AU2013202036C1 - Anti-GCC antibody molecules and related compositions and methods - Google Patents
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Abstract
Antibodies and antigen-binding fragments of antibodies that bind GCC are disclosed. The antibodies bind an extracellular domain of GCC and can be internalized. In some embodiments, the antibodies are humanized, chimeric or human. Nucleic acids and vectors encoding the antibodies or portions thereof, recombinant cells that contain the nucleic acids, and compositions comprising the antibodies or antigen-binding fragments are also disclosed. The invention also provides therapeutic and diagnostic methods utilizing the antibodies and antigen-binding fragments provided herein. 210b
Description
ANTI-GCC ANTIBODY MOLECULES AND RELATED COMPOSITIONS AND METHODS This application is a divisional of Australian Application No. 2010310545, the entire contents of which are incorporated herein by reference. RELATED APPLICATIONS [001] The present application claims the benefit of U.S. Provisional Application Serial No. 61/254,474, filed October 23, 2009. The entire content of U.S. Provisional Application Serial No. 61/254,474 is incorporated herein by this reference. FIELD OF INVENTION [0021 The invention relates to antibody molecules which bind GCC, as well as to related molecules, e.g., nucleic acids which encode such antibody molecules, compositions, and related methods, e.g., therapeutic and diagnostic methods. BACKGROUND [003] Guanylyl cyclase C (GCC) is a transmembrane cell surface receptor that functions in the maintenance of intestinal fluid, electrolyte homeostasis and cell proliferation, see, e.g., Carrithers et al., Proc. Natd. Acad. Sci. USA 100:3018-3020 (2003). GCC is expressed at the mucosal cells lining the small intestine, large intestine and rectum (Carrithers et al., Dis Colon Rectum 39: 171-181 (1996)). GCC expression is maintained upon neoplastic transformation of intestinal epithelial cells, with expression in all primary and metastatic colorectal tumors (Carrithers et al., Dis Colon Rectum 39:171-181 (1996); Buc et al. Eur J Cancer 41: 1618-1627 (2005); Carrithers et al., Gastroenterology 107: 1653-1661(1994)).
SUMMARY [0041 The inventors have discovered numerous anti-GCC antibodies, including both human and murine antibodies. Accordingly, in one aspect, the invention features an anti-GCC antibody molecule, as disclosed herein. The anti-GCC antibody molecules are useful as naked antibody molecules and as components of immunoconjugates. Accordingly, in another aspect, the invention features immunoconjugates comprising an anti-GCC antibody molecule and a therapeutic agent or label. The invention also features pharmaceutical compositions comprising the anti-GCC antibody molecules and immunoconjugates described herein. The invention also features methods of using the anti-GCC antibody molecules and immunoconjugates described herein for detection of GCC and of cells or tissues that express GCC; for diagnosis, prognosis, imaging, or staging of a GCC-mediated disease; for modulating an activity or function of a GCC protein; and for treatment of a GCC-mediated disease, as described herein. In another aspect, the invention also features isolated and/or recombinant nucleic acids encoding ant GCC antibody molecule amino acid sequences, as well as vectors and host cells comprising such nucleic acids, and methods for producing anti-GCC antibody molecules, [0051 All publications, patent applications, patents and other references mentioned herein are incorporated by references in their entirety, [0061 Other features, objects, and advantages of the invention(s) disclosed herein will be apparent from the description and drawings, and from the claims. DESCRIPTION OF THE FIGURES [007) Figure 1 depicts tumor growth in 293-GCC#2 bearing SCID mice treated with 5F9vc-MMAF, -DMI, and -DM4 on a qi4d schedule [008} Figure 2 depicts the tumor growth of mice treated with 0,9%NaC1: 209 antibody at 40mg/kg; or 5F9 antibody at 10 or 40mg/kg. [009 Figure 3 depicts lung weight of mice treated with 0.%ONaCi; 209 antibody at 40mg/kg; or 5F9 antibody at 10 or 40mg/k;g on day 41 pd. [010] Figure 4 depicts the survival curve of CII26-hGCC tunor-bearing mice treated wit SF9 antibody.
WO 2011/050242 PCT/UJS2010/053686 DETAILED DESCRIPTION GuanYlyl Cyclase C [0111 Guanylyl cyclase C (GCC) (also known as STAR, ST Receptor, GUC2C, and GUCY2C) is a transmembrane cell surface receptor that functions in the maintenance of intestinal fluid, electrolyte homeostasis and cell proliferation (Carrithers et al., Proc Nad Acad Sci US A 100: 3018-3020 (2003); Mann et al., Biochem Biophys Res Commun 239: 463-466 (1997); Pitari et al., Proc Natl AcadSci USA 100: 2695-2699 (2003)); GenBank Accession No. NM_004963, each of which is incorporated herein by reference). This function is mediated through binding of guanylin (Wiegand et al. FEBS Lett. 311:150-154 (1992)). GCC also is a receptor for heat-stable enterotoxin (ST, e.g., having an amino acid sequence of NTFYCCELCCNPACAGCY, SEQ ID NO:316) which is a peptide produced by E. coli, as well as other infectious organisms (Rao, M.C. Ciba Found. Symp. 112:74-93 (1985); Knoop F.C. and Owens, M. J. Pharmacol. ToxicoL Methods 28:67-72 (1992)). Binding of ST to GCC activates a signal cascade that results in enteric disease, e.g., diarrhea. [0121 Nucleotide sequence for human GCC (GenBank Accession No. NM_004963): 1 atgaagacgt tgctgttgga cttggetttg tggtcactge tettecagcc cgggtggctg 61 teetttagtt cccaggtgag tcagaactgc cacaatggca getatgaaat cagcgtcctg 121 atgatgggca acteagcett tgcagagccc ctgaaaaact tggaagatge ggtgaatgag 181 gggctggaaa tagtgagagg acgtctgcaa aatgctggcc taaatgtgac tgtgaacget 241 actttcatgt attcggatgg tctgattcat aactcaggcg actgceggag tagcacctgt 301 gaaggecteg acctactcag gaaaatttca aatgcacaac ggatgggctg tgtectcata 361 gggecctcat gtacatactc caccttccag atgtaccttg acacagaatt gagetacccc 421 atgatetcag ctggaagttt tggattgtca tgtgactata aagaaacett aaccaggctg 481 atgtctccag ctagaaagtt gatgtactte ttggttaact tttggaaaac caacgatctg 541 ccettcaaaa ettattcctg gagcacttcg tatgtttaca agaatggtac agaaactgag 601 gactgtttct ggtaccttaa tgetctggag gctagcgttt cetatttctc ccacgaactc 661 ggctttaagg tggtgttaag acaagataag gagtttcagg atatettaat ggaccacaac 3 WO 2011/050242 PCT/US2010/053686 721 aggaaaagca atgtgattat tatgtgtggt ggtccagagt tctctacaa gctgaagggt 781 gaccgagcag tggctgaaga cattgtcatt attctagtgg atcttttcaa tgaccagtac 841 tttgaggaca atgtcacagc ccctgactat atgaaaaatg tccttgttet gacgctgtct 901 cctgggaatt ccettctaaa tagctcttto tccaggaatc tatcaccaac aaaacgagac 961 tttgctcttg cctatttgaa tggaatcctg ctctttggac atatgctgaa gatatttctt 1021 gaaaatggag aaaatattac cacccccaaa tttgctcatg ctttcaggaa tctcactttt 1081 gaagggtatg acggtccagt gaccttggat gactgggggg atgttgacag taccatggtg 1141 cttctgtata cctctgtgga caccaagaaa tacaaggttc ttttgaocta tgatacccac 1201 gtaaataaga cctatectgt ggatatgagc cccacattca cttggaagaa ctctaaactt 1261 cctaatgata ttacaggccg gggccetcag atectgatga ttgcagtett caccctcact 1321 ggagctgtgg tgotgetect gotcgtcgct ctcctgatgc tcagaaaata tagaaaagat 1381 tatgaacttc gtcagaaaaa atggtcccac attcctcctg aaaatatett toctctggag 1441 accaatgaga ccaatcatgt tagcctcaag atcgatgatg acaaaagacg agatacaatc 1501 cagagactac gacagtgcaa atacgacaaa aagcgagtga ttctcaaaga tctcaagcac 1561 aatgatggta atttcactga aaaacagaag atagaattga acaagttgct tcagattgac 1621 tattacaacc tgaccaagtt ctacggcaca gtgaaacttg ataccatgat ctteggggtg 1681 atagaatact gtgagagagg atccctcegg gaagttttaa atgacacaat ttcctaccct 1741 gatggcacat tcatggattg ggagtttaag atctctgtet tgtatgacat tgctaaggga 1801 atgtcatatc tgcactccag taagacagaa gtccatggtc gtctgaaatc taccaactgc 1861 gtagtggaca gtagaatggt ggtgaagatc actgattttg gctgcaattc cattttacct 1921 ccaaaaaagg acctgtggac agctccagag cacctccgcc aagccaacat ctctcagaaa 1981 ggagatgtgt acagctatgg gatcatcgca caggagatca tcctgcggaa agaaaccttc 2041 tacactttga gotgtcggga ccggaatgag aagattttca gagtggaaaa ttccaatgga 2101 atgaaaccct tecgeccaga tttattcttg gaaacagcag aggaaaaaga gctagaagtg 2161 tacctacttg taaaaaactg ttgggaggaa gatccagaaa agagaccaga tttcaaaaaa 2221 attgagacta cacttgccaa gatatttgga ctttttcatg accaaaaaaa tgaaagctat 4 WO 2011/050242 PCT/US2010/053686 2281 atggatacct tgatccgacg totacageta tattctcgaa acctggaaca tctggtagag 2341 gaaaggacac agctgtacaa ggcagagagg gacagggctg acagacttaa ctttatgttg 2401 cttccaaggc tagtggtaaa gtctctgaag gagaaagget ttgtggagcc ggaactatat 2461 gaggaagtta caatctactt cagtgacatt gtaggtttca ctactatctg caaatacagc 2521 acccccatgg aagtggtgga catgcttaat gacatetata agagttttga ccacattgtt 2581 gatcatcatg atgtotacaa ggtggaaacc atcggtgatg cgtacatggt ggctagtggt 2641 ttgcctaaga gaaatggcaa tcggcatgca atagacattg ccaagatggc cttggaaatc 2701 ctcagcttca tggggacett tgagctggag catcttcctg gcctcccaat atggattcgc 2761 attggagttc actctggtcc etgtgctgct ggagttgtgg gaatcaagat gcctogttat 2821 tgtctatttg gagatacggt caacacagcc tctaggatgg aatccactgg cctccctttg 2881 agaattcacg tgagtggctc caccatagcc atcctgaaga gaactgagtg ccagttcctt 2941 tatgaagtga gaggagaaac atacttaaag ggaagaggaa atgagactac ctactggctg 3001 actgggatga aggaccagaa attcaacctg ccaaccccte ctactgtgga gaatcaacag 3061 cgtttgcaag cagaattttc agacatgatt gccaactctt tacagaaaag acaggcagca 3121 gggataagaa gccaaaaacc cagacgggta gccagetata aaaaaggcac tctggaatac 3181 ttgcagctga ataccacaga caaggagagc acctattttt aa (SEQ ID NO:227) [013] Amino acid sequence for human GCC (GenPept Accession No. NP_004954): 1 mktllidlal wsllfqpgwl sfssqvsqnc hngsyeisvl mmgnsafaep lknledavne 61 gleivrgrlq naglnvtvna tfinysdglih nsgdcrsstc egldllrkis naqrmgcvli 121 gpsctystfq myldtelsyp misagsfgls cdyketltri msparktmyf lvnfwktndl 181 pfktyswsts yvykngtete dcfwylnale asvsyfshel gfkvvlrqdk efqdilmdhn 241 rksnviimcg gpeflyklkg dravaedivi ilvdlfidqy fednvtapdy mknvlvltls 301 pgnsllnssf srnlsptkrd falaylngil lfghmlkifl engenittpk fahafrnltf 5 WO 2011/050242 PCT/US2010/053686 361 egydgpvtld dwgdvdstmv llytsvdtkk ykvlltydth vnktypvdms ptftwknskl 421 pnditgrgpq ilmiavftlt gavvllllva llmlrkyrkd yelrqkkwsh ippenifple 481 tnetnhvsk idddkrrdti qrlrqckydk krvilkdlkh ndgnftekqk iekildlqid 541 yyntkfygt vkldtmifgv ieycergslr evlndtisyp dgtfndwefk isvlydiakg 601 msylhsskte vhgrlkstnc vvdsrmvvki tdfgonsilp pkkdlwtape hlrqanisqk 661 gdvysygiia qeiilrketf ytlscrdme kifrvensng mkpfrpdlfl etaeekelev 721 ylivkncwee dpekrpdfkk iettlakifg lfhdqknesy mdtlirrlql ysrnlehlve 781 ertqlykaer dradrlnfml lprlvvkslk ekgfvepely eevtiyfsdi vgfttickys 841 tpmevvdmln diyksfdhiv dhhdvykvet igdaymvasg lpkrngnrha idiakmalei 901 lsfingtfele hlpglpiwir igvhsgpcaa gvvgikmpry clfgdtvnta srmestglpl 961 rihvsgstia ilkrtecqfl yevrgetylk grgnettywl tgmkdqkfnl ptpptvenqq 1021 rlqaefsdmi anslqkrqaa girsqkprrv asykkgtley lqlnttdkes tyf (SEQ ID NO:228) [014] The GCC protein has some generally accepted domains each of which contributes a separable function to the GCC molecule. The portions of GCC include a signal sequence (for directing the protein to the cell surface) from amino acid residue 1 to about residue 23, or residue I to about residue 21 of SEQ ID NO:228 (excised for maturation to yield functional mature protein from about amino acid residues 22 or 24 to 1073 of SEQ ID NO:228), an extracellular domain for ligand, e.g., guanylin or ST, binding from about amino acid residue 24 to about residue 420, or about residue 54 to about residue 384 of SEQ ID NO:228, a transmembrane domain from about amino acid residue 431 to about residue 454, or about residue 436 to about residue 452 of SEQ ID NO:228, a kinase homology domain, predicted to have tyrosine kinase activity from about amino acid residue 489 to about residue 749, or about residue 508 to about residue 745 of SEQ ID NO:228 and a guanylyl cyclase catalytic domain from about residue 750 to about residue 1007, or about residue 816 to about residue 1002 of SEQ ID NO:228. [015] In normal human tissues, GCC is expressed at the mucosal cells, e.g., at the apical brush border membranes, lining the small intestine, large intestine and rectum 6 WO 2011/050242 PCT/US201i1053686 (Carrithers et al., Dis Colon Rectum 39: 171-181 (1996)). GCC expression is maintained upon neoplastic transformation of intestinal epithelial cells, with expression in all primary and metastatic colorectal tumors (Carrithers et al., Dis Colon Rectum 39: 171-181 (1996); Buc et alt Eur JCancer 41: 1618-1627 (2005); Carrithers et al., Gastroenterology 107: 1653-1661(1994)). Neoplastic cells from the stomach, esophagus and the gastroesophageal junction also express GCC (see, e.g., U.S. Patent No. 6,767,704; Debruyne et al. Gastroenterology 130:1191-1206 (2006)). The tissue-specific expression and association with cancer, e.g., of gastrointestinal origin, (e.g., colon cancer, stomach cancer, or esophageal cancer), can be exploited for the use of GCC as a diagnostic marker for this disease (Carrithers et al., Dis Colon Rectum 39: 171-181 (1996); Buc et al. Eur J Cancer 41: 1618-1627 (2005)). [0161 As a cell surface protein, GCC can also serve as a therapeutic target for receptor binding proteins such as antibodies or ligands. In normal intestinal tissue, GCC is expressed on the apical side of epithelial cell tight junctions that form an impermeable barrier between the luminal environment and vascular compartment (Almenoff et al., Mol Microbiol 8: 865-873); Guarino et al., Dig Dis Sci 32: 1017-1026 (1987)). As such, systemic intravenous administration of a GCC-binding protein therapeutic will have minimal effect on intestinal GCC receptors, while having access to neoplastic cells of the gastrointestinal system, including invasive or metastatic colon cancer cells, extraintestinal or metastatic colon tumors, esophageal tumors or stomach tumors, adenocarcinoma at the gastroesophageal junction. Additionally, GCC internalizes through receptor mediated endocytosis upon ligand binding (Buc et al. Eur JCancer 41: 1618-1627 (2005); Urbanski et al., Biochem Biophys Acta 1245: 29-36 (1995)). [017] Polyclonal antibodies raised against the extracellular domain of GCC (Nandi et al. Protein Expr. Purif 8:151-159 (1996)) were able to inhibit the ST peptide binding to human and rat GCC and inhibit ST-mediated cGMP production by human GCC. [0181 GCC has been characterized as a protein involved in cancers, including colon cancers. See also, Carrithers et al., Dis Colon Rectum 39: 171-181 (1996); Buc et al. Eur JCancer 41: 1618-1627 (2005); Carrithers et al,, Gastroenterology 107: 1653 1661(1994); Urbanski et al., Biochem Biophys Acta 1245: 29-36 (1995). Antibody molecule therapeutics directed to GCC can be used alone in unconjugated form to thereby 7 WO 2011/050242 PCT/US2o1o/053686 inhibit the GCC-expressing cancerous cells. Anti-GCC antibody molecules of the invention can bind human GCC. In some embodiments, an anti-GCC antibody molecule of the invention can inhibit the binding of a ligand, e.g., guanylin or heat-stable enterotoxin to GCC. In other embodiments, an anti-GCC antibody molecule of the invention does not inhibit the binding of a ligand, e.g., guanylin or heat-stable enterotoxin to GCC. [019] Monoclonal antibodies specific for GCC include GCC:B 10 (Nandi et at., J. Cell. Biochem. 66:500-511 (1997)), GCC:4D7 (Vijayachandra et al. Biochemistry 39:16075-16083 (2000)) and GCC:C8 (Bakre et al. Eur. J. Biochem. 267:179-187 (2000)). GCC:B1O has a kappa light chain and an IgG2a isotype and cross-reacts to rat, pig and monkey GCC. GCC :B 10 binds to the first 63 amino acids of the intracellular domain of GCC, specifically to residues 470-480 of SEQ ID NO:228 (Nandi et al. Protein Sci. 7:2175-2183 (1998)). GCC:4D7 binds to the kinase homology domain, within residues 491-568 of GCC (Bhandari et al. Biochemistry 40:9196-9206 (2001)). GCC:C8 binds to the protein kinase-like domain in the cytoplasmic portion of GCC. Definitions and Methods [020] Unless otherwise defined herein, scientific and technical terms used in connection with the present invention have the meanings that are commonly understood by those of ordinary skill in the art. Generally, nomenclature utilized in connection with, and techniques of, cell and tissue culture, molecular biology, and protein and oligo- or polynucleotide chemistry and hybridization described herein are those known in the art. GenBank or GenPept accession numbers and useful nucleic acid and peptide sequences can be found at the website maintained by the National Center fbr Biotechnological Information, Bethesda MD. Standard techniques are used for recombinant DNA, oligonucleotide synthesis, and tissue culture and transformation and transfection (e.g., electroporation, lipofection). Enzymatic reactions and purification techniques are performed according to manufacturer's specifications or as commonly accomplished in the art or as described herein. The foregoing techniques and procedures are generally performed according to methods known in the art, e.g., as described in various general and more specific references that are cited and discussed throughout the present specification. See e.g., Sambrook et al. Molecular Cloning: A Laboratory Manual (3rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (2000)) or see generally, Harlow, E. 8 WO 2011/050242 PCT/US2010/053686 and Lane, D. (1988) Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. The nomenclatures utilized in connection with, and the laboratory procedures and techniques of, analytical chemistry, synthetic organic chemistry, and medicinal and pharmaceutical chemistry described herein are known in the art. Standard techniques are used for chemical syntheses, chemical analyses, pharmaceutical preparation, formulation, and delivery, and treatment of patients. Furthermore, unless otherwise required by context, singular terms shall include pluralities and plural terms shall include the singular. [021] As used herein, the term "antibody molecule" refers to an antibody, antibody peptide(s) or immunoglobulin, or an antigen binding fragment of any of the foregoing, e.g., of an antibody. Antibody molecules include single chain antibody molecules, e.g., scFv, see. e.g., Bird et al. (1988) Science 242:423426; and Huston et al. (1988) Proc. Natl. Acad. Sci. USA 85:5879-5883), and single domain antibody molecules, see, e.g., W09404678. Although not within the term "antibody molecules," the invention also includes "antibody analog(s)," other non-antibody molecule protein-based scaffolds, e.g., fusion proteins and/or immunoconjugates that use CDRs to provide specific antigen binding. [0221 An "anti-GCC antibody molecule" refers to an antibody molecule (i.e., an antibody, antigen-binding fragment of an antibody or antibody analog) which interacts with or recognizes, e.g., binds (e.g., binds specifically) to GCC, e.g., human GCC. Exemplary anti-GCC antibody molecules are such as those summarized in Tables 1 and 2. [0231 As used herein, the term "antibody," "antibody peptide(s)" or "immunoglobulin" refers to single chain, two-chain, and multi-chain proteins and glycoproteins. The term antibody includes polyclonal, monoclonal, chimeric, CDR grafted and human or humanized antibodies, all of which are discussed in more detail elsewhere herein. Also included within the term are camelid antibodies, see, e.g., US2005/0037421, and nanobodies, e.g., IgNARs (shark antibodies), see, e.g., W003/014161. The term "antibody" also includes synthetic and genetically engineered variants. [024] As used herein, the term "antibody fragment" or "antigen binding fragment" of an antibody refers, e.g., to Fab, Fab', F(ab') 2 , and Fv fragments, single chain antibodies, functional heavy chain antibodies (nanobodies), as well as any portion of an 9 WO 2011/050242 PCT/US2010053686 antibody having specificity toward at least one desired epitope, that competes with the intact antibody for specific binding (e.g., a fragment having sufficient CDR sequences and having sufficient framework sequences so as to bind specifically to an epitope). E.g., an antigen binding fragment can compete for binding to an epitope which binds the antibody from which the fragment was derived. Derived, as used in this and similar contexts, does not imply any particular method or process of derivation, but can refer merely to sequence similarity. Antigen binding fragments can be produced by recombinant techniques, or by enzymatic or chemical cleavage of an intact antibody. The term, antigen binding fragment, when used with a single chain, e.g., a heavy chain, of an antibody having a light and heavy chain means that the fragment of the chain is sufficient such that when paired with a complete variable region of the other chain, e.g., the light chain, it will allow binding of at least 25, 50, 75, 85 or 90% of that seen with the whole heavy and light variable region.) [025] The term, "antigen binding constellation of CDRs" or "a number of CDRs sufficient to allow binding" (and similar language), as used herein, refers to sufficient CDRs of a chain, e.g., the heavy chain, such that when placed in a framework and paired with a complete variable region of the other chain, or with a portion of the other chain's variable region of similar length and having the same number of CDRs, e.g., the light chain, will allow binding, e.g., of at least 25, 50, 75, 85 or 90% of that seen with the whole heavy and light variable region. [026] As used herein, the term "human antibody" includes an antibody that possesses a sequence that is derived from a human germ-line immunoglobulin sequence, such as an antibody derived from transgenic mice having human immunoglobulin genes (e.g., XENOMOUSEm genetically engineered mice (Abgenix, Fremont, CA)), human phage display libraries, human myeloma cells, or human B cells. [027] As used herein, the term "humanized antibody" refers to an antibody that is derived from a non-human antibody e.g., rodent (e.g., murine) that retains or substantially retains the antigen-binding properties of the parent antibody but is less immunogenic in humans. Humanized as used herein is intended to include deimmunized antibodies. Typically humanized antibodies include non -human CDRs and human or human derived framework and constant regions. [028] The term "modified" antibody, as used herein, refers to antibodies that are prepared, expressed, created or isolated by recombinant means, such as antibodies 10 WO 2011/050242 PCTUS2010/053686 expressed using a recombinant expression vector transfected into a host cell, antibodies isolated from a recombinant, combinatorial antibody library, antibodies isolated from an animal (e.g., a mouse, sheep or goat) that is transgenic for human immunoglobulin genes or antibodies prepared, expressed, created or isolated by any other means that involves splicing of human immunoglobulin gene sequences to other DNA sequences. Such modified antibodies include humanized, CDR grafted (e.g., an antibody having CDRs from a first antibody and a framework region from a different source, e.g., a second antibody or a consensus framework), chimeric, in vitro generated (e.g., by phage display) antibodies, and may optionally include variable or constant regions derived from human germline immunoglobulin sequences or human immunoglobulin genes or antibodies which have been prepared, expressed, created or isolated by any means that involves splicing of human immunoglobulin gene sequences to alternative immunoglobulin sequences. In embodiments a modified antibody molecule includes an antibody molecule having a sequence change from a reference antibody. [0291 The term "monospecific antibody" refers to an antibody or antibody preparation that displays a single binding specificity and affinity for a particular epitope. This term includes a "monoclonal antibody" or "monoclonal antibody composition." [0301 The term "bispecific antibody" or "bifunctional antibody" refers to an antibody that displays dual binding specificity for two epitopes, where each binding site differs and recognizes a different epitope. [0311 The terms "non-conjugated antibody" and "naked antibody" are used interchangeably to refer to an antibody molecule that is not conjugated to a non-antibody moiety, e.g., a therapeutic agent or a label. [032] The terms "immunoconjugate", "antibody conjugate", "antibody drug conjugate", and "ADC" are used interchangeably and refer to an antibody that is conjugated to a non-antibody moiety, e.g., a therapeutic agent or a label. [0331 The term "agent" is used herein to denote a chemical compound, a mixture of chemical compounds, a biological macromolecule, or an extract made from biological materials. The term "therapeutic agent" refers to an agent that has biological activity. [034] The term "anti-cancer agent" or "chemotherapeutic agent' is used herein to refer to agents that have the functional property of inhibiting a development or progression 11 WO 2011/050242 PCTIUS2010/053686 of a neoplasm in a human, particularly a malignant (cancerous) lesion, such as a carcinoma, sarcoma, lymphoma, or leukemia. Inhibition of metastasis or angiogenesis is frequently a property of anti-cancer or chemotherapeutic agents. A chemotherapeutic agent may be a cytotoxic or cytostatic agent. The term "cytostatic agent" refers to an agent which inhibits or suppresses cell growth and/or multiplication of cells. [035] "Cytotoxic agents" refer to compounds which cause cell death primarily by interfering directly with the cell's functioning, including, but not limited to, alkylating agents, tumor necrosis factor inhibitors, intercalators, microtubule inhibitors, kinase inhibitors, proteasome inhibitors and topoisomerase inhibitors. A "toxic payload" as used herein refers to a sufficient amount of cytotoxic agent which, when delivered to a cell results in cell death. Delivery of a toxic payload may be accomplished by administration of a sufficient amount of immunoconjugate comprising an antibody or antigen binding fragment of the invention and a cytotoxic agent. Delivery of a toxic payload may also be accomplished by administration of a sufficient amount of an immunoconjugate comprising a cytotoxic agent, wherein the immunoconjugate comprises a secondary antibody or antigen binding fragment thereof which recognizes and binds an antibody or antigen binding fragment of the invention. [036] As used herein the phrase, a sequence "derived from" or "specific for a designated sequence" refers to a sequence that comprises a contiguous sequence of approximately at least 6 nucleotides or at least 2 amino acids, at least about 9 nucleotides or at least 3 amino acids, at least about 10-12 nucleotides or 4 amino acids, or at least about 15-21 nucleotides or 5-7 amino acids corresponding, i.e., identical or complementary to, e.g., a contiguous region of the designated sequence. In certain embodiments, the sequence comprises all of a designated nucleotide or amino acid sequence. The sequence may be complementary (in the case of a polynucleotide sequence) or identical to a sequence region that is unique to a particular sequence as determined by techniques known in the art. Regions from which sequences may be derived, include but are not limited to, regions encoding specific epitopes, regions encoding CDRs, regions encoding framework sequences, regions encoding constant domain regions, regions encoding variable domain regions, as well as non-translated and/or non-transcribed regions. The derived sequence will not necessarily be derived physically from the sequence of interest under study, but may be generated in any manner, including, but not limited to, chemical synthesis, replication, reverse transcription or transcription, that is based on the information provided 12 WO 2011/050242 PCT/US2010/053686 by the sequence of bases in the region(s) from which the polynucleotide is derived. As such, it may represent either a sense or an antisense orientation of the original polynucleotide. In addition, combinations of regions corresponding to that of the designated sequence may be modified or combined in ways known in the art to be consistent with the intended use. For example, a sequence may comprise two or more contiguous sequences which each comprise part of a designated sequence, and are interrupted with a region which is not identical to the designated sequence but is intended to represent a sequence derived from the designated sequence. With regard to antibody molecules, "derived therefrom" includes an antibody molecule which is functionally or structurally related to a comparison antibody, e.g., "derived therefrom"-includes an antibody molecule having similar or substantially the same sequence or structure, e.g., having the same or similar CDRs, framework or variable regions. "Derived therefrom" for an antibody also includes residues, e.g., one or more, e.g., 2, 3, 4, 5, 6 or more residues, which may or may not be contiguous, but are defined or identified according to a numbering scheme or homology to general antibody structure or three-dimensional proximity, i.e., within a CDR or a framework region, of a comparison sequence. The term "derived therefrom" is not limited to physically derived therefrom but includes generation by any manner, e.g., by use of sequence information from a comparison antibody to design another antibody. [037] As used herein, the phrase "encoded by" refers to a nucleic acid sequence that codes for a polypeptide sequence, wherein the polypeptide sequence or a portion thereof contains an amino acid sequence of at least 3 to 5 amino acids, at least 8 to 10 amino acids, or at least 15 to 20 amino acids from a polypeptide encoded by the nucleic acid sequence. [038] Calculations of "homology" between two sequences can be performed as follows. The sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in one or both of a first and a second amino acid or nucleic acid sequence for optimal alignment and non-homologous sequences can be disregarded for comparison purposes). The length of a reference sequence aligned for comparison purposes is at least 30%, 40%, or 50%, at least 60%, or at least 70%, 80%, 90%, 95%, 100% of the length of the reference sequence. The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in the first sequence is occupied by the same amino acid residue or nucleotide as the corresponding 13 WO 2011/050242 PCTUS2010/053686 position in the second sequence, then the molecules are identical at that position (as used herein amino acid or nucleic acid "identity" is equivalent to amino acid or nucleic acid "homology"). The percent identity between the two sequences is a function of the number of identical positions shared by the sequences, taking into account the number of gaps, and the length of each gap, which need to be introduced for optimal alignment of the two sequences. [039] The comparison of sequences and determination of percent homology between two sequences can be accomplished using a mathematical algorithm. The percent homology between two amino acid sequences can be determined using any method known in the art. For example, the Needleman and Wunsch, J Mol. Biol 48:444-453 (1970), algorithm which has been incorporated into the GAP program in the GCG software package , using either a Blossum 62 matrix or a PAM250 matrix, and a gap weight of 16, 14, 12, 10, 8, 6, or 4 and a length weight of 1, 2, 3, 4, 5, or 6. The percent homology between two nucleotide sequences can also be determined using the GAP program in the GCG software package (Accelerys, Inc. San Diego, CA), using an NWSgapdna.CMP matrix and a gap weight of 40, 50, 60, 70, or 80 and a length weight of 1, 2, 3, 4, 5, or 6. An exemplary set of parameters for determination of homology are a Blossum 62 scoring matrix with a gap penalty of 12, a gap extend penalty of 4, and a frameshift gap penalty of 5. [040] As used herein, the term "hybridizes under stringent conditions" describes conditions for hybridization and washing. Guidance for performing hybridization reactions can be found in Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6. Aqueous and nonaqueous methods are described in that reference and either can be used. Specific hybridization conditions referred to herein are as follows: 1) low stringency hybridization conditions in 6X sodium chloride/sodium citrate (SSC) at about 45*C, followed by two washes in 0.2X SSC, 0.1% SDS at least at 50*C (the temperature of the washes can be increased to 55"C for low stringency conditions); 2) medium stringency hybridization conditions in 6X SSC at about 45*C, followed by one or more washes in 0.2X SSC, 0.1% SDS at 60"C; 3) high stringency hybridization conditions in 6X SSC at about 45*C, followed by one or more washes in 0.2X SSC, 0.1% SDS at 65*C; and 4) very high stringency hybridization conditions are 0.5M sodium phosphate, 7% SDS at 65*C, followed by one or more washes at 0.2X SSC, 14 WO 2011/050242 PCT/US2010/053686 1% SDS at 65"C. Very high stringency conditions (4) are often the preferred conditions and the ones that should be used unless otherwise specified. .10411 It is understood that the antibodies and antigen binding fragment thereof of the invention may have additional conservative or non-essential amino acid substitutions, which do not have a substantial effect on the polypeptide functions. Whether or not a particular substitution will be tolerated, i.e., will not adversely affect desired biological properties, such as binding activity, can be determined as described in Bowie, JU et al. Science 247:1306-1310 (1990) or Padlan et al. FASEB J. 9:133-139 (1995). A "conservative amino acid substitution" is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include amino acids with basic side chains (e.g., lysine, arginine, histidine), acidic side chains (e.g., aspartic acid, glutamic acid), uncharged polar side chains (e.g., asparagine, glutamine, serine, threonine, tyrosine, cysteine), nonpolar side chains (e.g., glycine, alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), beta-branched side chains (e.g., threonine, valine, isoleucine) and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan, histidine). [042] A "non-essential" amino acid residue is a residue that can be altered from the wild-type sequence of the binding agent, e.g., the antibody, without abolishing or, without substantially altering a biological activity, whereas an "essential" amino acid residue results in such a change. In an antibody, an essential amino acid residue can be a specificity determining residue (SDR). [043] As used herein, the term "isolated" refers to material that is removed from its original environment (e.g., the natural environment if it is naturally occurring). For example, a naturally occurring polynucleotide or polypeptide present in a living animal is not isolated, but the same polynucleotide or DNA or polypeptide, separated from some or all of the coexisting materials in the natural system, is isolated. Such polynucleotide could be part of a vector and/or such polynucleotide or polypeptide could be part of a composition, e.g., a mixture, solution or suspension or comprising an isolated cell or a cultured cell which comprises the polynucleotide or polypeptide, and still be isolated in that the vector or composition is not part of its natural environment. 15 WO 2011/050242 PCT/US2010/053686 [044] As used herein, the term "replicon" refers to any genetic element, such as a plasmid, a chromosome or a virus, that behaves as an autonomous unit ofpolynucleotide replication within a cell. [045] As used herein, the term "operably linked" refers to a situation wherein the components described are in a relationship permitting them to function in their intended manner. Thus, for example, a control sequence "operably linked" to a coding sequence is ligated in such a mamer that expression of the coding sequence is achieved under conditions compatible with the control sequence. [0461 As used herein, the term "vector" refers to a replicon in which another polynucleotide segment is attached, such as to bring about the replication and/or expression of the attached segment. [047] As used herein, the term "control sequence" refers to a polynucleotide sequence that is necessary to effect the expression of a coding sequence to which it is ligated. The nature of such control sequences differs depending upon the host organism. In prokaryotes, such control sequences generally include a promoter, a ribosomal binding site and terminators and, in some instances, enhancers. The term "control sequence" thus is intended to include at a minimum all components whose presence is necessary for expression, and also may include additional components whose presence is advantageous, for example, leader sequences. [048] As used herein, the term "purified product" refers to a preparation of the product which has been isolated from the cellular constituents with which the product is normally associated and/ or from other types of cells that may be present in the sample of interest. [049] As used herein, the term "epitope" refers to a protein determinate capable of binding specifically to an antibody. Epitopic determinants usually consist of chemically active surface groupings of molecules such as amino acids or sugar side chains and usually have specific three dimensional structural characteristics, as well as specific charge characteristics. Some epitopes are linear epitopes while others are conformational epitopes. A linear epitope is an epitope wherein a contiguous amino acid primary sequence comprises the epitope recognized. A linear epitope typically includes at least 3, and more usually, at least 5, for example, about 8 to about 10 contiguous amino acids. A conformational epitope can result from at least two situations, such as: a) a linear sequence 16 WO 2011/050242 PCTUS2010/053686 which is only exposed to antibody binding in certain protein conformations, e.g., dependent on ligand binding, or dependent on modification (e.g., phosphorylation) by signaling molecules; or b) a combination of structural features from more than one part of the protein, or in multisubunit proteins, from more than one subunit, wherein the features are in sufficiently close proximity in 3-dimensional space to participate in binding. 10501 As used herein, "isotype" refers to the antibody class (e.g., IgM or IgGl) that is encoded by heavy chain constant region genes. [051] As used herein, the terms "detectable agent," "label" or "labeled" are used to refer to incorporation of a detectable marker on a polypeptide or glycoprotein. Various methods of labeling polypeptides and glycoproteins are known in the art and may be used. Examples of labels for polypeptides include, but are not limited to, the following: radioisotopes or radionuclides (e.g., indium ("In), iodine (131J or 5 I), yttrium ("Y), lutetium (177Lu), actinium ( 2 25 Ac), bismuth ( 2 Bi or 21 3 Bi), sulfur ("S), carbon (1 4 C), tritium (3H), rhodium ("'Rh), technetium ( 9 mTc), praseodymium, or phosphorous ( 32 P) or a positron-emitting radionuclide, e.g., carbon- 11 ("C), potassium-40 ( 40 K), nitrogen-1 3 ("N), oxygen-15 ('0), fluorine-18 (18F), and iodine-121 (121)), fluorescent labels (e.g., FITC, rhodamine, lanthanide phosphors), enzymatic labels (e.g., horseradish peroxidase, beta-galactosidase, luciferase, alkaline phosphatase), chemiluminescent, biotinyl groups (which can be detected by a marked avidin, e.g., a molecule containing a streptavidin moiety and a fluorescent marker or an enzymatic activity that can be detected by optical or calorimetric methods), and predetermined polypeptide epitopes recognized by a secondary reporter (e.g., leucine zipper pair sequences, binding sites for secondary antibodies, metal binding domains, epitope tags). In some embodiments, labels are attached by spacer arms of various lengths to reduce potential steric hindrance. [0521 As used herein, "specific binding," "bind(s) specifically" or "binding specificity" means, for an anti-GCC antibody molecule, that the antibody molecule binds to GCC, e.g., human GCC protein, with greater affinity than it does to a non-GCC protein, e.g., BSA. Typically an anti-GCC molecule will have a K for the non-GCC protein, e.g., BSA, which is greater than 2, greater than 10, greater than 100, greater than 1,000 times, greater than 104 , greater than 10 5 , or greater than 106 times its K 4 for GCC, e.g., human GCC protein. In determination of Kd, the Kd for GCC and the non-GCC protein, e.g., BSA, should be done under the same conditions. 17 WO 2011/050242 PCT/US2010/053686 [053] As used herein, the term "treat" or "treatment" is defined as the administration of an anti-GCC antibody molecule to a subject, e.g., a patient, or administration, e.g., by application, to an isolated tissue or cell from a subject which is returned to the subject. The anti-GCC antibody molecule can be administered alone or in combination with a second agent. The treatment can be to cure, heal, alleviate, relieve, alter, remedy, ameliorate, palliate, improve or affect the disorder, the symptoms of the disorder or the predisposition toward the disorder, e.g., a cancer. While not wishing to be bound by theory, treating is believed to cause the inhibition, ablation, or killing of a cell in vitro or in vivo, or otherwise reducing capacity of a cell, e.g., an aberrant cell, to mediate a disorder, e.g., a disorder as described herein (e.g., a cancer). [054] As used herein, the term "subject" is intended to include mammals, primates, humans and non-human animals. For example, a subject can be a patient (e.g., a human patient or a veterinary patient), having a cancer, e.g., of gastrointestinal origin (e.g., colon cancer), a symptom of a cancer, e.g., of gastrointestinal origin (e.g., colon cancer), in which at least some of the cells express GCC, or a predisposition toward a cancer, e.g., of gastrointestinal origin (e.g., colon cancer), in which at least some of the cells express GCC. The term "non-human animals" of the invention includes all non-human vertebrates, e.g., non-human mammals and non-mammals, such as non-human primates, sheep, dog, cow, chickens, amphibians, reptiles, etc, unless otherwise noted. In an embodiment subject excludes one or more or all of a mouse, rat, rabbit or goat. [0551 As used herein, an amount of an anti-GCC antibody molecule "effective" or "sufficient" to treat a disorder, or a "therapeutically effective amount" or "therapeutically sufficient amount" refers to an amount of the antibody molecule which is effective, upon single or multiple dose administration to a subject, in treating a cell, e.g., cancer cell (e.g., a GCC-expressing tumor cell), or in prolonging curing, alleviating, relieving or improving a subject with a disorder as described herein beyond that expected in the absence of such treatment. As used herein, "inhibiting the growth" of the tumor or cancer refers to slowing, interrupting, arresting or stopping its growth and/or metastases and does not necessarily indicate a total elimination of the tumor growth. [056] As used herein, "GCC," also known as "STAR", "GUC2C", "GUCY2C" or "ST receptor" protein refers to mammalian GCC, preferably human GCC protein. Human GCC refers to the protein shown in SEQ ID NO:228 and naturally occurring allelic protein 18 WO 2011/050242 PCT/US2010/05368 6 variants thereof. The allele in SEQ ID NO: 228 can be encoded by the nucleic acid sequence of GCC shown in SEQ ID NO:227. Other variants are known in the art. See, e.g., accession number Ensp000261170, Ensembi Database, European Bioinformatics Institute and Wellcome Trust Sanger Institute, which has a leucine at residue 281; SEQ ID NO: 14 of published US patent application number US 20060035852; or GenBank accession number AAB 19934. Typically, a naturally occurring allelic variant has an amino acid sequence at least 95%, 97% or 99% identical to the GCC sequence of SEQ ID NO:228. The transcript encodes a protein product of 1073 amino acids, and is described in GenBank accession no.: NM_004963. GCC protein is characterized as a transmembrane cell surface receptor protein, and is believed to play a critical role in the maintenance of intestinal fluid, electrolyte homeostasis and cell proliferation. [0571 Unless otherwise noted, the term "alkyl" refers to a saturated straight or branched hydrocarbon having from about I to about 20 carbon atoms (and all combinations and subcombinations of ranges and specific numbers of carbon atoms therein), with from about I to about 8 carbon atoms being preferred. Examples of alkyl groups are methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl, tert-butyl, n pentyl, 2-pentyl, 3-pentyl, 2-methyl-2-butyl, n-hexyl, n-heptyl, n-octyl, n-nonyl, n-decyl, 3-methyl-2-butyl, 3-methyl-1-butyl, 2-methyl-1-butyl, 1-hexyl, 2-hexyl, 3-hexyl, 2 methyl-2-pentyl, 3-methyl-2-pentyl, 4-methyl-2-pentyl, 3-methyl-3-pentyl, 2-methyl-3 pentyl, 2,3-dimethyl-2-butyl, and 3,3-dimethyl-2-butyl. [058] Alkyl groups, whether alone or as part of another group, may be referred to as "substituted." A substituted alkyl group is an alkyl group that is substituted with one or more groups, preferably 1 to 3 groups (and any additional substituents selected from halogen), including, but not limited to, -halogen, -O-(C-Cs alkyl), -O-(C 2 -Cg alkenyl), 0-(C 2
-C
8 alkynyl), -aryl, -C(O)R', -OC(O)R', -C(O)OR', -C(O)NH 2 , -C(O)NHR', C(O)N(R') 2 , -NHC(O)R', -SR', -S0 3 R', -S(O) 2 R', -S(O)R', -OH, =0, -N 3 , -NH 2 , NH(R'), -N(R') 2 and -CN, where each R' is independently selected from -H, -Ci-Cs alkyl,
-C
2 -Cs alkenyl, -C 2
-C
8 alkynyl, or -aryl, and wherein said -O-(C-C 8 alkyl), -O-(C 2 -Cs alkenyl), -O-(C 2
-C
8 alkynyl), -aryl, -C-Cs alkyl, -C 2 -Cs alkenyl, and -C 2
-C
8 alkynyl groups can be optionally further substituted with one or more groups including, but not limited to, -C-C 8 alkyl, -C 2 -Cs alkenyl, -C 2
-C
8 alkynyl, -halogen, -O-(C-C 8 alkyl), -0
(C
2 -Cs alkenyl), -O-(C 2
-C
8 alkynyl), -aryl, -C(O)R", -OC(O)R", -C(O)OR", -C(O)NH 2 , -C(O)NHR", -C(O)N(R")2, -NHC(O)R", -SR", -SO 3 R", -S(O) 2 R", -S(O)R", -OH, -N 3 19 WO 2011/050242 PCT/US2010/053686 , -NH2, -NH(R"), -N(R")2 and -CN, where each R" is independently selected from -H, Ci-C 8 alkyl, -C 2
-C
8 alkenyl, -C 2
-C
8 alkynyl, or -aryl. [0591 Unless otherwise noted, the terms "alkenyl" and "alkynyl" refer to straight and branched carbon chains having from about 2 to about 20 carbon atoms (and all combinations and subcombinations of ranges and specific numbers of carbon atoms therein), with from about 2 to about 8 carbon atoms being preferred. An alkenyl chain has at least one double bond in the chain and an alkynyl chain has at least one triple bond in the chain. Examples of alkenyl groups include, but are not limited to, ethylene or vinyl, allyl, -1-butenyl, -2-butenyl, -isobutylenyl, -1-pentenyl, -2-pentenyl, -3-methyl-1-butenyl, -2-methyl-2-butenyl, and -2,3-dimethyl-2-butenyl. Examples of alkynyl groups include, but are not limited to, acetylenic, propargyl, acetylenyl, propynyl, -1-butynyl, -2-butynyl, -1-pentynyl, -2-pentynyl, and -3-methyl-i butynyl. [060] As with alkyl groups, alkenyl and alkynyl groups, can be substituted. A "substituted" alkenyl or alkynyl group is one that is substituted with one or more groups, preferably 1 to 3 groups (and any additional substituents selected from halogen), including but not limited to, -halogen, -O-(C 1 -Cs alkyl), -O-(C 2 -Cs alkenyl), -O-(C 2 -Cs alkynyl), aryl, -C(O)R', -OC(O)R', -(O)OR', -C(O)NH 2 , -C(O)NHR', -C(O)N(R') 2 , NHC(O)R', -SR', -SO 3 R', -S(O) 2 R', -S(O)R', -OH, =0, -N 3 , -NH2, -NH(R'), -N(R') 2 and -CN, where each R' is independently selected from -H, -C1-Cs alkyl, -C 2
-C
8 alkyenl, C 2
-C
8 alkynyl, or -aryl and wherein said -O-(C1-C8 alkyl), -O-(C 2
-C
8 alkenyl), -O-(C2-Cs alkynyl), -aryl, -C 1
-C
8 alkyl, -C 2
-C
8 alkenyl, and -C 2
-C
8 ailcynyl groups can be optionally further substituted with one or more substituents including, but not limited to, -C 1-Cs alkyl,
-C
2 -CS alkenyl, -C 2
-C
8 alkynyl, -halogen, -O-(CI-Cs alkyl), -O-(C 2 -Cs alkenyl), -O-(C 2 Cs alkynyl), -aryl, -C(O)R", -OC(O)R", -C(O)OR", -C(0)NH2, -C(O)NHR", -C(O)N(R")2, -NHC(O)R", -SR", -SO 3 R", -S(O) 2 R", -S(O)R", -OH, -N 3 , -NH 2 , -NH(R"), -N(R")2 and -CN, where each R" is independently selected from -H, -C 1
-C
8 alkyl, -C 2
-C
8 alkenyl, C 2 -Cs alkynyl, or -aryl. [0611 Unless otherwise noted, the term "alkylene" refers to a saturated branched or straight chain hydrocarbon radical having from about 1 to about 20 carbon atoms (and all combinations and subcombinations of ranges and specific numbers of carbon atoms therein), with from about I to about 8 carbon atoms being preferred and having two monovalent radical centers derived by the removal of two hydrogen atoms from the same 20 WO 2011/050242 PCT/US2010/053686 or two different carbon atoms of a parent alkane. Typical alkylenes include, but are not limited to, methylene, ethylene, propylene, butylene, pentylene, hexylene, heptylene, ocytylene, nonylene, decalene, 1,4-cyclohexylene, and the like. Alkylene groups, whether alone or as part of another group, can be optionally substituted with one or more groups, preferably 1 to 3 groups (and any additional substituents selected from halogen), including, but not limited to, -halogen, -O-(C-C 8 alkyl), -O-(C 2 -Cs alkenyl), -O-(C 2 -Cs alkynyl), -aryl, -C(O)R', -OC(O)R', -C(O)OR', -C(O)NH 2 , -C(O)NHR', -C(O)N(R') 2 , NHC(O)R', -SR', -SO 3 R', -S(O) 2 R', -S(O)R', -OH, =0, -N 3 , -NH 2 , -NH(R'), -N(R') 2 and -CN, where each R' is independently selected from -H, -C-C 8 alkyl, -CrCs alkenyl, -C 2 Cs alkynyl, or -aryl and wherein said -O-(C-C 8 alkyl), -O-(C 2 -Cs alkenyl), -O-(C 2 -Cs alkynyl), -aryl, -C 1
-C
8 alkyl, -C 2 -Cs aLkenyl, and -C 2
-C
8 alkynyl groups can be further optionally substituted with one or more substituents including, but not limited to, -C 1
-C
8 alkyl, -C 2
-C
8 alkenyl, -C 2
-C
8 alkynyl, -halogen, -O-(C-Cs alkyl), -O-(C 2 -Cs alkenyl), -0
(C
2
-C
8 alkynyl), -aryl, -C(O)R", -OC(O)R", -C(O)OR", -C(O)NH 2 , -C(O)NHR", C(O)N(R") 2 , -NHC(O)R", -SR", -SO 3 R", -S(O) 2 R", -S(O)R", -OH, -N 3 , -NH 2 , NH(R"), -N(R")2 and -CN, where each R" is independently selected from -H, -Cr-Cs alkyl, -C 2 -Cs alkenyl, -CrCs alkynyl, or -aryl. [062] Unless otherwise noted, the term "alkenylene" refers to an optionally substituted alkylene group containing at least one carbon-carbon double bond. Exemplary alkenylene groups include, for example, ethenylene (-CH=CH-) and propenylene (
CH=CHCH
2 -). [063] Unless otherwise noted, the term "alkynylene" refers to an optionally substituted alkylene group containing at least one carbon-carbon triple bond. Exemplary alkynylene groups include, for example, acetylene (-C-C-), propargyl (-CH 2 C=C-), and 4 pentynyl (-CH 2 CH2CH 2 C=CH-). [064] Unless otherwise noted, the term "aryl" refers to a monovalent aromatic hydrocarbon radical of 6-20 carbon atoms (and all combinations and subcombinations of ranges and specific numbers of carbon atoms therein) derived by the removal of one hydrogen atom from a single carbon atom of a parent aromatic ring system. Some aryl groups are represented in the exemplary structures as "Ar". Typical aryl groups include, but are not limited to, radicals derived from benzene, substituted benzene, phenyl, naphthalene, anthracene, biphenyl, and the like. 21 WO 2011/050242 PCT/US2010/053686 [065] An aryl group, whether alone or as part of another group, can be optionally substituted with one or more, preferably 1 to 5, or even I to 2 groups including, but not limited to, -halogen, -C-C 8 alkyl, -C 2
-C
8 alkenyl, -C 2
-C
8 alkynyl, -O-(C-C 8 alkyl), -0
(C
2
-C
8 alkenyl), -O-(C 2
-C
8 alkynyl), -aryl, -C(O)R', -OC(O)R', -C(O)OR', -C(O)NH2, -C(O)NHR', -C(O)N(R') 2 , -NHC(O)R', -SR', -SO 3 R', -S(0) 2 R', -S(O)R', -OH, -NO 2 , -N 3 , -NH 2 , -NH(R'), -N(R') 2 and -CN, where each R' is independently selected from -H, -C
C
8 alkyl, -C2-C alkenyl, -C 2
-C
8 alkynyl, or -aryl and wherein said -C-C 8 alkyl, -C 2
-C
8 alkenyl, -C 2 -Cs alkynyl, O-(C-Cs alkyl), -O-(C 2
-C
8 alkenyl), -O-(C 2 -Cs alkynyl), and -aryl groups can be further optionally substituted with one or more substituents including, but not limited to, -CI-C 8 alkyl, -C 2
-C
8 alkenyl, -C 2 -C alkynyl, -halogen, -O-(CI-Cs alkyl), O-(C 2
-C
8 alkenyl), -0-(C 2
-C
8 alkynyl), -aryl, -C(O)R", -OC(O)R", -C(O)OR", C(O)NH 2 , -C(O)NHR", -C(O)N(R") 2 , -NHC(O)R", -SR", -SO 3 R", -S(O) 2 R", S(O)R", -OH, -N 3 , -NH 2 , -NH(R"), -N(R") 2 and -CN, where each R" is independently selected from -H, -C-C 8 alkyl, -C 2
-C
8 alkenyl, -C 2 -Cs alkynyl, or -aryl. [066] Unless otherwise noted, the term "arylene" refers to an optionally substituted aryl group which is divalent (i.e., derived by the removal of two hydrogen atoms from the same or two different carbon atoms of a parent aromatic ring system) and can be in the ortho, meta, or para configurations as shown in the following structures with phenyl as the exemplary aryl group: Typical "-(CI-C 8 alkylene)aryl," "-(C 2
-C
8 alkenylene)aryl", "and -(C 2
-C
8 alkynylene)aryl" groups include, but are not limited to, benzyl, 2-phenylethan-1-yl, 2-phenylethen-1-yl, naphthylmethyl, 2-naphthylethan-1-yl, 2-naphthylethen-1-yl, naphthobenzyl, 2 naphthophenylethan-I-yl and the like. [067] Unless otherwise noted, the term "heterocycle," refers to a monocyclic, bicyclic, or polycyclic ring system having from 3 to 14 ring atoms (also referred to as ring members) wherein at least one ring atom in at least one ring is a heteroatom selected from N, 0, P, or S (and all combinations and subcombinations of ranges and specific numbers of carbon atoms and heteroatoms therein). The heterocycle can have from 1 to 4 ring heteroatoms independently selected from N, 0, P, or S. One or more N, C, or S atoms in a 22 WO 2011/050242 PCTUS2010/053686 heterocycle can be oxidized. A monocylic heterocycle preferably has 3 to 7 ring members (e.g., 2 to 6 carbon atoms and I to 3 heteroatoms independently selected from N, 0, P, or S), and a bicyclic heterocycle preferably has 5 to 10 ring members (e.g., 4 to 9 carbon atoms and 1 to 3 heteroatoms independently selected from N, 0, P, or S). The ring that includes the heteroatom can be aromatic or non-aromatic. Unless otherwise noted, the heterocycle is attached to its pendant group at any heteroatom or carbon atom that results in a stable structure. [068] Heterocycles are described in Paquette, "Principles of Modern Heterocyclic Chemistry" (W.A. Benjamin, New York, 1968), particularly Chapters 1, 3, 4, 6, 7, and 9; "The Chemistry of Heterocyclic Compounds, A series of Monographs" (John Wiley & Sons, New York, 1950 to present), in particular Volumes 13, 14, 16, 19, and 28; and J. Am. Chem. Soc. 82:5566 (1960). [069] Unless otherwise noted, the term "heterocyclo" refers to an optionally substituted heterocycle group as defined above that is divalent (i.e., derived by the removal of two hydrogen atoms from the same or two different carbon atoms of a parent heterocyclic ring system). [070] Examples of "heterocycle" groups include by way of example and not limitation pyridyl, dihydropyridyl, tetrahydropyridyl (piperidyl), thiazolyl, pyrimidinyl, furanyl, thienyl, pyrrolyl, pyrazolyl, imidazolyl, tetrazolyl, benzofuranyl, thianaphthalenyl, indolyl, indolenyl, quinolinyl, isoquinolinyl, benzimidazolyl, piperidinyl, 4-piperidonyl, pyrrolidinyl, 2-pyrrolidonyl, pyrrolinyl, tetrahydrofuranyl, bis-tetrahydrofuranyl, tetrahydropyranyl, bis-tetrahydropyranyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, decahydroquinolinyl, octahydroisoquinolinyl, azocinyl, triazinyl, 6H-1,2,5-thiadiazinyl, 2H,6H-1,5,2-dithiazinyl, thienyl, thianthrenyl, pyranyl, isobenzofuranyl, chromenyl, xanthenyl, phenoxathinyl, 2H-pyrrolyl, isothiazolyl, isoxazolyl, pyrazinyl, pyridazinyl, indolizinyl, isoindolyl, 3H-indolyl, 1 H-indazolyl, purinyl, 4H-quinolizinyl, phthalazinyl, naphthyridinyl, quinoxalinyl, quinazolinyl, cinnolinyl, pteridinyl, 4H-carbazolyl, carbazolyl, p-carbolinyl, phenanthridinyl, acridinyl, pyrimidinyl, phenanthrolinyl, phenazinyl, phenothiazinyl, furazanyl, phenoxazinyl, isochromanyl, chromanyl, imidazolidinyl, imidazolinyl, pyrazolidinyl, pyrazolinyl, piperazinyl, indolinyl, isoindolinyl, quinuclidinyl, morpholinyl, oxazolidinyl, benzotriazolyl, benzisoxazolyl, oxindolyl, benzoxazolinyl, and isatinoyl. Preferred "heterocycle" groups include, but are 23 WO 2011/050242 PCT/US2010/053686 not limited to, benzofuranyl, benzothiophenyl, indolyl, benzopyrazolyl, coumarinyl, isoquinolinyl, pyrrolyl, thiophenyl, furanyl, thiazolyl, imidazolyl, pyrazolyl, triazolyl, quinolinyl, pyrimidinyl, pyridinyl, pyridonyl, pyrazinyl, pyridazinyl, isothiazolyl, isoxazolyl and tetrazolyl. [071] A hetero cycle group, whether alone or as part of another group, can be optionally substituted with one or more groups, preferably 1 to 2 groups, including but not limited to, -C-C 8 alkyl, -C 2 -Cs alkenyl, -C 2
-C
8 alkynyl, -halogen, -O-(CI-C 8 alkyl), -0
(C
2 -Cs alkenyl), -O-(C 2
-C
8 alkynyl), -aryl, -C(O)R', -OC(O)R', -C(O)OR', -C(O)NH 2 , C(O)NHR', -C(O)N(R') 2 , -NHC(O)R', -SR', -SO 3 R', -S(0) 2 R', -S(O)R', -OH, -N 3 , NH2, -NH(R'), -N(R') 2 and -CN, where each R' is independently selected from -H, -C-C 8 alkyl, -C 2
-C
8 aMkeny, -C 2
-C
8 alkynyl, or -aryl and wherein said -O-(C-C alkyl), -O-(C 2 CS alkenyl), -O-(C 2 -CS alkynyl), -C 1
-C
8 alkyl, -C 2 -Cs alkenyl, -C 2
-C
8 alkynyl, and -aryl groups can be further optionally substituted with one or more substituents including, but not limited to, -C-Cs ailcyl, -C 2
-C
8 alkenyl, -C 2
-C
8 alkynyl, -halogen, -O-(C-Cs alkyl), O-(C 2
-C
8 alkenyl), -O-(C 2 -Cs alkynyl), -aryl, -C(O)R", -OC(O)R", -C(O)OR", C(O)NH 2 , -C(O)NHR", -C(O)N(R") 2 , -NHC(O)R", -SR", -SO 3 R", -S(0) 2 R", S(O)R", -OH, -N 3 , -NH 2 , -NH(R"), -N(R") 2 and -CN, where each R" is independently selected from -H, -C-C 8 alkyl, -C 2
-C
8 alkenyl, -C 2 -Cs alkynyl, or aryl. [0721 By way of example and not limitation, carbon-bonded heterocycles can be bonded at the following positions: position 2, 3, 4, 5, or 6 of a pyridine; position 3, 4, 5, or 6 of a pyridazine; position 2, 4, 5, or 6 of a pyrimidine; position 2, 3, 5, or 6 of a pyrazine; position 2, 3, 4, or 5 of a furan, tetrahydrofuran, thiofuran, thiophene, pyrrole or tetrahydropyrrole; position 2, 4, or 5 of an oxazole, imidazole or thiazole; position 3, 4, or 5 of an isoxazole, pyrazole, or isothiazole; position 2 or 3 of an aziridine; position 2, 3, or 4 of an azetidine; position 2, 3, 4, 5, 6, 7, or 8 of a quinoline; or position 1, 3, 4, 5, 6, 7, or 8 of an isoquinoline. Still more typically, carbon bonded heterocycles include 2-pyridyl, 3-pyridyl, 4-pyridyl, 5-pyridyl, 6-pyridyl, 3-pyridazinyl, 4-pyridazinyl, 5-pyridazinyl, 6 pyridazinyl, 2-pyrimidinyl, 4-pyrimidinyl, 5-pyrimidinyl, 6-pyrimidinyl, 2-pyrazinyl, 3 pyrazinyl, 5-pyrazinyl, 6-pyrazinyl, 2-thiazolyl, 4-thiazolyl, or 5-thiazolyl. [073] By way of example and not limitation, nitrogen bonded heterocycles can be bonded at position 1 of an aziridine, azetidine, pyrrole, pyrrolidine, 2-pyrroline, 3 pyrroline, imidazole, imidazolidine, 2-imidazoline, 3-imidazoline, pyrazole, pyrazoline, 2 24 WO 2011/050242 PCTUS2010/053686 pyrazoline, 3-pyrazoline, piperidine, piperazine, indole, indoline, or 1H-indazole; position 2 of a isoindole, or isoindoline; position 4 of a morpholine; and position 9 of a carbazole, or p-carboline. Still more typically, nitrogen bonded heterocycles include 1-aziridyl, 1 azetedyl, 1-pyrrolyl, 1-imidazolyl, 1-pyrazolyl, and 1-piperidinyl. [0741 Unless otherwise noted, the term "carbocycle," refers to a saturated or unsaturated non-aromatic monocyclic, bicyclic, or polycyclic ring system having from 3 to 14 ring atoms (and all combinations and subcombinations of ranges and specific numbers of carbon atoms therein) wherein all of the ring atoms are carbon atoms. Monocyclic carbocycles preferably have 3 to 6 ring atoms, still more preferably 5 or 6 ring atoms. Bicyclic carbocycles preferably have 7 to 12 ring atoms, e.g., arranged as a bicyclo [4,5], [5,5], [5,6] or [6,6] system, or 9 or 10 ring atoms arranged as a bicyclo [5,6] or [6,6] system. The term "carbocycle" includes, for example, a monocyclic carbocycle ring fused to an aryl ring (e.g., a monocyclic carbocycle ring fused to a benzene ring). Carbocyles preferably have 3 to 8 carbon ring atoms. [0751 Carbocycle groups, whether alone or as part of another gmup, can be optionally substituted with, for example, one or more groups, preferably 1 or 2 groups (and any additional substituents selected from halogen), including, but not limited to, -halogen, -C-Cs alkyl, -C 2
-C
8 alkenyl, -C 2 -Cs alkynyl, -O-(C-Cs alkyl), -O-(C 2
-C
8 alkenyl), -0
(C
2
-C
8 alkynyl), -aryl, -C(O)R', -OC(O)R', -C(O)OR', -C(O)NH 2 , -C(O)NHR', C(O)N(R') 2 , -NHC(O)R', -SR', -SO 3 R', -S(O) 2 R', -S(O)R', -OH, =0, -N 3 , -NH2, NH(R'), -N(R') 2 and -CN, where each R' is independently selected from -H, -C-C 8 alkyl,
-C
2
-C
8 alkenyl, -7 2
-C
8 alkynyl, or -aryl and wherein said -C-C 8 alkyl, -C 2
-C
8 alkenyl, C-Cs alkynyl, -O-(CI-Cs alkyl), -O-(C 2 -Cs alkenyl), -O-(C 2 -Cs alkynyl), and -aryl groups can be further optionally substituted with one or more substituents including, but not limited to, -C-Cs alkyl, -C 2
-C
8 alkenyl, -C-Cs alkynyl, -halogen, -O-(C-C 8 alkyl), -0
(C
2
-C
8 alkenyl), -0-(CrCs alkynyl), -aryl, -C(O)R", -OC(O)R", -C(O)OR", -C(O)NH2, -C(O)NHR", -C(O)N(R") 2 , -NHC(O)R", -SR", -SO 3 R", -S(O) 2 R", -S(O)R", -OH, -N 3 -NH2, -NH(R"), -N(R")2 and -CN, where each R" is independently selected from -H, C 1
-C
8 alkyl, -C 2
-C
8 alkenyl, -C 2 -Cs alkynyl, or -aryl. [076] Examples of monocyclic carbocylic substituents include -cyclopropyl, -cyclobutyl, -cyclopentyl, -1-cyclopent-1-enyl, -1-cyclopent-2-enyl, -1-cyclopent-3-enyl, cyclohexyl, -1-cyclohex-1-enyl, -1-cyclohex-2-enyl, -1-cyclohex-3-enyl, -cycloheptyl, 25 WO 2011/050242 PCTUS2010/053686 -cyclooctyl. -1,3-cyclohexadienyl, -1,4-cyclohexadienyl, -1,3-cycloheptadienyl, -1,3,5 cycloheptatrienyl, and -cyclooctadienyl. [077] A "carbocyclo," whether used alone or as part of another group, refers to an optionally substituted carbocycle group as defined above that is divalent (i.e., derived by the removal of two hydrogen atoms from the same or two different carbon atoms of a parent carbocyclic ring system). [078] Unless otherwise indicated by context, a hyphen (-) designates the point of attachment to the pendant molecule. Accordingly, the term "-(C1-Cs alkylene)aryl" or "-Cl-Cs alkylene(aryl)" refers to a CI-C alkylene radical as defined herein wherein the alkylene radical is attached to the pendant molecule at any of the carbon atoms of the alkylene radical and one of the hydrogen atoms bonded to a carbon atom of the alkylene radical is replaced with an aryl radical as defined herein. [079] When a particular group is "substituted", that group may have one or more substituents, preferably from one to five substituents, more preferably from one to three substituents, most preferably from one to two substituents, independently selected from the list of substituents. The group can, however, generally have any number of substituents selected from halogen. Groups that are substituted are so indicated. [080] It is intended that the definition of any substituent or variable at a particular location in a molecule be independent of its definitions elsewhere in that molecule. It is understood that substituents and substitution patterns on the compounds of this invention can be selected by one of ordinary skill in the art to provide compounds that are chemically stable and that can be readily synthesized by techniques known in the art as well as those methods set forth herein. [081] Protective groups as used herein refer to groups which selectively block, either temporarily or permanently, one reactive site in a multifunctional compound. Suitable hydroxy-protecting groups for use in the present invention are pharmaceutically acceptable and may or may not need to be cleaved from the parent compound after administration to a subject in order for the compound to be active. Cleavage is through normal metabolic processes within the body. Hydroxy protecting groups are well known in the art, see, PROTECTIVE GROUPS IN ORGANIC SYNTHESIs by T. W. Greene and P. G. M. Wuts (John Wiley & sons, 3 d Edition) incorporated herein by reference in its entirety and 26 WO 2011/050242 PCT/JS2010/053686 for all purposes and include, for example, ether (e.g., alkyl ethers and silyl ethers including, for example, dialkylsilylether, trialkylsilylether, dialkylalkoxysilylether), ester, carbonate, carbamates, sulfonate, and phosphate protecting groups. Examples of hydroxy protecting groups include, but are not limited to, methyl ether; methoxymethyl ether, methylthiomethyl ether, (phenyldimethylsilyl)methoxymethy ether, benzyloxymethyl ether, p-methoxybenzyloxymethyl ether, p-nitrobenzyloxymethyl ether, o nitrobenzyloxymethyl ether, (4-methoxyphenoxy)methyl ether, guaiacolmethyl ether, t butoxymethyl ether, 4-pentenyloxymethyl ether, siloxymethyl ether, 2 methoxyethoxymethyl ether, 2,2,2-trichloroethoxymethyl ether, bis(2-chlomethoxy)methyl ether, 2-(trimethylsilyl)ethoxymethyl ether, menthoxymethyl ether, tetrahydropyranyl ether, 1-methoxycylcohexyl ether, 4-methoxytetrahydrothiopyranyl ether, 4 methoxytetrahydrothiopyranyl ether S,S-Dioxide, 1-[(2-choro-4-methyl)phenyl]-4 methoxypiperidin-4-yl ether, 1-(2-fluorophneyl)-4-methoxypiperidin-4-yI ether, 1,4 dioxan-2-yl ether, tetrahydrofuranyl ether, tetrahydrothiofuranyl ether; substituted ethyl ethers such as 1 -ethoxyethyl ether, 1-(2-chloroethoxy)ethyl ether, 1-[2 (trimethylsilyl)ethoxy]ethyl ether, 1-methyl-1-methoxyethyl ether, 1-methyl-1 benzyloxyethyl ether, 1-methyl-1-benzyloxy-2-fluoroethyl ether, 1-methyl-lphenoxyethyl ether, 2-trimethylsilyl ether, t-butyl ether, allyl ether, propargyl ethers, p-chlorophenyl ether, p-methoxyphenyl ether, benzyl ether, p-methoxybenzyl ether 3,4-dinethoxybenzyl ether, trimethylsilyl ether, triethylsilyl ether, tripropylsilylether, dimethylisopropylsilyl ether, diethylisopropylsilyl ether, dimethylhexylsilyl ether, t-butyldimethylsilyl ether, diphenylmethylsilyl ether, benzoylformate ester, acetate ester, chloroacetate ester, dichloroacetate ester, trichloroacetate ester, trifluoroacetate ester, methoxyacetate ester, triphneylmethoxyacetate ester, phenylacetate ester, benzoate ester, alkyl methyl carbonate, alkyl 9-fluorenylmethyl carbonate, alkyl ethyl carbonate, alkyl 2,2,2,-trichloroethyl carbonate, 1,1,-dimethyl-2,2,2-trichloroethyl carbonate, alkylsulfonate, methanesulfonate, benzylsulfonate, tosylate, methylene acetal, ethylidene acetal, and t-butylmethylidene ketal. Preferred protecting groups are represented by the formulas -R, -Si(R)(R)(R), -C(O)R, -C(O)OR, -C(O)NH(R), -S(0) 2 R, -S(O) 2 0H, P(O)(OH) 2 , and -P(O)(OH)OR, wherein R is C 1
-C
20 alkyl, C 2
-C
20 alkenyl, C 2
-C
20 alkynyl, -CI-C 2 0 alkylene(carbocycle),
-C
2
-C
20 alkenylene(carbocycle), -C 2
-C
20 alkynylene(carbocycle), -C 6
-C
10 aryl, -CrC 2 0 alkylene(aryl), -C 2
-C
20 alkenylene(aryl), -C 2
-C
20 alkynylene(aryl), -C-C 20 alkylene(heterocycle), -C 2
-C
20 alkenylene(heterocycle), or -C 2
-C
2 0 alkynylene(heterocycle) 27 WO 2011/050242 PCTUS2010/053686 wherein said alkyl, alkenyl, alkynyl, alkylene, alkenylene, alkynylene, aryl, carbocycle, and heterocycle radicals whether alone or as part of another group are optionally substituted. [082] The abbreviation "AFP" refers to dimethylvaline-valine-dolaisoleuine dolaproine-phenylalanine-p-phenylenediamine (see Formula (XVIII) infra). [083] The abbreviation "MMAE" refers to monomethyl auristatin E (see Formula (MII) infra). [084] The abbreviation "AEB" refers to an ester produced by reacting auristatin E with paraacetyl benzoic acid (see Formula (XX1) infra). [085] The abbreviation "AEVB" refers to an ester produced by reacting auristatin E with benzoylvaleric acid (see Formula (XMII) infra). [086) The abbreviation "MMAF" refers to monomethyl auristatin F (see Formula (X!) infra). Antibodies [087] In certain aspects, the invention relates to anti-GCC antibody molecules with features such as those summarized in Tables 1 and 2. In other aspects, the invention relates to anti-GCC antibody molecules with features such as those summarized in Tables 3, 4, 5 and/or 6. [0881 In an embodiment, the anti-GCC antibody molecule is a human hybridoma antibody and is one of antibody 5F9, 5H3, 6H8, 8C2, 1OC1O, 10D3 and 1D3. In an embodiment, the anti-GCC antibody molecule is derived from antibody 5F9, 5H3, 6H8, 8C2, 1OC10, 10D3, or 1D3. In an embodiment, the anti-GCC antibody molecule is produced by hybridoma 5F9 (PTA-8132). [0891 In an embodiment the anti-GCC antibody molecule is a selected lymphocyte antibody and is one of antibody Abx-12, Abx-020, Abx-106, Abx-198, Abx 221, Abx-229, Abx-338, and Abx-393. In an embodiment, the anti-GCC antibody molecule is derived from antibody Abx-12, Abx-020, Abx-106, Abx-198, Abx-221, Abx 229, Abx-338, and Abx-393. [0901 In an embodiment the anti-GCC antibody molecule is a marine antibody and is one of antibody mAb 3G], mAb 8E12, mAblOB8, and mAb 8F1. Inan 28 WO 2011/050242 PCT/US2010/053686 embodiment, the anti-GCC antibody molecule is derived from antibody mAb 3G1, mAb 8E12, and mAb 8F1. [0911 In an embodiment an anti-GCC antibody molecule will have an affinity for GCC, e.g., as measured by direct binding or competition binding assays, in a range described herein. In an embodiment the anti-GCC antibody molecule has a K of less than 1x10- 6 M, less than 1x10 7 M, less than x10- 8 M, less than 1x10- 9 M, less than lx10-1 M, less than 1x10-" M, less than lx10- 2 M, or less than 1x10-" M. In an embodiment the antibody molecule is an IgG, or antigen-binding fragment thereof, and has a Ks of less than 1x10- 6 M, less than lxl0 7 M, less'than 1x10 8 M, or less than 1x10 9 M. In an embodiment, an anti-GCC antibody molecule, e.g., a 5F9 antibody or antibody derived therefrom has a Kd of about 80 to about 200 pM, preferably about 100 to about 150 pM or about 120 pM. In an embodiment, an anti-GCC antibody molecule, e.g., a 5F9 antibody or antibody derived therefrom has a k, of about 0.9 to about 1.25 x10 5 M-s-1, preferably about 1.1 x10 5 Ms. In an embodiment the antibody molecule is an ScFv and has a Kd of less than lxI 0- 6 M, less than lx104 M, less than lx10- M, less than 1x10 9 M, less than 1x10- M, less than 1x10-" M, less than lx1 2 M, or less than Ix10- 3 M. [092] In embodiments, the antibody molecules are not immunoconjugates, i.e., are "naked" and in embodiments cause a cellular reaction upon binding to GCC, In related embodiments, the cellular reaction is performed by the GCC-expressing cell to which the antibody binds. Such a cellular reaction can be signal transduction mediated by GCC, e.g., if the antibody molecule is an agonist of GCC (see, e.g., US Patent Application publication no. US20040258687. In other embodiments, the cellular reaction is performed by a second cell, e.g., an immune effector cell (e.g., a natural killer cell) which recognizes the antibody molecule bound to GCC on the first cell. In some embodiments, surveillance molecules, e.g., complement molecules, contact the GCC-bound antibody molecule prior to the cellular reaction. The cellular reactions in these embodiments can cause death of the GCC-expressing cell. [093] In further embodiments, antibody molecules which are immunoconjugates can both cause a cellular reaction upon binding to GCC and internalize to deliver an agent to the GCC-expressing cell to which it binds. [094] In some embodiments, an anti-GCC antibody molecule of the invention can block ligand binding to GCC. 29 WO 2011/050242 PCT/US2010/053686 [0951 In an embodiment, the anti-GCC antibody molecule fails to show substantial cross reaction with one or both of rat GCC and mouse GCC. [096] In an embodiment, the antibody molecule is not GCC:B 10, GCC:4D7 or GCC:C8. In another embodiment, an anti-GCC antibody molecule does not bind an intracellular domain of GCC, about amino acid residue 455 to 1073 of SEQ ID NO:228. For example, in this embodiment, an anti-GCC antibody molecule does not bind the kinase homology domain or the guanylyl cyclase domain of GCC. [0971 The naturally occurring mammalian antibody structural unit is typified by a tetramer. Each tetramer is composed of two pairs of polypeptide chains, each pair having one "light" (about 25 kDa) and one "heavy" chain (about 50-70 kDa). The amino-terminal portion of each chain includes a variable region of about 100 to 110 or more amino acids primarily responsible for antigen recognition. The carboxy-terminal portion of each chain defines a constant region primarily responsible for effector function. Human light chains can be classified as kappa and lambda light chains. Heavy chains can be classified as mu, delta, gamma, alpha, or epsilon, and define the antibody's isotype as IgM, IgD, IgG, IgA, and IgE, respectively. Within light and heavy chains, the variable and constant regions are joined by a "J" region of about 12 or more amino acids, with the heavy chain also including a "D" region of about 10 more amino acids. See generally, Fundamental Immunology Ch. 7 (Paul, W., ed., 2nd ed. Raven Press, N.Y. (1989)). The variable regions of each light/heavy chain pair form the antibody binding site. Preferred isotypes for the anti-GCC antibody molecules are IgG immunoglobulins, which can be classified into four subclasses, IgGi, IgG2, IgG3 and IgG4, having different gamma heavy chains. Most therapeutic antibodies are human, chimeric, or humanized antibodies of the IgGi type. In a particular embodiment, the anti-GCC antibody molecule has the IgG1 isotype. [098] The variable regions of each heavy and light chain pair form the antigen binding site. Thus, an intact IgG antibody has two binding sites which are the same. However, bifunctional or bispecific antibodies are artificial hybrid constructs which have two different heavy/light chain pairs, resulting in two different binding sites. [099] The chains all exhibit the same general structure of relatively conserved framework regions (FR) joined by three hypervariable regions, also called complementarity determining regions or CDRs. The CDRs from the two chains of each pair are aligned by the framework regions, enabling binding to a specific epitope. Frorn N 30 WO 2011/050242 PCT/US2010/053686 terminal to C-terminal, both light and heavy chains comprise the domains FRI, CDRJ, FR2, CDR2, FR3, CDR3 and FR4. The assignment of amino acids to each domain is in accordance with the definitions of Kabat Sequences of Proteins of Immunological Interest (National Institutes of Health, Bethesda, Md. (1987 and 1991)), or Chothia & Lesk J. Mol. Biol. 196:901-917 (1987); Chothia et al. Nature 342:878-883 (1989). As used herein, CDRs are referred to for each of the heavy (HCDRI, HCDR2, HCDR3) and light (LCDR1, LCDR2, LCDR3) chains. [0100] An anti-GCC antibody molecule can comprise all, or an antigen binding subset of the CDRs, of one or both, the heavy and light chain, of one of the above referenced human hybridoma, selected lymphocyte, or murine antibodies. Amino acid sequences of human hybridoma, selected lymphocyte, and murine antibody portions, including variable regions and CDRs, can be found in Table 3 and Table 5. [01011 Thus, in an embodiment the antibody molecule includes one or both of: (a) one, two, three, or an antigen binding number of, light chain CDRs (LCDR1, LCDR2 and/or LCDR3) of one of the above-referenced human hybridoma, selected lymphocyte, or murine antibodies. In embodiments the CDR(s) may comprise an amino acid sequence of one or more or all of LCDRI-3 as follows: LCDR1, or modified LCDR1 wherein one to seven amino acids are conservatively substituted) LCDR2, or modified LCDR2 wherein one or two amino acids are conservatively substituted); or LCDR3, or modified LCDR3 wherein one or two amino acids are conservatively substituted; and (b) one, two, three, or an antigen binding number of, heavy chain CDRs (HCDR1, HCDR2 and/or HCDR3) of one of the above-referenced human hybridoma, selected lymphocyte, or murine antibodies. In embodiments the CDR(s) may comprise an amino acid sequence of one or more or all of HCDR1-3 as follows: HCDR1, or modified HCDR1 wherein one or two amino acids are conservatively substituted; HCDR2, or modified HCDR2 wherein one to four amino acids are conservatively substituted; or HCDR3, or modified HCDR3 wherein one or two amino acids are conservatively substituted. [01021 Useful immunogens for production of anti-GCC antibodies include GCC e.g., human GCC-expressing cells (e.g., a tumor cell line, e.g., T84 cells, or fresh or frozen colon tumor cells, recombinant cells expressing GCC); membrane fractions of GCC 31 WO 2011/050242 PCT/US2010/053686 expressing cells (e.g., a colon tumor cell line, e.g., T84 cells, or fresh or frozen colonic tumor cells, recombinant cells expressing GCC, e.g., HT-29-GCC#2 cells, which express full-length GCC, or a portion thereof, e.g., CHO GCC #27 cells which express a portion comprising the GCC extracellular domain, e.g., SEQ ID NO:318); isolated or purified GCC, e.g., human GCC protein (e.g., biochemically isolated GCC, e.g., isolated from gastrointestinal tumor cells or recombinant cells expressing GCC or a variant thereof), or a portion thereof (e.g., the extracellular domain of GCC, the kinase homology domain of GCC or the guanylyl cyclase catalytic domain of GCC or peptide corresponding to a portion thereof, e.g., comprising at least about 8, 10, 12, 14, 16, 20, 24, 28 or 32 amino acid residues of SEQ ID NO:228); or an immunogen comprising SEQ ID NO:229 or comprising a mature portion thereof without the signal sequence (i.e., without amino acid residues I to about 21 or 23 of SEQ ID NO:229), e.g., the mature TOK107-hIgG protein, SEQ ID NO:317. [0103] Immunogens can be fused to heterologous sequences to aid in biochemical manipulation, purification, immunization or antibody titer measurement. Such immunogens can comprise a portion of GCC, e.g., the extracellular domain, and a portion comprising a non-GCC polypeptide. Many options exist for constructing a fusion protein for ease of purification or immobilization onto a solid support, e.g., an affinity column or a microtiter plate or other suitable assay substrate/chip. For example, a fusion moiety can add a domain, e.g., glutathione-S-transferase/kinase (GST), which can bind glutathione; an Fc region of an immunoglobulin, which can bind to protein A or protein G; amino acid residues, e.g., two, three, four, five, preferably six histidine residues which can bind nickel or cobalt on an affinity column; an epitope tag, e.g., a portion of c-myc oncogene (myc tag), a FLAG tag (U.S. Patent No. 4,703,004), a hemagglutinin (HA) tag, a T7 gene 10 tag, a V5 tag, an HSV tag, or a VSV-G tag which can bind a tag-specific antibody; or a cofactor, e.g., biotin, which can bind streptavidin. [0104] Immunogens which comprise the Fe portion of an immunoglobulin can hold the GCC, either in solution or attached to a cell, in a configuration which allows structural access to GCC epitopes by the host immune surveillance components for efficient antibody generation. Because immunoglobulin heavy chains comprising the Fc regions associate into dimers through interchain disulfide bonds, immunogens resulting from fusion with Fc regions are dimers. Valency of fusion proteins can reflect the type of immunoglobulin contributing an Fc region. For example, fusions with IgG proteins can be 32 WO 2011/050242 PCT/US2010/053686 dimers, IgA fusions can make tetrameric immunogens, and IgM fusions can make decameric immunogens, the latter two is facilitated with co-transfection of the J chain. An exemplary immunoglobulin for an FEc fusion protein is IgGI. The portion used typically has the IgG1 hinge, CH2 and CH3 domains encoded by a single exon. Because this exon also has a portion of the CHI region, which has a cysteine oriented to disulfide bond with a cysteine from the light chain, a useful modification is to mutate the CHI cysteine, e.g., to a serine, to ensure there is no unpaired cysteine in the fusion protein. Such a mutation also increases flexibility of the hinge. [0105] An FEc portion derived from a non-host species, e.g., human Ig Fc region, for fusing to an immunogen for immunization in a host species, e.g., mouse, rat, rabbit, goat, acts as an adjuvant. This adjuvant function can trigger specific antibodies against both FEc and GCC epitopes. Fc-reactive antibodies can be identified and discarded during screening. The Fc portion can have a wild type sequence or a sequence which is mutated to modify effector function. For example, a mutated constant region (variant) can be incorporated into a fusion protein to minimize binding to Fc receptors and/or ability to fix complement. (see e.g. Winter et al.,GB 2,209,757 B; Morrison et al., WO 89/07142; Morgan et al., WO 94/29351). In a preferred example, lysine 235 and glycine 237, numbered according to Fc region standards, are mutated, e.g., to alanine. An immunogen/fusion protein with Fc-mutated IgG can have reduced interaction with Fc receptors in the host. A preferred soluble immunogen fusion protein (after maturation to cleave the signal peptide and secretion) is TOK-107-hIgG (alt. name hGCC-ECD/hIgG1 Fc), which consists of amino acid residues 24 to 430 of SEQ ID NO:228 fused to mutated human IgG1 immunoglobulin Fc (SEQ ID NO:317). [0106] To prepare a cell-expressed immunogen, the immunoglobulin portion can be structured to mimic an immunoglobulin portion of the B cell receptor. For example, the immunoglobulin FEc region can be further fused to a polypeptide comprising a transmembrane region from an immune receptor, such as Fcy receptors, Fca receptors, Fca/p receptor or Fce receptors. Proper orientation of such an FEc receptor cell-bound immunogen with adequate exposure on the cell surface may be improved if the cell expressing the immunogen fusion pmtein further comprises additional components of the antigen receptor complex, e.g., B cell IgM receptor or IgD receptor. Suitable components of the complex include immunoglobulin (Ig) sheath pmteins, such as MB-1 and B29 33 WO 2011/050242 PCTIIJS2010/053686 (CD79A and CD79B; Hombach et al. Eur. J ImmunoL 20:2795-2799 (1990) for IgM receptor), which form a heterodimer. The Ig sheath proteins can be provided endogenously by the transfected cell, e.g., if transfecting a B cell lymphoma cell line; or by co-transfection of the immunogen with sheath proteins, e.g., in a separate vector or in the same vector. Preferred IgG sheath proteins for immunization in mouse are mouse CD79a and CD79b (GenBank Accession Nos. NM_007655 and NM_008339, respectively). A preferred cell-bound immunogen fusion protein (after maturation to cleave the signal peptide and translocation to the cell surface) is the TOKi I product, consisting of the TOK-107hlgG (hGCC-ECD/hIgG1 Fe) fused to the mouse IgG2a (e.g., GenPept Accession No. AA1B5966 1) transmembrane and intracellular domains (SEQ ID NO:318). [01071 Useful epitopes, e.g., reference epitopes, from the GCC molecule, to which the anti-GCC antibody molecules, e.g., monoclonal antibodies, human antibodies or humanized antibodies, as described herein, can bind, can be found on the extracellular portion of GCC. Such GCC epitopes can bind antibody molecules on the surface of cells, e.g., on the cell exterior. [0108] For example, an epitope for an anti-GCC antibody molecule can reside within, or include a residue(s) from, residues 1-50 of SEQ ID NO:228, or a fragment thereof that binds an anti-GCC antibody molecule of the invention, e.g., a 5F9-binding fragment thereof. Such fragments can comprise residues 1-25, 5-30, 10-35, 15-40, 20-45, 25-50, 5-45, 10-40, 15-35, 20-30 or 33-50 of SEQ ID NO:228. In some embodiments, an epitope for an anti-GCC antibody molecule, e.g., a 5F9 antibody, is a conformational epitope further comprising one or more additional amino acid residues in the GCC amino acid sequence beyond residue 50, i.e., selected from about residue 50 to 1073 of SEQ ID NO:228. [0109] In another example, an epitope for an anti-GCC antibody molecule can reside within, or include a residue(s) from, SEQ ID NO:225, or residues 271-300 of SEQ ID NO:228, or a fragment thereof that binds an anti-GCC antibody molecule of the invention, e.g., an ABX-198-, a 3G1-, an 8F1-, or a 10B8-binding fragment thereof. Such fragments can comprise residues 281-290 of SEQ ID NO:228, or residues 281-290 of SEQ ID NO:228 wherein residue 281 is leucine, or residues 281-300 or residues 271-290 of SEQ ID NO:228. In some embodiments, an epitope fbr an anti-GCC antibody molecule, e.g., an ABX-198-, a 3G1-, an 8F1-, or a 10B8 antibody, is a conformational epitope 34 WO 2011/050242 PCT/US20101053686 further comprising one or more additional amino acid residues, i.e., non-SEQ ID NO:225 residues in the GCC amino acid sequence e.g., selected from about residue I to 270 and/or about 301 to 1073 of SEQ ID NO:228. [01101 In another example, an epitope for the anti-GCC antibody molecule can reside within, or include a residue(s) from SEQ ID NO:226, or residues 351-375 of SEQ ID NO:228, or a fragment thereof that binds an anti-GCC antibody molecule of the invention, e.g., an ABX-012-, ABX-338-, or ABX-106-binding fragment thereof. Such fragments can comprise 356-370 of SEQ ID NO:228, or residues 351-370 of SEQ ID NO:228, or residues 356-375 of SEQ ID NO:228, In some embodiments, an epitope for an anti-GCC antibody molecule, e.g., an ABX-012-, an ABX-338-, or an ABX-106 antibody, is a conformational epitope further comprising one or more additional amino acid residues, i.e., non-SEQ ID NO:226 residues in the GCC amino acid sequence e.g., selected from about residue 1 to 350 and/or about 376 to 1073 of SEQ ID NO:228. [0111] Antibodies raised against such epitopes or the extracellular domain, e.g., epitopes that reside within, or include a residue(s) from amino acid residues 24 to 420 of SEQ ID NO:228, or a reference portion thereof, e.g., residues 24 to 75, 75 to 150, 150 to 225, 225 to 300, 300 to 375 or 375 to 420 of GCC, or antibody molecules derived therefrom, can be useful as therapeutic or diagnostic antibodies, as described herein. [0112] In an embodiment, the anti-GCC antibody molecule has one or more of the following properties: a) it competes for binding, e.g., binding to cell surface GCC or purified GCC, with one of the above-referenced anti-GCC antibody molecules summarized in Tables 1 and 2 e.g., human hybridoma antibodies (e.g., 5F9), selected lymphocyte antibodies (e.g., Abx-229), or urine antibodies (e.g., 3G1); b) it binds to the same, or substantially the same, epitope on GCC as one of the above-referenced anti-GCC antibody molecules summarized in Tables 1 and 2, e.g., human hybridoma antibodies (e.g., 5F9), selected lymphocyte antibodies (e.g., Abx-229), or murine antibodies (e.g., 3G1). In an embodiment, the antibody binds the same epitope, as determined by one or more of a peptide array assay or by binding to truncation mutants, chimeras or point mutants expressed on the cell surface or membrane preparations, e.g., as those assays are described herein; 35 WO 2011/050242 PCTIUS201O/053686 c) it binds to an epitope which has at least 1, 2, 3, 4, 5, 8, 10, 15 or 20 contiguous amino acid residues in common with the epitope of one of the above referenced anti-GCC antibody molecules summarized in Tables 1 and 2, e.g., human hybridoma antibodies (e.g., 5F9), selected lymphocyte antibodies (e.g., Abx-229) or murine antibodies (e.g., 3G1); d) it binds a region of human GCC that is bound by an anti-GCC antibody of the invention, wherein the region e.g., an extracellular or cytoplasmic region, is 10-15, 10-20, 20-30, or 20-40 residues in length, and binding is determined, e.g., by binding to truncation mutants; In an embodiment the anti-GCC antibody molecule binds the extracellular region of human GCC. In an embodiment an anti-GCC antibody molecule can bind the human GCC portion of the extracellular domain defined by amino acid residues 24 to 420 of SEQ ID NO:228. In an embodiment an anti-GCC antibody molecule can bind the guanylate cyclase signature site at amino acid residues 931 to 954 of SEQ ID NO:228; or e) it binds to a reference epitope described herein. [0113] In an embodiment the anti-GCC antibody molecule binds the GCC sequence ILVDLFNDQYFEDNVTAPDYMKNVLVLTLS (SEQ ID NO:225). [0114] In an embodiment the anti-GCC antibody molecule binds the GCC sequence FAHAFRNLTFEGYDGPVTLDDWGDV (SEQ ID NO:226). [01151 In an embodiment the antibody molecule binds a conformational epitope. In other embodiments an antibody molecule binds a linear epitope. [0116] The anti-GCC antibody molecules can be polyclonal antibodies, monoclonal antibodies, monospecific antibodies, chimeric antibodies (See U.S. Pat. No. 6,020,153) or human or humanized antibodies or antibody fragments or derivatives thereof. Synthetic and genetically engineered variants (See U.S. Pat. No. 6,331,415) of any of the foregoing are also contemplated by the present invention. Monoclonal antibodies can be produced by a variety of techniques, including conventional murine monoclonal antibody methodology e.g., the standard somatic cell hybridization technique of Kohler and Milstein, Nature 256: 495 (1975). See generally, Harlow, E. and Lane, D. (1988) Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 36 WO 2011/050242 PCTIUS2010/053686 [01171 Immunization with protein, e.g., GCC or a soluble portion, or fusion protein comprising a portion of GCC (e.g., TOK107-hIg), or cells or membrane fractions therefrom, e.g., cells expressing surface-exposed GCC or a portion thereof (e.g., the pLKTOK4 product or the pLKTOK1 11 product), can be performed with the immunogen prepared for injection in a manner to induce a response, e.g., with adjuvant, e.g., complete Freund's adjuvant. Other suitable adjuvants include, TITERMAX GOLD@ adjuvant (CYTRX Corporation, Los Angeles, CA) and alum. Small peptide irnmunogens can be linked to a larger molecule, such as keyhole limpet hemocyanin. Mice can be injected in a number of manners, e.g., subcutaneous, intravenous or intramuscular at a number of sites, e.g., in the peritoneum (i.p.), base of the tail, or foot pad, or a combination of sites, e.g., iP and base of tail (BIP). Booster injections can include the same or a different immunogen and can additionally include adjuvant, e.g., incomplete Freund's adjuvant Immunization with DNA, e.g., DNA encoding GCC or a portion thereof or fusion protein comprising GCC or a portion thereof (e.g., encoding TOK107-hIg) can be injected using gene gun technology. For example, DNA is loaded onto microscopic gold particles and injected into mice at frequent intervals over a brief period. [0118] Generally, where a monoclonal antibody is desired, a hybridoma is produced by fusing a suitable cell from an immortal cell line (e.g., a myeloma cell line such as SP2/0, P3X63Ag8.653 or a heteromyeloma) with antibody-producing cells. Antibody-producing cells can be obtained from the peripheral blood or, preferably the spleen or lymph nodes, of humans, human-antibody transgenic animals or other suitable animals immunized with the antigen of interest. Cells that produce antibodies of human origin (e.g., a human antibody) can be produced using suitable methods, for example, fusion of a human antibody-producing cell and a heteromyeloma or trioma, or immortalization of an activated human B cell via infection with Epstein Barr virus. (See, e.g., U.S. Patent No. 6,197,582 (Trakht); Niedbala et al., Hybridoma, 17:299-304 (1998); Zanella et al., JImmunolMethods, 156:205-215 (1992); Gustafsson et al., Hum Antibodies Hybridomas, 2:26-32 (1991).) The fused or immortalized antibody-producing cells (hybridomas) can be isolated using selective culture conditions, and cloned by limiting dilution. Cells which produce antibodies with the desired specificity can be identified using a suitable assay (e.g., ELISA (e.g., with immunogen, e.g., TOK107-hIgG, immobilized on the microtiter well) or by FACS on a cell expressing GCC or a portion thereof, e.g., a cell expressing the pLKTOK 111 product). For example, if the GCC 37 WO 2011/050242 PCT/US2010/053686 immunogen comprises a fusion moiety that is an affinity reagent, this moiety can allow the fusion protein comprising GCC or a portion thereof to be bound to a matrix, e.g., protein G-coated, streptavidin-coated, glutathione-derivatized or antibody-coated microtitre plates or assay chips, which are then combined with the immune serum or conditioned medium from a hybridoma or antibody-expressing recombinant cell, and the mixture incubated under conditions conducive to complex formation (e.g., at physiological conditions for salt and pH). Following incubation, the microtitre plate wells or chip cells are washed to remove any unbound components and binding by anti-GCC antibody is measured. [0119] In embodiments, for therapeutic applications, the antibodies of the present invention are human or humanized antibodies. The advantage of human or humanized antibodies is that they potentially decrease or eliminate the immunogenicity of the antibody in a host recipient, thereby permitting an increase in the bioavailability and a reduction in the possibility of adverse immune reaction, thus potentially enabling multiple antibody administrations. [0120] Modified antibodies include humanized, chimeric or CDR-grafted antibodies. Human anti-mouse antibody (HAMA) responses have led to development of chimeric or otherwise humanized antibodies. While chimeric antibodies have a human constant region and a murine variable region, it is expected that certain human anti chimeric antibody (HACA) responses will be observed, particularly in chronic or multi dose utilizations of the antibody. The presence of such murine or rat derived proteins can lead to the rapid clearance of the antibodies or can lead to the generation of an immune response against the antibody by a patient. In order to avoid the utilization of murine or rat derived antibodies, humanized antibodies where sequences are introduced to an antibody sequence to make it closer to human antibody sequence, or fully human antibodies generated by the introduction of human antibody function into a rodent have been developed so that the rodent would produce antibodies having fully human sequences. Human antibodies avoid certain of the problems associated with antibodies that possess murine, rabbit, or rat variable and/or constant regions. Human Antibodies [01211 Fully human antibody molecules can minimize the immunogenic and allergic responses intrinsic to mouse or mouse-derivatized mAbs and thus to increase the 38 WO 2011/050242 PCT/IUS2010/053686 efficacy and safety of the administered antibodies. The use of fully human antibody molecules can provide a substantial advantage in the treatment of chronic and recurring human diseases, such as inflammation, autoimmunity, and cancer, which require repeated antibody administrations. Also, human antibody molecules can be produced using genetically engineered strains of animals in which the antibody gene expression of the animal is suppressed and functionally replaced with human antibody molecule gene expression. [01221 Methods for making human antibodies are known in the art. One method for making human antibodies employs the use of transgenic animals, such as a transgenic mouse. These transgenic animals contain a substantial portion of the human antibody producing genome, e.g., a human immunoglobulin locus that can undergo functional rearrangement, inserted into their own genome and the animal's own endogenous antibody production is rendered deficient in the production of antibodies. Methods for making such transgenic animals are known in the art. Such transgenic animals can be made using XENOMOUSE Tm technology or by using a "minilocus" approach. Methods for making XENOMICETM are described in U.S. Pat. Nos. 6,162,963, 6,150,584, 6,114,598 and 6,075,181, which are incorporated herein by reference. Methods for making transgenic animals using the "minilocus" approach are described in U.S. Pat. Nos. 5,545,807, 5,545,806 and 5,625,825; also see International Publication No. W093/12227, which are each incorporated herein by reference. Other transgenic human antibody- producing mice include HUMAB-MOUSE @, KIRIN TC MOUSETm transchromosome mice, KM MOUSE@ (MEDAREX, Princeton, NJ). [01231 Using the human antibody transgenic animal technology, e.g., XENOMOUSE TM technology, human antibodies can be obtained by immunizing a XENOMOUSE TM mouse (Abgenix, Fremont, Calif) with an antigen of interest. The lymphatic cells (such as B-cells) are recovered (e.g., isolated from spleen tissue) from the mice that express antibodies. These recovered cells can be fused with a myeloid-type cell line to prepare immortal hybridoma cell lines, using standard methodology. These hybridoma cell lines can be screened and selected to identify hybridoma cell lines that produce antibodies specific to the antigen of interest. [0124] Human-antibody transgenic animals provide a source of nucleic acids that can be enriched in nucleic acids that encode antibodies having desired properties, such as 39 WO 2011/050242 PCT/US2010/053686 specificity and affinity. For example, nucleic acids encoding antibodies or antibody variable regions can be isolated from human-antibody transgenic mice that have been immunized with a GCC protein or variant or portion thereof. The isolated nucleic acids or portions thereof (e.g., portions encoding variable regions, CDRs, framework regions) can be expressed using any suitable method (e.g., phage display) to produce a library of antibodies or antigen-binding fragments of antibodies (e.g., single chain antigen-binding fragments, double chain antigen-binding fragments) that is enriched for antibodies or antigen-binding fragments that bind a GCC protein. Such a library can exhibit enhanced diversity (e.g., combinatorial diversity through pairing of heavy chain variable regions and light chain variable regions) relative to the repertoire of antibodies produced in the immunized human-antibody transgenic animal. The library can be screened using any suitable assay (e.g., a GCC protein binding assay) to identify antibodies or antigen-binding fragments having desired properties (e.g., specificity, affinity). The nucleic acids encoding antibody or antigen-binding fragments having desired properties can be recovered using any suitable method. (See, e.g., U.S. Patent No. 5,871,907 (Winter et al.) and U.S. Patent No. 6,057,098 (Buechler et al.).) [0125] Alternatively, the antibodies can be expressed in cell lines other than hybridoma cell lines. More specifically, sequences encoding particular antibodies can be cloned from cells producing the antibodies and used for transformation of a suitable mammalian host cell. In a preferred method, spleen and/or lymph node lymphocytes from immunized mice are isolated ftom the mice and plated in plaque assays as described previously infBabcook et al., Proc Natl Acad Sci USA. 93: 7843-8 (1996), which is incorporated herein by reference. Briefly, cells are plated in agar with sheep red blood cells, coated with GCC antigen and cells secreting mAb against the GCC antigen would fix complement and lyse the red blood cells immediately surrounding the mAb producing cells. Cells within the cleared plaques are lifted for sequencing of the immunoglobulin sequences and subcloning into expression vectors. Supernatants from transiently transfected cells containing GCC specific mAb are subsequently screened by ELISA and for binding to cells by flow cytometry. The variable sequences, or a portion thereof of the produced human antibodies comprising CDRs which bind particular epitopes may be utilized for production of modified antibodies. For example, the variable regions of the produced antibodies may be spliced into an expression cassette for ease of transfer of constructs, increased expression of constructs, and/or incorporation of constructs into 40 WO 2011/050242 PCT/US2010/053686 vectors capable of expression of fil length antibodies, see, e.g., US20060147445. In a particular embodiment, the expression cassette comprises the heavy chain constant region of the IgG1 isotype. [01261 The Selected Lymphocyte Antibody Method (SLAM, see U.S. Pat No. 5,627,052, Babcook et al. Proc. Natl. Acad. Sci. U.S.A. 93:7843-7848 (1996)) can also be used to identify cells which can provide the antibody of interest. In SLAM, B-cells are cultured directly, thus bypassing hybridoma technology, which typically captures only small percentage of the antibodies originally generated by a mouse. Using microplate based assays, the B-cells are rapidly assayed over a period of several days. Typically, thousands of antigen-reactive cell-clones are identified, representing thousands of individual antigen-specific, e.g., GCC-specific, monoclonal antibodies. The number of different antigen-reactive monoclonal antibodies identified in a single experiment is typically increased many-fold. After applying additional rapid microplate-based assays to measure and rank antibodies by affinity and function, individual B-cell clones producing extremely high quality antibodies can be selected. In addition, by bypassing the hybridoma generation step, production can move rapidly into a recombinant manufacturing cell line. Individual B cells selected using the technology are isolated and the antibody genes can be directly introduced into a manufacturing cell line. The resulting cell line then can be developed for clinical trial testing over essentially the same timeline as that required for hybridoma cell line development. [0127] Human mAb 5F9 (IgG2, kappa) can be produced by hybridoma 5F9, also referred to as hybridoma 46.5F9.8.2, which was deposited on January 10, 2007, on behalf of Millennium Pharmaceuticals Inc., 40 Landsdowne Street, Cambridge, MA, 02139, USA, at the American Type Culture Collection, 10801 University Boulevard, Manassas, Virginia 20110, U.S.A., under Accession No. PTA-8132. (The deposit was made pursuant to, and in satisfaction of, the requirements of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure.) The invention relates to hybridoma 5F9, to the antibody it produces, antigen-binding fragments thereof, and to nucleic acids encoding the antibody and portions thereof (e.g., heavy chain, heavy chain variable region, light chain, light chain variable region, CDRs). As described herein, hybridoma 5F9 produces an IgG2, kappa antibody. 41 WO 2011/050242 PCT/US2010/053686 Humanization and Display Technologies and Modpications to Antibodies [0128] As discussed above, there are advantages to producing antibodies with reduced immunogenicity. This can be accomplished in connection with techniques of humanization and display techniques using appropriate libraries. It will be appreciated that murine antibodies or antibodies from other species can be humanized or privatized using techniques known in the art. See e.g., Winter and Harris Immunol Today 14:43-46 (1993) and Wright et at Crit. Reviews in Immunol. 12125-168 (1992). The antibody of interest may be engineered by recombinant DNA techniques to substitute the CH1, CH2, CH3, hinge domains, and/or the framework domain with the corresponding human sequence (see WO 92/02190 and U.S. Pat. Nos. 5,530,101, 5,585,089, 5,693,761, 5,693,792, 5,714,350, and 5,777,085). Also, the use of Ig cDNA for construction of chimeric immunoglobulin genes is known in the art (Liu et al Proc NatlAcadSci USA. 84:3439 (1987) and J Immunol. 139:3521 (1987)). nRNA is isolated from a hybridoma or other cell producing the antibody and used to produce cDNA: The cDNA of interest may be amplified by the polymerase chain reaction using specific primers (U.S. Pat. Nos. 4,683,195 and 4,683,202). [0129] Alternatively, phage display technology (see, e.g., McCafferty et al, Nature, 348:552- 553 (1990)) can be used to produce human antibodies and antibody fragments in vitro, from immunoglobulin variable (V) domain genes, e.g., from repertoires from unimmunized donors. According to this technique, antibody V domain genes are cloned in-frame into either a major or minor coat protein gene of a filamentous bacteriophage, such as M13 or fd, and displayed as functional antibody fragments on the surface of the phage particle. Because the filamentous particle contains a single-stranded DNA copy of the phage genome, selections based on the functional properties of the antibody also result in selection of the gene encoding the antibody exhibiting those properties. Thus, the phage mimics some of the properties of the B cell. Phage display can be performed in a variety of formats; for their review see, e.g., Johnson and Chiswell, Current Opinion in Structural Biology, 3:564-571 (1993). Several sources of V-gene segments can be used for phage display. Clackson et al., Nature, 352:624-628 (1991) isolated a diverse array of anti oxazolone antibodies from a small random combinatorial library of V genes derived from the spleens of immunized mice. A repertoire of V genes from unimmunized human donors can be constructed and antibodies to a diverse array of antigens (including self-antigens) 42 WO 2011/050242 PCT/US2010/053686 can be isolated essentially following the techniques described by Marks et al., J Mol. BioL, 222:581- 597 (1991), or Griffith et al, EMBOJ, 12:725-734 (1993). See, also, U.S. Patent Nos. 5,565,332 and 5,573,905. Display libraries can contain antibodies or antigen binding fragments of antibodies that contain artificial amino acid sequences. For example, the library can contain Fab fragments which contain artificial CDRs (e.g., random amino acid sequences) and human framework regions. (See, for example, U.S. Patent No. 6,300,064 (Knappik, et al.).) [0130] Human antibodies may also be generated by in vitro activated B cells (see U.S. Patent Nos. 5,567,610 and 5,229,275). [0131] The sequences of human constant region genes may be found in Kabat et al. (1991) Sequences ofProteins ofImmunological Interest, N.I.H. publication no. 91-3242. Human C region genes are readily available from known clones. The choice of isotype will be guided by the desired effector functions, such as complement fixation, or activity in antibody-dependent cellular cytotoxicity. Isotypes can be IgG1, IgG2, IgG3 or IgG4. In particular embodiments, antibody molecules of the invention are IgG1 and IgG2. Either of the human light chain constant regions, kappa or lambda, may be used. The chimeric, humanized antibody is then expressed by conventional methods. [0132] In some embodiments, an anti-GCC antibody molecule of the invention can draw antibody-dependent cellular cytotoxicity (ADCC) to a cell expressing GCC, e.g., a tumor cell. Antibodies with the IgG 1 and IgG3 isotypes are useful for eliciting effector function in an antibody-dependent cytotoxic capacity, due to their ability to bind the Fc receptor. Antibodies with the IgG2 and IgG4 isotypes are useful to minimize an ADCC response because of their low ability to bind the Fc receptor. In related embodiments substitutions in the Fc region or changes in the glycosylation composition of an antibody, e.g., by growth in a modified eukaryotic cell line, can be made to enhance the ability of Fc receptors to recognize, bind, and/or mediate cytotoxicity of cells to which anti-GCC antibodies bind (see, e.g., U.S. Pat No. 7,317,091, 5,624,821 and publications including WO 00/42072, Shields, et al. J BioL Chem. 276:6591-6604 (2001), Lazar et al. Proc. Nat. Acad. Sci. U.S.A. 103:4005-4010 (2006), Satoh et al. Expert Opin Biol. Ther. 6:1161-1173 (2006)). In certain embodiments, the antibody or antigen-binding fragment (e.g., antibody of human origin, human antibody) can include amino acid substitutions or replacements that alter or tailor function (e.g., effector function), For example, a constant region of 43 WO 2011/050242 PCT/US20101053686 human origin (e.g., yl constant region, y2 constant region) can be designed to reduce complement activation and/or Fe receptor binding. (See, for example, U.S. Patent Nos. 5,648,260 (Winter et al.), 5,624,821 (Winter et al.) and 5,834,597 (Tso et al.), the entire teachings of which are incorporated herein by reference.) Preferably, the amino acid sequence of a constant region of human origin that contains such amino acid substitutions or replacements is at least about 95% identical over the full length to the amino acid sequence of the unaltered constant region of human origin, more preferably at least about 99% identical over the full length to the amino acid sequence of the unaltered constant region of human origin. [0133] In still another embodiment, effector functions can also be altered by modulating the glycosylation pattern of the antibody. By altering is meant deleting one or more carbohydrate moieties found in the antibody, and/or adding one or more glycosylation sites that are not present in the antibody. For example, antibodies with enhanced ADCC activities with a mature carbohydrate structure that lacks fucose attached to an Fc region of the antibody are described in U.S. Patent Application Publication No. 2003/0157108 (Presta). See also U.S. Patent Application Publication No. 2004/0093621 (Kyowa Hakko Kogyo Co., Ltd). Glycofi has also developed yeast cell lines capable of producing specific glycoforms of antibodies. [0134] Additionally or alternatively, an antibody can be made that has an altered type of glycosylation, such as a hypofucosylated antibody having reduced amounts of fucosyl residues or an antibody having increased bisecting GlcNac structures. Such altered glycosylation patterns have been demonstrated to increase the ADCC ability of antibodies. Such carbohydrate modifications can be accomplished by, for example, expressing the antibody in a host cell with altered glycosylation machinery. Cells with altered glycosylation machinery have been described in the art and can be used as host cells in which are engineered to express recombinant antibodies of the invention to thereby produce an antibody with altered glycosylation. For example, EP 1,176,195 by Hang et al. describes a cell line with a functionally disrupted FUT8 gene, which encodes a fucosyl transferase, such that antibodies expressed in such a cell line exhibit hypofucosylation. PCT Publication WO 03/035 835 by Presta describes a variant CHO cell line, Lec13 cells, with reduced ability to attach fucose to Asn(297)-linked carbohydrates, also resulting in hypofucosylation of antibodies expressed in that host cell (see also Shields, R. L. et al., 2002 J Biot Chem. 277:26733-26740). PCT Publication WO 99/54342 by Umana et al. 44 WO 2011/050242 PCT/US2010/053686 describes cell lines engineered to express glycoprotein-modifying glycosyl transferases (e.g., beta(1,4)-N acetylglucosaminyltransferase III (GnTIII)) such that antibodies expressed in the engineered cell lines exhibit increased bisecting GlcNac structures which results in increased ADCC activity of the antibodies (see also Umana et aL., 1999 Nat. Biotech. 17:176-180). [0135] Humanized antibodies can also be made using a CDR-grafted approach. Techniques of generation of such humanized antibodies are known in the art. Generally, humanized antibodies are produced by obtaining nucleic acid sequences that encode the variable heavy and variable light sequences of an antibody that binds to GCC, identifying the complementary determining region or "CDR" in the variable heavy and variable light sequences and grafting the CDR nucleic acid sequences on to human framework nucleic acid sequences. (See, for example, U.S. Pat. Nos. 4,816,567 and 5,225,539). The location of the CDRs and framework residues can be determined (see, Kabat, E.A., et aL. (1991) Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242, and Chothia, C. et al. J. MoL. BioL 196:901-917 (1987)). Anti-GCC antibody molecules described herein have the CDR amino acid sequences and nucleic acid sequences encoding CDRs listed in Tables 5 and 6. In some embodiments sequences from Tables 5 and 6 can be incorporated into molecules which recognize GCC for use in the therapeutic or diagnostic methods described herein. The human framework that is selected is one that is suitable for in vivo administration, meaning that it does not exhibit immunogenicity. For example, such a determination can be made by prior experience with in vivo usage of such antibodies and studies of amino acid similarities. A suitable framework region can be selected from an antibody of human origin having at least about 65% amino acid sequence identity, and preferably at least about 70%, 80%, 90% or 95% amino acid sequence identity over the length of the framework region within the amino acid sequence of the equivalent portion (e.g., framework region) of the donor antibody, e.g., an anti-GCC antibody molecule (e.g., 3G1). Amino acid sequence identity can be determined using a suitable amino acid sequence alignment algorithm, such as CLUSTAL W, using the default parameters. (Thompson J.D. et aL, Nucleic Acids Res. 22:4673-4680 (1994).) [0136] Once the CDRs and FRs of the cloned antibody that are to be humanized are identified, the amino acid sequences encoding the CDRs are identified and the corresponding nucleic acid sequences grafted on to selected human FRs. This can be done 45 WO 2011/050242 PCTUS2010/053686 using known primers and linkers, the selection of which are known in the art. All of the CDRs of a particular human antibody may be replaced with at least a portion of a non human CDR or only some of the CDRs may be replaced with non-human CDRs. It is only necessary to replace the number of CDRs required for binding of the humanized antibody to a predetermined antigen. After the CDRs are grafted onto selected human FRs, the resulting "humanized" variable heavy and variable light sequences are expressed to produce a humanized Fv or humanized antibody that binds to GCC. Preferably, the CDR grafted (e.g., humanized) antibody binds a GCC protein with an affinity similar to, substantially the same as, or better than that of the donor antibody. Typically, the humanized variable heavy and light sequences are expressed as a fusion protein with human constant domain sequences so an intact antibody that binds to GCC is obtained. However, a humanized Fv antibody can be produced that does not contain the constant sequences. [0137] Also within the scope of the invention are humanized antibodies in which specific amino acids have been substituted, deleted or added. In particular, humanized antibodies can have amino acid substitutions in the framework region, such as to improve binding to the antigen. For example, a selected, small number of acceptor framework residues of the humanized immunoglobulin chain can be replaced by the corresponding donor amino acids. Locations of the substitutions include amino acid residues adjacent to the CDR, or which are capable of interacting with a CDR (see e.g., U.S. Patent Nos. 5,585,089 or 5,859,205). The acceptor framework can be a mature human antibody framework sequence or a consensus sequence. As used herein, the term "consensus sequence" refers to the sequence found most frequently, or devised from the most common residues at each position in a sequence in a region among related family members. A number of human antibody consensus sequences are available, including consensus sequences for the different subgroups of human variable regions (see, Kabat, E.A., et al., Sequences of Proteins ofImmunological Interest, Fifth Edition, U.S. Department of Health and Human Services, U.S. Government Printing Office (1991)). The Kabat database and its applications are freely available on line, e.g. via IgBLAST at the National Center for Biotechnology Information, Bethesda, MD (also see, Johnson, G. and Wu, T.T., Nucleic Acids Research 29:205-206 (2001)). [0138] Other techniques for humanizing antibodies are described in Padlan et al. EP 519596 Al, published on December 23, 1992. 46 WO 2011/050242 PCT/US2010/053686 [0139] The anti-GCC antibody molecule includes other humanized antibodies which may also be modified by specific deletion of human T cell epitopes or "deimmunization" by the methods disclosed in PCT Publication Nos. WO 98/52976 and WO 00/34317, the contents of which are incorporated herein by reference. Briefly, the murine heavy and light chain variable regions of an anti-GCC antibody can be analyzed for peptides that bind to MHC Class II; these peptides represent potential T-cell epitopes. For detection of potential T-cell epitopes, a computer modeling approach termed "peptide threading" can be applied, and in addition a database of human MHC class IH binding peptides can be searched for motifs present in the murine VH and VL sequences, as described in PCT Publication Nos. WO 98/52976 and WO 00/34317. These motifs bind to any of the 18 major MHC class I DR allotypes, and thus constitute potential T cell epitopes. Potential T-cell epitopes detected can be eliminated by substituting small numbers of amino acid residues in the variable regions, or preferably, by single amino acid substitutions. As far as possible, conservative substitutions are made, often but not exclusively, an amino acid common at this position in human germline antibody sequences may be used. Human germiline sequences are disclosed in Tomlinson, I.A. et aL., J. Mol. Biol. 227:776-798(1992); Cook, G. P. et al., Immunol. Today Vol. 16 (5): 237-242(1995); Chothia, D. et al., J Mol. Bio. 227:799-817(1992). The V BASE directory provides a comprehensive directory of human immunoglobulin variable region sequences (compiled by Tomlinson, LA. et al. MRC Centre for Protein Engineering, Cambridge, UK). After the deimmunized VH and VL of an anti-GCC antibody are constructed by mutagenesis of the murine VH and VL genes, the mutagenized variable sequence can, optionally, be fused to a human constant region, e.g., human IgGI or K constant regions. [0140] In other embodiments, reduction of an immunogenic response by a CDR grafted antibody can be achieved by changes, e.g., deletions, substitutions, of amino acid residues in CDRs (Kashmiri et al. Methods 36:25-34 (2005), U.S. Pat. No. 6,818,749, Tan et al. J ImmunoL 169:1119-1125 (2006)). For example, residues at positions involved in contact with the antigen preferably would not be changed. Typically, such residues, the SDRs, are in positions which display high levels of variability among antibodies. Consensus sequences (e.g., SEQ ID NOs:302-307, Table 5) derived, e.g., by the Clustal method (Higgins D. G. et al., Meth. Enzymol. 266:383-402 (1996)), from anti-GCC antibody molecules, e.g., from antibodies described herein, aid in identifying SDRs. In the human anti-GCC antibody molecules described herein, the SDRs are the following, at least 47 WO 2011/050242 PCT/US2010/053686 the first residue or in some embodiments, the first four residues of heavy chain CDR1; at least the N-terminal portion, e.g., the first seven, ten or 13 residues of heavy chain CDR2; nearly all of heavy chain CDR3; the C-terminal portion, e.g., after residue six, eight, or nine of light chain CDR1; about the first, middle and/or last residue of light chain CDR2; and most of light chain CDR3, or at least after residue two or three. Accordingly, to maintain binding to GCC protein after humanization or modification of an anti-GCC antibody molecule, such SDR residues in CDRs of the anti-GCC antibody molecules are less amenable to changes, e.g., from urine residues to human consensus residues than are residues in other residues of the CDRs or the framework regions. Conversely, it can be beneficial to change residues in non-human, e.g., murine CDRs to residues identified as consensus in human CDRs, e.g., CDRs of anti-GCC antibody molecules described herein (e.g., the sequences listed in Table 5). For example, a serine can represent a human residue for the C-terminus of heavy chain CDR1, and/or a tyrosine can represent a human residue for the second and/or third residues of heavy chain CDR1; heavy chain CDR2 can end in S-(L/V)-K-(S/G) (SEQ ID NO:312) to represent a human CDR; to represent a human CDR3, there can be a glycine after four to six residues and/or an aspartate six to nine residues in heavy chain CDR3; light chain CDR1 can begin with (K/R)-(A/S)-SQS (V/L)-(SIL) (SEQ ID NO:313) to represent a human CDR; light chain CDR2 can have a serine in the third residue and/or an arginine in the fifth residue represent a human CDR; and/or light chain CDR3 can have a glutamine in the second residue and/or a tyrosine or serine in the third residue represent a human CDR. [01411 Anti-GCC antibodies that are not intact antibodies are also useful in this invention. Such antibodies may be derived from any of the antibodies described above. Useful antibody molecules of this type include (i) a Fab fragment, a monovalent fragment consisting of the VL, VH, CL and CHI domains; (ii) a F(ab) 2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; (iii) a Fd fragment consisting of the VH and CHI domains; (iv) a Fv fragment consisting of the VL and VH domains of a single arm of an antibody, (v) a dAb fragment (Ward et al., Nature 341:544-546 (1989)), which consists of a VH domain; (vii) a single domain functional heavy chain antibody, which consists of a VHH domain (known as a nanobody) see e.g., Cortez-Retamozo, et al., Cancer Res. 64: 2853-2857(2004), and references cited therein; and (vii) an isolated CDR, e.g., one or more isolated CDRs together with sufficient framework to provide an antigen binding fragment. Furthermore, although the two 48 - WO 2011/050242 PCT/UTS2010/053686 domains of the Fv fragment, VL and VH, are coded for by separate genes, they can be joined, using recombinant methods, by a synthetic linker that enables them to be made as a single protein chain in which the VL and VH regions pair to form monovalent molecules (known as single chain Fv (scFv); see e.g., Bird et aL. Science 242:423-426 (1988); and Huston et al. Proc. Natl. Acad. Sci. USA 85:5879-5883 (1988). Such single chain antibodies are also intended to be encompassed within the term "antigen-binding fragment" of an antibody. These antibody fragments are obtained using conventional techniques known to those with skill in the art, and the fragments are screened for utility in the same manner as are intact antibodies. Antibody fragments, such as Fv, F(ab') 2 and Fab may be prepared by cleavage of the intact protein, e.g. by protease or chemical cleavage. [01421 In embodiments, some or all of the CDRs sequences, of one or both the heavy and light chain, can be used in another antibody molecule, e.g., in a CDR-grafted, humanized, or chimeric antibody molecule. [01431 Embodiments include an antibody molecule that comprises sufficient CDRs, e.g., all six CDRs from one of the above-referenced human hybridoma, selected lymphocyte, or murine antibodies to allow binding to cell surface GCC. [0144] In an embodiment the CDRs, e.g., all of the HCDRs, or all of the LCDRs, or all six, are embedded in human or human derived framework region(s). Examples of human framework regions include human germline framework sequences, human germline sequences that have been affinity matured (either in vivo or in vitro), or synthetic human sequences, e.g., consensus sequences. In an embodiment the heavy chain framework is an IgGI or IgG2 framework. In an embodiment the light chain framework is a kappa framework. [0145] In an embodiment the anti-GCC antibody molecule, e.g., a CDR-grafted or humanized antibody molecule, comprises sufficient CDRs, e.g., all six CDRs from one of the antibodies described herein, e.g., sequences listed in Table 5, to allow binding to GCC. (Exemplary nucleic acid sequences which can encode the CDR amino acid sequences listed in Table 5, are provided, in Table 6 herein). In particular embodiments, an anti-GCC antibody molecule can comprise CDRs from 5F9 or Abx-229. [0146] Antibody fragments for in vivo therapeutic or diagnostic use can benefit from modifications which improve their serum half lives. Suitable organic moieties intended to increase the in vivo serum half-life of the antibody can include one, two or 49 WO 2011/050242 PCT/US2010/053686 more linear or branched moiety selected from a hydrophilic polymeric group (e.g., a linear or a branched polymer (e.g., a polyalkane glycol such as polyethylene glycol, monomethoxy-polyethylene glycol and the like), a carbohydrate (e.g., a dextran, a cellulose, a polysaccharide and the like), a polymer of a hydrophilic amino acid (e.g., polylysine, polyaspartate and the like), a polyalkane oxide and polyvinyl pyrrolidone), a fatty acid group (e.g., a mono-carboxylic acid or a di-carboxylic acid), a fatty acid ester group, a lipid group (e.g., diacylglycerol group, sphingolipid group (e.g., ceramidyl)) or a phospholipid group (e.g., phosphatidyl ethanolamine group). Preferably, the organic moiety is bound to a predetermined site where the organic moiety does not impair the function (e.g., decrease the antigen binding affinity) of the resulting immunoconjugate compared to the non-conjugated antibody moiety. The organic moiety can have a molecular weight of about 500 Da to about 50,000 Da, preferably about 2000, 5000, 10,000 or 20,000 Da. Examples and methods for modifying polypeptides, e.g., antibodies, with organic moieties can be found, for example, in U.S. Patent Nos. 4,179,337 and 5,612,460, PCT Publication Nos. WO 95/06058 and WO 00/26256, and U.S. Patent Application Publication No. 20030026805. [0147] An anti-GCC antibody molecule can comprise all, or an antigen binding fragment of the variable region, of one or both, the heavy and light chain, of one of the above-referenced human hybridoma, selected lymphocyte, or murine antibodies. [0148] In an embodiment the light chain amino acid sequence of(a) can differ from one of the reference amino acid sequence(s) referred to in (a)(i-ii) by as many as 1, 2, 3, 4, 5, 10, or 15 residues. In embodiments the differences are conservative substitutions. In embodiments, the differences are in the framework regions. In an embodiment the heavy chain amino acid sequence of(b) can differ from one of the reference amino acid sequence(s) referred to in (b)(i-ii) by as many as 1, 2, 3, 4, 5, 10, or 15 residues. In embodiments the differences are conservative substitutions. In embodiments the differences are in the framework regions. [0149] In an embodiment the anti-GCC antibody molecule comprises one or both of: (a) a light chain amino acid sequence of all, or an antigen binding fragment of, either, (i) a light chain variable region amino acid sequence from Table 3, e.g., SEQ ID 50 WO 2011/050242 PCT/US2010/053686 NO:20, or (ii) a light chain variable region amino acid encoded by a nucleotide sequence from Table 4, e.g., SEQ ID NO:19; and (b) a heavy chain amino acid sequence of all, or an antigen binding fragment of, either (i) a heavy chain variable region amino acid sequence from Table 3, e.g., SEQ ID NO:18, or (ii) a heavy chain amino acid sequence encoded by a nucleotide sequence from Table 4, e.g., SEQ ID NO:17. [0150] In an embodiment the anti-GCC antibody molecule comprises one or both of: a) a light chain variable region, or an antigen binding fragment thereof, having at least 85, 90, 95, 97 or 99 % homology with the light chain variable region of an anti-GCC antibody molecule of the invention, e.g., one of the above-referenced human hybridoma, selected lymphocyte, or murine antibodies; and (b) a heavy chain variable region, or an antigen binding fragment thereof, having at least 85, 90, 95, 97 or 99 % homology with the heavy chain variable region of an anti-GCC antibody molecule of the invention, e.g., one of the above-referenced human hybridoma, selected lymphocyte, or murine antibodies. [0151] Amino acid sequences of the variable regions of human hybridoma, selected lymphocyte, and murine antibodies can be found in Table 3. [01521 In an embodiment, the anti-GCC antibody molecule is a 5F9 antibody molecule and includes one or both of: a) all or a fragment of the heavy chain constant region from SEQ ID NO: 231; and b) all or a fragment of the light chain constant region from SEQ ID NO: 233. [0153] In another embodiment, the anti-GCC antibody molecule is an Abx-229 antibody molecule and includes one or both of: a) all or a GCC-binding fragment of the heavy chain variable region from SEQ ID NO: 46; and b) all or a GCC-binding fragment of the light chain variable region from SEQ ID NO: 48. [0154] In one approach, consensus sequences encoding the heavy and light chain J regions may be used to design oligonucleotides for use as primers to introduce useful restriction sites into the J region for subsequent linkage of V region segments to human C region segments. C region cDNA can be modified by site directed mutagenesis to place a restriction site at the analogous position in the human sequence. 51 WO 2011/050242 PCT/US2010/053686 [0155] Expression vectors include plasmids, retroviruses, cosmids, YACs, EBV derived episomes, and the like. A convenient vector is one that encodes a functionally complete human CH or CL immunoglobulin sequence, with appropriate restriction sites engineered so that any VH or VL sequence can be easily inserted and expressed. In such vectors, splicing usually occurs between the splice donor site in the inserted J region and the splice acceptor site preceding the human C region, and also at the splice regions that occur within the human CH exons. Suitable expression vectors can contain a number of components, for example, an origin of replication, a selectable marker gene, one or more expression control elements, such as a transcription control element (e.g., promoter, enhancer, terminator) and/or one or more translation signals, a signal sequence or leader sequence, and the like. Polyadenylation and transcription termination occur at native chromosomal sites downstream of the coding regions. The resulting chimeric antibody may be joined to any strong promoter. Examples of suitable vectors that can be used include those that are suitable for mammalian hosts and based on viral replication systems, such as simian virus 40 (SV40), Rous sarcoma virus (RSV), adenovirus 2, bovine papilloma virus (BPV), papovavirus BK mutant (BKV), or mouse and human cytomegalovirus (CMV), and moloney murine leukemia virus (MMLV), native Ig promoters, etc. A variety of suitable vectors are known in the art, including vectors which are maintained in single copy or multiple copies, or which become integrated into the host cell chromosome, e.g., via LTRs, or via artificial chromosomes engineered with multiple integration sites (Lindenbaun et al. Nucleic Acids Res. 32:e172 (2004), Kennard et al. Biotechnol. Bioeng. Online May 20, 2009). Additional examples of suitable vectors are listed in a later section. [01561 Thus, the invention provides an expression vector comprising a nucleic acid encoding an antibody, antigen-binding fragment of an antibody (e.g., a human, humanized, chimeric antibody or antigen-binding fragment of any of the foregoing), antibody chain (e.g., heavy chain, light chain) or antigen-binding portion of an antibody chain that binds a GCC protein. [0157] Expression in eukaryotic host cells is useful because such cells are more likely than prokaryotic cells to assemble and secrete a properly folded and immunologically active antibody. However, any antibody produced that is inactive due to improper folding may be renaturable according to known methods (Kim and Baldwin, "Specific Intermediates in the Folding Reactions of Small Proteins and the Mechanism of 52 WO 2011/050242 PCT/US2010/053686 Protein Folding", Ann. Rev. Biochem. 51, pp. 459-89 (1982)). It is possible that the host cells will produce portions of intact antibodies, such as light chain dimers or heavy chain dimers, which also are antibody homologs according to the present invention. [01581 Further, as described elsewhere herein, human antibodies or antibodies from other species can be generated through display-type technologies, including, without limitation, phage display, retroviral display, ribosomal display, and other techniques, using techniques well known in the art and the resulting molecules can be subjected to additional maturation, such as affinity maturation, as such techniques are known in the art. Winter and Harris Immunol Today 14:43-46 (1993) and Wright et al. Crit. Reviews in Immunol. 12125-168 (1992), Hanes and Plucthau PNAS USA 94:4937-4942 (1997) (ribosomal display), Parmley and Smith Gene 73:305-318 (1988) (phage display), Scott TIBS 17:241 245 (1992), Cwirla et aL. Proc Natl Acad Sci USA 87:6378-6382 (1990), Russel et al. NucL. Acids Research 21:1081-1085 (1993), Hoganboom et al. Immunol. Reviews 130:43-68 (1992), Chiswell and McCafferty TIBTECH 10:80-84 (1992), and U.S. Pat. No. 5,733,743. If display technologies are utilized to produce antibodies that are not human, such antibodies can be humanized as described above. [01591 It will be appreciated that antibodies that are generated need not initially possess a particular desired isotype but, rather, the antibody as generated can possess any isotype. For example, the antibody produced by the 5F9 hybridoma (ATCC deposit no. PTA-8132) has the IgG2 isotype. The isotype of the antibody can be switched thereafter, e.g., to IgG1 or IgG3 to elicit an ADCC response when the antibody binds GCC on a cell, using conventional techniques that are known in the art. Such techniques include the use of direct recombinant techniques (see e.g., U.S. Pat. No. 4,816,397), cell-cell fusion techniques (see e.g., U.S. Pat No 5,916,771), among others. In the cell-cell fusion technique, a myeloma or other cell line is prepared that possesses a heavy chain with any desired isotype and another myeloma or other cell line is prepared that possesses the light chain. Such cells can, thereafter, be fused and a cell line expressing an intact antibody can be isolated. [0160] In certain embodiments, the GCC antibody molecule is a human anti-GCC IgG1 antibody. Since such antibodies possess desired binding to the GCC molecule, any one of such antibodies can be readily isotype-switched to generate a human IgG4 isotype, for example, while still possessing the same variable region (which defines the antibody's 53 WO 2011/050242 PCT/TJS2010/053686 specificity and affinity, to a certain extent). Accordingly, as antibody candidates are generated that meet desired "structural" attributes as discussed above, they can generally be provided with at least certain additional fictionala" attributes that are desired through isotype switching. [01611 In an embodiment the variable region or antigen binding fragment thereof can be coupled to a constant region (or fragment thereof) other than the constant region it was generated with, e.g., a constant region (or fragment thereof) from another antibody or to a synthetic constant region (or fragment thereof). In embodiments the constant region is an IgGI or IgG2 constant region (or fragment thereof). Sequence changes can be made in the variable or constant regions to modify effector activity of the antibody molecule. Design and Generation of Other Therapeutics [0162] The antibodies that are produced and characterized herein with respect to GCC provide for the design of other therapeutic modalities including other antibodies, other antagonists, or chemical moieties other than antibodies is facilitated. Such modalities include, without limitation, antibodies having similar binding activity or functionality, advanced antibody therapeutics, such as bispecific antibodies, immunoconjugates, and radiolabeled therapeutics, generation of peptide therapeutics, particularly intrabodies, and small molecules. Furthermore, as discussed above, the effector function of the antibodies of the invention may be changed by isotype switching to an IgG1, IgG2, IgG3, IgG4, IgD, IgAl, IgA2, IgE, or IgM for various therapeutic uses. [01631 In connection with bispecific antibodies, bispecific antibodies can be generated that comprise (i) two antibodies, one with a specificity to GCC and another to a second molecule that are conjugated together, (ii) a single antibody that has one chain specific to GCC and a second chain specific to a second molecule, or (iii) a single chain antibody that has specificity to GCC and the other molecule. Such bispecific antibodies can be generated using techniques that are known. For example, bispecific antibodies may be produced by crosslinking two or more antibodies (of the same type or of different types). Suitable crosslinkers include those that are heterobifunctional, having two distinctly reactive groups separated by an appropriate spacer (e.g., m-maleimidobenzoyl N-hydroxysuccinimide ester) or homobifunctional (e.g., disuccinimidyl suberate). Such linkers are available from Pierce Chemical Company, Rockford, IL. See also, e.g., Fanger et al. Immunomethods 4:72-81 (1994) and Winter and Harris Immunol Today 14:43-46 54 WO 2011/050242 PCT/US2010/053686 (1993) and Wright et al. Cdt. Reviews in ImmunoL 12125-168 (1992) and in connection with (iii) see e.g., Traunecker et al. Int. J. Cancer (Suppl.) 7:51-52 (1992). Songsivilai & Lachmann Clin. Exp. Immunol. 79: 315-321 (1990), Kostelny et al. J. Immunol. 148:1547 1553 (1992). [0164] In addition, "Kappabodies" (Ill. et al. "Design and construction of a hybrid immunoglobulin domain with properties of both heavy and light chain variable regions" Protein Eng 10:949-57 (1997)), "Minibodies" (Martin et al. EMBO J 13:5303-9 (1994), US Patent No. 5,837,821), "Diabodies" (Holliger et al Proc NatlAcadSci USA 90:6444 6448 (1993)), or "Janusins" (Traunecker et al. EMBO J 10:3655.-3659 (1991) and Traunecker et al. Int J Cancer Suppl 7:51-52 (1992)) may also be prepared. Nucleic acid and Polypeptides [01651 In another embodiment, the present invention relates to polynucleotide and polypeptide sequences that encode for or represent the antibody molecules described herein. Such polynucleotides encode for both the variable and constant regions of each of the heavy and light chains, although other combinations are also contemplated by the present invention in accordance with the compositions described herein. The present invention also contemplates oligonucleotide fragments derived from the disclosed polynucleotides and nucleic acid sequences complementary to these polynucleotides. [01661 The polynucleotides can be in the form of RNA or DNA. Polynucleotides in the form of DNA, cDNA, genomic DNA, nucleic acid analogs and synthetic DNA are within the scope of the present invention. The DNA may be double-stranded or single stranded, and if single stranded, may be the coding (sense) strand or non-coding (anti sense) strand. The coding sequence that encodes the polypeptide may be identical to the coding sequence provided herein or may be a different coding sequence which coding sequence, as a result of the redundancy or degeneracy of the genetic code, encodes the same polypeptide as the DNA provided herein. [01671 In embodiments provided, polynucleotides encode at least one heavy chain variable region and at least one light chain variable region of the present invention, e.g., as summarized in Table 4. [0168] The present invention also includes variant polynucleotides containing modifications such as polynucleotide deletions, substitutions or additions, and any 55 WO 2011/050242 PCT/JS2010/053686 polypeptide modification resulting from the variant polynucleotide sequence. A polynucleotide of the present invention may also have a coding sequence that is a variant of the coding sequence provided herein. For example, a variant polynucleotide can have at least 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95% or 97% identity with a polynucleotide listed in Table 4. In embodiments, the variant polynucleotide encodes for an anti-GCC antibody molecule. [0169] The present invention further relates to polypeptides that represent the antibodies of the present invention as well as fragments, analogs and derivatives of such polypeptides. The polypeptides of the present invention may be recombinant polypeptides, naturally produced polypeptides or synthetic polypeptides. The fragment, derivative or analogs of the polypeptides of the present invention may be one in which one or more of the amino acid residues is substituted with a conserved or non-conserved amino acid residue (preferably a conserved amino acid residue) and such substituted amino acid residue may or may not be one encoded by the genetic code; or it may be one in which one or more of the amino acid residues includes a substituent group; or it may be one in which the polypeptide is fused with another compound, such as a compound to increase the half life of the polypeptide (for example, polyethylene glycol); or it may be one in which the additional amino acids are fused to the polypeptide, such as a leader or secretory sequence or a sequence that is employed for purification of the polypeptide or a proprotein sequence. Such fragments, derivatives and analogs are within the scope of the present invention. In various aspects, the polypeptides of the invention may be partially purified, or purified product. 10170] A polypeptide of the present invention can have an amino acid sequence that is identical to that of the antibodies described herein, e.g., summarized in Tables 2 or 3, or that is different by minor variations due to one or more amino acid substitutions. The variation may be a "conservative change" typically in the range of about 1 to 5 amino acids, wherein the substituted amino acid has similar structural or chemical properties, e.g., replacement of leucine with isoleucine or threonine with serine; replacement of lysine with arginine or histidine. In contrast, variations may include nonconservative changes, e.g., replacement of a glycine with a tryptophan. Similar minor variations may also include amino acid deletions or insertions or both. Guidance in determining which and how many amino acid residues may be substituted, inserted, or deleted without changing biological or immunological activity may be found using computer programs known in the art, for example DNASTAR software (DNASTAR, Inc., Madison, Wis.). 56 WO 2011/050242 PCTIUS2010/053686 [0171] In another aspect, the invention features, isolated and/or recombinant nucleic acids encoding anti-GCC antibody molecules. In embodiments, the nucleic acids encode one or more of an antibody molecule, a heavy chain, a light chain, a light chain variable region, a heavy chain variable region, portions of the heavy chains and light chains of the antibody molecules described herein (e.g., a light chain variable region fragment which when paired with a full length heavy chain variable region is antigen binding, or a heavy chain variable region fragment which when paired with a full length light chain variable region is antigen binding), and CDRs. Embodiments include such nucleic acids disposed in vectors, e.g., expression vectors. In specific embodiments the invention includes plasmids pTOK58D-5F9LC and pTOK58D-5F9HC. Still further, the invention encompasses antibody molecules produced by host cells, e.g., expressing the antibody molecules encoded by plasmids pTOK58D-5F9LC and pTOK58D-5F9HC. [01721 In an embodiment, is provided a vector, e.g., an expression vector, comprising one or both of: [01731 sequences encoding a light chain variable region, e.g., sequences listed in Table 4, an antigen binding fragment thereof, or one, two or three CDRs from a light chain (and optionally a framework region), described herein, e.g., in Table 6; and [01741 sequences encoding a heavy chain variable region, e.g., sequences listed in Table 4, an antigen binding fragment thereof, or one, two or three CDRs from a heavy chain (and optionally a framework region), described herein, e.g., in Table 6. 101751 in embodiments provided, polynucleotides encode at least one heavy chain variable region or at least one light chain variable region of the antibodies of the present invention. In embodiments provided, polypeptides can encode at least one heavy chain variable region and one light chain variable region of the antibodies of the present invention. [0176] In an embodiment the anti-GCC antibody molecule comprises one or both of: (a) a light chain variable region, or an antigen binding fragment thereof, encoded by a nucleic acid that hybridizes under selected stringency conditions with, (i) the complement of an anti-GCC antibody molecule-encoding-nucleic acid sequence described herein, e.g., in Table 4, or (ii) any nucleic acid sequence that encodes a light chain of an 57 WO 2011/050242 PCT/US2010/053686 anti-GCC antibody molecule of the invention, e.g., one of the above-referenced human hybridoma, selected lymphocyte, or urine antibodies summarized in Tables 1 and 2; and (b) a heavy chain variable region, or an antigen binding fragment therof, encoded by a nucleic acid that hybridizes under selected stringency conditions with, (i) the complement of an anti-GCC antibody molecule-encoding-nucleic acid sequence described herein, e.g., in Table 4, or (ii) any nucleic acid sequence that encodes a heavy chain of an anti-GCC antibody molecule of the invention, e.g., one of the above-referenced human hybridoma, selected lymphocyte, or murine antibodies summarized in Tables I and 2. [0177] In an embodiment selected stringency conditions are high stringency or very high stringency conditions, e.g., as those conditions are described herein. [0178] In additional aspects, the antibody or the antigen binding fragment comprises an amino acid sequence of the light chain variable region amino acid sequence of the antibody encoded by the DNA having ATCC Accession Number PTA-8132. In other additional aspects, the antibody or the antigen binding fragment comprises an amino acid sequence of the heavy chain variable region sequence of the antibody encoded by the DNA having ATCC Accession Number PTA-8132. [0179] The present invention also provides vectors that include the polynucleotides of the present invention, host cells which are genetically engineered with vectors of the present invention and the production of the antibodies of the present invention by recombinant techniques. [0180] The appropriate DNA sequence may be inserted into the vector by a variety of procedures. In general, the DNA sequence is inserted into appropriate restriction endonuclease sites by procedures known in the art. The polynucleotide sequence in the expression vector is operatively linked to an appropriate expression control sequence (i.e. promoter) to direct mRNA synthesis. Examples of such promoters include, but are not limited to, the Rous sarcoma virus LTR or the early or late SV40 promoter, the E. coli lac or trp, the phage lambda PL promoter and other promoters known to control expression of genes in prokaryotic (e.g., tac, T3, T7 promoters for E coli) or eukaryotic (e.g., cytomegalovirus promoter, adenovirus late promoter, EF-1 a promoter) cells or their viruses. The expression vector also contains a ribosome binding site for translation 58 WO 2011/050242 PCT/US2010/053686 initiation and a transcription terminator. The vector may also include appropriate sequences for amplifying expression. For example, the vector can contain enhancers, which are transcription-stimulating DNA sequences of viral origin, such as those derived form simian virus such as SV40, polyoma virus, cytomegalovirus, bovine papilloma virus or Moloney sarcoma virus, or genomic, origin. The vector preferably also contains an origin of replication. The vector can be constructed to contain an exogenous origin of replication or, such an origin of replication can be derived from SV40 or another viral source, or by the host cell chromosomal replication mechanism. [0181] In addition, the vectors optionally contain a marker gene for selection of transfected host cells such as dihydrofolate reductase marker genes to permit selection with methotrexate in a variety of hosts, or antibiotics, such as p-lactamase gene (ampicillin resistance), Tet gene (for tetracycline resistance) used in prokaryotic cells or neomycin, GA418 (geneticin, a neomycin-derivative) gpt (mycophenolic acid), ampicillin, or hygromycin resistance genes, or genes which complement a genetic lesion of the host cells such as the absence of thymidine kinase, hypoxanthine phosphoribosyl transferase, dihydrofolate reductase, etc. Genes encoding the gene product of auxotrophic markers of the host (e.g., LEU2, URA3, HIS3) are often used as selectable markers in yeast. [01821 In order to obtain the antibodies of the present invention, one or more polynucleotide sequences that encode fbr the light and heavy chain variable regions and light and heavy chain constant regions of the antibodies of the present invention should be incorporated into a vector. Polynucleotide sequences encoding the light and heavy chains of the antibodies of the present invention can be incorporated into one or multiple vectors and then incorporated into the host cells. [0183] Suitable expression vectors for expression in mammalian cells include, for example, pCDM8, pCDNA1.1/amp, pcDNA3.1, pRc/RSV, pEF-1 (Invitrogen Life Technologies, Carlsbad, CA), pCMV-SCRIPT, pFB, pSG5, pXTI (Stratagene, La Jolla, CA), pCDEF3 (Goldman, L.A., et al., Biotechniques, 21:1013-1015 (1996)), pSVSPORT (GIBCO division of Invitrogen Life Technologies, Carlsbad, CA), pEF-Bos (Mizushima, S., et aL, Nucleic Acids Res., 18:5322 (1990)), Bicistronic GPEX® Retrovector (Gala Biotech, Middleton, WI) and the like. Expression vectors which are suitable for use in various expression hosts, such as prokaryotic cells (. coli), insect cells (Drosophila Schnieder S2 cells, Sf9) and yeast (P. methanolica, P. pastoris, S. cerevisiae) are also 59 WO 2011/050242 PCT/US2010/053686 available. Exemplary vectors are pLKTOK58 (wild type IgG1 Fc sequence) and pLKTOK59 (mutated IgGI Fc sequence) (see U.S. Patent Application publication no. 20060147445). [0184] As will be appreciated, antibodies in accordance with the present invention can be expressed in cell lines other than hybridoma cell lines. Sequences encoding the cDNAs or genomic clones for the particular antibodies can be used for a suitable mammalian or nonmammalian host cells. Transformation can be by any known method for introducing polynucleotides into a host cell, including, for example packaging the polynucleotide in a virus (or into a viral vector) and transducing a host cell with the virus (or vector) or by transfection procedures known in the art, for introducing heterologous polynucleotides into mammalian cells, e.g., dextran-mediated transfection, calcium phosphate precipitation, polybrene mediated transfection, protoplast fusion, electroporation, encapsulation of the polynucleotide(s) into liposomes and direct micioinjection of the DNA molecule. The transformation procedure used depends upon the host to be transformed. Methods for introduction of heterologous polynucleotides into mammalian cells are known in the art and include, but are not limited to, dextran-mediated transfection, calcium phosphate precipitation, polybrene mediated transfection, protoplast fusion, electroporation, particle bombardment, encapsulation of the polynucleotide(s) in liposomes, peptide conjugates, dendrimers, and direct microinjection of the DNA into nuclei. [0185] In another aspect, the invention features, a host cell comprising a nucleic acid described herein. In embodiments the cell expresses an antibody molecule, or component thereof, described herein. Still further embodiment provides a method of producing an antibody molecule, e.g., an anti-GCC antibody molecule described herein, e.g. a human or humanized antibody molecule comprising maintaining the host cell under conditions appropriate for expression, whereby immunoglobulin chain(s) are expressed and an antibody molecule is produced. An additional embodiment provides a host cell comprising any of the foregoing expression vectors encoding heavy and light chain antibody sequences. The host cell can be a eukaryotic cell, e.g., a mammalian cell, an insect cell, a yeast cell, or a prokaryotic cell, e.g., E. coli. For example, the mammalian cell can be a cultured cell or a cell line. Exemplary mammalian cells include lymphocytic cell lines (e.g., NSO), Chinese hamster ovary cells (CHO), COS cells. In a particular embodiment, the cultured host cell is a COS cell comprising nucleic acid sequences 60 WO 2011/050242 PCT/LJS2010/053686 encoding a 5F9 antibody molecule. In another embodiment, the host cell is Hybridoma 5F9 (PTA-8132). Additionally cells include oocyte cells, and cells from a transgenic animal, e.g., mammary epithelial cell. For example, nucleic acids encoding an antibody molecule described herein can be expressed in a transgenic nonhuman animal. [0186] Mammalian cell lines available as hosts for expression are known in the art and include many immortalized cell lines available from the American Type Culture Collection (ATCC), including but not limited to Chinese hamster ovary (CHO) cells, NSO cells, HeLa cells, baby hamster kidney (BHK) cells, monkey kidney cells (COS), human hepatocellular carcinoma cells (e.g., Hep G2), and a number of other cell lines. Non mammalian cells including but not limited to bacterial, yeast, insect, and plants can also be used to express recombinant antibodies. Site directed mutagenesis of the antibody CH2 domain to eliminate glycosylation may be preferred in order to prevent changes in either the immunogenicity, pharmacokinetic, and/or effector functions resulting from non-human glycosylation. The expression methods are selected by determining which system generates the highest expression levels and produce antibodies with constitutive GCC binding properties. [01871 A still further embodiment provides a method of producing an anti-GCC antibody molecule, e.g., a human or humanized antibody molecule, comprising maintaining the host cell comprising nucleic acids described herein, e.g., one or more nucleic acid sequence listed in Table 4 or 6, under conditions appropriate for expression of an immunoglobulin, whereby immunoglobulin chains, are expressed and an antibody molecule, e.g., a human or humanized antibody molecule that binds GCC, or a fragment or variant thereof, is produced. For example, methods of expression of antibody molecules include the use of host cells wherein a first recombinant nucleic acid molecule encoding an antibody molecule, e.g., a human or humanized antibody light chain, and a second recombinant nucleic acid molecule encoding an antibody molecule, e.g., a human or humanized antibody heavy chain, are comprised in a single expression vector. In other embodiments, they are in separate vectors. The method can further comprise the step of isolating or recovering the antibody, antigen-binding fragment of an antibody, antibody chain or antigen-binding fragment of an antibody chain, if desired. [01881 For example, a nucleic acid molecule (i.e., one or more nucleic acid molecules) encoding the heavy and light chains of a human antibody that binds a GCC 61 WO 20111050242 PCT/US2010/053686 protein, or an expression construct (i.e., one or more constructs) comprising such nucleic acid molecule(s), can be introduced into a suitable host cell to create a recombinant host cell using any method appropriate to the host cell selected (e.g., transformation, transfection, electroporation, infection), such that the nucleic acid molecule(s) are operably linked to one or more expression control elements (e.g., in a vector, in a construct created by processes in the cell, integrated into the host cell genome). The resulting recombinant host cell can be maintained under conditions suitable for expression (e.g., in the presence of an inducer, in a suitable non-human animal, in suitable culture media supplemented with appropriate salts, growth factors, antibiotics, nutritional supplements, etc.), whereby the encoded polypeptide(s) are produced. If desired, the encoded protein can be isolated or recovered (e.g., from the animal, the host cell, medium, milk). This process encompasses expression in a host cell of a transgenic non-human animal (see, e.g., WO 92/03918, GenPharm International) or plant. [0189] Further, expression of antibodies of the invention (or other moieties therefrom) from production cell lines can be enhanced using a number of known techniques. For example, the glutamine synthetase and DHFR gene expression systems are common approaches for enhancing expression under certain conditions. High expressing cell clones can be identified using conventional techniques, such as limited dilution cloning, Microdrop technology, or any other methods known in the art. The GS system is discussed in whole or part in connection with European Patent Nos. 0 216 846, 0 256 055, and 0 323 997 and European Patent Application No. 89303964.4. [0190] In an exemplary system for recombinant expression of a modified antibody, or antigen-binding portion thereof, of the invention, a recombinant expression vector encoding both the antibody heavy chain and the antibody light chain is introduced into dhfr- CHO cells by calcium phosphate-mediated transfection. Within the recombinant expression vector, the antibody heavy and light chain genes are each operatively linked to enhancer/promoter regulatory elements (e.g., derived from SV40, CMV, adenovirus and the like, such as a CMV enhancer/AdMLP promoter regulatory element or an SV40 enhancer/AdMLP promoter regulatory element) to drive high levels of transcription of the genes. The recombinant expression vector also carries a DHFR gene, which allows for selection of CHO cells that have been transfected with the vector using methotrexate selection/amplification. The selected transformant host cells are cultured to allow for expression of the antibody heavy and light chains and intact antibody is recovered from the 62 WO 2011/050242 PCT/US2010/053686 culture medium. Standard molecular biology techniques are used to prepare the recombinant expression vector, transfect the host cells, select for transformants, culture the host cells and recover the antibody from the culture medium. [01911 Antibodies of the invention can also be produced transgenically through the generation of a mammal or plant that is transgenic for the immunoglobulin heavy and light chain sequences of interest and production of the antibody in a recoverable form therefrom. In connection with the transgenic production in mammals, antibodies can be produced in, and recovered from, the milk of goats, cows, or other mammals. See, e.g., U.S. Pat. Nos. 5,827,690, 5,756,687, 5,750,172, and 5,741,957. [0192] The antibodies, antigen-binding fragments, antibody chains and antigen binding portions thereof described herein also can be produced in a suitable in vitro expression system, by chemical synthesis or by any other suitable method. Fusion Proteins and Immunoconjugates [01931 The anti-GCC antibodies described herein can be functionally linked by any suitable method (e.g., chemical coupling, genetic fusion, noncovalent association or otherwise) to one or more non-antibody molecular entities. [0194] Fusion proteins can be produced in which an anti-GCC antibody molecule as described herein and a non-antibody moiety are components of a single continuous polypeptide chain. The non-antibody moiety can be located N-terminally, C-terminally, or internally, with respect to the antibody moiety. For example, some embodiments can be produced by the insertion of a nucleic acid encoding immunoglobulin sequences into a suitable expression vector, such as a pET vector (e.g., pET-15b, Novagen), a phage vector (e.g., pCNATAB 5 E, Pharmacia), or other vector, e.g., pRIT2T Protein A fusion vector, Pharmacia). The resulting construct can be expressed to produce antibody chains that comprise a non-antibody moiety (e.g., Histidine tag, E tag, or Protein A IgG binding domain). Fusion proteins can be isolated or recovered using any suitable technique, such as chromatography using a suitable affinity matrix (see, e.g., Current Protocols in Molecular Biology (Ausubel, F.M et al., eds., Vol. 2, Suppl. 26, pp. 16.4.1-16.7.8 (1991)). [0195] The invention provides anti-GCC antibody molecules which are directed to and, in embodiments, are internalized into cells. They are capable of delivering therapeutic agents or detectable agents to or into cells expressing GCC, but not to or into 63 WO 2011/050242 PCT/US2010/053686 cells where the target is not expressed. Thus, the invention also provides anti-GCC immunoconjugates comprising an anti-GCC antibody molecule as described herein, which is conjugated to a therapeutic agent or a detectable agent. In embodiments, the affinity for GCC of an anti-GCC immunoconjugate is at least 10, 25, 50, 75, 80, 90, or 95% of that for the unconjugated antibody. This can be determined using cell surface GCC or isolated GCC. In an embodiment the anti-GCC antibody molecule, e.g., an immunoconjugate, has an LD50, as determined by an assay described herein, of less than 1,000, 500, 250, 100, or 50 pM. [01961 The anti-GCC antibody molecule can be modified to act as an immunoconjugate utilizing techniques that are known in the art. See e.g., Vitetta Immunol Today 14:252 (1993). See also U.S. Pat. No. 5,194,594. The preparation of radiolabeled antibodies can also be readily prepared utilizing techniques that are known in the art. See e.g., Junghans et al. in Cancer Chemotherapy and Biotherapy 655-686 (2d edition, Chafner and Longo, eds., Lippincott Raven (1996)). See also U.S. Pat. Nos. 4,681,581, 4,735,210, 5,101,827, 5,102,990 (U.S. Re. Pat. No. 35,500), 5,648,471, and 5,697,902. [0197] In some embodiments, the antibody molecule and non-antibody moiety are connected by means of a linker. In such embodiments, the immunoconjugate is represented by formula (I): AbiX-Z) wherein, Ab is an anti-GCC antibody molecule described herein; X is a moiety which connects Ab and Z, e.g., the residue of a linker described herein after covalent linkage to one or both of Ab and Z; Z is a therapeutic agent or label; and m ranges from about I to about 15. 64 WO 2011/050242 PCT/US2010/053686 [0198] The variable m represents the number of -X-Z moieties per antibody molecule in an immunoconjugate of formula (1). In various embodiments, m ranges from 1to 15, 1 to 10, 1to9, 1 to8, 1to7, I to6, 1to5,1 to4, I to3,orlto2. Insome embodiments, m ranges from 2 to 10, 2 to 9, 2 to 8, 2 to 7, 2 to 6, 2 to 5, 2 to 4 or 2 to 3. In other embodiments, m is 1, 2, 3, 4, 5 or 6. In compositions comprising a plurality of immunoconjugates of formula (1), m is the average number of-X-Z moieties per Ab, also referred to as the average drug loading. Average drug loading may range from 1 to about 15 -X-Z moieties per Ab. In some embodiments, when m represents the average drug loading, m is about 1, about 2, about 3, about 4, about 5, about 6, about 7, or about 8. In exemplary embodiments, m is from about 2 to about 8. In one embodiment, m is about 8. In another embodiment, m is about 4. In another embodiment, i is about 2. [0199] The average number of-X-Z moieties per Ab may be characterized by conventional means such as mass spectroscopy, ELISA assay, and HPLC. The quantitative distribution of immunoconjugates in terms of m may also be determined. In some instances, separation, purification, and characterization of homogeneous immunoconjugates where n is a certain value, as distinguished from immunoconjugates with other drug loadings, may be achieved by means such as reverse phase HPLC or electrophoresis. [0200] The immunoconjugates of formula (1) may exist as mixtures, wherein each component of the mixture has a different m value. For example, an immunoconjugate of formula (1) may exist as a mixture of two separate immunoconjugate components: one immunoconjugate component wherein m is 7, and the other immunoconjugate component wherein m is 8. [0201] In one embodiment, the immunoconjugate of formula (1) exists as a mixture of three separate immunoconjugates wherein m for the three separate immunoconjugates is 1, 2, and 3, respectively. [0202] In one embodiment, the immunoconjugate of formula (1) exists as a mixture of three separate immunoconjugates wherein m for the three separate immunoconjugates is 3, 4, and 5, respectively. [02031 In one embodiment, the immunoconjugate of formula (1) exists as a mixture of three separate immunoconjugates wherein m for the three separate immunoconjugates is 5, 6, and 7, respectively. 65 WO 2011/050242 PCT/US2010/053686 [0204] In one embodiment, the immunoconjugate of formula (1) exists as a mixture of three separate immunoconjugates wherein m for the three separate immunoconjugates is 7, 8, and 9, respectively. [02051 In one embodiment, the immunoconjugate of formula (1) exists as a mixture of three separate immunoconjugates wherein m for the three separate immunoconjugates is 9, 10, and 11, respectively. [0206] In one embodiment, the immunoconjugate of formula (Q) exists as a mixture of three separate immunoconjugates wherein in for the three separate immunoconjugates is 11, 12, and 13, respectively. [0207] In one embodiment, the immunoconjugate of formula (1) exists as a mixture of three separate immunoconjugates wherein m for the three separate immunoconjugates is 13, 14, and 15, respectively. [02081 A variety of suitable linkers (e.g., heterobifunctional reagents for connecting an antibody molecule to a therapeutic agent or label) and methods for preparing immunoconjugates are known in the art. (See, for example, Chari et at., Cancer Research 52:127-131 (1992).) The linker can be cleavable, e.g., under physiological conditions., e.g., under intracellular conditions, such that cleavage of the linker releases the drug (therapeutic agent or label) in the intracellular environment. In other embodiments, the linker is not cleavable, and the drug is released, for example, by antibody degradation. [0209] The linker can be bonded to a chemically reactive group on the antibody moiety, e.g., to a free amino, imino, hydroxyl, thiol or carboxyl group (e.g., to the N- or C terminus, to the epsilon amino group of one or more lysine residues, the free carboxylic acid group of one or more glutamic acid or aspartic acid residues, or to the sulfhydryl group of one or more cysteinyl residues). The site to which the linker is bound can be a natural residue in the amino acid sequence of the antibody moiety or it can be introduced into the antibody moiety, e.g., by DNA recombinant technology (e.g., by introducing a cysteine or protease cleavage site in the amino acid sequence) or by protein biochemistry (e.g., reduction, pH adjustment or proteolysis). [0210] One of the most commonly used non-specific methods of covalent attachment is the carbodiimide reaction to link a carboxy (or amino) group of a compound to amino (or carboxy) groups of the antibody molecule. Additionally, bifunctional agents 66 WO 2011/050242 PCTUS2010/053686 such as dialdehydes or imidoesters have been used to link the amino group of a compound to amino groups of an antibody molecule. Also available for attachment of drugs to antibody molecules is the Schiff base reaction. This method involves the periodate oxidation of a drug that contains glycol or hydroxy groups, thus forming an aldehyde which is then reacted with the antibody molecule. Attachment occurs via formation of a Schiff base with amino groups of the antibody molecule. Isothiocyanates can also be used as coupling agents for covalently attaching drugs to antibody molecule. Other techniques are known to the skilled artisan and within the scope of the present invention. [0211] In certain embodiments, an intermediate, which is the precursor of the linker (X), is reacted with the drug (Z) under appropriate conditions. In certain embodiments, reactive groups are used on the drug and/or the intermediate. The product of the reaction between the drug and the intermediate, or the derivatized drug, is subsequently reacted with the antibody molecule under appropriate conditions. [0212] The immunoconjugate can be purified from reactants by employing methodologies well known to those of skill in the art, e.g., column chromatography (e.g., affinity chromatography, ion exchange chromatography, gel filtration, hydrophobic interaction chromatography), dialysis, diafiltration or precipitation. The immunoconjugate can be evaluated by employing methodologies well known to those skilled in the art, e.g., SDS-PAGE, mass spectroscopy, or capillary electrophoresis. [0213] In some embodiments, the linker is cleavable by a cleaving agent that is present in the intracellular environment (e.g., within a lysosome or endosome or caveolea). The linker can be, e.g., a peptidyl linker that is cleaved by an intracellular peptidase or protease enzyme, including, but not limited to, a lysosomal or endosomal protease. In some embodiments, the peptidyl linker is at least two amino acids long or at least three amino acids long. Cleaving agents can include cathepsins B and D and plasmin, all of which are known to hydrolyze dipeptide drug derivatives resulting in the release of active drug inside target cells (see, e.g., Dubowchik and Walker, 1999, Pharn. Therapeutics 83:67-123). Most typical are peptidyl linkers that are cleavable by enzymes that are present in GCC-expressing cells. For example, a peptidyl linker that is cleavable by the thiol-dependent protease cathepsin-B, which is highly expressed in cancerous tissue, can be used (e.g., a Phe-Leu or a Gly-Phe-Leu-Gly linker (SEQ ID NO:319)). Other examples of such linkers are described, e.g., in U.S. Patent No. 6,214,345, incorporated herein by 67 WO 2011/050242 PCT/US2010/053686 reference in its entirety and for all purposes. In a specific embodiment, the peptidyl linker cleavable by an intracellular protease is a Val-Cit linker or a Phe-Lys linker (see, e.g., U.S. patent 6,214,345, which describes the synthesis of doxorubicin with the val-cit linker). One advantage of using intracellular proteolytic release of the therapeutic agent is that the agent is typically attenuated when conjugated and the serum stabilities of the conjugates are typically high. [0214] In other embodiments, the cleavable linker is pH-sensitive, i.e., sensitive to hydrolysis at certain pH values. Typically, the pH-sensitive linker is hydrolyzable under acidic conditions. For example, an acid-labile linker that is hydrolyzable in the lysosome (e.g., a hydrazone, semicarbazone, thiosemicarbazone, cis-aconitic amide, orthoester, acetal, ketal, or the like) can be used. (See, e.g., U.S. Patent Nos. 5,122,368; 5,824,805; 5,622,929; Dubowchik and Walker, 1999, Pharm. Therapeutics 83:67-123; Neville et al., 1989, Biol. Chem. 264:14653-14661.) Such linkers are relatively stable under neutral pH conditions, such as those in the blood, but are unstable at below pH 5.5 or 5.0, the approximate pH of the lysosome. In certain embodiments, the hydrolyzable linker is a thioether linker (such as, e.g., a thioether attached to the therapeutic agent via an acyihydrazone bond (see, e.g., U.S. Patent No. 5,622,929). [0215] In yet other embodiments, the linker is cleavable under reducing conditions (e.g., a disulfide tinker). A variety of disulfide linkers are known in the art, including, for example, those that can be formed using SATA (N-succinimidyl-S-acetylthioacetate), SPDP (N-succinimidyl-3-(2-pyridydithio)propionate), SPDB (N-succinimidyl-3-(2 pyridyldithio)butyrate) and SMPT (N-succinimidyl-oxycarbonyl-alpha-methyl-alpha-(2 pyridyl-dithio)toluene), SPDB and SMPT (See, e.g., Thorpe et al., 1987, Cancer Res. 47:5924-5931; Wawrzynczak et aL, In Immunoconjugates: Antibody Conjugates in Radioimagery and Therapy of Cancer (C. W. Vogel ed., Oxford U. Press, 1987. See also U.S. Patent No. 4,880,935.) [0216] In yet other specific embodiments, the linker is a malonate linker (Johnson et aL, 1995, Anticancer Res. 15:1387-93), a maleimidobenzoyl linker (Lau et al., 1995, Bioorg Med Chem. 3(10):1299-1304), or a 3'-N-amide analog (Lau et al., 1995, Bioorg Med-Chem. 3(10):1305-12). 68 WO 2011/050242 PCT/LTS2010/053686 [0217] In yet other embodiments, the linker unit is not cleavable and the drug is released by antibody degradation. (See for example U.S. Publication No. 20050238649 incorporated by reference herein in its entirety and for all purposes). [0218] Typically, the linker is not substantially sensitive to the extracellular environment. As used herein, "not substantially sensitive to the extracellular environment," in the context of a linker, means that no more than about 20%, typically no more than about 15%, more typically no more than about 10%, and even more typically no more than about 5%, no more than about 3%, or no more than about 1% of the linkers, in a sample of immunoconjugate, are cleaved when the immunoconjugate presents in an extracellular environment (e.g., in plasma). Whether a linker is not substantially sensitive to the extracellular environment can be determined, for example, by incubating with plasma the immunoconjugate for a predetermined time period (e.g., 2, 4, 8, 16, or 24 hours) and then quantitating the amount of free drug present in the plasma. [0219] In other, non-mutually exclusive embodiments, the linker promotes cellular internalization. In certain embodiments, the linker promotes cellular internalization when conjugated to the therapeutic agent or label (Z). In yet other embodiments, the linker promotes cellular internalization when conjugated to both the Z moiety and the anti-GCC antibody molecule. [0220] A variety of exemplary linkers that can be used with the present compositions and methods are described in WO 2004-010957, U.S. Publication No. 20060074008, U.S. Publication No. 20050238649, and U.S. Publication No. 20060024317 (each of which is incorporated by reference herein in its entirety and for all purposes). [02211 Examples of linkers capable of being used to couple an antibody molecule to a therapeutic agent or label include, for example, maleimidocaproyl (mc); maleimidocaproyl-p-aminobenzylcarbamate; maleimidocaproyl-peptide aminobenzylcarbamate linkers, e.g., maleimidocaproyl-L-phenylalanine-L-lysine-p aminobenzylcarbamate and maleimidocaproyl-L-valine-L-citrulline-p aminobenzylcarbamate (vc); N-succinimidyl 3-(2-pyridyldithio)proprionate (also known as N-succinimidyl 4-(2-pyridyldithio)pentanoate or SPP); 4-succinimidyl-oxycarbonyl-2 methyl-2-(2-pyridyldithio)-toluene (SMPT); N-succinimidyl 3-(2-pyridyldithio)propionate (SPDP); N-succinimidyl 4-(2-pyridyldithio)butyrate (SPDB); 2-iminothiolane; S acetylsuccinic anhydride; disulfide benzyl carbamate; carbonate; hydrazone linkers; N-(a 69 WO 2011/050242 PCT/US2010/053686 Maleimidoacetoxy) succinimide ester; N-[4-(p-Azidosalicylamido) butyl]-3'-(2' pyridyldithio)propionamide (AMAS); N-[ p-Maleimidopropyloxy]succinimide ester (BMPS); [N-e-Maleimidocaproyloxy]succinimide ester (EMCS); N-[y Maleimidobutyryloxy]succinimide ester (GMBS); Succinimidyl-4-[N Maleimidomethyl]cyclohexane-1-carboxy-[6-amidocaproate] (LC-SMCC); Succinimidyl 6-(3-[2-pyridyldithio]-propionamido)hexanoate (LC-SPDP); m-Maleimidobenzoyl-N hydroxysuccinimide ester (MBS); N-Succinimidyl[4-iodoacetyl]aminobenzoate (SIAB); Succinimidyl 4-[N-maleimidomethyl]cyclohexane-1-carboxylate (SMCC); N-Succinimidyl 3-[2-pyridyldithio]-propionamido (SPDP); [N-e-Maleinidocaproyloxy]suffosuccinimide ester (Sulfo-EMCS); N-[y-Maleimidobutyryloxy]sulfosuccinimide ester (Sulfo-GMBS); 4-Sulfosuccinimidyl-6-methyl-c-(2-pyridyldithio)toluamido]hexanoate) (Sulfo-LC SMPT); Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate (Sulfo-LC SPDP); m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester (Sulfo-MBS); N Sulfosuccinimidyl[4-iodoacetyl]aminobenzoate (Sulfo-SIAB); Sulfosuccinimidyl 4-[N maleimidomethyl]cyclohexane- I -carboxylate (Sulfo-SMCC); Sulfosuccinimidyl 4 -[p maleimidophenyl]butyrate (Sulfo-SMPB); ethylene glycol-bis(succinic acid N hydroxysuccinimide ester) (EGS); disuccinimidyl tartrate (DST); 1,4,7,10 tetraazacyclododecane- 1,4,7,1 0-tetraacetic acid (DOTA); diethylenetriamine-pentaacetic acid (DTPA); and thiourea linkers. [0222] In some embodiments, the linker -X- has the formula -Aa-Ww-Yy-, and the immunoconjugate of formula () is characterized by formula (I): Ab Aa-W-Y'-Z) wherein, Ab is an anti-GCC antibody molecule described herein; -A- is a Stretcher unit; a is 0 or 1; each -W- independently is an Amino Acid unit; w is an integer ranging from 0 to 12; 70 WO 2011/050242 PCT/US2010/05368 6 -Y- is a self-immolative spacer unit; y is 0, 1, or 2; Z is a therapeutic agent or label; and m ranges from about I to about 15. [0223] The Stretcher unit ( A ), when present, is capable of linking an Ab unit to an Amino Acid unit (-W-), if present, to a Spacer unit (-Y-), if present; or to a therapeutic agent or label ( Z ). Useful functional groups that can be present on an anti-GCC antibody molecule, either naturally or via chemical manipulation include, but are not limited to, sulfhydryl, amino, hydroxyl, the anomeric hydroxyl group of a carbohydrate, and carboxyl. Suitable functional groups are sulfhydryl and amino. In one example, sulfhydryl groups can be generated by reduction of the intramolecular disulfide bonds of an anti-GCC antibody molecule. In another embodiment, sulfhydryl groups can be generated by reaction of an amino group of a lysine moiety of an anti-GCC antibody molecule with 2-iminothiolane (Traut's reagent) or other sulfhydryl generating reagents. In certain embodiments, the anti-GCC antibody molecule is a recombinant antibody and is engineered to carry one or more lysines. In certain other embodiments, the recombinant anti-GCC antibody molecule is engineered to carry additional sulfhydryl groups, e.g., additional cysteines. [0224] In one embodiment, the Stretcher unit forms a bond with a sulfur atom of the Ab unit. The sulfur atom can be derived from a sulfhydryl group of an Ab. Representative Stretcher units of this embodiment are depicted within the square brackets of Formulas (Ia) and (M1b), wherein Ab-, -W-, -Y-, -Z, w and y are as defined above, and Ra is selected from -C-Cio alkylene-, -C-Cio alkenylene-, -C 2
-C
10 alkynylene-, carbocyclo-, -O-(C-Cs alkylene)-, 0-(C 2
-C
8 alkenylene)-, -O-(C 2 -Cs alcynylene)-, arylene-, -C 1 -Co alkylene-arylene-, -C 2 -CIO alkenylene-arylene, -C2-C1a alkynylene arylene, -arylene-C1-Cio alkylene-, -arylene-C 2 -Ci 0 alkenylene-, -arylene-C 2 -Ci 0 alkynylene-, -Cr-Clo alkylene-(carbocyclo)-, -C 2
-C
1 0 alkenylene-(carbocyclo)-, -C 2
-C
10 alkynylene-(carbocyclo)-, -(carbocyclo)-C-Cia alkylene-, -(carbocyclo)-C 2
-C
1 i alkenylene-, -(carbocyclo)-C 2 -C1o alkynylene, heterocyclo-, -C-Cio alkylene-( heterocyclo)-, -C 2
-C
10 alkenylene-(heterocyclo)-, -C 2 -C1o alcynylene-(heterocyclo)-, (heterocyclo)-C-Ci 0 alkylene-, -(heterocyclo)-C 2 -Cia alkenylene-, -(heterocyclo)-C 2 -Cio aLkynytene-, -(CH 2
CH
2 0)r, or -(CH 2
CH
2 0),CH 2 -, and r is an integer ranging from 1-10, 71 WO 2011/050242 PCT/US2010/053686 wherein said alkyl, alkenyl, alkynyl, alkylene, alkenylene, alkynylene, aryl, carbocyle, carbocyclo, heterocyclo, and arylene radicals, whether alone or as part of another group, are optionally substituted. In some embodiments, said alkyl, alkenyl, alkynyl, alkylene, alkenylene, alkynylene, aryl, carbocyle, carbocyclo, heterocyclo, and arylene radicals, whether alone or as part of another group, are unsubstituted. In some embodiments, Ra is selected from -C 1
-C
10 alkylene-, - carbocyclo-, -O-(CI-Cs alkylene)-, -arylene-, -Ci-Cio alkylene-aryene-, -arylene-C 1
-C
10 alkylene-, -C 1
-C
10 alkylene-(carbocyclo)-, -( carbocyclo)-Ci-Cio alkylene-, -C 3 -Cs heterocyclo-, -C 1
-C
10 alkylene-(heterocyclo)-, -( heterocyclo)-C 1 -Cio alkylene-, -(CH 2
CH
2 0)r, and -(CH 2
CH
2 0)rCH 2 -; and r is an integer ranging from 1-10, wherein said alkylene groups are unsubstituted and the remainder of the groups are optionally substituted. [02251 It is to be understood from all the exemplary embodiments that even where not denoted expressly, from I to 15 drug moieties can be linked to an Ab (m = 1-15). 0 Ab -i N-Ra-C W-Yy-Z [0226] (IIIa) O 0 Ab- -CH 2 -C-NH-R-C- W--Yy- Z [0227] (IIlb) [0228] An illustrative Stretcher unit is that of Formula (HIa) wherein Ra is (CH 2
)
5 -: O [0229] Another illustrative Stretcher unit is that of Formula (IIa) wherein R is
-(CH
2
CH
2 0)-CH 2 -; and r is 2: 72 WO 2011/050242 PCT/US2010/053686 0 0* [0230] Another illustrative Stretcher unit is that of Formula (lIa) wherein Ra is arylene- or arylene-C-C 10 alkylene-. In some embodiments, the aryl group is an unsubstituted phenyl group. [02311 Still another illustrative Stretcher unit is that of Formula (IIb) wherein Ra is -(CH 2 )s-: 0 /NH 0 [02321 In certain embodiments, the Stretcher unit is linked to the Ab unit via a disulfide bond between a sulfur atom of the Ab unit and a sulfur atom of the Stretcher unit. A representative Stretcher unit of this embodiment is depicted within the square brackets of Formula (IV), wherein Ra, Ab-, -W-, -Y-, -Z, w and y are as defined above. 0 [A2331 bjSfS-Ra-C mW-y-Z [0234] It should be noted that throughout this application, the S moiety in the formula below refers to a sulfur atom of the Ab unit, unless otherwise indicated by context. Ab-S-1 [0235] In yet other embodiments, the Stretcher, prior to attachment to Ab, contains a reactive site that can form a bond with a primary or secondary amino group of the Ab. Examples of these reactive sites include, but are not limited to, activated esters such as succinimide esters, 4 nitrophenyl esters, pentafluorophenyl esters, tetrafluorophenyl esters, anhydrides, acid chlorides, sulfonyl chlorides, isocyanates and isothiocyanates. Representative Stretcher units of this embodiment are depicted within the square brackets of Formulas (Va) and (Vb), wherein -Ra-, Ab-, -W-, -Y-, -Z, w and y are as defined above; 73 WO 2011/050242 PCTIUS2010/053686 0 0 Ab -C-NH Ra-C W-Yy-Z) [0236] m (Va) Ab -C-NHRa-C W,-Yy-Z [0237] (b) [0238] In some embodiments, the Stretcher contains a reactive site that is reactive to a modified carbohydrate's (-CHO) group that can be present on an Ab. For example, a carbohydrate can be mildly oxidized using a reagent such as sodium periodate and the resulting (-CHO) unit of the oxidized carbohydrate can be condensed with a Stretcher that contains a functionality such as a hydrazide, an oxime, a primary or secondary amine, a hydrazine, a thiosemicarbazone, a hydrazine carboxylate, and an aryihydrazide such as those described by Kaneko et aL, 1991, Bioconjugate Chem. 2:133-41. Representative Stretcher units of this embodiment are depicted within the square brackets of Formulas (VIa), (ib), and (VIc), wherein -R-, Ab-, -W-, -Y-, -Z, w and y are as defined as above. Ab4 N-NH R"-C W,-Yy )(V"a) 0 Ab EN-NH C-R-C -W -v-Yy-Z )m (VIC) [0239] The Amino Acid unit (-W-), when present, links the Stretcher unit to the Spacer unit if the Spacer unit is present, links the Stretcher unit to the Drug moiety if the 74 WO 2011/050242 PCT/US2010053686 Spacer unit is absent, and links the Ab unit to the therapeutic agent or label moiety if the Stretcher unit and Spacer unit are absent. W,- can be, for example, a monopeptide, dipeptide, tripeptide, tetrapeptide, pentapeptide, hexapeptide, heptapeptide, octapeptide, nonapeptide, decapeptide, undecapeptide or dodecapeptide unit. Each -W- unit independently has the formula denoted below in the square brackets, and w is an integer ranging from 0 to 12: H O
CH
3 0 iN YXi RN {Rb or Rb wherein R is hydrogen, methyl, isopropyl, isobutyl, sec-butyl, benzyl, p-hydroxybenzyl, CH 2 OH, -CH(OH)CH 3 , -CH 2
CH
2
SCH
3 , -CH 2 CONH2, -CH 2 COOH,
-CH
2
CH
2
CONH
2 , -CH 2
CH
2 COOH, -(CH 2
)
3
NHC(=NH)NH
2 , -(CH 2 )3NH 2 ,
-(CH
2
)
3
NHCOCH
3 , -(CH 2
)
3 NHCHO, -(CH 2
)
4
NHC(=NH)NH
2 , -(CH 2
)
4 NH2,
-(CH
2
)
4
NHCOCH
3 , -(CH 2
)
4 NHCHO, -(CH 2
)
3
NHCONH
2 , -(CH 2
)
4
NHCONH
2 ,
-CH
2
CH
2
CH(OH)CH
2
NH
2 , 2-pyridylmethyl-, 3 -pyridylmethyl-, 4-pyridylmethyl-, phenyl, cyclohexyl, OH
-CH
2 or CH2 75 H 75 WO 2011/050242 PCT/US2010/053686 [02401 In some embodiments, the Amino Acid unit can be enzymatically cleaved by one or more enzymes, including a cancer or tumor-associated protease, to liberate the therapeutic agent or label moiety (-Z), which in one embodiment is protonated in vivo upon release to provide a therapeutic agent or label (Z). [0241] In certain embodiments, the Amino Acid unit can comprise natural amino acids. In other embodiments, the Amino Acid unit can comprise non-natural amino acids. Illustrative W, units are represented by formulas (VH)-(IX): ORd [02421 R, O (V1) wherein R and Rd are as follows: R* Rd Benzyl (CH2)4NH 2 ; methyl (CH2) 4
NH
2 ; isopropyl (CH 2
)
4
NH
2 ; isopropyl (CH 2
)
3
NHCONH
2 ; benzyl (CH2)3NHCONH 2 ; isobutyl (CH 2
)
3
NHCONH
2 ; sec-butyl (CH 2
)
3
NHCONH
2 ; (CH 2
)
3 NHCONH2; N H benzyl methyl; benzyl (CH 2
)
3
NHC(=NH)NH
2 ; 0 Rd 0 R, O 0y r (Vii) wherein R", Rd and R* are as follows: R* Rd Re 76 WO 2011/050242 PCT/US2010/053686 benzyl Benzyl (CH 2
)
4 NH2; isopropyl Benzyl (CH 2
)
4
NH
2 ; and H Benzyl (CH 2
)
4
NH
2 ; H O Rd 0 R RC O R' O wherein R, Rd, R* and Rf are as follows: RC Rd R H benzyl isobutyl H; and methyl isobutyl methyl isobutyl. [02431 Exemplary Amino Acid units include, but are not limited to, units of formula (VII) where: R' is benzyl and Rd is -(CH 2
)
4
NH
2 ; R' is isopropyl and Rd is -(CH2) 4 N1 2 ; or R' is isopropyl. and Rd is -(CH 2
)
3
NHCONH
2 .Another exemplary Amino Acid unit is a unit of formula (VIII) wherein R is benzyl, Rd is benzyl, and R" is (CH 2
)
4
NH
2 . [02441 Useful -W,- units can be designed and optimized in their selectivity for enzymatic cleavage by a particular enzyme, for example, a tumor-associated protease. In one embodiment, a -W, - unit is that whose cleavage is catalyzed by cathepsin B, C arid D, or a plasmin protease. [02451 In one embodiment, -W,- is a dipeptide, tripeptide, tetrapeptide or pentapeptide. When Re, R4, Rd, R* or R is other than hydrogen, the carbon atom to which Rb, R*, Rd, R" or Rf is attached is chiral. [02461 Each carbon atom to which Rb, R", Rd, R" or R'is attached is independently in the (S) or (R) configuration. [02471 In one aspect of the Amino Acid unit, the Amino Acid unit is valine citrulline (vc or val-cit). In another aspect, the Amino Acid unit is phenylalanine-lysine (i.e., fk). In yet another aspect of the Amino Acid unit, the Amino Acid unit is N 77 WO 2011/050242 PCT/US2010/053686 methylvaline-citrulline. In yet another aspect, the Amino Acid unit is 5-aminovaleric acid, homo phenylalanine lysine, tetraisoquinolinecarboxylate lysine, cyclohexylalanine lysine, isonepecotic acid lysine, beta-alanine lysine, glycine seine valine glutamine and isonepecotic acid. [0248] The Spacer unit (-Y-), when present, links an Amino Acid unit to the therapeutic agent or label moiety (-Z-) when an Amino Acid unit is present. Alternatively, the Spacer unit links the Stretcher unit to the therapeutic agent or label moiety when the Amino Acid unit is absent. The Spacer unit also links the therapeutic agent or label moiety to the Ab unit when both the Amino Acid unit and Stretcher unit are absent. [0249] Spacer units are of two general types: non self-immolative or self immolative. A non self-immolative Spacer unit is one in which part or all of the Spacer unit remains bound to the therapeutic agent or label moiety after cleavage, particularly enzymatic, of an Amino Acid unit from the antibody- drug conjugate. Examples of a non self-immolative Spacer unit include, but are not limited to a (glycine-glycine) Spacer unit and a glycine Spacer unit (both depicted in Scheme 1) (infra). When a conjugate containing a glycine-glycine Spacer unit or a glycine Spacer unit undergoes enzymatic cleavage via an enzyme (e.g., a tumor-cell associated-protease, a cancer-cell-associated protease or a lymphocyte-associated protease), a glycine-glycine-Z moiety or a glycine-Z moiety is cleaved from Ab-Aa-Ww-. In one embodiment, an independent hydrolysis reaction takes place within the target cell, cleaving the glycine-Z moiety bond and liberating the therapeutic agent or label. 78 WO 2011/050242 PCT/US2010/053686 Scheme I Ab ±Aa-Ww-Gy-Z Ab A a -W-Gly-Gly-Z) enzymatic enzymatic cleavage cleavage Gly-Z Gly-Gly--Z hydrolysis hydrolysis therapeutic agent or label therapeutic agent or label [0250] In some embodiments, a non self-immolative Spacer unit (-Y-) is -Gly-. In some embodiments, a non self-inmmolative Spacer unit (-Y-) is -Gly-Gly-, [0251] In one embodiment, the invention provides an immunoconjugate of formula (I) wherein the Spacer unit is absent (y = 0), or a pharmaceutically acceptable salt thereof [0252] Alternatively, a conjugate containing a self-immolative Spacer unit can release -Z. As used herein, the term "self-immolative Spacer" refers to a bifunctional chemical moiety that is capable of covalently linking together two spaced chemical moieties into a stable tripartite molecule. It will spontaneously separate from the second chemical moiety if its bond to the first moiety is cleaved. [0253] In some embodiments, -Yy- is a p-aminobenzyl alcohol (PAB) unit (see Schemes 2 and 3) whose phenylene portion is substituted with Q, wherein Q is -C 1
-C
8 alkyl, -C 2 -C8 alkenyl, -C 2 -Cg alkynyl, -O-(CrCs alkyl), -O-(C 2 -Cs alkenyl), -O-(C 2 -Cs alkynyl), -halogen,-nitro or -cyano; and n is an integer ranging from 0-4. The alkyl, alkenyl and alkynyl groups, whether alone or as part of another group, can be optionally substituted. [0254] In some embodiments, -Y- is a PAB group that is linked to -Ww - via the amino nitrogen atom of the PAB group, and connected directly to -Z via a carbonate, carbamate or ether group. Without being bound by any particular theory or mechanism, 79 WO 2011/050242 PCT/US2010/053686 Scheme 2 depicts a possible mechanism of release of a therapeutic agent or label (-Z) from a PAB group that is attached directly to -Z via a carbamate or carbonate group as described by Toki et aL, 2002, J. Org. Chem. 67:1866-1872. Scheme 2 Qn Ab a-W,.--NH Z O )M enzymatic cleavage NH OC O 1,6-elimination Therapeutic agent or label [0255] In Scheme 2, Q is -C 1
-C
8 alkyl, -C 2
-C
8 alkenyl, -C 2
-C
8 alkynyl, -O-(C-Cs alkyl), -O-(C 2 -Cs alkenyl), -O-(C 2 -Cs alkynyl), -halogen, -nitro or -cyano; m is an integer ranging from 0-4; and m ranges from 1 to about 20. The alkyl, alkenyl and alkynyl groups, whether alone or as part of another group, can be optionally substituted. [0256] Without being bound by any particular theory or mechanism, Scheme 3 depicts a possible mechanism of release of a therapeutic agent or label moiety (-Z) from a PAB group which is attached directly to -Z via an ether or amine linkage, wherein -Z includes the oxygen or nitrogen group that is part of the therapeutic agent or label moiety. 80 WO 2011/050242 PCT/US2010/053686 Scheme 3 Qn A Aa-Ww-NHZ ~m enzymatic cleavage Qn N 2 I 1,6-elimination Qn7 NH + + Therapeutic agent or label [0257] In Scheme 3, Q is -C-Cs alkyl, -C 2 -Cs alkenyl, -C 2 -Cs alkynfl, -O-(CI-Cs alkyl), -O-(C 2 -Cs alkenyl), -O-(C 2
-C
8 alkynyl), -halogen, -nitro or -cyano; n is an integer ranging from 0-4; and m ranges from 1 to about 20. The alkyl, alkenyl and alkynyl groups, whether alone or as part of another group, can be optionally substituted. [0258] Other examples of self-immolative spacers include, but are not limited to, aromatic compounds that are electronically similar to the PAB group such as 2 aminoimidazol-5-methanol derivatives (Hay et al., 1999, Bioorg. Med. Chem. Lett. 9:2237) and ortho or para-aminobenzylacetals. Spacers can be used that undergo cyclization upon amide bond hydrolysis, such as substituted and unsubstituted 4 aminobutyric acid aides (Rodrigues et al., 1995, Chemistry Biology 2:223), appropriately substituted bicyclo[2.2.1] and bicyclo[2.2.2] ring systems (Storm et al., 1972, J. Amer. Chem. Soc. 94:5815) and 2-aminophenylpropionic acid amides (Amsberry et al., 1990, J. Org. Chem, 55:5867). Elimination of amine-containing drugs that are substituted at the a 81 WO 2011/050242 PCTfUS2010/053686 position of glycine (Kingsbury et al, 1984, J Med. Chem. 27:1447) are also examples of self-immolative spacers. [0259] In one embodiment, the Spacer unit is a branched bis(hydroxymethyl) styrene (BHMS) unit as depicted in Scheme 4, which can be used to incorporate and release multiple drugs. Scheme 4
H
2
(O(CO)))
0 -Z H CH2(O(CO))) Z) Ab Aa-Ww-NO Enzymatic cleavage (2 x m) therapeutic agents or labels [0260] In Scheme 4, Q is -C-C 8 alkyl, -( 2
-C
8 alkenyl, -C 2 -Cs alkynyl, -O-(C-Cs alkyl), -O-(C 2 -Cg alkenyl), -O-(C 2
-C
8 alkynyl), -halogen, -nitro or -cyano; n is an integer ranging from 0-4; o is 0 or 1; and m is an integer of from 1 to about 20. The alkyl, alkenyl and alkynyl groups, whether alone or as part of another group, can be optionally substituted. [0261] In some embodiments, the -Z moieties are the same. In yet another embodiment, the -Z moieties are different. [0262] In one aspect, Spacer units (-Yy-) are represented by Formulae (X)-(XII): 0 (A) wherein Q is -C-C 8 alkyl, -C 2
-C
8 alkenyl, -C 2
-C
8 alkynyl, -O-(C-C 8 alkyl), -O-(C 2 -Cs alkenyl), -O-(C 2
-C
8 alkynyl), -halogen, -nitro or -cyano; and m is an integer ranging from 82 WO 2011/050242 PCT/fUS2010/053686 0-4. The alkyl, alkenyl and alkynyl groups, whether alone or as part of another group, can be optionally substituted. )-H N-CH 2 -CO-j (XI) and j-NHCH 2
C()-NHCH
2 C(O)-j [0263] In a group of selected embodiments, the conjugates of Formula (1) and (II) are: Ab sW-Yy - :Z wherein w and y are each 0, 1 or 2: Ab St o Z wherein w and y are each 0; Ab Aa-HN ( Yy-Z O NH m O=
NH
2 83 WO 20111050242 PCT/JUS2010/053686 0 Ab Aa-HN O NH m
NH
2 00 Abff t JN-Yy- Z O H O )MH NH O=( NH2 ,and O Ab 'S O 0Z NH O=( NH2 wherein Aa, W., Yy, Z and Ab have the meanings provided above. [0264] The variable Z in formula (1) is a therapeutic agent or label. The therapeutic agent can be any agent capable of exerting a desired biological effect. In some embodiments, the therapeutic agent sensitizes the cell to a second therapeutic modality, e.g., a chemotherapeutic agent, radiation therapy, immunotherapy. [02651 In some embodiments, the therapeutic agent is a cytostatic or cytotoxic agent. Examples include, without limitation, antimetabolites (e.g., azathioprine, 6 mercaptopurine, 6-thioguanine, fludarabine, pentostatin, cladribine, 5-fluorouracil (5FU), 84 WO 2011/050242 PCT/US2010/053686 floxuridine (FUDR), cytosine arabinoside (cytarabine), methotrexate, trimethoprim, pyrimethamine, pemetrexed); alkylating agents (e.g., cyclophosphamide, mechlorethamine, uramustine, melphalan, chlorambucil, thiotepa/chlorambucil, ifosfamide, carmustine, lomustine, streptozocin, busulfan, dibromomannitol, cisplatin, carboplatin, nedaplatin, oxaliplatin, satraplatin, triplatin tetranitrate, procarbazine, altretamine, dacarbazine, mitozolomide, temozolomide); anthracyclines (e.g., daunorubicin, doxorubicin, epirubicin, idarubicin, valrubicin); antibiotics (e.g., dactinomycin, bleomycin, mithramycin, anthramycin, streptozotocin, gramicidin D, mitomycins (e.g., mitomycin C), duocarmycins (e.g., CC-1065), calicheamicins); antimitotic agents (including, e.g., maytansinoids, auristatins, dolastatins, cryptophycins, vinca alkaloids (e.g., vincristine, vinblastine, vindesine, vinorelbine), taxanes (e.g., paclitaxel, docetaxel, or a novel taxane (see, e.g., International Patent Publication No. WO 01/38318, published May 31, 200 1)), and colchicines; topoisomerase inhibitors (e.g., irinotecan, topotecan, amsacrine, etoposide, teniposide, mitoxantrone); and proteasome inhibitors (e.g., peptidyl boronic acids). Maytansinoid immunoconjugates [02661 In some embodiments, the therapeutic agent is a maytansinoid. Maytansinoid compounds and methods for their conjugation to antibodies are described, for example, in Chari et al., Cancer Res., 52: 127-131 (1992); Widdison et al., J. Med. Chem. 49: 4392-4408 (2006); and U.S. Patent Nos. 5,208,020 and 6,333,410. Examples of maytansinoids include maytansine analogues having a modified aromatic ring (e.g., C-19 dechloro, C-20-demethoxy, C-20-acyloxy) and those having modifications at other positions (e.g., C-9-CH, C-14-alkoxymethyl, C-14-hydroxymethyl or acyloxymethyl, C 15-hydroxy/acyloxy, C-15-methoxy, C-18-N-demethyl, 4,5-deoxy). In certain embodiments, the maytansinoid is N 2 -deacetyl-N 2 -(4-mercapto- 1 -oxopentyl)maytansine (DM3), N 2 '-deacetyl-N 2 '-(3-mercapto-1 -oxopropyl)-maytansine (DM1), or N 2 -deacetyl
N
2 '-(4-mercapto-4-methyl-1-oxopentyl)maytansine (DM4) [02671 Maytansinoid compounds that comprise a sulfhydryl group can be coupled to antibodies using a heterobifunctional linker that is connected to the maytansinoid compound by way of a thioether or disulfide linkage. In some such embodiments, the linker is coupled to an amino group on the antibody (e.g., a terminal amino group or the epsilon amino group of a lysine residue. In some embodiments, the heterobifunctional 85 WO 2011/050242 PCT/US2010/053686 linker that is used to couple a maytansinoid compounds to an antibody is N-succinimidyl 3-(2-pyridyldithio)proprionate (also known as N-succinimidyl 4-(2 pyridyldithio)pentanoate, or SPP), 4-succinimidyl-oxycarbonyl-2-methyl-2-(2 pyridyldithio)-toluene (SMPT), N-succinimidyl 4-[N-maleimidomethyl]cyclohexane-1 carboxylate (SMCC), N-succinimidyl 3-(2-pyridyldithio)propionate (SPDP); N succinimidyl 4-(2-pyridyldithio)butyrate (SPDB), 2-iminothiolane, or S-acetylsuccinic anhydride. [0268] In certain embodiments, the immunoconjugate of formula (1) is characterized by the formula Ab-(SMCC-DM1). (formula (I-1); Ab-(SPP-DM1). (formula (1-2); or Ab-(SPDB-DM4)m (formula (1-3), wherein Ab is an anti-GCC antibody molecule as described herein, and m has the values and preferred values described above for formula (1). 00 \N O N S MeO N HN Ab MoO HO 0 O Ab C \ NH MeO N 0 N-0 _ Mo HO H (1-2) 86 WO 2011/050242 PCTUS2010/053686 0 00A Cl \ O 0 0 'Ab MeO N O N O L. MeO HOH [0269] In some embodiments, the variable Ab in formula (I-1), (1-2), or (I-3) is an antibody molecule with features summarized in Tables 1 to 6. In certain embodiments, the variable Ab is a 5F9 antibody molecule or an Abx-229 antibody molecule. [0270] In some embodiments, the variable m in formula (I-), (I-2), or (I-3) ranges from about I to about 10, from about 3 to about 7, or from about 3 to about 5. [0271] In certain particular embodiments, the invention relates to an immunoconjugate of formula (I-1), (I-2), or (I-3), wherein Ab is a 5F9 antibody molecule and m is about 4. Dolastatin and auristatin immunoconjugates [02721 In some other embodiments the therapeutic agent is a dolastatin. In some embodiments, the therapeutic agent is an auristatin, such as auristatin E (also known in the art as a derivative of dolastatin-10) or a derivative thereof. In some embodiments, the therapeutic agent is a compound selected from compounds of formulae (X1I)-(XXII), or a pharmaceutically acceptable salt form thereof: H3C CH3 H3C H 0C3CH3 N NN I I N CH3 CH3 0 CH3 O)CH3 0 H H3C C3 OCH 0(bu 87 WO 2011/050242 PCT/US2010/053686
NH
2 00 H O NN O C'H3 0 OCH3 O H M N0 H HN(xv) NN 0 N O -OCH 3 O
OCH
3 O H (Xv)
H
3 C
CH
3 OH 3 C H N N N H3C H3 OCH 0 l N S
CH
3 OH3 CHCH OCH 3 O O H 0 H
CH
3 (XVI) Hl
H
2 N N N 4 ,,. N ' O 111OCH 3 HN7 0O f3 0OCH 3 0 H(XVII) HfC CH 3
H
3 C H 0 CuH0 N N N N 4 ,, N
CH
3 0 CH 3 OCH H HsC HR 3
OHOCH
3 0 (XVIII)
H
3 C
CH
3 Hf 3 C CI Hl
CH
3 HNN N s
CH
3 0 CH 3
OCH
3 0 0C 3 0H 88 WO 2011/050242 PCT/US2010/053686 HH N N NNN O OCH3 0 OC H3 0 H H 0 CH HN N 14 NN HN %1 0 N ON Mt (X") 0 NN I -N 0 OCH 3 O0 H oca 3 0 (XJ 0 0 0 0 ' H N,,. HN N N 0 0CH 3 0 I-f HP w l [0273] Auristatin compounds and methods for their conjugation to antibodies are described, for example, in Doronina et al., Nature Biotech., 21: 778-784 (2003); Hamblett et al, Clin. Cancer Res., 10: 7063-7070 (2004); Carter and Senter, Cancer J., 14 154-169 (2008); U.S. Patent Nos. 7,498,298, 7,091,186, 6,884,869; 6,323,315; 6,239,104; 6,034,065; 5,780,588; 5,665,860; 5,663,149; 5,635,483; 5,599,902; 5,554,725; 5,530,097; 5,521,284; 5,504,191; 5,410,024; 5,138,036; 5,076,973; 4,986,988; 4,978,744; 4,879,278; 4,816,444; and 4,486,414; U.S. Patent Publication Nos. 20090010945, 20060074008, 20080300192, 20050009751, 20050238649, and 20030083236; and International Patent 89 WO 2011/050242 PCT/US2010/053686 Publication Nos. WO 04/010957 and WO 02/088172, each of which is incorporated by reference herein in its entirety and for all purposes. [0274] The auristatin can be, for example, an ester formed between auristatin E and a keto acid. For example, auristatin E can be reacted with paraacetyl benzoic acid or benzoylvaleric acid to produce AEB and AEVB, respectively. Other typical auristatins include auristatin phenylalanine phenylenediamine (AFP; (XVHI)), monomethyl auristatin E (MMAE; (XTI)), and monomethyl auristatin F (MMAF; (KXW)). [0275] Auristatins have been shown to interfere with microtubule dynamics and nuclear and cellular division and have anticancer activity. Auristatins for use in the present invention bind tubulin and can exert a cytotoxic or cytostatic effect on a GCC expressing cell line. Methods for determining whether a compound binds tubulin are known in the art. See, for example, Muller et al., Anal. Chem 2006, 78, 4390-4397; Hamel et al., Molecular Pharmacology, 1995 47: 965-976; and Hamel et al., The Journal of Biological Chemistry, 1990 265:28, 17141-17149. For purposes of the present invention, the relative affinity of a compound to tubulin can be determined. Some preferred auristatins of the present invention bind tubulin with an affinity ranging from 10-fold lower (weaker affinity) than the binding affinity of MMAE to tubulin to 10-fold, 20-fold or even 100-fold higher (higher affinity) than the binding affinity of MMAE to tubulin. [0276] There are a number of different assays, known in the art, that can be used for determining whether an auristatin or resultant immunoconjugate exerts a cytostatic or cytotoxic effect on a desired cell line. For example, the cytotoxic or cytostatic activity of an immunoconjugate can be measured by: exposing mammalian cells expressing a target protein of the immunoconjugate in a cell culture medium; culturing the cells for a period from about 6 hours to about 5 days; and measuring cell viability. Cell-based in vitro assays can be used to measure viability (proliferation), cytotoxicity, and induction of apoptosis (caspase activation) of the immunoconjugate. [02771 For determining whether an immunoconjugate exerts a cytostatic effect, a thymidine incorporation assay may be used. For example, cancer cells expressing a target antigen at a density of 5,000 cells/well of a 96-well plated can be cultured for a 72-hour period and exposed to 0.5 RCi of 3 H-thymidine during the final 8 hours of the 72-hour period. The incorporation of 3 H-thymidine into cells of the culture is measured in the presence and absence of the immunoconjugate. 90 WO 2011/050242 PCT/US2010/053686 [0278] For determining cytotoxicity, necrosis or apoptosis (programmed cell death) can be measured. Necrosis is typically accompanied by increased permeability of the plasma membrane; swelling of the cell, and rupture of the plasma membrane. Apoptosis is typically characterized by membrane blebbing, condensation of cytoplasm, and the activation of endogenous endonucleases. Determination of any of these effects on cancer cells indicates that an immunoconjugate is useful in the treatment of cancers. [0279] Cell viability can be measured by determining in a cell the uptake of a dye such as neutral red, trypan blue, or ALAMARTM blue (see, e.g., Page et al., 1993, Intl. J. Oncology 3:473-476). In such an assay, the cells are incubated in media containing the dye, the cells are washed, and the remaining dye, reflecting cellular uptake of the dye, is measured spectrophotometrically. The protein-binding dye sulforhodamine B (SRB) can also be used to measure cytotoxicity (Skehan et al., 1990, J. Natl. Cancer Inst. 82:1107 12). [0280] Alternatively, a tetrazolium salt, such as MTT or WST, is used in a quantitative colorimetric assay for mammalian cell survival and proliferation by detecting living, but not dead, cells (see, e.g., Mosmann, 1983, J. limnunol. Methods 65:55-63). [02811 Apoptosis can be quantitated by measuring, for example, DNA fragmentation. Commercial photometric methods for the quantitative in vitro determination of DNA fragmentation are available. Examples of such assays, including TUNEL (which detects incorporation of labeled nucleotides in fragmented DNA) and ELISA-based assays, are described in Biochemica, 1999, no. 2, pp. 34-37 (Roche Molecular Biochemicals). [0282] Apoptosis can also be determined by measuring morphological changes in a cell. For example, as with necrosis, loss of plasma membrane integrity can be determined by measuring uptake of certain dyes (e.g., a fluorescent dye such as, for example, acridine orange or ethidium bromide). A method for measuring apoptotic cell number has been described by Duke and Cohen, Current Protocols in Immunology (Coligan et al. eds., 1992, pp. 3.17.1-3.17.16). Cells also can be labeled with a DNA dye (e.g., acridine orange, ethidium bromide, or propidium iodide) and the cells observed for chromatin condensation and margination along the inner nuclear membrane. Other morphological changes that can be measured to determine apoptosis include, e.g., cytoplasmic condensation, increased membrane blebbing, and cellular shrinkage. 91 WO 2011/050242 PCT/US2010/053686 [0283] The presence of apoptotic cells can be measured in both the attached and "floating" compartments of the cultures. For example, both compartments can be collected by removing the supernatant, trypsinizing the attached cells, combining the preparations following a centrifugation wash step (e.g., 10 minutes at 2000 rpm), and detecting apoptosis (e.g., by measuring DNA fragmentation). (See, e.g., Piazza et aL, 1995, Cancer Research 55:3110-16). [02841 The effects of immunoconjugates can be tested or validated in animal models. A number of established animal models of cancers are known to the skilled artisan, any of which can be used to assay the efficacy of an immunoconjugate. Non limiting examples of such models are described infra. Moreover, small animal models to examine the in vivo efficacies of immunoconjugates can be created by implanting human tumor cell lines into appropriate immunodeficient rodent strains, e.g., athynic nude mice or SCID mice. [02895 In some embodiments, the variable -Z in formula (1) is an auristatin moiety of the formula (X-A) or formula (X-B): R3 0 R 7
R
9 R20
R
2
R
4
R
5 R6 R1 (X-A)
R
8 0 R 21 R3 0
R
7 H R 9 0 ,]N N ,R1
R
2 0 R 4
R
5 R R 8 O G RB 0 (X-B) wherein, independently at each location: the wavy line indicates a bond;
R
2 is -C 1
-C
20 alkyl, -C 2
-C
20 alkenyl, or -C 2
-C
20 alkynyl; RW is -H, -C-C 20 alkyl, -C 2
-C
20 alkenyl, -C 2
-C
20 alkynyl, carbocycle, -C-C 20 alkylene (carbocycle), -C 2
-C
2 0 alkenylene(carbocycle), -C 2
-C
20 alkynylene(carbocycle), -aryl, CI-C 20 alkylene(aryl), -C 2
-C
20 alkenylene(aryl), -C 2
-C
2 0 alkynylene(aryl), -heterocycle,
-C-C
20 alkylene(heterocycle), -C 2
-C
2 0 alkenylene(heterocycle), or -C 2
-C
20 alkynylerie(heterocycle); 92 WO 2011/050242 PCTIUS2010/053686 R4 is -H, -C-C 20 alkyl, -Q-C 20 alkenyl, -C-C 20 alkynyl, carbocycle, -C-C 2 0 alkylene (carbocycle), -C 2
-C
20 alkenylene(carbocycle), -C 2
-C
20 alkynylene(carbocycle), -aryl, C-C 20 alkylene(aryl), -C-C2o alkenylene(aryl), -C 2
-C
20 alkynylene(aryl), -heterocycle,
-C
1
-C
20 alkylene(heterocycle), -C 2
-C
2 0 alkenylene(heterocycle), or -C 2
-C
20 alkynylene(heterocycle); Rt is -H or -C-Cs alkyl; or R and R 5 jointly fbrm a carbocyclic ring and have the formula -(CRaRe)- wherein Ra and Rb are independently -H, -C 1
-C
20 alkyl, -C 2
-C
20 alkenyl, -CrC2o alkynyl, or carbocycle and s is 2, 3, 4, 5 or 6, it is -H, -C-C 20 alkyl, -C 2
-C
2 0 alkenyl, or -C 2
-C
2 0 alkynyl; R7 is -H, -C-C 2 0 alkyl, -C 2
-C
20 alkenyl, -C 2
-C
20 alkynyl, -carbocycle, -C-C 20 alkylene (carbocycle), -C 2
-C
2 0 alkenylene(carbocycle), -C 2
-C
20 alkynylene(carbocycle), -aryl, C-C 20 alkylene(aryl), -C 2
-C
20 alkenylene(aryl), -C 2
-C
2 0 alkynylene(aryl), heterocycle,
-C-C
20 alkylene(heterocycle), -C 2
-C
20 alkenylene(heterocycle), or -C 2
-C
20 alkynylene(heterocycle); each R 8 is independently -H, -OH, -C-C 20 alkyl, -C 2
-C
20 alkenyl, -C 2
-C
2 o alkynyl, -O-(Ci
C
20 alkyl), -O-(C 2
-C
20 alkenyl), -O-(CI-C 2 0 alkynyl), or -carbocycle; Rt is -H, -C-C 20 alkyl, -C 2
-C
20 alkenyl, or -C 2
-C
20 alkynyl;
R
9 is -aryl, -heterocycle, or -carbocycle;
R
20 is -H, -C-C 20 alkyl, -C 2
-C
20 alkenyl, -C 2
-C
20 alkynyl, -carbocycle, -O-(C-C 2 0 alkyl),
-O-(C
2
-C
20 alkenyl), -O-(C 2
-C
2 6 alkynyl), or OR 8 wherein Ris is -H, a hydroxyl protecting group, or a direct bond where ORs represents =0;
R
21 is -H, -C-C 2 0 alkyl, -C 2
-C
2 0 alkenyl, or -C 2
-C
2 0 alkynyl, -aryl, -heterocycle, or -carbocycle; RO is -aryl or -heterocycle; G is -0-, -S-, -NH-, or -NR' 2 -, wherein R 2 is -C-C 20 alkyl, -C 2
-C
20 alkenyl, -C 2
-C
20 alkynyl; Rn is -H, -C-C 20 alkyl, -C 2
-C
20 alkenyl, -C 2
-C
2 0 alkynyl, -aryl, -heterocycle, -(R'O),-R 4 , or -(R"O)s-CH(Ri)k s is an integer ranging from 0-1000; R is -C 2
-C
20 alcylene, -C 2
-C
2 0 alkenylene, or -C 2
-C
20 alkynylene; i is -H, -C-C 20 alkyl, -C 2
-C
20 alkenyl, or -C 2
-C
20 alkynyl; 93 WO 2011/050242 PCT/US2010/053686 each occurrence of Rs is independently -H, -COOH, -(CH 2 )rN(R 6 )2, -(CH 2 )rSO3H,
-(CH
2 )rSO3-CIC 2 0 alkyl, -(CH 2 )1-SO 3
-C
2
-C
20 alkenyl, or -(CH 2
)-SO
3
-C
2
-C
2 0 alkynyl; each occurrence of R1is independently -H, -CrC 2 0 alkyl, -C 2
-C
2 0 alkenyl, -C 2
-C
20 alkynyl or -(CH 2 )t-COOH; and t is an integer ranging from 0 to 6; wherein said alkyl, alkenyl, alkynyl, alkylene, alkenylene, alkynyklene, aryl, carbocyle, and heterocycle radicals, whether alone or as part of another group, are optionally substituted. [0286] Auristatins of the formula (X-A) include those wherein said alkyl, alkenyl, alkynyl, alkylene, alkenylene, alkynyklene, aryl, carbocyle, and heterocycle radicals are unsubstituted. 10287] Auristatins of the formula (X-A) include those wherein the groups of R 2 , R3,
R
4 , R 5 , R, R7, R 8 , and R 9 are unsubstituted and the groups of R' 9 , R 20 and R 2 are optionally substituted as described herein. [0288] Auristatins of the formula (X-A) include those wherein R is -C-C 8 alkyl;
R
3 , R 4 and R 7 are independently selected from -H, -C-C 20 alkyl, -C2-C2 alkenyl,
-C
2
-C
20 alkynyl, monocyclic C3-C carbocycle, -CrC2o alkylene(monocyclic C 3
-C
6 carbocycle), -C 2 -C2 0 alkenylene(monocyclic C 3
-C
6 carbocycle), -C 2
-C
20 alkynylene(monocyclic C 3
-C
6 carbocycle), -C 6 -Cio aryl, -CI-C 20 alkylene(C 6 -Cio aryl), C 2
-C
2 0 alkenylene(C 6 -C1o aryl), -C 2
-C
2 0 alkynylene(C 6 -Cio aryl), -heterocycle, -C-C 2 0 alkylene(heterocycle), -C 2
-C
20 alkenylene(heterocycle), or -C 2
-C
2 0 alkynylene(heterocycle); wherein said alkyl, alkenyl, alkynyl, alkylene, alkenylene, alkynylene, carbocycle, aryl, and heterocycle radicals are optionally substituted; R is -hydrogen; R' is -C 1
-C
8 alkyl; each RR is independently selected from -OH, -O-(C-C 2 0 alkyl), -O-(C 2
-C
20 alkenyl), or -O-(C 2
-C
20 alkynyl) wherein said alkyl, alkenyl, and alkynyl radicals are optionally substituted; R is -hydrogen or -C-C 8 alkyl;
R
9 is optionally substituted phenyl; 94 WO 2011/050242 PCT/US2010/053686
R
20 is OR'; wherein R8 is H, a hydroxyl protecting group, or a direct bond where OR" represents =0; R21 is selected from -H, -C-C 2 o alkyl, -C 2
-C
2 0 alkenyl, -C 2
-C
20 alcynyl, or -carbocycle; wherein said alkyl, alkenyl, alkynyl, and carbocycle radicals are optionally substituted; or a pharmaceutically acceptable salt form thereof. [02891 Auristatins of the formula (X-A) include those wherein
R
2 is methyl;
R
3 is -H, -CI-C alkyl, -C 2 -Cs alkenyl, or -C 2
-C
2 alkynyl, wherein said alkyl, alkenyl and alkynyl radicals are optionally substituted;
R
4 is -H, -C-C 8 alkyl, -C 2
-C
8 alkCenyl, -C 2
-C
8 alkynyl, nonocyclic C 3
-C
6 carbocycle, -C 6
-C
10 aryl, -C-C 8 alkylene(C6-Cg aryl), -C 2 -Cs alkenylene(C 6 -Co aryl), C 2
-C
8 alkynylene(C 6 -Cio aryl), -C-Cs alkylene (monocyclic C 3
-C
6 carbocycle), -C 2
-C
8 alkenylene (monocyclic C 3
-C
6 carbocycle), -C 2
-C
8 alkynylene(monocyclic C 3
-C
6 carbocycle); wherein said alkyl, alkenyl, alkynyl, alkylene, alkenylene, alkynylene, aryl, and carbocycle radicals whether alone or as part of another group are optionally substituted; R is H; R 6 is methyl;
R
7 is -C-C 8 alkyl, -C 2 -Cs alkenyl or -C 2 -Cs alkynyl; each R is methoxy; R' is -hydrogen or -CrCs alkyl; R" is phenyl;
R
20 is OR"; wherein R' 8 is -H, a hydroxyl protecting group, or a direct bond where OR' represents =0;
R
1 is methyl; or a pharmaceutically acceptable salt form thereof. [0290] Auristatins ofthe formula (X-A) include those wherein
R
2 is methyl; R 3 is H or C-C 3 alkyl; R 4 is CrCs alkyl; R 5 is H; R 6 is methyl; R 7 is isopropyl or sec-butyl; R is methoxy; Re is hydrogen or C-C 8 alkyl; R" is phenyl; R 2 is OR"'; wherein R' is H, a hydroxyl protecting group, or a direct bond where OR 8 represents =0; and R 2 1 is methyl; or a pharmaceutically acceptable salt form thereof. [02911 Auristatins of the formula (X-4) include those wherein
R
2 is methyl or C-C 3 alkyl; R 3 is H or C 1
-C
3 alkyl; R4 is CI-Cs alkyl; R5 is H; R6 is C-C 3 alkyl; R 7 is C-C 5 alkyl; R 8 is C-C 3 alkoxy; R9 is hydrogen or C-C8 alkyl; R 1 9 is phenyl; 95 WO 2011/050242 PCTJUS201o/053686
R
2 is OR' 8 ; wherein R"1 is H, a hydroxyl protecting group, or a direct bond where OR 18 represents =0; and R2 is C-C 3 alkyl; or a pharmaceutically acceptable salt form thereof. [0292] Auristatins of the formula (X-B) include those wherein R2 is methyl;
R
3 , R4, and RW are independently selected from -H, -C 1
-C
2 0 alkyl, -C 2
-C
20 alkenyl, -C 2
-C
20 alkynyl, monocyclic C 3
-C
6 carbocycle, -C-C 20 alkylene(monocyclic C 3 C 6 carbocycle), -C 2
-C
20 alkenylene(monocyclic C 3 -Q carbocycle), -C 2
-C
20 alkynylene(monocyclic C 3
-C
6 carbocycle), -C 6
-C
10 aryl, -C-C 2 0 alkylene(C 6 -CiO aryl),
C
2
-C
20 alkenylene(C 6 -C10 aryl), -C 2
-C
20 alkynylene(C6-CIO aryl), -heterocycle, -C1-C20 alkylene(heterocycle), -C 2
-C
20 alkenylene(heterocycle), or -CrC20 alkynylene(heterocycle); wherein said alkyl, alkenyl, alkynyl, alkylene, alkenylene, alkynylene, carbocyle, aryl, and heterocyclic radicals whether alone or as part of another group are optionally substituted; RW is -H;
R
6 is methyl; each R is methoxy; R is -H, -CrC2o alkyl, -C 2 -C20 alkenyl, or -C-C 2 alkynyl; wherein said alkyl, alkenyl and alkynyl radical are optionally substituted; RO is optionally substituted aryl or optionally substituted heterocycle; G is -0-, -S-, -NH-, or -NR"-, wherein R is -C-C 20 alkyl, -C 2
-C
20 alkenyl, or C 2
-C
20 alkynyl, each of which is optionally substituted; R" is -H, -C-C 20 alkyl, -C 2
-C
20 alkenyl, -C 2
-C
20 alkynyl, -aryl, -heterocycle, -(R 3
O),-R
14 , or -(R 3 0),-CH(R') 2 , wherein said alkyl, alkenyl, alkynyl, aryl, and heterocycle radicals are optionally substituted; s is an integer ranging from 0-1000; R3 is -C 2
-C
20 alkylene, -C 2
-C
2 o alkenylene, or -C 2
-C
20 alkynylene, each of which is optionally substituted;
R
1 4 is -H, -C-C 2 0 alkyl, -C 2
-C
20 alkenyl, or -C 2
-C
20 ailcynyl wherein said alkyl, alkenyl and alkynyl radicals are optionally substituted; each occurrence of R 5 is independently -H, -COOH, -(CH2)t-N(R) 2 , -(CH 2 )r
SO
3 H, -(CH 2 )rSO 3
-C-C
2 0 alkyl, -(CH 2 )rSO 3
-C
2
-C
20 alkenyl, or -(CH 2 )rSO-CrC 2 0 alkynyl wherein said alkyl, alkenyl and alkynyl radicals are optionally substituted; 96 WO 2011/050242 PCT/US2010/053686 each occurrence of R' 6 is independently -H, -C1-C2o alkyl, -C 2
-C
20 alkenyl, -C 2
C
2 0 alkynyl or -(CH 2 )rCOOH wherein said alkyl, alkenyl and alkynyl radicals are optionally substituted; t is an integer ranging from 0 to 6; or a pharmaceutically acceptable salt form thereof [0293] In certain of these embodiments, R') is optionally substituted phenyl; [0294] Auristatins ofthe formula (X-B) include those wherein the groups of R 2 , R,
R
4 , R 5 , R6, R7, R 8 , and R9 are unsubstituted and the groups of R1 0 and R" are as described herein. [02951 Auristatins of the formula (X-B) include those wherein said alkyl, alkenyl, alkynyl, alkylene, alkenylene, alkynyklene, aryl, carbocyle, and heterocycle radicals are unsubstituted. [02961 Auristatins ofthe formula (X-B) include those wherein
R
2 is C 1
-C
3 alkyl; R 3 is H or C 1
-C
3 alkyl; R 4 is C 1
-C
5 alkyl; R is H; R' is C 1
-C
3 alkyl; Rt is CE-Cs alkyl; R 8 is C 1
-C
3 alkoxy; R 9 is hydrogen or C 1 -Cs alkyl; R" is optionally substituted phenyl; G is 0, S, or NH; and R" 1 is as defined herein; or a pharmaceutically acceptable salt form thereof. [0297] Auristatins of the formula (X-B) include those wherein
R
2 is methyl; R3 is H or C 1
-C
3 alkyl; R 4 is C 1 -Cs alkyl; R 5 is H; R6 is methyl; R 7 is isopropyl or sec-butyl; R is methoxy; l is hydrogen or C 1
-C
8 alkyl; R" is optionally substituted phenyl; G is 0, S, or NH; and Rn is as defined herein; or a pharmaceutically acceptable salt form thereof [0298] Auristatins of the formula (X-B) include those wherein R2 is methyl; R is H or C 1
-C
3 alkyl; R 4 is C 1
-C
5 alkyl; R is H; R 6 is methyl; R 7 is isopropyl or sec-butyl; R 8 is methoxy; R! is hydrogen or C 1
-C
8 alkyl; RO is phenyl; and G is 0 or NH and R" is as defined herein, preferably hydrogen; or a pharmaceutically acceptable salt form thereof [02991 Auristatins of the formula (X-B) include those wherein
R
2 is C 1
-C
3 alkyl; R 3 is H or CI-C 3 alkyl; R4 is C 1
-C
5 alkyl; R is H; R6 is C 1
-C
3 alkyl; R 7 is C 1 -Cs alkyl; R 8 is CI-C 3 alkoxy; R' is hydrogen or Ci-Cs alkyl; 97 WO 2011/050242 PCT/US2010/053686 R is phenyl; and G is 0 or NH and R" is as defined herein, preferably hydrogen; or a pharmaceutically acceptable salt form thereof. [0300] Auristatins of the formula (X-A) or (X-B) include those wherein RW, R 4 and R' are independently isopropyl or sec-butyl and R 5 is -H. In an exemplary embodiment, R3 and R4 are each isopropyl, R 5 is H, and R7 is sec-butyl. The remainder of the substituents are as defined herein. [0301] Auristatins of the formula (X-A) or (X-B) include those wherein R2 and R6 are each methyl, and R9 is H. The remainder of the substituents are as defined herein. [0302] Auristatins of the formula (X-A) or (X-B) include those wherein each occurrence of R8 is -OCH 3 . The remainder of the substituents are as defined herein. 10303] Auristatins of the formula (X-A) or (X-B) include those wherein RW and R4 are each isopropyl, R2 and R6 are each methyl, R is H, R7 is sec-butyl, each occurrence of R is -OCH 3 , and R9 is H. The remainder of the substituents are as defined herein. [03041 Auristatins of the formula (X-B) include those wherein G is -0- or -NH-. The remainder of the substituents are as defined herein. [0305] Auristatins of the formula (X-B) include those wherein R"1 is aryl. The remainder of the substituents are as defined herein. [0306] Auristatins of the formula (X-B) include those where R10 is -phenyl. The remainder of the substituents are as defined herein. [0307] Auristatins of the formula (X-B)include those wherein G is -0-, and R" is H, methyl or t-butyl. The remainder of the substituents are as defined herein. [03081 Auristatins of the formula (X-B) include those wherein, when G is -NH, R1 is -(R0),-CH(R 5
)
2 , wherein R15 is -(CH 2
),-N(R)
2 , and R' is -C 1 -Cs alkyl or -(CH2)r COOH. The remainder of the substituents are as defined herein. [0309] Auristatins of the formula (X-B) include those wherein when G is -NH, R" is -(R 3 0),-CH(R 5
)
2 , wherein R1 is H or -(CH2)rSO3H. The remainder of the substituents are as defined herein. [03101 In preferred embodiments of the immunoconjugates of formula (I), when Z is an auristatin molecule of formula (X-A), w is an integer ranging from I to 12, preferably 2 to 12, y is 1 or 2, and a is preferably 1. 98 WO 2011/050242 PCT/US2010/053686 [03111 In some embodiments of the immunoconjugates of formula (D), when Z is an auristatin molecule of formula (X-B), a is I and w and y are 0. [0312] Illustrative therapeutic agents (-Z) include those having the following structures: oNH >H H 0
CH
3 O N NH H 0OCH 3 0 OCH 3 0 QJH OH N , N 9 O OH 4 C99 WO 2011/050242 PCT/US2010/053686 NN H. O O O OCHsO0 0HOCH 3 O
C
3 NN H H N N N 0 7 H N H N N' N'N N
I
0 N. 0 >2t0N 1 0 N N N N0 y N 0
OCH
3 O0 OH 3 o H HH
N
11 H /N' N ';:FN YN I 0 0
-
0 0 100 WO 2011/050242 PCT/US2010/053686 / HO HOOCN, I N oCOOH S
-
HOC, O~.OOH ,n N~ N: 0 NH2 S0 3 H 001 0 0O-O 0O 0-:J:INH<) HOOC -I COOH ,and H 0 4 H
NH
2 f03131 in one aspect, hydrophilic groups, such as but -not limited to triethylene glycol esters (TEG) can be attached to the auristatin molecule of formula (X-B) at R". Without being bound by theory, the hydrophilic groups assist in the internalization and non-agglomeration of the therapeutic agent. 101 WO 2011/050242 PCT/US2010/053686 [03141 In some embodiments, the therapeutic agent is not TZT-1027. In some embodiments, the therapeutic agent is not auristatin E, dolastatin 10, or auristatin PE. [0315] In some embodiments, the auristatin molecule is linked to a cysteine moiety on the antibody molecule by way of a linker containing a maleimide moiety, e.g., a maleimidocaproyl moiety. [0316] In some embodiments, the auristatin molecule is coupled to the antibody molecule using a heterobifunctional linker that is connected to a hydroxyl group on the auristatin molecule. In some such embodiments, the linker comprises a hydrazone. In some embodiments, the linker is a hydrazone compound formed by reaction of maleimidocaproylhydrazide and a ketocarboxylic acid, e.g., 5-benzoylvaleric acid. In particular embodiments, the linker is (Z)-6-(2-(6-(2,5-dioxo-2,5-dihydro-1H-pyrrol- 1 -yl) hexanoyl)hydrazono)-6-phenylhexanoic acid. [0317] In some other embodiments the auristatin molecule is coupled to the antibody using a heterobifunctional linker that is connected to a monomethyl amino group on the auristatin molecule. In some embodiments, the linker comprises a cleavable moiety, e.g., a peptide moiety, and a self-imnimolativep-aminobenzylcarbamate spacer. Exemplary linkers include maleimidocaproyl (mc), maleimidocaproyl-L-phenylalanine-L lysine-p-aminobenzylcarbamate, and maleimidocaproyl-L-valine-L-citrulline-p aminobenzylcarbamate (vc). [0318] In certain embodiments, the immunoconjugate of formula (1) is characterized by the formula Ab-(vc-MMAF). (formula (1-4)); Ab-(vc-MMAE). (formula (1-5)); Ab-(mc-MMAE). (formula (1-6)); or Ab-(mc-MMAF), (formula (1-7)), wherein Ab is an anti-GCC antibody molecule as described herein, S is a sulfur atom of the antibody, and m has the values and preferred values described above for formula (1). In certain embodiments, m is an integer from I to about 5. 4to,% N% N rN O %0 A OX >NH
H
2 N'&O 102 10 WO 2011/050242 PCTfUS2010/053686 >NH Ab HN
H
2 N J.O Ab 1 Ab N N L M (1-7) [0319] In some embodiments, the variable Ab in formula (1-4), (I-5), (I-6), or (I-7) is an antibody molecule with features summarized in Tables 1 to 6. In certain embodiments, the variable Ab is a 5F9 antibody molecule or an Abx-229 antibody molecule. [0320] In some embodiments, the variable in in formula (I-4), (I-5), (I-6), or (1-7) ranges from about 2 to about 10, from about 6 to about 8, or frm about 4 to about 6. [0321] In certain particular embodiments, the invention relates to an immunoconjugate of formula (I-4), (I-5), (I-6), or (I-7), wherein Ab is a SF9 antibody molecule and in is about 4. [0322] The immunoconjugates disclosed herein can be used for modifying a given biological response. The therapeutic agent is not to be construed as limited to classical chemical therapeutic agents. For example, the therapeutic agent may be a nucleic acid, protein, or polypeptide possessing a desired biological activity. For example, the antibody 103 WO 2011/050242 PCT/US2010/053686 molecule can be conjugated to an antisense molecule, an siRNA molecule, shRNA molecule or miRNA molecule that can interfere with expression of a gene, thereby producing a desired biological effect. [0323] Proteins and polypeptides that can be conjugated to the antibody molecules of the invention include, for example, toxins and components thereof, such as abrin, abrin A chain, ricin, ricia A chain, modeccin, modeccin A chain, alpha-sarcin, exotoxin A (from Pseudomonas aeruginosa), PE38 (truncated pseudomonas exotoxin), gelonin, diphtheria toxin, diphtheria toxin A fragment, certain Aleuritesfordii proteins, certain Dianthus caryophyllus proteins (e.g., dianthin 30 and dianthin 32), certain Phytolacca Americana proteins (e.g., PAP, PAPII, and PAP-S), certain Saponaria officinlis proteins (e.g., saporin 6), Momordica charantia inhibitor, curcin, crotin, mitogillin, restrictocin, phenomycin, and enomycin; proteins to engage the immune system at the tumor or induce an effector function at the tumor, such as tumor necrosis factor, interferon, nerve growth factor, platelet derived growth factor, and tissue plasminogen activator; and biological response modifiers such as, for example, cytokines and lymphokines (e.g., interleukin-1 ("IL-1"), interleukin-2 ("IL-2"), interleukin-6 ("IL-6"), granulocyte macrophase colony stimulating factor ("GM-CSF"), and granulocyte colony stimulating factor ("G-CSF")), and other growth factors. [0324] The antibodies of the invention can also be conjugated or fused to viral surface proteins present on viral particles. For example, a single-chain anti-GCC antibody of the present invention could be fused (e.g., to form a fusion protein) to a viral surface protein. Alternatively, a whole anti-GCC antibody of the present invention, or a fragment thereof, could be chemically conjugated (e.g., via a chemical linker) to a viral surface protein. Preferably, the virus is one that fuses with endocytic membranes, e.g., an influenza virus, such that the virus is internalized along with the anti-GCC antibody and thereby infects GCC-expressing cells. The virus can be genetically engineered as a cellular toxin. For example, the virus could express or induce the expression of genes that are toxic to cells, e.g., cell death promoting genes. Preferably, such viruses would be incapable of viral replication. [0325] An anti-GCC antibody molecule described herein can also be conjugated to a prodrug or prod-rug activator. In a method to kill or suppress tumor cells, a first anti GCC antibody molecule of the invention is conjugated with a prodrug that is activated 104 WO 2011/050242 PCT/US2010/053686 only when in close proximity with a prodrug activator. The prodrug activator is conjugated with a second antibody molecule, preferably one that binds to a non-competing site on the GCC molecule. Whether two antibodies bind to competing or non-competing binding sites can be determined by conventional competitive binding assays. Drug-prodrug pairs suitable for use in the practice of the present invention are described in Blakely et at., "ZD2767, an Improved System for Antibody-directed Enzyme Prodrug Therapy That Results in Tumor Regressions in Colorectal Tumor Xenografts," (1996) Cancer Research, 56:3287-3292. [0326] Therapeutically active radioisotopes can also be coupled to anti-GCC antibodies, or antigen binding fragments, or derivatives thereof. Radioactive isotopes can be used in diagnostic or therapeutic applications. Radioactive isotopes that can be coupled to the anti-GCC antibodies include, but are not limited to a-, s-, or y-emitters, or P- and y emitters. See, e.g., S.E. Order, "Analysis, Results, and Future Prospective of the Therapeutic Use of Radiolabeled Antibody in Cancer Therapy," MonoclonaltAntibodies for Cancer Detection and Therapy, R.W. Baldwin et al. (eds.), pp 303-316 (Academic Press 1985. Such radioactive isotopes include, but are not limited to copper ( 64 Cu), iodine (1311 or 1251), yttrium ( 0 y), lutetium (.
7 Lu), actinium (Ac), praseodymium, astatine ("At), rhenium ( 1 86 Re), bismuth (1 12 Bi or 2 13 Bi), indium ("In), technetium ("mTc), phosphorus ( 32 P), rhodium ( 1 "Rh), sulfur ( 5 S), carbon (' 4 C), tritium ( 3 H), chromium
('
1 Cr), chlorine ( 36 C1), cobalt ("Co or 5 8 Co), iron ( 59 Fe), selenium ("Se), or gallium ( 7 Ga). Radioisotopes useful as therapeutic agents include yttrium ("Y), lutetium ("'Lu), actinium (m 5 Ac), praseodymium, astatine (2'At), rhenium ( 1 6 Re), bismuth (2Bi or 23Bi), and rhodium ('."Rh). Radioisotopes useful as labels, e.g., for use in diagnostics, include iodine (1311 or 1251), indium ("In), technetium ( 9 mTc), phosphorus ( 32 P), carbon ("C), and tritium (3H), or one or more of the therapeutic isotopes listed above. [0327] Radioimmunotherapy (RIT) using antibodies labeled with 131 i, 1Y, and "7Lu is under intense clinical investigation. There are significant differences in the physical characteristics of these three nuclides and as a result, the choice of radionuclide can be important in order to deliver maximum radiation dose to the tumor. The higher beta energy particles of 90 Y may be good for bulky tumors, but it may not be necessary for small tumors and especially bone metastases. The relatively low energy beta particles of m'I are ideal, but in vivo dehalogenation of radioiodinated molecules is a major disadvantage for internalizing antibody. In contrast, 177 Lu has low energy beta particle 105 WO 2011/050242 PCT/US2010/053686 with only 0.2-0.3 mm range and delivers much lower radiation dose to bone marrow compared to 90 Y. In addition, due to longer physical half-life (compared to "'Y), the tumor residence times are higher. As a result, higher activities (more mCi amounts) of 177 Lu labeled agents can be administered with comparatively less radiation dose to marrow. There have been several clinical studies investigating the use of 177 Lu labeled antibodies in the treatment of various cancers. (Mulligan T et al. Clin Cancer Res. 1: 1447-1454(1995); Meredith RF, et al. JNuclMed 37:1491-1496(1996); Alvarez RD, et al. Gynecologic Oncology 65: 94-101(1997)). [0328] Useful detectable agents with which an antibody or an antibody portion of the invention may be derivatized (or labeled) include fluorescent compounds, various enzymes, prosthetic groups, luminescent materials, bioluminescent materials, fluorescent emitting metal atoms, e.g., europium (Eu), and other anthanides, and radioactive materials (described above). Exemplary fluorescent detectable agents include fluorescent, fluorescein isothiocyanate, rhodamine, 5-dimethylamine-1-napthalenesulfonyl chloride, phycoerythrin and the like. An antibody may also be derivatized with detectable enzymes, such as alkaline phosphatase, horseradish peroxidase, p-galactosidase, acetylcholinesterase, glucose oxidase and the like. When an antibody is derivatized with a detectable enzyme, it is detected by adding additional reagents that the enzyme uses to produce a detectable reaction product. For example, when the detectable agent horseradish peroxidase is present, the addition of hydrogen peroxide and diaminobenzidine leads to a colored reaction product, which is detectable. An antibody may also be derivatized with a prosthetic group (e.g., streptavidin/biotin and avidin/biotin). For example, an antibody may be derivatized with biotin, and detected through indirect measurement of avidin or streptavidin binding. Examples of suitable fluorescent materials include umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; an example of a luminescent material includes luminol; and examples of bioluminescent materials include luciferase, luciferin, and aequorin. Pharmaceutical Compositions [0329] In another aspect, the invention features compositions, e.g., pharmaceutically acceptable compositions, which include an anti-GCC antibody molecule or immunoconjugate thereof, as described herein, formulated together with a 106 WO 2011/050242 PCT/US2010/053686 pharmaceutically acceptable carrier. In embodiments, the anti-GCC antibody molecule is one with exemplary features summarized in Tables 1 and 2. [0330] As used herein, "pharmaceutically acceptable carrier" includes any and all solvents, dispersion media, isotonic and absorption delaying agents, and the like that are physiologically compatible. The carrier can be suitable for intravenous, intramuscular, subcutaneous, parenteral, rectal, spinal or epidermal administration (e.g., by injection or infhsion). The pharmaceutical composition can include one or more additional excipients, e.g., salts, buffers, tonicity modifiers, lyoprotectants, nonionic detergents, surfactants, and preservatives. [0331] The compositions may be in a variety of forms. These include, for example, liquid, semi-solid and solid dosage forms, such as liquid solutions (e.g., injectable and infusible solutions), dispersions or suspensions, liposomes and suppositories. The preferred form depends on the intended mode of administration and therapeutic application. Some typical compositions are in the form of injectable or infusible solutions, intended for parenteral administration (e.g., intravenous, subcutaneous, intraperitoneal, intramuscular). In some embodiments, the antibody is administered by intravenous infusion or injection. In other embodiments, the antibody is administered by intramuscular or subcutaneous injection. [0332] The phrases "parenteral administration" and "administered parenterally" as used herein means modes of administration other than enteral and topical administration, usually by injection, and includes, without limitation, intravenous, intramuscular, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intraarticular, subcapsular, subarachnoid, intraspinal, epidural and intrasternal injection and infusion. [0333] In some embodiments, the pharmaceutical composition is sterile and stable under the conditions of manufacture and storage. The composition can be formulated as a solution, microemulsion, dispersion, liposome, microsphere, or other ordered structure suitable to high antibody concentration. Sterile injectable solutions can be prepared by incorporating the active compound (i.e., antibody or antibody portion) in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by sterilization, e.g., by filtration. Generally, dispersions are prepared by incorporating the active compound into a sterile vehicle that contains a basic 107 WO 2011/050242 PCT/US2010/053686 dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the provided methods of preparation are vacuum drying and freeze-drying that yields a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof. The proper fluidity of a solution can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. Prolonged absorption of injectable compositions can be brought about by including in the composition an agent that delays absorption, for example, monostearate salts and gelatin. [0334] The antibodies and antigen binding fragments of the present invention can be administered by a variety of methods known in the art, although for many therapeutic applications, the route/mode of administration is intravenous injection or infusion. As will be appreciated by the skilled artisan, the route and/or mode of administration will vary depending upon the desired results. In certain embodiments, the active compound may be prepared with a carrier that will protect the compound against rapid release, such as a controlled release formulation, including implants, transdermal patches, and microencapsulated delivery systems. Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Many methods for the preparation of such formulations are patented or generally known to those skilled in the art. See, e.g., Sustained and Controlled Release Drug Delivery Systems, J.R. Robinson, ed., Marcel Dekker, Inc., New York, 1978. [0335] In certain embodiments, an anti-GCC antibody molecule or immunoconjugate described herein may be orally administered, for example, with an inert diluent or an assimilable edible carrier. The compound (and other ingredients if desired) may also be enclosed in a hard or soft shell gelatin capsule, compressed into tablets, buccal tablets, troches, capsules, elixirs, suspensions, syrups, wafers, and the like. To administer an antibody or an antibody fragment of the invention by other than parenteral administration, it may be necessary to coat the compound with, or co-administer the compound with, a material to prevent its inactivation. [03361 Therapeutic compositions can be administered with medical devices known in the art. For example, pharmaceutical preparations can be disposed within a device, e.g., 108 WO 2011/050242 PCTJS2010/053686 an air- or liquid-tight container, which contains one or more dosages. Examples of delivery devices include, without limitation, vials, cannulas, needles, drip bags, and lines. The invention also provides methods of placing an antibody molecule or immunoconjugate described herein into such a device. [0337] In some embodiments, the invention provides an anti-GCC antibody molecule or immunoconjugate described herein, which is formulated in a liposome composition. In some embodiments, the liposome is coated with antibody molecule. In some such embodiments, the liposome is filled with a therapeutic agent. Liposomic delivery can allow for the delivery of an agent, e.g., a therapeutic agent, that is not linked to the antibody. This approach can be used to deliver an agent, e.g., a therapeutic agent, that is not amenable to cross-linking to the antibody molecule or an agent, e.g., a therapeutic agent, which is to be sequestered, or which contact with non-target cells should be minimized. In particular embodiments, the liposome is filled with a cytostatic or cytotoxic agent. In certain particular embodiments, the therapeutic agent is selected from the group consisting of maytansinoids, an auristatins, dolastatins, duocarmycins, cryptophycins, taxanes, DNA alkylating agents calicheamicins, and derivatives of the foregoing. In other embodiments, the liposome is filled with nucleic acid sequence comprising RNA interference molecules, e.g., antisense molecules, siRNA, hsRNA or miRNA molecules, which are capable of diminishing GCC expression or the expression of another gene, e.g., an oncogene, in cells expressing GCC. In some other embodiments, the liposome is coated or filled with an immunoconjugate comprising an anti-GCC antibody molecule and a therapeutic agent or label. [03381 Dosage regimens are adjusted to provide the optimum desired response (e.g., a therapeutic response). For example, a single bolus may be administered, several divided doses may be administered over time or the dose may be proportionally reduced or increased as indicated by the exigencies of the therapeutic situation. It is especially advantageous to formulate parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. The term "dosage unit form," as used herein, refers to physically discrete units suited as unitary dosages for the subjects to be treated; each unit contains a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. The specification for the dosage unit forms of the invention are dictated by and directly dependent on (a) the unique characteristics of the active compound and the particular 109 WO 2011/050242 PCT/US2010/053686 therapeutic effect to be achieved, and (b) the limitations inherent in the art of compounding such an active compound for the treatment of sensitivity in individuals. [0339] An exemplary, non-limiting range for a therapeutically or prophylactically effective amount of an antibody or an antigen binding fragment of the invention is 0.1 20 mg/kg, or 1-10 mg/kg. It is to be noted that dosage values may vary with the type and severity of the condition to be alleviated. It is to be further understood that for any particular subject, specific dosage regimens should be adjusted over time according to the individual need and the professional judgment of the person administering or supervising the administration of the compositions, and that dosage ranges set forth herein are exemplary only and are not intended to limit the scope or practice of the claimed composition. [0340] The pharmaceutical compositions of the invention may include a "therapeutically effective" amount of an antibody or an antigen binding fragment of the invention. A "therapeutically effective" amount refers to an amount effective, at dosages and for periods of time necessary, to achieve the desired therapeutic result. A therapeutically effective amount of the modified antibody or antibody fragment may vary according to factors such as the disease state, age, sex, and weight of the individual, and the ability of the antibody or antibody portion to elicit a desired response in the individual. A therapeutically effective amount is also one in which any toxic or detrimental effects of the modified antibody or antibody fragment is outweighed by the therapeutically beneficial effects. A "therapeutically effective dosage" preferably inhibits a measurable parameter (e.g., tumor growth rate) in treated subjects by at least about 20%, at least about 40%, at least about 60%, and in some embodiments at least about 80%, relative to untreated subjects. The ability of a compound to inhibit a measurable parameter, e.g., cancer, can be evaluated in an animal model system predictive of efficacy in human tumors. Alternatively, this property of a composition can be evaluated by examining the ability of the compound to inhibit, such inhibition in vitro by assays known to the skilled practitioner. [0341] Also within the scope of the invention are kits comprising an anti-GCC antibody molecule or immunoconjugate as described herein. Further included are kits comprising liposome compositions comprising an anti-GCC antibody molecule or immunoconjugate. The kit can include one or more other elements including: instructions 110 WO 2011/050242 PCT/US2010/053686 for use; other reagents, e.g., a label, a therapeutic agent, or an agent useful for chelating, or otherwise coupling, an antibody to a label or therapeutic agent, or a radioprotective composition; devices or other materials for preparing the antibody for administration; pharmaceutically acceptable carriers; and devices or other materials for administration to a subject. Instructions for use can include instructions for diagnostic applications of the anti-GCC antibody molecule or immunoconjugate to detect GCC, in vitro, e.g., in a sample, e.g., a biopsy or cells from a patient having a cancer, or in vivo. The instructions can include guidance for therapeutic application including suggested dosages and/or modes of administration, e.g., in a patient with a cancer (e.g., a cancer of gastrointestinal origin, such as, for example, colon cancer, stomach cancer, esophageal cancer). Other instructions can include instructions on coupling of the antibody to a chelator, a label or a therapeutic agent, or for purification of a conjugated antibody, e.g., from unreacted conjugation components. As discussed above, the kit can include a label, e.g., any of the labels described herein. As discussed above, the kit can include a therapeutic agent, e.g., a therapeutic agent described herein. In some applications the antibody will be reacted with other components, e.g., a chelator or a label or therapeutic agent, e.g., a radioisotope, e.g., yttrium or lutetium. In such cases the kit can include one or more of a reaction vessel to carry out the reaction or a separation device, e.g., a chromatographic column, for use in separating the finished product from starting materials or reaction intermediates. [0342] The kit can further contain at least one additional reagent, such as a diagnostic or therapeutic agent, e.g., a diagnostic or therapeutic agent as described herein, and/or one or more additional anti-GCC antibody molecules or immunoconjugates, formulated as appropriate, in one or more separate pharmaceutical preparations. [0343] The kit can further contain a radioprotectant. The radiolytic nature of isotopes, e.g., 90 Yttrium ( 0 Y) is known. In order to overcome this radiolysis, radioprotectants may be included, e.g., in the reaction buffer, as long as such radioprotectants are benign, meaning that they do not inhibit or otherwise adversely affect the labeling reaction, e.g., of an isotope, such as of 90 Y, to the antibody. The formulation buffer of the present invention may include a radioprotectant such as human serum albumin (HSA) or ascorbate, which minimize radiolysis due to yttrium or other strong radionuclides. Other radioprotectants are known in the art and can also be used in the formulation buffer of the present invention, i.e., free radical scavengers (phenol, sulfites, 111 WO 2011/050242 PCT/US2010/053686 glutathione, cysteine, gentisic acid, nicotinic acid, ascorbyl palmitate, HOP(:O)H 2 1 glycerol, sodium formaldehyde sulfoxylate, Na 2
S
2 0., Na 2
S
2 0 3 , and SO 2 , etc.). [03441 A provided kit is one useful for radiolabeling a chelator- conjugated protein or peptide with a therapeutic radioisotope for administration to a patient. The kit includes (i) a vial containing chelator-conjugated antibody, (ii) a vial containing formulation buffer for stabilizing and administering the radiolabeled antibody to a patient, and (iii) instructions for performing the radiolabeling procedure. The kit provides for exposing a chelator-conjugated antibody to the radioisotope or a salt thereof for a sufficient amount of time under amiable conditions, e.g., as recommended in the instructions. A radiolabeled antibody having sufficient purity, specific activity and binding specificity is produced. The radiolabeled antibody may be diluted to an appropriate concentration, e.g., in formulation buffer, and administered directly to the patient with or without further purification. The chelator- conjugated antibody may be supplied in lyophilized form. Uses [03451 The anti-GCC antibody molecules described herein have in vitro and in vivo diagnostic, prognostic, imaging, therapeutic and prophylactic utilities. For example, these antibody molecules can be administered to cells in culture, e.g. in vitro or ex vivo, or administered in a subject, e.g., in vivo, to treat, prevent, and/or diagnose a variety of disorders. [03461 The antibody molecules, immunoconjugates, and fusion proteins described herein can be used can modulate an activity or function of a GCC protein, such as ligand binding (e.g., binding of ST or guanylin), GCC-mediated signal transduction, maintenance of intestinal fluid, electrolyte homeostasis, intracellular calcium release (calcium flux), cell differentiation, cell proliferation, or cell activation. [03471 In one aspect, the invention features a method of killing, inhibiting or modulating the growth of, or interfering with the metabolism of, a GCC-expressing cell. In one embodiment, the invention provides a method of inhibiting GCC-mediated cell signaling or a method of killing a cell. The method may be used with any cell or tissue which expresses GCC, such as a cancerous cell (e.g., a cell from a cancer of the gastrointestinal system, such as, for example, a cancer of the colon, stomach, or esophagus, or a pancreatic cell), or a metastatic lesion. Nonlimiting examples of GCC-expressing 112 WO 2011/050242 PCT/US2010/053686 cells include T84 human colonic adenocarcinoma cells, fresh or frozen colonic tumor cells, and cells comprising a recombinant nucleic acid encoding GCC or a portion thereof. [03481 Methods of the invention include the steps of contacting the cell with an anti-GCC antibody molecule or immunoconjugate thereof, as described herein, in an effective amount, i.e., amount sufficient to inhibit GCC-mediated cell signaling or an amount sufficient to kill the cell. The method can be used on cells in culture, e.g. in vitro, in vivo, ex vivo, or in situ. For example, cells that express GCC (e.g., cells collected by biopsy of a tumor or metastatic lesion; cells from an established cancer cell line; or recombinant cells), can be cultured in vitro in culture medium and the contacting step can be effected by adding the anti-GCC antibody molecule or immunoconjugate to the culture medium. In methods of killing a cell, the method comprises using a naked anti-GCC antibody molecule, or an immunoconjugate comprising an anti-GCC antibody molecule and a cytotoxic agent. The method will result in killing of cells expressing GCC, including in particular tumor cells expressing GCC (e.g., colonic tumor cells). [0349] Reference to Table 7 can serve as a guide to select an antibody(ies) to use for various methods. For example, Table 7 indicates which antibodies were confirmed to internalize after binding GCC. Such antibodies would be useful when linked to a cytotoxic moiety or a moiety for cell imaging. Antibodies which do not internalize can be used for diagnostic purposes or therapeutic methods using naked antibody designed to elicit an antibody-dependent cell-mediated cytotoxic response, or perhaps for liposome delivery methods. [0350J Anti-GCC antibody molecules of the present invention bind to extracellular domains of GCC or portions thereof in cells expressing the antigen. As a result, when practicing the methods of the present invention to kill, suppress, or detect cancerous cells, the antibodies or antigen binding fragments, bind to all such cells, not only to cells which are fixed or cells whose intracellular antigenic domains are otherwise exposed to the extracellular environment. Consequently, binding of the antibodies or antigen binding fragments, is concentrated in areas where there are cells expressing GCC, irrespective of whether these cells are fixed or unfixed, viable or necrotic. Additionally or alternatively, the anti-GCC antibody molecules, bind to and are internalized with GCC upon binding cells expressing the antigen. 113 WO 2011/050242 PCT/US2010/053686 [0351] The method also can be performed on cells present in a subject, as part of an in vivo protocol. In one embodiment, the subject is a human subject. Alternatively, the subject can be a mammal expressing a GCC antigen with which an anti-GCC antibody molecule disclosed herein cross-reacts. An anti-GCC antibody molecule or immunoconjugate thereof can be administered to a human subject for therapeutic purposes. An anti-GCC antibody molecule or immunoconjugate also can be administered to a non human mammal expressing the GCC-like antigen with which the antibody cross-reacts (e.g., a primate, pig or mouse) for veterinary purposes or as an animal model of human disease. Animal models may be useful for evaluating the therapeutic efficacy of antibodies of the invention (e.g., testing of dosages and time courses of administration). For in vivo embodiments, the contacting step is effected in a subject and includes administering an anti-GCC antibody molecule or immunoconjugate thereof to the subject under conditions effective to permit both binding of the antibody molecule to the extracellular domain of GCC expressed on the cell, and the treating of the cell. [03521 In one embodiment, the invention provides a method of treating cancer by administering an anti-GCC antibody molecule or an immunoconjugate comprising an anti GCC antibody molecule and a cytotoxic agent to a patient in need of such treatment. The method can be used for the treatment of any cancerous disorder which includes at least some cells that express the GCC antigen. As used herein, the term "cancer" is meant to include all types of cancerous growths or oncogenic processes, metastatic tissues or malignantly transformed cells, tissues, or organs, irrespective ofhistopathologic type or stage of invasiveness. The terms "cancer" and "tumor" may be used interchangeably (e.g., when used in the context of treatment methods, "treatment of a cancer" and "treatment of a tumor" have the same meaning). [0353] In embodiments, the treatment is sufficient to reduce or inhibit the growth of the subject's tumor, reduce the number or size of metastatic lesions, reduce tumor load, reduce primary tumor load, reduce invasiveness, prolong survival time, or maintain or improve the quality of life. [03541 Examples of cancerous disorders include, but are not limited to, solid tumors, soft tissue tumors, and metastatic lesions. Examples of solid tumors include malignancies, e.g., sarcomas, adenocarcinomas, and carcinomas, of the various organ systems, such as those affecting colon and pancreas. Adenocarcinomas include 114 WO 2011/050242 PCT/US2010/053686 malignancies such as non-small cell carcinoma of the lung, Metastatic lesions of the aforementioned cancers can also be treated or prevented using the methods and compositions of the invention. In some embodiments, the cancer to be treated is a cancer of the gastrointestinal system (e.g., colorectal cancer, esophageal cancer, or stomach cancer). In some embodiments, the pancreatic cancer. [0355] In one embodiment, the cancer is a colorectal cancer, e.g., colorectal adenocarcinoma, colorectal leiomyosarcoma, colorectal lymphoma, colorectal melanoma, or a colorectal neuroendocrine tumor. In a particular embodiment, the cancer is metastatic colon cancer. In another embodiment, the cancer is a stomach cancer (e.g., gastric adenocarcinoma, lymphoma, or sarcoma), or metastasis thereof In another embodiment, the cancer is an esophageal cancer (e.g., a squamous cell carcinoma or adenocarcinoma of the esophagus). [0356] The method can be useful in treating a relevant disorder at any stage or subclassification. For example, method can be used to treat early or late stage colon cancer, or colon cancer of any of stages 0, 1, IIA, RB, IIIA, IIIB, IIIC, and IV. [0357] In some embodiments, the method for treating cancer (e.g., colorectal, esophageal, or stomach cancer) comprises administering to a patient in need of such treatment a naked anti-GCC antibody molecule described herein. In other embodiments, the method comprises administering an immunoconjugate comprising an anti-GCC antibody molecule described herein and a cytotoxic agent. In some such embodiments, the irnmunoconjugate is characterized by formula (1), as described herein. In certain embodiments, the immunoconjugate is characterized by formula (I-1), (1-2), (1-3), (1-4), (1-5), (1-6), or (1-7) as described herein. In particular embodiments, the immunoconjugate is characterized by formula (1), (I-1), (1-2), (1-3), (1-4), (1-5), (1-6), or (1-7), wherein the variable Ab is an antibody molecule with features summarized in Tables I to 6. In certain embodiments, the variable Ab is a 5F9 antibody molecule or an Abx-229 antibody molecule. In certain particular embodiments, the immunoconjugate is characterized by formula (1-5) or (1-7), wherein the variable Ab is a 5F9 antibody molecule. [0358] Methods of administering antibody molecules and immunoconjugates are described above. Suitable dosages of the molecules used will depend on the age and weight of the subject and the particular compound used. 115 WO 2011/050242 PCT/US2010/053686 [0359] In some embodiments, the anti-GCC antibody molecule or immunoconjugate is administered in treatment cycles. A "treatment cycle" consists of a treatment period, during which the anti-GCC antibody molecule or immunoconjugate is administered as described above, followed by a rest period, during which no anti-GCC antibody molecule or immunoconjugate is administered. The treatment cycle can be repeated as necessary to achieve the desired effect. [0360] The anti-GCC antibodies described herein (e.g., naked anti-GCC antibody molecules or immunoconjugates comprising an anti-GCC antibody molecule and a therapeutic agent) may be used in combination with other therapies. For example, the combination therapy can include a composition of the present invention co-formulated with, and/or co-administered with, one or more additional therapeutic agents, e.g., one or more anti-cancer agents, e.g., cytotoxic or cytostatic agents, hormone treatment, vaccines, and/or other immunotherapies. In other embodiments, the anti-GCC antibodies are administered in combination with other therapeutic treatment modalities, including surgery, radiation, cryosurgery, and/or thermotherapy. Such combination therapies may advantageously utilize lower dosages of the administered therapeutic agents, thus avoiding possible toxicities or complications associated with the various monotherapies. [0361] Administered "in combination," as used herein, means that two (or more) different treatments are delivered to the subject during the course of the subject's affliction with the disorder, e.g., the two or more treatments are delivered after the subject has been diagnosed with the disorder and before the disorder has been cured or eliminated. In some embodiments, the delivery of one treatment is still occurring when the delivery of the second begins, so that there is overlap. This is sometimes referred to herein as "simultaneous" or "concurrent delivery." In other embodiments, the delivery of one treatment ends before the delivery of the other treatment begins. In some embodiments of either case, the treatment is more effective because of combined administration. For example, the second treatment is more effective, e.g., an equivalent effect is seen with less of the second treatment, or the second treatment reduces symptoms to a greater extent, than would be seen if the second treatment were administered in the absence of the first treatment, or the analogous situation is seen with the first treatment. In some embodiments, delivery is such that the reduction in a symptom, or other parameter related to the disorder is greater than what would be observed with one treatment delivered in the absence of the other. The effect of the two treatments can be partially additive, wholly 116 WO 2011/050242 PCT/US2010/053686 additive, or greater than additive. The delivery can be such that an effect of the first treatment delivered is still detectable when the second is delivered. [0362] In some embodiments, the anti-GCC antibody molecule or immunoconjugate thereof is used in combination with a chemotherapeutic agent. Non limiting examples of DNA damaging chemotherapeutic agents include topoisomerase I inhibitors (e.g., irinotecan, topotecan, camptothecin and analogs or metabolites thereof, and doxorubicin); topoisomerase II inhibitors (e.g., etoposide, teniposide, and daunorubicin); alkylating agents (e.g., melphalan, chlorambucil, busulfan, thiotepa, ifosfamide, carmustine, lomustine, semustine, streptozocin, decarbazine, methotrexate, mitomycin C, and cyclophosphamide); DNA intercalators (e.g., cisplatin, oxaliplatin, and carboplatin); DNA intercalators and free radical generators such as bleomycin; and nucleoside mimetics (e.g., 5-fluorouracil, capecitibine, gemcitabine, fludarabine, cytarabine, mercaptopurine, thioguanine, pentostatin, and hydroxyurea). [03631 Chemotherapeutic agents that disrupt cell replication include: paclitaxel, docetaxel, and related analogs; vincristine, vinblastin, and related analogs; thalidomide, lenalidomide, and related analogs (e.g., CC-5013 and CC-4047); protein tyrosine kinase inhibitors (e.g., imatinib mesylate and gefitinib); proteasome inhibitors (e.g., bortezomib); NF-B inhibitors, including inhibitors of JKB kinase; antibodies which bind to proteins overexpressed in cancers and thereby downregulate cell replication (e.g., trastuzumab, rituximab, cetuximab, and bevacizumab); and other inhibitors of proteins or enzymes known to be upregulated, over-expressed or activated in cancers, the inhibition of which downregulates cell replication. [03641 The selection of therapeutic agent(s) or treatment modality to be combined with an anti-GCC antibody molecule or immunoconjugate of the invention will depend on the disorder to be treated. The additional agent(s) or treatment modality may include, for example, standard approved therapies for the indication being treated. For example, when the anti-GCC antibody molecule or immunoconjugate thereof is used to treat colon cancer, it may be used in combination with, e.g., surgery; radiation therapy; 5-fluorouricil (5-FU), capecitibine, leucovorin, irinotecan, oxaliplatin, bevacizumab, cetuximab, panitumum, or combinations thereof (e.g., oxaliplatin/capecitibine (XELOX), 5-fluorouricil/leucovorin/ oxaliplatin (FOLFOX), 5 -fluorouricil/leucovorin/irinotecan (FOLFIRI), FOLFOX plus bevacizumab, or FOLFIRI plus bevacizumab). 117 WO 2011/050242 PCT/US2010/053686 [0365] In another aspect, the invention features the use of an anti-GCC antibody molecule or immunoconjugate as described herein in the manufacture of a medicament. In an embodiment, the medicament is for treating cancer, e.g., a gastrointestinal cancer. In some embodiments, the medicament comprises an anti-GCC antibody molecule having features surmmarized in Tables 1-6. In some embodiments, the medicament comprises a 5F9 antibody molecule or Abx-229 antibody molecule. [03661 Anti-GCC antibodies and immunoconjugates described herein can be used to detect the presence of GCC, e.g., to detect the presence of GCC in a biological sample, or to detect the presence or distribution of GCC in a subject. The term "detecting" as used herein encompasses quantitative or qualitative detection. Detecting GCC or GCC protein, as used herein, means detecting intact GCC protein or detecting a portion of the GCC protein that comprises the epitope to which the anti-GCC antibody molecule binds. [0367] Accordingly, in another aspect, the invention features, a method of detecting GCC protein, e.g., detecting a GCC expressing cell or tissue, e.g., a tumor cell, or a tumor having cells, that express GCC. The method comprises: contacting a material, e.g., a cell or tissue, e.g., a sample of a tumor which expresses GCC, with an anti-GCC antibody molecule, e.g., an anti-GCC antibody molecule described herein, under conditions which allow formation of a complex between the anti-GCC antibody molecule and GCC protein; and detecting formation of a complex between antibody molecule and GCC protein, to thereby detect the presence of GCC protein, e.g., to detect a GCC expressing cell or tumor. [0368J In an embodiment the anti-GCC antibody molecule is an immunoconjugate comprising a detectable label. [03691 In certain embodiments, the tissues include normal and/or cancerous tissues that express GCC at higher levels relative to other tissues, for example other tissue such as B cells and/or B cell associated tissues. [0370] Methods of detection described herein, whether in vitro or in vivo, can be used to evaluate a subject. In an embodiment the method is performed in vivo, and can be used, e.g., for imaging, staging, evaluation or diagnosis of a patient. In certain embodiments, the disorder is a cell proliferative disorder, such as a cancer or a tumor, e.g., colon cancer. 118 WO 2011/050242 PCT/US2010/053686 [0371] Thus, in another aspect, the invention provides, a method for detecting the presence of GCC protein in vitro (e.g., in a biological sample, such as a tissue biopsy, e.g., from a tumor tissue, from a subject) or in vivo (e.g., by in vivo imaging in a subject). The method comprises: (i) contacting a sample with an anti-GCC antibody molecule or immunoconjugate thereof, or administering to a subject, an anti-GCC antibody molecule or immunoconjugate thereof; and (ii) detecting formation of a complex between the anti-GCC antibody molecule and GCC protein. Complex formation is indicative of the presence or level of GCC. [0372] In embodiments the level of complex detected in the sample or subject is compared with a reference value, e.g., a value for complex formation or level of GCC. In an embodiment a level of GCC which exceeds a reference value is indicative of a GCC mediated disorder. [03731 In an embodiment the method comprises contacting a reference sample, e.g., a control sample (e.g., a control biological sample, such as plasma, tissue, biopsy) or a control subject) with an anti-GCC antibody molecule or immunoconjugate thereof and comparing the level of complex detected therein with the level detected in the sample or subject. [0374] In certain embodiments, a test cell or tissue is obtained from an individual suspected of having a disorder associated with increased expression of GCC. [03751 In an embodiment the level of GCC, in a sample from the subject, or in the subject, is compared with a reference level, e.g., the level of GCC in a control material, e.g., a normal cell of the same tissue origin as the subject's cell or a cell having GCC at levels comparable to such a normal cell. The method can comprise, e.g., responsive to the detected level of GCC, providing a diagnosis, a prognosis, an evaluation of the efficacy of treatment, or the staging of a disorder. A higher level of GCC in the sample or subject, as compared to the control material, indicates the presence of a disorder associated with increased expression of GCC. A higher level of GCC in the sample or subject, as compared to the control material, can also indicate, the relative lack of efficacy of a treatment, a relatively poorer prognosis, or a later stage of disease. The level of GCC can also be used to evaluate or select future treatment, e.g., the need for more or less aggressive treatment, or the need to switch from one treatment regimen to another. 119 WO 20111050242 PCT/US2010053686 [0376] The level of GCC can also be used to select or evaluate patients. E.g,, in embodiments patients whose tumor cells express high amounts of GCC on their surfaces would be considered good candidates for treatment with toxin-conjugated anti-GCC antibody molecules. In embodiments patients whose tumor cells express low amounts of GCC on their surfaces would not be as good candidates for this or might be candidates for combining the anti-GCC antibody molecule with an additional treatment method, or be candidates for naked antibody therapy. In another example, the dose of the toxin conjugated anti-GCC antibody molecule could be adjusted to reflect the number of GCC molecules expressed on the surfaces of tumor cells. Patients with high numbers of GCC molecules on their tumor cell surfaces might be treated with lower doses than patients with low numbers of GCC molecules. Detecting the presence of GCC-expressing tumor cells in vivo can allow identification of tissues into the primary GCC-expressing tumor has metastasized. Knowledge of which tissues have metastases can lead to targeted application of tumor therapy. [0377] As discussed above, the antibody molecules described herein permit assessment of the presence of a GCC protein in normal versus neoplastic tissues, through which the presence or severity of disease, disease progress and/or the efficacy of therapy can be assessed. For example, therapy can be monitored and efficacy assessed. In one example, a GCC protein can be detected and/or measured in a first sample obtained from a subject having an inflammatory disease and therapy can be initiated. Later, a second sample can be obtained from the subject and GCC protein in the sample can be detected and/or measured. A decrease in the quantity of GCC protein detected or measured in the second sample can be indicative of therapeutic efficacy. [0378] Exemplary cell proliferative disorders that may be evaluated, e.g., diagnosed, using an antibody disclosed herein include a proliferative disorder including, but not limited to, colon cancer, stomach cancer, esophageal cancer. [0379] In certain embodiments, a method, such as those described above, comprises detecting binding of an anti-GCC antibody to GCC expressed on the surface of a cell or in a membrane preparation obtained from a cell expressing GCC on its surface. In certain embodiments, the method comprises contacting a cell with an anti-GCC antibody under conditions permissive for binding of the anti-GCC antibody to GCC, and detecting whether a complex is formed between the anti-GCC antibody and GCC on the cell surface. 120 WO 2011/050242 PCT/US2010/053686 An exemplary assay for detecting binding of an anti-GCC antibody to GCC expressed on the surface of a cell is a "FACS" assay. [0380] Exemplary samples for methods described herein comprise tissue or body fluid, such as an inflammatory exudate, blood, serum, bowel fluid, stool sample, or biopsy. In one example, a sample (e.g., tissue and/or body fluid) can be obtained from an individual and a suitable immunological method can be used to detect and/or measure GCC protein expression. Suitable immunological methods for detecting or measuring GCC protein expression include enzyme-linked immunosorbent assays (ELISA), radioimmunoassay, immunohistology, flow cytometry, and the like. [0381] Anti-GCC antibody molecules used in methods described herein, e.g., in the in vivo and in vitro detection, e.g., diagnostic, staging, or imaging methods, can be directly or indirectly labeled with a detectable substance to facilitate detection of the bound or unbound binding agent. Suitable detectable substances include various biologically active enzymes, ligands, prosthetic groups, fluorescent materials, luminescent materials, chemiluminescent materials, bioluminescent materials, chromophoric materials, electron dense materials, paramagnetic (e.g., nuclear magnetic resonance active) materials, and radioactive materials. In some embodiments, the anti-GCC antibody molecule is coupled to a radioactive ion, e.g., indium (."In), iodine (..I or "'I), yttrium ("Y), lutetium (m7Lu), actinium ( 25 Ac), bismuth (mBi or " 2 Bi), sulfur (S), carbon (' 4 C), tritium (H), rhodium ('..Rh), technetium ( 9 mTc), praseodymium, or phosphorous ( 3 P); or a positron-emitting radionuclide, e.g., carbon-lI ("C), potassium-40 ("K), nitrogen-13 (3N), oxygen-15 ("O), fluorine-18 ("F), and iodine-121 (1211). [0382] Exemplary labels include fluorophores such as rare earth chelates or fluorescein and its derivatives, rhodamine and its derivatives, dansyl, umbelliferone, luceriferases, e.g., firefly luciferase and bacterial luciferase (U.S. Pat. No. 4,737,456), luciferin, and 2,3-dihydrophthalazinediones Other exemplary labels include horseradish peroxidase (HRP), alkaline phosphatase, galactosidase, glucoamylase, lysozyme, saccharide oxidases, e.g., glucose oxidase, galactose oxidase, and glucose 6-phosphate dehydrogenase, heterocyclic oxidases such as uricase and xanthine oxidase, coupled with an enzyme that employs hydmgen peroxide to oxidize a dye precursor such as HRP, lactoperoxidase, or microperoxidase, biotin/avidin, spin labels, bacteriophage labels, stable free radicals, and the like. 121 WO 2011/050242 PCT/US2010/053686 [0383] Fluorophore and chromophore labeled antibody molecules can be prepared from standard moieties known in the art. Since antibodies and other proteins absorb light having wavelengths up to about 310 nm, the fluorescent moieties should be selected to have substantial absorption at wavelengths above 310 nm and preferably above 400 un. A variety of suitable fluorescent compounds and chromophores are described by Stryer Science, 162:526 (1968) and Brand, L. et al. Annual Review ofBiochemistry, 41:843-868 (1972). The antibodies can be labeled with fluorescent chromophore groups by conventional procedures such as those disclosed in U.S. Patent Nos. 3,940,475, 4,289,747, and 4,376,110. [03841 One group of fluorescers having a number of the desirable properties described above is the xanthene dyes, which include the fluoresceins derived from 3,6-dihydroxy-9-henylxanthhydrol and resamines and rhodamines derived from 3,6-diamino-9-phenylxanthydrol and lissanime rhodamine B. The rhodamine and fluorescein derivatives of 9-o-carboxyphenylxanthhydrol have a 9-o-carboxyphenyl group. Fluorescein compounds having reactive coupling groups such as amino and isothiocyanate groups such as fluorescein isothiocyanate and fluorescamine are readily available. Another group of fluorescent compounds are the naphthylamines, having an amino group in the a or p position. [0385] Labeled antibody molecules can be used, for example, diagnostically and/or experimentally in a number of contexts, including (i) to isolate a predetermined antigen by standard techniques, such as affinity chromatography or immunoprecipitation; (ii) to detect a predetermined antigen (e.g., in a cellular lysate or cell supernatant) in order to evaluate the abundance and pattern of expression of the protein; (iii) to monitor protein levels in tissue as part of a clinical testing procedure, e.g., to determine the efficacy of a given treatment regimen [03861 Certain other methods can be used to detect binding of anti-GCC antibodies to GCC. Such methods include, but are not limited to, antigen-binding assays that are known in the art, such as western blots, radioimmunoassays, ELISA (enzyme linked immunosorbent assay), "sandwich" Immunoassays, immunoprecipitation assays, fluorescent inmunoassays, protein A immunoassays, and immunohistochemistry (IHC). 122 WO 2011/050242 PCT/US2010/053686 [0387] Complex formation between the anti-GCC antibody molecule and GCC can be detected by measuring or visualizing either the antibody (or antibody fragment) bound to the GCC antigen or unbound antibody molecule. Conventional detection assays can be used, e.g., western blots, radioimmunoassays, ELISA (enzyme linked immunosorbent assay), "sandwich" immunoassays, immunoprecipitation assays, fluorescent immunoassays, protein A immunoassays, and immunohistochemistry (IHC) or radioimmunoassay (RIA). [03881 Alternative to labeling the anti-GCC antibody molecule, the presence of GCC can be assayed in a sample by a competition immunoassay utilizing standards labeled with a detectable substance and an unlabeled anti-GCC antibody molecule. In this assay, the biological sample, the labeled standards and the GCC binding agent are combined and the amount of labeled standard bound to the unlabeled antibody is determined. The amount of GCC in the sample is inversely proportional to the amount of labeled standard bound to the GCC binding agent. [0389] It is also possible to directly detect GCC to anti-GCC antibody molecule complex formation without further manipulation or labeling of either component (GCC or antibody molecule), for example by utilizing the technique of fluorescence energy transfer (FET, see, for example, Lakowicz et al, U.S. Patent No. 5,631,169; Stavrianopoulos, et A., U.S. Patent No. 4,868,103). A fluorophore label on the first, 'donor' molecule is selected such that, upon excitation with incident light of appropriate wavelength, its emitted fluorescent energy will be absorbed by a fluorescent label on a second 'acceptor' molecule, which in turn is able to fluoresce due to the absorbed energy. Alternately, the 'donor' protein molecule may simply utilize the natural fluorescent energy of tryptophan residues. Labels are chosen that emit different wavelengths of light, such that the 'acceptor' molecule label may be differentiated from that of the 'donor'. Since the efficiency of energy transfer between the labels is related to the distance separating the molecules, spatial relationships between the molecules can be assessed. In a situation in which binding occurs between the molecules, the fluorescent emission of the 'acceptor' molecule label in the assay should be maximal. An FET binding event can be conveniently measured through standard fluorometric detection means well known in the art ( e.g., using a fluorimeter). 123 WO 2011/050242 PCT/US2010/053686 [0390] In another example, determination of the ability of an antibody molecule to recognize GCC can be accomplished without labeling either assay component (GCC or antibody molecule) by utilizing a technology such as real-time Biomolecular Interaction Analysis (BIA) (see, e.g., Sjolander, S. and Urbaniczky, C., 1991, Anal. Chem. 63:2338 2345 and Szabo et aL, 1995, Curr. Opin. Struct. BioL 5:699-705). As used herein, "BIA" or "surface plasmon resonance" is a technology for studying biospecific interactions in real time, without labeling any of the interactants (e.g., BIACORETM). Changes in the mass at the binding surface (indicative of a binding event) result in alterations of the refractive index of light near the surface (the optical phenomenon of surface plasmon resonance (SPR)), resulting in a detectable signal which can be used as an indication of real-time reactions between biological molecules. [0391] In still another embodiment, the invention provides a method for detecting the presence of GCC-expressing tumor tissues in vivo. The method includes (i) administering to a subject (e.g., a patient having a cancer) an anti-GCC antibody or antigen binding fragment thereof, preferably a antibody or antigen binding fragment thereof conjugated to a detectable label or marker; (ii) exposing the subject to a means for detecting said detectable label or marker to the GCC-expressing tissues or cells. [0392] Examples of labels useful for diagnostic imaging in accordance with the present invention are radiolabels such as 311, "'In, 68 Ga, 1I, 99 "Tc, 32 P, 1251, 3H, ' 4 C, and 88 Rh, fluorescent labels such as fluorescein and rhodamine, nuclear magnetic resonance active labels, positron emitting isotopes detectable by a single photon emission computed tomography ("SPECT") detector or positron emission tomography ("PET") scanner, chemiluminescers such as luciferin, and enzymatic markers such as peroxidase or phosphatase. Short-range radiation emitters, such as isotopes detectable by short-range detector probes, such as a transrectal probe, can also be employed. The antibody can be labeled with such reagents using techniques known in the art. For example, see Wensel and Meares (1983) Radioimmunoimaging and Radioimmunotherapy, Elsevier, New York, for techniques relating to the radiolabeling of antibodies. See also, D. Colcher et at. Meth. Enzymol. 121: 802-816 (1986. [03931 In the case of a radiolabeled antibody, the antibody is administered to the patient, is localized to the tumor bearing the antigen with which the antibody reacts, and is detected or "imaged" in vivo using known techniques such as radionuclear scanning using 124 WO 2011/050242 PCT/US2010/053686 e.g., a gamma camera or emission tomography or computed tomography. See e.g., A.R. Bradwell et al., "Developments in Antibody Imaging", Monoclonal.Antibodiesfor Cancer Detection and Therapy, R.W. Baldwin et al., (eds.), pp 65-85 (Academic Press 1985). Alternatively, a positron emission transaxial tomography scanner, such as designated Pet VI located at Brookhaven National Laboratory, can be used where the radiolabel emits positrons (e.g., "C, 'F, "O, and 'N). [03941 In other embodiments, the invention provides methods for determining the dose, e.g., radiation dose, that different tissues are exposed to when a subject, e.g., a human subject, is administered an anti-GCC antibody molecule that is conjugated to a radioactive isotope. The method includes: (i) administering an anti-GCC antibody molecule as described herein, e.g., a anti-GCC antibody molecule, that is labeled with a radioactive isotope to a subject; (ii) measuring the amount of radioactive isotope located in different tissues, e.g., tumor, or blood, at various time points until some or all of the radioactive isotope has been eliminated from the body of the subject; and (iii) calculating the total dose of radiation received by each tissue analyzed. The measurements can be taken at scheduled time points, e.g., day 1, 2, 3, 5, 7, and 12, following administration (at day 0) of the radioactively labeled anti-GCC antibody molecule to the subject. The concentration of radioisotope present in a given tissue, integrated over time, and multiplied by the specific activity of the radioisotope can be used to calculate the dose that a given tissue receives. Pharmacological information generated using anti-GCC antibody molecules labeled with one radioactive isotope, e.g., a gamma-emitter, e.g., i'In, can be used to calculate the expected dose that the same tissue would receive from a different radioactive isotope which cannot be easily measured, e.g., a beta-emitter, eg., 90 Y. Anti-GCC Antibody Sequences [0395] Anti-GCC antibodies were generated by several methods, as is discussed in more detail in the Examples. Briefly, mouse monoclonal antibodies 3G1 8F1 and 10B8 were generated by traditional immunization technology in conventional mice. Human monoclonal antibodies 1D2, 5F9, 5H3, 6H8, 8C2, and 1OC10 were generated using transgenic mice that generate filly human IgG2 antibodies, utilizing Abgenix XENOMOUSE transgenic technology, and isolated using hybridoma technology. Human mAb Abx-012, mAb Abx-020, mAb Abx-106, mAb Abx-198, mAb Abx-221, mAb Abx 229, mAb Abx-338 and mAb Abx-393 were generated using transgenic mice that generate 125 WO 2011/050242 PCT/US2010/053686 fully human IgG2 antibodies. Single antibodies were isolated using Abgenix SLAM technology. These were used to make fully human IgGI antibodies. Specificity of the antibodies against GCC was tested by ELISA and flow cytometry (FCM). A subset of the generated antibodies was selected for further characterization. [0396] Table 1 below summarizes fbr several anti-GCC antibodies the antibody designation, the immunogen used to generate the antibody, the animal used, the source, the species and the isotype isolates. Table 1 Ab Immunogen Animal Source Species Isotvoe 3G1 TOKI 07-hIg C57 conventional mouse Hybridoma Mouse IgG1,k 8F1 TOK107-hIg C57 conventional mouse Hybridoma Mouse IgG1,k 101B8 TOK107-hIg C57 conventional mouse Hybridoma Mouse IgG1 ,k 1D3 TOK107-hg C57 conventional mouse Hybridoma Mouse IgG1 ,k 8E12 TOK107-hlg C57 conventional mouse Hybridoma Mouse IgGI,k 5F9 TOK107-hIg XenoMouse Hybridoma Human IgG2,k 1D2 TOK107-hIg XenoMouse Hybridoma Human IgG2,k 5H3 CHO-GC-C#27 cells XenoMouse Hybridoma Human IgG2,k 6H8 CHO-GC-C#27 cells XenoMouse Hybridoma Human IgG2,k 8C2 CHO-GC-C#27 cells XenoMouse Hybridoma Human IgG2,k 10C1O CHO-GC-C#27 cells XenoMouse Hybridoma Human IgG2,k 10D3 CHO-GC-C#27 cells XenoMouse Hybridoma Human IgG2,k 1C9 CHO-GC-C#27 cells XenoMouse Hybridoma Human IgG2,k 229 TOK107-hIg XenoMouse SLAM Human IgG1 ,k 012 TOK107-lg XenoMouse SLAM Human IgG1,k 221 TOK107-hIg XenoMouse SLAM Human IgG1,k 020 TOK107-hIg XenoMouse SLAM Human IgG1,k 338 TOK107-hIg XenoMouse SLAM Human IgG1,k 126 WO 2011/050242 PCTJUS2010/053686 106 TOK107-hIg XenoMouse SLAM Human IgGl,k 198 ITOK107-hIg XenoMouse SLAM Human IgG,k 393 TOK107-hIg XenoMouse SLAM Human IgT,k [0397 The sequences of the light and heavy chain variable regions were detennined. Table 2 below is a summary of the SEQ ID NOs for the variable regions of several antibodies. The amino acid and nucleic acid sequences for the variable regions of each of the heavy and light chains for murine and human anti-GCC antibodies are shown in Tables 3 and 4, respectively. [0398] The amino acid and nucleic acid sequences for each of the CDRs of the heavy and light chains for anti-GCC antibodies are shown in Tables 5 and 6, respectively. [0399] For example, Table 3, row 9 is an illustration of the encoded amino acid sequence of the mature heavy chain variable region of mAb 5F9 (SEQ ID NO:18); and the nucleic acid sequence encoding the mature heavy chain variable region of mAb 5F9 (SEQ ID NO: 17) is depicted in Table 4, row 9. The encoded amino acid sequences of mAb 5F9 heavy chain CDR (CDR) 1 (SEQ ID NO:106), CDR2 (SEQ ID NO:108) and CDR3 (SEQ ID NO:1 10) are depicted in Table 5, rows 25-27, respectively; and the nucleic acid sequences of mAb 5F9 heavy chain CDRJ (SEQ ID NO:105), CDR2 (SEQ ID NO:107) and CDR3 (SEQ ID NO:109) are shown in Table 6, rows 25-27, respectively. [04001 Table 3, row 10 is an illustration of the encoded amino acid sequence of the mature light chain variable region of mAb 5F9 (SEQ ID NO:20); and the nucleic acid sequence encoding the mature kappa light chain variable region of mAb 5F9 (SEQ ID NO:19) is depicted in Table 4, row 10. The encoded amino acid sequences of mAb 5F9 light chain CDR (CDR) I (SEQ ID NO: 112), CDR2 (SEQ ID NO: 114) and CDR3 (SEQ ID NO: 116) are depicted in Table 5, rows 28-30, respectively; and the nucleic acid sequences of mAb 5F9 light chain CDR1 (SEQ ID NO:111), CDR2 (SEQ ID NO:113) and CDR3 (SEQ ID NO: 115) are shown in Table 6, rows 28-30, respectively. [0401] Sequencing of the CDRs allowed determination of the abundance of residues that might serve as toxin conjugation sites. An unpaired free cysteine in the antigen binding region could be a site for auristatin conjugation and a lysine could be a site for maytansine conjugation. Toxin conjugation to an amino acid of the CDR would raise 127 WO 2011/050242 PCT/US2010/053686 the concern of altering the binding affinity of the antibody to GCC. Thus, in embodiments the CDRs lack an amino acid which can be conjugated to a therapeutic agent. 128 WO 2011/050242 PCT/US2010/053686 Table 2. Summary of SEQ ID NOs for the variable regions of monoclonal antibodies. mAb IgG chain NA SEQ ID AA SEQ ID 3G1 Heavy chain 1 2 Light chain 3 4 8E12 Heavy chain 5 6 Light chain 7 8 SF1 Heavy chain 9 10 Light chain 11 12 1D3 Heavy chain 13 14 Light chain 15 16 5F9 Heavy chain 17 18 Light chain 19 20 5H3 Heavy chain 21 22 Light chain 23 24 6118 Heavy chain 25 26 Light chain 27 28 8C2 Heavy chain 29 30 Light chain 31 32 10C10 Heavy chain 33 34 Light chain 35 36 10D3 Heavy chain 286 287 Light chain 288 289 Abx-012 Heavy chain 238 239 129 WO 2011/050242 PCT/US2010/053686 Light chain 240 241 Abx-020 Heavy chain 37 38 Light chain 39 40 Abx-106 Heavy chain 242 243 Light chain 244 245 Abx-198 Heavy chain 41 42 Light chain 43 44 Abx-221 Heavy chain 246 247 Light chain 248 249 Abx-229 Heavy chain 45 46 Light chain 47 48 Abx-338 Heavy chain 49 50 Light chain 51 52 Abx-393 Heavy chain 53 54 Light chain 55 56 130 WO 2011/050242 PCTUS2010/053686 Table 3. Amino acid sequence of mAb variable region mAb IgG SEQ Amino Acid Sequence chain ID NO: I 3G1 Heavy 2 QVQLKESGPGLVAPSQSLSITCTVSGFSLSRNAISWVRQP chain PGKGLEWLGVIWTGGGTNYNSALKSRLSIRKENSKSQVF LKMNSLQTEDTARYFCARSGYDGFDYWGQGTLVTVSA 2 3G1 Light 4 QIVLTQSPAIMSASPGEKVTMTCSASSSVNYMHWYQQK chain SGTSPKRWIYDTSKLASGVPARFSGSGSGTSYSLTITSME AEDAATYYCQQWSGNPYTFGGGTKLEIK 3 8E12 Heavy 6 QVQLKQSGAELVKPGASVKISCKASGYTFTDYYINWVK chain QRPGQGLEWIGKIGPRSGNTYYNEKFKGKATLTADKSSS TAYMQLSSLTSEDSAVYFCARWDAYWGQGTLVTVS 4 8E12 Light 8 DVVMTQTPLSLSVTIGQPASISCKSSOSLLYSNGKTYLN chain WLQQRPGQAPKHLMYOVSKLDPGIPDRFSGSGSETDFTL KISRVEAEDLGVYYCLQGTYYPYTFGGGTKLEIK 5 8F1 Heavy 10 QVQLQQPGAELVKPGASVQMSCKASGYIFTGYWMYWV chain KQRPGQGLEWIGRIHPSDSNTNYNOKFKGKATLTVDKSS STAYMQLSSLTSEDSAVYYCTHALAYWGQGTILVTVS 6 SF1 Light 12 DVVLTQTPLTLSITIGQPASISCKSSOSLLYSNGKTYLSWL chain LQRPGQSPKRLIYLVSQLDSGVPDRFTGSGSGTDFTLKIS RVEAEDLGVYYCVOGTHLFTFGSGTKLEIK 7 1D3 Heavy 14 QVQLKQSGAELVKPGASVKMSCKASGYTFrDYYINWV chain KQRPGQGLEWIGKIGPRSGSTYYNEKFKGKATLTADKSS STAYMQLSSLTSEDSAVYFCARWDAYWGQGTLVTVSA 131 WO 2011/050242 PCT/US2010/053686 8 1D3 Light 16 DVVMTQTPLSLSVTIGQPASISCKSSQSLLYSNGKTYLN chain WLQQRPGQAPKHLMYQVSKLDPGIPDRFSGSGSETDFTL KISRVEAEDLGVYYCLQGTYYPYTFGGGTKLEIK 9 5F9 Heavy 18 QVQLQQWGAGLLKPSETLSLTCAVFGGSFSGYYWSWIR chain QPPGKGLEWIGEINHRGNTNDNPSLKSRVTISVDTSKNQF ALKLSSVTAADTAVYYCARERGYTYGNFDHWGQGTLV TVSS 10 5F9 Light 20 EIVMTQSPATLSVSPGERATLSCRASQSVSRNLAWYQQK chain PGQAPRLLIYGASTRATGIPARFSGSGSGTEFTLTIGSLQS EDFAVYYCQQYKTWPRTFGQGTNVEIK 11 5H3 Heavy 22 QVQLVESGGGLVKPGGSLRLSCAASGFTFSDCYMSWIR chain QSPGKGLEWVSYITTSGNTIYYADSVKGRFTISRDNAKN SLYLQMNSLRAEDTAVYYCARDWGWFYGMDVWGQGT TVTVSS 12 5H3 Light 24 DIVMTQTPLSLSVTPGQPASISCKSSQSLLHNDGKTYLY chain WYLQKPGQPPQLLIYEVSNRFSGVPDRFSSSGSXTDFTLK ISRVEAEDVGVYYCMQSIQLPRTFGQGTKVEIK 13 6H8 Heavy 26 QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYGMHWVR chain QAPGKGLEWVAAIWYDGSNKYYADSVKGRFTISRDNSK NTLYLQMNSLRAEDTAVYYCARGRSSSYFDYWGQGTL VTVSS 14 6H8 Light 28 DIVMTQTPLSSPVTLGQPASISCRSSQSLVHSDGNTYLSW chain LQQRPGQPPRLLIYKTSNRFSGVPDRFSGSGAGTDFTLKI SRVGAEDVGVYYCMQATQFPTFGQGTRLEIK 15 8C2 Heavy 30 QVQLVESGGGVVQPGRSLRLSCVASGFTFSSYGMHWVR chain QAPGKGLEWVGAIWYDGSNKYYAASVKGRFTISRDNSK NTLYLQMNSLRAEDTAVFYCARGRSSSYFDYWGQGTLV TVSS 132 WO 2011/050242 PCT/US2010/053686 16 8C2 Light 32 DIVMTQTPLSSPVTLGQPASISCRSSQSLVHSDGNTYLSW chain LQQRPGQPPRLLIYKTSNRFSGVPDRFSGSGAGTDFTLKI SRVGAEDVGVYYCMQATQFPTFGQGTRLEIK 17 1OC10 Heavy 34 QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYGMHWVR chain QAPGKGLEWVAAIWYDGSNKYYADSVKGRFTISRDNSK NTLYLQMNSLRAEDTAVYYCARGRSSSYFDYWGQGTL VTVSS 18 1OC1O Light 36 DIVMTQTPLSSPVTLGQPASFSCRSSQSLVHSDGNTYLS chain WLQQRPGQPPRLLIYKISNRFSGVPDRFSGSGAGTDFTLK ISRVEAEDVGVYYCMQATQFPTFGQGTRLEIK 35 10D3 Heavy 287 QVQLQQWGAGLLKPSETLSLTCAVFGGSFSGYYWSWIR chain QPPGKGLEWIGEINHRGNTNDNPSLKSR VTISVDTSKNQFALKLSSVTAADTAVYYCARERGYTYG NFDHWGQGTLVTVSS 36 10D3 Light 289 EIVLTQSPGTLSLSPGERATLSCRASQSVSSRYLAWYQQ chain KPGQAPRLLIYGASSRATGTPDRFSGSGSGTDFTLTISRLE PEDFAVYFCQQYERSFTFGPGTKVD 19 Abx- Heavy 239 QVQLQESGPGLVKPSETLSLTCTVSGASISHYYWSWIRQ 012 chain PAGKGLEWIGRIYISGRTSYNPSLKSRVTVSVDTSKNQFS LKLSSVTAADTAVYYCARDRLTGYFDYWGQGTLVTVSS 20 Abx- Light 241 EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQK 012 chain PGQAPRLLIYGASSRAAGIPDRFSGSGSGTDFTLTISRLEP EDFAVYYCQQYGSSLTFGGGTKVEIK 21 Abx- Heavy 38 QVQLVESGGGLVKPGGSLRLSCAASGFTFSDYYMSWIR 020 chain QAPGKGLEWISYITSSGSTIYYSASVKGRFTISRDNAKNS LYLQMNSLRAEDTAVYYCARDFSGWFGVHFDYWGQGT LVTVSS 133 WO 2011/050242 PCT/US2010/053686 22 Abx- Light 40 DIVMTQTPLSLSVTPGQPASISCKSSQSLLHSDGKTYLYW 020 chain YLQKPGQPPQLLIYEVSNRFSGVPNRFSGSGSGTDFTLKI SRVEAEDVGVYYCMQSIQLTWTFGQGTKVEIK 23 Abx- Heavy 243 QVQLQESGPGLVKPSETLSLTCTVSGASISHYYWSWIRQ 106 chain PAGKGLEWIGRIYISGRTSYNPSLKSRVTVSVDTSKNQFS LKLSSVTAADTAVYYCARDRLTGYFDYWGQGTLVTVSS 24 Abx- Light 245 EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQK 106 chain PGQAPRLLIYGTSSRATGIPDRFSGSGSGTDFTLTISRLEP EDFAVYYCQQYGSSPMCSFGQGTKLEIK 25 Abx- Heavy 42 EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVR 198 chain QAPGKGLDWVSDISGSGGSTYYADSVKGRFTISRDNSKN TLYLQMHSLSAEDTAIYYCAKRRWQGYFDLWGRGTLV TVSS 26 Abx- Light 44 EIVLTQSPGTLSLSPGERATLSCRARQRVDSRYLAWYQQ 198 chain KPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLE PEDFAVYYCQQYGSSPLTFGGGTKVEIK 27 Abx- Heavy 247 EVQLLESGGGLVQPGGSLRLSCAASGFTFSRYAMNWVR 221 chain QAPGKGLEWVSGISGSGGSTYYADSVKGRFTISRDNSKN TLYLQMNSLRAEDTAVYYCAKDRDFWSGPFDYWGQGT LVTVSS 28 Abx- Light 249 EIVMTPSSATLSVSPGERATLSCRASQSVSRSLAWYQQK 221 chain PGQAPRLLIYGASTRATGIPARFSGSGSGTEFTLTISSLQS EDVAVYYCQQYNNWMCSFGQGTKLEIK 29 Abx- Heavy 46 EVQLLESGGGLVQPGGSLRLSCAASGFTFSRYAMNWVR 229 chain QAPGKGLEWVSGISGSGGRTYYADSVKGRFTISRDNSKN TLYLQMNSLRAEDTAVYYCAKDRDFWSGPFDYWGQGT LVTVSS 30 Abx- Light 48 EIVMTPSSATLSVSPGERATLSCRASQSVSRNLAWYQQK 229 chain PGQAPRLLIYGASTRATGIPARFSGSGSGTEFTLTISSLQS EDFAVYYCHQYSNWMCSFGQGTKLEIK 134 WO 2011/050242 PCT/US2010/053686 31 Abx- Heavy 50 QVQLQESGPGLVKPSETLSLTCTVSGGSIRSYYWSWIRQP 338 chain AGKGLEWIGRIYISGRTTFNPSLKSRVTISVDTSKNQFSLK LSSVTAADTAVYFCARDRYYGYLDYWGQGTLVTVSS 32 Abx- Light 52 EIVLTQSPGTLSLSPGERATLSCRASQSVSRSYLAWYQQ 338 chain KPGQAPRLLIYDASSRATGIPDRFSGSGSGTDFTLTISRLE PEDFAVYYCQQYGSSPSTFGQGTRLEIK 33 Abx- Heavy 54 QVQLQESGPGLVKPSETLSLTCTVSGGSIRHYYWSWIRQ 393 chain PPGKGLEWIGYlYYSGSTNYNLSLKSRVTISRDTSKNQVS LKLSSVTAADTAVYYCAAGMGFDYWGQGTLVTVSS 34 Abx- Light 56 DIQMTQSPSSLSASIGDRVTITCRASQAIRNDLGWYQLKP 393 chain GKAPKRLIYSASSLQSGVPSRFSGSGSGTEFTLTISSLQPE DSATYYCLQHNSFPPTFGQGTKVE1K 135 WO 2011/050242 PCT/US2010/053686 Table 4. Nucleic acid sequence of mAb variable region mAb IgG SEQ Nucleic Acid Sequence chain ID NO: 1 3G1 Heavy I CAGGTGCAGCTGAAGGAGTCAGGACCTGGCCTGGTG chain GCGCCCTCACAGAGCCTGTCCATCACATGCACTGTC TCTGGGTTCTCATTAAGCAGAAATGCTATAAGCTGG GTTCGCCAGCCACCAGGAAAGGGTCTGGAGTGGCTT GGAGTAATATGGACTGGTGGAGGCACAAATTATAAT TCAGCTCTCAAATCCAGACTGAGCATCCGCAAAGAG AACTCCAAGAGTCAAGTTTTCTTAAAAATGAACAGT CTACAAACTGAAGACACAGCCAGGTACTTCTGTGCC AGAAGTGGTTACGACGGGTTTGATTACTGGGGCCAA GGGACTCTGGTCACTGTCTCTGCA 2 3G1 Light 3 CAGATTGTTCTCACCCAGTCTCCAGCAATCATGTCTG chain CATCTCCAGGGGAGAAGGTCACCATGACCTGCAGTG CCAGCTCAAGTGTAAATTACATGCACTGGTACCAGC AGAAGTCAGGCACCTCCCCCAAAAGATGGATTTATG ACACATCCAAACTGGCTTCTGGAGTCCCTGCTCGCTT CAGTGGCAGTGGGTCTGGGACCTCTrACTCTCTCAC AATCACCAGCATGGAGGCTGAAGATGCTGCCACTTA TTACTGCCAGCAGTGGAGTGGTAACCCGTACACGTT CGGAGGGGGGACCAAACTGGAAATAAAA 136 WO 2011/050242 PCT/US2010/053686 3 8E12 Heavy 5 CAGGTCCAGTTGAAGCAGTCTGGAGCTGAACTGGTG chain AAGCCTGGGGCTTCAGTGAAGATATCCTGCAAGGCT TCTGGCTACACCTTCACTGACTACTATATAAACTGGG TGAAGCAGAGGCCTGGACAGGGCCTTGAGTGGATTG GAAAGATTGGTCCTCGAAGTGGTAATACTrACTACA ATGAGAAGTTCAAGGGCAAGGCCACACrGACTGCA GACAAATCGTCCAGCACAGCCTACATGCAGCTCAGC AGCCTGACATCTGAGGACTCTGCAGTCTATTTCTGTG CAAGATGGGATGCTTACTGGGGCCAAGGGACTCTGG TCACTGTCTCT 4 8E12 Light 7 GATGTTGTGATGACCCAGACTCCACTGTCTTTGTCGG chain TTACCATTGGACAACCAGCCTCTATCTCTTGCAAGTC AAGTCAGAGCCTCTTATATAGTAATGGAAAGACATA TTTGAATTGGTTACAACAGAGGCCTGGCCAGGCTCC AAAGCACCTAATGTATCAGGTGTCCAAACTGGACCC TGGCATCCCTGACAGGTTCAGTGGCAGTGGATCAGA AACAGATTACACTTAAAATCAGCAGAGTGGAGGC TGAAGATTTGGGAGTTTATTACTGCTTGCAAGGTAC ATATTATCCGTACACGTTCGGAGGGGGGACCAAGCT GGAAATAAAG 5 8F1 Heavy 9 CAGGTCCAACTGCAGCAGCCTGGGGCTGAACTGGTG chain AAGCCTGGGGCTTCAGTGCAGATGTCCTGTAAGGCT TCTGGCTATATTTTCACCGGCTACrGGATGTACTGGG TGAAGCAGAGGCCTGGCCAAGGCCTTGAGTGGATTG GAAGGATTCATCCTTCTGATAGTAATACTAACTACA ATCAAAAGTTCAAGGGCAAGGCCACATTGACTGTAG ACAAATCCTCCAGCACAGCCTACATGCAACTCAGCA GCCTGACATCTGAGGACTCTGCGGTCTATTACTGTAC CCATGCCCTTGCTTACTGGGGCCAAGGGACTCTGGT CACTGTCTCT 137 WO 2011/050242 PCT/US2010/053686 6 8F1 Light 11 GATGTTGTGTTGACCCAGACTCCACTCACTTTGTCGA chain TTACCATTGGACAACCAGCCTCTATCTCTTGCAAGTC AAGTCAGAGCCTCTTATATAGTAATGGAAAAACCTA TTTGAGTTGGTTATTACAGAGGCCAGGCCAGTCTCC AAAGCGCCTAATCTATCTGGTGTCTCAACTGGACTCT GGAGTCCCTGACAGGTTCACTGGCAGTGGATCAGGA ACAGATTTTACACTGAAGATCAGCAGAGTGGAGGCT GAGGATTTGGGAGTGTATTACTGCGTGCAAGGTACA CATTTATTCACGTTCGGCTCGGGGACAAAGTTGGAA ATAAAA 7 1D3 Heavy 13 CAGGTCCAGCTGAAGCAGTCTGGAGCTGAGCTGGTG chain AAGCCTGGGGCTTCAGTGAAGATGTCCTGCAAGGCT TCTGGCTACACCTTCACAGACTACTATATAAACTGG GTGAAGCAGAGGCCTGGACAGGGCCTTGAGTGGATT GGAAAGATTGGTCCTAGAAGTGGTAGTACTTACTAC AATGAGAAGTCAAGGGCAAGGCCACACTGACTGC AGACAAATCCTCCAGCACAGCCTACATGCAGCTCAG CAGCCTGACATCTGAGGACTCTGCAGTCTATTTCTGT GCAAGATGGGATGCTTACTGGGGCCAAGGGACTCTG GTCACTGTCTCTGCA 8 1D3 Light 15 GATGTTGTGATGACCCAGACTCCACTGTCTTTGTCGG chain TTACCATTGGACAACCAGCCTCTATCTCTTGCAAGTC AAGTCAGAGCCTCTTATATAGTAATGGAAAGACATA TTTGAATTGGTTACAACAGAGGCCTGGCCAGGCTCC AAAGCACCTAATGTATCAGGTGTCCAAACTGGACCC TGGCATCCCTGACAGGTTCAGTGGCAGTGGATCAGA AACAGATTTTACACTTAAAATCAGCAGAGTGGAGGC TGAAGATTTGGGAGTTTATTACTGCTTGCAAGGTAC ATATTATCCGTACACGTTCGGAGGGGGGACCAAGCT GGAAATAAAA 138 WO 2011/050242 PCT/US2010/053686 9 5F9 Heavy 17 CAGGTGCAGCTACAGCAGTGGGGCGCAGGACTGTTG chain AAGCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCT TTGGTGGGTCCITCAGTGGTTACTACTGGAGCTGGAT CCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTG GGGAAATCAATCATCGTGGAAACACCAACGACAAC CCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGAC ACGTCCAAGAACCAGTTCGCCCTGAAGCTGAGTTCT GTGACCGCCGCGGACACGGCTGTTTATTACTGTGCG AGAGAACGTGGATACACCTATGGTAACTTTGACCAC TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA 10 5F9 Light 19 GAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCT chain GTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGG GCCAGTCAGAGTGTTAGCAGAAACTTAGCCTGGTAT CAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC TATGGTGCATCCACCAGGGCCACTGGAATCCCAGCC AGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT CTCACCATCGGCAGCCTGCAGTCTGAAGATTTTGCA GTTTATTACTGTCAGCAGTATAAAACCTGGCCTCGG . ACGTTCGGCCAAGGGACCAACGTGGAAATCAAA 11 5H3 Heavy 21 CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTC chain AAGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCC TCTGGATTCACCTTCAGTGACTGCTACATGAGCTGG ATCCGCCAGTCTCCAGGGAAGGGGCTGGAGTGGGTT TCATACATTACTACTAGTGGTAATACCATTTACTACG CAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGG ACAACGCCAAGAACTCACTGTATCTGCAAATGAACA GCCTGAGAGCCGAGGACACGGCCGTGTATTACTGTG CGAGAGACTGGGGATGGTTCTACGGTATGGACGTCT GGGGCCAAGGGACCACGGTCACCGTCTCCTCA 139 WO 2011/050242 PCT/US2010/053686 12 5H3 Light 23 GATATTGTGATGACCCAGACTCCACTCTCTCTGTCCG chain TCACCCCTGGACAGCCGGCCTCCATCTCCTGCAAGT CTAGTCAGAGCCTCCTGCATAATGATGGAAAGACCT ATTTGTATTGGTACCTGCAGAAGCCAGGCCAGCCTC CACAACTCCTGATCTATGAAGTTTCCAACCGGTTCTC TGGAGTGCCAGATAGGTTCAGTAGCAGCGGGTCNNG GACAGATTTCACACTGAAAATCAGCCGGGTGGAGGC TGAGGATGTTGGGGTTTATTACTGCATGCAAAGTAT ACAGCTTCCTCGGACGTTCGGCCAAGGGACCAAGGT GGAAATCAAA 13 6H8 Heavy 25 CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTC chain CAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCG -TCTGGATTCACCTTCAGTAGCTATGGCATGCACTGG GTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG GCAGCTATATGGTATGATGGAAGTAATAAATACTAT GCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGA GACAATTCCAAGAACACGCTGTATCTGCAAATGAAC AGCCTGAGACCCGAGGACACGGCTGTGTATTACTGT GCGAGAGGGAGGAGCAGCTCGTACTTTGACTATTGG GGCCAGGGAACCCTGGTCACCGTCTCCTCA 14 6H8 Light 27 GATATTGTGATGACCCAGACTCCACTCTCCTCACCTG chain TCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTC TAGTCAAAGCCTCGTACACAGTGATGGAAACACCTA CTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCC AAGACTCCTAATTTATAAGACTTCTAACCGCTTCTCT GGGGTCCCAGACAGATTCAGTGGCAGTGGGGCAGG GACAGATTTCACACTGAAAATCAGCAGGGTGGGAGC TGAGGATGTCGGGGTTTATTACTGCATGCAAGCTAC GCAATTTCCAACCTTCGGCCAAGGGACACGACTGGA GATTAAA 140 WO 2011/050242 PCT/US2010/053686 15 SC2 Heavy 29 caggtgcagctggtggagtctgggggaggcgtggtcagcotgggaggTCCCT chain GAGACTCTCCTGTGTAGCGTCTGGATTCACCTTCAGT AGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGC AAGGGGCTGGAGTGGGTGGGAGCTATATGGTATGAT GGAAGTAATAAATACTATGCAGCCTCCGTGAAGGGC CGATTCACCATCTCCAGAGACAATTCCAAGAACACG CTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGAC ACGGCTGTATTTTACTGTGCGAGAGGGAGGAGCAGC TCGTATTTTGACTACTGGGGCCAGGGAACCCTGGTC ACCGTCTCCTCA 16 8C2 Light 31 GATATTGTGATGACCCAGACTCCACTCTCCTCACCTG chain TCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTC TAGTCAAAGCCTCGTACACAGTGATGGAAACACCTA CTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCC AAGACTCCTAATrTATAAGACTTCTAACCGCTTCTCT GGGGTCCCAGACAGATTCAGTGGCAGTGGGGCAGG GACAGATTTCACACTGAAAATCAGCAGGGTGGGAGC TGAGGATGTCGGGGTTTATTACTGCATGCAAGCTAC GCAATTTCCAACCTTCGGCCAAGGGACACGACTGGA GATTAAA 17 1OC10 Heavy 33 CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTC chain CAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCG TCTGGATTCACCTTCAGTAGCTATGGCATGCACTGG GTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG GCAGCTATATGGTATGATGGAAGTAATAAATACTAT GCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGA GACAATTCCAAGAACACGCTGTATCTGCAAATGAAC AGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGT GCGAGAGGGAGGAGCAGCTCGTACTTTGACTATTGG GGCCAGGGAACCCTGGTCACCGTCTCCTCA 141 WO 20111050242 PCT/US2010/053686 18 loClo Light 35 GATATTGTGATGACCCAGACTCCACTCTCCTCACCTG chain TCACCCTTGGACAGCCGGCCTCCTTCTCCTGCAGGTC TAGTCAAAGCCTCGTACACAGTGATGGAAACACGTA CTTGAGTTGGCTTCAGCAGAGGCCAGGCCAGCCTCC AAGACTCCTAATTTATAAGATTTCTAACCGGTTCTCT GGGGTCCCAGACAGATTCAGTGGCAGTGGGGCAGG GACAGATTTCACACTGAAAATCAGCAGGGTGGAAGC TGAGGATGTCGGGGTTTATTACTGCATGCAAGCTAC ACAATTTCCAACCTTCGGCCAAGGGACACGACTGGA GATTAAA 35 10D3 Heavy 286 CAGGTGCA Chain GCTACAGCAGTGGGGCGCAGGACTGTTGAAGCCTTC GGAGACCCTGTCCC TCACCTGCGCTGTCTTTGGTGGGTCCTTCAGTGGTTA CTACTGGAGCTGG ATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATT GGGGAAATCAATCA TCGTGGAAACACCAACGACAACCCGTCCCTCAAGAG TCGAGTCACCATAT CAGTAGACACGTCCAAGAACCAGTTCGCCCTGAAGC TGAGTTCTGTGACC GCCGCGGACACGGCTGTTTATTACTGTGCGAGAGAA CGTGGATACACCTA TGGTAACTTTGACCACTGGGGCCAGGGAACCCTGGT CACCGTCTCCTCA 142 WO 2011/050242 PCT/US2010/053686 36 10D3 Light 288 GAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTT chain TGTCTCCAGGGGA AAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGT TAGCAGCAGGTACT TAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCA GGCTCCTCATCTAT GGTGCATCCAGCAGGGCCACTGGCACCCCAGACAGG TTCAGTGGCAGTGG GTCTGGGACAGACTTCACTCTCACCATCAGCAGACT GGAGCCTGAAGATT TTGCAGTGTATTTCTGTCAGCAGTATGAAAGGTCATT CACTTTCGGCCCT GGGACCAAAGTGGAT 19 Abx- Heavy 238 CAGGTGCAGTTGCAGGAGTCGGGCCCAGGACTGGTG 012 chain AAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCT CTGGTGC CTCCATCAGTCATTACTACTGGAGCTGGATCCGGCA GCCCGCCGGGAAGGGACTGGAATGGATTGGGCGTAT CTATATCA GTGGGAGGACCAGCTACAACCCCTCCCTCAAGAGTC GAGTCACCGTGTCAGTAGACACGTCCAAGAACCAGT TCTCCCTG AAGCTGAGCTCTGTGACCGCCGCGGACACGGCCGTG TATTACTGTGCGAGAGATCGGCTAACTGGGTACTTT GACTACTG GGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 143 WO 2011/050242 PCT/US2010/053686 20 Abx- Light 240 GAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTT 012 chain TGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGG CCAGTCA GAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCA GAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGG TGCATCCA GCAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCA GTGGGTCTGGGACAGACTTCACTCTCACCATCAGCA GACTGGAG CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATG GTAGCTCCCTCACTTrCGGCGGAGGGACCAAGGTGG AGATCAA AC 21 Abx- Heavy 37 CAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTC 020 chain AAGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCC TCTGGATTCACCTCAGTGACTACTACATGAGCTGG ATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGATT TCATACATTACTAGTAGTGGTAGTACCATATACTACT CAGCCTCTGTGAAGGGCCGATTCACCATCTCCAGGG ACAACGCCAAGAACTCACTGTATCTGCAAATGAACA GCCTGAGAGCCGAGGACACGGCCGTGTATTACTGTG CGAGAGATTTCAGTGGCTGGTTCGGAGTCCACTTTG ACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCT CG 144 WO 2011/950242 PCT/US2010/053686 22 Abx- Light 39 GATATTGTGATGACCCAGACTCCACTCTCTCTGTCCG 020 chain TCACCCCTGGACAGCCGGCCTCCATCTCCTGTAAGTC TAGTCAGAGCCTCCTGCATAGTGATGGAAAGACCTA TTTGTATrGGTACCTGCAGAAGCCAGGCCAGCCTCC ACAGCTCCTGATCTATGAAGTTTCCAACCGGTTCTCT GGAGTGCCAAATAGGTTCAGTGGCAGCGGGTCAGG GACAGATTTCACACTGAAAATCAGCCGGGTGGAGGC TGAGGATGTrGGGGTTTATrACTGCATGCAAAGTAT ACAACTTACGTGGACGTCGGCCAAGGGACCAAGGT GGAAATCAAA 23 Abx- Heavy 242 CAGGTGCAGTTGCAGGAGTCGGGCCCAGGACTGGTG 106 chain AAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCT CTGGTGC CTCCATCAGTCATTACTACTGGAGCTGGATCCGGCA GCCCGCCGGGAAGGGACTGGAATGGATTGGGCGTAT CTATATCA GTGGGAGGACCAGCTACAACCCCTCCCTCAAGAGTC GAGTCACCGTGTCAGTAGACACGTCCAAGAACCAGT TCTCCCTG AAGCTGAGCTCTGTGACCGCCGCGGACACGGCCGTG TATTACTGTGCGAGAGATCGGCTAACTGGGTACTTT GACTACTG GGGCCAGGGAACCCTGGTCACCGTCTCCTCAGC 145 WO 2011/050242 PCT/US2010/053686 24 Abx- Light 244 GAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTT 106 chain TGTCTCCAGGGGAAAGAGCCACCCTCTCCrGCAGGG CCAGTCA GAGTGTTAGCAGCAGCTAC'TAGCCTGGTACCAGCA GAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGG TACATCCA GCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCA GTGGGTCTGGGACAGACTTCACTCTCACCATCAGCA GACTGGAG CCTGAAGACTTTGCAGTGTATTACTGTCAGCAGTAT GGTAGCTCACCCATGTGCAGTTTTGGCCAGGGGACC AAGCTGGA GATCAAACG 25 Abx- Heavy 41 GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTA 198 chain CAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCC TCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGG GTCCGCCAGGCTCCAGGGAAGGGGCTGGACTGGGTC TCAGATATTAGTGGTAGTGGTGGTAGCACATACTAC GCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGA GACAATTCCAAGAACACGCTGTATCTGCAAATGCAC AGCCTGAGCGCCGAGGACACGGCCATATATTACTGT GCGAAACGGCGGTGGCAGGGGTACTTCGATCTCTGG GGCCGTGGCACCCTGGTCACTGTCTCCTCA 146 WO 2011/050242 PCTUS2010/053686 26 Abx- Light 43 GAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTT 198 chain TGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGG CCAGGCAGCGTGTTGACAGCAGGTACTTAGCCTGGT ACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCA TCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAG ACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA CTCTCACCATCAGCAGACTGGAGCCTGAAGATTITG CAGTGTATTACTGTCAGCAGTATGGTAGCTCACCGC TCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA 27 Abx- Heavy 246 GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTA 221 chain CAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCC TCTGGATT CACCTTTAGCCGCTATGCCATGAACTGGGTCCGCCA GGCTCCAGGGAAGGGGCTGGAGTGGGTCTCAGGTAT TAGTGGTA GTGGTGGTAGCACATACTACGCAGACTCCGTGAAGG GCCGGTTCACCATCTCCAGAGACAATTCCAAGAACA CGCTGTAT CTGCAAATGAACAGCCTGAGAGCCGAGGACACGGC CGTATATTACTGTGCGAAAGATCGCGATTTTTGGAG TGGTCCATT TGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC CTCAGC 147 WO 20111050242 PCT/[JS2010/053686 28 Abx- Light 248 GAAATAGTGATGACGCCGTCTTCAGCCACCCTGTCT 221 chain GTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGG GCCAGTCA GAGTGTTAGTAGAAGCTTAGCCTGGTACCAGCAGAA ACCTGGCCAGGCTCCCAGGCTCCTCATCTACGGTGC ATCCACCA GGGCCACTGGGATCCCAGCCAGGTTCAGTGGCAGTG GGTCTGGGACAGAATTCACTCTCACCATCAGCAGCC TGCAGTCT GAAGATGTTGCAGTTTATTACTGTCAGCAGTATAAT AACTGGATGTGCAGTTTTGGCCAGGGGACCAAGCTG GAGATCAA ACG 29 Abx- Heavy 45 GAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTA 229 chain CAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCC TCTGGATTCACCTTTAGCCGCTATGCCATGAACTGGG TCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCT CAGGTATTAGTGGGAGTGGTGGTAGGACATACTACG CAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAG ACAATTCCAAGAACACACTATATCTGCAAATGAACA GCCTGAGAGCCGAGGACACGGCCGTATATTACTGTG CGAAAGATCGCGATTTTTGGAGTGGTCCATTTGACT ACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA 148 WO 2011/050242 PCT/US2010/053686 30 Abx- Light 47 GAAATAGTGATGACGCCGTCTTCAGCCACCCTGTCT 229 chain GTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGG GCCAGTCAGAGTGTTAGTAGAAACTTAGCCTGGTAC CAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC TATGGTGCATCCACCAGGGCCACTGGTATCCCAGCC AGGTrCAGTGGCAGTGGGTCTGGGACAGAATTCACT CTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCA GTTTATTACTGTCACCAGTATAGTAACTGGATGTGCA GTTTTGGCCAGGGGACCAAGCTGGAGATCAAA 31 Abx- Heavy 49 CAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTG 338 chain AAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCT CTGGTGGCTCCATCAGAAGTTACTACTGGAGCTGGA TCCGGCAGCCCGCCGGGAAGGGACTGGAGTGGATTG GACGTATTTATATCAGTGGGAGGACCACCTTCAACC CCTCCCTCAAGAGTCGAGTCACCATATCAGTGGACA CGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTG TGACCGCCGCGGACACGGCCGTGTATTTCTGTGCGA GAGATAGATATTATGGCTACCTTGACTACTGGGGCC AGGGAACCCTGGTCACCGTCTCCTCA 32 Abx- Light 51 GAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTT 338 chain TGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGG CCAGTCAGAGTGTTAGCCGCAGTTACTTAGCCTGGT ACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCA TCTATGATGCATCCAGCAGGGCCACTGGCATCCCAG ACAGGTTCAGTGGCAGTGGGTCrGGGACAGACTTCA CTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTG CAGTGTATTACTGTCAGCAGTATGGTAGTTCACCGA GCACCTrCGGCCAAGGGACACGACTGGAGATTAAA 149 WO 2011/050242 PCTUS2010/053686 33 Abx- Heavy 53 CAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTG 393 chain AAGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCT CTGGCGGCTCCATCCGTCATTACTACTGGAGCTGGA TCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTG GGTATATCTATrACAGTGGGAGCACCAACTACAACC TCTCCCTCAAGAGTCGAGTCACCATATCAAGAGACA CGTCCAAGAATCAGGTCTCCCTG AAGCTGAGTTCTGTGACCGCTGCGGACACGGCCGTG TATTATTGTGCGGCGGGTATGGGCTTTGACTACTGG GGCCAGGGAACCCTGGTCACCGTCTCCTCA 34 Abx- Light 55 GACATCCAGATGACCCAGTCTCCTTCCTCCCTGTCTG 393 chain CATCTATAGGAGACAGAGTCACCATCACTTGCCGGG CAAGTCAGGCCATTAGAAATGATTTAGGCTGGTATC AGCTGAAACCGGGGAAAGCCCCTAAGCGCCTGATCT ATTCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAA GGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTC TCACAATCAGCAGCCTGCAGCCTGAGGATTCTGCAA CTTATTACTGTCTACAGCATAATAGTTTCCCTCCGAC GTTCGGCCAAGGGACCAAGGTGGAAATCAAA 150 WO 2011/050242 PCT/U1S2010/053686 Table 5: Amino acid sequence of CDRs mAb IgG SEQ ID Amino Acid Sequence chain NO: 1 3G1 VH 58 RNAIS CDR1 2 3G1 VH 60 VIWTGGGTNYNSALKS CDR2 3 3G1 VH 62 SGYDGFDY CDR3 4 3G1 VL 64 SASSSVNYMH CDR1 5 3G1 VL 66 DTSKLAS CDR2 6 3G1 VL 68 QQWSGNPYT CDR3 7 8E12 VH 70 DYYIN CDR1 8 8E12 VH 72 KIGPRSGNTYYNEKFKG CDR2 9 8E12 VII 74 WDAY CDR3 10 8E12 VI 76 KSSQSLLYSNGKTYLN CDRI1 151 WO 2011/050242 PCT/US2010/053686 11 8E12 VL 78 QVSKLDP CDR2 12 8E12 VL 80 LQGTYYPYT CDR3 13 8F1 VH 82 GYWMY CDR1 14 8F1 VH 84 RTIPSDSNTNYNQKFKG CDR2 15 8F1 VH 86 ALAY CDR3 16 8F1 VL 88 KSSQSLLYSNGKTYLS CDRI 17 8F1 VL 90 LVSQLDS CDR2 18 8F1 VL 92 VQGTHLFT CDR3 19 1D3 VH 94 DYYIN CDR1 20 1D3 VH 96 KIGPRSGSTYYNEKFKG CDR2 21 1D3 VH 98 WDAY CDR3 22 1D3 VL 100 KSSQSLLYSNGKTYL CDR1 152 WO 2011/050242 PCT/US2010/053686 23 1D3 VL 102 QVSKLDP CDR2 24 1D3 VL 104 LQGTYYPYT CDR3 25 5F9 VH 106 GYYWS CDR1 26 5F9 VH 108 EINHRGNTNDNPSLKS CDR2 27 5F9 VH 110 ERGYTYGNFDH CDR3 28 5F9 VL 112 RASQSVSRNLA CDR1 29 5F9 VL 114 GASTRAT CDR2 30 SF9 VL 116 QQYKTWPRT CDR3 31 5H3 VH 118 DCYMS CDR1 32 5H3 VH 120 YITTSGNTIYYADSVKG CDR2 33 5H3 VH 122 DWGWFYGMDV CDR3 34 5H3 VL 124 KSSQSLLHNDGKTYLY CDR1 153 WO 2011/050242 PCT/US2010/053686 35 5H3 VL 126 EVSNRFS CDR2 36 5H3 VL 128 MQSIQLPRT CDR3 37 6H8 VH 130 SYGMH CDR1 38 6H8 VH 132 AIWYDGSNKYYADSVKG CDR2 39 6H8 VH 134 GRSSSYFDY CDR3 40 6H8 VL 136 RSSQSLVHSDGNTYLS CDR1 41 6H8 VL 138 KTSNRFS CDR2 42 6H8 VL 140 MQATQFPT CDR3 43 8C2 VH 142 SYGMH CDR1 44 8C2 VII 144 AIWYDGSNKYYAASVKG CDR2 45 8C2 VH 146 GRSSSYFDY CDR3 46 8C2 VL 148 RSSQSLVHSDGNTYLS CDR1 154 WO 2011/050242 PCT/US2010/053686 47 8C2 VL 150 KTSNRFS CDR2 48 8C2 VL 152 MQATQFPT CDR3 49 10Ct1 VH 154 SYGMH CDR1 50 1OCt1 VH 156 AIWYDGSNKYYADSVKG CDR2 51 1OCt1 VH 158 GRSSSYFDY CDR3 52 1OC1 VL 160 RSSQSLVHSDGNTYLS CDRI 53 1OCt1 VL 162 KISNRFS CDR2 54 1OC1 VL 164 MQATQFPT CDR3 103 1OD3 VH 291 GYYWS CDR1 104 1OD3 VH 293 ElNHRGNTNDNPSLKS CDR2 105 lOD3 VH 295 ERGYTYGNFDH CDR3 106 IOD3 VL 297 RASQSVSSRYLA CDR1 155 WO 2011/050242 PCT/US2010/053686 107 10D3 VL 299 GASSRAT CDR2 108 10D3 VL 301 QQYERSFT CDR3 55 Abx-012 VH 251 HYYWS CDRI 56 Abx-012 VH 253 RIYISGRTSYNPSLKS CDR2 57 Abx-012 VH 255 DRLTGYFDY CDR3 58 Abx-012 VL 257 RASQSVSSSYLA CDR1 59 Abx-012 VL 259 GASSRAA CDR2 60 Abx-012 VL 261 QQYGSSLT CDR3 61 Abx-020 VH 166 DYYMS CDR1 62 Abx-020 VH 168 YITSSGSTIYYSASVKG CDR2 63 Abx-020 VH 170 DFSGWFGVHFDY CDR3 64 Abx-020 VL 172 KSSQSLLHSDGKTYLY CDR1 156 WO 2011/050242 PCT/US2010/053686 65 Abx-020 VL 174 EVSNRFS CDR2 66 Abx-020 VL 176 MQSIQLTWT CDR3 67 Abx-0106 VH 263 HYYWS CDR1 68 Abx-106 VH 265 RIYISGRTSYNPSLKS CDR2 69 Abx-106 VH 267 DRLTGYFDY CDR3 70 Abx-106 VL 269 RASQSVSSSYLA CDR1 71 Abx-106 VL 271 GTSSRAT CDR2 72 Abx-106 VL 273 QQYGSSPMCS CDR3 73 Abx-198 VH 178 SYAMS CDR1 74 Abx-198 VH 180 DISGSGGSTYYADSVKG CDR2 75 Abx-198 VH 182 RRWQGYFDL CDR3 76 Abx-198 VL 184 RARQRVDSRYLA CDR1I 157 WO 2011/050242 PCT/US210/053686 77 Abx-198 VL 186 GASSRAT CDR2 78 Abx-198 VL 188 QQYGSSPLT CDR3 79 Abx-221 VH 275 RYAMN CDRI 80 Abx-221 VH 277 GISGSGGSTYYADSVKG CDR2 81 Abx-221 VH 279 DRDFWSGPFDY CDR3 82 Abx-221 VL 281 RASQSVSRSLA CDRI 83 Abx-221 VL 283 GASTRAT CDR2 84 Abx-221 VL 285 QQYNNWMCS CDR3 85 Abx-229 VH 190 RYAMN CDR1 86 Abx-229 VH 192 GISGSGGRTYYADSVKG CDR2 87 Abx-229 VH 194 DRDFWSGPFDY CDR3 88 Abx-229 VL 196 RASQSVSRNLA CDR1 158 WO 2011/050242 PCT/JUS2010/053686 89 Abx-229 VL 198 GASTRAT CDR2 90 Abx-229 VL 200 HQYSNWMCS CDR3 91 Abx-338 VH 202 SYYWS CDRI 92 Abx-338 VH 204 RIYISGRTTFNPSLKS CDR2 93 Abx-338 VH 206 DRYYGYLDY CDR3 94 Abx-338 VL 208 RASQSVSRSYLA CDRI 95 Abx-338 VL 210 DASSRAT CDR2 96 Abx-338 VL 212 QQYGSSPST CDR3 97 Abx-393 VH 214 HYYWS CDR1 98 Abx-393 VH 216 YIYYSGSTNYNLSLKS CDR2 99 Abx-393 VH 218 GMGFDY CDR3 100 Abx-393 VL 220 RASQAIRNDLG CDRI 159 WO 2011/050242 PCT/US2010/053686 101 Abx-393 VL 222 SASSLQS CDR2 102 Abx-393 VL 224 LQHNSFPPT CDR3 109 consensus VH 302 x-x/Y-x/Y-M/W-S/N CDRI 110 consensus VH 303 x-I-x-x-SG-[x or none]-x-T/I-[y/T/S]-x-x-L/V-K-s/G CDR2 111 consensus VH 304 [4-6x]-G-[2-3x]-D-Y CDR3 112 consensus VL 305 R/K-A/S-SQS-V/L-S/L-[5-9x] CDR1 113 consensus VL 306 x-x-S-x-R-x-x CDR2 114 consensus VL 307 Q/H/M-Q-Y/S-[5-7x] CDR3 Table 6. Nucleic acid sequence of CDRs mAb IgG SEQ ID Nucleic Acid Sequence chain NO: 1 3G1 VH 57 AGAAATGCTATAAGC CDRI 160 WO 2011/050242 PCT/US2010/053686 2 3G1 VH 59 GTAATATGGACTGGTGGAGGCACAAATTAT CDR2 AATTCAGCTCTCAAATCC 3 3G1 VH 61 AGTGGTTACGACGGGTTTGATTAC CDR3 4 3G1 VL 63 AGTGCCAGCTCAAGTGTAAATTACATGCAC CDRI 5 3G1 VL 65 GACACATCCAAACTGGCTTCT CDR2 6 3G1 VL 67 CAGCAGTGGAGTGGTAACCCGTACACG CDR3 7 8E12 VH 69 GACTACTATATAAAC CDR1 8 8E12 VH 71 AAGATTGGTCCTCGAAGTGGTAATACTTACT CDR2 ACAATGAGAAGTTCAAGGGC 9 8E12 VH 73 TGGGATGCTTAC CDR3 10 8E12 VL 75 AAGTCAAGTCAGAGCCTCTTATATAGTAATG CDRI GAAAGACATATTTGAAT 11 8E12 VL 77 CAGGTGTCCAAACTGGACCCT CDR2 12 8E12 VL 79 TTGCAAGGTACATATTATCCGTACACG CDR3 13 SF1 VH 81 GGCTACTGGATGTAC CDRI 161 WO 2011/050242 PCTUS2010/053686 14 SF1 VH 83 AGGATICATCCTTCTGATAGTAATACTAACT CDR2 ACAATCAAAAGTTCAAGGGC 15 8F1 VH 85 GCCCTTGCTTAC CDR3 16 8F1 VL 87 AAGTCAAGTCAGAGCCTCTTATATAGTAATG CDR1 GAAAAACCTATTTGAGT 17 8F1 VL 89 CTGGTGTCTCAACTGGACTCT CDR2 18 8F1 VL 91 GTGCAAGGTACACATTTATTCACG CDR3 19 1D3 VH 93 GACTACTATATAAAC CDR1 20 1D3 VH 95 AAGATTGGTCCTAGAAGTGGTAGTACTTACT CDR2 ACAATGAGAAGTTCAAGGGC 21 1D3 VH 97 TGGGATGCTTAC CDR3 22 1D3 VL 99 AAGTCAAGTCAGAGCCTCTTATATAGTAATG CDRI GAAAGACATATTTGAAT 23 1D3 VL 101 CAGGTGTCCAAACTGGACCCT CDR2 24 1D3 VL 103 TTGCAAGGTACATATTATCCGTACACG CDR3 25 5F9 VH 105 GGTTACTACTGGAGC CDR1 162 WO 2011/050242 PCT/US2010/053686 26 5F9 VH 107 GAAATCAATCATCGTGGAAACACCAACGAC CDR2 AACCCGTCCCTCAAG 27 5F9 VH 109 GAACGTGGATACACCTATGGTAACTTTGACC CDR3 AC 28 5F9 VL 111 AGGGCCAGTCAGAGTGTTAGCAGAAACTTA CDRI GCC 29 5F9 VL 113 GGTGCATCCACCAGGGCCACT CDR2 30 5F9 VL 115 CAGCAGTATAAAACCTGGCCTCGGACG CDR3 31 5113 VH 117 GACTGCTACATGAGC CDRI 32 5H3 VH 119 TACATTACTACTAGTGGTAATACCATTTACT CDR2 ACGCAGACTCTGTGAAGGGC 33 5H3 VH 121 GACTGGGGATGGTTCTACGGTATGGACGTC CDR3 34 5H3 VL 123 AAGTCTAGTCAGAGCCTCCTGCATAATGATG CDR1 GAAAGACCTATTTG 35 5H3 VL 125 GAAGTTTCCAACCGGTTCTCT CDR2 36 5H3 VL 127 ATGCAAAGTATACAGCTTCCTCGGACG CDR3 37 6H8 VH 129 AGCTATGGCATGCAC CDR1 163 WO 2011/050242 PCTUS2010/053686 38 6HS VH 131 GCTATATGGTATGATGGAAGTAATAAATACT CDR2 ATGCAGACTCCGTGAAGGGC 39 6HS VH 133 GGGAGGAGCAGCTCGTACTTTGACTAT CDR3 40 6H8 VL 135 AGGTCTAGTCAAAGCCTCGTACACAGTGATG CDRI GAAACACCTACTTGAGT 41 6H8 VL 137 AAGACTTCTAACCGCTTCTCT CDR2 42 6H8 VL 139 ATGCAAGCTACGCAATTTCCAACC CDR3 43 8C2 VH 141 AGCTATGGCATGCAC CDR1 44 8C2 VH 143 GCTATATGGTATGATGGAAGTAATAAATACT CDR2 ATGCAGCCTCCGTGAAGGGC 45 8C2 VH 145 GGGAGGAGCAGCTCGTATTTTGACTAC CDR3 46 8C2 VL 147 AGGTCTAGTCAAAGCCTCGTACACAGTGATG CDRI GAAACACCTACTTGAGT 47 8C2 VL 149 AAGACTTCTAACCGCTTCTCT CDR2 48 8C2 VL 151 ATGCAAGCTACGCAATTTCCA CDR3 49 lOClO VII 153 AGCTATGGCATGCAC CDR1 164 WO 2011/050242 PCT/US2010/053686 50 10C1O VH 155 GCTATATGGTATGATGGAAGTAATAAATACT CDR2 ATGCAGACTCCGTGAAGGGC 51 1OC10 VH 157 GGGAGGAGCAGCTCGTACTTTGACTAT CDR3 52 10C10 VL 159 AGGTCTAGTCAAAGCCTCGTACACAGTGATG CDR1 GAAACACGTACTTGAGT 53 1OC10 VL 161 AAGATTTCTAACCGGTTCTCT CDR2 54 10C1 VL 163 ATGCAAGCTACACAATTTCCAACC CDR3 103 10D3 VH 290 GGTTACTACTGGAGC CDR1 104 10D3 VH 292 GAAATCAATCATCGTGGAAACACCAACGAC CDR2 AACCCGTCCCTCAAG 105 10D3 VH 294 GAACGTGGATACACCTATGGTAACTTTGACC CDR3 AC 106 10D3 VL 296 AGGGCCAGTCAGAGTGTTAGCAGCAGGTAC CDR1 TTAGCCT 107 10D3 VL 298 GGTGCATCCAGCAGGGCCACTG CDR2 108 10D3 VL 300 CAGCAGTATGAAAGGTCATTCACTT CDR3 55 Abx-012 VH 250 CATTACTACTGGAGC CDR1 56 Abx-012 VH 252 CGTATCTATATCAGTGGGAGGACCAGCTACA CDR2 ACCCCTCCCTCAAGAGT 165 WO 20111050242 PCT/US2010/053686 57 Abx-012 VH 254 GATCGGCTAACTGGGTACTTTGACTAC CDR3 58 Abx-012 VL 256 AGGGCCAGTCAGAGTGTTAGCAGCAGCTAC CDR1 TTAGCC 59 Abx-012 VL 258 GGTGCATCCAGCAGGGCCGCT CDR2 60 Abx-012 VIL 260 CAGCAGTATGGTAGCTCCCTCACT CDR3 61 Abx-020 VH 165 GACTACTACATGAGC CDR1 62 Abx-020 VH 167 TACATTACTAGTAGTGGTAGTACCATATACT CDR2 ACTCAGCCTCTGTGAAGGGC 63 Abx-020 VH 169 GATTTCAGTGGCTGGTTCGGAGTCCACTTTG CDR3 ACTAC 64 Abx-020 VL 171 AAGTCTAGTCAGAGCCTCCTGCATAGTGATG CDR1 GAAAGACCTATTTGTAT 65 Abx-020 VL 173 GAAGTTTCCAACCGGTTCTCT CDR2 66 Abx-020 VL 175 ATGCAAAGTATACAACTTACGTGGACG CDR3 67 Abx-106 VH 262 CATTACTACTGGAGC CDR1 68 Abx-106 VH 264 CGTATCTATATCAGTGGGAGGACCAGCTACA CDR2 ACCCCTCCCTCAAGAGT 166 WO 2011/050242 PCT/US2010/053686 69 Abx-106 VH 266 GATCGGCTAACTGGGTACTTTGACTAC CDR3 70 Abx-106 VL 268 AGGGCCAGTCAGAGTGTTAGCAGCAGCTAC CDR1 TTAGCC 71 Abx-106 VL 270 GGTACATCCAGCAGGGCCACT CDR2 72 Abx-106 VL 272 CAGCAGTATGGTAGCTCACCCATGTGCAGT CDR3 73 Abx-198 VH 177 AGCTATGCCATGAGC CDR1 74 Abx-198 VH 179 GATATTAGTGGTAGTGGTGGTAGCACATACT CDR2 ACGCAGACTCCGTGAAGGGC 75 Abx-198 VH 181 CGGCGGTGGCAGGGGTACTTCGATCTC CDR3 76 Abx-198 VL 183 AGGGCCAGGCAGCGTGTTGACAGCAGGTAC CDRI TTAGCC 77 Abx-198 VL 185 GGTGCATCCAGCAGGGCCACT CDR2 78 Abx-198 VL 187 CAGCAGTATGGTAGCTCACCGCTCACT CDR3 79 Abx-221 VH 274 CGCTATGCCATGAAC CDR1 80 Abx-221 VHf 276 GGTATTAGTGGTAGTGGTGGTAGCACATACT CDR2 ACGCAGACTCCGTGAAGGGC 167 WO 2011/050242 PCT/US2010/053686 81 Abx-221 VH 278 GATCGCGATTTTTGGAGTGGTCCAfTTGACT CDR3 AC 82 Abx-221 VL 280 AGGGCCAGTCAGAGTGTTAGTAGAAGCTTA CDR1 GCC 83 Abx-221 VL 282 GGTGCATCCACCAGGGCCACT CDR2 84 Abx-221 VL 284 CAGCAGTATAATAACTGGATGTGCAGT CDR3 85 Abx-229 VH 189 CGCTATGCCATGAAC CDR1 86 Abx-229 VH 191 GGTATTAGTGGGAGTGGTGGTAGGACATAC CDR2 TACGCAGACTCCGTGAAGGGC 87 Abx-229 VH 193 GATCGCGATTTTGGAGTGGTCCATTTGACT CDR3 AC 88 Abx-229 VL 195 AGGGCCAGTCAGAGTGTTAGTAGAAACTTA CDR1 GCC 89 Abx-229 VL 197 GGTGCATCCACCAGGGCCACT CDR2 90 Abx-229 VL 199 CACCAGTATAGTAACTGGATGTGCAGT CDR3 91 Abx-338 VH 201 AGTTACTACTGGAGC CDR1 92 Abx-338 VH 203 CGTATTTATATCAGTGGGAGGACCACCTTCA CDR2 ACCCCTCCCTCAAGAGT 168 WO 2011/050242 PCT/US2010/053686 93 Abx-338 VHI 205 GATAGATATTATGGCTACCTTGACTAC CDR3 94 Abx-338 VL 207 AGGGCCAGTCAGAGTGTTAGCCGCAGTTACT CDR1 TAGCC 95 Abx-338 VL 209 GATGCATCCAGCAGGGCCACT CDR2 96 Abx-338 VL 211 CAGCAGTATGGTAGTTCACCGAGCACC CDR3 97 Abx-393 VH 213 CATTACTACTGGAGC CDR1 98 Abx-393 VII 215 TATATCTATTACAGTGGGAGCACCAACTACA CDR2 ACCTCTCCCTCAAGAGT 99 Abx-393 VH 217 GGTATGGGCTTTGACTAC CDR3 100 Abx-393 VL 219 CGGGCAAGTCAGGCCATTAGAAATGATTTA CDR1 GGC 101 Abx-393 VL 221 TCTGCATCCAGTTTGCAAAGT CDR2 102 Abx-393 VL 223 CTACAGCATAATAGTTTCCCTCCGACG CDR3 [0402] Expression vectors were created as described above which contain coding sequence for both the heavy and light chain of each of mAb 5F9 and Abx-229. 169 WO 2011/050242 PCT/US2010/053686 [0403] The invention is illustrated by the following examples, which should not be construed as further limiting. EXAMPLES EXAMPLE 1: Generation of anti-GCC antibodies and characterization [0404] The generation of GCC protein for immunization and screening was performed as follows. GCC antigen was prepared by subcloning a portion of the GCC gene encoding a sequence comprising the following GCC sequence (signal sequence and extracellular domain) into an expression vector. MKTLLLDLALWSLLFQPGWLSFSSQVSQNCHNGSYEISVLMMGNSAFAEPLKNLEDAVNEGLEIVR GRLQNAGLNVTVNATFMYSDGLIHNSGDCRSSTCEGLDLLRKISNAQRMGCVLIGPSCTYSTFQMY LDTELSYPMISAGSFGLSCDYKETLTRLMSPARKLMYFLVNFWKTNDLPFKTYSWSTSYVYKNGTE TEDCFWYLNALEASVSYFSHELGFKVVLRQDKEFQDILMDHNRKSNVIIMCGGPEFLYKLKGDRAV AEDIVIILVDLFNDQYFBDNVTAPDYMKNVLVLTLSPGNSLLNSSFSRNLSPTKRDFALAYLNGILLF GHMLKIFLENGENITTPKFAHAFRNLTFEGYDGPVTLDDWGDVDSTMVLLYTSVDTKKYKVLLTY DTHVNKTYPVDMSPTFTWKNSKL (SEQ ID NO:229) [04051 The expression vector (pLKTOK107) provided a C-terminal IgGlFc region to fuse with the GCC sequence. This vector comprised an exon with the IgGI hinge, CH2 and CH3 domains, mutated to eliminate an unpaired cysteine from the CHI fragment in the exon. This IgGlFc region was further mutated at lysine 235 and glycine 237 to alanines. The construct was expressed recombinantly in human embryonic kidney (HEK) 293 cells transfected with the gene for SV40 T-antigen as secreted GCC sequence (amino acid residues 24 to 430 of SEQ ID NO:228) fused to a C-terminal human IgGl Fc. The protein, named TOK107-big (alt name hGCC-ECD/hIgGI Fc, SEQ ID NO:317), was purified by protein A chromatography and size exclusion chromatography. [04061 GCC antigen was also prepared by subcloning the above fusion protein into an expression vector such as pLKTOKI 11, which allows for fusion of the murine IgG2a transmembrane region onto the C-terminus. When this construct is expressed recombinantly in CHO cells, the GCC extracellular domain is detected on the cell surface. High cell surface expression of the GCC-Ig fusion protein (SEQ ID NO:318) is achieved when the pLKTOK 111 vector is co-transfected with pLKTOKI 23, which comprises 170 WO 2011/050242 PCT/US2010/053686 marine CD79a (MB-1) and CD79b (B29). Clone #27 from this transfection (CHO GCC#27) was used as immunogen. HT-29-GCC#2 cells also were used as immunogen. [0407] For screening of hybridoma supernatants and purified mAbs by ELISA, the nucleic acid encoding a GCC fusion construct was cloned into a pCMV1 expression vector (Sigma). Purification tags: FLAG-tag (in the N-terminus) and His-tag (in the C-terminus) were cloned into the construct as well. The fusion protein construct was transfected into 293 cells, expressed, and recombinant protein was purified over and Anti-FLAG@ M2 Agarose Affinity column (Sigma). [0408] Reagents and Cell lines. HEK293 cells, CHO, and T84 human colon cancer cells and were obtained from ATCC and maintained according to ATCC protocols. [0409] Mice: Female C57BL/6 mice, 4-6 weeks old, were purchased from Taconic Farms, Inc. (Germantown, NY) for the generation of the murine hybridomas. Xenomice, bred in-house until 4-6 weeks old, producing human IgG2 antibodies were obtained from Abgenix, Inc. (Fremont, CA) for the generation of the human hybridomas. All animals were acquired and maintained according to the guidelines of the Institutional Animal Care and Use Committee of Millennium Pharmaceuticals, Inc. [04101 Cell Lines: The cell lines used for functional assays were cell pairs of GCC transfected cells and vector control HEK293 or HT29 cells. HT29 cells were transfected with the full length GCC under control of the EF-la promoter or empty vector (pLKTOK4) and selected in G418. The GCC in these cells was confirmed to have a cGMP response when contacted with the ST peptide (1-18 or 5-18). HEK293 cells were transfected full length GCC under control of the CMV promoter or empty vector (pN8mycSV40) and selected in blasticidin. The GCC in these cells has a myc tag. The clones selected for highest GCC expression were 293-GCC#2, HT29-GCC#2 and HT29 GCC#5. The HT29-GCC#2 also were used as immunogens for generating anti-GCC antibody molecules. Additional GCC-expressing cells are CT26 cells. To develop the GCC-expressing CT26 cell line, pTOK58D vector was used. Full length GCC was cloned into the site normally used for heavy chain cloning and luciferase was cloned into the site normally used for light chain cloning. After transfection into CT26 cells, independent expression of both GCC and luciferase was confirmed. Surface expression of GCC was confirmed by flow cytometry using the 5F9 antibody. Clone #32 was selected for further studies. 171 WO 2011/050242 PCTJUS2010/053686 [0411] The T84 colon cancer cell line endogenously expresses GCC. Taqman analysis of GCC in a broad cell line panel revealed that T84 was the only cell line that express mRNA for GCC. Staining for GCC with a GCC selective mAb on cell pellets of T84 cells showed significant GCC protein expression. [0412] Quantitation of GCC receptor levels with radiolabeled ligand (ST-toxin) suggested that the 293-GCC#2 cell expressed more GCC than T84 cells while the HT29 GCC#2 or #5 expressed the fewest GCC molecules per cell. Cell line Whole cell binding assay (receptor /cell) HT-29-GCC#2/#5 100,000 T84 endogenous GCC 300,000 293-GCC 600,000 [0413] Generation of murine mAbs by protein immunization: Human GCC extracellular domain/human Ig fusion protein (TOK107-hIg, 50 pg) was suspended in Dulbecco's phosphate buffered saline (PBS; GIBCO, Grand Island, NY) and emulsified with an equal volume of complete Freund's adjuvant (Sigma Chemical Co., St. Louis, MO), C57BL/6 mice were immunized by injection of the emulsion at three subcutaneous sites and one intraperitoneal (i.p.) site. Two weeks after the initial immunization, the mice were given a booster immunization i.p. with 25 pg TOK107-hIg in incomplete Freund's adjuvant. One week later, a small amount of blood was collected from the tail vein and the serum binding activity against TOK107-Ig was titered by ELISA. Mice were selected for fusion when their titer exceeded 1:24,300 by ELISA or 1:500 by FACS. The selected mice were boosted by injection of 25 gg TOK107-hIg in PBS. Four days later, one mouse was euthanized and a spleen cell suspension was prepared and washed with PBS for fusion with P3 cells. One month later, spleen cells from another mouse were prepared for fusion with P3 cells. The fused cells were tested for production of antibodies which specifically bound to GCC by ELISA for binding TOK 07-hIg compared to a nonGCC antigen or to the Fc region of IgG and by FACS for binding to T84 cells, or Caco-2 cells or HT-29 clone #2 cells compared to vector control and compared to non-GCC-expressing MCF-7 cells. Isotype was determined using ISOSTRIP@ mouse monoclonal antibody isotyping kit 172 WO 2011/050242 PCT/US2010/053686 (Roche Diagnostics Mannheim Germany). These immunization schemes and hybridoma fusions produced the 1D3, 8E12, 3G1 and 1OB8 murine anti-GCC antibody molecules. [04141 Generation of human mAbs. XENOMOUSE genetically engineered mice (Abgenix, Fremont, CA) (8 to 10 weeks old) were immunized for production of human monoclonal antibodies. See, Mendez et al. Nature Genetics 15:146-156 (1997), Green and Jakobovits J. Exp. Med. 188:483-495 (1998). Several immunization schemes were employed. In one scheme, one hundred micrograms of human GC-C extracellular domain/human Ig fusion protein (TOK107-hlg) were suspended in Dulbecco's phosphate buffered saline (PBS; GIBCO, Grand Island, NY) and emulsified with an equal volume of complete Freund's adjuvant (Sigma Chemical Co., St. Louis, MO). XENOMO USEu were immunized by injection of the emulsion at three subcutaneous sites, base of tail and one intraperitaneal (i.p.) site. Fourteen days after the initial immunization, the mice were given a booster immunization with 50 jg TOK107-hlg in incomplete Freund's adjuvant Sera testing indicated insufficient titer so after a few weeks rest, a second booster of 50 pg human TOK107-hlg was given. Two weeks later, a small amount of blood was collected from the tail vein and the serum activity against TOK107-Ig was titered by ELISA and against HT29-GCC#2 cells by FACS. Mice were selected for fusion when their titer exceeded 1:24,300 by ELISA or 1:500 by FACS. Nearly three months after that boost, mice were boosted with 10 7 HT-29 #2 cells and the next day boosted with 50 gg TOK107 hg, both in incomplete Freund's adjuvant. A mouse immunized with this scheme produced the 5F9 and the lD2 human anti-GCC antibody molecules. A mouse immunized with this scheme produced the 5F9 and the 1D2 human anti-GCC antibody molecules. Four days later, the mice were euthanized and spleen cell suspensions were prepared and washed with PBS for the fusion. The fused cells were tested for production of antibodies which specifically bound to GCC by ELISA for binding TOK107-hIg compared to a nonGCC antigen or to the Fc region of IgG and by FACS for binding to T84 cells or HT 29 clone #2 cells compared to vector control and compared to non-GCC-expressing MCF 7 cells. Isotype was determined using ELISA or by FACS using IgG or IgM specific secondary antibodies. A mouse immunized with this scheme produced the 5F9 and the 1D2 human anti-GCC antibody molecules. 173 WO 2011/050242 PCT/US2010/053686 [0415] In another scheme, CHO-GCC#27 cells (5 x 106) comprising the pkTOK 111 vector and expressing the GCC extracellular domain on their surfaces were used as the immunogen two times with two weeks between BIP (base of tail + intraperitoneal) immunizations. After sampling blood to identify anti-GCC reactivity by ELISA against TOK107-hlg, the mice were boosted with HT-29 GCC #2 cells (either three weeks or more than two months since the previous boost). Four days after the last boost, the respective spleen was harvested for cell fusion. The fused cells were tested for production of antibodies which specifically bound to GCC by ELISA for binding TOK107-hg compared to a nonGCC antigen or to the Fc region of IgG and by FACS for binding to HT-29 clone #2 cells compared to vector control, T84 cells or non-GCC expressing MCF-7 cells. Isotype was determined using ELISA. These immunization schemes and hybridoma fusions produced the 5113, 6H8, 8C2, 10C10, 10D3 and 1C9 human anti-GCC antibody molecules. [0416] Hybridomas that produce human mAb: Spleen cells were counted and mixed with SP 2/0 myeloma cells (ATCC No. CRL8-006, Rockville, MD) that are incapable of secreting either heavy or light chain immunoglobulin chains at a spleen:myeloma ratio of 2:1. Cells were fused with polyethylene glycol 1450 (ATCC) in 12 96-well tissue culture plates in HAT selection medium according to standard procedures. Between 10 and 21 days after fusion, hybridoma colonies became visible and culture supernatants were harvested then screened by ELISA and FACS. [0417] SLAM technology-based antibody generation: Monoclonal antibodies were also isolated by Abgenix's SLAM technology (Babcook et al PNAS 93:7843-7848 (1996)). The initial step in this method was to immunize XENOMOUSE mice were immunized with GCC antigen by a scheme among those described below. Then the SLAM (Selected Lymphocyte Antibody Method) step involves first identifying within a large population of lymphoid cells a single lymphocyte that is producing an antibody with a desired specificity or function, and then rescuing from that lymphocyte the genetic information that encodes the specificity of the antibody. The variable region of such a lymphocyte (initially producing an IgG2 or IgG4 antibody) is amplified and transferred to a vector bearing the IgGI isotype. 174 WO 2011/050242 PCT/US2010/053686 [0418] Immunization schemes for the SLAM antibodies (e.g., Abx-229, -012, -221, -020, -338, -106, -198 or -393) included using TOK-hIg or TOK-hIg conjugated to keyhole limpet hemocyanin. Immunizations were either through the foot pad or a combination of the base of the tail and intraperitoneal. The initial immunization of 10 pLg immunogen included either TITERMAX@ gold adjuvant or complete Freund's adjuvant. Six to eight boosts with 5 gg of immunogen were performed, using either Alum, TITERMAX@ gold adjuvant or incomplete Freund's adjuvant. If TITERMAX gold was the initial adjuvant, alum was the boost adjuvant and boosts were performed at 3 to 4 day intervals. If the initial immunization and boosts employed complete then incomplete Freund's adjuvants, intervals between boosts were performed at about two week intervals. Sera tests of titers at the fourth to sixth boost sometimes were followed by additional boosts. The final boost prior to harvest four days later employed the immunogen in PBS. [0419] Analysis of mAb by ELISA. High-protein binding 96-well EIA plates (Costar/Corning, Inc. Coming, NY) were coated with 50 pl/well of a 2 ptg/ml solution (0.1 gg/well) ofTOK107-hlg and incubated overnight at 4"C. The excess solution was aspirated and the plates were washed with PBS/0.05% Tween-20 (three times), then blocked with 1% bovine serum albumin (BSA, fraction V, Sigma Chemical Co., MO) for 1 hr at room temperature (RT) to inhibit non-specific binding. The BSA solution was removed and 50 gl/well of hybridoma supernatant from each fusion plate well were added. The plates were then incubated for 45 min. at 37 "C and washed three times with PBS/0.05% Tween-20. Horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti human IgG F(ab)2 (H&L) (Jackson Research Laboratories, Inc., West Grove, PA) diluted 1:4000 in 1% BSA/PBS was added to each well and then the plates were incubated for 45 min. at 37"C. After washing, 50 pl/well of ABTS solution (Zymed, South San Francisco, CA) was added. The intensity of the green color of positive wells at 405 nm was assessed on a Vmax microtitre plate reader (Molecular Devices Corp., Sunnyvale, CA). All hybridoma wells that gave a positive response were then expanded to 24-well cultures, subcloned by limiting dilution and analyzed by ELISA and FACS. The three best producing subclones were expanded further. [0420] Analysis of mAb by flow cytometry. Flow cytometry (FACS) screening was done on all the fusion plate supernatants in parallel to the ELISA screening. HT-29 clone #2 or untransfected HT-29 cells were grown in T225 flasks (Costar/Coming, Inc., 175 WO 2011/050242 PCT/US2010/053686 Corning, NY) in DMEM (GIBCO) supplemented 10% fetal bovine serum (GIBCO). Cells were detached from the flask surface using Versene (GIBCO), collected and washed twice with DMEM, then once with 1% BSA/PBS solution. The cells were re-suspended in 1% BSA/PBS and 2x 106 cells were added to each well of V-bottomed 96-well plates (Costar) and centrifuged for 5 min. at 2500 RPM (wash). The wash solution was discarded and 50 gl/well of supernatant from each fusion plate well wash added. A plate sealer (Linbro/MP Biomedicals, LLC, Solon, OH) was applied and the plates were then gently vortexed to resuspend and mix the cells with the supernatants and incubated at 4"C (on ice) for 30 min. The plates were then washed with cold 1% BSA/PBS (three times) and 50 pl/well FITC conjugated donkey anti-mouse IgG F(Ab)2 (H&L) or FITC-conjugated goat anti-human IgG F(Ab)2 (H&L) (Jackson) diluted 1:50 was added to each well for 30 min. at 4 0 C (on ice in dark). The plates were again washed three times in cold 1% BSA/PBS and fixed in cold 1% parafbrmaldehyde (Sigma)/PBS. The cells were transferred to cluster tubes (Costar) and analyzed on a FACScalibur flow cytometer (Becton Dickenson, San Jose, CA). Any hybridoma wells that showed a positive shift were then expanded to 24-well cultures, subcloned by limiting dilution. [0421] Internalization assay. Internalization of anti-GCC antibody molecules was tested in both GCC-expressing cells and vector control cells, using immunofluorescence microscopy. Cells were grown on coverslips and placed on ice for 10 minutes prior to incubation with 10 pg/mI antibody in cold culture medium for 20 minutes on ice. For internalization, antibody-containing medium was replaced with fresh culture medium and the cells were shifted to 37C for 2-3 hours or maintained on ice. After rinsing in PBS and a brief fixation in 4% paraformaldehyde at room temperature, cells were permeabilized for 15 min in 0/5% TRITON X-100. The localization of the test antibody was determined using a fluorescently labeled anti-IgG antibody by laser scanning confocal microscopy. Antibody molecules localized to the cell surface of GCC-expressing cells when on ice. Upon incubation at 37 0 C, 5F9 showed punctuate staining within the cell membrane, indicative of internalization. No internalization was detected with vector cells. [0422] Summary of Properties of Anti-GCC Antibody Molecules. Most of the antibodies generated herein were tested in a number of the assays described above. Table 7 summarizes the in vitro properties for each. (T84=human colon tumor cells, 176 WO 2011/050242 PCT/US2010/053686 MCF7=human breast tumor cells, WB=western blot, LP=immunoprecipitation, IHC=immunohistochemistry; internalization used T84 cells compared to MCF-7 cells) Table 7. Properties of anti-GCC antibody molecules ELISA FACS Ab TOK1 TOK8 IT- HT- T84 MCF7 WB IP IHC Intemali 07-hIg 2-hIg 29#2 29 zation 8F1 + + + + + 3G1 + - + - + - - + + + 10138 + - + - + - - + + 5H3 + - + - + - - + + + 6H8 + - + - + - - + + 8C2 + - + - + - - + + + 10C0 - - + - + - - + 10D3 + - + - + - + + 1D2 + - + - + - - + 4A12 + - + - + - - + 5F9 + - + - + - + + + + 1C9 + - + - + - - + Abx-012 + + - + + + Abx-015 + + + Abx-020 + + - + + Abx-106 + + + Abx-198 + + + + Abx-221 + + + + 177 WO 2011/050242 PCTUS2010/053686 Abx-229 + + + + Abx-252 + + + + Abx-338 + + + + Abx-393 + + - + [04231 Additionally, some antibodies were tested for their ability to inhibit the ST peptide-induced calcium ion flux in GCC-expressing cells. The cGMP assay was performed in HT29-GCC#18 cells in the presence of 50 nM ST in the presence or absence of anti-GCC antibody molecules. There was dose-dependent inhibition of the calcium ion flux by 5F9. Other antibodies, 5H3 and Abx-338 also inhibited the calcium ion flux induced by ST. [0424] Estimate of relative affinity of anti-GCC antibody molecules. The relative affinities (EC50; antibody concentration for half maximal binding) of some anti GCC antibody molecules were estimated from ELISA measurements against TOK107-hlg and by FACS measurements with GCC-expressing cells. The following table displays some results. Table 8. EC50 of anti-GCC antibody molecules Antibody EC50, TOK107-hg, M EC50, Cells, M 5F9 3.65 x 10-8 1.24 x 10-9 5H3 4.16 x 10- 9 4.7 x 10-7 Abx-338 4.9 x 101 9.0 x 10-1 3G1 2.28 x 10- 8 5.8 x 10 0 Abx-229 2.95 x 10 Abx-221 6.55 x 10- 9 Abx-020 4.58 x 10-9 Abx-012 5.55 x 10- 0 178 WO 2011/050242 PCT/US2010/05368 6 Abx-198 4.77 x 10 [0425] Measurement of affinity of anti-GCC antibody molecules. A BIACORETm T100 system (GE Healthcare, Piscataway, NJ) was used to measure the affinity of anti-GCC 5F9 antibody at 22*C. [0426] Step 1: MAb 5F9 (Prep A) was diluted to 20 pg/mL in 10 mM sodium acetate, pH 4.0 and Reference 5F9 MAb (Prep B) was diluted to 10 ptg/mL in 10 mM sodium acetate, pH 4.0. Each mAb was covalently immobilized to several CM4 BIACORE chips using standard amine coupling. For each CM4 chip prepared, Prep A 5F9 was immobilized over two flow cells at around 75-100 RU while Prep B 5F9 was immobilized to one flow cell at around 70-80 RU. The remaining fourth flow cell of each CM4 chip was used as the reference flow-cell. [0427] Step 2: The stock concentration of GCC-ECD-Fc (TOK107-hg) was determined using the methods detailed by Pace et at. in Protein Science, 4:2411 (1995), and Pace and Grimsley in Current Protocols in Protein Science 3.1.1-3.1.9 (2003). [0428] Step 3: For each prepared CM4 chip described in Step 1, GCC-ECD-Fc was injected for 2 minutes at a concentration range of 202 nM - 1.6 nM (2x serial dilution) followed by a 7 minute dissociation. Samples were randomly injected in triplicate with several buffer inject cycles interspersed for double referencing. To obtain more significant off-rate decay data, three additional 101 nM GCC- ECD-Fc injections and three additional buffer injections were performed with a 2 minute injection and a 4 hour dissociation time. A flow rate of 100 pL/min was used for all experiments and all surfaces were regenerated with a 20 second pulse of 10mM Glycine-HCl (pH 2.0). All samples were prepared in the running buffer which was Hepes-buffered saline, 0.005% polysorbate 20, pH 7.4 (HBS-P) with 100 pg/mL of BSA added. [0429] Step 4: All sensorgram (plot of surface plasmon resonance vs time) data were processed with Scrubber 2.0 software (BioLogic Software, Campbell, Australia) and globally fit to a 1:1 interaction model including a term for the mass transport constant km using CLAMPTM software (Myszka and Morton Trends Biochem. Sci. 23:149-150 (1998)). [0430] The 1:1 model provided a very good fit to the data as long as the mAb immobilization levels were kept low enough so that the R, 8 , resulting from the global 179 WO 2011/050242 PCT/US2010/053686 analysis of the sensorgram data was at least below 12 RU for each surface. In most cases, one of the two Prep A 5F9 surfaces had an Rma too low (below 2 RU) for reliable kinetic measurements. Data from two flow cells of GCC-ECD-Fc binding to Prp A 5F9 from the same CM4 chip were simultaneously fit whenever possible, however. When mAb surfaces were prepared resulting in a higher R., (> 12 RU), sensorgrams clearly showed complex kinetics and thus a 1:1 model fit the data poorly. This isn't surprising due to the fact that GCC-ECD-Fc is a bivalent construct and a higher surface density of immobilized mAb most likely increases the probability that the GCC-ECD-Fc binds avidly to the surface. Replicates reported for this study include only those data that fit well to the 1:1 interaction model. The resulting KD's and rate constants of all replicates for Prep A 5F9 and the Prep B reference mAb are listed in Table 9 and Table 10, respectively. Table 9: GCC-Fc binding to immobilized Prep A 5F9 mAb Replicate Rmax ka (M-is-1) kd (s') KD (pM) (RU) A 11 1.06 X 10 5 1.19 X 10- 5 112 B 8 1.20 X 10 5 1.10 X 10- 5 91.7 C 5 1.07 X 105 2.15 X 10- 5 201 D 9 1.22 X 10 5 1.11 X 10 91.0 E 6,4 9.64 X 10 4 1.77 X 10- 5 184 Avg. (95% Conf. Int.) 1.10 (0.13) X 10 5 1.46 (0.59) X 10-- 136 (65) Table 10: GCC-Fc binding to immobilized Prep B 5F9 mAb Replicate Ra k. (M's') k4 (s') KD (pM) (RU) F 9 9.68 X 104 7.64 X 10-' 78.9 G 8 1.20 X 10 5 1.24 X 10- 5 103 H 7 9.09 X 104 9.57 X 10-6 105 1 12 1.21 X 10 5 1.54 X 10 5 127 180 WO 2011/050242 PCT/lJS2010/053686 Avg. (95% Conf. Int.) 1.07 (0.25) X 10 5 1.13 (0.54) X 10- 5 103 (31) Conjugation of Toxins to Antibodies [0431] Maytansines. Mouse anti-human (MAH)-IgG-DMI, and anti-GCC-DM1 were generated according to a one-step process for the production of cytotoxic conjugates of maytansinoids as described in U.S. Patent No. 6,441,163. [04321 Briefly, maytansinoids were conjugated to antibodies using published procedures. DM1 was conjugated to antibodies using SMCC heterobifunctional crosslinker (succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate; Chari et al. Cancer Research 52:127-131 (1992). DM4 was conjugated to antibodies using SPDB heterobifunctional crosslinker (Widdison et al. J Med. Chem. 49:4392-4408 (2006)). Conjugated antibody is separated from unreacted reaction by-products by gel filtration chromatography using a SEPHADEXTM G-25 column. [0433] Auristatins. Conjugation by auristatins can be performed using published procedures (e.g., Doronina et aL, Nature Biotech., 21: 778-784 (2003)). In general, auristatins are linked to cysteines of antibody chains. Linkage to cysteines is accomplished first by reduction of disulfide bonds in the antibody molecule. Control of the reduction process seeks to limit the reduction to some, but not necessarily all, interchain disulfide bonds. Consequently, auristatins are able to bind at the free cysteines. Quenching of the conjugation reaction is followed by removal of reaction by-products and buffer exchange to the desired formulation. [0434] In brief, an anti-GCC antibody molecule at 7.6 mg/mL is pre-equilibrated at 37*C, and then a 15% volume of 500 mM sodium borate, pH 8.0 is added to raise the pH to 7.5-8.0. The solution also contains 1 mM DTPA. The antibody is partially reduced by adding 2.6 equivalents of tris(2-carboxyethyl)phosphine (TCEP) per mole of anti-GCC antibody molecule and stirring at 37 *C. After 28 minutes, the solution of reduced anti GCC antibody molecule is placed on ice, then treated immediately with 4.8-4.9 molar equivalents (relative to anti-GCC antibody molecule) of drug linker (e.g., mc-vc-MMAF or mc-vc-MMAE or mc-MMAF) as a 20.5 mM solution in DMSO. Additional DMSO is 181 WO 2011/050242 PCT/US2010/053686 introduced to bring the mixture to 10% DMSO by volume. The reaction mixture is stirred on ice for -90 minutes before treatment with a 5-fold molar excess of N-acetyl cysteine (relative to mc-vc-MMAF). The conjugate is isolated by tangential flow filtration, first being concentrated to -10 mg/mL, then diafiltered with ~10 diavolumes of PBS. The resulting antibody drug conjugates had an average drug loading of about four drug-linker units per antibody. For convenience, in the following Examples and attached Figures, auristatin immunoconjugates are referred to in the following abbreviated format, irrespective of drug loading: "Ab-vc-MMAF" refers to an anti-GCC antibody molecule conjugated with mc-vc-MMAF; "Ab-vc-MMAE" refers to an anti-GCC antibody molecule conjugated with mc-vc-MMAE; and "Ab-mc-MMAF" refers to an anti-GCC antibody molecule conjugated with mc-MMAF. Immunoconjugates comprising specific anti-GCC antibody molecules are referred to in the same format, .e.g., 5F9-vc-MMAF, 5F9-vc MMAE, and 5F9-mc-MMAF. [0435] To prepare antibody drug conjugates with an average drug loading of about two drug-linker units per antibody, the protocol (above) is modified by reducing the amount of TCEP by 50%. The amount of drug linker is also reduced by 50%. The corresponding antibody drug conjugate is abbreviated as Ab-vc-MMAF(2). Preparation of 5F9 vcMMAE [0436] Using a method similar to the general method set forth above, the 5F9 mAb was conjugated to an auristatin derivative designated MMAE (Formula (XII1)) using a vc (Val-Cit) linker described herein to create the immunoconjugate designated 5F9 vcMMAE. The conjugation of the vc linker to MMAE (Seattle Genetics, Inc., Bothell, WA) was completed as previously described (see, e.g., US 2006/0074008). [04371 Briefly, a 17.8 mg/mL solution of the 5F9 niAb in 100 mM acetate at pH 5.8 was adjusted to pH 8 with 0.3 M sodium phosphate dibasic, yielding a final mAb concentration of 11.3 mg/ml. Then, DTPA was added for a 1 mM final concentration in the reaction mixture. The mAb was then partially reduced by adding 2.28 molar equivalents of TCEP (relative to moles of mAb), and then stirred at 37"C for 1.5 hours. The partially reduced mAb solution was then cooled to 4"C, and 4.4 molar equivalents of vcMMAE (relative to moles of antibody) were added as a 20.3 mM solution in DMSO. The mixture was stirred for 30 minutes at 22*C, then for 15 additional minutes following the addition of 5 molar equivalents of N-acetylcysteine (relative to moles of vcMMAE). 182 WO 2011/050242 PCTUS2010/053686 Excess quenched vcMMAE and other reaction components were removed by ultrafiltration/diafiltration of the immunoconjugate with 10 diavolumes of PBS, pH 7.4. The resulting immunoconjugate was designated 5F9 vcMMAE and has the following formula: A b NHO
H
2 N 0 where Ab is the 5F9 mAb, and in is form 1 to 8. The average drug loading (mn) was about 3.6. [0438] Cytotoxicity assays. To measure each antibody's ability to bind, internalize and kill target expressing cells, cytotoxicity assays were performed. in this assay, cells were incubated with various concentrations of the unconjugated primary anti-GCC antibody and a fixed non-toxic concentration of DM1-conjugated anti-human Fc secondary antibody(indirect cytotoxicity) or with various concentrations of toxin conjugated anti GCC mAb (direct cytotoxicity). Cell viability was measured by WST assay after 4 days incubation. The relative potency of human anti-GCC antibodies on 293-GCC#2 cells is shown in Table 8 and was determined using a DM1 conjugated mouse anti-human IgG mAb (MAH-IgG was purified from clone HP607 (CRL1753, ATCC). 5F9 and 229 are the most potent anti-GCC mnAb with LD50's of 26 and 78 pM. Although not shown here, the error is generally within 20% of these averages, as measured by range of replicates or standard deviation of> 2 replicates. Table 11. Cytotoxicity assay results for anti-GCC antibodies on 293-GCC#2 cells Anti-GCC Ab LD50 (pM) 5F9 26 229 78 106 166 183 WO 2011/050242 PCT[US2010/053686 221 207 338 267 12 279 20 569 5H3 722 10D3 1596 8C2 2038 IOC1O 2443 1D2 >3055 6H8 >4818 393 >5400 198 >5400 [0439] Cell surface binding. Binding ofunconjugated 5F9 or 5F9 conjugated to auristatins, was evaluated by in indirect immunofluorescence assay using flow cytometry. 1 X 106 cells/ well were plated in a V-bottom 96 well plate and incubated on ice for 1 hour with serial antibody dilutions of 1-0.001 gg/ml. Cells were washed twice with 3% FBS in ice cold PBS and incubated with 1:200 mouse anti-human PE IgG (Southern Biotech 2043-09) for 1 hour on ice. Cells were washed again and analyzed by flow cytometry on a BD FACS Canto II flow cytometer. Data was analyzed using FACS Canto II system software and mean fluorescence intensities were determined. [0440] Epitope mapping. Multiple strategies were initiated to identify epitopes. Peptide array. 20-mer peptides, with 15 aa overlap were generated, covering the extracellular domain (ECD) into the transmembrane domain of GCC (aa 1-440). Peptides were synthesized and provided as arrays, immobilized on glass slides. Arrays were hybridized with each of the anti-GCC antibodies to determine if linear peptides were sufficient for binding. Abx-198 bound peptides 55 and 56, while 3GI, 8F1 and lOB8 antibodies bound peptides 55, 56 and 57. The sequence, 184 WO 2011/050242 PCT/US2010/053686 ILVDLFNDOYLEDNVTAPDYMKNVLVLTLS (SEQ ID NO:225) is spanned by these peptides. A region of overlap among the peptides is LEDNVTAPDY (SEQ ID NO:314). Abx-012, Abx-338 and Abx-106 bound peptides 71 and 72. The sequence, FAHAFRNLTFEGYDGPVTLDDWGDV (SEQ ID NO:226), and the sequence RNLTFEGYDGPVTLD (SEQ ID NO:315) overlaps the two peptides. [0441] Cell surface binding to GCC truncation mutants. Truncation mutants of the GCC ECD were generated (FL mature peptide and 8 truncations (A 1-32, A1-49, A1-94, A1-128, A1-177, A1-226, Al-279, A1-229 and Al-379), as FLAG tagged constructs (pFLAG-CMV-3), representing approximately 50 aa deletion increments. Constructs were expressed in 293 cells, followed by immunoprecipitation by the anti-GCC antibody molecule and Western blotting for the FLAG epitope in lysates of 293 cells transfected with the GCC ECD mutants. Antibody 5F9 binds cells with the AI-32 mutation, but not cells with the Al-49 mutation. The binding of 5F9 to GCC was lost when the protein is truncated between aa 33-50 suggesting that this region is involved in the recognition of 5F9 to its binding epitope on GCC. However, since the rat and mouse GCC sequences are identical to human GCC in this region, and 5F9 does not bind mouse or rat GCC, 5F9 antibody likely binds a conformational epitope formed by the presence of amino acids 33 to 50 of human GCC. EXAMPLE 2. Toxin-linker selection / ADC characterization [0442] In an antibody drug conjugate (ADC) strategy, the conjugation of highly potent toxins to antibodies, the cytotoxicity of the toxin can be directed to tumors in a target-specific manner, delivering the toxin to antigen expressing tumor cells without affecting antigen negative cells in normal tissues thus reducing systemic toxicity. Auristatin (analog of dolastatin 10) and maytansine class toxins were evaluated as ADCs with anti-GCC mAb. These toxins are all inhibitors of microtubule polymerization, acting as anti-mitotics. Tests in which cells were contacted with free toxins indicated that the cytotoxicity of free toxins did not distinguish between celts with GCC expression and control cells without GCC. These free toxins were potent against the 293-vector, 293 GCC#2 cellsin HT29-vector vs. HT29-GCC#5 cells as shown in Table 9. 185 WO 2011/050242 PCT/US2010/053686 Table 12. Cytotoxicity of free toxins MMAE MMAF DM1 DM4 Cell line LD50 SD LD50 SD LD50 SD LD50 SD 293 vector 0.07 6 3.37 2.34 2.83 2.00 0.79 0.79 293 GCC#2 4 3 2.07 1.48 2.96 2.23 0.83 1.03 HT29 4 1 5.21 1.91 2.69 1.09 0.57 0.27 HT29 GCC#5 7 4 7.65 3.32 1.00 0.14 0.40 0.17 [04431 The chemistry by which the different toxins are conjugated to antibodies differs and affects linker stability. Linker stability affects the therapeutic window by impacting drug release in the blood or nontarget tissues vs. drug release at the tumor. The ideal ADC linker has high stability while in the blood, but efficient release upon target mediated cell entry. Auristatins [0444] Three auristatin-linker pairs were evaluated. To first evaluate these conjugates in vitro and then determine which toxin-linker for large scale in vivo studies, 5F9 was conjugated to vcMMAE, vcMMAF and mcMMAF (20mgs per conjugate). [0445] Auristatins are synthetic toxins related to the natural product dolastatin 10. MMAE and MMAF differ subtly, with the MMAF form having a carboxylic acid group in the R2 position, reducing cell permeability and potency as free toxin. MMAE is a Pgp drug pump substrate, while MMAF is not. [0446] Auristatins are conjugated to interchain cysteines through a process of partial antibody reduction, reaction with a maleimido drug derivative, quenching with excess cysteine, concentration and buffer exchange into PBS. The auristatins can be attached with a cathepsin B sensitive dipeptide linker, which is cleaved upon cellular uptake, or with a noncleavable linker. [0447] In the vcMonoMethylAuristatin linker, a valine citrulline dipeptide linkage is attached to the drug through a p-amino benzyl carbamate (PAB) group and to the 186 WO 2011/050242 PCT/US2010/053686 antibody through a maleidimido caproyl conjugation group. Upon internalization, the dipeptide tinker is cleaved by the lysosomal protease cathepsin B, the PAB group self destructs, and free toxin is released. This linker was designed to maintain serum stability while maximizing intracellular drug release by cathepsin B. [0448] Auristatins can also be linked to antibodies through noncleavable linkers such as MMAF directly attached to the maleimido conjugation group, with no peptidase sensitive linker. MC conjugated ADC's are also effective at target mediated cell kill. [0449] The mechanism of drug release for noncleavable auristatin conjugates is thought to be through general antibody degradation in lysosomes. Through LC/MS studies it has been reported that Ab-mcMMAF conjugates release toxin in the form of a single cysteine-adduct. Binding of antibody drug coniugates [04501 All of the 5F9 antibody drug conjugates bound to 293-GCC#2 cells equally well. Table 10 shows the mean fluorescence intensity of 5F9 conjugates at increasing concentrations 293-GCC#2 cells. Other studies determined that the 5F9-SPDB-DM4 conjugate bound 293-GCC#2 cells in a concentration-dependent manner, whereas the 209 SPDB-DM4 antibody did not bind. Table 13. Chart of MFIs from binding assay of 5F9 and 5F9 conjugates in 293 GCC #2 cells: pg/m1 of 5F9 or 5F9 conjugate 0.001 0.004 0.016 0.063 0.25 1 5F9 00 937 465 615 7816 8026 vcMMAE 45 696 1787 854 7296 416 vcMMAF 40 707 1502 830 7563 779 cMMAF 83 776 106 5353 7398 585 187 WO 2011/050242 PCT/US2010/053686 104511 The 5F9-auristatin toxin conjugates were tested in direct cytotoxicity assays of a variety of cells which been transfected with GCC nucleic acid and selected for expression of GCC. A survey of the level of surface expression of GCC found that 293 GCC#2 cells express high amounts of GCC; HT 29 #2 and CT 26 #2.5 cells express GCC at intermediate to low levels; CT 26 #32 cells express GCC at high levels; and FIT 29 GCC #5 and HT 29 GCC #18 express low amounts of GCC. Table 11 shows a compilation of multiple studies yielding cytotoxicity data for the three auristatin conjugates in cells expressing target, or in wild-type cells or vector control cells. Target enhanced killing is observed in all cases of 5F9-conjugated toxins on 293 GCC #2 cells, with a greatly increased window when using MMAF vs. MMAE. Both the cleavable and noncleavable forms of MMAF were similarly potent. As a negative control, antibody drug conjugates were also made with sc209 antibody, which is a human IgGi monoclonal antibody raised against an unrelated target, with no reactivity to GCC. Direct cytotoxicity assays with 209 ADCs vs. 5P9 vcMMAF ADC showed target enhanced cell kill in the 293 cell model and the HT29 cell model. A comparison of 5F9-conjugated toxin activity among the cell lines indicates that the level of cytotoxicity has some correlation with the amount of GCC expressed by the cells. These data suggest that at least some cell lines expressing the most GCC were more susceptible to the cytotoxic activity of the conjugate than were cells expressing lower amounts of GCC. See Example 1 for relative GCC numbers per cell. Another factor in differences of cytotoxicity levels may be differences in internalization or intracellular processing of the conjugates, which may vary among wild type cell lines. Table 14. Cytotoxicity of anti-GCC auristatin ADCs. D50 (nM) of 209 LD 50 (nIM) of 5F9 conjugates conjugates Cell line vceMMAE veMMAF mcEMMAF 293 Vector 128 > 10 > 10 293 GCC # 2 0.37 0.001 0.002 293 Vector 1.8 >10 >10 293 GCC # 2 0.13 0.005 0.007 188 WO 2011/050242 PCT/U1S2010/053686 293 Vector >10 >10 293 GCC # 2 0.0004 >10 HT29WT 84 >10 >10 HT 29 GCC #2 24.1 0.127 3.1 CT26WT >500 >10 >10 CT26GCC#2.5 >500 >10 >10 CT 26 GCC #32 267 0.004 0.064 HT 29 vector 520.6 > 10,000 > 10,000 H1T 29 GCC #5 653.5 563.2 > 10,000 HT 29 GCC #18 554.6 > 10,000 > 10,000 HT29 0.93 >10 >10 HT29 GCC#5 0.59 0.32 >10 HT29 >10 >10 HT29 GCC#5 0.035 >10 [0452] If these potencies translate in vivo, with equal efficacy between mc and vcMMAF, the higher predicted MTD for mcMMAF would suggest a larger therapeutic window for this conjugate. Maytansines [0453] The 5F9-maytansine ADCs showed potent target enhanced killing in the 293-GCC#2 cells (Table 12). Interestingly, the 5F9-maytansine conjugates both showed target enhanced killing in the 293 model, however no target enhanced killing was observed in the HT29 model, raising concern about the utility of this model for in vivo evaluation of the maytansine conjugates. Again, the reasons for this difference are not readily explicable, but may be attributed to different receptor densities and/or differences in the internalization or processing of the conjugates within cells. As a negative control, 189 WO 20111050242 PCT/US2010/053686 antibody drug conjugates were also made with sc209 antibody, which is a human IgGI monoclonal antibody to an unrelated target, with no reactivity to GCC. Direct cytotoxicity assays with 209 ADCs vs. 5F9 ADCs show target enhanced cell kill in the 293 cell model with all 5F9 conjugates. In the HT29 cell model, 5F9-DMx conjugates and 209-DMx conjugates kill equally well, indicating a non-target specific mechanism of killing in those cells. Table 15 Cytotoxicity of anti-GCC maytansine ADCs LD50 (nM) Cell line 5F9-SMCC- 209-SMCC- 5F9-SPDB- 209-SPDB DMI DMI DM4 DM4 293 vector 25.3 10.4 293 GCC#2 <0.004 <0.004 293 vector 32 11 13 7.4 293 GCC#2 <0.000004 6.7 <0.000004 4.8 HT29 22.4 7.7 HT29 GCC#5 20.8 9.1 HT29 22 12 4.8 2.1 HT29 GCC#5 30 22 5.8 4.2 EXAMPLE 3: In vivo evaluation Tumor models: [04541 In vivo cytotoxicity of 5F9 ADCs were evaluated in mouse xenograft models. The initial in vivo work was done with the HT29-GCC#5 and #18 cell lines. The 293-GCC#2 cell line also was tested for in vivo growth and was developed as a serially transplantable trocar model. 190 WO 2011/050242 PCT/US2010/053686 [0455] To address the question of whether the GCC expression level in the xenograft model was relevant to the level of GCC expression in patients with metastatic colon cancer, GCC expression levels were compared by IHC analyses of xenograft tissue, human primary colon tumors and metastases. A panel of fresh frozen cell lines and tissues were utilized for GCC quantitation by rIHC with a mouse mAb to GCC 3G1. For IHC quantitation, scoring was done using a semi-quantitative 0-3 score system. If tumor model GCC levels < clinical GCC levels, modeling will likely be accurate or overestimate the exposure needed clinically. If tumor model GCC levels > clinical GCC levels, modeling may underestimate the exposure needed clinically. [0456] While there was some variability in the expression of GCC in metastatic samples, expression by the HT29-GCC#5 and #18 cells was in the range of many of the metastatic samples. By IHC, the staining of GCC on the HT29-GCC#5 and #18 cells was equivalent or lower than on the metastatic cells. This data suggests that our tumor models express GCC at levels comparable to the levels found in clinical samples of metCRC. [04571 Table 16 represents the scintillation counts for various tissues harvested at the 192 hour timepoint with averages for three animals represented. 5F9 preferentially accumulated in HT29-GCC#5 tumors vs. HT29-vector tumors, while 209 did not show much differential accumulation. This result provided support that a 5F9 antibody drug conjugates could be expected to accumulate in GCC expressing tumors. In all other tissues evaluated, there was little difference in the levels of 5F9 vs. mAb 209 antibody accumulation. In vivo distribution of radiolabeled 5F9 in HT29-GCC#5 & HT29-vector tumor-bearing mice [0458] A radioimaging study in tumor bearing mice was performed to evaluate tumor targeting and in vivo biodistribution of the anti-GCC antibody 5F9 and a negative control antibody sc209 (human IgGI monoclonal antibody targeting an unrelated cell surface target). The antibodies were radiolabeled with "In using DTPA as a bifunctional chelator. The in vivo behavior, including tumor targeting and biodistribution in normal tissues over time, was investigated with a marine dual-tumor model with both GCC(-) and 191 WO 2011/050242 PCT/US2010/053686 GCC(+) tumors. In vivo images (SPECT/CT) were acquired, and tissue radioactivity counting was used to supplement the spatial resolution. [0459] Subcutaneous tumors were grown in nude mice, with HT29-vector tumors on the right and HT29-GCC#5 tumors on the left. Antibodies were dosed at 0.3 mCi= 15 jg per animal. There were three animals per group, and groups were harvested at lh, 24h, 48h, 72h, 120h and 192h. [0460] A survey of tissues from animals in the 192h group indicated that both 5F9 and 209 accumulated to a similar degree in most normal tissues (e.g., blood, heart, stomach, small intestine, large intestine, muscle and skin) and in HT29-vector control tumors. Ab 209 accumulated to slightly higher levels than 5F9 in liver and 5F9 accumulated to slightly higher levels than 209 in lungs, spleen and kidneys. In the HT29 GCC#5 tumors, 5F9 preferentially accumulated at levels more than two-fold higher than the 209 levels. This result provided support that a 5F9 antibody drug conjugates could be expected to accumulate in GCC expressing tumors. [04611 To understand the kinetics of antibody accumulation in tumors, tumor data for each antibody was obtained for all time points throughout the study. The only tissue that showed accumulation is the 5F9 antibody in the GCC expressing tumor. The radioactivity level in all other tissues remained relatively flat, with little difference between the 5F9 levels and 209 levels. 5F9 preferentially accumulated in HT29-GCC#5 tumors, while 209 did not show any accumulation. This result provided support that a 5F9 antibody drug conjugate could be expected to accumulate in GCC expressing tumors. 192 WO 2011/050242 PCT/US2010/053686 Table 16. Accumulation of "'In-labeled GCC specific mAb but not control mAb to tumors expressing GCC. Mean % ID 5F9 Mean % ID etr IgG 1Hr 2.816+/-0.133 2.494+/-0.167 24hr 3.057+/-0.107 3.010+/-0.630 72br 4.485+/-1.029 3.564+/-0.152 120br 5.162+/-1.012 3.412+/-0.048 192hr 6.550+/-1.015 2.782+/-0.085 [0462] Accumulation of radiolabeled 5F9 in GCC expressing tumors over 7 days supported a once weekly dosing schedule. Pilot efficacy studies in HT29-GCC#5 s.c. tumors [04631 Studies were performed to determine efficacious conjugates and dosage regimens in mice bearing HT29-GCC#5 tumors. Mice were dosed with single or multiple doses. These studies determined that there was toxicity at too frequent dosing at higher levels of toxin conjugate (e.g., 150 pg/kg 5F9vcMMAF on a q3d x 5 schedule). Another study determined that q 14d x 5 schedule was too infrequent in this model to allow some toxin conjugates to show significant efficacy vs. controls. Additionally, a PD study with maytansinoid-antibody conjugates in this model demonstrated dose-dependent phosphohistone accumulation only with the DM4 toxin, not the DM1 toxin. Another study in this model showed some tumor growth inhibition by the non-specific 209 -toxin conjugate. These results suggested that other in vivo models needed to be evaluated. PK/PD study with 5F9 ADCs in mice carrying 293-GCC#2 tumors. [04641 An alternative tumor model used 293-GCC#2 cells. A PD study for 5F9 ADCs in 293-GCC#2 tumor bearing mice was performed. Mice were dosed with single doses of 5F9vcMMAF at 75ug/kg or 150ug/kg and serum was taken at timepoints from 1 hr through 4 days for PD analysis of phosphohistone H3 to test antimitotic effects of the toxin on tumor cells. Phospho-histone H3 was detected by antibody (Upstate 193 WO 2011/050242 PCT/US2010/053686 Biotechnology, now Millipore, Billerica, MA) staining of paraffin embedded sections of tumors. The data in Table 17 shows that each of the ADCs caused a significant increase in the pH3 positive cell population in the tumors indicating that each of them at both 75 and 150 pg/kg toxin dose equivalents was able to reach the tumors and have the desired anti-mitotic effect on the tumor cells. Table 17. The PD response as assessed by arrest of cells in mitosis (% pH3 positive tumor cells) following a single iv dose of 5F9 ADCs. Average % Tumor cells pH3 positive SD Vehicle control 2.556801 2.37707 5F9-vcMMAE 75 pg/kg 1hr 4.525187 0.178882 4hr 2.551616 1.688255 8hr 4.243988 0.352938 24hr 9.8199 4.82057 48hr 8.692061 4.756786 96hr 8.628345 1.065456 5F9-vcMMAE 150 pg/kg lhr 3.334943 1.351667 4hr 2.78543 1.690216 8hr 4.575611 1.130484 24hr 13.78776 3.343155 194 WO 2011/050242 PCT/US2010/053686 48hr 14.26067 5.448921 96hr 14.67942 1.827724 5F9-vcMMAF 75 pg/kg 1hr 4.235245 0.617585 4hr 4.18364 0.846752 8hr 4,930098 0.54746 24hr 20.22484 2.453935 48hr 9.920771 3.788795 96hr 10.38187 1.896461 5F9-vcMMAF 150 pig/kg lhr 3.465674 1.341187 4hr 4.416646 0.807636 8hr 8.594385 4.005021 24hr 21.53718 7.25212 48hr 15.15814 4.28407 96hr 11.12288 2.150476 5F9-mcMMAF 75 pg/kg lhr 5.365582 1.14198 4hr 4.044478 0.992449 8hr 8.228597 3.098222 24hr 14.10734 1.611093 195 WO 2011/050242 PCT/US2010/053686 48hr 19.37223 8.146504 96hr 7.749388 1.180759 5F9-mcMMAF 150 pg/kg Thr 3.212482 0.509604 4hr 4.722554 1.577531 8hr 9.105349 5.963128 24hr 27.51416 10.96057 48hr 13.34043 3.414961 96hr 15.60917 3.386154 [0465] Similar studies measured the phosphohistone levels in 293-GCC#2 tumor bearing mice treated with 5F9vcMMAF, 5F9-SPDB-DM4 and 5F9-SMCC-DM1. Mice were dosed with single doses at 150ug/kg and serum was taken at timepoints from 1hr through 21 days for PK analysis of both total antibody and toxin-conjugated antibody. The percentage of phosphohistone H3 positive cells in 293-GCC#2 tumors increased in response to all three ADCS: 5F9vcMMAF, 5F9-SMCC-DM1 and 5F9-SPDB-DM4. Maximal phosphohistone H3 levels were 3- to 5-fold increased over baseline, with peaks at 24 hours post-injection. Efficacy study with 5F9vcMMAF and 5F9-DMx in 293-GCC#2 s.c. tumors [0466] 5F9-SPDB-DM4, 5F9-SMCC-DM1 and 5F9vcMMAF were tested for efficacy in the 293-GCC#2 tumor model at two doses (75 pg/kg and 150 pg /kg toxin), on a q 1 4d x 5 schedule. Specifically, this study included vehicle-treated control, Sc209-DM 1 (150 pg/kg DM1 eq), Sc209-DM4 (150 pg/kg DM4 eq), Sc209-vcMMAF (150 pg/kg MMAF eq), 5F9-DM1 (150 pg/kg DM1 eq), 5F9-DM1 (75 ig/kg DM1 eq), 5F9-DM4 (150 pg/kg DM4 eg), 5F9-DM4 (75 pig/kg DM4 eq), 5F9-vcMMAF (150 pg/kg MMAF 196 WO 2011/050242 PCTUS2010/053686 eq), and 5F9-vcMMAF (75 pg/kg MMAF eq). Taconic females mice bearing 293-GCC#2 cells (10 mice per group) were used. [0467] Figure 1 depicts tumor growth in 293-GCC#2 bearing SCID mice treated with 5F9vc-MMAF, -DM1, and -DM4 on a ql4d schedule. Dose-dependent efficacy was observed with 5F9-SPDB-DM4 in the 293-GCC#2 model, while the 209-SPDB-DM4 control had no effect. 5F9-SMCC-DM1 was also efficacious, however less so than 5F9 SPDB-DM4 at 150ug/kg. 5F9vcMMAF (75ug/kg and 150ug/kg) was the most efficacious, however 209vcMMAF also had some activity, Therefore at these doses and schedules, 5F9-SPDB-DM4 had the greatest efficacious differential from its control conjugate. Efficacy study with 5F9vcMMAF and 5F9-DMx in 293-GCC#2 s.c. tumors [04681 5F9-SPDB-DM4 and 5F9-SMCC-DMI were tested for efficacy in the 293 GCC#2 tumor model at two doses (75ug/kg and 150ug/kg toxin) on a q7d x 5 schedule. Specifically, this study included vehicle-treated control, 5F9 alone (15 mg/kg), DM1 (300 sg/kg), DM4 (300 sg/kg), Sc209-DM1 (150 pg/kg DMI eq), Sc209-DM4 (150 pg/kg DM4 eq), Sc209-vcMMAF (150 gg/kg MMAF eq), 5F9-DM1 (150 sg/kg DM1 eq), 5F9 DM1 (75 pg/kg DMI eq), 5F9-DM4 (150 pg/kg DM4 eq), and 5F9-DM4 (75 pig/kg DM4 eq). Taconic females mice bearing 293-GCC#2 cells (10 mice per group) were used. Efficacy study with auristatin conjugates in 293 GCC#2 tumors [0469] 293 GCC#2 tumor-bearing SCID mice were treated with 5F9 conjugates with ye MMAE, vcMMAF or mcMMAF at three doses in comparison with 209 conjugates of these toxins or with free toxins or vehicle control. Doses were administered iv on a q7d x 4 schedule. Tumors were harvested at days 3, 7, 10, 13 and 17. The tumors in mice treated with control reagents demonstrated a continual increase in volume. Tumors treated with 5F9 auristatin conjugates showed dose- and time-dependent inhibition of this tumor growth. Table 18 provides a summary of the results (TGI=tumor growth inhibition, T/C-treatment/control, TGD-tumor growth delay, CR/PR=complete response/partial response; p value=measure to judge statistical significance, NS=not significant). 197 WO 2011/050242 PCT/US2010/053686 Table 18. Analysis of Auristatin ADCs in 293 GCC#2 tumor-bearing mice. Groups TGI T/C TGD CR/PR P value 209-vcMMAE 300 pg/kg 24.5 0.76 0.9 0 0.38>0.05 NS 209-vcMMAF 150 pg/kg 29.4 0.71 1.4 0 0.27>0.05 NS 209-mcMMAE 150 pg/kg 36.5 0.63 1.4 0 0.15>0.05 NS Free MMAE 300 Ag/kg 35.6 0.64 2 0 0.18>0.05 NS Free mcMMAF 150 jg/kg -3.4 1.03 -1.3 0 0.73>0.05 NS 5F9-vcMMAE 300 pg/kg 96.9 0.03 9/10 PR <0.001 5F9-vcMMAE 150 pg/kg 83.5 0.17 2/10 PR <0.01 5F9-vcMMA F 150 pg/kg 97 0.03 9/10 PR <0.001 5F9-mcMMAF 150 pg/kg 97 0.03 9/10 PR <0.001 5F9-vcMMAE 75 pg/kg 54.5 0.45 4.4 0.01<p<0.05 5F9-vcMMAF 75 pg/kg 88.5 0.12 6/10 PR <0.001 5F9-mcMMAF 75 pg/kg 87.5 0.13 7/10 PR =0.001 5F9-vcMMAF 37.5 pg/kg 65.2 0.35 7.1 =0.01 5F9-mcMMAF 37.5 pg/kg 63.6 0.36 5.8 =0.01 [0470] All three of these ADCs were efficacious in the 293 GCC#2 model on a q7d schedule. 5F9-vcMMAF and 5F9-mcMMAF are more potent than 5F9-vcMMAE, which correlates with in vivo PD (pHisH3) and in vitro cytotoxicity data. Efficacy study with 5F9vcMMAF and 5F9-DMx in T84 s.c. tumors [0471] 5F9-SPDB-DM4 and 5F9-SMCC-DM1 were tested for efficacy in the T84 tumor model at two doses (75ug/kg and 150ug/kg toxin) on a q7d x 5 schedule. Specifically, this study included vehicle-treated control, 5F9 alone (15 mg/kg), DM1 (300 gg/kg), DM4 (300 jg/kg), Sc209-DM1 (150 Rg/kg DM1 eq), Sc209-DM4 (150 g/kg 198 WO 2011/050242 PCT/US2010/053686 DM4 eq), Sc209-vcMMAF (150 pg/kg MMAF eq), 5F9-DM1 (150 jig/kg DM1 eq), 5F9 DM1 (75 pg/kg DM1 eq), 5F9-DM4 (150 pg/kg DM4 eq), and 5F9-DM4 (75 sg/kg DM4 eq). Taconic females mice bearing T84 cells (10 mice per group) were used. Immunohistochemistry [0472] Detection and measurement of relative amounts of GCC in tissues such as biopsies and xenografts can be performed by immunohistochemistry. Frozen tissue sections are fixed in a 1:1 solution of acetone and methanol for 20 min. at room temperature. Slides are washed in 1 x PBS for 5 minutes. Slides are processed with an automatic staining device, such as Ventana Discovery XT automated stainer (Ventana Medical Systems, Tucson, AZ) using manufacturers suggested reaction buffer. Anti-GCC antibodies are diluted to 5 jxg/ml in 5% goat serum. For human anti-GCC antibodies, e.g., 5F9, detecting secondary antibody is a 1:500 solution of goat anti-human biotinylated antibody in Dako Protein block (Dako, Carpinteria, CA). After autostainer process the antibody reaction, the slides are removed from the autostainer, rinsed in reaction buffer, dehydrated through standard series until xylene and given coverslips with a xylene-based mounting media. Efficacy study of ADCs in a primary tumor model [0473] Models of primary colorectal carcinoma and gastric cancer are developed as subcutaneous tumors in mice. 5F9-vcMMAE and 5F9-mcMMAF ADCs are tested in PHTX- 1lc primary tumor-bearing mice. Doses are administered iv on a q7d x 4 schedule. Conjugates of toxins with 209 are administered to control mice. Antitumor Activity of ADCs in a primary tumor model [0474] Two similar studies were conducted to determine the in vivo antitumor activity of 5F9-vcMMAE and to compare the antitumor activity of 5F9-vcMMAE to free toxin MMAE and to non-immune vcMMAE antibody toxin conjugate (209-vcMMAE) in PHTX-9c primary human colon tumor xenograft mice at various doses and dosing schedules and to determine the re-growth kinetics following treatment. Female CB-17 SCID mice (eight weeks old) were inoculated subcutaneously (SC) into the flank with PHTX-9c tumor fragments (2 mm x 2 mm). Tumor growth was monitored twice per week using vernier calipers and the mean tumor volume was calculated using the formula (0.5 x 199 WO 2011/050242 PCT/US2010/053686 [length x width 2 ]). When the mean tumor volume reached approximately 150 mm 3 (Study A) or 160 mm 3 (Study B), animals were randomized into treatment groups (n = 10/group for Study A and n = 9/group fbr Study B). [0475] Mice were treated (Study A) on a once weekly (QW) dosing schedule (3 doses) with 0.938, 1.875, 3.75, or 7.5 mg/kg 5F9-vcMMAE intravenously (IV) fbr 20 days or controls, which included vehicle (0.9% saline), 0.075 or 0.15 mg/kg MMAE IV on a QW schedule, or 1.875 or 3.75 mg/kg 209-vcMMAE IV on QW dosing schedules for 20 Days. In the second study (Study B), mice were treated with 0.938, 1.875, 3.75, 7.5, or 10.0 mg/kg 5F9-vcMMAE IV on a QW schedule (3 doses) or 3.75 mg/kg IV on a twice weekly (BIW) schedule (6 doses), or controls including vehicle, 7.5 or 10 mg/kg 209 vcMMAE, or 0.135 or 0.18 mg/kg MMAE administered IV on a QW schedule for 20 days. Doses were administered on Days 1, 8, and 15 for the QW schedule and Days 1, 4, 8, 11, 15, and 18 for the BIW schedule. The dose of free MMAE was calculated to match the amount of MMAE in the immunoconjugate doses by the following rationale: The equivalent dose of MMAE is 1.8% of the MLN0264 dose. The equivalent dose of linker + MMAE is 4% of the 5F9-vcMMAE dose. These calculations are based on a mean 3.9 MMAE molecules per antibody and a free antibody molecular weight of 150 kD. Actual antibody molecular weight will vary slightly due to degree of glycosylation. [0476] Tumor volume and body weight were measured twice weekly and were continued beyond the treatment period to measure regrwoth kinetics, as evidenced by tumor growth delay (TGD). Tumor volume measurements were continued until tumor volume reached 10% of the body weight in a single mouse within a treatment group, at which time the group was terminated. The percentage of tumor growth inhibition (TGI) ([mean tumor volume of the control group - mean tumor volume of a treated group] / mean tumor volume of the control group; a T/C ratio) was determined on Day 20. The T/C ratios across a treatment group were compared to the T/C ratios of the control group using a two-tailed Welch's t-test. Because the entire group was terminated if one tumor reached the size limit (approx. 1000 mm 3 ), TGD could not be calculated for groups where the average regrowth was slow. [0477] The differences in the tumor growth trends over time between pairs of treatment groups were assessed using linear mixed effects regression models. These models account for the fact that each animal was measured at multiple time points. A 200 WO 2011/050242 PCT/US2010/053686 separate model was fit for each comparison, and the areas under the curve (AUC) for each treatment group were calculated using the predicted values from the model. The percent decrease in AUC (dAUC) relative to the reference group was then calculated. A statistically significant P value (< 0.05) suggests that the trends over time for the two treatment groups were different Results are summarized in Tables 19 and 20, below. [0478] Antitumor activity was observed in all 5F9-vcMMAE-treated groups in both studies and the effect was shown to be dose-dependent. The results of the two studies were comparable. In mice treated with 5F9-vcMMAE at 0.938 mg/kg, IV on a QW schedule, TGI was 20.7-21,4%, p value was <0.05 compared with vehicle group. In the 1.875 mg/kg treated group administered TV, on a QW schedule TGI was 41.3-44.7%, p value was <0.001. In 3.75 mg/kg treated group administered IV, on a QW schedule TGI was 65.3.-65.7% (p <0.001) compared with vehicle group. 5F9-vcMMAE administered at 7.5 mg/kg IV QW yielded a TGI of 84.1-84.3% (p<0.001) and 10 mg/kg IV QW (Study B only) yielded a TGI of 91.2% (p<0.001). When 3.75 mg/kg was administered TV on a BIW schedule (S), significant inhibition was observed with a TGI of 84.9% (p<0.001). [0479] Moderate antitumor activity was observed at the higher doses of 209 vcMMAE of 7.5 and 10.0 mg/kg with TGI of 35.7 and 45.4%, respectively (p<0.001) but low doses of 209-vcMMAE (1.875 and 3.75 mg/kg) exhibited no inhibition (p>0.05). The antitumor activity observed by the high 209-vcMMAE is likely due to nonspecific activity by the MMAE portion of the immunoconjugate. [0480] Administration of free toxin MMAE yielded mixed results: 0.075, 0.135, and 0.15 mg/kg administered TV QW yielded no tumor growth inhibition (p>0.05) but administration of 0.18 mg/kg IV QW resulted in TGI of 50.4% (p<0.001). [0481] The greatest maximum body weight loss observed during treatment period was 2.3% on day 7 in the free toxin 0.18 mg/kg MMAE group of Study B and the 0.938 mg/kg 5F9-vcMMAE group of the same study. This indicates the drug was well tolerated. [0482] Tumor volume measurements were continued beyond the treatment period until tumor volume reached 10% of the body weight in a single mouse within a treatment group and then the treatment group was terminated. In these studies, tumor re-growth appeared to be dose-dependent. 201 WO 20111050242 PCT/US2010/053686 Table 19. Study A results of treatment of primary human colon tumor Xenograft in SCID mice. Treatment Dose Method of TGI BW TGD (days) (mg/kg) Administration/ change /(or days frequency (mean until first maximum tumor percent) >1000 mm Vehicle 0 TV QW x 3 doses N/A -0.2 0 209-vcMMAE 1.875 TVQW x 3 doses -2.0 (p>0.05) 8.3 0.6 209-vcMMAE 3.75 IV QW x 3 doses 4.5 (p>0.05) 6.9 0.4 MMAE 0.075 TV QW x 3 doses 5.0 (p>0.05) 8.3 1.0 MMAE 0.15 IVQW x 3 doses 11.1 (p>0.05) 10.8 2.0 5F9-vcMMAE 0.938 IV QW x 3 doses 21.4 (p>0.05) 9.0 3.3 5F9-vcMMAE 1.875 IV QW x 3 doses 44.7 (p>0.001) 10.6 8.2 5F9-vcMMAE 3.75 IV QW x 3 doses 65.3 (p>O.001) 9.0 17.8 5F9-vcMMAE 7.5 TV Qw x 3 doses 84.1 (p>0.001) 7.5 (>58 days first tumor) Table 20. Study B results of treatment of primary human colon tumor Xenograft in SCID mice. Treatment Dose Method of TGI BW TGD (days) (mg/kg) Administration/ change /(or days frequency (mean until first maximum tumor percent) >1000 mn) Vehicle 0 IV QW x 3 doses N/A -1.8 0 209-vcMMAE 7.5 IV QW x 3 doses 35.7 (p>0.001) 4.2 4.2 209-vcMMAE 10.0 IV QW x 3 doses 45.4 (p>0.001) 2.6 11.3 MMAE 0.135 IV QW x 3 doses 2.2 (p>0.05) 9.3 0.2 MMAE 0.18 IV QW x 3 doses 50.4 (p>0.001) -2.3 8.6 5F9-vcMMAE 0.938 IV QW x 3 doses 20.7 (p>0.001) -2.3 3.8 202 WO 2011/050242 PCT/US2010/053686 5F9-vcMMAE 1.875 IV QW X 3 doses 41.3 (p>0.001) 5.6 6.6 5F9-vcMMAE 3.75 IV QW x 3 doses 65.7 (p>0.001) -2.2 16 5F9-vcMMAE 3.75 IV BIW x 6 doses 84.9 (py0,001) -1.8 31.9 5F9-vcMMAE 7.5 IV QW x 3 doses 84.3 (p>0.001) 6.5 (>47 days first tumor) 5F9-vcMMAE 10.0 IV QW x 3 doses 91.2 (p>0.001) 7.2 (>56 days first tumor) Pilot efficacy study with naked 5F9 anti hGCC antibody in CT26 hGCC/iuc #32 disseminated model (balb/c mice) [0483] This model tests the ability of naked antibodies to bind to GCC-expressing tumor cells in the circulation and prevent establishment of new tumors. Female balb/c mice were inoculated by i.v. with CT26 hGCC/luc #32 cells at I x105/mouse and 5x10 5 /mouse. Vehicle 0.9% NaC and non-specific antibody (naked 209) were administered to control groups for comparison to administration of naked 5F9. Both antibodies were engineered (in the pLKTOK58 vector) to have the IgG1 isotype, so their Fc regions could elicit an antibody-dependent cell-mediated cytotoxic response after binding to cell surface antigens (i.e., GCC for 5F9 and an unrelated target for 209). Dosing was started one day before inoculation by i.v, with a dosing schedule of once/week i.v x4 (q7d x 4). Tumor growth was monitored by Xenogen imaging system twice a week. Body weight and survival were monitored twice a week as well. Lung weight and images including MRI images were taken at the end of this study. [0484] As shown in Figure 2, both 5F9 groups (40mg/kg and 10mg/kg; lxlO5/mouse) show efficacy (on day 34p.i: T/C (treatment/control) is 0.04 to 0.05). T/C for the 5F9 group is 0.18 to 0.14 on day 34p.i compared to the 209 group. T/C for the 209 40mg/kg group is 0.64 compared to 0.9% NaCl (Normal Saline) group. No benefit was seen with 5F9 in 5x10 5 groups. [0485] Lung weight of each group at the end of this study was shown in Figure 3. T test: vehicle vs. 209 40mg/kg P=0.4; vehicle vs. 5F9 40mg/kg P<0.05; vehicle vs. 5F9 10mg/kg P <0.01. Visual inspection of lungs confirmed fewer tumor nodules in the 5F9 203 WO 2011/050242 PCT/US2010/053686 treated groups than in the vehicle or 209-treated groups. In vivo MRI of the mice showed massive lung tumor exfiltration to surrounding tissues and heart displacement in vehicle treated mice. In a 5F9 40 mg/kg-treated mouse, normal lung presentation was seen without evidence of tumor. [0486] A survival curve is shown in Figure 4. Significant increase in survival with 5F9 treated groups (1x10 5 ) was observed and there was no difference between 5F9 10 and 40 mg/kg groups. EXAMPLE 4: Generating an antibody production cell line [0487] To generate a stable CHO cell line clone expressing 5F9 with a productivity of>600mg/L, expression vectors for 5F9 were generated by subcloning light chain variable region (SEQ ID NO:19) and heavy chain variable region (SEQ ID NO:17) into the pLKTOK58 expression vector, containing WT human IgG1 Fec and the neomycin resistance gene. Expression of the 5F9 variable region-IgGI fusion product is under control of the EF-1 a promoter. Cloning and sequencing ofthe anti- GCC human monoclonal antibody 5F9 variable regions [04881 Total RNA was isolated (Qiagen's RNeasy kit) from human hybridoma 46.5F9 subclone 8.2. This hybridoma carries the "standard" published Kappa constant region of the light chain (GenBank accession # AW383625, or BM918539) and the "standard" published IgG2 constant region of the heavy chain (GenBank accession # BX640623, or AJ294731). 5' race-ready, poly-G tailed cDNA was synthesized by traditional methods (Nature Methods 2, 629-630 (2005)). The light chain variable region was PCR amplified from cDNA by 5' race using a poly-C anchor oligo in combination with a reverse primer specific for the Kappa constant region. The heavy chain variable region was amplified with a reverse primer specific for the IgG2 constant region in multiple combinations with forward primers specific to the known heavy chain leader sequences. PCR products were TOPO@ cloned (InvitrogenTM, Life Technologies, Inc.) and sequenced with M13F and M13R primers. 204 WO 2011/050242 PCTIUS2010/053686 Construction of mammalian expression vectors carrying anti-GCC human monoclonal antibody 5F9 [0489] Mammalian expression vectors carrying the 5F9 light and heavy variable regions were constructed to generate production CHO cell lines. For the native construct, the variable regions of the- 5F9 light and heavy chains were sub-cloned into pLKTOK58D (US Patent Application # 20040033561). This vector carries two mammalian selection markers: neomycin resistance and DHFR/methotrexate (for amplification). The vector allows co-expression of both light and heavy chains from tandem EFlalpha promoters, each located upstream of the vector's leader-Kappa constant and leader-IgGI (wild type Fc) constant regions. For sub-cloning, the variable regions of the light and heavy chains were PCR amplified from sequence-confirmed TOPO clones with gene-specific primers containing unique restriction sites for directional cloning into the junctions of the respective leader-Kappa and leader-IgGI regions of the vector. The sequences of the primers are as follows (5F9 variable region-specific sequences in bold fbnt): Native 5F9 light chain leader-variable primers: forward NotI 5'ataagaatGCGGCCGCCTCACCATGGGA TG GA GCTGTA TCA TCCTCTTCTTGGTAGCA CA GCTACAGGTGTCCA CTCCGAAATAGTGATGACGCAGTCTCCAGCCACCCTG-3' (SEQ ID NO: 234) reverse BsiWI 5'- GCCACCGTACGTTTGATTTCCACGTTGGTCCCTTGGCCGAACGTC-3' (SEQ ID NO:235 ) Native 5F9 heavy chain leader-variable primers forward EcoRI 5'ccgGAATTCCTCACCATGGGATGGAGCTGTATCATCCTCTTCTTGGTAGCAACAGCT ACAGGTGTCCACTCCCAGGTGCAGCTACAGCAGTGGGGCGCAGGAC-3' (SEQ ID NO:236) reverse Blpl 205 WO 2011/050242 PCT/US2010/053686 5'-GGAGGCTGAGCTGACGGTGACCAGGGTTCCCTGGCCCCAGTGGTC-3'(SEQ ID NO: 237) [0490] Clones were confirmed by double stranded DNA sequencing of both the light and heavy chains. [0491] Two transfection methods were used to introduce the constructs into CHO cells: the traditional MPI process and the Crucell process. CHO cell transfections were initiated with the native 5F9 construct using the traditional MPI process. Linearized and nonlinearized DNAs were used, with either electroporation or Lipopfectamine 2000 CD transfection. Approximately 30 stable pools were generated through selection in G418, non nucleoside medium and 5nM methotrexate. Based on FMAT analysis of antibody production levels, three stable pools were chosen for cloning. The pool with the highest production secreted antibody at 12.2ug/ml. These three pools have been frozen down. [04921 Crucell STAR elements can be evaluated to make 5F9 expression vectors containing a STAR element. 5F9/hIgGl heavy chain nucleotide sequence is: GAATTCCTCACCATGGGATGGAGCTGTATCATCCTCTTCTTGGTAGCAACAGCTACAGGTGTCC ACTCCCAGGTGCAGCTACAGCAGTGGGGCGCAGGACTGTTGAAGCCTTCGGAGACCCTGTCCCT CACCTGCGCTGTCTTTGGTGGGTCTTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCCCCCAG GGAAGGGGCTGGAGTGGATTGGGGAAATCAATCATCGTGGAAACACCAACGACAACCCGTCCC TCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCGCCCTGAAGCTGAGTTC TGTGACCGCCGCGGACACGGCTGTTTATTACTGTGCGAGAGAACGTGGATACACCTATGGTAAC TTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCAGCTCAGCCTCCACCAAGGGCCCATCGG TCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGT CAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGT GCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTG CCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACC AAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCA GCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCA TGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGG TCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG AGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAA TGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCAT CTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGA GCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCC 206 WO 2011/050242 PCT/US2010/053686 GTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGAC TCCGACGGCTCCfTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGG AACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCT CCCTGTCTCCGGGTAAATAATAGGGATAACAGGGTAATACTAGAG (SEQ ID NO:230) 5F9/hIgGl heavy chain protein sequence is; MGWSCIILFLVATATGVHSQVQLQQWGAGLLKPSETLSLTCAVFGGSFSGYYWSWIRQPPGKGLE WIGEINHRGNTNDNPSLKSRVTISVDTSKNQFALKLSSVTAADTAVYYCARERGYTYGNFDHWGQ GTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQS SGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFP PKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVL HQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPS DIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKS LSLSPGK (SEQ ID NO:23 1) 5F9/hKappa light chain nucleotide sequence is: GCGGCCGCCTCACCATGGGATGGAGCTGTATCATCCTCTTCTTGGTAGCAACAGCTACAGGTGT CCACTCCGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCC ACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGAAACTTAGCCTGGTATCAGCAGAAACCTG GCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGAATCCCAGCCAGGTT CAGTCGCAGTGGGTCTGGGACAGAGTTCACTCCACCATCGGCAGCCTGCAGTCTGAAGATTTT GCAGTTTATTACTGTCAGCAGTATAAAACCTGGCCrCGGACGTTCGGCCAAGGGACCAACGTGG AAATCAAACGTACGGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAG TGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGC AAGGACAGCACCTACAGCCTCAGCAGCACCCTGACCCTGAGCAAAGCAGACTACGAGAAACAC AAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAAC AGGGGAGAGTGTTAGTCTAGA (SEQ ID NO:232) 207 WO 2011/050242 PCT/US2010/053686 5F9/hKappa light chain protein sequence is: MGWSCIILFLVATATGVHSEIVMTQSPATLSVSPGERATLSCRASQSVSRNLAWYQQKPGQAPRLLI YGASTRATGIPARFSGSGSGTEFTLTIGSLQSEDFAVYYCQQYKTWPRTFGQGTNVEIKRTVAAPSVF IFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSK ADYEKHKVYACEVTHQGLSSPVTKSFNRGEC (SEQ ID NO:233) [0493] The heavy and light chain nucleic acid sequences for 5F9 listed below were inserted into pTOK58D vector: atgggatggagctgtatcatcctcttcttggtagcaacagctacaggtgtccactccgaaatagtgatgacgeagtetccagccaccctgtctgt ctccagg ggaaagagccaccctctcctgcagggccagtcagagtgttagcagaaaettagcctggtatcagcagaaacctggccagg ctcccaggctcctcatctatggtgcatecaccagggccactggaatcccagccaggttcagtggcagtgggtetgggaca gagttcactctcaccatcggcagcctgcagtctgaagattttgcagtttattactgtcagcagtataaaacctggcctcg gacgttcggccaagggaccaacgtggaaatcaaacgtacggtggctgcaccatctgtcttcatcttcccgccatctgatg agcagttgaaatctggaactgcctctgttgtgtgcctgtgaataacttctatccagagaggccaaagtacagtggaag gtggataacgccctccaatcgggtaactcccaggagagtgtcacagagcaggacagcaaggacagcacctacagcctcag cagcaccctgaccctgagcaaagcagactacgagaaacacaaagtctacgcctgcgaagtcacccatcagggcctgaget cgcccgtcacaaagagottcaacaggggagagtgttag (SEQ ID NO:308) atgggatggagctgtatcatcctcttcttggtagcaacagct acaggtgtccactcccaggtgcagctacagcagtggggcgcaggactgttgaagccttcggagaccctgtecctcacctg cgctgtctttggtgggtctttcagtggttactactggagctggatccgccagcccccagggaaggggctggagtggattg gggaaatcaatcatcgtggaaacaccaacgacaacccgtccctcaagagtcgagtcaccatatcagtagacacgtecaag aaccagttcgccctgaagctgagttctgtgaccgccgcggacacggctgtttattactgtgcgagagaacgtggatacac ctatggtaaetttgaccactggggccagggaaccctggtcaccgtcagctcagcctccaccaagggcccatcggtttcc 208 WO 2011/050242 PCT/(JS2010/053686 ccctggcaccetcctocaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaacog gtgacggtgtcgtggaactcaggcgccctgaccagcggcgtgcacaccttccggctgtctacagtoctcaggactota ctccetcagcagcgtggtgaccgtgccetccagcagcttgggcacccagacetacatctgcaacgtgaatacaagccca gcaacaccaaggtggacaagaaagttgagcccaaatcttgtgacaaaactcacacatgcccaccgtgcccagcacctgaa ctcctggggggaccgtcagtcttcctcttccccccaaaacccaaggacaccctcatgatetcccggacccctgaggtcac atgcgtggtggtggacgtgagccacgaagacoctgaggtcaagttcaactggtacgtggacggcgtggaggtgcataatg ccaagacaaagccgcgggaggagcagtacaacagcacgtaccgtgtggtcagcgtoctcaccgtoctgcaccaggactgg ctgaatggcaaggagtacaagtgcaaggtctocaacaaagccctceccagcccccatcgagaaaaccatctccaaagccaa agggcagccccgagaaccacaggtgtacaccctgcccccatccogggatgagctgaccaagaaccaggtcagcctgacct goctggtcaaaggcttctatcccagcgacatcgccgtggagtgggagagcaatgggcagccggagaacaactacaagaco acgcctcccgtgctggactccgacggctccttcttcotctacagcaagctcaccgtggacaagagcaggtggcagcaggg gaacgtcttctcatgctccgtgatgcatgaggctctgcacaaccactacacgcagaagagcctctccctgtetccgggta aataa (SEQ ID NO:309) [0494] The sequences encoding the Abx-229 heavy and light chains sequence below were inserted into pTOK58D vector: atggagtttgggctgagctggctttttcttgtggetatt ttaaaaggtgtocagtgtgaggtgcagctgttggagtctgggggaggcttggtacagcctggggggtccctgagactctc ctgtgcagcctctggattcacctttagccgctatgccatgaactgggtccgccaggctccagggaaggggctggagtggg totcaggtattagtgggagtggtggtaggacatactacgcagactccgtgaagggccggttcaccatctccagagacaat tccaagaacacactatatctgcaaatgaacagcctgagagccgaggacacggccgtatattactgtgegaaagatcgcga tttttggagtggtccatttgactactggggccagggaaccctggtcaccgtcagetcagcctccaccaagggcccatcgg tcttccccctggcaccctcetccaagagcacotctgggggcacagcggccctgggctgcctggtcaaggactacttcccc gaaccggtgacggtgtcgtggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctcagg actctactccetcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaacgtgaatcaca agcccagcaacaccaaggtggacaagaaagttgagcccaaatcttgtgacaaaactcacacatgcccaccgtgcccagoa 209 WO 2011/050242 PCT/US2010053686 cctgaactcotggggggaccgtcagtcttcctcttccccccaaaacccaaggacaccctcatgatctcccggacccctga ggtcacatgcgtggtggtggacgtgagccacgaagaccctgaggtcaagttcaactggtacgtggacggcgtggaggtgc ataatgccaagacaaagccgcgggaggagcagtacaacagcacgtaccgtgtggtcaggtcctcaccgtctgaccag gactggctgaatggcaaggagtacaagtgcaaggtctccaacaaagccctcccagcccccatgagaaaaccatctccaa agccaaagggcageccgagaaccacaggtgtacaccctgcccccatcccgggatgagtgaccaagaaccaggtcagcc tgacctgcctggtcaaaggcttctatcccagcgacatcgccgtggagtgggagagcaatgggcagccggagaacaactac aagaccacgcctcccgtgctggactccgacggctccttcttcctctacagcaagctaccgtggacaagagaggtggca gcaggggaacgtcttctcatgctccgtgatgcatgaggctctgacaaccactacacgcagaagagcctctcectgttc cgggtaaataa (SEQ ID NO:310) atgaggctccctgctcagcttctc ttcctcctgctactctggctcccagataccactggagaaatagtgatgacgccgtcttcagccaccctgtctgtgtctcc aggggagagagccaccctctcctgcagggccagtcagagtgttagtagaaaettagcctggtaccagcagaaacctggcc aggctcccaggctcctcatctatggtgcatccaccagggccactggtatcccagccaggttcagtggcagtgggtctggg acagaattcactctcaccatcageagcctgcagtctgaagattttgcagtttattactgtcaccagtatagtaactggat gtgcagttttggccaggggaccaagctggagatcaaacgtacggtggctgcaccatctgtcttcatcttcccgccatctg atgagcagttgaaatctggaactgcctctgttgtgtgcctgctgaataacttctatcccagagaggccaaagtacagtgg aaggtggataacgccctccaatcgggtaactcccaggagagtgtcacagagcaggacagcaaggacagcacctacagcct cagcagcaccctgaccctgagcaaagcagactacgagaaacacaaagtctacgcctgcgaagtcacccatcagggcctga gctcgcccgtcacaaagagcttcaacaggggagagtgttag (SEQ ID NO:31 1) [04951 While this invention has been shown and described with references to provided embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention encompassed by the appended claims. 210 Throughout this specification, unless the context requires otherwise, the word "comprise" or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element or integer or method step or group of elements or integers or method steps but not the exclusion of any element or integer or method step or group of elements or integers or method steps. Reference to any prior art in this specification is not, and should not be taken as, an acknowledgment or any form of suggestion that this prior art forms part of the common general knowledge in any country. 21 Oa
Claims (46)
1. A method of producing an immunoconjugate, the method comprising: providing an anti-GCC antibody molecule and contacting the antibody molecule with a non-antibody moiety comprising a therapeutic agent or label under conditions that allow the production of an immunoconjugate, thereby producing the immunoconjugate, wherein the antibody molecule comprises a light chain variable region and a heavy chain variable region, wherein the light chain variable region comprises three light chain complementarity determining regions (LCDR1, LCDR2, and LCDR3), and the heavy chain variable region comprises three heavy chain complementarity determining regions (HCDR1, HCDR2, and HCDR3), wherein the light chain variable region comprises: an LCDR1 comprising the amino acid sequence of SEQ ID NO: 112, an LCDR2 comprising the amino acid sequence of SEQ ID NO: 114 and an LCDR3 comprising the amino acid sequence of SEQ ID NO:116; and wherein the heavy chain variable region comprises: an HCDR1 comprising the amino acid sequence of SEQ ID NO: 106, an HCDR2 comprising the amino acid sequence of SEQ ID NO: 108 and an HCDR3 comprising the amino acid sequence of SEQ ID NO:110.
2. The method of Claim 1, wherein the antibody molecule further comprises human or human derived light and heavy variable region frameworks, and/or wherein said anti-GCC antibody is an IgGI antibody.
3. The method of Claim 1 or 2, wherein the light chain variable region comprises the amino acid sequence of SEQ ID NO:20 and the heavy chain variable region comprises the amino acid sequence of SEQ ID NO: 18. 211 H: \aar\lnterwoven\NRPortbl\DCC\AAR\7379956_1.docx-19/01/2015
4. The method of any of Claims 1 to 3, wherein the immunoconjugate is characterized by formula (1): Ab X- Z or a pharmaceutically acceptable salt thereof, wherein: Ab is the anti-GCC antibody molecule; X is a linker moiety which connects Ab and Z; Z is a therapeutic agent or a label; and m is the number of -X-Z moieties per anti-GCC antibody molecule in the immunoconjugate of formula (I), and m is an integer from about I to about 15.
5. The method of Claim 4, wherein the linker -X- has the formula -Aa-Ww-Yy-, and the immunoconjugate is characterized by formula (II): Ab-#A-W-Y or a pharmaceutically acceptable salt thereof, wherein: -A- is a Stretcher unit; a is 0 or 1; each -W- independently is an Amino Acid unit; w is an integer ranging from 0 to 12; -Y- is a self-immolative Spacer unit; y is 0, 1, or 2; Z is a therapeutic agent or label; and m is an integer from about I to about 15.
6. The method of Claim 4, wherein Z is a detectable label or a therapeutic radiolabel. 212 H: \aar\lnterwoven\NRPortbl\DCC\AAR\7379956_1.docx-19/01/2015
7. The method of Claim 6, wherein the therapeutic radiolabel comprises an a emitter.
8. The method of Claim 7, wherein the a-emitter is 213Bi or mAc.
9. The method of Claim 6, wherein the therapeutic radiolabel comprises a p emitter.
10. The method of Claim 9, wherein the j-emitter is 90Y or 77Lu.
11. The method of Claim 4 or 5, wherein Z is a cytostatic or cytotoxic agent.
12. The method of Claim 11, wherein the cytostatic or cytotoxic agent is selected from antimetabolites; alkylating agents; anthracyclines; antibiotics; antimitotic agents; topoisomerase inhibitors; or proteasome inhibitors.
13. The method of Claim 12, wherein the antimetabolite is selected from azathioprine, 6-mercaptopurine, 6-thioguanine, fludarabine, pentostatin, cladribine, 5 fluorouracil (5FU), floxuridine (FUDR), cytosine arabinoside (cytarabine), methotrexate, trimethoprim, pyrimethamine, or pemetrexed.
14. The method of Claim 12, wherein the alkylating agent is selected from cyclophosphamide, mechlorethamine, uramustine, melphalan, chlorambucil, thiotepa/chlorambucil, ifosfamide, carmustine, lomustine, streptozocin, busulfan, dibromomannitol, cisplatin, carboplatin, nedaplatin, oxaliplatin, satraplatin, triplatin tetranitrate, procarbazine, altretamine, dacarbazine, mitozolomide, or temozolomide.
15. The method of Claim 12, wherein the anthracycline is selected from daunorubicin, doxorubicin, epirubicin, idarubicin, or valrubicin. 213 H: \aar\lnterwoven\NRPortbl\DCC\AAR\7379956_1.docx-19/01/2015
16. The method of Claim 12, wherein the antibiotic is selected from dactinomycin, bleomycin, mithramycin, anthramycin, streptozotocin, gramicidin D, mitomycins, duocarmycins, or calicheamicins.
17. The method of Claim 16, wherein the mitomycin is mitomycin C.
18. The method of Claim 16, wherein the duocarmycin is CC-1065.
19. The method of Claim 12, wherein the antimitotic agent is selected from maytansinoids, auristatins, dolastatins, cryptophycins, vinca alkaloids, taxanes, or colchicines.
20. The method of Claim 19, wherein the vinca alkaloid is selected from vincristine, vinblastine, vindesine, or vinorelbine.
21. The method of Claim 19, wherein the taxane is paclitaxel or docetaxel.
22. The method of Claim 12, wherein the topoisomerase inhibitor is selected from irinotecan, topotecan, amsacrine, etoposide, teniposide, or mitoxantrone.
23. The method of Claim 12, wherein the proteasome inhibitors is a peptidyl boronic acid.
24. The method of Claim 4 or 5, wherein Z is a maytansinoid or a maytansine.
25. The method of Claim 24, wherein the maytansinoid or maytansine is N deacetyl-N 2 -(4-mercapto-1-oxopentyl)maytansine (DM3), N 2 -deacetyl-N 2 -(3-mercapto-1 oxopropyl)-maytansine (DM1), or N 2 -deacetyl-N 2 -(4-mercapto-4-methyl-1 oxopentyl)maytansine (DM4). 214 H: \aar\lnterwoven\NRPortbl\DCC\AAR\7379956_1.docx-19/01/2015
26. The method of Claim 24 or 25, wherein the immunoconjugate has formula (I 1): 00 N N A - S 4 N - CI \ 00LK~f MeO N 0 HN I~. Ab 0 N Ok-0 MeO HO H " (I-1) or a pharmaceutically acceptable salt thereof.
27. The method of Claim 24 or 25, wherein the immunoconjugate has formula (I 2): O O Ab Ci \N S MeO N 0 N O MeO HO H or a pharmaceutically acceptable salt thereof. 215 H: \aar\lnterwoven\NRPortbl\DCC\AAR\7379956_1.docx-19/01/2015
28. The method of Claim 24 or 25, wherein the immunoconjugate has formula (I 3): 0 CI \N0 Ab MeO N 0 0 N O" MeO HO H m (I-3) or a pharmaceutically acceptable salt thereof.
29. The method of any of Claims 24 to 28, wherein m is an integer from about I to about 10.
30. The method of any of Claims 24 to 29, wherein m is an integer from about 3 to about 7.
31. The method of any of Claims 24 to 30, wherein m is an integer from about 3 to about 5.
32. The method of any of Claims 24 to 31, wherein m is about 4.
33. The method of Claim 4 or 5, wherein Z is a dolastatin or auristatin. 216 H: \aar\lnterwoven\NRPortbl\DCC\AAR\7379956_1.docx-19/01/2015
34. The method of Claim 33, wherein Z is an auristatin moiety of formula (X-A) or formula (X-B): R 3 0 R 7 H R R 20 NN 'N N (-A 2 R e R19 (X-A) R 2 0 R 4 R 5 6 R 8 0 R 8 0 R 21 R 3 0 R 7 H R 9 0 N N R11 R 2 0 R 4 R 5 R6 R 8 0 G R 8 0 (X-B)
35. The method of Claim 33 or 34, wherein Z is selected from compounds of formulae (XIII)-(XXIII): H 3 C CH 3 H 3 C 0 CH 3 HO H CH 3 HN N N N CH 3 CH 3 0 CH 3 OCH 3 O H H 3 C CH 3 OCH 3 0 (XIII) NH 2 H O NN,,'' N N N N 0 OCH 3 O H OCH 3 0 (XIV) 217 H: \aar\lnterwoven\NRPortbl\DCC\AAR\7379956_1docx-19/01/2015 H HN N C 3 OC C 3 O OC H 3 H CH3 (X 3 (XV) H 3 C CH 3 H 3 C H 0 CH 3 H N NS CH' 3 0 u CH OCH 3 H O CH 3 OCH 3 0 N CH 3 (X) 0 H 2 N H N " N N 0 C30OCH 3 0 (XVII) H 3 C CH 3 H 3 C H 0C3CH 3 0 -~NHI H3CNI N,, H N N N N,,, N CH 3 0H3C CH 3 CH3 O CH 3 O H -~ (X VIMI H 3 C CH 3 H 3 C H 0 CH 3 H NN CH' 3 0 CH 3 OCHU 3 0H H 3 C CH 3 OCH 3 0 N (XIX) 218 H: \aar\lnterwoven\NRPortbl\DCC\AAR\7379956_1docx-19/01/2015 H N NH 2 N N NN NO N N 0OCHOH3O(ff 4 N 0 OCH 3 00 M) 0 H N ,,H HN ' N N N A E V B A F P M M A E or M A F 0 0 0n 1- 0 OH(X) 0 0 0 H NO0 N N NH 0 OCH 3 0 HC3 0M I 0 __ 0 0 0 H N,,," HN N N Y N 0 0CH 3 H 0 H M I
36. The method of any of Claims 33 to 35, wherein Z is selected from AEB, AEVB, AFP, MIMAE, or MMAF. 219 H: \aar\lnterwoven\NRPortbl\DCC\AAR\7379956_1.docx-19/01/2015
37. The method of any of Claims 33 to 36, wherein the immunoconjugate has formula (1-4): or a pharmaceutically acceptable salt thereof.
38. The method of any of Claims 33 to 36, wherein the immunoconjugate has formula (1-5): or a pharmaceutically acceptable salt thereof.
39. The method of any of Claims 33 to 36, wherein the immunoconjugate has formula (1-6): Ab- v VN-' yN NZ m (1-6) or a pharmaceutically acceptable salt thereof. 220 H: \aar\lnterwoven\NRPortbl\DCC\AAR\7379956_1.docx-19/01/2015
40. The method of any of Claims 33 to 36, wherein the immunoconjugate has formula (1-7): Ab, or a pharmaceutically acceptable salt thereof.
41. The method of any one of Claims 33 to 40, wherein m is from about I to about 5.
42. The method of any one of Claims 33 to 40, wherein m is from about 2 to about 10.
43. The method of any one of Claims 33 to 42, wherein m is about 4.
44. An immunoconjugate produced by the method of any of Claims 1 to 43.
45. A pharmaceutical composition comprising the immunoconjugate of Claim 44 and a pharmaceutically acceptable carrier.
46. A method according to any one of Claims 1 to 43 or an immunoconjugate according to Claim 44 or a pharmaceutical composition according to Claim 45 substantially as herein described with reference to the Figures and/or Examples. 221
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NANDI, A., et al., "Epitope Conservation and Immunohistochemical Localization of the Guanylin/Stable Toxin Peptide Receptor, Guanylyl Cyclase C," Journal of Cellular Biochemistry, 1997, Vol. 66, pages 500-511 * |
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