AU2013201617B2 - 4-1BB binding molecules - Google Patents

Abstract

The present disclosure provides isolated binding molecules that bind to human 4-1BB, nucleic acid molecules encoding an amino acid sequence of the binding molecules, vectors comprising the nucleic acid molecules, host cells containing the vectors, methods of making 5 the binding molecules, pharmaceutical compositions containing the binding molecules, and methods of using the binding molecules or compositions.

Description

- 1 4-1BB BINDING MOLECULES The present application is a divisional application of Australian Application No. 2011300445, which is incorporated in its entirety herein by reference. FIELD OF THE INVENTION The present disclosure relates to antibodies, and particularly antibodies that bind to human 4-1 BB. BACKGROUND Any discussion of the prior art throughout the specification should in no way be considered as an admission that such prior art is widely known or forms part of common general knowledge in the field. 4-1 BB (also referred to as CD1 37, TNFRSF9, etc) is a transmembrane protein of the Tumor Necrosis Factor receptor superfamily (TNFRS). Current understanding of 4-1 BB indicates that expression is generally activation dependent and is present in a broad subset of immune cells including activated NK and NKT cells, regulatory T cells, dendritic cells (DC), stimulated mast cells, differentiating myeloid cells, monocytes, neutrophils, and eosinophils (Wang, 2009, Immunological Reviews 229: 192-215). 4-1BB expression has also been demonstrated on tumor vasculature (Broll, 2001, Amer. J Clin. Pathol. 115(4):543-549; Seaman, 2007, Cancer Cell 11: 539-554) and at sites of inflamed or atherosclerotic endothelium (Drenkard, 2007 FASEB J. 21: 456-463; Olofsson, 2008, Circulation 117: 1292 1301). The ligand that stimulates 4-1 BB, i.e., 4-1 BB Ligand (4-1 BBL), is expressed on activated antigen-presenting cells (APCs), myeloid progenitor cells, and hematopoietic stem cells. Human 4-1 BB is a 255 amino acid protein (Accession No. NM_001561; NP_001552). The complete human 4-1 BB amino acid sequence is provided in SEQ ID NO:68. The protein comprises a signal sequence (amino acid residues 1-17), followed by an extracellular domain (169 amino acids), a transmembrane region (27 amino acids), and an intracellular domain (42 amino acids) (Cheuk ATC et al. 2004 Cancer Gene Therapy 11: 215-226). The receptor is expressed on the cell surface in monomer and dimer forms and likely trimerizes with 4-1 BB ligand to signal. Numerous studies of murine and human T cells indicate that 4-1BB promotes enhanced cellular proliferation, survival, and cytokine production (Croft, 2009, Nat Rev Immunol 9:271-285). Studies have indicated that some 4-1BB agonist mAbs increase costimulatory molecule expression and markedly enhance cytolytic T lymphocyte responses, resulting in anti-tumor efficacy in various models. 4-1BB agonist mAbs have demonstrated efficacy in prophylactic and therapeutic settings. Further, 4-1 BB monotherapy and combination -2 therapy tumor models have established durable anti-tumor protective T cell memory responses (Lynch, 2008, Immunol Rev. 22: 277-286). 4-1BB agonists also have been shown to inhibit autoimmune reactions in a variety of art-recognized autoimmunity models (Vinay, 2006, J Mol Med 84:726-736). This dual activity of 4-1 BB offers the potential to provide anti-tumor activity while dampening autoimmune side effects that can be associated with immunotherapy approaches that break immune tolerance. There is a long-felt unmet need for antibodies that bind human 4-1 BB, increase a 4-1 BB-mediated response, and thereby provide a potential therapeutic for treatment of various diseases and conditions, including cancer. It is an object of the present invention to overcome or ameliorate at least one of the disadvantages of the prior art, or to provide a useful alternative. SUMMARY The disclosure relates to an isolated binding molecule that binds to human 4-1 BB, such as an antibody or a binding fragment thereof, or derivative thereof. The disclosure also relates to a composition comprising a binding molecule that binds to 4-1 BB. Further, the present disclosure relates to methods for treating a disease and/or condition associated with or mediated by 4-1 BB signaling by using one or more binding molecules of the disclosure. In some aspects, the disclosure provides isolated antibodies that bind to human 4-1 BB. According to a first aspect, the present invention provides an isolated antibody or antigen-binding portion thereof wherein said antibody or antigen-binding portion binds human 4-1BB extracellular domain at an epitope located within amino acid residues 115-156 of SEQ ID NO: 68. According to a second aspect, the present invention provides a pharmaceutical composition comprising an antibody or antigen-binding portion thereof according to the first aspect and a pharmaceutically acceptable carrier. According to a third aspect, the present invention provides a method of treating abnormal cell growth in a subject in need thereof, comprising administering to said subject an effective amount of an antibody or antigen-binding portion thereof according to the first aspect or the pharmaceutical composition according to the second aspect. According to a fourth aspect, the present invention provides a method of reducing tumor cell metastasis in a subject in need thereof, comprising administering to said subject an effective amount of an antibody or antigen-binding portion thereof according to the first aspect or the pharmaceutical composition according to the second aspect. According to a fifth aspect, the present invention provides a method of treating a disorder in a subject in need thereof, comprising administering to said subject a therapeutically - 2a effective amount of an antibody or antigen-binding portion thereof according to the first aspect or the pharmaceutical composition according to the second aspect. According to a sixth aspect, the present invention provides a combination therapy comprising an antibody or antigen-binding portion thereof according to the first aspect and a pharmaceutically acceptable carrier in combination with an immunotherapeutic agent. According to a seventh aspect, the present invention provides use of an antibody or antigen-binding portion thereof according to the first aspect in the preparation of a medicament for: reducing tumor growth in a subject; reducing tumor cell metastasis in a subject; or treating a disorder in a subject. According to an eighth aspect, the present invention provides an isolated antibody or an antigen-binding portion thereof wherein said antibody or antigen-binding portion binds human 4-1 BB extracellular domain, and wherein said binding is abrogated by one or more mutations of SEQ ID NO: 68 selected from K1 15Q, C121 R, R1 34Q, R1 54S, or V1 56A. According to a ninth aspect, the present invention provides a pharmaceutical composition comprising an antibody or antigen-binding portion thereof according to the eighth aspect and a pharmaceutically acceptable carrier. According to a tenth aspect, the present invention provides a method of reducing tumor growth in a subject in need thereof, comprising administering to the subject an effective amount of an antibody or antigen-binding portion thereof according to the eighth aspect or the pharmaceutical composition according to the ninth aspect. According to an eleventh aspect, the present invention provides a method of treating cancer in a subject in need thereof, comprising administering to the subject an effective amount of an antibody or antigen-binding portion thereof according to the eighth aspect or the pharmaceutical composition according to the ninth aspect. According to a twelfth aspect, the present invention provides use of an antibody or antigen-binding portion thereof according to the eighth aspect in the preparation of a medicament for: reducing tumor growth in a subject; or treating cancer in a subject. In one particular aspect, the isolated antibody binds human 4-1 BB at an epitope comprising amino acid residues 115 - 156 of SEQ ID NO: 68. In some particular embodiments, the antibody comprises the H-CDR1 amino acid sequence of SEQ ID NO: 29, H-CDR2 amino acid sequence of SEQ ID NO: 30, and H-CDR3 amino acid sequence of SEQ ID NO: 31. In other particular embodiments, the antibody comprises the L-CDR1 amino acid - 2b sequence of SEQ ID NO: 34, L-CDR2 amino acid sequence of SEQ ID NO: 35, and L-CDR3 amino acid sequence of SEQ ID NO: 36. In another particular aspect, the isolated antibody binds human 4-1 BB with a KD of 600 nM or less, 100 nM or less, 50 nM or less, 10 nM or less, 5 nM or less, or 1 nM or less, for the human 4-1 BB extracellular domain as measured with the BIACore assay described in this disclosure. In another particular aspect, the isolated antibody comprises: (a) an H-CDR1 as set forth in SEQ ID NO:1 , SEQ ID NO:1 5, or SEQ ID NO:29; (b) an H-CDR2 as set forth in SEQ ID NO:2, SEQ ID NO:1 6, or SEQ ID NO:30; and (c) an H-CDR3 as set forth in SEQ ID NO:3, SEQ ID NO: 17, or SEQ ID NO:31. In another particular aspect, the isolated antibody comprises: (a) an L-CDR1 as set forth in SEQ ID NO:6, SEQ ID NO: 20, or SEQ ID NO:34; (b) an L-CDR2 as set forth 3 in SEQ ID NO:7, SEQ ID NO:21, or SEQ ID NO:35; and (c) an L-CDR3 as set forth in SEQ ID NO:8, SEQ ID NO:22, SEQ ID NO:36, or SEQ ID NO: 55. In a further aspect, the isolated antibody comprises: (a) an H-CDR1 as set forth in SEQ ID NO:1 , SEQ ID NO:15, or SEQ ID NO:29; (b) an H-CDR2 as set forth in SEQ 5 ID NO:2, SEQ ID NO:16, or SEQ ID NO:30; and (c) an H-CDR3 as set forth in SEQ ID NO:3, SEQ ID NO: 17, or SEQ ID NO:31; and further comprises: (d) an L-CDR1 as set forth in SEQ ID NO:6, SEQ ID NO: 20, or SEQ ID NO:34; (e) an L-CDR2 as set forth in SEQ ID NO:7, SEQ ID NO:21, or SEQ ID NO:35; and (f) an L-CDR3 as set forth in SEQ ID NO:8, SEQ ID NO:22, SEQ ID NO:36, or SEQ ID NO: 55. 10 In some further particular aspects, the isolated antibody, is selected from the group consisting of: (a) an antibody or antigen-binding portion thereof, comprising: an H-CDR1 as set forth in SEQ ID NO:1, an H-CDR2 as set forth in SEQ ID NO:2, and an H-CDR3 as set forth in SEQ ID NO:3; 15 (b) an antibody or antigen-binding portion thereof, comprising an H-CDR1 as set forth in SEQ ID NO:15, an H-CDR2 as set forth in SEQ ID NO:16, and an H-CDR3 as set forth in SEQ ID NO:17, and (c) an antibody or antigen-binding portion thereof, comprising an H-CDR1 as set forth in SEQ ID NO:29, an H-CDR2 as set forth in SEQ ID NO:30, and an H-CDR3 as 20 set forth in SEQ ID NO:31. In some further aspects, the disclosure provides an isolated antibody, or antigen binding portion thereof, that specifically binds human 4-11BB, wherein said antibody or antigen-binding portion is selected from the group consisting of: (a) an antibody or antigen-binding portion thereof, comprising an L-CDR1 as set 25 forth in SEQ ID NO:6, an L-CDR2 as set forth in SEQ ID NO:7, and an L-CDR3 as set forth in SEQ ID NO:8. (b) an antibody or antigen-binding portion thereof, comprising an L-CDR1 as set forth in SEQ ID NO:20, an L-CDR2 as set forth in SEQ ID NO:21, and an L-CDR3 as set forth in SEQ ID NO:22. 30 (c) an antibody or antigen-binding portion thereof, comprising an L-CDR1 as set forth in SEQ ID NO:34, an L-CDR2 as set forth in SEQ ID NO:35, and an L-CDR3 as set forth in SEQ ID NO:36; and 4 (d) an antibody or antigen-binding portion thereof, comprising an L-CDR1 as set forth in SEQ ID NO:34, an L-CDR2 as set forth in SEQ ID NO:35, and an L-CDR3 as set forth in SEQ ID NO:55. In some further particular aspects, the isolated antibody is selected from the 5 group consisting of: (a) an antibody or antigen-binding portion thereof, comprising: an H-CDR1 as set forth in SEQ ID NO:1, an H-CDR2 as setforth in SEQ ID NO:2, an H-CDR3 as set forth in SEQ ID NO:3; an L-CDRI as set forth in SEQ ID NO:6, an L-CDR2 as set forth in SEQ ID NO:7, and an L-CDR3 as set forth in SEQ ID NO:8; 10 (b) an antibody or antigen-binding portion thereof, comprising an H-CDR1 as set forth in SEQ ID NO:15, an H-CDR2 as set forth in SEQ ID NO:16, an H-CDR3 as set forth in SEQ ID NO:17; an L-CDR1 as set forth in SEQ ID NO:20, an L-CDR2 as set forth in SEQ ID NO:21, and an L-CDR3 as set forth in SEQ ID NO:22. (c) an antibody or antigen-binding portion thereof, comprising an H-CDR1 as set 15 forth in SEQ ID NO:29, an H-CDR2 as set forth in SEQ ID NO:30, an H-CDR3 as set forth in SEQ ID NO:31; an L-CDR1 as set forth in SEQ ID NO:34, an L-CDR2 as set forth in SEQ ID NO:35, and an L-CDR3 as set forth in SEQ ID NO:36; and (d) an antibody or antigen-binding portion thereof, comprising an H-CDR1 as set forth in SEQ ID NO:29, an H-CDR2 as set forth in SEQ ID NO:30, an H-CDR3 as set 20 forth in SEQ ID NO:31; an L-CDR1 as set forth in SEQ ID NO:34, an L-CDR2 as set forth in SEQ ID NO:35, and an L-CDR3 as set forth in SEQ ID NO:55. In a further particular aspect, the isolated antibody comprises a VH chain amino acid sequence as set forth in SEQ ID NO:4, SEQ ID NO:18, SEQ ID NO:32, and SEQ ID NO:43. 25 In a further particular aspect, the isolated antibody comprises a VL chain amino acid sequence as set forth in SEQ ID NO:9, SEQ ID NO:23, SEQ ID NO:37, SEQ ID NO:45, SEQ ID NO:51, SEQ ID NO:56, SEQ ID NO:60, or SEQ ID NO:64. In a further particular aspect, the isolated antibody comprises a VH domain amino acid sequence as set forth in any one of SEQ ID NOs:4, 18, 32, and :43, and further 30 comprises a VL domain amino acid sequence as set forth in any one of SEQ ID NOs:9, SEQ ID NO: 23, SEQ ID NO: 37, SEQ ID NO: 45, SEQ ID NO: 51, SEQ ID NO: 56, SEQ ID NO: 60, and SEQ ID NO: 64. In a further particular aspect, the isolated antibody is selected from the group consisting of: 5 (a) an antibody comprising a VH chain amino acid sequence as set forth in SEQ ID NO:4 and a V 2 chain amino acid sequence as set forth in SEQ ID NO:9; (b ) an antibody comprising a VH chain amino acid sequence as set forth in SEQ ID NO:18 and a VL chain amino acid sequence as set forth in SEQ ID NO:23; 5 (c) an antibody comprising a VH chain amino acid sequence as set forth in SEQ ID NO:32 and a VL chain amino acid sequence as set forth in SEQ ID NO:37 or SEQ ID NO 56; and (d) an antibody comprising a VH chain amino acid sequence as set forth in SEQ ID NO:43 and a VL chain amino acid sequence as set forth in SEQ ID NO:45, SEQ ID 10 NO:51, SEQ ID NO: 60, or SEQ ID NO: 64. In a still further particular aspect, the isolated antibody provided by the present disclosure comprises a VH chain that is encoded by (i) a nucleic acid sequence comprising SEQ ID NO:11, SEQ ID NO:25, SEQ ID NO:39, SEQ ID NO:47, or (ii) a nucleic acid sequences that hybridizes under high stringency conditions to the 15 complementary strand of SEQ ID NO:11, SEQ ID NO:25, SEQ ID NO:39, or SEQ ID NO:47. In a still further particular aspect, the isolated antibody comprises a VL chain that is encoded by (i) a nucleic acid sequence comprising SEQ ID NO:12, SEQ ID NO:26, SEQ ID NOA0, SEQ ID NO:48, SEQ ID NO:53, SEQ ID NO:58, SEQ ID NO:62, or SEQ 20 ID NO:66, or (ii) a nucleic acid sequences that hybridizes under high stringency conditions to the complementary strand of SEQ ID NO:12, SEQ ID NO:26, SEQ ID NO:40, SEQ ID NO:48, SEQ ID NO:53, SEQ ID NO:58, SEQ ID NO:62, or SEQ ID NO:66. In a further particular aspect, there is provided an isolated antibody that 25 competes, and/or cross-competes for binding to human 4-11B with an illustrative antibody selected from MOR-6032, MOR-7361, MOR-7480, MOR-7480.1, MOR-7480.2, MOR 7483, MOR-7483.1, or MOR-7483.2. In a further particular aspect, there is provided an isolated antibody that binds to the same epitope on human 4-1BB as any of the antibodies disclosed herein. In some 30 embodiments, the disclosure provides an isolated antibody that binds to the same epitope on human 4-1BB as an illustrative antibody selected from MOR-6032, MOR 7361, MOR-7480, MOR-7480.1, MOR-7480.2, MOR 7483, MOR-7483.1, or MOR 7483.2.

6 In a further particular aspect, the present disclosure provides an isolated antibody that binds human 4-1 BB, comprising a heavy chain variable region that is the product of, or derived from, a human VH 3-23 gene, VH 1-69 gene, or VH 5. In another particular aspect the present disclosure provides an isolated antibody that binds human 4-1BB, 5 comprising a light chain variable region that is the product of, or derived from, a human VL A3 or Al-13 gene. In some embodiments, the isolated antibodies described herein have one or more of the following properties or characteristics: a) specifically bind to human 4-1BB; 10 b) bind to human and cynomolgus 4-1 BB; c) bind to human 4-1 BB or cynomolgus 4-1 BB but not rat, or mouse 4-1 BB; d) are an IgG, such as IgG1, IgG2, igG3, or IgG4; and e) are human antibodies, or humanized antibodies. In some other aspects, the present disclosure provides an antigen-binding portion 15 of any of the antibody provided by the present disclosure. In some embodiments, the antigen-binding portion is Fab or scFv fragment. In some further aspects, the present disclosure provides a derivative of any of the antibodies provided by the present disclosure. In some other aspects, the disclosure provides an isolated nucleic acid that 20 encodes a VH chain of an antibody or antigen-binding portion thereof that bind human 4 1BB1, which is selected from the group consisting of: (i) a nucleic acid sequence that encodes a VH chain amino acid sequence as set forth in SEQ ID NO:4, SEQ 1D NO:18, SEQ ID NO:32, or SEQ ID NO:43; (ii) a nucleic acid sequence as set forth in SEQ ID NO:11, SEQ ID NO:25, SEQ 25 ID NO:39, or SEQ ID NO:47; or (iii) a nucleic acid sequence that hybridizes under high stringency conditions to the complementary strand of a nucleic acid sequence as set forth in SEQ ID NO:11, SEQ ID NO:25, SEQ ID NO:39, or SEQ ID NO:47. In some other aspects, the disclosure provides an isolated nucleic acid that 30 encodes a VL chain of an antibody or antigen-binding portion thereof that binds human 4-1BB, which is selected from the group consisting of: (i) a nucleic acid sequence that encodes a VL chain amino acid sequence as set forth in SEQ ID NO:9, SEQ ID NO:23, SEQ ID NO:37, SEQ ID NO:45, SEQ ID NO:51, SEQ ID NO:56, SEQ ID NO:60, or SEQ ID NO:64; 7 (ii) a nucleic acid sequence as set forth in SEQ ID NO:12, SEQ ID NO:26, SEQ ID NO:40, SEQ ID NO:48, SEQ ID NO:53, SEQ ID NO:58, SEQ ID NO: 62, or SEQ ID NO:66; or (ii) a nucleic acid sequences that hybridizes under high stringency conditions to 5 the complementary strand of a nucleic acid sequence as set forth in SEQ ID NO:12, SEQ ID NO:26, SEQ ID NO:40, SEQ ID NO:48, SEQ ID NO:53, SEQ ID NO:58, SEQ ID NO: 62, or SEQ ID NO:66. In some further aspects, the disclosure provides a vector comprising any of the nucleic acids described herein. In a still further aspect, the disclosure provides a host 10 cell comprising any of the vectors described herein. Such host cells can be bacterial or mammalian. In some further aspects, the disclosure provides a pharmaceutical composition comprising any of the antibodies, antigen-binding portions thereof, or a derivative thereof, and a pharmaceutically acceptable carrier. 15 The disclosure further provides methods for treating abnormal cell growth in a subject in need thereof, comprising administering to the subject an effective amount of a binding molecule of the disclosure or pharmaceutical composition described herein. The disclosure further provides methods of reducing tumor cell metastasis in a subject, comprising administering to said subject an effective amount of a binding molecule, or 20 pharmaceutical compositions described herein. In a further aspect, the disclosure provides a use of any of the binding molecules, or a pharmaceutical composition described herein, for the manufacture of a medicament for the treatment of abnormal cell growth in a subject in need thereof. In a further aspect, the disclosure provides a binding molecule, or a pharmaceutical composition, as 25 described herein, for use in the treatment of abnormal cell growth in a subject in need thereof. In a yet further aspect, the disclosure provides a binding molecule, or a pharmaceutical composition, as described herein, for use in the treatment of tumor cell metastasis in a subject in need thereof. In a still further aspect, the disclosure provides a use of any of the binding molecules, or a pharmaceutical composition described 30 herein, for the manufacture of a medicament for the treatment of tumor cell metastasis in a subject in need thereof.

8 BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is four column graphs showing the mean fluorescence intensity of unstimulated (black) and PHA stimulated (light grey) primary PBMC from human (upper 5 left), cynomolgus (upper right), dog (lower left), and rat (lower right) incubated with the indicated 4-1BB antibody or control antibody conjugated to Alexafluor 647. The panel demonstrates binding to human and cynomolgus PBMC stimulated with PHA. Figure 2 is two line graphs showing luciferase reporter activity in 4-1BB expressing 293T cells that have been stimulated with several concentrations of 4-1BB 10 specific mAb or isotype control mAb. The left panel demonstrates reporter activity in cells that express cynomolgus 4-1BB. The right panel demonstrates activity in cells that express human 4-1 BB. The data is expressed as fold stimulation above isotype control. Figure 3 (3A and 3B) are line graphs showing the concentration of human IL-2 present in cell culture media following 72 hours of stimulation of human T cells with anti 15 CD3 and several concentrations of 4-1BB antibodies. Each panel (A and B) represents an individual donor. Figure 4 is a scattergram showing the expansion of human peripheral blood mononuclear cells in mice that have been treated with 4-1BB mAb or isotype control mAb. Data is expressed as the percentage of cells expressing human CD45 in the 20 peripheral blood of individual NSG mice on study days 24-28 that had been injected with six million human peripheral blood mononuclear cells on day 0 and injected with 1 mg/kg 4-1BB mAb or isotype control mAb on day 9. Statistical significance was determined using a two tailed Mann-Whitney test * p<0.05, ** p<0.005. No HBPT refers to animals that were not injected with human cells. 25 Figure 5 is two column graphs showing the change in proliferating CD8 central memory T cells at several time points following administration of 4-1BB mAb in cynomolgus monkeys. Data is shown as columns representing individual animals designated as (dose level-animal number) and is represented as intra-animal change in the number of Ki-67+ cells relative to pre study number {[(#Ki-67+ cells on indicated 30 study day - #Ki-67+ cells at pre dose)/#Ki-67+ cells at pre dose]l*100}. CD8 central memory cells were identified as CD3+, CD8+, CD28+ and CD95+. Figure 6 are line graphs showing the growth of tumors injected subcutaneously with tumor cells (PC3, left panel; LOVO, right panel) and human peripheral blood 9 mononuclear cells on study day 0. Mice were injected with 10 mg/kg of the indicated 4 1 BB mAbs on day 0. Figure 7 Left panel is a scattergram showing the percentage of PBMC that are positive for both the T cell surface marker CD8+ and have incorporated the BrdU 5 nucleoside analog following treatment of 4-1BB knock in mice with 4-1BB mAb or vehicle control. The right panel is a line graph showing the growth of murine melanoma tumors injected subcutaneously into 4-1BB knock in mice and treated with the indicated concentration of 4-1BB mAb. Figure 8 shows alignments of Amino Acid Sequences of the heavy Chain 10 Variable Regions and Light Chain Variable Regions (with CDRs underlined) with Relevant Germiline Sequences. DETAILED DESCRIPTION 15 A. DEFINITIONS Unless otherwise defined herein, scientific and technical terms used in connection with the present disclosure shall have the meanings that are commonly understood by those of ordinary skill in the art. Further, unless otherwise required by context, singular terms shall include pluralities and plural terms shall include the 20 singular. Generally, nomenclatures used in connection with, and techniques of, cell and tissue culture, molecular biology, immunology, microbiology, genetics and protein and nucleic acid chemistry and hybridization described herein are those well known and commonly used in the art. As used herein, each of the following terms has the meaning associated with it in 25 this section. The term "4-1 BB antibody" refers to an antibody, as defined herein, capable of binding to human 4-1BB receptor. The terms "4-1BB" and "4-1BB receptor" are used interchangeably in the present application, and include the human 4-1BB receptor, as well as variants, isoforms, and 30 species homologs thereof. Accordingly, a binding molecule, as defined and disclosed herein, may also bind 4-1BB from species other than human. In other cases, a binding molecule may be completely specific for the human 4-1 BB and may not exhibit species or other types of cross-reactivity.

10 The articles "a" and "an" refer to one or to more than one (i.e., to at least one) of the grammatical object of the article. By way of example, "an element" means one element or more than one element. The term "agonist" refers to a binding molecule, as defined herein, which upon 5 binding to 4-1BB, (1) stimulates or activates 4-1BB, (2) enhances, increases, promotes, induces, or prolongs an activity, function, or presence of 4-1BB, or (3) enhances, increases, promotes, or induces the expression of 4-1BB. The term "amino acid" refers to naturally occurring and synthetic amino acids, as well as amino acid analogs and amino acid mimetics that function similarly to the 10 naturally occurring amino acids. Naturally occurring amino acids are those encoded by the genetic code, as well as those amino acids that are later modified, e.g., hydroxyproline, gamma-carboxyglutamate, and 0-phosphoserine. The term "amino acid analogs" refers to compounds that have the same basic chemical structure as a naturally occurring amino acid but the C-terminal carboxy group, the N-terminal amino 15 group, or side chain functional group has been chemically modified to another functional group. The term "amino acid mimetics" refers to chemical compounds that have a structure that is different from the general chemical structure of an amino acid, but that functions similarly to a naturally occurring amino acid. The term "antibody" is an art-recognized term and refers to an antigen-binding 20 protein (i.e, immunoglobulin) having a basic four-polypeptide chain structure consisting of two identical heavy (H) chains and two identical light (L) chains. Each L chain is linked to an H chain by one covalent disulfide bond, while the two H chains are linked to each other by one or more disulfide bonds depending on the H chain isotype. Each heavy chain has, at the N-terminus, a variable region (abbreviated herein as VH) 25 followed by a constant region. The heavy chain constant region is comprised of three domains, CHI, CH2 and CH3. Each light chain has, at the N-terminus, a variable region (abbreviated herein as VL) followed by a constant region at its other end. The light chain constant region is comprised of one domain, CL. The VL is aligned with the VH and the CL is aligned with the first constant domain of the heavy chain (CHI). The pairing of a VH 30 and VL together forms a single antigen-binding site. An IgM antibody consists of 5 of the basic heterotetramer units along with an additional polypeptide called J chain, and therefore contains 10 antigen binding sites, while secreted IgA antibodies can polymerize to form polyvalent assemblages comprising 2-5 of the basic 4-chain units along with J chain.

11 The VH and VL regions can be further subdivided into regions of hypervariability, termed complementarity determining regions (CDR), interspersed with regions that are more conserved, termed framework regions (FR). The CDR regions can be determined using the Kabat or Chothia numbering systems, both of which are well known to those of 5 skill in the art. See, e.g. Kabat, E. A., et al. (1991) Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242; Chothia and Lesk, J. Mol. Biol. 196:901-917 (1987). Each VH and VL is composed of three CDRs and four FRs, arranged from amino terminus to carboxy-terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, 10 CDR3, FR4. Throughout the present disclosure, the three CDRs of the heavy chain are referred to as H-CDR1, H-CDR2, and H-CDR3. Similarly, the three CDRs of the light chain are referred to as L-CDR1, L-CDR2, and L-CDR3. The variable regions of the heavy and light chains contain a binding domain that interacts with an antigen. The constant regions of the antibodies may mediate the binding of the immunoglobulin to 15 host tissues or factors, including various cells of the immune system (e.g., effector cells) and the first component (Clq) of the classical complement system. Within light and heavy chains, the variable and constant regions are joined by a "J region of about 12 or more amino acids, with the heavy chain also including a "D" region of about 10 or more amino acids. See generally, Fundamental Immunology Ch. 7 (Paul, W., ed., 2 nd ed. 20 Raven Press, N.Y. (1989)). The L chain from any vertebrate species can be assigned to one of two clearly distinct types, called kappa and lambda, based on the amino acid sequences of their constant domains. Depending on the amino acid sequence of the constant domain of their heavy chains (CH), antibodies can be assigned to different classes or isotypes. 25 There are five classes of antibodies: IgA, lgD, IgE, IgG, and IgM, having heavy chains designated a (alpha), 6 (delta), E (epsilon), y (gamma), and p (mu), respectively. The IgG class of antibody can be further classified into four subclasses IgG1, igG2, IgG3, and IgG4 by the gamma heavy chains, Y1 - Y4, respectively. The term "antibody derivative" or "derivative" of an antibody refers to a molecule 30 that is capable of binding to the same antigen (e.g., 4-1BB) that the antibody binds to and comprises an amino acid sequence of the antibody linked to an additional molecular entity. The amino acid sequence of the antibody that is contained in the antibody derivative may be a full-length heavy chain, a full-length light chain, any portion or portions of a full-length heavy chain, any portion or portions of the full-length light chain 12 of the antibody, any other fragment(s) of an antibody, or the complete antibody. The additional molecular entity may be a chemical or biological molecule. Examples of additional molecular entities include chemical groups, amino acids, peptides, proteins (such as enzymes, antibodies), and chemical compounds. The additional molecular 5 entity may have any utility, such as for use as a detection agent, label, marker, pharmaceutical or therapeutic agent. The amino acid sequence of an antibody may be attached or linked to the additional molecular entity by chemical coupling, genetic fusion, noncovalent association, or otherwise. The term "antibody derivative" also encompasses chimeric antibodies, humanized antibodies, and molecules that are 10 derived from modifications of the amino acid sequences of a 4-1BB antibody, such as conservation amino acid substitutions, additions, and insertions. The term "antigen-binding fragment" or "antigen binding portion" of an antibody refers to one or more portions of an antibody that retain the ability to bind to the antigen that the antibody bonds to (e.g., 4-1BB). Examples of "antigen-binding fragment" of an 15 antibody include (i) a Fab fragment, a monovalent fragment consisting of the VL, VH, CL and CHI domains; (ii) a F(ab') 2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; (iii) a Fd fragment consisting of the VH and CH1 domains; (iv) a Fv fragment consisting of the VL and VH domains of a single arm of an antibody, (v) a dAb fragment (Ward et al., Nature 341:544-546 (1989)), 20 which consists of a VH domain; and (vi) an isolated complementarity determining region (CDR). The term "binding molecule" encompasses (1) antibody, (2) antigen-binding fragment of an antibody, and (3) derivative of an antibody, each as defined herein. The term "binding 4-1BB," "binds 4-1BB," "binding to 4-1BB," or "binds to 4-1BB" 25 refers to the binding of a binding molecule, as defined herein, to the human 4-1BB in an in vitro assay, such as a BlAcore assay as described in Example 6, with an affinity (KD) of 500 nM or less. The term "chimeric antibody" refers to an antibody that comprises amino acid sequences derived from different animal species, such as those having a variable region 30 derived from a human antibody and a murine immunoglobulin constant region. The term "compete for binding" refers to the interaction of two antibodies in their binding to a binding target. A first antibody competes for binding with a second antibody if binding of the first antibody with its cognate epitope is detectably decreased in the presence of the second antibody compared to the binding of the first antibody in the 13 absence of the second antibody. The alternative, where the binding of the second antibody to its epitope is also detectably decreased in the presence of the first antibody, can, but need not, be the case. That is, a first antibody can inhibit the binding of a second antibody to its epitope without that second antibody inhibiting the binding of the 5 first antibody to its respective epitope. However, where each antibody detectably inhibits the binding of the other antibody with its cognate epitope, whether to the same, greater, or lesser extent, the antibodies are said to "cross-compete" with each other for binding of their respective epitope(s). The term "epitope" refers to a part of an antigen to which an antibody (or antigen 10 binding fragment thereof) binds. Epitopes can be formed both from contiguous amino acids or noncontiguous amino acids juxtaposed by tertiary folding of a protein. Epitopes formed from contiguous amino acids are typically retained on exposure to denaturing solvents whereas epitopes formed by tertiary folding are typically lost on treatment with denaturing solvents. An epitope can include various numbers of amino acids in a unique 15 spatial conformation. Methods of determining spatial conformation of epitopes include, for example, x-ray crystallography and 2-dimensional nuclear magnetic resonance. See, e.g., Epitope Mapping Protocols in Methods in Molecular Biology, Vol. 66, G. E. Morris, Ed. (1996). Once a desired epitope on an antigen is determined, antibodies to that epitope can be generated, e.g., using the techniques described herein. The generation 20 and characterization of antibodies may also elucidate information about desirable epitopes. From this information, it is then possible to competitively screen antibodies for binding to the same epitope. An approach to achieve this is to conduct cross competition studies to find antibodies that competitively bind with one another, i.e., the antibodies compete for binding to the antigen. A high throughput process for "binning" 25 antibodies based upon their cross-competition is described in PCT Publication No. WO 03/48731. The term "germline" refers to the nucleotide sequences of the antibody genes and gene segments as they are passed from parents to offspring via the germ cells. The germline sequence is distinguished from the nucleotide sequences encoding 30 antibodies in mature B cells which have been altered by recombination and hypermutation events during the course of B cell maturation. The term "glycosylation sites" refers to amino acid residues which are recognized by a eukaryotic cell as locations for the attachment of sugar residues. The amino acids where carbohydrate, such as oligosaccharide, is attached are typically asparagine (N- 14 linkage), serene (0-linkage), and threonine (0-linkage) residues. The specific site of attachment is typically signaled by a sequence of amino acids, referred to herein as a "glycosylation site sequence". The glycosylation site sequence for N-linked glycosylation is: -Asn-X-Ser- or -Asr-X-Thr-, where X may be any of the conventional amino acids, 5 other than proline. The terms "N-linked" and "O-linked" refer to the chemical group that serves as the attachment site between the sugar molecule and the amino acid residue. N-linked sugars are attached through an amino group; O-linked sugars are attached through a hydroxyl group. The term "glycan occupancy" refers to the existence of a carbohydrate moiety linked to a glycosylation site (i.e., the glycan site is occupied). 10 Where there are at least two potential glycosylation sites on a polypeptide, either none (0-glycan site occupancy), one (1-glycan site occupancy) or both (2-glycan site occupancy) sites can be occupied by a carbohydrate moiety. The term "host cell" refers to a cellular system which can be engineered to generate proteins, protein fragments, or peptides of interest. Host cells include, without 15 limitation, cultured cells, e.g., mammalian cultured cells derived from rodents (rats, mice, guinea pigs, or hamsters) such as CHO, BHK, NSO, SP2/0, YB2/0; or human tissues or hybridoma cells, yeast cells, and insect cells, and cells comprised within a transgenic animal or cultured tissue. The term encompasses not only the particular subject cell but also the progeny of such a cell. Because certain modifications may occur in 20 succeeding generations due to either mutation or environmental influences, such progeny may not be identical to the parent cell, but are still included within the scope of the term "host cell." The term "human antibody" refers to an antibody in which the entire amino acid sequences of the light chains and heavy chains are from the human immunoglobulin 25 genes. A human antibody may contain murine carbohydrate chains if produced in a mouse, in a mouse cell or in a hybridoma derived from a mouse cell. Human antibodies may be prepared in a variety of ways known in the art. The term "humanized antibody" refers to a chimeric antibody that contains amino acid residues derived from human antibody sequences. A humanized antibody may 30 contain some or all of the CDRs from a non-human animal antibody while the framework and constant regions of the antibody contain amino acid residues derived from human antibody sequences. The term "illustrative antibody" refers to any one of the antibodies described in the disclosure and designated as MOR-6032, MOR-7361, MOR-7480, MOR-7480.1, 15 MOR-7480.2, MOR-7483, MOR-7483.1, and MOR-7483.2. These antibodies may be in any class (e.g., IgA, IgD, IgE, IgG, and igM). Thus, each antibody identified above encompasses antibodies in all five classes that have the same amino acid sequences for the VL and V regions. Further, the antibodies in the IgG class may be in any 5 subclass (e.g., IgG1 igG2, igG3, and igG4). Thus, each antibody identified above in the IgG subclass encompasses antibodies in all four subclasses that have the same amino acid sequences for the Vt and VH regions. The amino acid sequences of the heavy chain constant regions of human antibodies in the five classes, as well as in the four IgG subclasses, are known in the art. As examples, the amino acid sequences of the 10 human IgG1 and igG2 constant regions are provided in SEQ ID NOs: 69 and 71, respectively. The amino acid sequence of the full length heavy chain for the IgG2 subclass of each of the illustrative antibodies is provided in the disclosure. The term "isolated antibody" or "isolated binding molecule" refers to an antibody or a binding molecule, as defined herein, that: (1) is not associated with naturally 15 associated components that accompany it in its native state; (2) is free of other proteins from the same species; (3) is expressed by a cell from a different species; or (4) does not occur in nature. Examples of isolated antibodies include a 4-1 BB antibody that has been affinity purified using 4-1BB, a 4-1BB antibody that has been generated by hybridomas or other cell line in vitro, and a 4-1BB antibody derived from a transgenic 20 animal. The term "isolated nucleic acid" refers to a nucleic acid molecule of genomic, cDNA, or synthetic origin, or a combination thereof, which is separated from other nucleic acid molecules present in the natural source of the nucleic acid. For example, with regard to genomic DNA, the term "isolated" includes nucleic acid molecules which 25 are separated from the chromosome with which the genomic DNA is naturally associated. Preferably, an "isolated" nucleic acid is free of sequences which naturally flank the nucleic acid (i.e., sequences located at the 5' and 3 ends of the nucleic acid of interest. The term *ka" refers to the association rate constant of a particular antibody 30 antigen interaction, whereas the term "kd" refers to the dissociation rate constant of a particular antibody-antigen interaction. The term "Ko" refers to the equilibrium dissociation constant of a particular antibody-antigen interaction. It is obtained from the ratio of kd to k. (i.e,. kdka) and is expressed as a molar concentration (M). Ko is used as a measure for the affinity of an 16 antibody's binding to its binding partner. The smaller the KD, the more tightly bound the antibody is, or the higher the affinity between antibody and the antigen. For example, an antibody with a nanomolar (nM) dissociation constant binds more tightly to a particular antigen than an antibody with a micromoiar (pM) dissociation constant. KD values for 5 antibodies can be determined using methods well established in the art. One method for determining the KD of an antibody is by using surface plasmon resonance, typically using a biosensor system such as a Biacore* system. An assay procedure using the BIACOREW system (BlAcore assay) is described in the Examples section of this disclosure. 10 The term "mammal" refers to any animal species of the Mammalia class. Examples of mammals include: humans; laboratory animals such as rats, mice, simians and guinea pigs; domestic animals such as cats, dogs, rabbits, cattle, sheep, goats, horses, and pigs; and captive wild animals such as lions, tigers, elephants, and the like. The term "monoclonal antibody" refers to an antibody obtained from a population 15 of substantially homogeneous antibodies, i.e., the individual antibodies comprising the population are identical except for possible naturally occurring mutations that may be present in minor amounts. Monoclonal antibodies are highly specific, being directed against a single antigenic site. Furthermore, in contrast to polyclonal antibody preparations which include different antibodies directed against different determinants 20 (epitopes), each monoclonal antibody is directed against a single determinant on the antigen. In addition to their specificity, the monoclonal antibodies are advantageous in that they may be synthesized uncontaminated by other antibodies. The modifier "monoclonal" is not to be construed as requiring production of the antibody by any particular method. For example, the monoclonal antibodies may be prepared by the 25 hybridoma methodology or may be made using recombinant DNA methods in bacterial, eukaryotic animal or plant cells (see, e.g., U.S. Pat. No. 4,816,567). Monoclonal antibodies may also be isolated from phage antibody libraries using the techniques described in Clackson et al., Nature, 352:624-628 (1991) and Marks et al., J. Mol. Biol., 222:581-597 (1991), for example. 30 The term "prevent" or "preventing," with reference to a certain disease condition in a mammal, refers to preventing or delaying the onset of the disease, or preventing the manifestation of clinical or subclinical symptoms thereof. The term "recombinant antibody" refers to an antibody that is prepared, expressed, created or isolated by recombinant means, such as antibodies isolated from 17 an animal that is transgenic for another species' immunoglobulin genes, antibodies expressed using a recombinant expression vector transfected into a host cell, antibodies isolated from a recombinant, combinatorial antibody library, or antibodies prepared, expressed, created or isolated by any other means that involves splicing of 5 immunoglobulin gene sequences to other DNA sequences. As used herein, "sequence identity" between two polypeptide sequences indicates the percentage of amino acids that are identical between the sequences. The amino acid sequence identity of polypeptides can be determined conventionally using known computer programs such as Bestfit, FASTA, or BLAST (see, e.g. Pearson, Methods 10 Enzymol. 183:63-98 (1990); Pearson, Methods Mo. Biel. 132:185-219 (2000); Altschul et al., J. Moi. Bio. 215:403-410 (1990); Altschul et al., Nucelic Acids Res. 25:3389-3402 (1997)). When using Bestfit or any other sequence alignment program to determine whether a particular sequence is, for instance, 95% identical to a reference amino acid sequence, the parameters are set such that the percentage of identity is calculated over 15 the full length of the reference amino acid sequence and that gaps in homology of up to 5% of the total number of amino acid residues in the reference sequence are allowed. This aforementioned method in determining the percentage of identity between polypeptides is applicable to all proteins, fragments, or variants thereof disclosed herein. The term "specifically binds" or "specifically binds to," in reference to the 20 interaction of a binding molecule, as defined herein, (e.g., an antibody) with its binding partner (e.g., an antigen), refers to the ability of the binding molecule to discriminate between an antigen of interest from an animal species and the antigen orthologue from a different animal species under a given set of conditions. A 4-1BB binding molecule is said to specifically bind to human 4-1 BB if it binds to human 4-1 BB at an EC50 that is 25 below 50 percent of the EC50 at which it binds 4-1BB of rat or mouse as determined in an in vitro assay . Binding specificity of an antibody can be determined using methods known in the art. Examples of such methods include FACS using PHA stimulated primary cells, Western blots, ELISA, RIA-, ECL-, IRMA-tests and peptide scans. The term "selectively binds* or "selectively binds to," in reference to the 30 interaction of a binding molecule, as defined herein, (e.g., an antibody) with its binding partner (e.g., an antigen), refers to the ability of the binding molecule to discriminate between an antigen of interest from an animal species (such as human 4-1 BB) and a different antigen from the same animal species (such as human CD40) under a given set of conditions. A 4-1 BB binding molecule is said to selectively bind to human 4-1 BB 18 if it binds to human 4-IBB at an EC50 that is below 10 percent of the EC50 at which it binds to human CD40 or human CD134 as determined in an in vitro assay. The term "treat", "treating", or "treatment", with reference to a certain disease condition in a mammal, refers causing a desirable or beneficial effect in the mammal 5 having the disease condition. The desirable or beneficial effect may include reduced frequency or severity of one or more symptoms of the disease (i.e., tumor growth and/or metastasis, or other effect mediated by the numbers and/or activity of immune cells, and the like), or arrest or inhibition of further development of the disease, condition, or disorder. In the context of treating cancer in a mammal, the desirable or beneficial 10 effect may include inhibition of further growth or spread of cancer cells, death of cancer cells, inhibition of reoccurrence of cancer, reduction of pain associated with the cancer, or improved survival of the mammal. The effect can be either subjective or objective. For example, if the mammal is human, the human may note improved vigor or vitality or decreased pain as subjective symptoms of improvement or response to therapy. 15 Alternatively, the clinician may notice a decrease in tumor size or tumor burden based on physical exam, laboratory parameters, tumor markers or radiographic findings. Some laboratory signs that the clinician may observe for response to treatment include normalization of tests, such as white blood cell count, red blood cell count, platelet count, erythrocyte sedimentation rate, and various enzyme levels. Additionally, the 20 clinician may observe a decrease in a detectable tumor marker. Alternatively, other tests can be used to evaluate objective improvement, such as sonograms, nuclear magnetic resonance testing and positron emissions testing. The term "vector" refers to a nucleic acid molecule capable of transporting a foreign nucleic acid molecule. The foreign nucleic acid molecule is linked to the vector 25 nucleic acid molecule by a recombinant technique, such as ligation or recombination. This allows the foreign nucleic acid molecule to be multiplied, selected, further manipulated or expressed in a host cell or organism. A vector can be a plasmid, phage, transposon, cosmid, chromosome, virus, or virion. One type of vectors can be integrated into the genome of a host cell upon introduction into the host cell, and thereby are 30 replicated along with the host genome (e.g., non-episomal mammalian vectors). Another type of vectors are capable of autonomous replication in a host cell into which it is introduced (e.g., bacterial vectors having a bacterial origin of replication and episomal mammalian vectors). Another specific type of vectors capable of directing the expression of expressible foreign nucleic acids to which they are operatively linked are 19 commonly referred to "expression vectors." Expression vectors generally have control sequences that drive expression of the expressible foreign nucleic acids. Simpler vectors, known as "transcription vectors," are only capable of being transcribed but not translated: they can be replicated in a target cell but not expressed. The term "vector" 5 encompasses all types of vectors regardless of their function. Vectors capable of directing the expression of expressible nucleic acids to which they are operatively linked are commonly referred to "expression vectors." The methods and techniques of the present disclosure are generally performed according to methods well known in the art and as described in various general and 10 more specific references that are cited and discussed throughout the present specification unless otherwise indicated. Such references include, e.g., Sambrook and Russell, Molecular Cloning, A Laboratory Approach, Cold Spring Harbor Press, Cold Spring Harbor, NY (2001), Ausubel et al., Current Protocols in Molecular Biology, John Wiley & Sons, NY (2002), and Harlow and Lane Antibodies: A Laboratory Manual, Cold 15 Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1990). Enzymatic reactions and purification techniques are performed according to manufacturer's specifications, as commonly accomplished in the art or as described herein. The nomenclatures used in connection with, and the laboratory procedures and techniques of, analytical chemistry, synthetic organic chemistry, and medicinal and pharmaceutical chemistry described 20 herein are those well known and commonly used in the art. Standard techniques are used for chemical syntheses, chemical analyses, pharmaceutical preparation, formulation, and delivery, and treatment of patients. As used herein, the twenty conventional amino acids and their abbreviations follow conventional usage. See Immunology-A Synthesis (2nd Edition, E. S. Golub and D. R. 25 Gren, Eds., Sinauer Associates, Sunderland, Mass. (1991)). B. BINDING MOLECULES THAT BIND TO HUMAN 4-1 BB The present disclosure provides isolated binding molecules that bind to human 4 1 BB, including 4-1BB antibodies, antigen-binding fragments of the 4-1 BB antibodies, 30 and derivatives of the 4-1BB antibodies. B-1. 4-1 BB ANTIBODIES In some aspects, the present disclosure provides an isolated antibody that binds to human 4-1BB at an epitope within amino acid residues 115 - 156 of SEQ ID No: 68. In some embodiments, the isolated antibody comprises the H-CDR1 amino acid 20 sequence of SEQ ID NO: 29, H-CDR2 amino acid sequence of SEQ ID NO: 30, and H CDR3 amino acid sequence of SEQ ID NO: 31. In some other embodiments, the isolated antibody comprises the L-CDR1 amino acid sequence of SEQ ID NO: 34, L CDR2 amino acid sequence of SEQ ID NO: 35, and L-CDR3 amino acid sequence of 5 SEQ ID NO: 36. In some other embodiments, the antibodies described herein above have one or more biological properties described herein below. In other aspects, the disclosure provides an isolated antibody that binds to human 4-1BB, wherein said antibody comprises: (a) an H-CDR1 as set forth in SEQ ID NO:1 , SEQ ID NO:15, or SEQ ID NO:29; (b) an H-CDR2 as set forth in SEQ ID NO:2, 10 SEQ ID NO:16, or SEQ ID NO:30; and (c) an H-CDR3 as set forth in SEQ ID NO:3, SEQ ID NO: 17, or SEQ ID NO:31. In another aspect, the disclosure provides an isolated antibody that binds to human 4-1BB, wherein said antibody comprises: (a) an L-CDR1 as set forth in SEQ ID NO:6, SEQ ID NO: 20, or SEQ ID NO:34; (b) an L-CDR2 as set forth in SEQ ID NO:7, 15 SEQ ID NO:21, or SEQ ID NO:35; and (c) an L-CDR3 as set forth in SEQ ID NO:8, SEQ ID NO:22, SEQ ID NO:36, or SEQ ID NO: 55. In a further aspect, the disclosure provides an isolated antibody that binds to human 4-1BB, wherein said antibody comprises: (a) an H-CDR1 as set forth in SEQ ID NO:1, SEQ ID NO:15, or SEQ ID NO:29; (b) an H-CDR2 as set forth in SEQ ID NO:2, 20 SEQ ID NO:16, or SEQ ID NO:30; and (c) an H-CDR3 as set forth in SEQ ID NO:3, SEQ ID NO: 17, or SEQ ID NO:31; and further comprises: (d) an L-CDR1 as set forth in SEQ ID NO:6, SEQ ID NO: 20, or SEQ ID NO:34; (e) an L-CDR2 as set forth in SEQ ID NO:7, SEQ ID NO:21, or SEQ ID NO:35; and (f) an L-CDR3 as set forth in SEQ ID NO:8, SEQ ID NO:22, SEQ ID NO:36, or SEQ ID NO: 55. 25 In some further aspects, the disclosure provides an isolated antibody that binds to human 4-1BB, wherein said antibody is selected from the group consisting of: (a) an antibody comprising an H-CDR1 as set forth in SEQ ID NO:1, an H-CDR2 as set forth in SEQ ID NO:2, and an H-CDR3 as set forth in SEQ ID NO:3; (b) an antibody comprising an H-CDR1 as set forth in SEQ ID NO:15, an H-CDR2 30 as set forth in SEQ ID NO:16, and an H-CDR3 as set forth in SEQ ID NO:17; and (c) an antibody comprising an H-CDR1 as set forth in SEQ ID NO:29, an H-CDR2 as set forth in SEQ ID NO:30, and an H-CDR3 as set forth in SEQ ID NO:31. In some further aspects, the disclosure provides an isolated antibody that binds to human 4-16B, wherein said antibody is selected from the group consisting of: 21 (a) an antibody comprising an L-CDRI as set forth in SEQ ID NO:6, an L-CDR2 as set forth in SEQ ID NO:7, and an L-CDR3 as set forth in SEQ ID NO:8; (b) an antibody comprising an L-CDR1 as set forth in SEQ ID NO:20, an L-CDR2 as set forth in SEQ ID NO:21, and an L-CDR3 as set forth in SEQ ID NO:22; 5 (c) an antibody comprising an L-CDR1 as set forth in SEQ ID NO:34, an L-CDR2 as set forth in SEQ ID NO:35, and an L-CDR3 as set forth in SEQ ID NO:36; and (d) an antibody comprising an L-CDR1 as set forth in SEQ ID NO:34, an L-CDR2 as set forth in SEQ ID NO:35, and an L-CDR3 as set forth in SEQ ID NO:55. In some further aspects, the disclosure provides an isolated antibody that binds 10 to the human 4-1BB, wherein said antibody is selected from the group consisting of: (a) an antibody comprising an H-CDR1 as set forth in SEQ ID NO:1, an H-CDR2 as set forth in SEQ ID NO:2, an H-CDR3 as set forth in SEQ ID NO:3; an L-CDR1 as set forth in SEQ ID NO:6, an L-CDR2 as set forth in SEQ ID NO:7, and an L-CDR3 as set forth in SEQ ID NO:8; 15 (b) an antibody comprising an H-CDR1 as set forth in SEQ ID NO:15, an H-CDR2 as set forth in SEQ ID NO:16, an H-CDR3 as set forth in SEQ ID NO:17; an L-CDR1 as set forth in SEQ ID NO:20, an L-CDR2 as set forth in SEQ ID NO:21, and an L-CDR3 as set forth in SEQ ID NO:22; (c ) an antibody comprising an H-CDR1 as set forth in SEQ ID NO:29, an H 20 CDR2 as set forth in SEQ ID NO:30, an H-CDR3 as set forth in SEQ ID NO:31; an L CDRI as set forth in SEQ ID NO:34, an L-CDR2 as set forth in SEQ ID NO:35, and an L-CDR3 as set forth in SEQ ID NO:36; and (d) an antibody comprising an H-CDR1 as set forth in SEQ ID NO:29, an H-CDR2 as set forth in SEQ ID NO:30, an H-CDR3 as set forth in SEQ ID NO:31; an L-CDR1 as 25 set forth in SEQ ID NO:34, an L-CDR2 as set forth in SEQ ID NO:35, and an L-CDR3 as set forth in SEQ ID NO:55. In a further aspect, the disclosure provides an isolated antibody that binds to human 4-1BB, wherein said antibody comprises a VH chain amino acid sequence as set forth in SEQ ID NO:4, SEQ ID NO:18, SEQ ID NO:32, or SEQ ID NO:43 30 In a further aspect, the disclosure provides an isolated antibody that binds to human 4-11BB, wherein said antibody comprises a VL chain amino acid sequence as set forth in SEQ ID NO:9, SEQ ID NO:23, SEQ ID NO:37, SEQ ID NO:45, SEQ ID NO:51, SEQ ID NO:56, SEQ ID NO:60, or SEQ ID NO:64.

22 In a further aspect, the disclosure provides an isolated antibody that binds to human 4-1BB, wherein said antibody comprises (1) a VH chain amino acid sequence as set forth in SEQ ID NO:4, SEQ ID NO:18, SEQ ID NO:32, or SEQ ID NO:43, and (2) a VL chain amino acid sequence as set forth in SEQ ID NO:9, SEQ ID NO:23, SEQ ID 5 NO:37, SEQ ID NO:45, SEQ ID NO:51, SEQ ID NO:56, SEQ ID NO:60, or SEQ ID NO:64. In a further aspect, the disclosure provides an isolated antibody that binds to human 4-1 BB, wherein said antibody is selected from the group consisting of: (a) an antibody comprising a VH chain amino acid sequence as set forth in SEQ 10 ID NO:4 and a VL chain amino acid sequence as set forth in SEQ ID NO:9; (b) an antibody comprising a VH chain amino acid sequence as set forth in SEQ ID NO:18 and a VL chain amino acid sequence as set forth in SEQ ID NO:23; (c) an antibody comprising a VH chain amino acid sequence as set forth in SEQ ID NO:32 and a VL chain amino acid sequence as set forth in SEQ ID NO:37 or SEQ ID 15 NO 56; and (d) an antibody comprising a VH chain amino acid sequence as set forth in SEQ ID NO:43 and a VL chain amino acid sequence as set forth in SEQ ID NO:45, SEQ ID NO:51, SEQ ID NO: 60, or SEQ ID NO: 64. In some embodiments, the antibodies described herein above, including 20 antibodies described with reference to epitope binding and antibodies described with reference to specific amino acid sequences of CDRs or variable regions, have at least one of the following functional properties: (a) bind to human 4-1 BB with a Ko of 500 nM or less; (b) have agonist activity on human 4-1BB; (c) do not bind to human CD40 receptor at concentration up to 1000 nM; (d) do not bind to human CD134 receptor at 25 concentrations up to 1000 nM; (e) do not bind to rat, or mouse 4-1BB at concentrations up to 100 nM; (h) are capable of inhibiting tumor cell growth; and (i) have therapeutic effect on a cancer. In some further embodiments, the antibodies specifically bind to human 4-1BB with a K 0 of 500 nM or less, 100 nM or less, 50 nM or less, 10 nM or less, 5 nM or less, or 1 nM or less, for the human 4-1BB extracellular domain as measured 30 with the BIACore assay described in this disclosure. In still further embodiments, the antibody is a human antibody or humanized antibody that specifically binds to human 4 1BB with a KD of 500 nM or less, 100 nM or less, 50 nM or less, 10 nM or less, 5 nM or less, or 1 nM or less, for the human 4-1BB extracellular domain as measured with the 23 BIACore assay described in this disclosure. In some further embodiments, the antibody is a human antibody that specifically and selectively binds to human 4-1 BB. In other embodiments, the antibodies described herein above comprise a heavy chain variable region from a particular germiline heavy chain immunoglobulin gene 5 and/or a light chain variable region from a particular germline light chain immunoglobulin gene, such as an antibody comprising a heavy chain variable region that is the product of, or derived from, a human VH 1-69 gene, VH 3-23 gene, or VH 5 gene. Exemplary antibodies include MOR-7480.1, MOR-7480.2, MOR-7483.1, and MOR-7483.2, each of which contains amino acids that derived from human germline VHS gene. 10 In still other embodiments, the antibodies described herein above comprise a light chain variable region that is derived from a human VL A3 gene. In yet other embodiment the antibodies described herein above comprise a heavy chain variable region that is the product of, or derived from, a human VH 1-69 gene, VH 3-23 gene, or VH 5 gene, and further comprise a light chain variable region that is the product of, or 15 derived from, a human VL A3 gene, wherein the antibody or portion thereof specifically binds to human 4-1BB. Exemplary antibodies include MOR-7480.1, MOR-7480.2, MOR-7483.1, and MOR-7483.2, each of which contains amino acids that derived from human germline VHS gene and VL A3 gene, respectively. As used herein, a human antibody comprises heavy or light chain variable 20 regions that is *derived from" a particular germline sequence if the variable regions of the antibody are obtained from a system that uses human germline immunoglobulin genes. Such systems include immunizing a transgenic mouse carrying human immunoglobulin genes with the antigen of interest or screening a human immunoglobulin gene library displayed on phage with the antigen of interest. A human 25 antibody that is "derived from" a human germline immunoglobulin sequence can be identified as such by comparing the amino acid sequence of the human antibody to the amino acid sequences of human germiine immunoglobulins and selecting the human germline immunoglobulin sequence that is closest in sequence (i.e., greatest % identity) to the sequence of the human antibody. A human antibody that is "derived from" a 30 particular human germline immunoglobulin sequence may contain amino acid differences as compared to the germline sequence, due to, for example, naturally occurring somatic mutations or intentional introduction of site-directed mutation. However, a selected human antibody typically is at least 90% identical in amino acid sequence to an amino acid sequence encoded by a human germline immunoglobulin 24 gene and contains amino acid residues that identify the human antibody as being human when compared to the germline immunoglobulin amino acid sequences of other species (e.g., murine germline sequences). In certain cases, a human antibody may be at least 95%, or even at least 96%, 97%, 98%, or 99% identical in amino acid sequence 5 to the amino acid sequence encoded by the germline immunoglobulin gene. In certain cases, the human antibody is identical in amino acid sequence to the amino acid sequence encoded by the germline Ig gene. Typically, a human antibody derived from a particular human germline sequence will display no more than 10 amino acid differences from the amino acid sequence encoded by the human germline immunoglobulin gene. 10 In certain cases, the human antibody may display no more than 5, or even no more than 4, 3, 2, or 1 amino acid differences from the amino acid sequence encoded by the germline immunoglobulin gene. Alignments of the amino acid sequences of variable regions of the illustrative antibodies and the relevant germlines are provided in Figure 6. 15 In another aspect, the disclosure provides isolated antibodies that compete or cross-compete for binding to human 4-1BB with any of the illustrative antibodies of the disclosure, such as MOR-6032, MOR-7361, MOR-7480, MOR-7480.1, MOR-7480.2, MOR-7483, MOR-7483.1, or MOR-7483.2. In a particular embodiment, the disclosure provides isolated antibodies that compete or cross-compete for binding to the same 20 epitope on the human 4-1BB with any of the illustrative antibodies of the disclosure. The ability of an antibody to compete cross-compete for binding with another antibody can be determined using standard binding assays known in the art, such as BlAcore analysis, ELISA assays, or flow cytometry. For example, one can allow an illustrative antibody of the disclosure to bind to human 4-1 BB under saturating conditions and then 25 measure the ability of the test antibody to bind to the 4-iBB. If the test antibody is able to bind to the 4-1 BB at the same time as the illustrative antibody, then the test antibody binds to a different epitope as the illustrative antibody. However, if the test antibody is not able to bind to the 4-1 BB at the same time, then the test antibody binds to the same epitope, an overlapping epitope, or an epitope that is in close proximity to the epitope 30 bound by the illustrative antibody. This experiment can be performed using various methods, such as ELISA, RIA, FACS or surface plasmon resonance. The 4-1BB antibodies described herein can be in any class, such as igG, IgM, IgE, IgA, or igD. It is preferred that the 4-1BB antibodies are in the igG class, such as IgG1, IgG2, igG3, or IgG4 subclass, more preferably IgG2 subclass. A 4-1BB antibody 25 can be converted from one class or subclass to another class or subclass using methods known in the art. An exemplary method for producing an antibody in a desired class or subclass comprises the steps of isolating a nucleic acid encoding a heavy chain of an 4-1BB antibody and a nucleic acid encoding a light chain of a 4-1BB antibody, 5 isolating the sequence encoding the VH region, ligating the VH sequence to a sequence encoding a heavy chain constant region of the desired class or subclass, expressing the light chain gene and the heavy chain construct in a cell, and collecting the 4-11BB antibody. Further, the antibodies provided by the present disclosure can be monoclonal or 10 polyclonal, but preferably monoclonal. Examples of specific isolated antibodies provided by the present disclosure include the following illustrative antibodies: MOR-6032, MOR-7361, MOR-7480, MOR 7480.1, MOR-7480.1, MOR-7480.2, MOR-7483, MOR-7483, MOR-7483.1, and MOR 7483.2. The nucleotide and amino acid sequences of the heavy chain variable region, 15 full length heavy chain for the igG2 subclass, light chain variable region, and full length light chain of these antibodies are provided in this disclosure; an index of the SEQ ID NOs for these sequences is provided in Table 1. The amino acid sequences of the CDRs of these illustrative antibodies are shown in Table 2. 20 26 Table: 1. Index of SEQ ID NOs Antibody Chain Full length Variable Region Amino Acid Nucleotide Amino Acid Nucleotide SEQ ID SEQ ID SEQ ID SEQ ID NO NO NO NO MOR-6032 Heavy 5 13 4 11 Light 10 14 9 12 MOR-7361 Heavy 19 27 18 25 Light 24 28 23 26 MOR-7480 Heavy 33 41 32 39 Light 38 42 37 40 MOR-7480.1 Heavy 44 49 43 47 Light 46 50 45 48 MOR-7480.2 Heavy 44 49 43 47 Light 52 54 51 53 MOR-7483 Heavy 33 41 32 39 Light 57 59 56 58 MOR-7483.1 Heavy 44 49 43 47 Light 61 63 60 62 MOR-7483.2 Heavy 44 49 43 47 Light 65 67 64 66 27 Table 2: Amino Acid Sequence of CDRs Antibody CDR Sequence SEQ ID NO MOR-6032 H-CDR1 NSYAIS 1 H-CDR2 GIIPGFGTANYAQKFQG 2 H-CDR3 RKNEEDGGFDH 3 L-CDRi SGDNLGDYYAS 6 L-CDR2 DDSNRPS 7 L-CDR3 QTWDGTLHFV 8 MOR-7361 H-CDR1 SDYYMH 15 H-CDR2 VISGSGSNTYYADSVKG 16 H-CDR3 RLYAQFEGDF 17 L-CDR1 SGDNIGSKYVS 20 L-CDR2 SDSERPS 21 L-CDR3 QSWDGSISRV 22 MOR-7480; H-CDR1 STYWIS 29 MOR-7480.1; H-CDR2 KIYPGDSYTNYSPSFQG 30 MOR-7480.2 H-CDR3 RGYGIFDY 31 L-CDRI SGDNlGDQYAH 34 L-CDR2 QDKNRPS 35 L-CDR3 ATYTGFGSLAV 36 MOR-7483; H-CDR1 STYWIS 29 MOR-74831; H-CDR2 KiYPGDSYTNYSPSFQG 30 MOR-7483.2 H-CDR3 RGYGIFDY 31 L-CDR1 SGDNIGDQYAH 34 L-CDR2 QDKNRPS 35 L-CDR3 STYTFVGFTTV 55 28 Antibodies of the present disclosure can be produced by techniques known in the art, including conventional monoclonal antibody methodology e.g., the standard somatic cell hybridization technique (See e.g., Kohler and Milstein, Nature 256:495 (1975), viral or oncogenic transformation of B lymphocytes, or recombinant antibody 5 technologies as described in detail herein below. Hybridoma production is a very well-established procedure. The common animal system for preparing hybridomas is the murine system. Immunization protocols and techniques for isolation of immunized splenocytes for fusion are known in the art. Fusion partners (e.g., murine myeloma cells) and fusion procedures are also known. 10 One well-known method that may be used for making human 4-1 BB antibodies provided by the present disclosure involves the use of a XenoMouseTM animal system. XenoMouseTM mice are engineered mouse strains that comprise large fragments of human immunoglobulin heavy chain and light chain loci and are deficient in mouse antibody production. See, e.g., Green et al., Nature Genetics 7:13-21 (1994) and 15 W02003/040170. The animal is immunized with a 4-1BB antigen. The 4-1BB antigen is isolated andfor purified 4-11BB, preferably 4-11BR. It may be a fragment of 4-11BR, such as the extracellular domain of 4-1BB, particularly a 4-1BB extracellular domain fragment comprising amino acid resides 115 - 156 of SEQ ID NO: 68. Immunization of animals can be carded out by any method known in the art. See, e.g., Harlow and Lane, 20 Antibodies: A Laboratory Manual, New York: Cold Spring Harbor Press, 1990. Methods for immunizing non-human animals such as mice, rats, sheep, goats, pigs, cattle and horses are well known in the art. See, e.g., Harlow and Lane, supra, and U.S. Pat. No. 5,994,619. The 4-1BB antigen may be administered with an adjuvant to stimulate the immune response. Exemplary adjuvants include complete or incomplete Freund's 25 adjuvant, RIBI (muramyl dipeptides) or ISCOM (immunostimulating complexes). After immunization of an animal with a 4-1BB antigen, antibody-producing immortalized cell lines are prepared from cells isolated from the immunized animal. After immunization, the animal is sacrificed and lymph node and/or splenic B cells are immortalized. Methods of immortalizing cells include, but are not limited to, transferring them with 30 oncogenes, inflecting them with the oncogenic virus cultivating them under conditions that select for immortalized cells, subjecting them to carcinogenic or mutating compounds, fusing them with an immortalized cell, e.g., a myeloma cell, and inactivating a tumor suppressor gene. See, e.g., Harlow and Lane, supra. If fusion with myeloma cells is used, the myeloma cells preferably do not secrete immunoglobulin polypeptides 29 (a non-secretory cell line). Immortalized cells are screened using 4-1BB, a portion thereof, or a cell expressing 4-1BB. 4-1BB antibody-producing cells, e.g., hybridomas, are selected, cloned and further screened for desirable characteristics, including robust growth, high antibody production and desirable antibody characteristics, as discussed 5 further below. Hybridomas can be expanded in vivo in syngeneic animals, in animals that lack an immune system, e.g., nude mice, or in cell culture in vitro. Methods of selecting, cloning and expanding hybridomas are well known to those of ordinary skill in the art. Antibodies of the disclosure can also be prepared using phage display methods. 10 Such phage display methods for isolating human antibodies are established in the art, such as the HuCAL* Libraries as described further in Example 1. See also, for example: Achim Knappik, et al: Fully Synthetic Human Combinatorial Antibody Libraries (HuCAL) Based on Modular Consensus Frameworks and CDRs Randomized with Trinucleotides. J. Mol. Biol. (2000) 296, 57-86. 15 B-2. ANTIGEN-BINDING FRAGMENTS In some other aspects, the present disclosure provides antigen-binding fragments of any of the 4-1 BB antibodies provided by the present disclosure. The antigen-binding fragment may comprise any sequences of the antibody. In some embodiments, the antigen-binding fragment comprises the amino acid sequence 20 of: (1) a light chain of a 4-iBB antibody; (2) a heavy chain of a 4-1BB antibody; (3) a variable region from the light chain of a 4-1 BB antibody; (4) a variable region from the heavy chain of a 4-1BB antibody; (5) one or more CDRs (two, three, four, five, or six CDRs) of a 4-1BB antibody; or (6) three CDRs from the light chain and three CDRs from the heavy chain of a 4-1 BB antibody. 25 In some particular embodiments, the disclosure provides an antigen-binding fragment of an antibody selected from: MOR-6032, MOR-7361, MOR-7480, MOR 7480.1, MOR-7480.2, MOR-7483, MOR-7483.1, or MOR-7483.2. In some other particular embodiments, the antigen-binding fragments of an 4 1 BB antibody include: (i) a Fab fragment, which is a monovalent fragment consisting of 30 the VL, VH, CL and CH1 domains; (ii) a F(ab') 2 fragment, which is a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; (iii) a Fd fragment consisting of the VH and CH1 domains; (iv) a Fv fragment consisting of the VL and VH domains of a single arm of an antibody; (v) a dAb fragment (Ward et al., (1989) Nature 341:544-546), which consists of a VH domain; (vi) an isolated CDR, and (vii) 30 single chain antibody (scFv), which is a polypeptide comprising a VL region of an antibody linked to a VH region of an antibody. Bird et al, (1988) Science 242:423-426 and Huston et al., (1988) Proc. Nati. Acad. Sci. USA 85:5879-5883. In some particular embodiments, the antigen-binding fragment is a Fab fragment 5 selected from the group consisting of Fab-6032, Fab-7361, Fab-7480, and Fab-7483. B-3. ANTIBODY DERIVATIVES In some further aspects, the present disclosure provides derivatives of any of the 4-1BB antibodies provided by the present disclosure. In one aspect, the antibody derivative is derived from modifications of the amino 10 acid sequences of an illustrative antibody ("parent antibody") of the disclosure while conserving the overall molecular structure of the parent antibody amino acid sequence. Amino acid sequences of any regions of the parent antibody chains may be modified, such as framework regions, CDR regions, or constant regions. Types of modifications include substitutions, insertions, deletions, or combinations thereof, of one or more 15 amino acids of the parent antibody. In some embodiments, the antibody derivative comprises an VH region that is at least 65%, at least 75%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identical to an amino acid sequence as set forth in any of SEQ ID NOs: 4,18, 32, or 43. In some other embodiments, the antibody derivative comprises an VL region that is at least 65%, at 20 least 75%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% identical to an amino acid sequence as set forth in any of SEQ ID NOs: 9, 23, 37, 45, 51, 56, 60, or 64. In some particular embodiments, the derivative comprises 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 conservative or non conservative substitutions, and/or 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 25 additions and/or deletions to an amino acid sequence as set forth in any of SEQ ID NOs: 4, 18, 32, 43, 9, 23, 37, 45, 51, 56, 60, or 64. Amino acid substitutions encompass both conservative substitutions and non conservative substitutions. The term "conservative amino acid substitution" means a replacement of one amino acid with another amino acid where the two amino acids have 30 similarity in certain physico-chemical properties such as polarity, charge, solubility, hydrophobicity, hydrophilicity, and/or the amphipathic nature of the residues involved. For example, substitutions typically may be made within each of the following groups: (a) nonpolar (hydrophobic) amino acids, such as alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, and methionine; (b) polar neutral amino acids, such 31 as glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine; (c) positively charged (basic) amino acids, such as arginine, lysine, and histidine; and (d) negatively charged (acidic) amino acids, such as aspartic acid and glutamic acid. The modifications may be made in any positions of the amino acid sequences of 5 the antibody, including the CDRs, framework regions, or constant regions. In one embodiment, the present disclosure provides an antibody derivative that contains the VH and VL CDR sequences of an illustrative antibody of this disclosure, yet contains framework sequences different from those of the illustrative antibody. Such framework sequences can be obtained from public DNA databases or published references that 10 include germline antibody gene sequences. For example, germline DNA sequences for human heavy and light chain variable region genes can be found in the Genbank database or in the "VBase" human germline sequence database (Kabat, E. A., et al., Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242 (1991); Tomlinson, I. M., et 15 al., J. Mol. Biol. 227:776-798 (1992); and Cox, J. P. L. et al., Eur. J. Immuno/. 24:827 836 (1994)). Framework sequences that may be used in constructing an antibody derivative include those that are structurally similar to the framework sequences used by illustrative antibodies of the disclosure, e.g., similar to the VH 3-23 framework sequences and/or the VL A3 or Al-13 framework sequences used by illustrative antibodies of the 20 disclosure. For example, the H-CDR1, H-CDR2, and H-CDR3 sequences, and the L CDR1, L-CDR2, and L-CDR3 sequences of an illustrative antibody can be grafted onto framework regions that have the identical sequence as that found in the germline immunoglobulin gene from which the framework sequence derive, or the CDR sequences can be grafted onto framework regions that contain one or more mutations 25 as compared to the germline sequences. In a particular embodiment, the antibody derivative is a chimeric antibody which comprises an amino acid sequence of an illustrative antibody of the disclosure. In one example, one or more CDRs from one or more illustrative human antibodies are combined with CDRs from an antibody from a non-human animal, such as mouse or rat. 30 In another example, all of the CDRs of the chimeric antibody are derived from one or more illustrative antibodies. In some particular embodiments, the chimeric antibody comprises one, two, or three CDRs from the heavy chain variable region or from the light chain variable region of an illustrative antibody. Chimeric antibodies can be generated using conventional methods known in the art.

32 Another type of modification is to mutate amino acid residues within the CDR1, CDR2 and/or CDR3 regions of the VH and/or VL chain. Site-directed mutagenesis or PCR-mediated mutagenesis can be performed to introduce the mutation(s) and the effect on antibody binding, or other functional property of interest, can be evaluated in in 5 vitro or in vivo assays known in the art. Typically, conservative substitutions are introduced. The mutations may be amino acid additions and/or deletions. Moreover, typically no more than one, two, three, four or five residues within a CDR region are altered. In some embodiments, the antibody derivative comprises 1, 2, 3, or 4 amino acid substitutions in the H-CDRs and/or in the light chain CDRs. In another embodiment, 10 the amino acid substitution is to change one or more cysteines in an antibody to another residue, such as, without limitation, alanine or serine. The cysteine may be a canonical or non-canonical cysteine. In one embodiment, the antibody derivative has 1, 2, 3, or 4 conservative amino acid substitutions in the H-CDR regions relative to the amino acid sequences of an illustrative antibody. 15 Modifications may also be made to the framework residues within the VH and/or VL regions. Typically, such framework variants are made to decrease the immunogenicity of the antibody. One approach is to "backmutate" one or more framework residues to the corresponding germline sequence. An antibody that has undergone somatic mutation may contain framework residues that differ from the 20 germline sequence from which the antibody is derived. Such residues can be identified by comparing the antibody framework sequences to the germiine sequences from which the antibody is derived. To return the framework region sequences to their germline configuration, the somatic mutations can be "backmutated" to the germline sequence by, for example, site-directed mutagenesis or PCR-mediated mutagenesis. Several of the 25 illustrative antibodies of the present disclosure underwent such "back-mutations" to certain germline sequences, as described further in Example 6. In addition, modifications may also be made within the Fc region of an illustrative antibody, typically to alter one or more functional properties of the antibody, such as serum half-life, complement fixation, Fc receptor binding, and/or antigen-dependent 30 cellular cytotoxicity. In one example, the hinge region of CH1 is modified such that the number of cysteine residues in the hinge region is altered, e.g., increased or decreased. This approach is described further in U.S. Patent No. 5,677,425. The number of cysteine residues in the hinge region of CHI is altered to, for example, facilitate assembly of the light and heavy chains or to increase or decrease the stability of the 33 antibody. In another case, the Fc hinge region of an antibody is mutated to decrease the biological half life of the antibody. Furthermore, an antibody of the disclosure may be modified to alter its potential glycosylation site or pattern. Both illustrative antibodies MOR-7480 and MOR-7483, and 5 any germlined variants thereof, and antibodies that comprise the amino acid sequences of the heavy chain variable region of MOR-7480 and MOR-7483, comprise a potential N-linked glycosylation site (NYS) at asparagine 59 in the heavy chain variable domain. IgG versions of these antibodies further comprise a second N-linked glycosylation site in the heavy chain Fc domain. More specifically, for the IgG2 version of these antibodies, 10 the Fc N-linked glycosylation site (NST) occurs at asparagine 292 in the heavy chain CH2 domain. Thus, each heavy chain can comprise 0-, 1- (at either Fab or Fc) or 2 glycan occupancy such that an antibody comprising two heavy and two light chains can comprise any combination ranging from 0-glycan occupancy (i.e., no glycosylation at any of four potential glycosylation sites) to 4-glycan occupancy (i.e., glycosylated at both 15 Fab and Fc sites in each chain). In another aspect, the present disclosure provide an derivative of an 4-1BB antibody of the disclosure that contains at least one mutation in an variable region of a light chain or heavy chain that changes the pattern of glycosylation in the variable region. Such an antibody derivative may have an increased affinity and/or a modified specificity for binding an antigen. The mutations may add a 20 novel glycosylation site in the V region, change the location of one or more V region glycosylation site(s), or remove a pre-existing V region glycosylation site. In one embodiment, the present disclosure provides a derivative of an 4-1 BB antibody having a potential N-linked glycosylation site at asparagine 59 in the heavy chain variable region, wherein the potential N-linked glycosylation site in one heavy chain variable region is 25 removed. In another embodiment, the present disclosure provides a derivative of a 4 1BB antibody having a potential N-linked glycosylation site at asparagine 59 in the heavy chain variable region, wherein the potential N-linked glycosylation site in both heavy chain variable regions is removed. Method of altering the glycosylation pattern of an antibody is known in the art, such as those described in US Patent No. 6,933,368, 30 the disclosure of which incorporated herein by reference. In another aspect, the present disclosure provides an antibody derivative that comprises a 4-1 BB antibody, or antigen-binding fragment thereof, as described herein, linked to an additional molecular entity. Examples of additional molecular entities 34 include pharmaceutical agents, peptides or proteins, detection agent or labels, and antibodies. In some embodiments, the antibody derivative comprises an antibody of the disclosure linked to a pharmaceutical agent. Examples of pharmaceutical agents include 5 cytotoxic agents or other cancer therapeutic agents, and radioactive isotopes. Specific examples of cytotoxic agents include taxol, cytochalasin B, gramicidin D, ethidium bromide, emetine, mitomycin, etoposide, tenoposide, vincristine, vinblastine, colchicin, doxorubicin, daunorubicin, dihydroxy anthracin dione, mitoxantrone, mithramycin, actinomycin D, 1-dehydrotestosterone, glucocorticoids, procaine, tetracaine, lidocaine, 10 propranolol, and puromycin and analogs or homologs thereof. Therapeutic agents also include, for example, antimetabolites (e.g., methotrexate, 6-mercaptopurine, 6 thioguanine, cytarabine, 5-fluorouracil decarbazine), alkylating agents (e.g., mechlorethamine, thioepa chiorambucil, melphalan, carmustine (BSNU) and lomustine (CCNU), cyclothosphamide, busulfan, dibromomannitol, streptozotocin, mitomycin C, 15 and cis-dichlorodiamine platinum (II) (DDP) cisplatin), anthracyclines (e.g., daunorubicin (formerly daunomycin) and doxorubicin), antibiotics (e.g., dactinomycin (formerly actinomycin), bleomycin, mithramycin, and anthramycin (AMC)), and anti-mitotic agents (e.g., vincristine and vinblastine). Examples of radioactive isotopes that can be conjugated to antibodies for use diagnostically or therapeutically include, but are not 20 limited to, iodine 131 , indium, yttrium and lutetium 1 77 . Methods for linking an antibody to a pharmaceutical agent are known in the art, such as using various linker technologies. Examples of linker types include hydrazones, thioethers, esters, disulfides and peptide-containing linkers. For further discussion of linkers and methods for linking therapeutic agents to antibodies, see also Saito et aL, Adv. Drug Deliv. Rev. 25 55:199-215 (2003); Trail, et al., Cancer Immunol. Immunother. 52:328-337 (2003); Payne, Cancer Ce/I 3:207-212 (2003) Allen, Nat. Rev. Cancer 2:750-763 (2002); Pastan, I. and Kreitman, Curr. Opin. Investing. Drugs 3:1089-1091 (2002); Senter, P.D. and Springer, C.J. (2001) Adv. Drug Deliv. Rev. 53:247-264. In a particular embodiment, the antibody derivative is a 4-1BB1 antibody multimer, 30 which is a multimeric form of a 4-1BB antibody, such as antibody dimers, timers, or higher-order multimers of monomeric antibodies. Individual monomers within an antibody multimer may be identical or different. In addition, individual antibodies within a multimer may have the same or different binding specificities. Multimerization of antibodies may be accomplished through natural aggregation of antibodies. For 35 example, some percentage of purified antibody preparations (e.g., purified IgG1 molecules) spontaneously form protein aggregates containing antibody homodimers, and other higher-order antibody multimers. Alternatively, antibody homodimers may be formed through chemical linkage techniques known in the art, such as through using 5 crosslinking agents. Suitable crosslinkers include those that are heterobifunctional, having two distinctly reactive groups separated by an appropriate spacer (such as m-maleimidobenzoyl-N-hydroxysuccinimide ester, succinimidyl 4 (maleimidomethyl)cyclohexane-1-carboxylate, and N-succinimidyl S-acethylthio-acetate) or homobifunctional (such as disuccinimidyl suberate). Such linkers are commercially 10 available from, for example, Pierce Chemical Company, Rockford, IL. Antibodies can also be made to multimerize through recombinant DNA techniques known in the art. Examples of other antibody derivatives provided by the present disclosure include single chain antibodies, diabodies, domain antibodies, nanobodies, and unibodies. A "single-chain antibody" (scFv) consists of a single polypeptide chain 15 comprising a VL domain linked to a VH domain wherein VL domain and VH domain are paired to form a monovalent molecule. Single chain antibody can be prepared according to method known in the art (see, for example, Bird et al., (1988) Science 242:423-426 and Huston et al., (1988) Proc. NatI. Acad. Sci. USA 85:5879-5883). A "diabody" consists of two chains, each chain comprising a heavy chain variable region connected 20 to a light chain variable region on the same polypeptide chain connected by a short peptide linker, wherein the two regions on the same chain do not pair with each other but with complementary domains on the other chain to form a bispecific molecule. Methods of preparing diabodies are known in the art (See, e.g., Holliger P. et al., (1993) Proc. Nati. Acad. Sci. USA 90:6444-6448, and Poijak R. J. et al, (1994) Structure 25 2:1121-1123). Domain antibodies (dAbs) are small functional binding units of antibodies, corresponding to the variable regions of either the heavy or light chains of antibodies. Domain antibodies are well expressed in bacterial, yeast, and mammalian cell systems. Further details of domain antibodies and methods of production thereof are known in the art (see, for example, U.S. Patent Nos. 6,291,158; 6,582,915; 30 6,593,081; 6,172,197; 6,696,245; European Patents 0368684 & 0616640; W005/035572, W004/101790, W004/081026, W004/058821, W004/003019 and W0031002609). Nanobodies are derived from the heavy chains of an antibody. A nanobody typically comprises a single variable domain and two constant domains (CH2 and CH3) and retains antigen-binding capacity of the original antibody. Nanobodies can 36 be prepared by methods known in the art (See e.g., U.S. Patent No. 6,765,087, U.S. Patent No. 6,838,254, WO 06/079372). Unibodies consist of one light chain and one heavy chain of a IgG4 antibody. Unibodies may be made by the removal of the hinge region of IgG4 antibodies, Further details of unibodies and methods of preparing them 5 may be found in W02007/059782. C. NUCLEIC ACIDS, VECTORS, HOST CELLS, AND RECOMBINANT METHODS OF PRODUCING 4-1BB ANTIBODIES Another aspect of the disclosure provides an isolated nucleic acid molecule that comprises a nucleotide sequence encoding an amino acid sequence of a binding 10 molecule provided by the present disclosure. The amino acid sequence encoded by the nucleotide sequence may be any portion of an antibody, such as a CDR, a sequence comprising one, two, or three CDRs, a variable region of a heavy chain, variable region of a light chain, or may be a full-length heavy chain or full length light chain. A nucleic acid of the disclosure can be, for example, DNA or RNA, and may or may not contain 15 intronic sequences. Typically, the nucleic acid is a cDNA molecule. In some embodiments, the disclosure provides an isolated nucleic acid molecule that comprises or consists of a nucleotide sequence encoding an amino acid sequence selected from the group consisting of: (1) amino acid sequence of a H-CDR3 or L CRD3 of an illustrative antibody; (2) a variable region of a heavy chain or variable region 20 of a light chain of an illustrative antibody; or (3) a full length heavy chain or full length light chain of an illustrative antibody. In other embodiments, the nucleic acid molecule comprises or consists of a nucleotide sequence that encodes an amino acid sequence as set forth in any one of SEQ ID NOs:1 - 10, 15- 24, 29 - 38, 43, 44, 45, 46, 51, 52, 55-57, 60, 61, 64, and 65. 25 In still other embodiments, the nucleic acid molecule comprises or consists of nucleotide sequence selected from the group consisting of SEQ ID NOs: 11-14, 25-28, 39-42, 47-50, 53, 54, 58, 59, 62, 63, 66, and 67. Nucleic acids of the disclosure can be obtained using any suitable molecular biology techniques. For antibodies expressed by hybridomas, cDNAs encoding the light 30 and heavy chains of the antibody made by the hybridoma can be obtained by PCR amplification or cDNA cloning techniques. For antibodies obtained from an immunoglobulin gene library (e.g., using phage display techniques), the nucleic acid encoding the antibody can be recovered from the library.

37 The isolated DNA encoding the VH region can be converted to a full-length heavy chain gene by operatively linking the VH-encoding DNA to another DNA molecule encoding heavy chain constant regions (CH1, CH2 and CH3). The sequences of human heavy chain constant region genes are known in the art (see e.g., Kabat el al. 5 (1991) Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242) and DNA fragments encompassing these regions can be obtained by standard PCR amplification. The heavy chain constant region can be an IgG1, igG2, IgG3, igG4, IgA, IgE, IgM or IgD constant region, but most preferably is an IgG1 or igG4 constant region. The IgG1 10 constant region sequence can be any of the various alleges or allotypes known to occur among different individuals, such as Gm(i), Gm(2), Gm(3), and Gm(17). These allotypes represent naturally occurring amino acid substitution in the IgG1 constant regions. For a Fab fragment heavy chain gene, the VH-encoding DNA can be operatively linked to another DNA molecule encoding only the heavy chain CH1 15 constant region. The isolated DNA encoding the VL region can be converted to a full-length light chain gene (as well as a Fab light chain gene) by operatively linking the VL-encoding DNA to another DNA molecule encoding the light chain constant region, CL. The sequences of human light chain constant region genes are known in the art (see e.g., 20 Kabat et al. (1991) Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242) and DNA fragments encompassing these regions can be obtained by standard PCR amplification. The light chain constant region can be a kappa or lambda constant region. To create a scFv gene, the VH- and VL-encoding DNA fragments are operatively 25 linked to another fragment encoding a flexible linker, e.g., encoding the amino acid sequence (Gly 4 -Ser)3, such that the VH and VL sequences can be expressed as a contiguous single-chain protein, with the VL and VH regions joined by the flexible linker (see e.g., Bird et al., Science 242:423426 (1988); Huston et al., Proc. NatL Acad. Sci. USA 85:5879-5883 (1988); and McCafferty et al., Nature 348:552-554 (1990)). 30 The present disclosure further provides a vector that comprises a nucleic acid molecule provided by the present disclosure. The nucleic acid molecule may encode a portion of a light chain or heavy chain (such as a CDR or a variable region), a full-length light or heavy chain, polypeptide that comprises a portion or full-length of a heavy or light chain, or an amino acid sequence of an antibody derivative or antigen-binding 38 fragment. In some embodiments, the vector is an expression vector useful for the expression of a binding molecule, such as an antibody or an antigen binding fragment thereof. To express a binding molecule of the disclosure, DNAs encoding partial or full 5 length light and heavy chains are inserted into expression vectors such that the DNA molecules are operatively linked to transcriptional and translational control sequences. In this context, the term "operatively linked" means that an antibody gene is ligated into a vector such that transcriptional and translational control sequences within the vector serve their intended function of regulating the transcription and translation of the DNA 10 molecule. The expression vector and expression control sequences are chosen to be compatible with the expression host cell used. The antibody light chain gene and the antibody heavy chain gene can be inserted into separate vector or, more typically, both genes are inserted into the same expression vector. The antibody genes are inserted into the expression vector by any suitable methods (e.g., ligation of complementary 15 restriction sites on the antibody gene fragment and vector, or blunt end ligation if no restriction sites are present). The light and heavy chain variable regions of the antibodies described herein can be used to create full-length antibody genes of any antibody isotype and subclass by inserting them into expression vectors already encoding heavy chain constant and light chain constant regions of the desired isotype 20 and subclass such that the VH segment is operatively linked to the CH segment(s) within the vector and the VK segment is operatively linked to the CL segment within the vector. Additionally or alternatively, the recombinant expression vector can encode a signal peptide that facilitates secretion of the antibody chain from a host cell. The antibody chain gene can be cloned into the vector such that the signal peptide is linked in-frame 25 to the amino terminus of the antibody chain gene. The signal peptide can be an immunoglobulin signal peptide or a heterologous signal peptide (i.e., a signal peptide from a non-immunoglobulin protein). In addition to the antibody chain genes, the expression vectors of the disclosure typically carry regulatory sequences that control the expression of the antibody chain 30 genes in a host cell. The term "regulatory sequence" is intended to include promoters, enhancers and other expression control elements (e.g., polyadenylation signals) that control the transcription or translation of the antibody chain genes. Such regulatory sequences are described, for example, in Goeddel (Gene Expression Technology. Methods in Enzymology 185, Academic Press, San Diego, CA (1990)). It will be 39 appreciated by those skilled in the art that the design of the expression vector, including the selection of regulatory sequences, may depend on such factors as the choice of the host cell to be transformed, the level of expression of protein desired, etc. Examples of regulatory sequences for mammalian host cell expression include viral elements that 5 direct high levels of protein expression in mammalian cells, such as promoters and/or enhancers derived from cytomegalovirus (CMV), Simian Virus 40 (SV40), adenovirus, (e.g., the adenovirus major late promoter (AdMLP) and polyoma. Alternatively, nonviral regulatory sequences may be used, such as the ubiquitin promoter or 3-globin promoter. Still further, regulatory elements composed of sequences from different sources, such 10 as the SR promoter system, which contains sequences from the SV40 early promoter and the long terminal repeat of human T cell leukemia virus type 1 (Takebe, Y. et at (1988) Mol Cell. Biol. 8:466-472). In addition to the antibody chain genes and regulatory sequences, the expression vectors may carry additional sequences, such as sequences that regulate replication of 15 the vector in host cells (e.g., origins of replication) and selectable marker genes. The selectable marker gene facilitates selection of host cells into which the vector has been introduced (see, e.g., U.S. Pat. Nos. 4,399,216, 4,634,665 and 5,179,017, all by Axel et aL). For example, typically the selectable marker gene confers resistance to drugs, such as G418, hygromycin or methotrexate, on a host cell into which the vector has 20 been introduced. Selectable marker genes include the dihydrofolate reductase (DHFR) gene (for use in dhfr- host cells with methotrexate selection/amplification) and the neo gene (for G418 selection). For expression of the light and heavy chains, the expression vector(s) encoding the heavy and light chains is transfected into a host cell by any suitable techniques. The 25 various forms of the term "transfection" are intended to encompass a wide variety of techniques commonly used for the introduction of exogenous DNA into a prokaryotic or eukaryotic host cell, e.g., electroporation, calcium-phosphate precipitation, DEAE dextran transfection and the like. Although it is possible to express the antibodies of the disclosure in either prokaryotic or eukaryotic host cells, expression of antibodies in 30 eukaryotic cells, and typically mammalian host cells, is most typical, The present disclosure further provides a host cell containing a nucleic acid molecule provided by the present disclosure. The host cell can be virtually any cell for which expression vectors are available. It may be, for example, a higher eukaryotic host cell, such as a mammalian cell, a lower eukaryotic host cell, such as a yeast cell, and 40 may be a prokaryotic cell, such as a bacterial cell. Introduction of the recombinant nucleic acid construct into the host cell can be effected by calcium phosphate transfection, DEAE, dextran mediated transfection, electroporation or phage infection. Suitable prokaryotic hosts for transformation include E. coli, Bacillus subtils, 5 Salmonella typhimurium and various species within the genera Pseudomonas, Streptomyces, and Staphylococcus. Mammalian host cells for expressing a binding molecule of the disclosure include, for example, Chinese Hamster Ovary (CHO) cells (including dhfr- CHO cells, described in Urlaub and Chasin, Proc. NatL. Acad. Sci. USA 77:42164220 (1980), used with a 10 DHFR selectable marker, e.g., as described in Kaufman and Sharp, J. Mot. Biol. 159:601-621 (1982), NSO myeloma cells, COS cells and Sp2 cells. In particular, for use with NSO myeloma or CHO cells, another expression system is the GS (glutamine synthetase) gene expression system disclosed in WO 87/04462, WO 89/01036 and EP 338,841. When expression vectors encoding antibody genes are introduced into 15 mammalian host cells, the antibodies are produced by culturing the host cells for a period of time sufficient to allow for expression of the antibody in the host cells or secretion of the antibody into the culture medium in which the host cells are grown. Antibodies can be recovered from the culture medium using any suitable protein purification methods. 20 D. COMPOSITIONS In other aspects, the present disclosure provides a composition containing a binding molecule provided by the disclosure. In one aspect, the composition is a pharmaceutical composition comprising a binding molecule and a pharmaceutically acceptable carrier. The compositions can be prepared by conventional methods known 25 in the art. The term "pharmaceutical composition" and "pharmaceutical formulation" refer to compositions comprising any of the binding molecules, any of the antibodies, any of the antigen-binding portions thereof, or a derivative thereof, as described herein together with one or more pharmaceutically acceptable excipients, diluents, or carriers as 30 required to prepare the dosage forms for the effective delivery of the binding molecule.

41 In some embodiments, the present disclosure provides a composition comprising an antibody, or an antigen-binding portion thereof, provided by the present disclosure and a pharmaceutically acceptable carrier, wherein said antibody or an antigen-binding portion comprises a variable domain comprising the CDR amino acid sequence set forth 5 in SEQ ID NO:30, and wherein said composition comprises not more than about 11%, 10%, 8%, 5%, 3%, or 2% of said antibody, or antigen-binding portion, that is glycosylated at the asparagine of said amino acid sequence compared with the total amount of antibody, or antigen-binding portion thereof, present in said composition. In another embodiment, the composition comprises at least about 2% of said antibody, or 10 antigen-binding portion, that is glycosylated at the asparagine of said amino acid sequence of SEQ ID NO:30 compared with the total amount of antibody, or antigen binding portion thereof, present in said composition. As used herein, the term "excipient" means the substances used to formulate 15 active pharmaceutical ingredients into pharmaceutical formulations; in a preferred embodiment, an excipient does not lower or interfere with the primary therapeutic effect of the active pharmaceutical ingredients. Preferably, an excipient is therapeutically inert. The term "excipient" encompasses carriers, diluents, vehicles, solubilizers, stabilizers, bulking agents, acidic or basic pH-adjusting agents and binders. Excipients can also be 20 those substances present in a pharmaceutical formulation as an indirect or unintended result of the manufacturing process. Preferably, excipients are approved for or considered to be safe for human and animal administration, i.e., GRAS substances (generally regarded as safe). GRAS substances are listed by the Food and Drug administration in the Code of Federal Regulations (CFR) at 21 CFR 182 and 21 CFR 25 184, incorporated herein by reference. The term "pharmaceutically acceptable carrier" refers to any inactive substance that is suitable for use in a formulation for the delivery of a binding molecule. A carrier may be an antiadherent, binder, coating, disintegrant, filler or diluent, preservative (such as 30 antioxidant, antibacterial, or antifungal agent), sweetener, absorption delaying agent, wetting agent, emulsifying agent, buffer, and the like. Examples of suitable pharmaceutically acceptable carriers include water, ethanol, polyols (such as glycerol, propylene glycol, polyethylene glycol, and the like) dextrose, vegetable oils ( such as olive 42 oil), saline, buffer, buffered saline, and isotonic agents such as sugars, polyalcohols, sorbitol, and sodium chloride. The term "buffering agent" refers to a pharmaceutically acceptable excipient, which stabilizes the pH of a pharmaceutical preparation. Suitable buffers are well known 5 in the art and can be found in the literature, For example, histidine buffers, glycine buffers, tris or acetate buffers, and/or the respective free acids or bases thereof, as well as mixtures of the various salts and/or acids and bases thereof can be employed. In a particular embodiment, buffers are histidine buffers, i.e. buffers having histidine, generally L-histidine, as buffering agent. A particular buffer is L-histidine/HCI buffer, 10 comprising L-histidine, L-histidine monohydrochloride, or mixtures of L-histidine and L histidine monohydrochloride. The L-histidine buffers are generally used at a concentration of about 0.05 mg/ml to about 10 mg/ml, about 0.1 mg/ml to about 5 mg/ml, or about 0.5 mg/ml to about 1 mg/mL. The L-histidine monohydrochloride buffers are generally used at a concentration of about 0.1 mg/ml to about 10 mg/ml, 15 about 1 mg/ml to about 5 mg/mi, or about 2 mg/ml to about 4 mg/ml. Regardless of the buffer used, the pH can be adjusted to a value in the range from about 4.0 to about 7.0, or about 5.0 to about 6.0, with an acid or a base known in the art, e.g. hydrochloric acid, acetic acid, phosphoric acid, sulfuric acid and citric acid, sodium hydroxide and potassium hydroxide. 20 In one aspect the present invention relates to a pharmaceutical composition comprising: 0.1 to 1 mg/ml of L-Histidine; I to 5 mg/ml of L-Histidine monohydrochloride; 50 to 100 mg/ml of Trehalose dehydrate; 0.01 to 0.1 mg/mL of Disodium EDTA dihydrate; and 0.05 to 1 mg/mL of polysorbate 80; at a pH in the range of from 4.0 to 7.0. 25 In a particular embodiment, the concentration of the binding molecule comprised in the composition according to the invention is in the range of about 10 to about 22 mg/ml. In another particular embodiment, the buffering agent comprised in the composition according to the invention is a histidine buffer, for example, a L- 43 histidine/HCI buffer, or an acetate buffer or a sodium acetate buffer. In a particular embodiment, the buffering agent is L-histidine/HCI. In a particular embodiment, the buffer is at a concentration of about 0.1 mM to about 50 mM, or from 1mM to about 25 mM. 5 The pH of a composition of the invention may be chosen from the following ranges: from 3 to 10, or from 4 to 9. In a particular embodiment, the buffering agent provides a pH of 5.0 to 6.0 or 5.5±0.3. Routine pH adjustments inside or outside of these ranges may be necessary to improve solubility or stability of the polypeptide or other components of the composition. 10 In one embodiment, the composition comprises a polysorbate, for example, polysorbate 20 or polysorbate 80. In a particular embodiment, the polysorbate 80 is at a concentration of 0,01 to 10 mg/ml, 0.5 to 5 mg/mi or 0.1 to 0.5 mg/ml. In one embodiment, at least one stabilizer comprised in the composition according to the invention is selected from the group of salts, for example, sodium 15 chloride, saccharides, trehalose dehydrate or sucrose, and amino acids, such as arginine hydrochloride. In a particular embodiment, at least one stabilizer is at a concentration of 10 to 200 mg/ml, 20 to 150 mg/mI, or 50 to 100 mg/ml. As used herein, the terms "chelating agent" generally refers to an excipient that can form at least one bond (e.g., covalent, ionic, or otherwise) to a metal ion. A 20 chelating agent is typically a multidentate ligand that can be used in selected liquid compositions as a stabilizer to complex with species, which might promote instability. Often, compounds that can act as a cheating agent will have electron-rich functional groups. Suitable electron-rich functional groups include carboxylic acid groups, hydroxy groups and amino groups. Arrangement of these groups in aminopolycarboxylic acids, 25 hydroxypolycarboxylic acids, hydroxyaminocarboxylic acids, and the like, result in moieties that have the capacity to bind metal. However, the present invention is not intended to be limited to chelating agents that enhance antibody stability primarily by the cheating agent's ability to form bonds with a metal ion, Therefore, the present invention is not intended to be limited by the 30 specific mechanism by which the cheating agent stabilizes the compositions of the 44 present invention and the excipients termed chelating agents herein may achieve their antibody stability enhancing properties primarily through mechanisms that are altogether unrelated to the cheating agents ability to form bonds with a metal ion. Chelating agents that are suitable for use in the present invention, include, but 5 are not limited to, aminopolycarboxylic acids, hydroxyaminocarboxylic acids, N substituted glycines, 2-(2-amino-2-oxoethyl) aminoethane sulfonic acid (BES), deferoxamine (DEF), citric acid, niacinamide, and desoxycholates. Examples of suitable aminopolycarboxylic acids include ethylenediaminetetraacetic acid (EDTA), diethylenetriamine pentaacetic acid 5 (DTPA), nitrilotriacetic acid (NTA), N-2-acetamido 10 2-iminodiacetic acid (ADA), bis(aminoethyl)glycolether, N,N,N',N'-tetraacetic acid (EGTA), trans-diaminocyclohexane tetraaceic acid (DCTA), glutamic acid, and aspartic acid. Examples of suitable hydroxyaminocarboxylic acids include N hydroxyethyliminodiacetic acid (HIMDA), N,N-bis-hydroxyethylglycine (bicine) and N (trishydroxymethylmethy) 10 glycine (tricine). An example of a suitable N-substituted 15 glycine is glycylglycine. An example of a suitable desoxycholate is sodium desoxycholate. Chelating agents used in the invention can be present, where possible, as the free acid or free base form of the compound (e.g., referred to interchangeably herein as "EDTA" or "edetate") or as a corresponding salt form (e.g., the corresponding acid 20 addition salt or base addition salt, such as disodium edetate). Suitable acid addition salts, e.g., include alkali metal salts (e.g., sodium or potassium salts), alkaline earth metal salts (e.g., calcium), and salts can be prepared using other weakly bound metal ions. As is known in the art, the nature of the salt and the number of charges to be neutralized will depend on the number of carboxyl groups present and the pH at which 25 the stabilizing chelating agent is supplied. As is also known in the art, chelating agents have varying strengths with which particular target ions are bound. By way of further illustration, suitable salts of EDTA include dipotassium edetate, disodium edetate, edetate calcium disodium, sodium edetate, trisodium edetate, and potassium edetate; and a suitable salt of deferoxamine (DEF) is deferoxamine mesylate (DFM). 30 Chelating agents used in the invention can be present as an anhydrous, solvated or hydrated form of the compound or corresponding salt. Where the chelating agent is in a solvated or hydrated form, it can be present in varying states of solvation or hydration 45 (including, e.g., anhydrous, hydrated, dihydrated, and trihydrated forms). By way of further illustration, a suitable hydrate of EDTA is disodium EDTA dihydrate. In one embodiment, the disodium EDTA dihydrate is at a concentration of 0.001 to 5 mg/mi, 0.01 to 2 mg/ml, and 0.02 to 0.5 mg/mI. In another embodiment, the chelating agent 5 can reduce or prevent aggregation of the antibodies in the compositions described herein. Such chelating agents can reduce or prevent degradation of an antibody that is formulated without the protection of a chelating agent. Pharmaceutical compositions can generally be tailored to the specific intended route of administration. Multiple techniques of administering a compound exist in the art 10 including, but not limited to, oral, aerosol, parenteral, and topical administration. The compositions may be in any suitable forms, such as liquid, semi-solid, and solid dosage forms. Examples of liquid dosage forms include solution (e.g., injectable and infusible solutions), microemulsion, liposome, dispersion, or suspension. Examples of solid dosage forms include tablet, pill, capsule, microcapsule, and powder. A particular form 15 of the composition suitable for delivering a binding molecule is a sterile liquid, such as a solution, suspension, or dispersion, for injection or infusion. Sterile solutions can be prepared by incorporating the antibody in the required amount in an appropriate carrier, followed by sterilization microfiltration. Generally, dispersions are prepared by incorporating the antibody into a sterile vehicle that contains a basic dispersion medium 20 and other carriers. In the case of sterile powders for the preparation of sterile liquid, methods of preparation include vacuum drying and freeze-drying (lyophilization) to yield a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof. The various dosage forms of the compositions can be prepared by conventional techniques known in the art. 25 The relative amount of a binding molecule included in the composition will vary depending upon a number of factors, such as the specific binding molecule and carriers used, dosage form, and desired release and pharmacodynamic characteristics. In one embodiment, the composition comprises a binding molecule wherein said binding molecule concentration is between 1 to 100 mg/ml, between 5 to 50 mg/ml, or between 30 10 to 22 mg/mI. The amount of a binding molecule in a single dosage form will generally be that amount which produces a therapeutic effect, but may also be a lesser amount. Generally, this amount will range from about 0.01 percent to about 99 percent, 46 from about 0.1 percent to about 70 percent, or from about 1 percent to about 30 percent relative to the total weight of the dosage form. In addition to the binding molecule, one or more additional therapeutic agents may be included in the composition. Examples of additional therapeutic agents are described 5 herein below. The suitable amount of the additional therapeutic agent to be included in the composition can be readily selected by a person skilled in the art, and will vary depending on a number of factors, such as the particular agent and carriers used, dosage form, and desired release and pharmacodynamic characteristics. The amount of the additional therapeutic agent included in a single dosage form will generally be that amount of the 10 agent which produces a therapeutic effect, but may be a lesser amount as well. E. USE OF THE BINDING MOLECULES AND PHARMACEUTICAL COMPOSITIONS Binding molecules and pharmaceutical compositions provided by the present disclosure are useful for therapeutic, diagnostic, or other purposes, such as enhancing 15 an immune response, treating cancer, enhancing efficacy of other cancer therapy, enhancing vaccine efficacy, or treating autoimmune diseases. Thus, in other aspects, the present disclosure provides methods of using the binding molecules or pharmaceutical compositions. In one aspect, the present disclosure provides a method of treating a disorder in a mammal, which comprises administering to the mammal in 20 need of treatment a therapeutically effective amount of a binding molecule provided by the disclosure. The binding molecule may be a 4-1BB agonist or antagonist. In some embodiments, the binding molecule is a 4-lBB agonist. In some embodiments, the mammal is a human. In some embodiments, the disorder is a cancer. A variety of cancers where 4 25 1 BB is implicated, whether malignant or benign and whether primary or secondary, may be treated or prevented with a method provided by the disclosure. Examples of such cancers include lung cancers such as bronchogenic carcinoma (e.g., squamous cell carcinoma, small cell carcinoma, large cell carcinoma, and adenocarcinoma), alveolar cell carcinoma, bronchial adenoma, chondromatous hamartoma (noncancerous), and 30 sarcoma (cancerous); heart cancer such as myxoma, fibromas, and rhabdomyomas; bone cancers such as osteochondromas, condromas, chondroblastomas, chondromyxoid fibromas, osteoid osteomas, giant cell tumors, chondrosarcoma, multiple myeloma, osteosarcoma, fibrosarcomas, malignant fibrous histiocytomas, Ewing's tumor (Ewing's sarcoma), and reticulum cell sarcoma; brain cancer such as 47 gliomas (e.g., glioblastoma multiforme), anaplastic astrocytomas, astrocytomas, oligodendrogliomas, medulloblastomas, chordoma, Schwannomas, ependymomas, meningiomas, pituitary adenoma, pinealoma, osteomas, hemangioblastomas, craniopharyngiomas, chordomas, germinomas, teratomas, dermoid cysts, and 5 angiomas; cancers in digestive system such as leiomyoma, epidermoid carcinoma, adenocarcinoma, leiomyosarcoma, stomach adenocarcinomas, intestinal lipomas, intestinal neurofibromas, intestinal fibromas, polyps in large intestine, and colorectal cancers; liver cancers such as hepatocellular adenomas, hemangioma, hepatocellular carcinoma, fibrolamellar carcinoma, cholangiocarcinoma, hepatoblastoma, and 10 angiosarcoma; kidney cancers such as kidney adenocarcinoma, renal cell carcinoma, hypernephroma, and transitional cell carcinoma of the renal pelvis; bladder cancers; hematological cancers such as acute lymphocytic (lymphoblastic) leukemia, acute myeloid myelocyticc, myelogenous, myeloblastic, myelomonocytic) leukemia, chronic lymphocytic leukemia (e.g., Sezary syndrome and hairy cell leukemia), chronic 15 myelocytic (myeloid, myelogenous, granulocytic) leukemia, Hodgkin's lymphoma, non Hodgkin's lymphoma, B cell lymphoma, mycosis fungoides, and myeloproliferative disorders (including myeloproliferative disorders such as polycythemia vera, myelofibrosis, thrombocythemia, and chronic myelocytic leukemia); skin cancers such as basal cell carcinoma, squamous cell carcinoma, melanoma, Kaposi's sarcoma, and 20 Paget's disease; head and neck cancers; eye-related cancers such as retinoblastoma and intraoccular melanocarcinoma; male reproductive system cancers such as benign prostatic hyperplasia, prostate cancer, and testicular cancers (e.g., seminoma, teratoma, embryonal carcinoma, and choriocarcinoma); breast cancer; female reproductive system cancers such as uterine cancer (endometrial carcinoma), cervical 25 cancer (cervical carcinoma), cancer of the ovaries (ovarian carcinoma), vulvar carcinoma, vaginal carcinoma, fallopian tube cancer, and hydatidiform mole; thyroid cancer (including papillary, follicular, anaplastic, or medullary cancer); pheochromocytomas (adrenal gland); noncancerous growths of the parathyroid glands; pancreatic cancers; and hematological cancers such as leukemias, myelomas, non 30 Hodgkin's lymphomas, and Hodgkin's lymphomas. In some other embodiments, the disorder is an autoimmune disease. Examples of autoimmune diseases that may be treated with the binding molecules include autoimmune encephalomyelitis, lupus erythematosus, and rheumatoid arthritis. The 48 binding molecule may also be used to treat inflammation (such as allergic asthma) and chronic graft-versus-host disease, In another aspect, the present disclosure provides a method of enhancing an immune response in a mammal, which comprises administering to the mammal a 5 therapeutically effective amount of a binding molecule provided by the disclosure. In some embodiments, the binding molecule is a 4-11BB3 antibody or antigen-binding fragment thereof and the mammal is a human. In a further embodiment, the binding molecule is 4-1BB agonist antibody or an antigen-binding fragment thereof. The term "enhancing immune response" or its grammatical variations, means stimulating, 10 evoking, increasing, improving, or augmenting any response of a mammal's immune system. The immune response may be a cellular response (i.e. cell-mediated, such as cytotoxic T lymphocyte mediated) or a humoral response (i.e. antibody mediated response), and may be a primary or secondary immune response. Examples of enhancement of immune response include increased CD4+ helper T cell activity and 15 generation of cytolytic T cells. The enhancement of immune response can be assessed using a number of in vitro or in vivo measurements known to those skilled in the art, including, but not limited to, cytotoxic T lymphocyte assays, release of cytokines (for example IL-2 production), regression of tumors, survival of tumor bearing animals, antibody production, immune cell proliferation, expression of cell surface markers, and 20 cytotoxicity. Typically, methods of the disclosure enhance the immune response by a mammal when compared to the immune response by an untreated mammal or a mammal not treated using the claimed methods. In one embodiment, the binding molecule is used to enhance the immune response of a human to a microbal pathogen (such as a virus). In another embodiment, the binding molecule is used to enhance the 25 immune response of a human to a vaccine. The binding molecule may be a 4-1BB agonist or antagonist. In some embodiments, the binding molecule is a 4-1 BB agonist. In one embodiment, the method enhances a cellular immune response, particularly a cytotoxic T cell response. In another embodiment, the cellular immune response is a T helper cell response. In still another embodiment, the immune response is a cytokine 30 production, particularly IL-2 production. The binding molecule may be used to enhance the immune response of a human to a microbial pathogen (such as a virus) or to a vaccine. The binding molecule may be a 4-1BB agonist or antagonist. In some embodiments, the binding molecule is a 4-1 BB agonist.

49 In practicing the therapeutic methods, the binding molecules may be administered alone as monotherapy, or administered in combination with one or more additional therapeutic agents or therapies. Thus, in another aspect, the present disclosure provides a combination therapy, which comprises a binding molecule in 5 combination with one or more additional therapies or therapeutic agents for separate, sequential or simultaneous administration. The term "additional therapy" refers to a therapy which does not employ a binding molecule provided by the disclosure as a therapeutic agent. The term "additional therapeutic agent" refers to any therapeutic agent other than a binding molecule provided by the disclosure. In one particular 10 aspect, the present disclosure provides a combination therapy for treating cancer in a mammal, which comprises administering to the mammal a therapeutically effective amount of a binding molecule provided by the disclosure in combination with one or more additional therapeutic agents. In a further embodiment, the mammal is a human. A wide variety of cancer therapeutic agents may be used in combination with a 15 binding molecule provided by the present disclosure. One of ordinary skill in the art will recognize the presence and development of other cancer therapies which can be used in combination with the methods and binding molecules of the present disclosure, and will not be restricted to those forms of therapy set forth herein. Examples of categories of additional therapeutic agents that may be used in the combination therapy for treating 20 cancer include (1) chemotherapeutic agents, (2) immunotherapeutic agents, and (3) hormone therapeutic agents. The term "chemotherapeutic agent" refers to a chemical or biological substance that can cause death of cancer cells, or interfere with growth, division, repair, and/or function of cancer cells. Examples of chemotherapeutic agents include those that are 25 disclosed in WO 2006/088639, WO 2006/129163, and US 20060153808, the disclosures of which are incorporated herein by reference. Examples of particular chemotherapeutic agents include: (1) alkylating agents, such as chlorambucil (LEUKERAN), moyclophosphamide (CYTOXAN), ifosfamide (IFEX), mechlorethamine hydrochloride (MUSTARGEN), thiotepa (THIOPLEX), streptozotocin (ZANOSAR), 30 carmustine (BICNU, GLIADEL WAFER), lomustine (CEENU), and dacarbazine (DTIC DOME); (2) alkaloids or plant vinca alkaloids, including cytotoxic antibiotics, such as doxorubicin (ADRIAMYCIN), epirubicin (ELLENCE, PHARMORUBICIN), daunorubicin (CERUBIDINE, DAUNOXOME), nemorubicin, idarubicin (IDAMYCIN PFS, ZAVEDOS), mitoxantrone (DHAD, NOVANTRONE). dactinomycin (actinomycin D, COSMEGEN), 50 plicamycin (MITHRACIN), mitomycin (MUTAMYCIN), and bleomycin (BLENOXANE), vinorelbine tartrate (NAVELBINE)), vinblastine (VELBAN), vincristine (ONCOVIN), and vindesine (ELDISINE); (3) antimetabolites, such as capecitabine (XELODA), cytarabine (CYTOSAR-U), fludarabine (FLUDARA), gemcitabine (GEMZAR), hydroxyurea 5 (HYDRA), methotrexate (FOLEX, MEXATE, TREXALL), nelarabine (ARRANON), trimetrexate (NEUTREXIN), and pemetrexed (ALIMTA); (4) Pyrimidine antagonists, such as 5-fluorouracil (5-FU); capecitabine (XELODA), raltitrexed (TOMUDEX), tegafur uracil (UFTORAL), and gemcitabine (GEMZAR); (5) taxanes, such as docetaxel (TAXOTERE), paclitaxel (TAXOL); (6) platinum drugs, such as cisplatin (PLATINOL) 10 and carboplatin (PARAPLATIN), and oxaliplatin (ELOXATIN); (7) topoisomerase inhibitors, such as irinotecan (CAMPTOSAR), topotecan (HYCAMTIN), etoposide (ETOPOPHOS, VEPESSID, TOPOSAR), and teniposide (VUMON); (8) epipodophyllotoxins (podophyllotoxin derivatives), such as etoposide (ETOPOPHOS, VEPESSID, TOPOSAR); (9) folic acid derivatives, such as leucovorin 15 (WELLCOVORIN); (10) nitrosoureas, such as carmustine (BiCNU), lomustine (CeeNU); (11) inhibitors of receptor tyrosine kinase, including epidermal growth factor receptor (EGFR), vascular endothelial growth factor (VEGF), insulin receptor, insulin-like growth factor receptor (IGFR), hepatocyte growth factor receptor (HGFR), and platelet-derived growth factor receptor (PDGFR), such as gefitinib (IRESSA), erlotinib (TARCEVA), 20 bortezomib (VELCADE), imatinib mesylate (GLEEVEC), genefitinib, lapatinib, sorafenib, thalidomide, sunitinib (SUTENT), axitinib, rituximab (RITUXAN, MABTHERA),, trastuzumab (HERCEPTIN), cetuximab (ERBITUX), bevacizumab (AVASTIN), and ranibizumab (LUCENTIS), lym-1 (ONCOLYM), antibodies to insulin-like growth factor -1 receptor (IGF-1R) that are disclosed in W02002/053596); (12) angiogenesis inhibitors, 25 such as bevacizumab (AVASTIN ), suramin (GERMANIN), angiostatin, SU5416, thalidomide, and matrix metalloproteinase inhibitors (such as batimastat and marimastat), and those that are disclosed in W02002055106; and (13) proteasome inhibitors, such as bortezomib (VELCADE). The term immunotherapeutic agents" refers to a chemical or biological 30 substance that can enhance an immune response of a mammal. Examples of immunotherapeutic agents include: bacillus Calmette-Guerin (BCG); cytokines such as interferons; vaccines such as MyVax personalized immunotherapy, Onyvax-P, Oncophage, GRNVAC1, FavId, Provenge, GVAX, Lovaxin C, BiovaxlD, GMXX, and NeuVax; and antibodies such as alemtuzumab (CAMPATH), bevacizumab (AVASTIN), 51 cetuximab (ERBITUX), gemtuzunab ozogamicin (MYLOTARG), ibritumomab tiuxetan (ZEVALIN), panitumumab (VECTIBIX), rituximab (RITUXAN, MABTHERA), trastuzumab (HERCEPTIN), tositumomab (BEXXAR), ipilimumab (YERVOY) tremelimumab, CAT 3888, agonist antibodies to OX40 receptor (such as those disclosed in 5 W02009/079335), agonist antibodies to CD40 receptor (such as those disclosed in W02003/040170, and TLR-9 agonists (such as those disclosed in W02003/015711, W02004/016805, and W02009/022215). The term "hormone therapeutic agent" refers to a chemical or biological substance that inhibits or eliminates the production of a hormone, or inhibits or 10 counteracts the effect of a hormone on the growth and/or survival of cancerous cells. Examples of such agents suitable for the methods herein include those that are disclosed in US20070117809. Examples of particular hormone therapeutic agents include tamoxifen (NOLVADEX), toremifene (Fareston), fulvestrant (FASLODEX), anastrozole (ARIMIDEX), exemestane (AROMASIN), letrozole (FEMARA), megestrol 15 acetate (MEGACE), goserelin (ZOLADEX), and leuprolide (LUPRON). The binding molecules of this disclosure may also be used in combination with non-drug hormone therapies such as (1) surgical methods that remove all or part of the organs or glands which participate in the production of the hormone, such as the ovaries, the testicles, the adrenal gland, and the pituitary gland, and (2) radiation treatment, in which the organs 20 or glands of the patient are subjected to radiation in an amount sufficient to inhibit or eliminate the production of the targeted hormone. The combination therapy for treating cancer also encompasses the combination of a binding molecule with surgery to remove a tumor. The binding molecule may be administered to the mammal before, during, or after the surgery. 25 The combination therapy for treating cancer also encompasses combination of a binding molecule with radiation therapy, such as ionizing (electromagnetic) radiotherapy (e.g., X-rays or gamma rays) and particle beam radiation therapy (e.g., high linear energy radiation). The source of radiation can be external or internal to the mammal. The binding molecule may be administered to the mammal before, during, or after the 30 radiation therapy. The binding molecules and compositions provided by the present disclosure can be administered via any suitable enteral route or parenteral route of administration. The term "enteral route" of administration refers to the administration via any part of the gastrointestinal tract. Examples of enteral routes include oral, mucosal, buccal, and 52 rectal route, or intragastric route. "Parenteral route" of administration refers to a route of administration other than enteral route, Examples of parenteral routes of administration include intravenous, intramuscular, intradermal, intraperitoneal, intratumor, intravesical, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, transtracheal, 5 intraarticular, subcapsular, subarachnoid, intraspinal, epidural and intrasternal, subcutaneous, or topical administration. The antibodies and compositions of the disclosure can be administered using any suitable method, such as by oral ingestion, nasogastric tube, gastrostomy tube, injection, infusion, implantable infusion pump, and osmotic pump. The suitable route and method of administration may vary depending on 10 a number of factors such as the specific antibody being used, the rate of absorption desired, specific formulation or dosage form used, type or severity of the disorder being treated, the specific site of action, and conditions of the patient, and can be readily selected by a person skilled in the art The term "therapeutically effective amount' of a binding molecule refers to an 15 amount that is effective for an intended therapeutic purpose. For example, in the context of enhancing an immune response, a "therapeutically effective amount" is any amount that is effective in stimulating, evoking, increasing, improving, or augmenting any response of a mammal's immune system. In the context of treating a disease, a "therapeutically effective amount" is any amount that is sufficient to cause any desirable 20 or beneficial effect in the mammal being treated. Specifically, in the treatment of cancer, examples of desirable or beneficial effects include inhibition of further growth or spread of cancer cells, death of cancer cells, inhibition of reoccurrence of cancer, reduction of pain associated with the cancer, or improved survival of the mammal. The therapeutically effective amount of a 4-1 BB antibody usually ranges from about 0.001 to 25 about 500 mg/kg, and more usually about 0.01 to about 100 mg/kg, of the body weight of the mammal. For example, the amount can be about 0.3 mg/kg, 1 mg/kg, 3 mg/kg, 5 mg/kg, 10 mg/kg, 50 mg/kg, or 100 mg/kg of body weight of the mammal. In some embodiments, the therapeutically effective amount of a 4-1BB antibody is in the range of about 0.01 - 30 mg/kg of body weight of the mammal. In some other embodiments, the 30 therapeutically effective amount of a 4-1BB antibody is in the range of about 0.05 - 15 mg/kg of body weight of the mammal. The precise dosage level to be administered can be readily determined by a person skilled in the art and will depend on a number of factors, such as the type, and severity of the disorder to be treated, the particular binding molecule employed, the route of administration, the time of administration, the 53 duration of the treatment, the particular additional therapy employed, the age, sex, weight, condition, general health and prior medical history of the patient being treated, and like factors well known in the medical arts. A binding molecule or composition is usually administered on multiple occasions. 5 Intervals between single doses can be, for example, weekly, monthly, every three months or yearly. An exemplary treatment regimen entails administration once per week, once every two weeks, once every three weeks, once every four weeks, once a month, once every three months or once every three to six months. Typical dosage regimens for a 4-1BB antibody include 1 mg/kg body weight or 3 mg/kg body weight via 10 intravenous administration, using one of the following dosing schedules: (i) every four weeks for six dosages, then every three months (ii) every three weeks; (iii) 3 mg/kg body weight once followed by 1 mg/kg body weight every three weeks. The present disclosure is further illustrated by the following examples which should not be construed as further limiting. The contents of all figures and all 15 references, patents and published patent applications cited throughout this disclosure are expressly incorporated herein by reference in their entirety.

54 EXAMPLES EXAMPLE 1: GENERATION OF FAB FRAGMENTS THAT BIND TO 4-1BB Certain antibodies provided by the present invention were originally generated 5 from Fabs that bind to human 4-1BB. The Fabs were selected from a phage display library, the MorphoSys HuCAL GOLD* phagemid library, following alternating panning on 4-1 BB FC and cells expressing human 4-1 BB. These Fabs include those that are designated as "Fab-6032," "Fab-7361," "Fab-7480," and "Fab-7483." 4-1BB antibodies MOR-6032, MOR-7361, MOR-7480, and MOR-7483 disclosed in this application were 10 generated from "Fab-6032," "Fab-7361," "Fab-7480," and "Fab-7483, respectively. The amino acid sequence of the light chain variable region and heavy chain variable region of a given Fab are identical to the amino acid sequence of the light chain variable region and heavy chain variable region, respectively, of an illustrative antibody the designation of which shares the same numerical number with the designation of the Fab. For 15 example, Fab-7480 and antibody MOR-7480 have identical amino acid sequences for their light chain variable region and heavy chain variable region, respectively. The phagemid library is based on the HuCAL* concept (Knappik et al., 2000, J. Mol. Biol. 296(1):57-86) and employs the CysDisplay M technology for displaying the Fab on the phage surface (Lbhning, WO 01/05950). HuCAL GOLD* provides the option 20 of performing selections with six single sub-libraries each comprising one VH (VH1, VH2, VH3, VH4, VH5, VH6) master gene combined with all seven VL master genes or performing selections using combined phage pools. Phage for the 1st round of panning were prepared by Hyperphage (M13KO7ApIII, obtained from Progen, Heidelberg, Germany). HuCAL GOLD* is described in detail in Christine Rothe, et. al, J. Mol. Biol. 25 (2008)376,1182-1200. Solid phase panning was performed using recombinant human 4-1BB-Fc (R&D Systems, Cat. No. 838-4B; Minneapolis, MN), 55 EXAMPLE 2. CHARACTERIZATIONS OF FABS The characterizations of the four Fabs described in example I were determined in the assays described below using the monovalent Fab-format comprising a Fab having a Flag/His-Tag. 5 2A. Affinity Determined with Solution Equilibrium Titration (SET) Method The affinity (as expressed as Ko) of the four Fabs was determined using the SET method using an instrumentation from Meso Scale Discovery ("MSD"). Monomer fractions of antibody protein were used (at least 90% monomer content, analyzed by analytical SEC Superdex75 (Amersham Pharmacia) for Fab, or Tosoh G3000SWXL 10 (Tosoh Bioscience) for IgG, respectively). Affinity determination in solution was basically performed as described in the literature (Friguet et al. 1985). In order to improve the sensitivity and accuracy of the SET method, it was transferred from classical ELISA to ECL based technology (Haenel et al. 2005). 15 1 mg/ml goat-anti-human (Fab) 2 fragment specific antibodies (Dianova) were labeled with MSD Sulfo-TAGT" NHS-Ester (Meso Scale Discovery, Gaithersburg, MD, USA) according to manufacturer's instructions. The experiments were carried out in polypropylene microtiter plates and PBS pH 7.4 with 0.5% BSA and 0.02% Tween 20 as assay buffer. Unlabeled human 4-1BB was 20 diluted in a 2" series, starting with a concentration at least 10 times higher than the expected KD. Wells without antigen were used to determine B. values; wells with assay buffer were used to determine background. After addition of, e.g., 30 pM Fab (final concentration in 60 pL final volume), the mixture was incubated overnight at room temperature. The Fab concentration applied was similar to or below the expected KD. 25 Standard MSD plates were coated with 0.05 pg/ml human 4-1BB in PBS (30 pL/well), incubated overnight, and blocked with 3% BSA in PBS for 1 hour. After washing the plate with assay buffer, the equilibrated samples were transferred to those plates (30 pL per well) and incubated for 20 minutes. After washing, 30 pLIwell of the MSD Sulfo-tag labeled detection antibody (goat anti-human (Fab) 2 ) in a final dilution of 30 1:1600 was added to the MSD plate and incubated for 30 min on an Eppendorf shaker (700 rpm). After washing the plate and adding 30 pL/well MSD Read Buffer T with surfactant, electrochemiluminescence signals were detected using a Sector Imager 6000 (Meso Scale Discovery, Gaithersburg, MD, USA).

56 The data were evaluated with XLfit (IDBS) software applying customized fitting models. For KD determination of Fab molecules the following fit model was used (Haenel et al., 2005) and modified according to Abraham et al. (1996, J. Molec. Recog. 9(5 6):456-461): 5 = -. r - Fab]-,+x+K .([Fab],+x+K,)f-4x[Fab]J, [Fabjt: Applied total Fab concentration x: Applied total soluble antigen concentration (binding sites) Bm : Maximal signal of Fab without antigen 10 Ko: Affinity Results are presented in Table 3. 2B. Biacore KD Determination on Directly Coated Antigen 15 For K 0 determination, monomeric Fab fractions (at least 90% monomer content, analyzed by analytical SEC; Superdex75, Amersham Pharmacia) were used as analyte. Binding to immobilized antigen was analyzed using a BIAcore3OO0 instrument (Biacore, Sweden). The kinetic rate constants k 0 n and kff were determined with serial dilutions of the 20 respective Fab binding to covalently immobilized antigen CD137/ HUMAN 4-1BB using the Biacore 3000 instrument (Biacore, Uppsala, Sweden). For covalent antigen immobilization standard EDC-NHS amine coupling chemistry was used. Kinetic measurements were done in HBS-EP (10mM HEPES; pH 7.4; 150mM NaCl; 3mM EDTA; Tween20 0.005%) at a flow rate of 20 pl/minute using Fab concentrations 25 ranging from about 16 to 500 nM. The injection time for each concentration was 1 minute, followed by at least 3 minutes dissociation phase. For regeneration, one or more 5 pl injections of Glycine/HCI pH2 were used. For Ko estimation of whole igG molecules, IgGs were injected as samples on a F1 sensor chip with a low density of covalently immobilized human 4-1 BB (approx. 130 30 RU) using a 2" serial dilution with concentrations ranging from 16 to 500 nM. Sensorgrams were evaluated using a bivalent fit model a qualitatively compared to rank the corresponding Ko values.

57 All sensorgrams were fitted using BIA evaluation software 3.1 (Biacore). Results are presented in Table 3. 2C. Binding of Fabs in ELISA Assay 5 The binding of the four Fabs were determined using standard ELISA techniques on directly coated human 4-1BB/Fc. Results are presented in Table 3. 20. Binding of Fabs in FACS assay The binding of the four Fabs was determined using standard FACS assay 10 techniques on HEK293 cells stably transfected and expressing human 4-1BB as well as 300.19 (murine B-cell line) negative control cells. The results are presented in Table 3. Table 3. Binding Properties of Fabs BlAcore SET Affinity ELISA Assay FACS Assay Fab Affinity Ko [pM] EC50 [nM] EC50 [nM] Ko [nM] Fab-6032 66 Not 1.0 270 measured Fab-7361 118 Not 0.6 105 measured Fab-7480 0.5 46 0.7 0.9 Fab-7483 0.7 43 0.6 8.9 15 EXAMPLE 3: CHARACTERIZATION OF IgGs Several Fabs obtained from the panning as described herein, including Fab 6032, Fab-7361, Fab-7480, and Fab-7483, were selected for conversion into full length antibodies in IgG1 and IgG4 formats for further characterizations as described in this example. The four illustrative antibodies identified in this example, le., MOR-6032, 20 MOR-7361, MOR-7480, and MOR-7483, were converted from Fab-6032, Fab- 7361, Fab-7480, and Fab-7483, respectively. The antibodies in IgG format were expressed and purified, and then characterized in ELISA, FACS, and luciferase reporter gene assays.

58 3A. Conversion to lqG In order to express full length IgG, variable domain fragments of heavy (VH) and light chains (VL) were subeloned from Fab expression vectors into appropriate pMorph*_hlgG vectors for human IgG1 and human IgG4. 5 3B. Transient Expression and Purification of Human loG Transient expression of full length human igG was performed in HKB1 1 cells, which were transfected with IgG heavy and light chain expression vectors at a 1:1 ratio. Cell culture supernatant was harvested after transfection and upscaled to 3-fold transfection volume, respectively. Supernatant was cleared by centrifugation and 10 filtration, and then subjected to standard protein A affinity chromatography (MabSelect SURE, GE Healthcare). Proteins were elated and neutralized. Further downstream processing involved buffer exchange and sterile filtration. Protein concentrations were determined by UV-spectrophotometry. Purity of IgG was analyzed under denaturing, reducing and denaturing, non-reducing conditions in SDS-PAGE or by using Agilent 15 BioAnalyzer. HP-SEC was performed to analyze IgG preparations in native state. 3C. Characterization of iGs in ELISA Assay IgGs were used for ELISA binding characterization on human 4-1BB/Fc and mouse 4-1BB/Fc in a direct coated setup. Table 4 below sets out the ELISA binding results for antibodies MOR-6032, MOR-7361, MOR-7480, and MOR-7483, all in IgG1 20 format. Table 4. Binding of IgGis in ELISA Assay Antibody Human 4-1BB/Fc Mouse 4-1BB/Fc ECO [nM] MOR-6032 0,2 MOR-7361 0.3 MOR-7480 0.7 (+) MOR-7483 0.9 3D. Binding Selectivity of Antibodies (FACS Assay) 25 The selectivity of antibodies for 4-1 BB was assessed against extracellular domain protein of 4-1BB and other members of the TNFR superfamily. These receptors included CD40 (TNFRSF5) and OX-40 (CD134, TNFRSF4). IgGs were used for FACS binding characterization on negative control HEK293 cells, as well as HEK293T-h4-1 BB 59 cells stably transfected and expressing human 4-1BB, and 300.19 stably transfected cells expressing OX-40, and 300.19 cells stably transfected and expressing CD40. The FACS binding results for antibodies MOR-6032, MOR-7361, MOR-7480, and MOR 7483, all in IgG1 format, are presented in Table 5. No significant binding to OX-40 or 5 CD40 was observed at concentrations up to 1000 nM, demonstrating that the antibodies are at least 100-fold more selective for 4-1BB versus other related family members tested. Table 5. Binding Selectivity of Antibodies (IgG1) in FACS Assay Antibody HEK293T parental 300.19 300.19 300.19 4-1BB HEK293 OX40 CD40 parental EC, [nM] MOR-6032 0,6 - MOR-7361 0.8 - MOR-7480 0.6 MOR-7483 0.5 (+) 10 3E. Characterization of laGs in Luciferase Reporter Gene Assay IgGs were also characterized for binding in a luciferase reporter gene assay using HEK293T-h4-IBB cells in a plate bound assay, a soluble binding assay, and cross-linked binding assay. Table 6 sets out the results of the luciferase reporter gene 15 assay for antibodies MOR-6032, MOR-7361, MOR-7480, and MOR-7483, all in IgG1 format. Table 6. Characterization of IgG1 in Luciferase Reporter Gene Assay Luciferase reporter gene assay of IgGs Antibody plate-bound soluble cross-linked MOR-6032 +-+ MOR-7361 + MOR-7480 + MOR-7483 + 20 EXAMPLE 4. STRUCTURAL CHARACTERIZATION OF ANTIBODIES MOR 6032, MOR-7361, MOR-7480, and MOR-7483 60 The procedures described above in Examples I - 3 were used to produce several fully human anti-4-1BSB igG2 antibodies, including antibodies designated as "MOR 6032", "MOR-7361", "MOR-7480", and "MOR-7483." The cDNA sequences encoding the heavy and light chain variable regions of the MOR-6032, MOR-7361, MOR-7480, 5 and MOR-7483 monoclonal antibodies were obtained using standard PCR techniques and were sequenced using standard DNA sequencing techniques. The nucleotide and amino acid sequences of the heavy chain variable region, full length heavy chain of the igG2 subclass, light chain variable region, and full length light chain of antibodies MOR-6032, MOR-7361, MOR-7480, MOR-7480.1, MOR-7480.2, 10 MOR-7483, MOR-7483.1, and MOR-7483.2 are provided in this disclosure; an index of the SEQ ID NOs for these sequences is provided in Table 1. Comparison of the MOR-6032 heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the MOR-6032 heavy chain utilizes a VH segment from human germline VH 1-69, a D 15 segment from the human germline 4-23, and a JH segment from human germline JH 4a. Further analysis of the MOR-6032 VH sequence using the Kabat system of CDR region determination led to the delineation of the H-CDR1, H-CDR2 and H-CDR3 regions as shown in SEQ ID NOs: 1, 2 and 3, respectively. 20 Comparison of the MOR-7361 heavy chain immunoglobulin sequence to the known human germline immunoglobulin heavy chain sequences demonstrated that the 7361 heavy chain utilizes a VH segment from human germline VH 3-23, a D segment from the human germline 2-8, and a JH segment from human germline JH 4a. Further analysis of the MOR-7361 VH sequence using the Kabat system of CDR 25 region determination led to the delineation of the heavy chain H-CDR1, H-CDR2 and H CDR3 regions as shown in SEQ ID NOs: 15, 16 and 17, respectively. Comparison of the MOR-7480 and MOR-7483 heavy chain immunoglobulin sequences to the known human germline immunoglobulin heavy chain sequences demonstrated that the 7480 and 7483 heavy chains utilize a VH segment from human 30 germline VH 5, a D segment from the human germline 5-18, and a JH segment from human germline JH 4a. Further analysis of the 7480 and 7483 VH sequences using the Kabat system of CDR region determination led to the delineation of the H-CDR1, H-CDR2 and H-CDR3 regions as shown in SEQ ID NOs: 29, 30 and 31, respectively.

61 Comparison of the MOR-6032, MOR-7361, MOR-7480 and MOR-7483 light chain immunoglobulin sequences to the known human germline immunoglobulin light chain sequences demonstrated that the 6032, 7361, 7480 and 7483 light chains all utilize a VL segment from human germline A3-r and a JL segment from human germline 5 JL 3b. Further analysis of the MOR-6032 VL sequence using the Kabat system of CDR region determination led to the delineation of the light chain CDR1, CDR2 and CDR3 regions as shown in SEQ ID NOs: 6, 7, and 8, respectively. Further analysis of the MOR-7361 VL sequence using the Kabat system of CDR 10 region determination led to the delineation of the L-CDR1, L-CDR2 and L-CDR3 regions as shown in SEQ ID NOs: 20, 21, and 22, respectively. Further analysis of the MOR-7480 VL sequence using the Kabat system of CDR region determination led to the delineation of the L-CDR1, L-CDR2 and L-CDR3 regions as shown in SEQ ID NOs: 34, 35, and 36, respectively. 15 Further analysis of the MOR-7483 VL sequence using the Kabat system of CDR region determination led to the delineation of the L-CDR1, L-CDR2 and L-CDR3 regions as shown in SEQ ID NOs: 34, 35, and 55, respectively. EXAMPLE: GERMLINED VERSIONS OF ANTIBODIES MOR-7480 and 20 MOR-7483 In order to minimize immunogenicity of the MOR-7480 and MOR-7483 antibodies, several amino acid residues were mutated back to germline sequence, as follows. A germlined version of MOR-7480, designated MOR-7480.1, was prepared by returning two amino acids in the FRI region of the heavy variable chain to germline 25 sequence. More specifically, Q at amino acid residue number 1 was returned to the germline E, and K at amino acid residue number 19 was returned to R. The two amino acid residues that were changed in the heavy chain variable region can be seen by comparing the amino acid sequence of MOR-7480 (SEQ ID NO:32) with MOR-7480.1 (SEQ ID NO:43). In the light chain variable region of MOR-7480, five amino acids in the 30 FRI region (D1S, 12Y, A13S,R19S, S21T), two amino acids in the FR2 region (A42S, V45L), and one in the FR3 region (E80M) were reverted to germline sequence. The eight amino acids that were changed in the light chain variable region can be seen by comparing the amino acid sequence of MOR-7480 (SEQ ID NO:37) with that of MOR 7480.1 (SEQ ID NO:45).

62 Moreover, a third version of MOR-7480 was prepared by starting with the light chain variable region sequence of MOR-7480.1 (SEQ ID NO:45) and reverting L45 back to V to produce MOR-7480.2 (SEQ ID NO:51). A "germlined" version of MOR-7483, designated MOR-7483.1, was prepared by 5 backmutating two amino acids in the FR1 region of the heavy variable chain to germline sequence. The germlined versions can be prepared by starting with the germline version of the antibody chain and then changing the desired amino acids in the CDRs, or any combination of mutations starting from any version. To produce MOR-7483.1, Q at amino acid residue number I was returned to the germline E, and K at amino acid 10 residue number 19 was returned to R. The two amino acid residues that were changed in the heavy chain variable region can be seen by comparing the sequence of MOR 7483 (SEQ ID NO:32) with MOR-7483.1 (SEQ ID NO:43). In the light chain variable region of MOR-7483, five amino acids in the FR1 region (DIS, 12Y, A13S, R19S, S21T), two amino acids in the FR2 region (A42S, V45L), and one in the FR3 region (E80M) 15 were reverted to germline sequence. The eight amino acids that were changed in the light chain variable region can be seen by comparing the amino acid sequence of MOR 7483 (SEQ ID NO:56) with that of MOR-7483.1 (SEQ ID NO:60). Moreover, a third version of MOR-7483 was prepared by back mutating L45 of the light chain variable region sequence of MOR-7483.1 (SEQ ID NO:60) to the 20 germline V45 to produce MOR-7483.2 (SEQ ID NO:64). EXAMPLE 6: IN VITRO PROPERTIES OF ANTIBODIES, INCLUDING GERMLINED VERSIONS Binding Affinities of Antibodies (BlAcore Assay) Binding kinetics of certain antibodies binding human 4-1BB were measured by 25 surface plasmon resonance (SPR) technology using a Biacore 3000 instrument (GE Healthcare). Recombinant human 4-1BB/Fc Chimera protein comprising amino acids 24 - 186 of SEQ ID NO: 68 was purchased from R&D Systems Inc. (#838-4B). The lyophilized protein was dissolved in a buffer containing 150 mM NaCl, 25mM HEPES pH 8.0, 6 mM MgCI 2 , 0.005% polysorbate 20, and 0.5 mM sodium azide to a final 30 concentration of 80 nM based on the predicted molecular weight (44.8 kDa) provided by the R&D Systems. The Fc portion of the molecule was cleaved by treatment with Bovine Factor Xa (Pierce, #32521) in 150 mM NaCl, 25mM HEPES pH 8.0, 6 mM MgClz 0.005% polysorbate 20, 0.5mM sodium azide, using a 20 hour incubation at 22*C with a 3% Factor Xa (3 pg Factor Xa per 100 pg 4-1BB chimera). The 4-1BB portion of the 63 molecule comprises amino acid residues 24 though 186 of the human 4-1BB protein. Binding experiments were carried out at 25*C in a running buffer comprising 150 mM NaCl, 25mM HEPES pH 8.0, 6 mM MgC 2 , 0.005% polysorbate 20, and 0.5 mM sodium azide. Antibodies were immobilized by standard amine coupling to a CM5 sensorchip 5 (GE Healthcare) using a 0.1 mg/mL solution of the antibody in 10 mM sodium acetate at pH 5.0. The 4-1BB was injected at a range of concentrations from 80 nM to 0.16 nM, at a 50 pL/minute flow rate, for 3.6 minutes followed by a 26 minute dissociation period using the Kinject feature of the Biacore 3000 instrument. The bound complex was regenerated by a 1 minute pulse of 10 mM phosphoric acid in water. Data analysis was 10 performed using the Scrubber2 software (BioLogic Software). Sensograms were fit to a simple 1:1 Langmuir binding model. The antibodies were shown to reversibly bind to recombinant human 4-1BB. The results (average values) are presented in Table 7. Binding to the extracellular domain of 4-1 BB (ELISA assay) Human 4-1BB IgG1Fc chimera (R&D Systems, Minneapolis, MN) was 15 resuspended with Dulbecco's Phosphate Buffered Saline (DPBS) containing 0.1% bovine serum albumin (BSA) to 0.2mg/ml and diluted with DPBS to a final concentration of 0.03 ug/mI. Nunc-Immuno Maxisorp 96 well plates were coated with O.1ml per well of the recombinant 4-1BB chimera leaving empty wells for nonspecific binding controls and incubated at 4"C overnight. The 4-1BB solution was removed and the plates were 20 washed three times with 0.2ml wash buffer (0.05% Tween-20 in DPBS). 0.2ml blocking buffer (5% BSA, 0.05% Tween-20 in DPBS) was added to all wells and incubated at 4*C for 1 hour with mixing. The blocking buffer was removed and plates washed three times with 0.2ml wash buffer. Serial dilutions of the 4-IBB test antibodies were prepared in DPBS and O.1ml diluted Ab was added per well. Plates were incubated 1.5 hour at 25 room temperature. Antibody solution was removed and the plates washed three times with 0.2ml wash buffer per well. Horseradish peroxidase labeled goat anti-human IgG, F(ab')2 specific F(ab')2 antibody (Jackson immunoresearch #109-036-097, West Grove, PA) was diluted 1:5000 with DPBS and added O.1ml per well. The plates were incubated 1 hour at room temperature and washed with 0.2ml per well wash buffer. 30 O.1ml SureBlue TMB microwell peroxidase substrate (Kirkegaard & Perry Labs, Gaithersburg, MD) was added and incubated for 20 minutes at room temperature. The reaction was stopped by adding an equal volume of 2M H 2

SO

4 and absorbance was read at 450 nm on a Molecular Devices Spectra Max 340 (Molecular Devices, Sunnyvale, CA). The results are presented in Table 8.

64 Ligand Competition Binding (ELISA Assay) Antibodies were tested for their ability to block the binding of the human 4 1 BB IgGl Fc chimera to plate bound recombinant 4-1 BB ligand (4-1 BBL). Recombinant human 4-1BB ligand (Biosource/invitrogen, Carlsbad, CA) was resuspended to 0.2 5 mg/mL in DPBS + 0.1% bovine serum albumin and then diluted to 1 pg/mL in DPBS. Nunc-Immuno MaxiSorp surface 96 well plates were coated with 0.1 mLlwell of the 4 1 BBL solution overnight at 4 C. The following day the 4-1 BBL solution was removed and 0.2 mL Blocking buffer (1% bovine serum albumin, 0.05% Tween-20 in DPBS) added and incubated at room temperature for 2 hours. During the blocking step the 10 antibody stocks were diluted in a range from 8 ng/mL to 6 pg/mL in DPBS. Recombinant human 4-IBB-IgG1Fc (R&D Systems, Minneapolis, MN) was resuspended to 0.2 mg/mL in DPBS + 0.1% bovine serum albumin and then diluted to 0.02 pg/mL in DPBS. The blocked 4-IBBL coated plates were washed three times with 0.2 mL wash buffer (0.05% Tween 20 in DPBS). 60 pL antibody dilutions were added 15 along with 60 pL 4-1BBIgG1Fc chimera and incubated at room temperature for 1.5 hours. Plates were washed as described previously. Horseradish peroxidase anti 6xHistidine tag antibody (R&D Systems, Minneapolis MN #MABO50H) was diluted 1:1000 in DPBS, 50 pL of the resulting solution added to the wells of the washed plates, and incubated at room temperature for 1 hour. Plates were washed as previously 20 described, 50 pL TMB substrate solution was added to each well and incubated at room temperature for 20 minutes. The reaction was stopped with 50 pL 0.2N H 2

SO

4 and absorbance at 450 nm read using a Molecular Devices plate reader. The results are presented in Table 8. 25 Species Cross-Reactivity of Antibodies The species-cross reactivity of the exemplary antibodies was measured using phytohemagglutinin (PHA) stimulated primary peripheral blood mononuclear cells (PBMC) of human, cynomolgus monkey (cyno), dog, and rat. Cells were isolated according to the procedure described below. Cells (-5OxOcells/tube) were washed 30 once in cold wash buffer (PBS, 2% FBS and 0.02% sodium azide) and 100 pl/tube of Alexa Fluor 647 conjugated control or 4-1 BB reactive antibodies at 15.5 pg/mL (100 nM) was added to each sample along with labeled species specific T cell marker antibodies. The T cell marker antibodies utilized are as follows, FITC anti-human CD3e (BD Pharmingen, #555332), FITC anti-rat CD3e (BD Pharmingen, #559975), FITC anti- 65 rabbit CD4 + FITC anti-rabbit CD8 (AbD Serotec, #MCA799F and #MCA1576F), FITC anti-dog CD3e (AbD Serotec, #MCA1774F), and PerCP anti-human/cyno CD3e (BD Pharmingen, #552851). The cells were incubated in the dark with fluorochrome antibodies on ice for 30 minutes, washed three times and resuspended in 0.3 ml wash 5 buffer for analysis. Antibody staining was measured and analyzed using a Becton Dickinson FACS Calibur and FlowJo 8.8.2 software. Isolation of Human T Lymphocytes. Human whole blood was collected into syringes containing 1 mL 0.5M EDTA and then transferred to Sigma Accuspin tubes (Sigma, St. Louis, MO) for the isolation of peripheral blood mononuclear cells (PBMC) 10 as described by the manufacturer. The PBMCs were washed twice with DPBS containing 5 mM EDTA and T lymphocytes were isolated using a T cell purification column as described by the manufacturer (R&D Systems, Minneapolis, MN). Briefly, PBMCs were resuspended in 2 mL of column buffer and loaded into a pre-washed T cell isolation column. PBMCs were incubated for 10 minutes at room temperature and T 15 cells were eluted with column buffer, washed one time and resuspended TCM at 2xlO 6 cells/mL consisting of RPMI 1640 (Sigma, St Louis, MO) supplemented with 10% fetal bovine serum (Sigma, St. Louis, MO) and L-glutamine (2 mM), Hepes (10 mM), penicillin (100 U/ml), streptomycin (50 ug/ml) (Gibco, Grand Island, NY.). Isolation of Cynomolaus PBMCs. Cynomolgus whole blood (Bioreclamation; 20 Hicksville, NY) was collected in sodium citrate CPT vacutainer tubes (BD; Franklin Lakes, NJ) and then spun at 1500 x g for 20 minutes at room temperature. Tubes were shipped overnight at 4 0 C. The PBMC fraction was collected from the CPT tubes and washed 2x with PBS containing 5mM EDTA. Following the wash step, PBMCs were counted and readjusted to 2x10 6 cells/mL in tissue culture medium (TCM). TCM 25 consisted of RPMI 1640 (Sigma, St Louis, MO) supplemented with 10% fetal bovine serum (Sigma, St Louis, MO) and L-glutamine (2 mM), HEPES (10 mM), penicillin (100 U/mL), streptomycin (50 gg/mL) purchased from Gibco (Grand Island, NY.). Cells were stimulated with 10 pg/mL PHA 2-3 days to induce expression of 4-1 BB, Isolation of Canine PBMCs. Canine whole blood was drawn into heparinized 30 vacutainer tubes (BD; Franklin Lakes, NJ) and diluted 1:2 with PBS containing 5mM EDTA. After mixing, 4 mL of the diluted blood was carefully layered over 3mL Lympholyte-Mammal (Cedarlane Laboratories, Westbury, NY) and centrifuged 800 x g for 20 minutes at 25*C. The PBMC interface was collected, washed twice with PBS and resuspended to 2xl0' cells/mL in TCM containing PHA at 10 pg/mL (Remel, Lenexa, 66 KS). The cells were cultured for 48-72 hours prior to testing for antibody binding by flow cytometry. Isolation of Rat PBMCs. Rat whole blood was drawn into heparinized vacutainer tubes (BD; Franklin Lakes, NJ) and diluted 1:3 with PBS containing 5 mM 5 EDTA. After mixing, 6 mL of the diluted blood was carefully layered over 4.5ml Lympholyte-Mammal (Cedarlane Laboratories, Westbury, NY) and centrifuged 800 x g for 20 minutes at 25 0 C. The PBMC interface was collected, washed twice with PBS and resuspended to 2x1O"cells/mL in TCM containing PHA at 10 pg/mL (Remel, Lenexa, KS). The cells were cultured for 48-72 hours prior to testing for antibody binding by flow 10 cytometry. The binding results are provided in Figure 1. The antibodies were found to bind to the human and cyno 4-1 BB with high affinity, while binding to dog and rat 4-1 BB was not observed at concentrations of 100 nM, the highest concentration tested. Table 7: Binding Affinities of IgG Antibodies (Biacore) Antibody k. (M- s-) k (s') Ko (nM) tv2 MOR7480 (IgGl) 3.6 x1O 1.5 x1074 0.42 82 min MOR7480 (IgG2) 4.5 x1 O 2.3 x10 0.57 50 min MOR-7480.1 1.2 ±0.3 x10 9.8 ± 8.4 ± 1.4 1.2 min (IgG1) 0.15x10 3 MOR-7480.1 1.4 0.06 1.2 ± 0.1 x10- 8.7 ± 1.0 1.0 min (IgG2) x10 6 2 MOR-7480.2 9.3 x10 41 x1 0.4 28 min (IgG1) MOR-7483 (IgG1) 6.0 x10 5 4.x10 4 0.73 26 min MOR-7483 (IgG2) 30 x1O0 3.8 x10 4 1.3 1.3 min MOR-7483.1 8.0 x10 5 0.022 28 32 min (IgG1) 15 Table 8: ELISA Binding and Ligand Competition Values Binding ELISA Ligand Competition ELISA Antibody isotype EC50±STD % max iC50±STD (nM) inhibition (nM) MOR-6032 IgG1 0.071± 0.029 100±2 0.153±0.067 67 MOR-6032 IgG4 0.226± 0.161 100±2 0.112±0.023 MOR-7361 IgG1 0.091± 0.010 100±2 0,172±0,006 MOR-7480 IgG1 0.076± 0.008 96±2 0.122±0.019 MOR-7480 IgG2 0,122± 0.009 98±2 0.125±0.003 MOR-7483 IgG1 0.073± 0.024 98±2 0.109±0.028 MOR-7483 IgG2 0.165± 0.035 97±2 0.138±0.015 Epitope Mappina In order to determine the epitope binding region of the 4-1BB agonist antibodies, a series of mutations (Table 9) were made to the human 4-1 BB extracellular domain to 5 the published dog 4-1BB sequence (Reft Seq. XM_845243). Table 9. Mutant of Human 4-1BB Extracellular Domain Mutant of human 4-1BB extracellular Amino Acid Changes domain Hu41BB N&E N30K, A56T, G57S, R60K, T61A Hu41BB N&E.1 N30K, D38G, N39K, R41K, S461, A56T, G57S, R60K, T61A Hu41BB N&E.2 N30K, A56T, G57S, R60K, T61A, K69E, R75K, E77V Hu41BB L241, P27S, N42S, T891, P90S, S1OOT N&E.3 Hu41BB L241, P27S, N30K, D38G, N39K, R41K, N42S, S461, A56T, N&E.4 G578, R60K, T61A, K69E, R75K, E77V, T891, P90S, S100T Hu41BB K115Q, C121R, R134Q, R154S, V156A N&E.5 Hu41BB N&E.6 S161A, P162S, D164G, L165F, A169T All human-to-dog mutations were prepared by Gene Dynamics LLC, (Portland, OR) in the retroviral expression vector pMSCVpuro (Clontech Laboratories Mountain 68 View, CA). Additionally the full canine cDNA sequence was prepared via gene synthesis corresponding to Ref. Seq. XM_845243. Viral preparations were established by transient transfection of roughly 40-50% confluent 293T cells in T-75 flasks. Following culture, the viral supernatant was then 5 sterile filtered, and subjected to concentration. The concentrated virus was collected and stored at -80"C. Logarithmically growing 300-19 cells were transduced with retrovirus using 1:250 dilution concentrated virus plus 8ug/ml polybrene in complete DMEM. Following a 24 hour incubation, 2ug/ml puromycin was added to the cultures and maintained during the 10 course of the study. Positive expression of the 4-1BB receptors by the puromycin selected pools was confirmed by staining with lug/ml polyclonal goat anti-human 4-1BB antibody (R&D Systems Inc.) plus 1:200 dilution PE labeled donkey anti-goat IgG (H+L) F(ab') 2 (Jackson Immunoresearch Inc.). In order to determine recognition of the mutant 4-1BB 15 receptors by the test antibodies the puromycin selected poois were stained with 1OOnM dilution of the unlabeled primary antibody on ice for 30 min, followed by two washes with FACS buffer, and 1:200 dilution species specific PE labeled donkey anti-IgG (H+L) F(ab') 2 . Cells were analyzed by FACS using a BD FACS Calibur and FlowJo 8.8.6 software. 20 Relative staining of each cell pool is summarized in Table 10. Table 10, Relative Staining of Each Cell Pool Ab W W LU LU L W o M 05 z .6 o . 6 o6 c.$ 06 Goat pAb + + + + + + + + + BBK-2 + + - + + - + + JG1.6A + + - + + - + + 4B4-1 + + - + + - + + 6032G1 + + + - + - + + - 69 7361G1 + + + - +/- - + + 7480G1 + + + + + + + + + 7480.1G1 + + + + + + + 7480.1 G2 + + + + + + +/- + 7480.2G1 + + + + + + + + + 7483_G1 + + + + + + +/- + +/ 7483G2 + + + + + + - + Differentiation of binding between antibodies having similar sequences (MOR 7480, MOR-7480.1, MOR-7480.2, MOR-7483, and MOR-74831) was discovered within the mutations of clone N&E.5, suggesting that the determinants for antibody recognition 5 lie within the mutated region. In order to determine the relative affinity of these antibodies for human 4-1BB extracellular domain and the mutant of human 4-1BB extracellular domain, mutant N&E.5, a dose response FAGS curve was determined for each antibody. Alexa Fluor 647 labeled MOR_7480, MOR 7480.1, and MOR_7480.2 were diluted in FACS buffer 10 from iuM in an 8 point 1:5 dilution series and used to stain parental 300-19, hu4-1BB, hu4-1 BB N&E.5, and dog 4-1 BB cell pools. The cells were analyzed by FACS using a BD FAGS Calibur and FlowJo 8.8.6 software. The geometric mean fluorescence of each receptor expressing pool was normalized to staining of parental cells and expressed as fold staining and an EC50 for dose response was determined. The EC50 15 summary is shown in Table 11. Greater than 5 fold decrease in binding for both MOR_7480.2 and MOR_7480 for the human 4-11BB mutant N&E.5 was noted. Table 11. Binding ECo (nM) of Antibodies 4-1BB Mutant Antibody (IgG1) Human 4-1BB N&E,5 MOR 7480.1 7.916 n/a MOR_7480.2 0.510 2.730 MOR_7480 1.68 12.29 70 Agonist Activity of Antibodies (Luciferase Activity Assay) 293T cells expressing human 4-IBB along with a stably integrated NFkB luciferase reporter were prepared. Cells were harvested, washed and resuspended into 5 phenol red free complete medium (DMEM containing 10% fetal bovine serum, HEPES buffer, nonessential amino acids and L-glutamine) at density of 0.6 X 10 6 cells/mL. 50gi of cells were plated into each assay well of a white 96 well plate (PerkinElmer, Waltham, MA). Test antibodies were added to each well in the presence 2.5:1 ratio of a cross linking antibody Fab' goat anti-human igG Fc (Jackson ImmunoResearch, West Grove, 10 PA). The plate was incubated 5 hours at 37*C. 75 gI of Bright-Glo Luciferase reagent (Promega, Madison WI) was added and the amount of luciferase activity was measured using a Packard TopCount NXT scintillation counter. 293T cells expressing cynomologous monkey 4-1BB were prepared by viral transduction and selection of a stable pool with 2pg/ml puromycin. Cyno 4-1BB 15 expressing 293T cells were plated in a T-75 flask to roughly 60-70% confluency then transfected with 10pg pLuc 6xNFkB plus 0.1pg pRL-CMV as a transfection control. Transfections were performed using Fugene 6 transfection reagent (Roche Indianapolis, IN) at a 6pl Fugene to 1 pg plasmid DNA ratio according to manufacturer instructions. Cells were harvested the following day washed and resuspended into phenol red free 20 complete medium (DMEM containing 10% fetal bovine serum, nonessential amino acids and L-glutamine) at density of 0.6 X 10 6 cells/mL. 50pl of cells were plated into each assay well of a white 96 well plate (PerkinElmer, Waltham, MA). Test antibodies were added to each well in the presence 2.5:1 ratio of a cross linking Fab' goat anti-human IgG Fc antibody (Jackson ImmunoResearch, West Grove, PA). The plate was incubated 25 5 hours at 370C. 75pl of luciferase assay reagent was added and the amount of firefly luciferase activity was measured using a Packard TopCount NXT scintillation counter. Additionally, 75pl of Stop & Gio reagent was added to assess the renilla luciferase activity. The amount of renilla luciferase activity was measured using a Pakcard TopCount NXT scintillation counter. Results are presented in Figure 2. 30 Agonist Activity of Antibodies (primary T cell IL-2 release assay) Nune Maxisorp 96 well plates were UV sterilized prior to plate coating. Test antibodies were diluted in PBS to 60 pg/ml. 0.2ml of the diluted Ab was divided to 2 wells of a polypropylene 96 well plate and serially diluted 1:3. 50pi of the diluted Ab were added to the sterile Maxisorp 96 well assay plate and immediately 50pl of 20pg/ml anti-human 71 CD3s clone UCHTI was added (Biolegend San Diego, CA). All plates were then incubated overnight at 40C. The following day, Ab coated plates were washed 1x with PBS and 0.1 5ml RPMI complete media was added to the wells of the Nune Maxisorp plates. Human T cells were isolated as described previously elsewhere herein and 50pi 5 purified T cells at 2 x 106 cells/ml (100,000 cells/well) were added to each well. Cells were incubated at 37'C, 5%CO2 for 3 days. Supernatant from each well was collected and either assayed immediately or stored at -20CC prior to assay. Supernatants were diluted with complete media prior to IL-2 ELISA assay (R&D Systems, Minneapolis, MN). Results are presented in Figure 3. 10 EXAMPLE 7: HUMAN LEUKOCYTE EXPANSION INDUCED BY 4- 1BB ANTIBODIES IN VIVO The lack of detectable cross-reactivity of the 4-1 BB antibodies with the murine 4 1BB and the requirement for the presence of human immune cells required the 15 development of models for the in vivo functional assessment of the 4-1BB antibodies. Mice with the NOD genetic background carrying the severe combined immunodeficient (SCID) mutation and deficiency in the IL-2 receptor common gamma chain (commonly termed NSG) are able to support the engraftment of large number of human peripheral blood leukocytes (huPBL) and maintain engraftment for at least 30 days (King, 2008, 20 Clin. Immunol. 126:303-314). This mouse model, also known as huPBL-NSG model, was used to assess the functional effect of in vivo systemic administration of the antibodies on human immune cells. Specifically, 6 million freshly isolated human PBMCs were adoptively transferred via intraveinous injection into NOD.Cg-Prkdc"cd Il 2 rgmri/SzJ (NSG) host mice. Nine days post PBMC injections, the animals were 25 administered a single 1mg/kg dose of MOR7480, MOR7480.1 or IgG2 isotype control antibody via intraperitoneal injection. At day 24 to 28 post PBMC engraftment, PBMC were stained with antibodies to human and murine CD45 assessed via flow cytometry. Forward and side scatter profiles were used to determine a lymphocyte gate. As shown in Figure 4, MOR7480 and MOR7480.1 were able to enhance expansion of human 30 leukocytes as evidenced by increased proportion of human CD45+ cells in the peripheral blood of engrafted mice. For each group, na6 mice. In addition, MOR7480.1 treatment of cynomolgus monkeys increased proliferation among cytotoxic central memory T cells (CD8 TcM) In PBMC samples. Cynomolgus monkeys (2 animals per dose level) were given a single intravenous 72 injection of MOR7480.1 at the indicated dose. PBMC were harvested 7 days prior to the antibody dose (pre dose) and on the indicated study days relative to administration of MOR7480.1 (on Study Day 1). PBMC were stained with antibodies for CD3, CD4, CD8, CD28, CD95, and Ki-67 and analyzed via flow cytometry. Data was collected on a 5 Canto II (Beckton Dickinson) and analyzed using DIVA software (Becton Dickinson). CD8 central memory cells were identified as CD3+, CD8+, CD28+ and CD95+. Data is shown for individual animals designated as (dose level-animal number) and is represented as intra-animal change in the number of Ki-67+ cells relative to pre study number {[(#Ki-67+ cells on indicated study day - #Ki-67+ cells at pre dose)/#Ki-67+ 10 cells at pre dose*100}. As shown in figure 5, A 1.5 fold or greater increase in proliferating central memory T cells during the first 7-13 days of study was noted in at least one animal of all groups treated with 0.3 mg/kg or greater. EXAMPLE 8: ANTI-TUMOR ACTIVITY OF 4-1BB ANTIBODIES (IN ViVO 15 MODEL) PC3 Human Prostate Cancer Model. The lack of rodent cross reactivity of the 4-1 BB antibodies prevented the use of standard murine syngeneic or human xenograft tumor models for the assessment of anti-human tumor efficacy of the antibodies. Accordingly, a novel huPBL-SCID-Bg 20 xenogenic tumor mouse model was generated using a SCID-Bg mouse (CB.17/lcr.Cg Pkrdcst"dLyStb/Cr), which harbors the beige (Bg) mutation lack murine T and B lymphocytes and functional NK cells. The anti-human tumor efficacy of the 4-1BB antibodies was assessed using this model as described below. The PC3 human prostate or LOVO human colon cell line was obtained from 25 American Type Culture Collection and was cultured in RPMI-1640 (Invitrogen) enriched with the following Invitrogen supplements: L-Glutamine, Sodium pyruvate, non-essential amino acids, penicillin/streptomycin, Hepes, and 10% heat inactivated fetal bovine serum (FBS; Cat. No. F2442, Sigma Aldrich). Cells were grown to confluency in T-225 Falcon flasks. Subsequently, cells were trypsinized (Trypsin 0.25%-EDTA; Cat. No. 30 2500-056, Invitrogen) and growth was scaled up in Hyperflasks (Cat. No. 3319 Corning Life Sciences) for three days. Trypsin was used to harvest the cell line which was washed 3 times in ice cold PRMI supplemented with 10% FBS. No greater than 300 ml of peripheral blood was collected from healthy volunteers. Peripheral blood lymphocytes (PBMCs) were isolated from heparinized blood using Accuspin tubes in 73 accordance with the manufactures' protocol (Cat. No. A0561-100x 15 ml, Aldrich). Counted cell suspensions were combined such that each mouse received an injection of 1.5x 106 PBMCs and 3 x 106 tumor cells in a single bolus injection of 0.2 mL in PBS. The combined cell suspension was washed twice with cold PBS, placed on ice and 5 immediately injected into prepared mice. For each mouse, a 0.2 mL volume of the combined cell suspension was injected subcutaneously into the right flank of the animal and given a single dose (0.2 mL) of the 4-1BB antibody or control antibody by subcutaneous injection into the left flank. Tumor measurements were made via Pressier caliper twice per week for the duration of the 10 experiments and body weights were also recorded. Tumor volume was calculated using the following calculation: length x width 2 x 0.44= volume (mm 3 ) Mice were removed from the study in the event that the tumor volume reached 2000 mm 3 or an animal lost 20% of body weight before termination of the experiment. On day 23 mice from all groups were euthanized in accordance with procedures outlined by the IACUC (Figure 6). 15 Percent tumor growth inhibition was measured on the final day of the study and is calculated as 100-{1-(Treated final day/Control tin day)). Similar results were observed when tumors were measured on day 6 post injection, and the animals were randomized according to tumor volume, and given a single 4-1BB mAb dose on day 7 post implantation. For most studies, each group contained 8 mice. 20 EXAMPLE 9: IN VIVO ASSESSMENT OF ACTIVITY OF 4-1BB ANTIBODIES IN HUMAN 4-1BB KNOCK IN MICE Generation of human 4-1 BB Knock-in mice To better address the immune modulating activities of anti-human 4-1BB 25 monoclonal antibodies that do not cross react with murine 4-1BB, a mouse model in which the mouse 4-1BB gene was replaced by the human 4-1BB gene was generated. Bacterial artificial chromosome (BAC) clones carrying the human or murine 4-1BB genomic fragment were ordered from Invitrogen (Carlsbad, CA.) and used to construct the 4-1BB targeting vector based on the Red/ET recombination technology (Zhang, 30 1998, Nat Genet 20:123-128). First, a retrieval vector was assembled on the pBR322 backbone such that, when opened by Xbal digestion, the two murine/human chimeric homology arms (400 bps each) will retrieve from the human 4-1BB BAC clone the 19,994 bps of human 4-1BB genomic sequence beginning with the translation start codon ATG located in exon 2 and ending with the stop codon TGA in exon 8. Second, a 74 neomycin expression cassette under the control of the PGK/EM7 promoters was assembled and flanked by 100 base pairs (bps) of sequences homologous to intron 2 sequences of the human 4-1BB gene. This neomycin expression cassette was then targeted into the retrieved human 4-1BB genomic fragment obtained in step 1. Lastly, 5 the retrieved human 4-1BB genomic fragment carrying the neomycin expression cassette was targeted into a murine BAC clone to replace the murine 4-1 BB gene with the modified human 4-1 BB genomic fragment from ATG start codon to TGA stop codon. This BAC targeting vector was electroporated into a mouse embryonic stem cell line on the C57BL/6NTAC background (PRX-BL6N #1, Primogenix, Laurie, MO.) 10 following a standard protocol and clones surviving G418 (also known as geneticin) selection were screened by two Taqman assays against intron 2 and exon 8 of the murine 4-1BB gene to identify clones that had been modified at the murine 4-1BB locus by a homologous recombination mechanism Of the 116 ES clones screened, 7 clones were found to have lost one allele of the murine 4-1BB locus (targeting efficiency 6%). 15 Karyotype analysis and in situ hybridization (FISH) were performed by Coriell Institute for Medical Research (Camden, N.J.). For clone LH15, 19 out of 20 cells were 40 XY, and for LH80, 20 out of 20 cells 40XY. In both clones, FISH using a murine BAC clone carrying the 4-1 BB gene as a probe showed one signal of 4-1BB hybridization on each of the chromosome 4 pair in the region of band E2. No signal was seen at any other 20 locations. Both clones LH15 and LH80 were injected into blastocysts of the BALB/c strain and embryos implanted into the CD1 pseudopregnant female mice to carry to term. Male chimeras were mated to the Ella-cre mice on C57BL16 background to remove the neomycin resistance cassette and mice homozygous for the human 4-1 BB gene were 25 used in study. 4-1BB Agonist mAb mediated lvmphocyte proliferation. The ability of 4-1BB agonist mAbs to induce lymphocyte proliferation was assessed in 4-1BB Knock-in mice. 4-1BB Knock-in mice were dosed via intraperitoneal 30 injection with 30 mg/kg of MOR7480.1 on study Day 0 (for weekly dosing animals received 4-1 BB mAb injections on Day 0 and Day7). 24 hours prior to sample collection, animals were injected intraperitoneally with 2 mg BrdU. At the indicated day post dose, peripheral blood samples were collected via intracardiac puncture. PBMC were stained 75 with antibodies against CD3, CD4, CD8, and BrdU and assessed via flow cytometry. Results are presented in Figure 7 panel A. 4-1BB Agonist mAb mediated anti-tumor efficacy 5 Anti-tumor efficacy of MOR7480.1 was assessed in 4-1 BB knock in mice using B16 -OVA/luc, a melanoma line that has been engineered to express the ovalbumin (OVA) model antigen and luciferase (luc). One million tumor cells were implanted on the flank of 4-1BB knock in mice. Animals were randomized based on tumor size on when tumors reached approximately 50-100 mm' (generally 7-10 days post tumor inoculation) 10 and given a single injection of the indicated dose of 4-1BB mAb. Tumor size was assessed using caliper measurement two to three times per week until study termination. Results are presented in Figure 7 panel B.

76 SEQUENCE LISTING - SEQ ID No 1: H-CDR1 of MOR-6032 antibody NSYAIS 5 - SEQ ID No 2: H-CDR2 of MOR-6032 antibody GIlPGFGTANYAQKFQG - SEQ ID No 3: H-CDR3 of MOR-6032 antibody RKNEEDGGFDH 10 - SEQ ID No 4: Amino Acid Sequence of VH of MOR-6032 antibody QVQLVQSGAEVKKPGSSVKVSCKASGGTFNSYAISWVRQAPGQGLEWMGGIIPGFGT ANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARKNEEDGGFDHWGQGTL VTVSS 15 - SEQ ID No 5: Amino Acid Sequence of Full Length Heavy Chain (IgG2) of MOR 6032 antibody OVOLVQSGAEVKKPGSSVKVSCKASGGTFNSYAISWVRQAPGQGLEWMGGIIPGFGT ANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARKNEEDGGFDHWGQGTL 20 VTVSSastkgpsvfplapcsrstsestaalgcivkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtvpssnfg tqtytcnvdhkpsntkvdktverkccvecppcpappvagpsvflfppkpkdtmisrtpevtcvvvdvshedpevqfnwy vdgvevhnaktkpreeqfnstfrvvsvltvvhqdwlngkeykckvsnkgpapiektisktkgqprepqvytlppsreemtk nqvsltclvkgfypsdiavewesngqpennykttppmldsdgsfflyskltvdksrwqqgnvfscsvmhealhnhytqksI slspgk 25 - SEQ ID No 6: L-CDR1 of MOR-6032 antibody SGDNLGDYYAS - SEQ ID No 7: L-CDR2 of MOR-6032 antibody 30 DDSNRPS - SEQ ID No 8: L-CDR3 of MOR-6032 antibody

QTWDGTLHFV

77 - SEQ ID No 9: Amino Acid Sequence of VL of MOR-6032 antibody DIELTQPPSVSVAPGQTARISCSGDNLGDYYAS WYQQKPGQAPVLVIYDDSNRPSGIP ERFSGSNSGNTATLTISGTQAEDEADYYCQTWDGTLHFVFGGGTKLTVL 5 - SEQ ID No 10: Amino Acid Sequence of Full Length Light Chain of MOR-6032 antibody DIELTQPPSVSVAPGOTARISCSGDNLGDYYASWYQQKPGQAPVLVIYDDSNRPSGIP ERFSGSNSGNTATLTISGTQAEDEADYYCQTWDGTLHFVFGGGTKLTVLgqpkaapsvtlfp psseelqankatlvclisdfypgavtvawkadsspvkagvetttpskqsnnkyaassylsltpeqwkshrsyscqvtheg 10 stvektvaptecs - SEQ ID No 11: Nucleic Acid Sequence of VH of MOR-6032 antibody Caggtgcaattggttcagtctggcgcggaagtgaaaaaaccgggcagcagcgtgaaagtgagctgcaaagcctccgg aggcacttttaattcttatgctatttcttgggtgcgccaagcccctgggcagggtctcgagtggatgggcggtatcattccgggt 15 tttggcactgcgaattacgcgcagaagtttcagggccgggtgaccattaccgcggatgaaagcaccagcaccgcgtatat ggaactgagcagcctgcgtagcgaagatacggccgtgtattattgcgcgcgtaagaatgaggaggatggtggttttgatca ttggggccaaggcaccctggtgacggttagctca - SEQ ID No 12: Nucleic Acid Sequence of VL of MOR-6032 antibody 20 Gatatcgaactgacccagccgccttcagtgagcgttgcaccaggtcagaccgcgcgtatctcgtgtagcggcgataatctt ggtgattattatgcttcttggtaccagcagaaacccgggcaggcgccagttcttgtgatttatgatgattctaatcgtccctcag gcatcccggaacgctttagcggatccaacagcggcaacaccgcgaccctgaccattagcggcactcaggcggaagac gaagcggattattattgccagacttgggatggtactcttcattttgtgtttggcggcggcacgaagtaaccgttctt 25 - SEQ ID No 13: Nucleic Acid Sequence of Full Length Heavy Chain of MOR-6032 antibody caggtgcaattggttcagtctggcgcggaagtgaaaaaaccgggcagcagcgtgaaagtgagctgcaaagcctccgga ggcacttttaattcttatgctatttcttgggtgcgccaagcccctgggcagggtctcgagtggatgggcggtatcattccgggttt tggcactgcgaattacgcgcagaagtttcagggccgggtgaccaftaccgcggatgaaagcaccagcaccgcgtatatg 30 gaactgagcagcctgcgtagcgaagatacggccgtgtattattgcgcgcgtaagaatgaggaggatggtggttttgatcatt ggggccaaggcaccctggtgacggttagctcagcctccaccaagggcccatggtcttccccctggcgccctgctccagg agcacctccgagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaact caggcgctotgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactccctcagcagcgtagtga ccgtgccctccagcaacttcggcacccagacctacacctgcaacgtagatcacaagcccagcaacaccaaggtggac 78 aagacagttgagcgcaaatgttgtgtcgagtgcccaccgtgcccagcaccacctgtggcaggaccgtcagtttcctcttcc ccccaaaacccaaggacaccctcatgatctcccggacccctgaggtcacgtgcgtggtggtggacgtgagccacgaag accccgaggtccagttcaactggtacgtggacggcgtggaggtgcataatgccaagacaaagccacgggaggagcag ttcaacagcacgttccgtgtggtcagcgtcctcaccgtcgtgcaccaggactggctgaacggcaaggagtacaagtgcaa 5 ggttccaacaaaggcctcccagcccccatcgagaaaaccatctccaaaaccaaagggcagccccgagaaccacag gtgtacaccctgcccccatcccgggaggagatgaccaagaaccaggtcagcctgacctgcctggtcaaaggcttctacc ccagcgacatcgccgtggagtgggagagcaatgggcagccggagaacaactacaagaccacacctcccatgctgga ctccgacggctccttcttcctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaacgtcttctcatgctc cgtgatgcatgaggctctgcacaaccactacacacagaagagcctctccctgtctccgggtaaa 10 - SEQ ID No 14: Nucleic Acid Sequence of Full Length Light Chain of MOR-6032 antibody Gatatcgaactgacccagccgccttcagtgagogttgcaccaggtcagaccgcgcgtatctcgtgtagcggcgataatctt ggtgattattatgcttcttggtaccagcagaaacccgggcaggcgccagttcttgtgatttatgatgattctaatcgtccctcag 15 gcatcccggaacgctttagcggatccaacagcggcaacaccgcgaccctgaccattagcggcactcaggcggaagac gaagcggattattattgccagactgggatggtactcttcatttgtgtttggcggcggcacgaagttaaccgttcttggtcagcc caaggctgccccctcggtcactctgttcccaccctcctctgaggagcttcaagccaacaaggccacactggtgtgtctcata agtgacttctacccgggagccgtgacagtggcctggaaggcagatagcagccccgtcaaggcgggagtggagaccac cacaccctccaaacaaagcaacaacaagtacgcggccagcagctacctgagcctgacgcctgagcagtggaagtccc 20 acagaagctacagctgccaggtcacgcatgaagggagcaccgtggagaagacagtggcccctacagaatgttca - SEQ ID No 15: H-CDR1 of MOR-7361 antibody SDYYMH 25 - SEQ ID No 16: H-CDR2 of MOR-7361 antibody VISGSGSNTYYADSVKG - SEQ ID No 17: H-CDR3 of MOR-7361 antibody RLYAQFEGDF 30 - SEQ ID No 18: Amino Acid Sequence of VH of MOR-7361 antibody QVQLVESGGGLVQPGGSLRLSCAASGFTFSDYYMHWVRQAPGKGLEWVSVISGSGS NTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARLYAQFEGDFWGQGTL

VTVSS

79 - SEQ ID No 19: Amino Acid Sequence of Full Length Heavy Chain (IgG2) of MOR-7361 antibody QVQLVESGGGLVQPGGSLRLSCAASGFTFSDYYMHWVRQAPGKGLEWVSVISGSGS 5 NTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARLYAQFEGDFWGQGTL VTVSSastkgpsvfplapcsrstsestaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtvpssnfg tqtytcnvdhkpsntkvdktverkccvecppcpappvagpsvflfppkpkdtlmisrtpevtcvvvdvshedpevqfnwy vdgvevhnaktkpreeqfnstfrvsvtvvhqdwngkeykckvsnkgipapiektisktkgqprepqvytppsreemtk nqvsltclvkgfypsdiavewesngqpennykttppmldsdgsfflyskltvdksrwqqgnvfscsvmhealhnhytqksI 10 slspgk - SEQ ID No 20: L-CDR1 of MOR-7361 antibody SGDNIGSKYVS 15 - SEQ ID No 21: L-CDR2 of MOR-7361 antibody SDSERPS - SEQ ID No 22: L-CDR3 of MOR-7361 antibody 20 QSWDGSISRV - SEQ ID No 23: Amino Acid Sequence of VL of MOR-7361 antibody DIELTQPPSVSVAPGQTARISCSGDNIGSKYVSWYQQKPGOAPVLVIYSDSERPSGIPE RFSGSNSGNTATLTISGTQAEDEADYYCQSWDGSISRVFGGGTKLTVL 25 - SEQ ID No 24: Amino Acid Sequence of Full Length Light Chain of MOR-7361 antibody DIELTQPPSVSVAPGQTARISCSGDNIGSKYVSWYQQKPGQAPVLVIYSDSERPSGIPE RFSGSNSGNTATLTISGTQAEDEADYYCQSWDGSISRVFGGGTKLTVLgqpkaapsvtlfpp 30 sseelqankatlvclisdfypgavtvawkadsspvkagvetttpskqsnnkyaassylsltpeqwkshrsyscqvthegst vektvaptecs - SEQ ID No 25: Nucleic Acid Sequence of VH of MOR-7361 antibody 80 caggtgcaattggtggaaagcggcggcggcctggtgcaacogggcggcagcctgcgtctgagctgcgcggcctccgga tttaccttttctgattattatatgcattgggtgcgccaagcccctgggaagggtctcgagtgggtgagcgttatctctggttctggt agcaatacctattatgcggatagcgtgaaaggccgttttaccatttcacgtgataattcgaaaaacaccctgtatctgcaaat gaacagcctgcgtgcggaagatacggccgtgtaftattgcgcgcgtctttatgctcagtttgagggtgatttttggggccaag 5 gcaccctggtgacggttagctca - SEQ ID No 26: Nucleic Acid Sequence of V of MOR-7361 antibody gatatcgaactgacccagccgccttcagtgagcgttgcaccaggtcagaccgcgcgtatctcgtgtagcggcgataatatt ggttctaagtatgtttcttggtaccagcagaaacccgggcaggcgccagttcttgtgatttattctgattctgagcgtcctcag 10 gcatcccggaacgctttagcggatccaacagcggcaacaccgcgaccctgaccattagcggcactcaggcggaagac gaagcggattattattgccagtettgggatggttctatttctcgtgtgtttggcggcggcacgaagttaaccgtcctaggtcag - SEQ ID No 27: Nucleic Acid Sequence of Full Length Heavy Chain of MOR-7361 15 antibody caggtgcaattggtggaaagcggcggcggcctggtgcaaccgggcggcagcctgcgtctgagctgcgcggcctccgga tttacctttctgattaftatatgcattgggtgcgccaagcccctgggaagggtctcgagtgggtgagcgttatctctggttctggt agcaatacctattatgcggatagogtgaaaggccgttttaccatttcacgtgataattcgaaaaacaccctgtatctgcaaat gaacagcctgcgtgcggaagatacggccgtgtaftattgcgcgcgtctttatgctcagtttgagggtgatttttggggccaag 20 gcaccctggtgacggttagctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctcc gagagcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgctc tgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactccctcagcagcgtagtgaccgtgccctc cagcaacttcggcacccagacctacacctgcaacgtagatcacaagcccagcaacaccaaggtggacaagacagttg agcgcaaatgttgtgtcgagtgcccaccgtgcccagcaccacctgtggcaggaccgtcagtcttcctcttccccccaaaac 25 ccaaggacaccctcatgatctcccggacccctgaggtcacgtgcgtggtggtggacgtgagccacgaagacccogagg tccagttcaactggtacgtggacggcgtggaggtgcataatgccaagacaaagccacgggaggagcagttcaacagca cgttccgtgtggtcagcgtcctcaccgtcgtgcaccaggactggtgaacggcaaggagtacaagtgcaaggtctccaac aaaggcctcccagcccccatcgagaaaaccatctccaaaaccaaagggcagccccgagaaccacaggtgtacaccc tgcccccatcccgggaggagatgaccaagaaccaggtcagcctgacctgcctggtcaaaggcttctaccccagcgacat 30 cgccgtggagtgggagagcaatgggcagccggagaacaactacaagaccacacctcccatgctggactccgacggct ccttcttcctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaacgtcttctcatgctccgtgagcatg aggctctgcacaaccactacacacagaagagcctctccctgtctccgggtaaa 81 SEQ ID No 28: Nucleic Acid Sequence of Full Length Light Chain of MOR-7361 antibody gatatcgaactgacccagccgccttcagtgagcgttgcaccaggtcagaccgcgcgtatctcgtgtagcggcgataatatt ggttctaagtatgtttcttggtaccagcagaaacccgggcaggcgccagttcttgtgatttattctgattctgagcgtccctcag 5 gcatcccggaacgctttagcggatccaacagcggcaacaccgogaccctgaccattagcggcactcaggcggaagac gaagcggattattattgccagtcttgggatggttctatttctcgtgtgtttggcggcggcacgaagttaaccgtcctaggtcag - SEQ ID No 29: H-CDR1 of MOR-7480; MOR-7480.1; MOR 7480.2; MOR-7483; MOR-7483.1; MOR-7483.2 antibody 10 STYWIS - SEQ ID No 30: H-CDR2 of MOR-7480; MOR-7480.1 MOR 7480.2; MOR-7483; MOR-7483.1; MOR-7483.2 antibody KIYPGDSYTNYSPSFQG 15 - SEQ ID No 31: H-CDR3 of MOR-7480; MOR-7480.1; MOR 7480.2; MOR-7483; MOR-7483.1; MOR-7483,2 antibody RGYGIFDY 20 - SEQ ID No 32: Amino Acid Sequence of VH of MOR-7480; MOR 7483 antibody QVQLVQSGAEVKKPGESLKISCKGSGYSFSTYWISWVRQMPGKGLEWMGKIYPGDSY TNYSPSFQGQVTISADKSISTAYLOWSSLKASDTAMYYCARGYGIFDYWGQGTLVTVS S 25 - SEQ ID No 33: Amino Acid Sequence of Full Length Heavy Chain (IgG2) of MOR-7480; MOR 7483 antibody QVQLVQSGAEVKKPGESLKISCKGSGYSFSTYWISWVRQMPGKGLEWMGKYPGDSY TNYSPSFQGQVTISADKSISTAYLQWSSLKASDTAMYYCARGYGIFDYWGQGTLVTVS 30 Sastkgpsvfplapcsrstsestaalgcvkdyfpepvtvswnsgatsgvhtfpavlqssglyslssvvtvpssnfgtqtytc nvdhkpsntkvdktverkccvecppcpappvagpsvflfppkpkdtimisrtpevtcvvvdvshedpevqfnwyvdgve vhnaktkpreeqfnstfrvvsvitvvhqdwlngkeykckvsnkglpapiektisktkgqprepqvytlppsreemtknqvslt cdvkgfypsdiavewesngqpennykttppmldsdgsfflyskltvdksrwqqgnvfscsvmhealhnhytqkslslspg k 82 - SEQ ID No 34: L-CDRi of MOR-7480; MOR-7480.1; MOR 7480.2; MOR-7483; MOR-7483.1; MOR-7483.2 antibody SGDNIGDQYAH 5 - SEQ ID No 35: L-CDR2 of MOR-7480; MOR-7480.1; MOR 7480.2; MOR-7483; MOR-7483.1; MOR-7483.2 antibody QDKNRPS 10 - SEQ ID No 36: L-CDR3 of MOR-7480; MOR-7480.1; MOR 7480.2 antibody ATYTGFGSLAV - SEQ ID No 37: Amino Acid Sequence of VL of MOR-7480 antibody DIELTQPPSVSVAPGQTARISCSGDNIGDQYAHWYQQKPGQAPVVVYQDKNRPSGIP 15 ERFSGSNSGNTATLTISGTQAEDEADYYCATYTGFGSLAVFGGGTKLTVL - SEQ ID No 38: Amino Acid Sequence of Full Length Light Chain of MOR-7480 antibody 20 DIELTOPPSVSVAPGQTARISCSGDNIGDQYAHWYQQKPGQAPVVVYQDKNRPSGIP ERFSGSNSGNTATLTISGTQAEDEADYYCATYTGFGSLAVFGGGTKLTVLgqpkaapsvtlf ppsseelqankatlvclisdfypgavtvawkadsspvkagvetttpskqsnnkyaassylsitpeqwkshrsyscqvthe gstvektvaptecs 25 - SEQ ID No 39: Nucleic Acid Sequence of VH of MOR-7480; MOR-7483 antibody caggtgcaattggttcagagcggcgcggaagtgaaaaaaccgggcgaaagcctgaaaattagctgcaaaggttccgg atattccttttctacitattggatttcttgggtgcgccagatgcctgggaagggtctcgagtggatgggcaagatctatcgggt gatagctataccaattattctccgagctttcagggccaggtgactattagcgcggataaaagcattagcaccgcgtatcttca atggagcagcctgaaagcgagcgatacggccatgtattaftgtgcgcgtggttatggtatttttgattattggggccaaggca 30 ccctggtcaccgtctcctca - SEQ ID No 40: Nucleic Acid Sequence of VL of MOR-7480 antibody Gatatcgaactgacccagccgccttcagtgagcgttgcaccaggtcagaccgcgcgtatctcgtgtagcggcgataatatt ggtgatcagtatgctcattggtaccagcagaaacccgggcaggcgccagttgttgtgatttatcaggataagaatcgtcct 83 caggcatcccggaacgctttagcggatccaacagcggcaacaccgcgaccctgaccattagcggcactcaggcggaa gacgaagcggattattattgcgctactatactggttttggttctcttgctgtgtttggcggcggcacgaagttaaccgtccta - SEQ ID No 41: Nucleic Acid Sequence of Full Length Heavy Chain (IgG2) of 5 MOR-7480; MOR-7483 antibody caggtgcaattggttcagagcggcgcggaagtgaaaaaaccgggcgaaagcctgaaaattagctgcaaaggttccgg atattccttttctactattggatttcttgggtgcgccagatgcctgggaagggtctegagtggatgggcaagatctatcgggt gatagctataccaattattctccgagctttcagggccaggtgactattagcgcggataaaagcattagcaccgcgtatttca atggagcagcctgaaagcgagcgatacggccatgtattattgtgcgcgtggttatggtattttgattattggggccaaggca 10 ccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcgccctgctccaggagcacctccgaga gcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgctctgacc agcggcgtgcacaccitcccggctgtcctacagtcctcaggactctactccctcagcagcgtagtgaccgtgccctccagc aactteggcacccagacetacacctgcaacgtagatcacaagcccagcaacaccaaggtggacaagacagttgagcg caaatgttgtgtcgagtgcccaccgtgcccagcaccacctgtggcaggaccgtcagtcttcctcttccccccaaaacccaa 15 ggacaccctcatgatctcccggacccctgaggtcacgtgcgtggtggtggacgtgagccacgaagaccccgaggtoca gttcaactggtacgtggacggcgtggaggtgcataatgccaagacaaagccacgggaggagcagttcaacagcacgtt ccgtgtggtcagogtcctcaccgtogtgcaccaggactggctgaacggcaaggagtacaagtgcaaggtctccaacaaa ggcctcccagcccccatcgagaaaaccatctccaaaaccaaagggcagccccgagaaccacaggtgtacaccctgcc cccatcccgggaggagatgaccaagaaccaggtcagcctgacctgcctggtcaaaggcttctaccccagcgacatcgc 20 cgtggagtgggagagcaatgggcagccggagaacaactacaagaccacacctcccatgctggactccgacggctcctt cttcctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaacgtcttctcatgctccgtgatgcatgagg ctctgcacaaccactacacacagaagagcctctccctgtctccgggtaaa - SEQ ID No 42: Nucleic Acid Sequence of Full Length Light Chain of MOR-7480 25 antibody gatatcgaactgacccagccgccttcagtgagcgttgcaccaggtcagaccgcgcgtatctcgtgtagcggcgataatatt ggtgatcagtatgotcattggtaccagcagaaacccgggcaggcgccagttgttgtgatttatcaggataagaatcgtccct caggcatcccggaacgctttagcggatccaacagcggcaacaccgcgaccctgaccattagcggcactcaggcggaa gacgaagcggattattattgcgctactatactggttttggttctcttgctgtgtttggcggcggcacgaagttaaccgtccta 30 - SEQ ID No 43: Amino Acid Sequence of VH of MOR-7480.1; MOR-7480.2; MOR 7483.1; MOR-7483.2 antibody 84 EVQLVQSGAEVKKPGESLRISCKGSGYSFSTYWISWVRQMPGKGLEWMGKIYPGDSY TNYSPSFQGQVTISADKSISTAYLQWSSLKASDTAMYYCARGYGIFDYWGQGTLVTVS S 5 - SEQ ID No 44: Amino Acid Sequence of Full Length Heavy Chain (IgG2) of MOR-7480.1; MOR-7480.2; MOR-7483.1; MOR-7483.2 antibody EVQLVQSGAEVKKPGESLRISCKGSGYSFSTYWISWVRQMPGKGLEWMGKIYPGDSY TNYSPSFQGQVTISADKSISTAYLQWSSLKASDTAMYYCARGYGIFDYWGQGTLVTVS Sastkgpsvfplapcsrstsestaalgcivkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtvpssnfgtqtytc 10 nvdhkpsntkvdktverkccvecppcpappvagpsvflfppkpkdtlmisrtpevtcvvvdvshedpevqfnwyvdgve vhnaktkpreeqfnstfrvvsvltvvhqdwlngkeykckvsnkglpapiektisktkgqprepqvytlppsreemtknqvslt clvkgfypsdiavewesngqpennykttppmldsdgsfflyskltvdksrwqqgnvfscsvmhealhnhytqkslslspg k 15 - SEQ ID No 45: Amino Acid Sequence of VL of MOR-7480.1 antibody SYELTQPPSVSVSPGQTASITCSGDNIGDQYAHWYQQKPGQSPVLVIYQDKN RPSGIP ERFSGSNSGNTATLTISGTQAMDEADYYCATYTGFGSLAVFGGGTKLTVL - SEQ ID No 46: Amino Acid Sequence of Full Length Light Chain of MOR-7480.1 antibody 20 SYELTQPPSVSVSPGQTASITCSGDNIGDQYAHWYQQKPGQSPVLVIYQDKNRPSGIP ERFSGSNSGNTATLTISGTQAMDEADYYCATYTGFGSLAVFGGGTKLTVLgqpkaapsvtlf ppsseelqankatvclisdfypgavtvawkadsspvkagvetttpskqsnnkyaassylsltpeqwkshrsyscqvthe gstvektvaptecs 25 - SEQ ID No 47: Nucleic Acid Sequence of VH of MOR-7480.1; MOR-7480.2; MOR-7483.1; MOR-7483.2 antibody gaggtgcaaftggttcagagcggcgcggaagtgaaaaaaccgggcgaaagcctgaggattagctgcaaaggttccgg atattccttttctacttattggatttcttgggtgcgccagatgcctgggaagggtctcgagtggatgggcaagattatccgggt 30 gatagctataccaattattctccgagctttcagggccaggtgactattagcgcggataaaagcattagcaccgcgtatcttca atggagcagcctgaaagcgagcgatacggccatgtattattgtgcgcgtggttatggtatttttgattattggggccaaggca ccctggtcaccgtctcctca - SEQ ID No 48: Nucleic Acid Sequence of VL of MOR-7480.1 antibody 85 agctacgagotgacccagccccccagcgtgtccgtgagccctggccagaccgccagcatcacctgcagcggcgacaa catcggcgaccagtacgcccactggtatcagcagaagcccggccagagccccgtgctggtgatctaccaggacaaga accggcccagcggcatccccgagcggttcagcggcagcaacagcggcaacaccgccaccctgaccatcagcggcac ccaggccatggacgaggccgactactactgcgccacctacaccggcttcggcagcctggccgtgttcggcggagggac 5 caagctgaccgtccta - SEQ ID No 49: Nucleic Acid Sequence of Full Length Heavy Chain (IgG2) of MOR-7480.1; MOR-7480.2; MOR-7483-1; MOR-7483.2 antibody gaggtgcaattggttcagagcggogoggaagtgaaaaaaccgggcgaaagcctgaggattagotgcaaaggtccgg 10 atattccttttctacftattggatttcttgggtgcgccagatgcctgggaagggtctcgagtggatgggcaagatctatccgggt gatagctataccaattattctcogagctttcagggccaggtgactattagcgcggataaaagcattagcaccgogtatttca atggagcagcctgaaagcgagcgatacggccatgtatatgtgcgcgtggttatggtatttttgattattggggccaaggca ccctggtcaccgtctcctcagcctccaccaagggcccatggtcttccccctggcgccctgctccaggagcacctccgaga gcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgctctgacc 15 agcggcgtgcacaccttcccggctgtcctacagtcotcaggactctactccctcagcagcgtagtgaccgtgccctccagc aacttcggcacccagacctacacctgcaacgtagatcacaagcccagcaacaccaaggtggacaagacagtgagcg caaatgttgtgtcgagtgcccaccgtgcccagcaccacctgtggcaggaccgtcagtcttcctcttccccccaaaacccaa ggacaccctcatgatctcccggacccctgaggtcacgtgcgtggtggtggacgtgagccacgaagaccccgaggtcca gttcaactggtacgtggacggcgtggaggtgcataatgccaagacaaagccacgggaggagcagttcaacagcacgtt 20 ccgtgtggtcagcgtcctcaccgtcgtgcaccaggactggctgaacggcaaggagtacaagtgcaaggtctccaacaaa ggcctcccagcccccatcgagaaaaccatctccaaaaccaaagggcagccccgagaaccacaggtgtacaccctgcc cccatcccgggaggagatgaccaagaaccaggtcagcctgacctgcctggtcaaaggcttctaccccagcgacatcgc cgtggagtgggagagcaatgggcagccggagaacaactacaagaccacacctccatgctggactccgacggctcctt cttcctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaacgtcttctcatgctccgtgatgcatgagg 25 ctctgcacaaccactacacacagaagagcctctccctgtctccgggtaaa - SEQ ID No 50: Nucleic Acid Sequence of Full Length Light Chain of MOR-7480.1 antibody agctacgagctgacccagccccccagcgtgtccgtgagccctggccagaccgccagcatcacctgcagcggcgacaa 30 catcggcgaccagtacgcccactggtatcagcagaagcccggccagagccccgtgctggtgatctaccaggacaaga accggcccagcggcatccccgagcggttcagcggcagcaacagcggcaacaccgccaccctgaccatcagcggcac ccaggccatggacgaggccgactactactgcgccacctacaccggcttcggcagcctggccgtgttcggcggagggac caagctgaccgtcctaggtcagcccaaggctgccccctcggtcactctgttcccaccctcctctgaggagcttcaagccaa caaggccacactggtgtgtctcataagtgacttctacccgggagccgtgacagtggcctggaaggcagatagcagcccc 86 gtcaaggcgggagtggagaccaccacaccctccaaacaaagcaacaacaagtacgcggccagcagctacctgagc ctgacgcctgagcagtggaagtcccacagaagctacagctgccaggtcacgcatgaagggagcaccgtggagaaga cagtggcccctacagaatgttca 5 - SEQ ID No 51: Amino Acid Sequence of VL of MOR-7480.2 antibody SYELTQPPSVSVSPGQTASITCSGDNIGDQYAHWYQQKPGQSPWVIYQDKNRPSGIP ERFSGSNSGNTATLTISGTQAMDEADYYCATYTGFGSLAVFGGGTKLTVL - SEQ ID No 52: Amino Acid Sequence of Full Length Light Chain of MOR-7480.2 10 antibody SYELTQPPSVSVSPGOTASITCSGDNIGDQYAHWYQQKPGQSPWVIYQDKNRPSGIP ERFSGSNSGNTATLTISGTQAMDEADYYCATYTGFGSLAVFGGGTKLTVLgqpkaapsvtlf ppsseelqankatlvclisdfypgavtvawkadsspvkagvetttpskqsnnkyaassylsltpeqwkshrsyscqvthe gstvektvaptecs 15 - SEQ ID No 53: Nucleic Acid Sequence of VL of MOR-7480.2 antibody agctacgagctgacccagccccccagcgtgtccgtgagccctggccagaccgccagcatcacctgcagcggcgacaa catcggcgaccagtacgcccactggtatcagcagaagcccggccagagccccgtggtggtgatctaccaggacaaga accggcccagcggcatccccgagcggttcagcggcagcaacagcggcaacaccgccaccctgaccatcagcggcac 20 ccaggccatggacgaggccgactactactgcgccacctacaccggcttcggcagcctggccgtgttcggcggagggac caagctgaccgtccta - SEQ ID No 54: Nucleic Acid Sequence of Full Length Light Chain of MOR-7480.2 antibody 25 agctacgagctgacccagccccccagcgtgtccgtgagccctggccagaccgccagcatcacctgcagcggcgacaa catcggcgaccagtacgcccactggtatcagcagaagcccggccagagccccgtggtggtgatctaccaggacaaga accggcccagcggcatccccgagcggttcagcggcagcaacageggcaacaccgccaccctgaccatcagcggcac ccaggccatggacgaggccgactactactgcgccacctacaccggcttcggcagcctggccgtgttcggcggagggac caagctgaccgtcctaggtcagcccaaggctgccccctcggtcactctgttcccaccctcctctgaggagcttcaagccaa 30 caaggccacactggtgtgtctcataagtgacttctacccgggagccgtgacagtggcctggaaggcagatagcagcccc gtcaaggegggagtggagaccaccacaccctccaaacaaagcaacaacaagtacgcggccagcagctacctgagc ctgacgcctgagcagtggaagtcccacagaagctacagctgccaggtcacgcatgaagggagcaccgtggagaaga cagtggcccctacagaatgttca 87 - SEQ ID No 55: L-CDR3 of MOR-7483; MOR-7483.1; MOR-7483.2 antibody STYTFVGFTTV - SEQ ID No 56: Amino Acid Sequence of VL of MOR-7483 antibody 5 DIELTQPPSVSVAPGQTARISCSGDNIGDQYAHWYQQKPGQAPWVIYQDKNRPSGIP ERFSGSNSGNTATLTISGTQAEDEADYYCSTYTFVGFTTVFGGGTKLTVL - SEQ ID No 57: Amino Acid Sequence of Full Length Light Chain of MOR-7483 antibody 10 DIELTQPPSVSVAPGQTARISCSGDNIGDQYAHWYOQKPGOAPWVIYQDKNRPSGIP ERFSGSNSGNTATLTISGTQAEDEADYYCSTYTFVGFTTVFGGGTKLTVLgqpkaapsvtlf ppsseelqankatvclisdfypgavtvawkadsspvkagvetttpskqsnnkyaassylsitpeqwkshrsyscqvthe gstvektvaptecs 15 - SEQ ID No 58: Nucleic Acid Sequence of VL of MOR-7483 antibody Gatatcgaactgacccagccgccttcagtgagcgttgcaccaggtcagaccgcgcgtatctcgtgtagcggcgataatatt ggtgatcagtatgctcattggtaccagcagaaacccgggcaggcgccagttgttgtgatttatcaggataagaatcgtcct caggcatcccggaacgctttagcggatccaacagcggcaacaccgcgaccctgaccattagcggcactcaggcggaa gacgaagcggattattattgctctacttatacttttgttggttttactactgtgtttggcggcggcacgaagttaacgtccta 20 - SEQ ID No 59: Nucleic Acid Sequence of Full Length Light Chain of MOR-7483 antibody Gatatcgaactgacccagccgccttcagtgagcgttgcaccaggtcagaccgcgcgtatctcgtgtagcggcgataatatt ggtgatcagtatgctcattggtaccagcagaaacccgggcaggcgccagttgttgtgatttatcaggataagaatcgtccct 25 caggcatcccggaacgctttagcggatccaacagcggcaacaccgcgaccctgaccattagcggcactcaggcggaa gacgaagcggattattattgctctacttatactttgttggttttactactgtgtttggcggcggcacgaagttaaccgtcctaggtc agcccaaggctgccccctcggtcactctgttcccaccctcctctgaggagcttcaagccaacaaggccacactggtgtgtct cataagtgacttctacccgggagccgtgacagtggcctggaaggcagatagcagccccgtcaaggcgggagtggaga ccaccacaccctccaaacaaagcaacaacaagtacgcggccagcagctacctgagcctgacgcctgagcagtggaa 30 gtcccacagaagctacagctgccaggtcacgcatgaagggagcaccgtggagaagacagtggcccctacagaatgttc a - SEQ ID No 60: Amino Acid Sequence Of VL of MOR-7483.1 antibody 88 SYELTQPPSVSVSPGQTASITCSGDNIGDQYAHWYQQKPGQSPVLVIYQDKNRPSGIP ERFSGSNSGNTATLTISGTQAMDEADYYCSTYTFVGFTTVFGGGTKLTVL 5 - SEQ ID No 61: Amino Acid Sequence of Full Length Light Chain of MOR-7483.1 antibody SYELTQPPSVSVSPGQTASITCSGDNIGDQYAHWYQQKPGQSPVLVIYQDKNRPSGIP ERFSGSNSGNTATLTISGTQAMDEADYYCSTYTFVGFTTVFGGGTKLTVLgqpkaapsvtlf ppsseelqankativclisdfypgavtvawkadsspvkagvetttpskqsnnkyaassylstpeqwkshrsyscqvthe 10 gstvektvaptecs - SEQ ID No 62: Nucleic Acid Sequence of VL of MOR-7483.1 antibody Agctacgagctgacccagccccccagcgtgtccgtgagccctggccagaccgccagcatcacctgcagcggcgacaa catcggcgaccagtacgoccactggtatcagcagaagccoggccagagccccgtgctggtgatctaccaggacaaga 15 accggcccagcggcatccccgagcggttcagcggcagcaacagcggcaacaccgccaccctgaccatcagcggcac ccaggccatggacgaggccgactactactgctctacttatacttttgttggttttactactgtgttcggcggagggaccaaget gaccgtccta - SEQ ID No 63: Nucleic Acid Sequence of Full Length Light Chain of MOR-7483.1 20 antibody agctacgagctgacccagccccccagcgtgtccgtgagccctggccagaccgccagcatcacctgcagcggcgacaa catcggcgaccagtacgcccactggtatcagcagaagcccggccagagccccgtgctggtgatctaccaggacaaga accggcccagcggcatccccgagcggttcagcggcagcaacagcggcaacaccgccaccctgaccatcagcggcac ccaggccatggacgaggccgactactactgctctacttatacttttgttggttttactactgtgttcggcggagggaccaaget 25 gaccgtcctaggtcagcccaaggctgccccctcggtcactctgttcccaccctcctctgaggagcttcaagccaacaaggc cacactggtgtgtctcataagtgacttctacccgggagccgtgacagtggcctggaaggcagatagcagccccgtcaagg cgggagtggagaccaccacaccctccaaacaaagcaacaacaagtacgcggccagcagctacctgagcctgacgcc tgagcagtggaagtcccacagaagctacagctgccaggtcacgcatgaagggagcaccgtggagaagacagtggcc cctacagaatgttca 30 - SEQ ID No 64: Amino Acid Sequence of Vt of MOR-7483.2 antibody SYELTQPPSVSVSPGQTASITCSGDNIGDQYAHWYQQKPGQSPVVVIYQDKNRPSGIP

ERFSGSNSGNTATLTISGTQAMDEADYYCSTYTFVGFTTVFGGGTKLTVL

89 - SEQ ID No 65: Amino Acid Sequence of Full Length Light Chain of MOR-7483.2 antibody SYELTQPPSVSVSPGQTASITCSGDNIGDQYAHWYQQKPGQSPVVVIYQDKNRPSGIP ERFSGSNSGNTATLTISGTQAMDEADYYCSTYTFVGFTTVFGGGTKLTVLgqpkaapsvtlf 5 ppsseelqankatvclisdfypgavtvawkadsspvkagvetttpskqsnnkyaassylstpeqwkshrsyscqvthe gstvektvaptecs - SEQ ID No 66: Nucleic Acid Sequence of VL of MOR-7483.2 antibody agctacgagctgacccagccccccagcgtgtccgtgagccctggccagaccgccagcatcacctgcagcggcgacaa 10 catcggegaccagtacgcccactggtatcagcagaagcccggccagagccccgtggtggtgatctaccaggacaaga accggcccagcggcatccccgagcggttcagcggcagcaacagcggcaacaccgccaccctgaccatcagcggcac ccaggccatggacgaggccgactactaotgctctacttatacttttgttggttttactactgtgttcggcggagggaccaaget gaccgtccta 15 - SEQ ID No 67: Nucleic Acid Sequence of Full Length Light Chain of MOR-7483.2 antibody agctacgagctgacccagccccccagcgtgtccgtgagccctggccagaccgccagcatcacctgcagcggcgacaa catcggcgaccagtacgcccactggtatcagcagaagcccggccagagccccgtggtggtgatctaccaggacaaga accggcccagcggcatccccgagcggttcagcggcagcaacagcggcaacaccgccaccctgaccatcagcggcac 20 ccaggccatggacgaggccgactactactgctctacttatacttttgttggttttactactgtgttcggcggagggaccaaget gaccgtcctaggtcagcccaaggctgccccctcggtcactctgttcccaccctcctctgaggagcttcaagccaacaaggc cacactggtgtgtctcataagtgacttctaccogggagccgtgacagtggcctggaaggcagatagcagccccgtcaagg cgggagtggagaccaccacaccctccaaacaaagcaacaacaagtacgcggccagcagctacctgagcctgacgcc tgagcagtggaagtcccacagaagctacagctgccaggtcacgcatgaagggagcaccgtggagaagacagtggcc 25 cctacagaatgttca - SEQ ID No 68: Amino Acid Sequence of Human 4-1BB mgnscynivatillvinfertrslqdpcsncpagtfcdnnrnqicspcppnsfssaggqrtcdicrqckgvfrtrkecsstsnae cdctpgfhclgagcsmceqdckqgqeltkkgckdccfgtfndqkrgicrpwtncsldgksvivngtkerdvvcgpspadls 30 pgassvtppaparepghspqiisffaltstallfiffltlrfsvvkrgrkkllyfkqpfmrpvqttqeedgcscrfpeeeeggce - SEQ ID No 69: Amino Acid Sequence of Human IgG1 Constant Region astkgpsvfplapsskstsggtaalgcvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslsswtvpsssigtqtyicn vnhkpsntkvdkkvepkscdkthtcppcpapeliggpsvfifppkpkdtlmisrtpevtcvvvdvshedpevkfnwyvdg 90 vevhnaktkpreeqynstyrvvsvtvlhqdwingkeykckvsnkalpapiektiskakgqprepqvytlppsreemtknq vsltclvkgfypsdiavewesngqpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealhnhytqkslsis pgk 5 - SEQ ID No 70: Nucleic Acid Sequence of Human IgG1 Constant Region GCCTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCT CTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGG TGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGG CTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTAGTGACCGTGCCCTCC 10 AGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACA CCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCA CCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAA AACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGT GGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTG 15 GAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACC GTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTA CAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCA AAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGG AGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCC 20 CAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAA GACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCA CCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCA TGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAA 25 - SEQ ID No 71: Amino Acid Sequence of Human IgG2 Constant Region astkgpsvfplapcsrstsestaalgcvkdyfpepvtvswnsgaltsgvhtfpavlqssgysissvvtvpssnfgtqtytcnv dhkpsntkvdktverkccvecppcpappvagpsvflfppkpkdtlmisrtpevtcvvvdvshedpevqfnwyvdgvevh naktkpreeqfnstfrvvsvltvvhqdwngkeykckvsnkglpapiektisktkgqprepqvytlppsreemtknqvsltcl vkgfypsdiavewesngqpennykttppmldsdgsfflyskltvdksrwqqgnvfscsvmhealhnhytqkslslspgk 30 - SEQ ID No72: Nucleic Acid Sequence of Human IgG2 Constant Region GCCTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCT CCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGG

TGACGGTGTCGTGGAACTCAGGCGCTCTGACCAGCGGCGTGCACACCTTCCCGG

91 CTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTAGTGACCGTGCCCTCC AGCAACTTCGGCACCCAGACCTACACCTGCAACGTAGATCACAAGCCCAGCAACA CCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGTCGAGTGCCCACCGTGCCC AGCACCACCTGTGGCAGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGAC 5 ACCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAGCC ACGAAGACCCCGAGGTCCAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAA TGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGC GTCCTCACCGTCGTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGG TCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGG 10 CAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACC AAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTACCCCAGCGACATCG CCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACACCTC CCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAA GAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTG 15 CACAACCACTACACACAGAAGAGCCTCTCCCTGTCTCCGGGTAAA - SEQ ID No 73: Amino Acid Sequence of Human Lambda Light Chain Constant Region gqpkaapsvtlfppsseelqankatlvclisdfypgavtvawkadsspvkagvetttpskqsnnkyaassylsltpeqwks 20 hrsyscqvthegstvektvaptecs SEQ ID No 74: Nucleic Acid Sequence of Human Lambda Light Chain Constant Region GGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC 25 TTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCC GTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACC ACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCC TGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGA AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA 30

Claims (22)

1. An isolated antibody or antigen-binding portion thereof wherein said antibody or antigen-binding portion binds human 4-1 BB extracellular domain at an epitope located within amino acid residues 115-156 of SEQ ID NO: 68. 5

2. The antibody or antigen-binding portion thereof according to Claim 1 wherein said antibody or antigen-binding portion binds human 4-1 BB with a KD of 10 nM or less for the human 4-1 BB extracellular domain as measured using a surface plasmon resonance assay.

3. The antibody or antigen-binding portion thereof according to Claim 1 or Claim 2 10 comprising a VH region amino acid sequence selected from the group consisting of SEQ ID NO:

4, SEQ ID NO: 18, SEQ ID NO: 32 and SEQ ID NO: 43; and a VL region amino acid sequence selected from the group consisting of SEQ ID NO: 9, SEQ ID NO: 23, SEQ ID NO: 37, SEQ ID NO: 45, SEQ ID NO: 51, SEQ ID NO: 56, SEQ ID NO: 60, and SEQ ID NO: 64. 15 4. A pharmaceutical composition comprising an antibody or antigen-binding portion thereof according to any one of Claims 1 to 3 and a pharmaceutically acceptable carrier.

5. A method of reducing tumor growth in a subject in need thereof, comprising administering to said subject an effective amount of an antibody or 20 antigen-binding portion thereof according to any one of Claims 1 to 3 or the pharmaceutical composition according to Claim 4.

6. A method of reducing tumor cell metastasis in a subject in need thereof, comprising administering to said subject an effective amount of an antibody or antigen-binding portion thereof according to any one of Claims 1 to 3 or the 25 pharmaceutical composition according to Claim 4.

7. A method of treating a disorder in a subject in need thereof, comprising administering to said subject a therapeutically effective amount of an antibody or antigen-binding portion thereof according to any one of Claims 1 to 3 or the pharmaceutical composition according to Claim 4. - 93

8. The method according to Claim 7 wherein said disorder is cancer.

9. The method of Claim 8 wherein said cancer is selected from the group consisting of colorectal cancer, non-Hodgkin's lymphoma, prostate cancer and melanoma.

10. A combination therapy comprising an antibody or antigen-binding portion thereof 5 according to any one of Claims 1 to 3 and a pharmaceutically acceptable carrier in combination with an immunotherapeutic agent.

11. The combination therapy of claim 10 wherein said immunotherapeutic agent is rituximab.

12. Use of an antibody or antigen-binding portion thereof according to any one of 10 Claims 1 to 3 in the preparation of a medicament for: reducing tumor growth in a subject; reducing tumor cell metastasis in a subject; or treating a disorder in a subject.

13. An isolated antibody or antigen-binding portion thereof wherein said antibody or 15 antigen-binding portion binds human 4-1 BB extracellular domain, and wherein said binding is abrogated by one or more mutations of SEQ ID NO: 68 selected from K1 15Q, C121R, R134Q, R154S, or V156A.

14. The antibody or antigen-binding portion thereof according to Claim 13 wherein the one or more mutations of SEQ ID NO: 68 comprise K1 15Q, C1 21 R, R1 34Q, R1 54S, 20 and V156A.

15. A pharmaceutical composition comprising an antibody or antigen-binding portion thereof according to Claim 13 or Claim 14 and a pharmaceutically acceptable carrier.

16. A method of reducing tumor growth in a subject in need thereof, comprising administering to the subject an effective amount of an antibody or antigen-binding 25 portion thereof according to Claim 13 or Claim 14 or the pharmaceutical composition according to Claim 15. - 94

17. A method of treating cancer in a subject in need thereof, comprising administering to the subject an effective amount of an antibody or antigen-binding portion thereof according to Claim 13 or Claim 14 or the pharmaceutical composition according to Claim 15. 5

18. The method of Claim 17 wherein said cancer is selected from the group consisting of colorectal cancer, non-Hodgkin's lymphoma, prostate cancer, and melanoma.

19. The method of Claim 17 or Claim 18 further comprising administering an immunotherapeutic agent. 10

20. The method of Claim 19 wherein said immunotherapeutic agent is rituximab.

21. Use of an antibody or antigen-binding portion thereof according to Claim 13 or Claim 14 in the preparation of a medicament for: reducing tumor growth in a subject; or treating cancer in a subject. 15

22. An isolated antibody or antigen-binding portion thereof according to Claim 1 or Claim 13; a pharmaceutical composition according to Claim 4 or Claim 15; a method according to any one of Claims 5 to 7, 16 or 17; a combination therapy according to Claim 10; or use according to Claim 12 or Claim 21, substantially as herein described with reference to any one or more of the examples but excluding comparative 20 examples.