H

O

WO 2013/167996 PCT/IB2013/050904 36 OH OH HO 0HO0 HS

NH

2 HO NH 0 O0 N~ NH N N H 0 00 0 OH OO 01 0 b a is independently 2,3 or 7; each b is independently 3, 5 or 6; e is independently 1, 2 or 6; c and d are each independently H, D, -OH, -OD, C 1 -Q-alkyl, -N:H: or -COCH Alethoth for using compounds /fr frua I: 100921 The invention also includes methods for treating metabolic syndrome, hyperuricemia, gout, dyslipidemia, obesity, urea cycle disorders, hyperglycemia, insuli.n resistance, diabetes mellitus, diabetes insipidus, type I diabetes, type 2 diabetes, microvascular complications, macrovascular complications, lipid disorders, prediabetes, WO 2013/167996 PCT/IB2013/050904 37 obesity, arrhythmia, myocardial infarction, stroke, neuropathy, renal complications, hypertriglyceridemia, cardiovascular complications, and post prandial hyperglycemia METHODS OF MAKING 100931 Examples of synthetic pathways usef l for making compounds of formula I are set forth in example below and generalized in scheme I Scheme-I: HO C H H3- HO 1 2 3 COC Br Br HO 704 C 2h C85 "C 1 a [ I BrCF CE-. uOLU ........ ... 0 *C-Hl, 1) - TH OH 5 C0 14 CF Oft> UOH, H^O, TH F H 20O 0-4 'C Ih C C 10 -HO ~ Ottx horide CH C1 1 rt, 2h CE> OH 1000C 12

CC

WO 2013/167996 PCT/IB2013/050904 38 100941 Step-I: Synthesis of compound 3: HO" 0 H MNO 0 "Ct HO 0 + 0\ 3 days N 2 3 [00951 Pyrrolidine 2 (120 g, 1.69 mol; 2 eq.) was added dropwise to 100 g (0.847 mol) of ethyl (S)-(-)-lactate 1 at OC and stirred at room temperature for 3 days. After removal of excess pyrrolidine and resulting ethanol in vacuo, the oil residue was purified with distillation (104 "C, 2 mmtlg) to give 113 g (93%) of compound 3 as pale- yellow oil. 100961 Step-2: Synthesis of compound 7: SOC7 COOH C1CH 2 CH l Br B0Co B+ HB 70 0 C. 2h C 85 0 C,18h N CE 5ACOM6 4 a 6 Br Et-'o NA 0 0 Cr1h a 100971 To a 24 3 -neck flask under air, immersed in an oil bath and fitted with an addition funnel and a condenser was added 500 mL., of anhydrous 1,2-dichloroethane, 4 chlorophenylacetic acid 4 (174.04 g 98%, 1.0 mol (Acros)) of in one-portion, DMF (0.40 mL, ca. 0.5 mol%) in one-portion and thionyl chloride (95 mL, 1.3 mol, 1 .3 eq.) over - I minute. The resulting mixture was heated to 70 (C (oil-bath temperature) over 15 minutes. Vigorous gas evolution began approximately 5 minutes after heating (at ~40 45 0C). The vigorous gas evolution slowed to a steady stream and then the gas evolution stopped. After stirring at 70 ) for 2 hours, bromine (80 mL, ca, 249 g, 1,55 mol; 1 .55 eq.) was added to the resulting pale yellow solution (at 65 0 C) over ~1 minute to give a brown solution, The reaction was stirred at 80 0 C to 85 C (oil-bath temperature) for 18 h and then cooled to room temperature. This u-bromo acid chloride solution 6 was stored at room temperature and used in the next ester formation step without further purification.

WO 2013/167996 PCT/IB2013/050904 39 100981 The solution of crude a-bromo acid chloride 6 (138 g, ~ 0.138 mol) in 1,2 dichloroethane prepared above was diluted with 100 mL of 1,2-dichloroethane. Excess bromine was removed by distillation in vacuo until ca, 100 mL of solution remained, The acid chloride solution was then added dropwise to a solution of compound 3 (20,1 g, 0.140 mol) and triethylamine (14.78 g, 0.147 mol) in 100 mL of 1,2-dichloroethane at 0 tC. The resulting brown mixture was warmed up to room temperature over 1 h. The reaction mixture was quenched with water (100 mL) and the organic layer was separated and washed with 100 mL of 10% Na 2 S203 and then with saturated NaHCO (100 mL). The organic layer was dried over Na2SO 4 and then concentrated in vacuo to give 45.8 g of crude product 7 as brown oil which was used in the next step without further purification. [00991 Step-3: Synthesis of compound 10: Br c Fs cF 3 +S LC H H 0F HH 25 1 oH UGH, HWa, THF0 0-4 OC, I hn J 10 [00100] To a solution of aAa-trifluoro-m-cresol 8 (3.3 g, 0.0204 mol) in anhydrous THF (20 mL) at room temperature was added dropwise lithium tert-butoxide (20 mL of a 1.0 M solution in THF; 0.02 mol). The resulting lithium phenoxide solution was added dropwise to a solution of bromide 7 (crude, 7.5 g; 0.02 mol) in 40 niL of THF at -5 "C After stirring at -5 )C for 1 hour, a pre-mixed solution of hydrogen peroxide (Fisher 30%; 105 mL, 0.4 mol) and LiOHIH 2 0 (21g, 0.05 mol) in water (50 mL) was added at room temperature over 20 mir. The reaction was stirred at 0-4 C for I hour, quenched with saturated aqueous sodium bisulfite (150 mL), then IN HCl was added to adjust the pH of the solution to about 2. THF was removed by distillation in vacuo, and then the reaction WO 2013/167996 PCT/IB2013/050904 40 mixture was diluted with EtOAc (100 mL). The organic layer was washed with water and brine, dried over Na 2

SO

4 and evaporated to give 7g of crude acid 10. The crude acid was crystallized from heptane to give 4.6 g of a white solid. Chiral HPLC analysis 96.5:3.5 enantiomiers, 1001011 Step-4: Synthesis of compound 12 0 HO Oxaloyl chloride CHOI 11 rt, 2h 0 c1 -1 12 1001021 To a solution of 605 mg of 5,8, 11,1417-eicosapentaenic acid 1I in 6 ml of dry chloroform was added 0 25 ml of oxalyl chloride under argon at room temperature. The mixture was reacted for 2 hours. From the reaction mixture were removed the chloroform and the remaining oxalyl chloride by distillation under reduced pressure to give 5,8, 11,14,17-eicosapentaenoyl chloride 12, which was then directly used for the next step. 1001031 Step-5: Synthesis of compound 13: cF 3 ioc H 100'C 12 "I e C 10 -t, 0, 0 1c0 13 WO 2013/167996 PCT/IB2013/050904 41 1001041 A solution of compound 10 (2 mmol) in dry DIMF (10 ml) was suspended in anhydrous potassium carbonate (2.2 mmol) at room temperature. To the reaction mixture was added the DMF solution 2 mmol of 12 in 2 ml DMF prepared above dropwise over 15 min- and the mixture was heated at 100 *C for S hours, From the reaction mixture was removed insolubles by filtration, and water was added to the filtrate. The mixture was extracted two times with ethyl acetate (2x 10 ml). Organic layer of the extract was washed with water and dried over anhydrous sodium sulfate. Then, the solvent was removed by distillation under reduced pressure to get the residue was subjected to silica gel column chromatography to obtain the pure compound 13. [00.1051 The term "sample" refers to a sample of a body -fluid, to a sample of separated cells or to a sample from a tissue or an organ. Samples of body fluids can be obtained by well known techniques and include, preferably, samples of blood, plasma, serum, or urine, more preferably, samples of blood, plasma or serum. EQUIVALENTS 100106 The present disclosure provides among other things compositions and methods for treating metabolic syndrome and their complications. While specific embodiments of the subject disclosure have been discussed, the above specification is illustrative and not restrictive. Many variations of the systems and methods herein will become apparent to those skilled in the art upon review of this specification. The full scope of the claimed systems and methods should be determined by reference to the claims, along with their full scope of equivalents, and the specification, along with such variations. INCORPORATION BY REFERENCE [001071 All publications and patents mentioned herein, including those items listed above, are hereby incorporated by reference in. their entirety as if each individual publication or patent was specifically and individually indicated to be incorporated by reference. In case of conflict, the present application, including any definitions herein. wIll control

Claims (7)

1. 2. A Pharmaceutical composition comprising a compound of claim 1 and a pharmaceutical acceptable carrier.
2. 3. The pharmaceutical composition of claiin 2, which is fornulated to treat the underlying etiology with an effective amount administering the patient in need by oral administration, delayed release or sustained release, transmucosal, syrup, WO 2013/167996 PCT/IB2013/050904 47 topical, parenteral administration, injection, subdermal, oral solution, rectal adminisation, buccal administration or transdermal administration.
3. 4. Compounds and compositions of claim 3 are formulated for the treatment of metabolic syndrome, hyperuricemia, gout, dyslipidemia, obesity, urea cycle disorders, hyperglycermia, insulin resistance, diabetes mellitus, diabetes insipidus, type I diabetes, type 2 diabetes, microvascular complications, m acrovascular complications, lipid disorders, prediabetes, obesity, arrhythmia, myocardial infarction., stroke, neuropathy, renal complications, hypertriglyceridemia, cardiovascular complications, and post prandial hyperglycemia. 5 A molecular conjugate of arhalofenate and R-Lipoic acid.
4. 6. A molecular conjugate of arhalofenate and eicosapentaenoic acid.
5. 7. A molecular conjugate of arhalofenate and docosahexaenoic acid.
6. 8. A molecular conIugate of arhalofenate and acetyl cysteine.
7. 9. A molecular conjugate of arhalofenate and salsalate.