AU2011326873B2 - IBAT inhibitors for treatment of metabolic disorders and related conditions - Google Patents

Abstract

The present invention regards certain IBAT inhibitors for the prophylaxis or treatment of a metabolic syndrome, obesity, a disorder of fatty acid metabolism, a glucose utilization disorder, or a disorder in which insulin resistance is involved such as diabetes mellitus type 1 or type 2 diabetes.

Description

1 IBAT INHIBiTORS FOR TREATMENT OF METABOUC DISORDERS AND RELATED CONDITIONS BACKGROUND OF THE INVENTION 5 Obesity is a medical condition affecting numerous humans in a number of countries throughout the world, and is associated with or induces other diseases or conditions. In particular, obesity is a seorios risk factor for diseases and conditions such as diabetes, hypertension, gallbladder disease, cancer, polycystic ovary disease and arteriosclerosis and can contribute to elevated levels of cholesterol in the blood, In addition, increased body weight due to obesity places a burden on joints causing arthritis, pain and stiffness, 10 Overeating and obesity have become a problem in the general population. Consequently there is interest in reducing food intake, losing weight, reducing weight, andlor maintaining a healthy body weight and lifestyle. W010062861 and WO 2008/058630 describe the effect of leal bile acid transport (IBAT) in the treatment of obesity and diabetes. 15 SUMMARY OF THE INVENTION The present invention regards specific iieal bile acid transport (IBAT) inhibitors with effect in the prophyaxis and treatment of metabolic syndrome, obesity, disorders of fatty acid metabolism, glucose utilization disorders, disorders in which insulin resistance is involved, diabetes mellitus, type 1 and type 2 diabetes. It also relates to compositions comprising these IBAT inhiitors, a method for treatment of the disorders and a kit comprising the substances or the compositions, It has turned out that Interruption of bile acd circulation in mice improves triglyceride metabolism and normalizes elevated plasma glucose levels. According to a first aspect of the present invention there is provided use of a compound 25 of formula II R1 00 0,11 H N O S'M O H || 0 N HO

R

2 la wherein M is -CH 2 or NH;

R

1 is H or OH; and R2 is H, CH 3 , -CH 2

CH

3 , -CH 2

CH

2

CH

3 , -CH 2

CH

2

CH

2

CH

3 , -CH(CH 3

)

2 , -CH 2

CH(CH

3

)

2 , 5 -CH(CH 3

)CH

2

CH

3 , -CH 2 OH, CH 2 0CH 3 , -CH(OH)CH 3 , -CH 2

SCH

3 , or -CH 2

CH

2

-S-CH

3 ; in the manufacture of a medicament for the prophylaxis or treatment of a glucose utilization disorder. According to a second aspect of the present invention there is provided use of a pharmaceutical composition comprising a compound of formula 11 R1 \ 0 H '0 o HO R 2 10 wherein M is -CH 2 or NH;

R

1 is H or OH; and R2 is H, CH 3 , -CH 2

CH

3 , -CH 2

CH

2

CH

3 , -CH 2

CH

2

CH

2

CH

3 , -CH(CH 3

)

2 , -CH 2

CH(CH

3

)

2 , 15 -CH(CH 3

)CH

2

CH

3 , -CH 2 OH, CH 2 0CH 3 , -CH(OH)CH 3 , -CH 2

SCH

3 , or -CH 2

CH

2

-S-CH

3 ; in the manufacture of a medicament for the prophylaxis or treatment of a glucose utilization disorder. According to a third aspect of the present invention there is provided use of a combination comprising lb (i) a compound of formula II R1 \ 0 N0 H~~ H -- aS H 0 S N HO R 2 wherein M is -CH 2 or NH; 5 R t is H or OH; and R2 is H, -CH 3 , -CH 2

CH

3 , -CH 2

CH

2

CH

3 , -CH 2

CH

2

CH

2

CH

3 , -CH(CH 3

)

2 , -CH 2

CH(CH

3

)

2 ,

-CH(CH

3

)CH

2

CH

3 , -CH 2 OH, CH 2 0CH 3 , -CH(OH)CH 3 , -CH 2

SCH

3 , or -CH 2

CH

2

-S-CH

3 ; and (ii) at least one other active substance selected from an IBAT inhibitor; an 10 enteroendocrine peptide or enhancer thereof; a dipeptidyl peptidase-IV inhibitor; a biguanidine; an incretin mimetic; a thiazolidinone; a PPAR agonist; a HMG Co-A reductase inhibitor; a bile acid binder; and a TGR5 receptor modulator; or a pharmaceutically acceptable salt of any one of said active substances, in the manufacture of a medicament for the prophylaxis or treatment of a glucose is utilization disorder. According to a fourth aspect of the present invention there is provided a method for the treatment or prophylaxis of a glucose utilization disorder, wherein a compound according to formula II R1 \ 0 N0 -0 O O R H HN0 "Sc N)C HO

R

2 1c wherein M is -CH 2 or NH;

R

1 is H or OH; and

R

2 is H, CH 3 , -CH 2

CH

3 , -CH 2

CH

2

CH

3 , -CH 2

CH

2

CH

2

CH

3 , -CH(CH 3

)

2 , -CH 2

CH(CH

3

)

2 , 5 -CH(CH 3

)CH

2

CH

3 , -CH 2 OH, CH 2 0CH 3 , -CH(OH)CH 3 , -CH 2

SCH

3 , or -CH 2

CH

2

-S-CH

3 ; is administered to a subject in need of such treatment. FIGURE LEGENDS Fig, I 10 (A) Schematic overview of vector and strategy used to target the Sic 10a2 wt allele in order to obtain a Sc0a2 nul mouse, (B) A representative immunoblot employing a specific antibody directed against the sicloa2 protein demonstrates absence of SIclOa2 protein expression in ileum of Sicl0a2-h mice, WO 2012/064268 PCT/SE2011/051337 2 Fig. 2 Disruption of the ileal BA transporter gene S/c10a2 in mice activates enzymes involved in BA synthesis and suppresses mRNA levels of the hepatic orphan nuclear receptor SHP. (A) mRNA levels 11 of CYP7A1 in livers of wt, S/c10a2+/- and S/c10a2-/- animals. (B) CYP7A1 5 enzymatic activity in pooled microsomal samples from livers of wt, S/c10a2+/- and S/c10a2-/ animals. Results shown are mean of two analyses. (C) Protein levels of CYP7A1 in livers of wt, S/c10a2+/- and S/c10a2-/- mice determined in pooled microsomal samples by immunoblot using a CYP7A1 specific antibody. 20 pg and 40 pg of protein were used per sample, respectively. The results are representative of three separate immunoblots. Note the 10 loading order of the samples. (D) Serum levels of the CYP7A1 activity marker product C4 were assayed as an indirect measure of CYP7A1 enzymatic activity in pooled serum samples of wt, S/c10a2+/- and S/c10a2-/- animals. Data show mean of two separate measurements. (E) hepatic mRNA levels of CYP8B1, and (F) hepatic SHP mRNA levels of wt,S/clOa2+/- and S/c10a2-/- mice. mRNA levels for the wt mice were normalized to 1. 15 Data are expressed as mean ± standard error (SEM) for the mRNA analysis. wt littermates (n=10), S/c10a2+/- (n=9) and S/c10a2-/- (n=10) for A-F. A p-value <0.05 is denoted *, p <0.01 is denoted **. 20 Fig. 3 Ablation of the S/c10a2 gene leads to lowered levels of plasma TGs but not plasma cholesterol with concurrent alterations of expression levels of genes involved in hepatic sterol and TG homeostasis. (A) Total plasma cholesterol and TGs from wt, Sic10a2+/- and Sic10a2-/- mice. (B) Plasma profiles of cholesterol and TGs (C) were analysed from wt, 25 S/clOa2+/- and S/clOa2-/- animals by FPLC. Lines represent means of all individuals from each group, respectively. Lipoprotein fractions are indicated, n=9-10. (D) Hepatic HMGCoA reductase mRNA levels and enzymatic activity of wt, Sic10a2+/- and Sic10a2-/- mice. HMGCoA reductase enzymatic activity was analysed from pooled hepatic microsomal samples and represents mean of two measurements. (n=9-10). (E) Hepatic mRNA levels 30 of the sterol transporters ABCG5 and ABCG8 in wt, Sic10a2+/- and Sic10a2-/- mice. (F) Hepatic mRNA levels of SREBP1c and SREBP2, in wt, S/clOa2+/- and S/clOa2-/- mice. mRNA levels in wt mice were normalized to 1. Data are expressed as mean ± standard error (SEM) for the mRNA and total plasma cholesterol and TGs analysis. wt littermates (n=10), Sic10a2+/- (n=9) and Sic10a2-/- (n=10) for A-F. A p-value < 0.05 is denoted * 35 Fig. 4 Sic10a2-/- mice display lower hepatic TG and cholesterol accumulation than wild type mice WO 2012/064268 PCT/SE2011/051337 3 concomitant with reduced expression of fatty acid synthesis genes following a sucrose-rich diet. (A) Liver TG and cholesterol content were analysed from a total of four groups; wt and S/c10a2-/- mice fed either regular chow or a sucrose-rich diet (SR) for a period of two weeks. (B) Hepatic mRNA levels of enzymes involved in fatty acid synthesis from wt or S/c10a2-/ 5 animals, as in (A). (C) SREBP1 immunoblots were performed on pooled liver cytoplasmic and nuclear protein preparations, respectively, from wt or S/c10a2-/- mice fed a regular chow or a sucrose-rich (SR) diet using an antibody specific for the N'-terminus of SREBP1. An antibody against Lamin was used as nuclear loading control. The displayed blot is representative of three separate immunoblots. Hepatic mRNA expression levels of (D) 10 Glucokinase, (GK); pyruvate kinase, (LPK); and (E) Glucose-6 phosphodehydrogenase, (G6PDH) and Malic enzyme, (ME), mRNA values in the wt group fed regular chow was normalized to 1. Data are expressed as mean ± standard error (SEM) for the mRNA, hepatic cholesterol and TG analysis. wt (n=6), S/c10a2-/- (n=5), wt + SR (n=6) and Sic10a2+/- SR (n=6) for A-D. A p-value < 0.05 is denoted * 15 Fig. 5 Interruption of BA circulation by inhibition of the Slc1Oa2 protein improves plasma glucose and TGs in ob/ob mice. Ob/ob mice were treated with a specific Slc1 0a2 protein inhibitor (Example 14), or control vehicle, (vehicle) (see experimental procedures). (A) fasting levels 20 of blood glucose and plasma insulin, (B) plasma total TGs and cholesterol. (C) Hepatic mRNA levels of FGF21 and CYP7A1. (D) Liver cholesterol and TGs (E) Distal ileum mRNA levels of FGF15, SHP, IBABP and FXR from ob/ob animals treated with a Slc1Oa2 protein inhibitor. mRNA levels of the control vehicle treated animals are normalized to 1. Data are represented as mean ± standard error (SEM). A p-value < 25 0.05 is denoted *. P < 0.01 is denoted **. Fig. 6 Pharmacological inhibition of Slcl0a2 leads to reduced hepatic SREBP1c mRNA and altered levels of glucose metabolic enzymes levels in ob/ob mice. Ob/ob mice were treated with the 30 specific Slc10a2 protein inhibitor, the compound of Example 14, (IBAT inhibitor) or control vehicle, (Controls) (see experimental procedures). (Fig. 6A) Hepatic mRNA levels of SREBP1 c, and its target genes ACC, FAS, and SCD1. (B) Hepatic mRNA levels of GK, LPK, G6Pase and PEPCK. mRNA levels of the control vehicle treated animals are normalized to 1. Data are represented as mean ± standard error (SEM). A p-value < 0.05 is denoted * 35 Fig. 7 Pharmacological inhibition of Slc10a2 induces altered activity of important signal transduction WO 2012/064268 PCT/SE2011/051337 4 pathways in ob/ob liver. The activation state of selected kinases important in glucose and lipid metabolism were investigated in individual liver protein extracts by phosphorylation site specific antibodies in SlclOa2 inhibitor treated and control ob/ob mice. (A) western blot against liver pAkt (ser 473), the same membrane was stripped and reprobed with an 5 antibody against total amount of Akt, (B) western blot against liver pMek1/2 (ser 217/221) and pErkl/2 Thr 202/ Tyr 204), the same membranes were consecutively stripped and reprobed with an antibodies against total Mekl/2 and Erkl/2, (C) western blot against liver pAmpk (Thr 172), the same membrane was stripped and reprobed with an antibody against total amount of Ampk. All membranes were finally reprobed with an antibody against 10 beta-actin. Blots are representative of four individual runs. Fig. 8 Increase of GLP-1 concentrations in human plasma after two weeks daily treatment with the compound of Example 14. Both treatment groups 15 mg (n=12) and 20 mg (n=9), 15 significantly increased p5 0.05 compared with placebo group (n=1 1). The vertical bar indicate standard deviation. Fig. 9 Percentage change of plasma glucose in patients with glucose>Upper Limit normal (ULN) 20 (Change from Baseline - End of Treatment, completer population), 10 mg of the compound of Example 14 versus placebo p= 0.013. DETAILED DESCRIPTION OF THE INVENTION 25 The invention relates to IBAT inhibitory compounds of formula (1): R6 0 O / R5 S M 4 R R N p R6 RY (Rz), (w) wherein: WO 2012/064268 PCT/SE2011/051337 5 M isCH 2 , NH One of R' and R 2 are selected from hydrogen or C 1

.

6 alkyl and the other is selected from C 1

.

6 alkyl; 5 R' and Ry are independently selected from hydrogen, hydroxy, amino, mercapto, C 1

.

6 alkyl, C 1

.

6 alkoxy, N-(C 1

.

6 alkyl)amino, NN-(C 1

.

6 alkyl) 2 amino, C1.salkylS(O)a wherein a is 0 to 2 Rz is selected from halo, nitro, cyano, hydroxy, amino, carboxy, carbamoyl, mercapto, sulphamoyl, C 1

.

6 alkyl, C 2

-

6 alkenyl, C 2

-

6 alkynyl, , C 1

.

6 alkanoyl, C 1

.

6 alkanoyloxy, 10 N-(C 1

.

6 alkyl)amino, NN-(C 1

.

6 alkyl) 2 amino, C 1

.

6 alkanoylamino, N-(C 1

.

6 alkyl)carbamoyl,

N,N-(C

1

.

6 alkyl) 2 carbamoyl, C1.salkylS(O)a wherein a is 0 to 2, C 1

.

6 alkoxycarbonyl,

N-(C

1

.

6 alkyl)sulphamoyl and NN-(C 1

.

6 alkyl) 2 sulphamoyl; v is 0-5; one of R 4 and R 5 is a group of formula (IA): A 0 N 15 R R 8 R (IA)

R

3 and R 6 and the other of R 4 and R 5 are independently selected from hydrogen, halo, nitro, cyano, hydroxy, amino, carboxy, carbamoyl, mercapto, sulphamoyl, C 1 4 alkyl, 20 C 2

-

4 alkenyl, C 2

-

4 alkynyl, C 14 alkoxy, C 1

.

4 alkanoyl, C 1

.

4 alkanoyloxy, N-(C 1

.

4 alkyl)amino,

N,N-(C

1

.

4 alkyl) 2 amino, C 1

.

4 alkanoylamino, N-(C 1

.

4 alkyl)carbamoyl, NN-(C 1

.

4 alkyl) 2 carbamoyl, C1.

4 alkylS(O)a wherein a is 0 to 2, C 1

.

4 alkoxycarbonyl, N-(C 1

.

4 alkyl)sulphamoyl and

N,N-(C

1

.

4 alkyl) 2 sulphamoyl; wherein R 3 and R 6 and the other of R 4 and R 5 may be optionally substituted on carbon by one or more R 1 6; 25 X is -0-, -N(Ra)-, -S(O)b- or -CH(Ra)-; wherein Ra is hydrogen or C 1

.

6 alkyl and b is 0-2; Ring A is aryl or heteroaryl; wherein Ring A is optionally substituted by one or more substituents selected from R 17 ;

R

7 is hydrogen, C 14 alkyl, carbocyclyl or heterocyclyl; wherein R 7 is optionally substituted by one or more substituents selected from R 18 ; 30 R 8 is hydrogen or C 14 alkyl;

R

9 is hydrogen or C 14 alkyl;

R

1 0 is hydrogen, C 14 alkyl, carbocyclyl or heterocyclyl; wherein R 10 is optionally substituted by one or more substituents selected from R 1 9

;

WO 2012/064268 PCT/SE2011/051337 6 R" is carboxy, sulpho, sulphino, phosphono, -P(O)(ORc)(ORd), -P(O)(OH)(ORc), -P(O)(OH)(Rd) or -P(O)(ORc)(Rd) wherein Rc and Rd are independently selected from

C

1

.

6 alkyl; or R" is a group of formula (IB) or (IC): R14 R 13 0 5 N (IB) (IC) wherein: Y is -N(R")-, -N(R")C(O)-, -N(R"n)C(O)(CRSR t ),N(R")C(O)-, -0-, and -S(O)a-; wherein a is 0-2, v is 1-2, Rs and R are independently selected from hydrogen or C1 4 alkyl 10 optionally substituted by R 26 and R" is hydrogen or C 14 alkyl;

R

12 is hydrogen or C 14 alkyl;

R

13 and R 14 are independently selected from hydrogen, C 1 4 alkyl, carbocyclyl or heterocyclyl; and when q is 0, R 14 may additionally be selected from hydroxy wherein R 13 and R14 may be independently optionally substituted by one or more substituents selected from 15 R20

R

15 is carboxy, sulpho, sulphino, phosphono, -P(O)(ORe)(OR'), -P(O)(OH)(ORe), -P(O)(OH)(Re) or -P(O)(ORe)(R') wherein Re and Rf are independently selected from C1.

6 alkyl; p is 1-3; wherein the values of R 13 may be the same or different; 20 q is 0-1; r is 0-3; wherein the values of R 1 4 may be the same or different; m is 0-2; wherein the values of R 1 0 may be the same or different; n is 1-3; wherein the values of R 7 may be the same or different; Ring B is a nitrogen linked heterocyclyl substituted on carbon by one group selected 25 from R 23 , and optionally additionally substituted on carbon by one or more R 24 ; and wherein if said nitrogen linked heterocyclyl contains an -NH- moiety, that nitrogen may be optionally substituted by a group selected from R 25

R

16 , R 17 and R 18 are independently selected from halo, nitro, cyano, hydroxy, amino, carboxy, carbamoyl, mercapto, sulphamoyl, C 14 alkyl, C 2

-

4 alkenyl, C 2

-

4 alkynyl, C 14 alkoxy, 30 C 14 alkanoyl, C 1

.

4 alkanoyloxy, N-(C 1

.

4 alkyl)amino, NN-(C 1

.

4 alkyl) 2 amino, C 1

.

4 alkanoylamino,

N-(C

1

.

4 alkyl)carbamoyl, NN-(C 1

.

4 alkyl) 2 carbamoyl, C1.

4 alkylS(O)a wherein a is 0 to 2,

C

1

.

4 alkoxycarbonyl, N-(C 1

.

4 alkyl)sulphamoyl and NN-(C 1

.

4 alkyl) 2 sulphamoyl; wherein R 6 , R 1 7 and R"3 may be independently optionally substituted on carbon by one or more R2; WO 2012/064268 PCT/SE2011/051337 7

R

1 9 , R 20 , R 24 and R 26 are independently selected from halo, nitro, cyano, hydroxy, amino, carboxy, carbamoyl, mercapto, sulphamoyl, C 1 4 alkyl, C 2

-

4 alkenyl, C 2

-

4 alkynyl,

C

14 alkoxy, C 14 alkanoyl, C 1

.

4 alkanoyloxy, N-(C 1

.

4 alkyl)amino, NN-(C 1

.

4 alkyl) 2 amino,

C

1

.

4 alkanoylamino, N-(C 1

.

4 alkyl)carbamoyl, NN-(C 1

.

4 alkyl) 2 carbamoyl, C1.

4 alkylS(O)a wherein 5 a is 0 to 2, C 1

.

4 alkoxycarbonyl, N-(C 1

.

4 alkyl)sulphamoyl, NN-(C 1

.

4 alkyl) 2 sulphamoyl, carbocyclyl, heterocyclyl, benzyloxycarbonylamino, sulpho, sulphino, amidino, phosphono, -P(O)(ORa)(ORb), -P(O)(OH)(ORa), -P(O)(OH)(Ra) or -P(O)(ORa)(Rb), wherein Ra and Rb are independently selected from C 1

.

6 alkyl; wherein R 1 9 , R 20 , R 24 and R 2 6 may be independently optionally substituted on carbon by one or more R22 10 R 21 and R 22 are independently selected from halo, hydroxy, cyano, carbamoyl, ureido, amino, nitro, carboxy, carbamoyl, mercapto, sulphamoyl, trifluoromethyl, trifluoromethoxy, methyl, ethyl, methoxy, ethoxy, vinyl, allyl, ethynyl, methoxycarbonyl, formyl, acetyl, formamido, acetylamino, acetoxy, methylamino, dimethylamino, N-methylcarbamoyl, N,N-dimethylcarbamoyl, methylthio, methylsulphinyl, mesyl, N-methylsulphamoyl and 15 NN-dimethylsulphamoyl;

R

23 is carboxy, sulpho, sulphino, phosphono, -P(O)(OR9)(ORh), -P(O)(OH)(OR9), -P(O)(OH)(Rg) or -P(O)(ORg)(Rh) wherein R9 and Rh are independently selected from C1.

6 alkyl;

R

25 is selected from C 1

.

6 alkyl, C 1

.

6 alkanoyl, C 1

.

6 alkylsulphonyl, C 1

.

6 alkoxycarbonyl, 20 carbamoyl, N-(C 1

.

6 alkyl)carbamoyl, NN-(C 1

.

6 alkyl)carbamoyl, benzyl, benzyloxycarbonyl, benzoyl and phenylsulphonyl; or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof for use in the prophylaxis or treatment of a metabolic syndrome, obesity, a disorder of fatty acid metabolism, a glucose utilization disorder, a disorder in which insulin resistance is 25 involved, or diabetes mellitus such as diabetes type 1 or diabetes type 2.. IBAT inhibitors as used in accordance with the present invention may reduce the activity of two major kinases namely Akt and Mekl/2. It is known that the Akt and Mekl/2 - Erkl/2 pathways are important in hepatic regulation of both glucose metabolism and lipogenesis 30 was examined. The kinase Akt has been demonstrated to be a crucial component in regulating the hepatic response to insulin and other circulating factors with capacity to favour glycolysis and lipogenesis, and inhibit gluconeogenesis upon food intake. 35 The Mekl/2 - Erkl/2 pathway is activated by the insulin receptor and the FGF receptor 4/ beta-Klotho complex, known as the FGF15 receptors.

WO 2012/064268 PCT/SE2011/051337 8 IBAT inhibitors as used in accordance with the present invention may have improved efficacy on metabolic syndrome, obesity, disorders of fatty acid metabolism, glucose utilization disorders, disorders in which insulin resistance is involved, diabetes mellitus, type 1 and type 5 2 diabetes. Further, substances as used in accordance with the invention are effective against high glucose values in serum. Consequently, the risk of hypoglycemia is low. 10 In the literature IBAT inhibitors are often referred to by different names. It is to be understood that where BAT inhibitors are referred to herein, this term also encompasses compounds known in the literature as: i) ileal apical sodium co-dependent bile acid transporter (ASBT) inhibitors; ii) bile acid transporter (BAT) inhibitors; iii) ileal sodium/bile acid cotransporter system inhibitors; iv) apical sodium-bile acid cotransporter inhibitors; v) ileal sodium 15 dependent bile acid transport inhibitors; vi) bile acid reabsorption (BARI's) inhibitors; and vii) sodium bile acid transporter (SBAT) inhibitors; where they act by inhibition of IBAT. In this specification the term "alkyl" includes both straight and branched chain alkyl groups but references to individual alkyl groups such as "propyl" are specific for the straight chain 20 version only. For example, "C 1

.

6 alkyl" includes C 14 alkyl, C 1

-

3 alkyl, propyl, isopropyl and t-butyl. However, references to individual alkyl groups such as 'propyl' are specific for the straight chained version only and references to individual branched chain alkyl groups such as 'isopropyl' are specific for the branched chain version only. A similar convention applies to other radicals, for example "phenylC 1

.

6 alkyl" would include phenylC 1

.

4 alkyl, benzyl, 1 25 phenylethyl and 2-phenylethyl. The term "halo" refers to fluoro, chloro, bromo and iodo. Where optional substituents are chosen from "one or more" groups it is to be understood that this definition includes all substituents being chosen from one of the specified groups or the substituents being chosen from two or more of the specified groups. 30 "Heteroaryl" is a totally unsaturated, mono or bicyclic ring containing 3 - 12 atoms of which at least one atom is chosen from nitrogen, sulphur or oxygen, which may, unless otherwise specified, be carbon or nitrogen linked. Preferably "heteroaryl" refers to a totally unsaturated, monocyclic ring containing 5 or 6 atoms or a bicyclic ring containing 9 or 10 atoms of which 35 at least one atom is chosen from nitrogen, sulphur or oxygen, which may, unless otherwise specified, be carbon or nitrogen linked. In another aspect of the invention, "heteroaryl" refers to a totally unsaturated, monocyclic ring containing 5 or 6 atoms or a bicyclic ring containing WO 2012/064268 PCT/SE2011/051337 9 8, 9 or 10 atoms of which at least one atom is chosen from nitrogen, sulphur or oxygen, which may, unless otherwise specified, be carbon or nitrogen linked. Examples and suitable values of the term "heteroaryl" are thienyl, isoxazolyl, imidazolyl, pyrrolyl, thiadiazolyl, isothiazolyl, triazolyl, pyranyl, indolyl, pyrimidyl, pyrazinyl, pyridazinyl, pyridyl and quinolyl. 5 Preferably the term "heteroaryl" refers to thienyl or indolyl. "Aryl" is a totally unsaturated, mono or bicyclic carbon ring that contains 3 - 12 atoms. Preferably "aryl" is a monocyclic ring containing 5 or 6 atoms or a bicyclic ring containing 9 or 10 atoms. Suitable values for "aryl" include phenyl or naphthyl. Particularly "aryl" is phenyl. 10 A "heterocyclyl" is a saturated, partially saturated or unsaturated, mono or bicyclic ring containing 3 - 12 atoms of which at least one atom is chosen from nitrogen, sulphur or oxygen, which may, unless otherwise specified, be carbon or nitrogen linked, wherein a

-CH

2 - group can optionally be replaced by a -C(O)- or a ring sulphur atom may be optionally 15 oxidised to form the S-oxides. Preferably a "heterocyclyl" is a saturated, partially saturated or unsaturated, mono or bicyclic ring containing 5 or 6 atoms of which at least one atom is chosen from nitrogen, sulphur or oxygen, which may, unless otherwise specified, be carbon or nitrogen linked, wherein a -CH 2 -group can optionally be replaced by a -C(O)- or a ring sulphur atom may be optionally oxidised to form S-oxide(s). Examples and suitable values of 20 the term "heterocyclyl" are thiazolidinyl, pyrrolidinyl, pyrrolinyl, 2-pyrrolidonyl, 2,5-dioxopyrrolidinyl, 2-benzoxazolinonyl, 1,1-dioxotetrahydrothienyl, 2,4-dioxoimidazolidinyl, 2-oxo-1,3,4-(4-triazolinyl), 2-oxazolidinonyl, 5,6-dihydrouracilyl, 1,3-benzodioxolyl, 1,2,4 oxadiazolyl, 2-azabicyclo [2.2.1] heptyl, 4-thiazolidonyl, morpholino, 2-oxotetrahydrofuranyl, tetrahyd rofu ranyl, 2,3-dihydrobenzofuranyl, benzothienyl, tetrahyd ropyranyl, piperidyl, 1-oxo 25 1,3-dihydroisoindolyl, piperazinyl, thiomorpholino, 1,1-dioxothiomorpholino, tetrahydropyranyl, 1,3-dioxolanyl, homopiperazinyl, thienyl, isoxazolyl, imidazolyl, pyrrolyl, thiadiazolyl, isothiazolyl, 1,2,4-triazolyl, 1,3,4-triazolyl, pyranyl, indolyl, pyrimidyl, thiazolyl, pyrazinyl, pyridazinyl, pyridyl, 4-pyridonyl, quinolyl and 1-isoquinolonyl. 30 A "carbocyclyl" is a saturated, partially saturated or unsaturated, mono or bicyclic carbon ring that contains 3 - 12 atoms; wherein a -CH 2 - group can optionally be replaced by a -C(O)-. Preferably "carbocyclyl" is a monocyclic ring containing 5 or 6 atoms or a bicyclic ring containing 9 or 10 atoms. Suitable values for "carbocyclyl" include cyclopropyl, cyclobutyl, 1 oxocyclopentyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, phenyl, naphthyl, 35 tetralinyl, indanyl or 1-oxoindanyl. Particularly "carbocyclyl" is cyclopropyl, cyclobutyl, 1 -oxocyclopentyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, phenyl or 1 oxoindanyl.

WO 2012/064268 PCT/SE2011/051337 10 An example of "C 1

.

6 alkanoyloxy" and "C 1

.

4 alkanoyloxy" is acetoxy. Examples of

"C

1

.

6 alkoxycarbonyl" and "C 1

.

4 alkoxycarbonyl" include methoxycarbonyl, ethoxycarbonyl, n- and t-butoxycarbonyl. Examples of "C 1

.

6 alkoxy" and "C 1

.

4 alkoxy" include methoxy, ethoxy 5 and propoxy. Examples of "C 1

.

6 alkanoylamino" and "C 1

.

4 alkanoylamino" include formamido, acetamido and propionylamino. Examples of "C1.salkylS(O)a wherein a is 0 to 2" and "C1. 4 alkylS(O)a wherein a is 0 to 2" include methylthio, ethylthio, methylsulphinyl, ethylsulphinyl, mesyl and ethylsulphonyl. Examples of "C 1

.

6 alkanoyl" and "C 1

.

4 alkanoyl" include C 1

-

3 alkanoyl, propionyl and acetyl. Examples of "N-(C 1

.

6 alkyl)amino" and "N-(C 1

.

4 alkyl)amino" include 10 methylamino and ethylamino. Examples of "N,N-(C 1

.

6 alkyl) 2 amino" and "NN-(C 1 . 4 alkyl) 2 amino" include di-N-methylamino, di-(N-ethyl)amino and N-ethyl-N-methylamino. Examples of "C 2

-

6 alkenyl" and "C 2

-

4 alkenyl" are vinyl, allyl and 1-propenyl. Examples of "C2 6 alkynyl" and "C 2

-

4 alkynyl" are ethynyl, 1-propynyl and 2-propynyl. Examples of "N-(C 1 . 6 alkyl)sulphamoyl" and "N-(C 1

.

4 alkyl)sulphamoyl" are N-(C 1

-

3 alkyl)sulphamoyl, N 15 (methyl)sulphamoyl and N-(ethyl)sulphamoyl. Examples of "N-(C 1

.

6 alkyl) 2 sulphamoy" and "N-4alkyl) 2 sulphamoyl" are NN-(dimethyl)sulphamoyl and N-(methyl)-N-(ethyl)sulphamoyl. Examples of "N-(C 1

.

6 alkyl)carbamoyl" and "N-(C 1

.

4 alkyl)carbamoyl" are methylaminocarbonyl and ethylaminocarbonyl. Examples of "NN-(C 1

.

6 alkyl) 2 carbamoyl" and "NN-(C 1 . 4 alkyl) 2 carbamoyl" are dimethylaminocarbonyl and methylethylaminocarbonyl. Examples of 20 "C 1

.

6 alkoxycarbonylamino" are ethoxycarbonylamino and t-butoxy-carbonylamino. Examples of "N'-(C 1

.

6 alkyl)ureido" are N'-methylureido and N'-ethylureido. Examples of "N-(C 1 . 6 alkyl)ureido are N-methylureido and N-ethylureido. Examples of "N',N'-(C 1

.

6 alkyl) 2 ureido are N',N'-dimethylureido and N'-methyl-N'-ethylureido. Examples of "N'-(C 1

.

6 alkyl)-N-(C 1 . 6 alkyl)ureido are N'-methyl-N-methylureido and N'-propyl-N-methylureido. Examples of 25 "N',N'-(C 1

.

6 alkyl) 2

-N-(C

1

.

6 alkyl)ureido are N',N'-dimethyl-N-methylureido and N'-methyl-N' ethyl-N-propylureido. A suitable pharmaceutically acceptable salt of a compound of the invention is, for example, an acid-addition salt of a compound of the invention which is sufficiently basic, for example, 30 an acid-addition salt with, for example, an inorganic or organic acid, for example hydrochloric, hydrobromic, sulphuric, phosphoric, trifluoroacetic, citric or maleic acid. In addition a suitable pharmaceutically acceptable salt of a compound of the invention which is sufficiently acidic is an alkali metal salt, for example a sodium or potassium salt, an 35 alkaline earth metal salt, for example a calcium or magnesium salt, an ammonium salt or a salt with an organic base which affords a physiologically-acceptable cation, for example a WO 2012/064268 PCT/SE2011/051337 11 salt with methylamine, dimethylamine, trimethylamine, piperidine, morpholine or tris-(2 hydroxyethyl) amine. A prodrug of any compound mentioned herein as an IBAT inhibitor or a compound for use in 5 combination therewith is a drug which is broken down in the human or animal body to give the compound. Examples of pro-drugs include in vivo hydrolysable esters and in vivo hydrolysable amides of a compound of the formula (1). 10 An in vivo hydrolysable ester of a compound of the formula (1) containing carboxy or hydroxy group is, for example, a pharmaceutically acceptable ester which is hydrolysed in the human or animal body to produce the parent acid or alcohol. Suitable pharmaceutically acceptable esters for carboxy include C 1

.

6 alkoxymethyl esters for example methoxymethyl, C1. 15 6 alkanoyloxymethyl esters for example pivaloyloxymethyl, phthalidyl esters, C3 8 cycloalkoxycarbonyloxyC 1

.

6 alkyl esters for example 1-cyclohexylcarbonyloxyethyl;1,3 dioxolen-2-onylm ethyl esters for example 5-methyl-1,3-dioxol en-2-onylm ethyl; and C1. 6 alkoxycarbonyloxyethyl esters for example 1-methoxy-carbonyloxyethyl and may be formed at any carboxy group in the compounds of this invention. 20 An in vivo hydrolysable ester of a compound of the formula (1) containing a hydroxy group includes inorganic esters such as phosphate esters and a-acyloxyalkyl ethers and related compounds which as a result of the in vivo hydrolysis of the ester breakdown to give the parent hydroxy group. Examples of a-acyloxyalkyl ethers include acetoxymethoxy and 25 2,2-dimethylpropionyloxy-methoxy. A selection of in vivo hydrolysable ester forming groups for hydroxy include alkanoyl, benzoyl, phenylacetyl and substituted benzoyl and phenylacetyl, alkoxycarbonyl (to give alkyl carbonate esters), dialkylcarbamoyl and N(dialkylaminoethyl)-N-alkylcarbamoy (to give carbamates), dialkylaminoacetyl and carboxyacetyl. Examples of substituents on benzoyl include morpholino and piperazino 30 linked from a ring nitrogen atom via a methylene group to the 3-or 4-position of the benzoyl ring. A suitable value for an in vivo hydrolysable amide of a compound of the formula (1) containing a carboxy group is, for example, a N-C 1

.

6 alkyl or NN-di-C 1

.

6 alkyl amide such as 35 N-methyl, N-ethyl, N-propyl, NN-dimethyl, N-ethyl-N-methyl or NN-diethyl amide.

WO 2012/064268 PCT/SE2011/051337 12 It is also to be understood that certain compounds of the formula (1) can exist in solvated as well as unsolvated forms such as, for example, hydrated forms. It is to be understood that the invention encompasses all such solvated forms which possess IBAT inhibitory activity. 5 Preferred values of R', R 2 , R 3 , R 4 , R 5 and R 6 are as follows. Such values may be used where appropriate with any of the definitions, claims or embodiments defined hereinbefore or hereinafter. Preferably R 1 and R 2 are independently selected from C 1 4 alkyl. 10 More preferably R 1 and R 2 are independently selected from ethyl or butyl. More preferably R 1 and R 2 are independently selected from ethyl, propyl or butyl. In one aspect of the invention particularly R 1 and R 2 are both butyl. In a further aspect of the invention particularly R 1 and R 2 are both propyl. In another aspect of the invention particularly one of R 1 and R 2 is ethyl and the other is butyl. 15 Preferably R' and RY are independently selected from hydrogen or C 1

.

6 alkyl. More preferably R' and RY are both hydrogen. Preferably Rz is selected from halo, amino, C 1

.

6 alkyl, C 1

.

6 alkoxycarbonylamino or

N'-(C

1

.

6 alkyl)ureido. 20 More preferably Rz is selected from chloro, amino, t-butyl, t-butoxycarbonylamino or N'-(t-butyl)ureido. Preferably v is 0 or 1. 25 In one aspect of the invention, more preferably v is 0. In one aspect of the invention, more preferably v is 1. In one aspect of the invention preferably R 4 is a group of formula (IA) (as depicted above). In another aspect of the invention preferably R' is a group of formula (IA) (as depicted above). 30 Preferably R 3 and R 6 are hydrogen. Preferably the other of R 4 and R 5 that is not the group of formula (IA) is selected from halo,

C

14 alkoxy or C1 4 alkylS(O)a wherein a is 0 to 2; wherein that R 4 or R 5 may be optionally substituted on carbon by one or more R 16 ; wherein R 16 is independently selected from 35 hydroxy and NN-(C 1

.

4 alkyl) 2 amino.

WO 2012/064268 PCT/SE2011/051337 13 More preferably the other of R 4 and R 5 that is not the group of formula (IA) is selected from bromo, methoxy, isopropoxy, methylthio, ethylthio, isopropylthio or mesyl; wherein that R 4 or

R

5 may be optionally substituted on carbon by one or more R 16 ; wherein R 16 is independently selected from hydroxy and NN-dimethylamino. 5 Particularly the other of R 4 and R 5 that is not the group of formula (IA) is selected from bromo, methoxy, isopropoxy, methylthio, ethylthio, isopropylthio, 2-hydroxyethylthio, 2-(NN dimethylamino) ethylthio or mesyl. 10 More particularly the other of R 4 and R 5 that is not the group of formula (IA) is methylthio. Preferably the other of R 4 and R 5 that is not the group of formula (IA) is selected from hydrogen, halo, C 14 alkoxy or C1.

4 alkylS(O)a wherein a is 0 to 2; wherein that R 4 or R 5 may be optionally substituted on carbon by one or more R 16 ; wherein R 16 is independently selected from hydroxy, carboxy and NN-(C 1

.

4 alkyl) 2 amino. 15 More preferably the other of R 4 and R 5 that is not the group of formula (IA) is selected from hydrogen, bromo, methoxy, isopropoxy, methylthio, ethylthio, isopropylthio or mesyl; wherein that R 4 or R 5 may be optionally substituted on carbon by one or more R 16 ; wherein R 16 is independently selected from hydroxy, carboxy and NN-dimethylamino. 20 Particularly the other of R 4 and R 5 that is not the group of formula (IA) is selected from hydrogen, bromo, methoxy, isopropoxy, methylthio, carboxymethylthio, ethylthio, isopropylthio, 2-hydroxyethylthio, 2-(NN-dimethylamino) ethylthio or mesyl. In another aspect of the invention, more preferably the other of R 4 and R 5 that is not the 25 group of formula (IA) is selected from hydrogen, chloro, bromo, methoxy, isopropoxy, methylthio, ethylthio or isopropylthio; wherein that R 4 or R 5 may be optionally substituted on carbon by one or more R 16 ; wherein R 16 is independently selected from hydroxy, carboxy and N,N-dimethylamino. 30 In another aspect of the invention, particularly the other of R 4 and R 5 that is not the group of formula (IA) is selected from hydrogen, chloro, bromo, methoxy, isopropoxy, methylthio, carboxymethylthio, ethylthio, isopropylthio, 2-hydroxyethylthio or 2-(NN-dimethylamino) ethylthio. 35 In another aspect of the invention, more particularly the other of R 4 and R 5 that is not the group of formula (IA) is bromo or chloro.

WO 2012/064268 PCT/SE2011/051337 14 In another aspect of the invention, more particularly the other of R 4 and R 5 that is not the group of formula (IA) is methoxy. In one aspect of the invention, preferably Ring A is aryl. 5 In another aspect of the invention, preferably Ring A is heteroaryl. When Ring A is aryl, preferably Ring A is phenyl. When Ring A is heteroaryl, preferably Ring A is thienyl or indolyl. Preferably Ring A is aryl or heteroaryl; wherein Ring A is optionally substituted by one or more substituents selected from R 17 ; wherein R 17 is selected from halo, hydroxy or Cl 4alkyl; 10 wherein R 17 may be optionally substituted on carbon by one or more R 21 ; wherein R 21 is selected from halo. Preferably X is-O. More preferably Ring A is phenyl, thienyl or indolyl; wherein Ring A is optionally substituted 15 by one or more substituents selected from halo, hydroxy or trifluoromethyl. Particularly Ring A is selected from phenyl, 4-hydroxyphenyl, thien-2-yl, 4 trifluoromethylphenyl, 3-hydroxyphenyl, 2-fluorophenyl, 2,3-dihydroxyphenyl or indol-3-yl. More particularly Ring A is phenyl. 20 In another aspect of the invention, preferably Ring A is aryl or heteroaryl; wherein Ring A is optionally substituted by one or more substituents selected from R 17 ; wherein R 17 is selected from halo, hydroxy, C 1 4 alkyl or C 14 alkoxy; wherein R 17 may be optionally substituted on carbon by one or more R 21 ; wherein R 21 is selected from halo. 25 In another aspect of the invention, more preferably Ring A is phenyl, thienyl or indolyl; wherein Ring A is optionally substituted by one or more substituents selected from halo, hydroxy, methoxy or trifluoromethyl. In another aspect of the invention, particularly Ring A is selected from phenyl, 30 4-hydroxyphenyl, 4-methoxyphenyl, thien-2-yl, 4-trifluoromethylphenyl, 3-hydroxyphenyl, 2-fluorophenyl, 2,3-dihydroxyphenyl or indol-3-yl. In a further aspect of the invention, particularly Ring A is selected from phenyl, 4-hydroxyphenyl, 4-methoxyphenyl, thien-2-yl, 4-trifluoromethylphenyl, 3-hydroxyphenyl, 2-fluorophenyl, 4-fluorophenyl, 2,3-dihydroxyphenyl or indol-3-yl. 35 Preferably R 7 is hydrogen, C 1 4 alkyl or carbocyclyl. More preferably R 7 is hydrogen, methyl or phenyl.

WO 2012/064268 PCT/SE2011/051337 15 Particularly R 7 is hydrogen. In one aspect of the invention, preferably R 8 is hydrogen. In another aspect of the invention, preferably R 8 is C 14 alkyl. In another aspect of the invention, more preferably R 8 is hydrogen or methyl. 5 In one aspect of the invention, preferably R 9 is hydrogen. In another aspect of the invention, preferably R 9 is C 14 alkyl. In another aspect of the invention, more preferably R 9 is hydrogen or methyl. Preferably R 10 is hydrogen. In one aspect of the invention, preferably R 1 1 is carboxy, sulpho, sulphino, phosphono, 10 -P(O)(OR)(ORd), -P (O)(OH)(OR), -P(O)(OH)(Rd) or -P(O)(ORc) (Rd) wherein Rc and Rd are independently selected from C 1

.

6 alkyl. In another aspect of the invention, preferably R 11 is a group of formula (IB) (as depicted above). 15 Preferably R 1 1 is carboxy, -P(O)(OH)(OR) or a group of formula (IB) (as depicted above). More preferably R 11 is carboxy, -P(O)(OH)(OEt) or a group of formula (IB) (as depicted above). 20 In another aspect of the invention, preferably R 1 1 is carboxy, sulpho, -P(O)(OH)(OR) wherein Rc is selected from C 1 4 alkyl or a group of formula (IB) (as depicted above). Preferably Y is -NH- or -NHC (0)-. More preferably Y is -NHC (0)-. In one aspect of the invention, preferably R 12 is hydrogen. 25 In another aspect of the invention, preferably R 12 is C 14 alkyl. In another aspect of the invention, more preferably R 12 is hydrogen or methyl. Preferably R 13 is hydrogen, C 1 4 alkyl or carbocyclyl; wherein R 13 is optionally substituted by one or more substituents selected from R 20 ; wherein R 2 0 is hydroxy. More preferably R 13 is hydrogen, methyl or phenyl; wherein R 13 is optionally substituted by 30 one or more substituents selected from R 20 ; wherein R 2 0 is hydroxy. Particularly R 13 is hydrogen, hydroxymethyl or phenyl. More particularly R 13 is hydrogen or hydroxymethyl. 35 In another aspect of the invention, preferably R 13 is hydrogen, C 14 alkyl or carbocyclyl; wherein R 13 is optionally substituted by one or more substituents selected from R 2 0 ; wherein WO 2012/064268 PCT/SE2011/051337 16

R

2 0 is hydroxy, carboxy, carbocyclyl or amino; wherein R 20 may be optionally substituted on carbon by one or more R 22 ; R 2 2 is hydroxy. In another aspect of the invention, more preferably R 13 is hydrogen, methyl, ethyl, butyl or 5 phenyl; wherein R 13 is optionally substituted by one or more substituents selected from R 2 0 wherein R 2 0 is hydroxy, carboxy, phenyl or amino; wherein R 2 0 may be optionally substituted on carbon by one or more R 2 2 ; R 2 2 is hydroxy. In another aspect of the invention, particularly R 13 is hydrogen, hydroxymethyl, 4-aminobutyl, 10 2-carboxyethyl, 4-hydroxybenzyl or phenyl. In a further aspect of the invention, preferably R 13 is hydrogen, C 1 4 alkyl or carbocyclyl; wherein R 13 is optionally substituted by one or more substituents selected from R 2 0 ; wherein

R

2 0 is hydroxy, carboxy, carbocyclyl, heterocyclyl or amino; wherein R 20 may be optionally 15 substituted on carbon by one or more R 2 2 ; R 22 is hydroxy. In a further aspect of the invention, more preferably R 13 is hydrogen, methyl, ethyl, butyl or phenyl; wherein R 13 is optionally substituted by one or more substituents selected from R 2 0 wherein R 2 0 is hydroxy, carboxy, phenyl, imidazolyl or amino; wherein R 20 may be optionally 20 substituted on carbon by one or more R 2 2 ; R 22 is hydroxy. In a further aspect of the invention, particularly R 13 is hydrogen, hydroxymethyl, 4 aminobutyl, 2-carboxyethyl, 4-hydroxybenzyl, imidazol-5-ylm ethyl or phenyl. 25 In another further aspect of the invention, preferably R 13 is hydrogen, C 14 alkyl, carbocyclyl or

R

2 3 ; wherein R 13 is optionally substituted by one or more substituents selected from R 2 0 ; wherein R 2 0 is hydroxy, C 14 alkylS (0) a wherein a is 0, C 14 alkoxy, amino, carbocyclyl, heterocyclyl or mercapto; wherein R 2 0 may be independently optionally substituted on carbon by one or more R 2 2 ; R 22 is selected from hydroxy; and R 2 3 is carboxy. 30 In another further aspect of the invention, more preferably R 13 is hydrogen, methyl, ethyl, butyl or phenyl or R 2 3 ; wherein R 13 is optionally substituted by one or more substituents selected from R 20 ; wherein R 2 0 is hydroxy, methylthio, methoxy, amino, imidazolyl or mercapto; wherein R 2 0 may be independently optionally substituted on carbon by one or 35 more R 22 ; R 2 2 is selected from hydroxy; and R 23 is carboxy.

WO 2012/064268 PCT/SE2011/051337 17 In another further aspect of the invention, particularly R 13 is hydrogen, carboxy, hydroxymethyl, mercaptomethyl, methoxymethyl, methylthiomethyl, 2-methylthioethyl, 4-aminobutyl, 4-hydroxybenzyl, imidazol-5-ylmethyl or phenyl. 5 In another aspect more particularly R 13 is methylthiomethyl, methylsulphinylmethyl or methylsulphonylmethyl. Preferably R 14 is hydrogen. 10 In another aspect of the invention, preferably R14 is selected from hydrogen, C 1 4 alkyl or carbocyclyl; wherein said C 1 4 alkyl or carbocyclyl may be optionally substituted by one or more substituents selected from R 2 0 ; and R 2 0 is hydroxy. In another aspect of the invention, more preferably R 14 is selected from hydrogen, methyl or 15 phenyl; wherein said methyl or phenyl may be optionally substituted by one or more substituents selected from R 2 0 ; and R 2 0 is hydroxy. In another aspect of the invention, particularly R 14 is hydrogen, phenyl or hydroxymethyl. Particularly R 15 is carboxy or sulpho. 20 In one aspect of the invention, more particularly R 15 is carboxy. In another aspect of the invention, more particularly R 15 is sulpho. Preferably R 15 is carboxy, sulpho,-P(O)(ORe) (OR), -P(O)(OH)(ORe), -P(O)(OH)(Re) or 25 -P(O)(ORe)(R) wherein Re and Rf are independently selected from C 1 4 alkyl. More preferably R 15 is carboxy, sulpho, -P(O)(ORe)(OR), -P(O)(OH)(ORe), -P(O)(OH)(Re) or P(O)(ORe)(R) wherein Re and Rf are independently selected from methyl or ethyl. Preferably R 15 is carboxy, sulpho, -P(O)(OEt)(OEt), -P(O)(OH)(OEt), -P(O)(OH)(Me) or -P (O)(OEt)(Me). 30 Preferably R 15 is carboxy, sulpho, phosphono, -P(O)(ORe)(OR), -P(O)(OH)(ORe), -P(O)(OH) (Re) or -P(O)(ORe)(R) wherein Re and Rf are independently selected from C 14 alkyl or R 15 is a group of formula (IC) (as depicted above). 35 More preferably R 15 is carboxy, sulpho, phosphono,-P(O)(ORe)(OR), -P(O)(OH)(ORe), -P(O)(OH)(Re) or -P(O)(ORe)(R) wherein Re and Rf are independently selected from methyl or ethyl or R 15 is a group of formula (IC) (as depicted above).

WO 2012/064268 PCT/SE2011/051337 18 Preferably R 15 is carboxy, sulpho, phosphono, -P(O)(OEt)(OEt), -P (O)(Ot-Bu)(Ot-Bu), -P(O)(OH)(OEt), -P (O)(OH)(Me) or -P(O)(OEt)(Me) or R 15 is a group of formula (IC) (as depicted above). 5 In one aspect of the invention, preferably R 15 is carboxy. In another aspect of the invention, preferably R 15 is sulpho. In another aspect of the invention, preferably R 15 is -P(O)(OH)(OEt). In another aspect of the invention, preferably R 15 is -P(O)(OH)(Me). 10 In another aspect of the invention, preferably R 15 is -P(O)(OEt)(Me). In one aspect of the invention, preferably R 24 is hydrogen. In another aspect of the invention, preferably R 2 4 is C 14 alkyl. Preferably R 25 is hydrogen. 15 Preferably R 26 is carboxy. Preferably p is 1 or 2; wherein the values of R 13 may be the same or different. In one aspect of the invention, more preferably p is 1. In another aspect of the invention, more preferably p is 2; wherein the values of R 13 may be 20 the same or different. In a further aspect of the invention, more preferably p is 3; wherein the values of R 13 may be the same or different. In one aspect of the invention, preferably q is 0. In a further aspect of the invention, preferably q is 1. 25 In one aspect of the invention, preferably r is 0. In one aspect of the invention, more preferably r is 1. In another aspect of the invention, more preferably r is 2; wherein the values of R 14 may be the same or different. In a further aspect of the invention, more preferably r is 3; wherein the values of R 14 may be 30 the same or different. Preferably m is 0. In another aspect of the invention, preferably m is 0 or 1. Preferably n is 1. 35 In another aspect of the invention, preferably n is 1 or 2. Preferably z is 1.

WO 2012/064268 PCT/SE2011/051337 19 The group of formula (IA) wherein R 7 is hydrogen, methyl or phenyl, n is 1, Ring A is phenyl, thienyl or indolyl; wherein Ring A is optionally substituted by one or more substituents selected from halo, hydroxy or trifluoromethyl, m is 0 and R 9 is carboxy, -P(O)(OH)(ORc) or a group of formula (IB). 5 The group of formula (IA) wherein: X is -0-. Ring A is phenyl, thienyl or indolyl; wherein Ring A is optionally substituted by one or more substituents selected from halo, hydroxy, methoxy or trifluoromethyl;

R

7 is hydrogen, methyl or phenyl;

R

8 is hydrogen or methyl; 10 R 9 is hydrogen or methyl;

R

1 0 is hydrogen; m is 0-2 wherein the values of R 1 0 may be the same or different; and R 11 is carboxy, -P(O)(OH)(OEt) or a group of formula (IB) (as depicted in claim 1); The group of formula (IB) wherein R 10 is hydrogen, hydroxymethyl or phenyl, p is 1 or 2; wherein the values of R 1 0 may 15 be the same or different and R 11 is carboxy or sulpho. The group of formula (IB) wherein:

R

12 is hydrogen or methyl;

R

13 is hydrogen, methyl, ethyl, butyl or phenyl or R 23 ; wherein R 13 is optionally substituted by one or more substituents selected from R 20 ; R 20 is hydroxy, methylthio, methoxy, amino, 20 imidazolyl or mercapto; wherein R 20 may be independently optionally substituted on carbon by one or more hydroxy; R 23 is carboxy; Y is -NH- or -NHC (0)-; R 14 is selected from hydrogen, methyl or phenyl; wherein said methyl or phenyl may be optionally substituted by one or more substituents selected from hydroxy; R 15 is carboxy, sulpho, phosphono, P(O)(ORe)(OR), -P(O)(OH)(ORe), -P(O)(OH)(Re) or -P(O)(ORe)(R') wherein Re and Rf are 25 independently selected from methyl or ethyl or R 15 is a group of formula (IC) (as depicted in claim 1); p is 1-3 wherein the values of R 13 may be the same or different; q is 0-1; and r is 0-3 wherein the values of R 14 may be the same or different; 30 The group of formula (IC) wherein

R

2 4 is hydrogen;

R

2 5 is hydrogen;

R

2 6 is carboxy; and z is 1; 35 or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof.

WO 2012/064268 PCT/SE2011/051337 20 Therefore in a further aspect of the invention, there is provided a compound of formula (1) as depicted above wherein:

R

1 and R 2 are independently selected from ethyl or butyl;

R

3 and R 6 are hydrogen; 5 R4 is selected from halo, C 14 alkoxy or C1 4 alkylS(O)a wherein a is 0 to 2; wherein that R 4 may be optionally substituted on carbon by one or more R 1 6 ; wherein R 16 is independently selected from hydroxy and NN-(C 1

.

4 alkyl) 2 amino;

R

5 is a group of formula (IA); Ring A is aryl or heteroaryl; wherein Ring A is optionally substituted by one or more 10 substituents selected from R 17 ; wherein

R

17 is selected from halo, hydroxy or C 14 alkyl; wherein R 17 may be optionally substituted on carbon by one or more R 21 ; wherein

R

2 1 is selected from halo;

R

7 is hydrogen, C 14 alkyl or carbocyclyl; 15 R 11 is carboxy, -P(O)(OH)(ORc) or a group of formula (IB) (as depicted above);

R

13 is hydrogen, C 1 4 alkyl or carbocyclyl; wherein R 13 is optionally substituted by one or more substituents selected from R 2 0 ; wherein

R

2 0 is hydroxy;

R

15 is carboxy or sulpho; 20 p is 1 or 2; wherein the values of R 13 may be the same or different; m is 0; and n is 1; or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof. 25 Therefore in an additional aspect of the invention, there is provided a compound of formula (1) as depicted above wherein:

R

1 and R 2 are both butyl or one of R 1 and R 2 is ethyl and the other is butyl;

R

4 is methylthio;

R

5 is a group of formula (IA) (as depicted above); 30 R 3 and R 6 are hydrogen; Ring A is phenyl;

R

7 is hydrogen;

R

11 is a group of formula (IB) (as depicted above);

R

13 is hydrogen or hydroxymethyl; 35 R 5 is carboxy or sulpho; p is 1 or 2; wherein the values of R 13 may be the same or different; m is 0; WO 2012/064268 PCT/SE2011/051337 21 n is 1; or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof. Therefore in an additional further aspect of the invention, there is provided a compound of 5 formula (1) as depicted above wherein:

R

1 and R 2 are independently selected from ethyl or butyl;

R

3 and R 6 are hydrogen;

R

4 is selected from halo, C 14 alkoxy or C1 4 alkylS(O)a wherein a is 0 to 2; wherein that R 4 may be optionally substituted on carbon by one or more R 1 6 ; wherein R 16 is independently 10 selected from hydroxy and NN-(C 1

.

4 alkyl) 2 amino;

R

5 is a group of formula (IA); Ring A is aryl or heteroaryl; wherein Ring A is optionally substituted by one or more substituents selected from R 17 ;

R

7 is hydrogen, C 1 4 alkyl or carbocyclyl; 15 R 8 is hydrogen or methyl;

R

9 is hydrogen or methyl;

R

11 is carboxy, -P(O)(OH)(ORc) or a group of formula (IB) (as depicted above); X is -NH- or -NHC(O)-;

R

12 is hydrogen or methyl; 20 R 13 is hydrogen, C 1 4 alkyl or carbocyclyl; wherein R 13 is optionally substituted by one or more substituents selected from R 20

R

14 is hydrogen;

R

15 is carboxy or sulpho;

R

17 is selected from halo, hydroxy, C 14 alkyl or C 1 4 alkoxy; wherein R 17 may be optionally 25 substituted on carbon by one or more R 2 1

R

20 is hydroxy, carboxy, carbocyclyl or amino; wherein R 20 may be optionally substituted on carbon by one or more R22

R

21 is selected from halo;

R

22 is hydroxy; 30 p is 1-3; wherein the values of R 13 may be the same or different; q is 0-1; r is 0-3; wherein the values of R 14 may be the same or different; and wherein if q is 1, r is not 0; m is 0-2; and 35 n is 1-3; or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof.

WO 2012/064268 PCT/SE2011/051337 22 Therefore in another additional further aspect of the invention, there is provided a compound of formula (1) as depicted above wherein:

R

1 and R 2 are independently selected from C 14 alkyl; R' and Ry are both hydrogen; 5 Rz is selected from halo, amino, C 1

.

6 alkyl, C 1

.

6 alkoxycarbonylamino or N'-(C 1

.

6 alkyl)ureido; v is 0 or 1;

R

3 and R 6 are hydrogen; one of R 4 and R 5 is a group of formula (IA) (as depicted above) and the other is selected from hydrogen, halo, C 14 alkoxy or C1 4 alkylS(O)a wherein a is 0 to 2; wherein that R 4 or R 5 10 may be optionally substituted on carbon by one or more R 16 ; wherein R 16 is independently selected from hydroxy, carboxy and NN-(C 1

.

4 alkyl)2amino; X is -0

R

7 is hydrogen, methyl or phenyl;

R

8 is hydrogen or methyl; 15 Ring A is aryl or heteroaryl; wherein Ring A is optionally substituted by one or more substituents selected from R 17 ; wherein R 17 is selected from halo, hydroxy, C 1 4 alkyl or

C

14 alkoxy; wherein R 17 may be optionally substituted on carbon by one or more R 21 ; wherein

R

21 is selected from halo;

R

9 is hydrogen or methyl; 20 R 1 0 is hydrogen;

R

11 is carboxy, -P(O)(OH)(ORc) wherein Rc is selected from C 1 4 alkyl or a group of formula (IB) (as depicted above);

R

12 is hydrogen or methyl; Y is -NH- or -NHC(O)-; 25 R 13 is hydrogen, C 14 alkyl, carbocyclyl or R 23 ; wherein R 13 is optionally substituted by one or more substituents selected from R 2 0 ; wherein R 20 is hydroxy, C1 4 alkylS(O)a wherein a is 0,

C

14 alkoxy, amino, carbocyclyl, heterocyclyl or mercapto; wherein R 2 0 may be independently optionally substituted on carbon by one or more R 22 ; R 2 2 is selected from hydroxy; and R 2 3 is carboxy; 30 R 14 is selected from hydrogen, C 1 4 alkyl or carbocyclyl; wherein said C 14 alkyl or carbocyclyl may be optionally substituted by one or more substituents selected from R 2 0 ; and R 2 0 is hydroxy;

R

15 is carboxy, sulpho, phosphono, -P(O)(ORe)(OR'), -P(O)(OH)(ORe), -P(O)(OH)(Re) or -P(O)(ORe)(R) wherein Re and Rf are independently selected from C 1 4 alkyl or R 15 is a group 35 of formula (IC) (as depicted above);

R

2 4 is hydrogen;

R

2 5 is hydrogen; WO 2012/064268 PCT/SE2011/051337 23

R

2 6 is carboxy; p is 1-3; wherein the values of R 13 may be the same or different; q is 0-1; r is 0-3; wherein the values of R 14 may be the same or different; 5 m is 0-2; wherein the values of R 1 0 may be the same or different; n is 1-2; wherein the values of R 7 may be the same or different; z is 0-1; wherein the values of R 25 may be the same or different; or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof. 10 An aspect of the invention is a compound of formula || R1 \ /0 H H S N s HO R2 Formula II 15 wherein M is CH 2 or NH

R

1 is H or hydroxy

R

2 is H, CH 3 , -CH 2

CH

3 , -CH 2

CH

2

CH

3 , -CH 2

CH

2

CH

2

CH

3 , -CH(CH 3

)

2 , -CH 2

CH(CH

3

)

2 , CH(CH 3

)CH

2

CH

3 , -CH 2 OH, -CH 2 0CH 3 , -CH(OH)CH 3 , -CH 2

SCH

3 , -CH 2

CH

2

SCH

3 ; 20 for use in the prophylaxis or treatment of a metabolic syndrome. In one aspect of the invention the metabolic syndrome is a disorder of fatty acid metabolism. In yet an aspect of the invention the disorder of fatty acid metabolism is obesity. 25 An aspect of the invention is a compound of formula || WO 2012/064268 PCT/SE2011/051337 24 R1 \ 0 N0 H o HO R2 Formula II 5 wherein M is CH 2 or NH R' is H or hydroxy

R

2 is H, CH 3 , -CH 2

CH

3 , -CH 2

CH

2

CH

3 , -CH 2

CH

2

CH

2

CH

3 , -CH(CH 3

)

2 , -CH 2

CH(CH

3

)

2 , CH(CH 3

)CH

2

CH

3 , -CH 2 OH, -CH 2 0CH 3 , -CH(OH)CH 3 , -CH 2

SCH

3 , -CH 2

CH

2

SCH

3 ; 10 for use in the prophylaxis or treatment of a glucose utilization disorder. In one aspect of the invention, the glucose utilization disorder is a disorder in which insulin resistance is involved. 15 In one aspect of the invention, the glucose utilization disorder is diabetes mellitus type 1. In one aspect of the invention, the glucose utilization disorder is diabetes mellitus type 2. 20 Examples of useful substances according to the invention are: 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-(carboxymethyl)carbamoyl] benzyl} carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5-benzothiadiazepine, 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8- (N-{(R)-a-[N'-((S)-1-carboxyethyl) carbamoyl] benzyl} carbamoylmethoxy)-2,3,4,5-tetrahydro-1,5-benzothiazepine, 25 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1-carboxypropyl) carbamoyl]benzyl}carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5-benzothiadiazepine, 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((R)-1-carboxy-2 methylthioethyl)carbamoyl]benzyl}carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5 benzothiadiazepine, 30 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1-carboxypropyl) carbamoyl] 4-hydroxybenzyl}carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5-benzothiadiazepine, WO 2012/064268 PCT/SE2011/051337 25 1,1 -Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((R)-1 -carboxy-2-methylthio ethyl)carbamoyl]-4-hyd roxybenzyl}carbamoylmethoxy)-2,3,4,5-tetrahyd ro- 1,2,5 benzothiadiazepine, 1,1 -Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1 -carboxy-2 5 methylpropyl)carbamoyl]benzyl}carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5 benzothiadiazepine, 1,1 -Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1 -carboxy-2-(R) hydroxypropyl)carbamoyl]-4-hyd roxybenzyl}carbamoylmethoxy)-2,3,4,5-tetrahyd ro- 1,2,5 benzothiadiazepine, 10 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1-carboxybutyl) carbamoyl] 4-hydroxybenzyl}carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5-benzothiadiazepine, 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1-carboxyethyl) carbamoyl]benzyl}carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5-benzothiadiazepine, 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N'-((S)-1-carboxypropyl)carbamoyl] 15 4-hydroxybenzyl}carbamoylmethoxy)-2,3,4,5-tetrahydro-1,5-benzothiazepine, 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1-carboxyethyl)carbamoyl]-4 hydroxybenzyl}carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5-benzothiadiazepine, 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1-carboxy-2 m ethylpropyl)carbamoyl]-4-hyd roxybenzyl}carbamoylmethoxy)-2,3,4,5-tetrahyd ro- 1,2,5 20 benzothiadiazepine and 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{ (R)-1'-phenyl-1'-[N'-(carboxymethyl) carbamoyl]methyl}carbamoylmethoxy)-2,3,4,5-tetrahydro-1,5-benzothiazepine. In another aspect of the invention, preferred compounds of the invention are any one of the 25 examples or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof. Compounds of formula (1) or formula (II) may have chiral centres and/or geometric isomeric centres (E-and Z-isomers), and it is to be understood that the invention encompasses the 30 use of all such optical, diastereoisomers and geometric isomers that possess IBAT inhibitory activity in accordance with the invention. The invention relates to any and all tautomeric forms of the compounds of the formula (1) that possess IBAT inhibitory activity. The invention also relates all possible isomers of the compounds of the invention such as, 35 optical and/or geometrical, pure or as a mixture, in all proportions, of the said compounds of formulas I and || and those specifically mentioned and the possible tautomeric forms.

WO 2012/064268 PCT/SE2011/051337 26 In certain embodiments, compounds described herein have one or more chiral centres. As such, all stereoisomers are envisioned herein. In various embodiments, compounds described herein are present in optically active or racemic forms. It is to be understood that the compounds of the present invention encompasses racemic, optically-active, 5 regioisomeric and stereoisomeric forms, or combinations thereof that possess the therapeutically useful properties described herein. Preparation of optically active forms is achieve in any suitable manner, including by way of non-limiting example, by resolution of the racemic form by recrystallization techniques, by synthesis from optically-active starting materials, by chiral synthesis, or by chromatographic separation using a chiral stationary 10 phase. In some embodiments mixtures of one or more isomer is utilized as the therapeutic compound described herein. In certain embodiments, compounds described herein contain one or more chiral centres. These compounds are prepared by any means, including enantioselective synthesis and/or separation of a mixture of enantiomers and/or diastereomers. Resolution of compounds and isomers thereof is achieved by any means 15 including, by way of non-limiting example, chemical processes, enzymatic processes, fractional crystallization, distillation, chromatography, and the like. In another aspect of the invention, preferred compounds of the invention are any one of the Examples or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug 20 thereof. An aspect of the invention is a composition comprising a compound according to the invention for use in prophylaxis and treatment of metabolic syndrome, obesity, disorders of fatty acid metabolism, glucose utilization disorders, disorders in which insulin resistance is 25 involved, diabetes mellitus, type 1 and type 2 diabetes. It also relates to the use of a substance or a composition according to the invention for the preparation of a medicine or a pharmaceutical composition for use in the treatment of a metabolic syndrome, obesity, disorders of fatty acid metabolism, glucose utilization 30 disorders, disorders in which insulin resistance is involved, diabetes mellitus, type 1 and type 2 diabetes. According to the invention the IBAT inhibitors with formula I and || above may be combined with least one other active substance. The active substance may be another substance with 35 IBAT inhibitory effect.

WO 2012/064268 PCT/SE2011/051337 27 Combination Therapy with Other Active Substances In certain instances, provided herein are combination compositions and/or therapies comprising any compound described herein and another active substance, which may be 5 a L-cell endocrine peptide enhancer. In one embodiment, the L-cell endocrine peptide enhancer is a PYY enhancer. Enhanced secretion of PYY may provide a reduction of hunger. The L-cell endocrine peptide enhancer may be an oxyntomodulin enhancer. In some instances, the enhanced secretion of 10 oxyntomodulin inhibits meal-stimulated gastric secretion. Another useful L-cell endocrine peptide enhancer is a GLP-1 enhancer. Examples of GLP-1 enhancers are GLP-1, a GLP-1 secretion enhancer, a GLP-1 degradation inhibitor and the like, or a combination thereof. In certain instances, enhanced GLP-1 concentration provides 15 a reduction in food intake and/or a reduction in gastric emptying in human subjects. The L-cell endocrine peptide enhancer may also be a GLP-2 enhancer, such as a GLP-2, a GLP-2 secretion enhancer, a GLP-2 degradation inhibitor, etc. or a combination thereof. According to one embodiment, enhanced GLP-2 secretion inhibits gastric emptying and 20 reduces intestinal permeability and/or the enhanced GLP-2 secretion inhibits gastric acid secretion. Enhanced GLP-2 secretion may also reduce or prevent inflammation in the gastrointestinal tract (gastrointestinal enteritis) and/or regenerate and/or heal injury to gastrointestinal tissues (e.g., radiation enteritis). 25 In some instances, the other active substance modulates bile acid receptors in the gastrointestinal lumen and or other organs. In some embodiments, the other active substance substantially or partially agonizes bile acid receptors (e.g., TGR5 receptors or Farnesoid-X receptors) in the gastrointestinal tract. The other active substance In some embodiments, the additional therapeutic agent may be a bile acid analogue. In certain 30 instances the additional therapeutic agent is a TGR5 agonist. Administration of a TGR5 agonist in combination with any of the compounds described herein may enhance the secretion of enteroendocrine peptides from L-cells. TGR5 modulators (e.g., agonists) include, and are not limited to, the compounds described in, WO 2008/091540, WO 2008/067219 and U.S. Apple. No. 2008/0221161. 35 WO 2012/064268 PCT/SE2011/051337 28 In some embodiments, other active substance is a biguanide. A biguanide may reduce blood and/or plasma glucose levels. Examples of biguanides include and are not limited to metformin, phenformin, buformin, proguanil or the like. 5 In some embodiments, the other active substances are selected from enteroendocrine peptides. They may reverse insulin resistance and lower blood and/or plasma glucose levels. Examples of enteroendocrine peptides include but are not limited to GLP-1 or GLP-1 analogues such as Taspoglutide.RTM. (Ipsen) or the like. 10 In another embodiment, the other active substance is a thiazolidinedione. Thiazolidinediones may reverse insulin resistance and lower blood and/or plasma glucose levels. Examples of thiazolidinediones include and are not limited to Rosiglitazone (Avandia), Pioglitazone (Actos), Troglitazone (Rezulin), MCC-555, rivoglitazone, ciglitazone or the like. 15 In some embodiments, the additional therapeutic agent is an incretin mimetic, which could mimic augments pancreas response to ingestion of food, in some instances, administration of an incretin mimetic in combination with any of the compounds described herein lowers blood and/or plasma glucose levels. Examples of incretin mimetics include but are not limited to exenatide (Byetta.RTM.). 20 One currently used therapy for the treatment of diabetes is a subcutaneous injection of exenatide (Byetta.RTM.). In some embodiments, an oral combination of an IBAT inhibitor and a DPP-IV inhibitor is equally or more effective than an injection of exenatide in reducing plasma glucose levels. In some embodiments, an oral combination of an IBAT inhibitor and a 25 DPP-IV inhibitor reduces or eliminates discomfort associated with injections of glucose lowering medications. According to the invention an IBAT inhibitor may be used together with a DPP-IV Inhibitor. In some embodiments, the other active substance inhibits degradation of L-cell 30 enteroendocrine peptides. In certain embodiments, the other active substance is a DPP-IV inhibitor. Administration of an IBAT inhibitor to an individual in need thereof may enhance the secretion of GLP-1. Administration of a DPP-IV inhibitor in combination with the IBAT inhibitor may reduce or inhibit degradation of GLP-1 thereby prolonging the therapeutic benefit of enhanced levels of GLP-1. In some instances, administration of an IBAT inhibitor 35 reduces weight of an individual. Thus, administration of an IBAT inhibitor in combination with a DPP-IV inhibitor may reduce weight of an individual.

WO 2012/064268 PCT/SE2011/051337 29 DPP-IV inhibitors may be selected from (2S)-1-{2-[(3-hydroxy-1-adamantyl)amino]acetyl} pyrrolidine-2-carbonitrile (vildagliptin), (3R)-3-amino-1-[9-(trifluoromethyl)-1,4,7,8 tetrazabicyclo[4.3.0]nona-6,8- -dien-4-yl]-4-(2,4,5-trifluorophenyl)butan-1 -one (sitagliptin), (1S,3S,5S)-2-[(2S)-2-amino-2-(3-hydroxy-1-adamantyl)acetyl]-2-azabicyclo[3.1.0]hexane-3 5 carbonitrile (saxagliptin), and 2-({6-[(3R)-3-aminopiperidin-1-yl]-3-methyl-2,4-dioxo-3,4 dihydropyrimidi- n-1(2H)-yl}methyl)benzonitrile (alogliptin). Another therapy that is current standard of care for the treatment of diabetes is a combination of metformin and sitagliptin (Janumet.RTM.). At doses of 0,3 - 300 mg/kg 10 metformin in combination with 30 mg/kg of sitagliption, induce reduction in plasma glucose concentrations from 3 hours till about 6 hours post-dose. In some embodiments, a combination of an IBAT inhibitor and sitagliptin maintains reduced plasma glucose concentrations for a longer duration of time compared to a combination of metformin and sitagliptin. In some instances IBAT inhibitor therapy eliminates side effects associated with 15 metformin therapy and/or DPP-IV inhibitor therapy. In some embodiments of any of the methods described herein, administration of an ASBT inhibitor described herein in combination with a DPP-IV inhibitor increases the level of GLP-1 in the blood and/or plasma of an individual by from about 1.5 times to about 30 times 20 compared to the level of GLP-1 in the blood and/or plasma of the individual prior to administration of the IBAT inhibitor in combination with the DPP-IV inhibitor. In some instances, an increase in GLP-1 level of from about 2 times to about 3 times following the administration of an ASBT inhibitor described herein in combination with a DPP 25 IV inhibitor compared to the level of GLP-1 in the blood and/or plasma of the individual prior to administration of the IBAT inhibitor in combination with the DPP-IV inhibitor is associated with an anti-diabetic effect and/or with reduction in food intake and/or induction of satiety and/or weight loss. 30 In some embodiments of any of the methods described herein, administration of an IBAT inhibitor in combination with a DPP-IV inhibitor reduces blood and/or plasma sugar levels for a longer period of time (e.g., at least 24 hours) compared to reduction in blood and/or plasma sugar levels upon administration of metformin in combination with a DPP-IV inhibitor. 35 In some embodiments of any of the methods described herein, administration of a single dose of a compound in accordance with the invention in combination with a DPP-IV inhibitor sustains reduced blood and/or plasma sugar levels for at least 6 hours, at least 12 hours, at WO 2012/064268 PCT/SE2011/051337 30 least 14 hours, at least 16 hours, at least 18 hours, at least 20 hours, at least 24 hours, at least 30 hours, at least 36 hours or at least 48 hours compared to reduction in blood and/or plasma sugar levels upon administration of a single dose of metformin in combination with a DPP-IV inhibitor. 5 According to one embodiment, administration of an IBAT inhibitor in combination with a DPP IV inhibitor may reduces blood and/or plasma sugar levels by at least 20% compared to blood and/or plasma sugar levels prior to administration of the IBAT inhibitor in combination with a DPP-IV inhibitor. 10 In some embodiments of any of the methods described herein, administration of an IBAT INHIBITOR in combination with a DPP-IV inhibitor reduces blood and/or plasma sugar levels by at least 20%, e.g. at least 30%, such as at least 40%, e.g. at least 50% compared to blood and/or plasma sugar levels prior to administration of the IBAT inhibitor in combination with a 15 DPP-IV inhibitor. According to one embodiment of the invention, administration of an IBAT inhibitor in combination with a DPP-IV inhibitor results in higher levels of GLP-1 in blood and/or plasma of an individual compared to levels of GLP-1 in blood and/or plasma of a normal individual. In 20 some embodiments of any of the methods described herein, administration of an IBAT inhibitor in combination with a DPP-IV inhibitor results in higher levels of GLP-1 in blood and/or plasma of an individual compared to levels of GLP-1 in blood and/or plasma of an individual undergoing therapy with metformin and/or a DPP-IV inhibitor. 25 In some embodiments, an IBAT inhibitor is administered in combination with a DPP-IV inhibitor and/or a biliary shunt. Biliary shunts may be selected from the shunts described in WO 2007/0050628, which is incorporated herein by reference. A biliary shunt may move bile acid to the distal ileum and/or the rectum and/or the colon thereby increasing the concentration of bile acids in the vicinity of L-cells present in the distal portion of the 30 gastrointestinal tract. Such an increase in the concentration of bile acids in the vicinity of L cells increases in some instances the secretion of GLP-1 from L-cells thereby inducing satiety and/or reduction in hunger and/or weight loss and/or reduction in plasma glucose levels or any combination thereof. 35 The other active substance and the IBAT inhibitor are used such that the combination is present in a therapeutically effective amount. For example an IBAT inhibitor and the other active substance (e.g., a DPP-IV inhibitor) are each is used in a therapeutically effective WO 2012/064268 PCT/SE2011/051337 31 amount. When an additive or synergistic effect is present, they can each be used in a subclinical therapeutically effective amount. In some embodiments, the use of a combination of an IBAT inhibitor and any other active 5 ingredient as described herein encompasses combinations where the IBAT inhibitor or the other active ingredient is present in a therapeutically effective amount, and the other is present in a subclinical therapeutically effective amount, provided that the combined use is therapeutically effective owing to their additive or synergistic effects. As used herein, the term "additive effect" describes the combined effect of two (or more) pharmaceutically active 10 agents that is equal to the sum of the effect of each agent given alone. A synergistic effect is one in which the combined effect of two (or more) pharmaceutically active agents is greater than the sum of the effect of each agent given alone. Any suitable combination of an ASBTI with one or more of the aforementioned other active ingredients and optionally with one or more other pharmacologically active substances is contemplated as being within the scope 15 of the methods described herein. Methods for experimentally determining therapeutically-effective dosages of drugs and other agents for use in combination treatment regimens are described in the literature. 20 The compounds may be administered concurrently e.g., simultaneously, essentially simultaneously or within the same treatment protocol or sequentially, depending upon the nature of the disease, disorder, or condition, the condition of the individual, and the actual choice of compounds used. 25 The multiple therapeutic agents are optionally administered in any order or simultaneously. If simultaneously, the multiple therapeutic agents are optionally provided in a single, unified form or in multiple forms, e.g. as a single pill or as two separate pills. In certain instances, one of the therapeutic agents is optionally given in multiple doses. In other instances, both are optionally given as multiple doses. If not simultaneous, the timing between the multiple 30 doses may be, e.g., from more than a couple of days to less than four weeks. In addition, the combination methods, compositions and formulations are not to be limited to the use of only two agents. The use of multiple therapeutic combinations is also envisioned including two or more of the active substances described herein. 35 The active substances in a combination therapy described herein may be provided in a combined dosage form or in separate dosage forms intended for substantially simultaneous administration. In some embodiments, the active compounds are administered sequentially, WO 2012/064268 PCT/SE2011/051337 32 with either therapeutic compound being administered by a regimen using a two-step administration. In some embodiments, two-step administration regimen calls for sequential administration of the active agents or spaced-apart administration of the separate active agents. In certain embodiments, the time period between the multiple administration steps 5 varies, by way of non-limiting example, from a few minutes to several hours, depending upon the properties of each pharmaceutical agent, such as potency, solubility, bioavailability, plasma half-life and kinetic profile of the pharmaceutical agent. In certain embodiments, a dosage regimen to treat, prevent, or ameliorate the condition(s), is 10 modified depending on e.g. the disorder from which the subject suffers, as well as the age, weight, sex, diet, and medical condition of the subject. Thus, in various embodiments, the dosage regimen actually employed may differ from the dosage regimens set forth herein. In certain embodiments, IBAT inhibitor compounds described herein are combined with or one or more of: insulin, insulin-mimetics, incretin mimetics, GLP-1 or analogues thereof, 15 GLP-2 or analogues thereof, oxyntomodulin, PYY, DPP-IV inhibitors, or TGR5 modulators in any combination. The invention also regards IBAT inhibitor compounds described herein in combination with at least one bile acid binder e.g. a resin such as cholestyramine, cholestipol and colesevelam. 20 Bile acid binders (bile acid sequestrants, resins) The following bile acid binders may be used according to the invention. Cholestyramine a hydrophilic polyacrylic quaternary ammonium anion exchange resin, which is known to be effective in reducing blood cholesterol levels. Cholestyramine, and various 25 compositions including cholestyramine, are described, for example, in British Pat Nos. 929,391 and 1,286, 949; and U. S. Patent Nos. 3,383, 281; 3,308, 020; 3,769, 399; 3,846, 541; 3,974, 272; 4,172, 120; 4,252, 790; 4,340, 585; 4,814, 354; 4,874, 744; 4,895, 723; 5,695, 749; and 6,066, 336. Cholestyramine is commercially available from Novopharm, USA Inc (Questrans Light), Upsher-Smith (PREVALITE (D) and Apothecon. As used herein, 30 "cholestyramine" includes any such composition comprising cholestyramine, or pharmaceutically acceptable salts thereof. These are also called Questrans TM Questran Light Questrans Light (cholestyramine) is a non-absorbable anion binding resin FDA approved for the treatment of hypercholesterolemia. 35 An amine polymer having a first substituent, bound to a first amine of the amine polymer, that includes a hydrophobic aliphatic moiety, and a second substituent, bound to a second amine WO 2012/064268 PCT/SE2011/051337 33 of the amine polymer that includes an aliphatic quaternary amine-containing moiety as described in USP 5,693,675 and 5,607,669. The salt of an alkylated and crosslinked polymer comprising the reaction product of: (a) one 5 or more crosslinked polymers, or salts and copolymers thereof having a repeat unit selected from the group consisting of: (NR-CH 2

CH

2 )n (2) and (NR-CH 2

CH

2

-NR-CH

2

CH

2

-NR

CH

2

CHOH-CH

2 )n (3) where n is a positive integer and each R, independently, is H or a Cl C8 alkyl group; (b) at least one aliphatic alkylating agent, said reaction product characterized in that: (i) at least some of the nitrogen atoms in said repeat units unreacted with said 10 alkylating agent; (ii) less than 10 mol percent of the nitrogen atoms in said repeat units reacting with said alkylating agent forming quaternary ammonium units; and (iii) a fixed positive charge and one or more counter ions, such as Colesevelam and colesevelam hydrochloride. 15 Suitable bile acid binders for such a combination therapy are resins, such as cholestyramine and cholestipol. One advantage is that the dose of bile acid binder might be kept lower than the therapeutic dose for treatment of cholesterolaemia in single treatment comprising solely a bile acid binder. By a low dose of bile acid binder any possible side effects caused by poor tolerance of the patient to the therapeutic dose could also be avoided. 20 Another useful bile acid binder is a water insoluble non-toxic polymeric amine having a molecular weight in excess of 3,000, having the property of binding at least 30% of the available glycocholic acid within 5 minutes when exposed to an aqueous solution of an equal weight of said acid, having a polymer skeleton inert to digestive enzymes, and having a 25 water content greater than 65% after equilibration with air at 100% relative humidity, e.g., cholestipol described in USP 3,383,281. In a further aspect of the invention a suitable bile acid binder is one of cholestyramine, cholestipol or colesevelam. 30 A preferred aspect of the present invention is the use of colesevelam as the bile acid binder. According to an additional further aspect of the present invention there is provided a combination treatment comprising the administration of an effective amount of an IBAT 35 inhibitor compound or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof, and a bile acid binder, wherein the formulation is designed to deliver the bile WO 2012/064268 PCT/SE2011/051337 34 acid binder in the colon, with the simultaneous, sequential or separate administration one or more of the following agents selected from: Statins 5 In another aspect of the invention, an IBAT inhibitor compound e.g. a compound of formula (1) or (II) or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof, may be administered in association with an HMG Co-A reductase inhibitor, or pharmaceutically acceptable salts, solvates, solvates of such salts or prodrugs thereof. Suitable HMG Co-A reductase inhibitors, pharmaceutically acceptable salts, solvates, 10 solvates of such salts or prodrugs thereof are statins well known in the art. Particular statins are fluvastatin, lovastatin, pravastatin, simvastatin, atorvastatin, cerivastatin, bervastatin, dalvastatin, mevastatin and (E)-7- [4- (4- fluorophenyl)-6-isopropyl-2- [methyl (methylsulphonyl) amino] pyrimidin-5-yl] (3R, 5S)-3,5-dihydroxyhept-6-enoic acid (rosuvastatin), or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a 15 prodrug thereof. A particular statin is atorvastatin, or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof. A more particular statin is atorvastatin calcium salt. A further particular statin is (E)-7- [4- (4- fluorophenyl)-6-isopropyl-2- [methyl (methylsulphonyl) amino] pyrimidin-5-yl] (3R, 5S)-3,5-dihydroxyhept-6-enoic acid (rosuvastatin), or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a 20 prodrug thereof. Other particular statin are rosuvastatin calcium salt and pitavastatin. In an additional aspect of the invention, the compound of formula (1), or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof may be administered in association with an HMG Co-A reductase inhibitor, or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof, and/or a bile acid binder thereby avoiding 25 a possible risk of excess of bile acids in colon caused by the inhibition of the ileal bile acid transport system. An excess of bile acids in the visceral contents may cause diarrhoea. Thus, the present invention also provides a treatment of a possible side effect such as diarrhoea in patients during therapy comprising the compound of formula (1), or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof. 30 An HMG CoA-reductase inhibitor, or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof will by its action decrease the endogenous cholesterol available for the bile acid synthesis and have an additive effect in combination with the compound of formula (1), or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof on lipid lowering. 35 WO 2012/064268 PCT/SE2011/051337 35 A CETP (cholesteryl ester transfer protein) inhibitor, for example those referenced and described in WO 00/38725 page 7 line 22-page 10, line 17 which are incorporated herein by reference; A cholesterol absorption antagonist for example azetidinones such as SCH 58235 and those 5 described in US 5,767,115 which are incorporated herein by reference; MTP (microsomal transfer protein) inhibitor for example those described in Science, 282,751 54,1998 which are incorporated herein by reference; A fibric acid derivative; for example clofibrate, gemfibrozil, fenofibrate, ciprofibrate and bezafibrate; 10 A nicotinic acid derivative, for example, nicotinic acid (niacin), acipimox and niceritrol; A phytosterol compound for example stanols; Probucol ; An anti-obesity compound for example orlistat (EP 129,748) and sibutramine (GB 2,184,122 and US 4,929,629); 15 An antihypertensive compound for example an angiotensin converting enzyme (ACE) inhibitor, an angiotensin || receptor antagonist, an adrenergic blocker, an alpha adrenergic blocker, a beta adrenergic blocker, a mixed alpha/beta adrenergic blocker, an adrenergic stimulant, calcium channel blocker, a diuretic or a vasodilator; Insulin; 20 Sulphonylureas including glibenclamide and/or tolbutamide. Acarbose; or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof, optionally together with a pharmaceutically acceptable diluent or carrier to a warm-blooded animal, such as man in need of such therapeutic treatment. 25 ACE inhibitors Particular ACE inhibitors or pharmaceutically acceptable salts, solvates, solvate of such salts or a prodrugs thereof, including active metabolites, which can be used in combination with a compound of formula (1) include but are not limited to, the following compounds: alacepril, 30 alatriopril, altiopril calcium, ancovenin, benazepril, benazepril hydrochloride, benazeprilat, benzoylcaptopril, captopril, captopril-cysteine, captopril- glutathione, ceranapril, ceranopril, ceronapril, cilazapril, cilazaprilat, delapril, delapril-diacid, enalapril, enalaprilat, enapril, epicaptopril, foroxymithine, fosfenopril, fosenopril, fosenopril sodium, fosinopril, fosinopril sodium, fosinoprilat, fosinoprilic acid, glycopril, hemorphin-4, idrapril, imidapril, indolapril, 35 indolaprilat, libenzapril, lisinopril, lyciumin A, lyciumin B, mixanpril, moexipril, moexiprilat, moveltipril, muracein A, muracein B, muracein C, pentopril, perindopril, perindoprilat, pivalopril, pivopril, quinapril, quinapril hydrochloride, quinaprilat, ramipril, ramiprilat, spirapril, WO 2012/064268 PCT/SE2011/051337 36 spirapril hydrochloride, spiraprilat, spiropril, spiropril hydrochloride, temocapril, temocapril hydrochloride, teprotide, trandolapril, trandolaprilat, utibapril, zabicipril, zabiciprilat, zofenopril and zofenoprilat. Preferred ACE inhibitors for use in the present invention are ramipril, ramiprilat, lisinopril, enalapril and enalaprilat. More preferred ACE inhibitors for uses in the 5 present invention are ramipril and ramiprilat. Angiotensin II antagonists Preferred angiotensin || antagonists, pharmaceutically acceptable salts, solvates, solvate of such salts or a prodrugs thereof for use in combination with a compound of formula (1) 10 include, but are not limited to, compounds: candesartan, candesartan cilexetil, losartan, valsartan, irbesartan, tasosartan, telmisartan and eprosartan. Particularly preferred angiotensin || antagonists or pharmaceutically acceptable derivatives thereof for use in the present invention are candesartan and candesartan cilexetil. 15 PPAR alpha and/or gamma and/or delta agonists or a pharmaceutically acceptable salt thereof. In another aspect of the invention, the IBAT inhibitor compound, or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof, may be administered in 20 association with a PPAR alpha and/or gamma and/or delta agonist, or pharmaceutically acceptable salts, solvates, solvates of such salts or prodrugs thereof. Suitable PPAR alpha and/or gamma and/or delta agonists, pharmaceutically acceptable salts, solvates, solvates of such salts or prodrugs thereof are well known in the art. These include the compounds described in WO 01/12187, WO 01/12612, WO 99/62870, WO 99/62872, WO 99/62871, WO 25 98/57941, WO 01/40170, J Med Chem, 1996, 39,665, Expert Opinion on Therapeutic Patents, 10 (5), 623-634 (in particular the compounds described in the patent applications listed on page 634) and J Med Chem, 2000,43,527, which are all incorporated herein by reference. Particularly a PPAR alpha and/or gamma agonist refers to WY-14643, clofibrate, fenofibrate, bezafibrate, GW 9578, troglitazone, pioglitazone, rosiglitazone, eglitazone, 30 proglitazone, BRL-49634, KRP-297, JTT-501, SB 213068, GW 1929, GW 7845, GW 0207, L-796449, L-165041 and GW 2433. Particularly a PPAR alpha and/or gamma agonist refers to (S)-2-ethoxy-3- [4- (2- {4 methanesulphonyloxyphenyl} ethoxy) phenyl] propanoic acid and pharmaceutically 35 acceptable salts thereof.

WO 2012/064268 PCT/SE2011/051337 37 Antidiabetics, hypoglycemic active ingredients, cholesterol absorption inhibitors, PPAR delta agonists, fibrates, MTP inhibitors, bile acid absorption inhibitors, polymeric bile acid adsorbents, LDL receptor inducers, ACAT inhibitors, antioxidants, lipoprotein lipase inhibitors, ATP-citrate lyase inhibitors, squalene synthetase inhibitors, lipoprotein(a) 5 antagonists, HM74A receptor agonists, lipase inhibitors, insulins, sulfonylureas, biguanides, meglitinides, thiazolidinediones, alpha-glucosidase inhibitors, active ingredients which act on the ATP-dependent potassium channel of the beta cells, glycogen phosphorylase inhibitors, glucagon receptor antagonists, activators of glucokinase, inhibitors of gluconeogenesis, inhibitors of fructose-1,6-bisphosphatase, modulators of glucose transporter 4, inhibitors of 10 glutamine-fructose-6-phosphate amidotransferase, inhibitors of dipeptidylpeptidase IV, inhibitors of 1 1-beta-hydroxysteroid dehydrogenase 1, inhibitors of protein tyrosine phosphatase 1 B, modulators of the sodium-dependent glucose transporter 1 or 2, modulators of GPR40, inhibitors of hormone-sensitive lipase, inhibitors of acetyl-CoA carboxylase, inhibitors of phosphoenolpyruvate carboxykinase, inhibitors of glycogen 15 synthase kinase-3 beta, inhibitors of protein kinase C beta, endothelin-A receptor antagonists, inhibitors of I kappaB kinase, modulators of the glucocorticoid receptor, CART agonists, NPY agonists, MC4 agonists, orexin agonists, H3 agonists, TNF agonists, CRF agonists, CRF BP antagonists, urocortin agonists, beta 3 agonists, CB1 receptor antagonists, MSH (melanocyte-stimulating hormone) agonists, CCK agonists, serotonin 20 reuptake inhibitors, mixed serotoninergic and noradrenergic compounds, 5HT agonists, bombesin agonists, galanin antagonists, growth hormones, growth hormone-releasing compounds, TRH agonists, uncoupling protein 2 or 3 modulators, leptin agonists, DA agonists (bromocriptine, Doprexin), lipase/amylase inhibitors, PPAR modulators, RXR modulators or TR-beta-agonists or amphetamines. 25 Examples of PPAR delta agonists are GW-501516 (501516, GSK-516, GW-516, GW-1516;a peroxisome proliferator-activated receptor (PPAR)-delta agonist, and several other compounds developed from GW-501516, including GI-262570, GW-0072, GW-7845 and GW-7647. 30 According to one embodiment the IBAT inhibitor may be combined with one or more of Atreleuton( 5-LO) Eprotirome (THR-Beta), Losmapimod (p38MAPK), Ezetimibe (SCH58235) (NPC1L1) Bezafibrate, Fenofibrate, Varespladib, (sPLA2), Darapladib, (LpPLA2), Lomitapide, Implitapide, Rosiglitazone, Dalcetrapib, Anacetrapib, Lorcaserin, Dapagliflozin, 35 Canagliflozin, Sergliflozin, ASP-1941, Orlistat, Exenatide, Liraglutide, Taspoglutide, Tulaglutide, Pramlintide, Lixisenatide, Albiglutide, Pioglitazone, Sodelglitazar, Netoglitazone, Indeglitazar, Naveglitazar, Lobeglitazone, Aleglitazar, Bromocriptine, Tesofensine WO 2012/064268 PCT/SE2011/051337 38 Monoamine, Alogliptin, Vildagliptin, Saxagliptin, Sitagliptin, Denagliptin, Gemigliptin, Linagliptin, Dutogliptin, Teneligliptin, LC-150444, Laropiprant extended release niacin, Simvastatin ezetimibe, Rosuvastatin fenofibrate, Rosuvastatin ezetimibe and Atorvastatin ezetimibe. 5 Combination therapy with Tredaptive@ (laropiprant), Vytorin@ (Ezetimibe/simvastatin) and Certriad (rosuvastatin calcium and fenofibric acid) may be used. According to one embodiment an IBAT inhibitor may be combined with one or more of the 10 above mentioned compounds. According to one embodiment an IBAT inhibitor of the present invention may be combined with at least one other active substance selected from dipeptidyl peptidase-IV-inhibitors, PPAR y agonists, statins and bile acid binders. 15 According to one embodiment an IBAT inhibitor as used in accordance with the present invention may be combined with at least one DPPIV, at least one PPAR y agonist, such as Sitagliptin and Pioglitazon. 20 According to one embodiment an IBAT inhibitor as used in accordance with the present invention is combined with at least one DPPIV and at least one statin e.g. Sitagliptin and Simvastatin According to one embodiment the at least one other substance may be chosen from 25 dipeptidyl peptidase-IV inhibitors, e.g. biguanides such as sitagliptin and; an incretin mimetic, a thiazolidinone, GLP-1 or an analogue thereof, and a TGR5 agonist. The at least one other substance with BAT inhibitory effect may be chosen from metformin and non-absorbable sodium dependent bile transport inhibitors e.g. 1-[4-[4-[(4R,5R)-3,3-dibutyl-7 (dimethylamino)-2,3,4,5-tetrahydro-4-hydroxy-1,1-dioxido-1-benzothiepin-5 30 yl]phenoxy]butyl]4-aza-1-azoniabicyclo[2.2.2]octane methane sulfonate. A composition of the invention may further comprise statins, such as an HMG Co-A reductase inhibitor, or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof, in association with a pharmaceutically acceptable diluent or carrier. 35 According to one embodiment the invention relates to a composition comprising one or more IBAT inhibitors of the invention and cholestyramin and/or colesevelam and/or cholestipol.

WO 2012/064268 PCT/SE2011/051337 39 According to one embodiment the invention relates to a composition comprising one or more of the compounds of Example 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 and 14 and cholestyramin and/or colesevelam and/or cholestipol. 5 According to another embodiment the invention relates to a composition comprising one or more of the compounds of 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-1'-phenyl-1' [N'-(carboxymethyl)-carbamoyl]methyl}carbamoylmethoxy)-2,3,4,5-tetrahydro-1,5 benzothiazepine (Example 5) and cholestyramin and/or colesevelam and/or cholestipol. 10 According to another embodiment the invention relates to a composition comprising one or more of the compounds of 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1 carboxy-2-m ethyl propyl)carbamoyl]-4-hyd roxybenzyl}carbamoylmethoxy)-2,3,4,5-tetrahyd ro 1,2,5-benzothiadiazepine (Example 13), and cholestyramin and/or colesevelam and/or 15 cholestipol. According to another embodiment the invention relates to a composition comprising one or more of the compounds of 1,1-Dioxo-3, 3-dibutyl-5-phenyl-7-methylthio-8-(N-{ (R)-1'-phenyl 1'-[N'-(carboxymethyl) carbamoyl] methyl} carbamoylmethoxy)-2,3,4,5-tetrahydro-1,5 20 benzothiazepine (Example 14) and cholestyramin and/or colesevelam and/or cholestipol. Use of a substance or combination of the invention reduces or inhibits recycling of bile acid salts in the gastrointestinal tract. The bile transport inhibitor may be non-systemic and systemic compounds. They may enhance L-cell secretion of enteroendocrine peptides. In 25 certain instances, increased L-cell secretion of enteroendocrine peptides is associated with induction of satiety and/or reduction of food intake and subsequent weight loss. In some embodiments, increased L-cell secretion of enteroendocrine peptides is associated with a reduction in blood and/or plasma glucose levels in a hyperglycaemic individual. In some instances, increased L-cell secretion of enteroendocrine peptides is associated with 30 increased insulin sensitivity. The invention regards methods for treating obesity or diabetes, comprising contacting the distal ileum of an individual in need of such treatment, with an IBAT inhibitor as disclosed herein. 35 In embodiments of the invention, contacting the distal ileum of an individual in with an IBAT inhibitor may reduce food intake, induce satiety, reduce blood and/or plasma glucose levels, WO 2012/064268 PCT/SE2011/051337 40 treat a metabolic disorder, reduce the weight, stimulate L-cells in the distal gastrointestinal tract, increase the concentration of bile acids and salts thereof in the vicinity of L-cells in the distal gastrointestinal tract and/or enhance enteroendocrine peptide secretion of an individual. 5 In an embodiment of the invention, an IBAT inhibitor as disclosed herein is co-administered with a second agent selected from a DPP-IV inhibitor, a biguanide, an incretin mimetic, a thiazolidinedione, a GLP-1 or an analogue thereof, and a TGR5 agonist. 10 The individual for therapy as disclosed herein may be an obese or overweight individual, a diabetic individual or a non-diabetic individual. Compounds and compositions as disclosed herein may be used for the treatment of obesity and/or diabetes, whereby a therapeutically effective amount of a combination of an IBAT 15 inhibitor and a DPP-IV inhibitor is administrated to an individual in need of such treatment. For the treatment of obesity and/or diabetes a therapeutically effective amount of a combination of an IBAT inhibitor and a TGR5 agonist may be administrated to an individual in need of such treatment. One embodiment of the invention,, is a method for the treatment of obesity and/or diabetes, whereby a therapeutically effective amount of a combination of an 20 IBAT inhibitor and at least one other active substance such as a thiazolidinedione, is administered to an individual in need of such treatment. Methods for the treatment of obesity and/or diabetes comprising the administration of a therapeutically effective amount of a combination of an IBAT inhibitor and an incretin mimic to an individual in need of such treatment are also envisaged. Obesity and/or diabetes may be treated according to the 25 invention by the administration of a therapeutically effective amount of a combination of an IBAT inhibitor and a GLP-1 or an analogue thereof, to an individual in need of such treatment. Obesity and/or diabetes may for example be treated by the administration of a therapeutically effective amount of a combination of an IBAT inhibitor and a biguanide to an individual in need of such treatment. 30 The invention further regards methods for reducing food intake in an individual in need thereof, comprising the administration of an IBAT inhibitor which is delivered or released non systemically in the distal ileum of the individual. 35 Reduction of food and caloric or induction of satiety in an individual may be performed with methods of the invention. Thus metabolic disorders may be treated and the weight may be reduced in an individual. In some embodiments, methods described herein stimulate L-cells WO 2012/064268 PCT/SE2011/051337 41 in the distal gastrointestinal tract of an individual. In some embodiments, the methods increase the concentration of bile acid and salts thereof in the vicinity of L-cells in the distal gastrointestinal tract of an individual. 5 Circulating blood or plasma glucose levels in an individual may be reduced by the administration of an IBAT inhibitor to an individual, wherein the IBAT inhibitor is delivered or released non-systemically in the distal ileum of the individual. Also, insulin secretion may be increased in an individual comprising the administration of an 10 IBAT inhibitor to an individual, wherein the IBAT inhibitor is delivered or released non systemically in the distal ileum of the individual. In embodiments of the invention wherein the enteroendocrine peptide is e.g. a GLP-1, GLP 2, PYY, oxyntomodulin, or a combination thereof, the methods described herein enhance 15 enteroendocrine peptide secretion in an individual. The distal ileum of an individual may be brought into contact with an IBAT inhibitor, and the level of a GLP-1 in the blood and/or plasma of the individual may increase by from about 2 times to about 7 times the level of GLP-1 in the blood and/or plasma of the individual prior to contacting the distal ileum of the individual with an IBAT inhibitor. 20 In embodiments of the invention, contacting the distal ileum of an individual with an IBAT inhibitor reduces the level of glucose in the blood and/or plasma of the individual by at least 20%, at least 30% or at least 40% compared to the level of glucose in the blood and/or plasma of the individual prior to contacting the distal ileum of the individual with the IBAT 25 inhibitor. Such a reduction may be kept for at least 12 or at least 24 hours compared to blood and/or plasma glucose levels in the individual prior to contacting the distal ileum of the individual with the IBAT inhibitor. The IBAT inhibitor used as described herein may be administered orally e.g. as an ileal 30 release formulation that delivers the IBAT inhibitor to the distal ileum, colon and/or rectum of an individual. In some embodiments, the IBAT inhibitor is administered as an enterically coated formulation. In some embodiments as described above, the IBAT inhibitor is a compound of Formula I or 35 Formula || as described herein.

WO 2012/064268 PCT/SE2011/051337 42 Compounds and compositions as used in accordance with the invention may be administered less than about 30 minutes or less than about 60 minutes before ingestion of food. They may also be given after ingestion of food. 5 The invention relates to methods for the prevention and/or treatment of inflammatory bowel disease, impaired bowel integrity, short bowel syndrome, gastritis, peptic ulcer, or irritable bowel disease, congestive heart failure, ventricular dysfunction, toxic hypervolemia and/or polycystic ovary syndrome, comprising contacting the distal ileum of an individual in need thereof with an IBAT inhibitor. In some embodiments, the methods further comprise the 10 administration of a DPP-IV inhibitor, a TGR5 agonist, a biguanide, an incretin mimetic, or a GLP-1 or an analogue thereof. Provided herein are methods for the prevention and/or treatment of radiation enteritis comprising contacting the distal ileum of an individual thereof with an IBAT inhibitor. In some embodiments, the methods further comprise the administration of a DPP-IV inhibitor, a TGR5 agonist, a biguanide, an incretin mimetic, or a 15 GLP-1 or an analogue thereof. Compounds and compositions as described herein may be used for reducing caloric intake in an individual, , wherein an IBAT inhibitor as described herein is delivered or released non systemically in the distal ileum of the individual. Thus, compositions for reducing circulating 20 blood and/or plasma glucose levels in an individual may comprise an IBAT inhibitor, and a pharmaceutically acceptable carrier, wherein the IBAT inhibitor is delivered non-systemically in the distal ileum of the individual. Provided herein are compositions for increasing insulin secretion, which comprise an IBAT inhibitor, and a pharmaceutically acceptable carrier, wherein the IBAT inhibitor is delivered or released non-systemically in the distal ileum of the 25 individual. In any of the aforementioned embodiments, the compositions may further comprise a DPP-IV inhibitor, a TGR5 agonist, a biguanide, an incretin mimetic, or GLP-1 or an analogue thereof. In some embodiments the IBAT inhibitors and the compositions comprising them are used 30 for reducing food intake (caloric intake) or for reducing circulating blood or plasma glucose levels, wherein the IBAT inhibitor is not absorbed systemically following oral administration. In some of such embodiments, the IBAT inhibitor is prevented from being absorbed in the stomach by its presence in a formulation that releases it in the ileum. In some of such embodiments, the IBAT inhibitor is administered in combination with a second therapeutic 35 agent selected from a DPP-IV inhibitor, a biguanide, a thiazolidinedione, an incretin mimetic, a GLP-1 or an analogue thereof, or a TGR5 agonist.

WO 2012/064268 PCT/SE2011/051337 43 According to one embodiment the invention relates to a combined oral pharmaceutical formulation comprising an IBAT inhibitor compound or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof and/or a bile acid binder or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof, 5 wherein the formulation is designed to deliver the bile acid binder in the colon and the IBAT inhibitor in the small intestine and wherein the combined formulation is intended for administration of the IBAT inhibitor and the bile acid binder simultaneously, separately or sequentially. 10 In one embodiment the acid binder is formulated in a separate formulation with the IBAT inhibitor formulation releasing the drug immediately or delayed in the distal jejunum or the proximal ileum and the bile acid binder formulation releasing the drug in the colon. In still an embodiment, the formulation comprises a core comprising the bile acid binder 15 formulated for release in the colon surrounded by an outer layer comprising an IBAT inhibitor and formulated for immediate release or for delayed release in the distal jejunum or the proximal ileum. Medicinal and pharmaceutical use of the invention 20 According to another feature of the invention there is provided an oral pharmaceutical formulation comprising an IBAT inhibitor compound or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof and a bile acid binder, wherein the formulation is designed to deliver the bile acid binder in the colon for use in the production of 25 an IBAT inhibitory effect in a warm-blooded animal, such as man. According to another feature of the invention there is provided an oral pharmaceutical formulation comprising an IBAT inhibitor compound or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof and a bile acid binder, and at least one of 30 the above mentioned other active compounds and a bile acid binder as disclosed herein, wherein the formulation is designed to deliver the bile acid binder in the colon for use in prophylaxis or treatment of any of the herein mentioned medical indications in a warm blooded animal, such as man. 35 According to another feature of the invention there is provided an oral pharmaceutical formulation comprising an IBAT inhibitor compound or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof and a bile acid binder, wherein the WO 2012/064268 PCT/SE2011/051337 44 formulation is designed to deliver the bile acid binder in the colon for use in prophylaxis or treatment of any of the herein mentioned medical indications in a warm-blooded animal, such as man. 5 According to another feature of the invention there is provided an oral pharmaceutical formulation comprising an IBAT inhibitor compound or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof and a bile acid binder, and at least one of the above mentioned other active compounds and a bile acid binder of the invention wherein the formulation is designed to deliver the bile acid binder in the colon for use in prophylaxis 10 or treatment of any of the herein mentioned medical indications in a warm-blooded animal, such as man. According to another feature of the invention there is provided an oral pharmaceutical formulation comprising an IBAT inhibitor compound or a pharmaceutically acceptable salt, 15 solvate, solvate of such a salt or a prodrug thereof and a bile acid binder, wherein the formulation is designed to deliver the bile acid binder in the colon for use in the preparation of a pharmaceutical for use in prophylaxis or treatment of any of the herein mentioned medical indications in a warm-blooded animal, such as man. 20 According to another feature of the invention there is provided an oral pharmaceutical formulation comprising an IBAT inhibitor compound or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof and a bile acid binder, and at least one of the above mentioned other active compounds and a bile acid binder as disclosed herein, wherein the formulation is designed to deliver the bile acid binder in the colon, for use in the 25 preparation of a pharmaceutical for use in the prophylaxis or treatment of any of the herein mentioned medical indications in a warm-blooded animal, such as man. In an embodiment of the invention, there is provided a method of treating any of the herein mentioned medical conditions in a warm-blooded animal, such as man, whereby an effective 30 amount of an IBAT inhibitor compound or a pharmaceutically acceptable salt, solvate, solvate of such a salt or a prodrug thereof, is administered simultaneously, sequentially or separately in combination with an effective amount of a bile acid binder to a subject in need of such treatment. . 35 In an embodiment of the invention, there is provided a method of treating any of the herein mentioned medical conditions in a warm-blooded animal, such as man, whereby an effective amount of an IBAT inhibitor compound or a pharmaceutically acceptable salt, solvate, WO 2012/064268 PCT/SE2011/051337 45 solvate of such a salt or a prodrug thereof, and at least one of the above mentioned other active compounds, is administered simultaneously, sequentially or separately in combination with an effective amount of a bile acid binder to a subject in need of such treatment. 5 Dosage forms Pharmaceutical compositions may be formulated as a dosage form. A dosage form may comprise a compound of formula I or a compound of formula 1l, suitable for administration to an individual. Suitable dosage forms include, by way of non-limiting example, aqueous oral dispersions, liquids, gels, syrups, elixirs, slurries, suspensions, solid oral dosage forms, 10 aerosols, controlled release formulations, fast melt formulations, effervescent formulations, lyophilized formulations, tablets, powders, pills, dragees, capsules, delayed release formulations, extended release formulations, pulsatile release formulations, multiparticulate formulations, and mixed immediate release and controlled release formulations. 15 A pharmaceutical solid dosage form may optionally include an additional therapeutic compound as described herein and one or more pharmaceutically acceptable additives such as a compatible carrier, binder, filling agent, suspending agent, flavouring agent, sweetening agent, disintegrating agent, dispersing agent, surfactant, lubricant, colorant, diluent, solubilizer, moistening agent, plasticizer, stabilizer, penetration enhancer, wetting agent, anti 20 foaming agent, antioxidant, preservative, or one or more combination thereof. In some aspects, using standard coating procedures, such as those described in Remington's Pharmaceutical Sciences, 20th Edition (2000), a film coating is provided around the formulation of the compound of Formula I or formula 1l. In one embodiment, a compound as described herein is in the form of a particle and some or all of the particles of the compound 25 are coated. In certain embodiments, some or all of the particles of a compound as described herein are microencapsulated. In some embodiments, the particles of the compound described herein are not microencapsulated and are uncoated. Pharmaceutical compositions may be formulated as known in the art using one or more 30 physiologically acceptable carriers including, e.g., excipients and auxiliaries which facilitate processing of the active compounds into preparations which are suitable for pharmaceutical use. In certain embodiments, proper formulation is dependent upon the route of administration chosen. A summary of pharmaceutical compositions and carriers may be found, for example, in Remington: The Science and Practice of Pharmacy, Nineteenth Ed 35 (Easton, Pa.: Mack Publishing Company, 1995); Hoover, John E., Remington's Pharmaceutical Sciences, Mack Publishing Co., Easton, Pa. 1975; Liberman, H. A. and Lachman, L., Eds., Pharmaceutical Dosage Forms, Marcel Decker, New York, N.Y., 1980; WO 2012/064268 PCT/SE2011/051337 46 and Pharmaceutical Dosage Forms and Drug Delivery Systems, Seventh Ed. (Lippincott Williams & Wilkins 1999). The pharmaceutical formulations are administered to an individual in any manner, including 5 one or more of multiple administration routes, such as, by way of non-limiting example, oral, parenteral (e.g., intravenous, subcutaneous, intramuscular), intranasal, buccal, topical, rectal, or transdermal administration routes. An IBAT inhibitor of Formula I or formula II is used in the preparation of medicaments for the 10 prophylactic and/or therapeutic treatment of obesity and/or diabetes. A method for treating any of the diseases or conditions described herein in an individual in need of such treatment, involves the administration of pharmaceutical compositions containing at least one IBAT inhibitor described herein, or a pharmaceutically acceptable salt, pharmaceutically acceptable N-oxide, pharmaceutically active metabolite, pharmaceutically acceptable 15 prodrug, or pharmaceutically acceptable solvate thereof, in therapeutically effective amounts to said individual. A dosage form allowing for controlled release of an active agent in the distal jejunum, proximal ileum, distal ileum and/or the colon is also within the scope of the invention. In some 20 embodiments, a dosage form comprises a polymer that is pH sensitive e.g., a MMX@ matrix from Cosmo Pharmaceuticals and allows for controlled release of an active agent in the ileum and/or the colon. Examples of such pH sensitive polymers suitable for controlled release include and are not limited to polyacrylic polymers (e.g., anionic polymers of methacrylic acid and/or methacrylic acid esters, e.g., Carbopol. @ Polymers Cas number 25 9063-87-0; 600-07-7) that comprise acidic groups (e.g. -COOH, -S0 3 H) and swell in basic pH of the intestine (e.g., pH of about 7 to about 8). In some embodiments, a dosage form suitable for controlled release in the distal ileum comprises microparticulate active agent (e.g. micronized active agent). In some embodiments, a non-enzymatically degrading poly(dl lactide-co-glycolide) (PLGA) core is suitable for delivery of an IBAT to the distal ileum. In 30 some embodiments, a dosage form comprising an IBAT is coated with an enteric polymer (e.g., Eudragit.@ S-100, Cas number 25086-15-1), cellulose acetate phthalate, polyvinylacetate phthalate, hydroxypropylmethylcellulose phthalate, anionic polymers of methacrylic acid, methacrylic acid esters or the like) for site specific delivery to the ileum and/or the colon. In some embodiments, bacterially activated systems are suitable for 35 targeted delivery to the ileum. Examples of micro-flora activated systems include dosage forms comprising pectin, galactomannan, and/or Azo hydrogels and/or glycoside conjugates (e.g., conjugates of D-galactoside, beta-D-xylopyranoside or the like) of the active agent.

WO 2012/064268 PCT/SE2011/051337 47 Examples of gastrointestinal micro-flora enzymes include bacterial glycosidases such as, for example, D-galactosidase, beta-D-glucosidase, alpha-L-arabinofuranosidase, beta-D xylopyranosidase or the like. 5 Coated units may be filled into hard gelatine capsules or mixed with tablet excipients, such as fillers, binders, disintegrants, lubricants and other pharmaceutically acceptable additives, and be compressed into tablets. The compressed tablet is optionally covered with film forming agents to obtain a smooth surface of the tablet and further enhance the mechanical stability of the tablet during packaging and transport. Such a tablet coat, which may be 10 applied on a multiple unit tablet or a conventional tablet, may further comprise additives like anti-tacking agents, colorants and pigments or other additives to improve the tablet appearance. A combination therapy according to the invention should preferably comprise simultaneous, 15 separate or sequential administration of an IBAT inhibitor compound and a bile acid binder. The IBAT inhibitor could preferably be formulated for ileum delivery and the bile acid binder could preferably be formulation for colon release. Dosage 20 A suitable unit dose of an IBAT inhibitor compound of formula I or formula II will vary with respect to the patient's body weight, condition and disease severity. The dose will also depend on if it is to be used for prophylaxis or for therapy, as well as the route of administration. The daily dose can be administered as a single dose or divided into one, two, three or more unit doses. An orally administered daily dose of an IBAT inhibitor is preferably 25 within 0.1 - 1000 mg, more preferably 1 - 100 mg. A pharmaceutical formulation according to the present invention with a targeted delivery in the gastro intestinal tract provides a reduced systemic exposure, as can be measured by the area under the drug plasma concentration versus time curve (AUC) or 7a-hydroxy-4 30 cholesten-3-one (C4), while maintaining or even increasing the therapeutic effect, as e.g. measured by serum cholesterol reduction. A combination comprising an IBAT inhibitor and a bile acid binder may comprise a low daily dose of the bile acid binder, such as up to 5 g of a resin, more preferably up to 2 g. A dosage 35 form with colon release of the bile acid binder may be constructed by any of the above described principles for delayed release formulations.

WO 2012/064268 PCT/SE2011/051337 48 A combination comprising an IBAT inhibitor and a bile acid binder may comprise a low daily dose of the bile acid binder, such as up to 5 g of a resin, more preferably up to 4 g such as up to 3 g, or up to 2 g or up to 1 g. A suitable daily dose of the bile acid binder may be 0.1-5 g, 0.5- 4 g, 1-3 g, 2-4 g, 2-3 g.. A dosage form with colon release of the bile acid binder may 5 be constructed by any of the above described principles for delayed release formulations. A tablet may consist of an inner core of 1-1000 mg, e.g. 200-800 mg, 50-400 mg, 10-200 mg or 20-80 mg acid binder in a colonic delivery formulation and an outer lamina with 1-100 mg, 5-50 mg e.g. 1-20 mg of an IBAT inhibitor as described herein. 10 The daily dose of an IBAT inhibitor and /or bile acid binder as described herein can be administered as a single dose or divided into one, two, three or more unit doses. The BAT inhibitor may be administrated once a day and the acid inhibitor one, two or three 15 times a day. In one embodiment the IBAT inhibitor and the bile acid binder are administrated in combination one, two or three times a day. In one embodiment of the invention, the bile acid binder is colesevelam. 20 The daily recommended total dose of colesevelam to block bile acid absorption in total gut of humans may be up to 3750 mg/day. The invention also regards a method for the treatment and/or prophylaxis of obesity or diabetes, in a warm-blooded animal, such as man, whereby an effective amount of a 25 compound, a composition or a combination as disclosed herein, is administered to a subject in need of such treatment and/or prophylaxis. A method for treating any of the diseases or conditions described herein in an individual may involve the administration of a pharmaceutical composition containing at least one IBAT 30 inhibitor as described herein, or a pharmaceutically acceptable salt, pharmaceutically acceptable N-oxide, pharmaceutically active metabolite, pharmaceutically acceptable prodrug, or pharmaceutically acceptable solvate thereof, in therapeutically effective amounts to said individual. 35 Further, the invention relates to a kit comprising compound or a composition according to the invention and optionally also an instruction for use.

WO 2012/064268 PCT/SE2011/051337 49 Examples of compounds useful in accordance with the invention All references cited herein are hereby incorporated by reference in their entirety. 5 The expression "comprising" as used herein should be understood to include, but not be limited to, the stated items. Example 1 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-(carboxymethyl)carbamoyl] 10 benzyl} carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5-benzothiadiazepine, Mw. 696,89. This compound is prepared as described in Example 2 of W03022286. Example 2 1,1-Dioxo-3, 3-dibutyl-5-phenyl-7-methylthio-8- (N-{(R)-a-[N'-((S)-1-carboxyethyl) carbamoyl] 15 benzyl} carbamoylmethoxy)-2, 3,4, 5-tetrahydro-1, 5-benzothiazepine, Mw. 709,92. This compound is prepared as described in Example 2 of W003106482. Example 3 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1-carboxypropyl) 20 carbamoyl]benzyl}carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5-benzothiadiazepine, Mw. 724,94. This compound is prepared as described in Example 6 of W03022286. Example 4 25 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((R)-1-carboxy-2 m ethylthioethyl)carbamoyl]benzyl}carbamoylmethoxy)-2,3,4,5-tetrahyd ro- 1,2,5 benzothiadiazepine, Mw. 757,01. This compound is prepared as described in Example 7 of W03022286. 30 Example 5 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1-carboxypropyl) carbamoyl] 4-hydroxybenzyl}carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5-benzothiadiazepine, Mw. 740,94. This compound is prepared as described in Example 29 of W03022286. 35 WO 2012/064268 PCT/SE2011/051337 50 Example 6 1,1 -Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((R)-1 -carboxy-2-methylthio ethyl)carbamoyl]-4-hyd roxybenzyl}carbamoylmethoxy)-2,3,4,5-tetrahyd ro- 1,2,5 benzothiadiazepine, Mw. 773,00. 5 This compound is prepared as described in Example 30 of W03022286. Example 7 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1-carboxy-2 m ethylpropyl)carbamoyl]benzyl}carbamoyl methoxy)-2,3,4,5-tetrahyd ro- 1,2,5 10 benzothiadiazepine, Mw. 738,97. This compound is prepared as described in Example 15 of W03022286. Example 8 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1-carboxy-2-(R) 15 hydroxypropyl)carbamoyl]-4-hydroxybenzyl}carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5 benzothiadiazepine, Mw. 756,94. This compound is prepared as described in Example 26 of W03022286. Example 9 20 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1-carboxybutyl) carbamoyl] 4-hydroxybenzyl}carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5-benzothiadiazepine, Mw. 754,97. This compound is prepared as described in Example 28 of W03022286. 25 Example 10 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1-carboxyethyl) carbamoyl]benzyl}carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5-benzothiadiazepine, Mw. 710,91. This compound is prepared as described in Example 5 of W03022286. 30 Example 11 1,1-Dioxo-3, 3-dibutyl-5-phenyl-7-methylthio-8- (N-{(R)-u-[N'-((S)-1-carboxypropyl) carbamoyl]-4-hydroxybenzyl} carbamoylmethoxy) -2, 3,4, 5-tetrahydro-1, 5-benzothiazepine, Mw. 739,95. 35 This compound is prepared as described in Example 1 of W03022286.

WO 2012/064268 PCT/SE2011/051337 51 Example 12 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1-carboxyethyl)carbamoyl]-4 hydroxybenzyl}carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5-benzothiadiazepine, Mw. 726,91. This compound is prepared as described in Example 11 of W03022286. 5 Example 13 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-a-[N-((S)-1-carboxy-2 m ethylpropyl)carbamoyl]-4-hyd roxybenzyl}carbamoylmethoxy)-2,3,4,5-tetrahyd ro- 1,2,5 benzothiadiazepine, Mw. 754,97. 10 This compound is prepared as described in Example 27 of W03022286. Example 14 1,1-Dioxo-3, 3-dibutyl-5-phenyl-7-methylthio-8-(N-{ (R)- 1'-phenyl- 1'-[N'-(carboxymethyl) carbamoyl] methyl} carbamoylmethoxy)-2, 3,4, 5-tetrahydro-1, 5-benzothiazepine, Mw. 15 695,90. This compound is prepared as described in Example 43 of W00250051. Example 15 Pharmaceutical effect Mean Inhibitory effect (%) 20 ISBT Hu HEK Uptake SPA 13203 IBAT HUM hleal Bile Acid Transporter Human HEK Glycocholic acid Uptake Radiometric - SPA Inhibitor IC50 Mean IC50 (nM) was determined for the compounds of examples 1-14 Test system 25 Animals: Species Mouse; Strain ApoE knock out; Sub strain C57BL/6; Sex Female; Total No. of animals 70; Body weight range 20 g to 22 g; Supplier M6legaard's Breeding (Skensved, Denmark); Identification method ID cards (bar code). 30 Acclimatisation: At least one week at the Section of Laboratory; Animal Resource at AstraZeneca; Housing conditions: Kept five by five in cages (Makrolon 111, 7 dm2) in a room with regulated temperature (22'C), relative humidity (40% to 60%) and a 12/12 hours light/dark cycle. Diet: Free access to R3 pellets (Lactamin, Vadstena, Sweden) during the housing and experimental period. Water: Free access to tap water during the housing and 35 experimental period.

WO 2012/064268 PCT/SE2011/051337 52 Experimental procedures The animals were orally administered vehicle (n=3 )or the compound of Example 14 (0.156 (n=3) , 0.625 (n=3) or 2.5 pmol/kg (n=3)) at 13:00 o'clock on the experimental day. Thirty 5 minutes later, a trace amount of 7 5 SeHCAT ( 75 Se-homo-tauro-cholic acid) (0.1 mCi per 0.1 mL per mouse) was orally given to each mouse. Twenty-four hours after 75 SeHCAT administration, the animals were killed by C02 inhalation. At sacrifice, the gall bladder and the whole intestine were removed, and the faeces during the 24-hour period after 75 SeHCAT administration was collected for each mouse. The gamma radioactivities of 10 7 5 SeHCAT in the faeces and in the gall bladder-intestine were separately counted by 1282 CompuGamma CS Gamma counter (Wallac oy, Turku, Finland). The stability as well as the quantity of the 7 5 SeHCAT administered to each mouse, were controlled with an additional 75 SeHCAT aliquot following the same experimental process as other tested samples in the study. 15 Data analysis The sum of the gamma counts from both the faeces and the gall bladder-intestine was considered as the total recovered 75 SeHCAT, which was averaged around 85% of the total 75 SeHCAT administered to each mouse. Of the recovered radioactivity of 75 SeHCAT, the 20 percentage of the 75 SeHCAT detected in the faeces was considered as the faecal excretion while that in the gall bladder-intestine as body retention. Inhibitory effect of the compound of Example 14 on 7 5 SeHCAT intestinal absorption was calculated following the 75 SeHCAT body retention and the faecal excretion, and the ED50 of the compound was estimated following the dose-effect curve. 25 Results The mean IBAT inhibitory effect (%) at a dose (pmol/kg): 0.156 was determined for the compounds of examples 1-14 and is reported in Table 1. 30 WO 2012/064268 PCT/SE2011/051337 53 Table 1 % inhibition Mean IC50 Example Structure 0.156 mol/kg nM 0 0 HO NH NN HO N O 9 . 0 43 0,45 S N SH 0 H NN HO N 00 2. 0 , H S 55 0,39 s N 5HO N N H NN HO N 7. HO0 S 3. o a Ho 63 0,18 S N H HO N HO N oG9 4. H a 63 0,35 S N O H O N 0 5. HO H 00 74 0,16 S N OH 0 0 N HO N 6. 0 H 0 59 S N 0 ~ HY - 0 0 S H 7. HO' 01 N 0 N 66 0,36 OH 0 < 8. HO 1N 046 0,11 HO 0 H Sa

OH

WO 2012/064268 PCT/SE2011/051337 54 H O H 10. HON o O 68 0,2 S H 13. Ho O _ N 66 0,12 HO O H o O o 11. HO H 0 63 0,15 HO N 12. -f0H63 0,3 C31 13 N 0 68 0,13 0 S 0 0 H HO _ N 14. HO 0 H 0 S1 28 1,2 S N Example 16 Interruption of bile acid circulation by inhibition of the bile acid transporter Sic 10a2 improves 5 triglyceride metabolism and normalizes plasma glucose levels.

WO 2012/064268 PCT/SE2011/051337 55 Experimental procedures Animals S/c10a2+/- and S/c10a2-/- mice were generated at AstraZeneca R&D, M6|ndal, as described 5 below and as outlined by Dawson, Haywood, Craddock, Wilson, Tietjen, Kluckman, Maeda Parks. J Biol Chem 2003;278:33920-7.. Targeting the S/c10a2 locus The targeting vector used to modify the mouse Sic10a2 locus was a kind gift from P.Dawson 10 and has been previously described Dawson et. Al 2003. In brief, it consisted of a -14kb 5'homology arm, an inverted Neo (neomycin phosphotransferase) cassette driven off the PGK (phosphoglycerate kinase) promoter and a 1.6kb 3' homology arm. The targeting vector was designed so that correct targeting would result in most of intron 2, exon 3, intron 3 and the very 5' end of exon 4 being deleted and replaced by the Neo cassette in order to 15 inactivate the S/c10a2 gene (see Fig. 1A). B, BamH/; H, Haell/. After linearization, the targeting construct was electroporated into R1 ES cells (derived from 129/SvJ) and neomycin-resistant clones were selected in G-418-containing (300 pg/ml) media. Of 400 G418-resistant clones screened, 2 targeted clones were identified using a PCR screening over the short arm 20 and then confirmed by Southern analysis. The primers used for detecting the targeted allele were a forward primer located in the inverted Neo cassette and a reverse primer located downstream of the short arm (5'-cgtactggggcatagaatctttgc-3'). The same reverse primer was combined with a forward primer in intron 3 (5'-ctcttcctatgaagctaaaggggc 3') for detection of the wild-type allele. One positive clone was expanded and injected into 25 C57B11/6 blastocysts to generate chimeric mice. Chimeric males were backcrossed to C57B11/6 females and genotyping of the offspring was performed from tail biopsies by both PCR and Southern to confirm germline transmission. To verify that the targeted SLC10A2 allele resulted in a null mutation, total RNA was prepared from the kidneys and intestines of 30 8-10 week old homozygous, heterozygous, and wild-type littermates using TRIzol Reagent according to the manufacturer's instructions (Invitrogen, Paisley, UK). cDNA was synthesized using Super-script || Rnase H- Reverse Transcriptase and random hexamer primers (Invitrogen, Paisley, UK). TaqMan real-time PCR was performed using the ABI PRISM 7700 Sequence Detector System (Applied Biosystems, Warrington, UK). All samples 35 were run in triplicate and data were normalized using the mouse acidic ribosomal phosphoprotein PO (M36B4) as an internal control. The TaqMan primers and probe for WO 2012/064268 PCT/SE2011/051337 56 SL C10A2 were: 5'-accacttgctccacactgctt-3'(forward), 5'-acccacatcttggtgtagacga-3'(reverse) and 5'-ccttggaatgatgcctctttgcctc-3' (probe). A diet enriched in sucrose (D12329, Research Diets, NJ) together with drinking water 5 supplemented with 10% fructose was used for the high carbohydrate experiment. Animals had free access to the diet for two weeks. Control animals received standard mouse chow and tap water. Male ob/ob animals were from Taconic, DK. Ob/ob animals were gavaged with a specific Slc10a2 inhibitor, the compound of Example 14 or a control vehicle for 11 days. Animals had free access to food and water. All animal care and experiments were 10 conducted in accordance with accepted standards of humane animal care and approved by the Ethics Committee of G6teborg University. Plasma analysis Blood was centrifuged, and plasma was analyzed for total cholesterol and triglycerides (TGs) 15 using the IL TestTM cholesterol 181618-10 and TG 181610-60 kits on the Monarch 2000 system (IL Scandinavia, Gothenburg, Sweden). Lipoprotein cholesterol profiles were obtained by separation of 10 pl of plasma using a micro fast protein liquid chromatography (FPLC) system for the generation of lipoprotein profiles (15). Plasma insulin levels were analyzed using a rodent insulin RIA kit (Linco, St. Charles, MI). Total plasma glucose was 20 analyzed using the IL Test (IL Scandinavia, Gothenburg, Sweden) on the Monark 2000 system. Blood glucose was determined using a Bayer Elite glucometer (Bayer diagnostics, Germany). Plasma free fatty acids were analyzed employing a commercial 3 NEFA kit (Wako Chemicals USA Inc.Richmond, Virginia). Serum levels of 7a-hydroxy-4-cholesten-3-one (C4) were used as an indirect measurement of CYP7A1 activity, and analyzed in either pooled or 25 individual plasma samples by high pressure liquid chromatography . Enzymatic activities HMGCoA reductase and CYP7A1 enzymatic activities were assayed in hepatic microsomes as described. 30 RNA extraction and quantitative real time PCR Total RNA was extracted from frozen livers or distal ileum with TRIzol reagent (Invitrogen, Carlsbad, CA). The RNA was DNase-treated with RQ1 Dnase (Promega, Madison, WI). cDNA synthesis and quantitative real time PCR was performed employing HPRT as 35 endogenous control (18). Primer and probe information are available upon request.

WO 2012/064268 PCT/SE2011/051337 57 Liver protein and immunoblottinq A ligand blot employing 12 5 1-labeled rabbit P-VLDL was used to detect LDL receptors in liver as described Rudling, Norstedt, Olivercrona, Reihner, Gustavsson, Angelin. Proc. NatI. Acad. Sci. USA 1992;89:6983-7. Hepatic SR-BI was assayed by immunoblot using a rodent 5 specific rabbit polyclonal antibody (Novus Biologicals Inc., Littleton, CO), as described Galman, Angelin, Rudling. Endocrinology 2002;143:1809-16.. Cyp7al protein levels were assayed in liver microsomal protein samples by immunoblot using a rabbit polyclonal antibody directed against the C-terminus of the CYP7A1 Lundasen, Liao, Angelin, Rudling. J. Biol. Chem. 2003;278: 43224-43228). To detect SREBP1 protein, cytoplasmic and nuclear 10 protein preparations from liver were performed using the NE-PER reagent (Pierce), including Complete protease inhibitor (Roche), 1 mM phenyl-methylsulfonyl fluoride, 0.5 mM leupeptin, 5 pg/ml Calpain inhibitor I (Biomol, PA), following the manufacturer's instructions. 50 pg and 25 pg of cytoplasmic and nuclear liver protein fractions, respectively, were electrophoresed on NuPage Bis-Tris gels (Invitrogen) and transferred to nitrocellulose membranes. 15 Membranes were blocked in 5% skimmed milk powder and incubated with a mouse monoclonal antibody raised against the N'-terminus of SREBP1 (Labvision Corporation, CA) at 1.5 pg/mL in 5% skimmed milk powder for two hours at room temperature. An HRP conjugated goat anti-mouse F(ab)2 antibody (Pierce) was used for detection of specific signals together with Supersignal reagent (Pierce) and a Fuji BAS 1800 analyzer (Fuji Photo 20 Film Co.). To analyze phosphorylated liver proteins by western blot, total liver protein homogenates were prepared from frozen tissue by homogenization using a polytron followed by sonication in a buffer containing 20 mM Tris-HCI, pH 7,4, 1% Triton X-100, 10% Glycerol, 150 mM NaCl, 2 mM EDTA, 25 mM betaglycerophosphate, 20 mM sodium fluoride, 1mM sodium orthovanadate, 2 mM sodium pyrophosphate, 1 mM benzamidine, 1 mM phenyl 25 methylsulfonyl fluoride, 0.5 mM leupeptin, Complete proteinase inhibitor (Roche), and then centrifuged at 14 000 rpm in a microcentrifuge, and the supernatant was recovered. 50 pg protein was loaded on NuPage Bis-Tris gels (Invitrogen) and transferred to nitrocellulose membranes. Membranes were blocked in Starting Block-PBS (Pierce) and incubated in 3 % BSA with phosphorylation site specific antibodies, pAkt1, pErkl/2, pMekl/2, pAmpk as 30 indicated in figures overnight at +4C, then stripped with Restore (Pierce) and reprobed with antibodies detecting total amounts of Akt1, Mekl/2, Erkl/2, and Ampk and finally stripped and reprobed with an antibody against beta-actin (Abcam) to verify gel loading. Antibodies against kinases were purchased from Cell Signalling Technology, Inc. Blots were developed as described above. 35 WO 2012/064268 PCT/SE2011/051337 58 Determination of liver TGs and cholesterol Liver cholesterol was determined as previously described and liver TGs were extracted and determined employing a commercially available kit (Roche Applied Science, Indianapolis). 5 Statistics Data show mean +/- SEM. The significance of differences between groups was tested by 1 way ANOVA followed by post-hoc comparisons according to Dunnett using GraphPad PrismSoftware. For the studies on ob/ob mice, the significance between groups was tested by Student's t-test. 10 RESULTS Generation of S/c10a2-/- mice Generation of S/c10a2-/- mice was performed as described in detail in Fig 1 experimental 15 procedures. Southern blotting, quantitative real time PCR and immunoblotting confirmed appropriate targeting and lack of S/c10a2 expression in the null mice (Fig. 1 and not shown.) Sic10a2-/- animals were viable and fertile. No abnormalities in behaviour, gross appearance or survival were seen, consistent with the previous report by Dawson et al.2003. 20 Increased BA synthesis in male S/c10a2+/-and Sic10a2-/- mice An established response following disruption of the enterohepatic circulation of Bile Acids (Bas) is an induced synthesis of BAs (2,6-8,22). Indeed, when the hepatic mRNA levels for CYP7A1, the rate-limiting enzyme in the synthesis of BAs, were assayed by quantitative real time PCR 25 (qrtPCR), there was an -7-fold induction in S/c10a2-/- mice (Fig. 2A). Also, in the Sic10a2+/- mice there was a clear but less pronounced (-3-fold) increase in CYP7A1 mRNA. Consistent with the CYP7A1 mRNA increase, the enzymatic activity and the protein mass of CYP7A1 were higher in both S/c10a2+/- and -/- mice, when examined in pooled microsomes (Figs 2B and 2C). The CYP7A1 reaction product 7a-hydroxy-4-cholesten-3-one 30 (C4), present in blood serum, has been demonstrated to be an accurate marker of CYP7A1 enzyme activity (16). Analysis of pooled serum from both heterozygous and homozygous animals revealed higher serum C4 levels as compared to control animals (Fig. 2D). In conjunction with the observed increase in CYP7A1, the mRNA levels for the 12 alphahydroxylase, CYP8B1, were also induced dose dependently (Fig. 2E). When the 35 circulation of BAs is disrupted, the amount of available ligand for the hepatic nuclear BA receptor FXR decreases. In line with this, decreased hepatic mRNA levels of the FXR target gene, Small Heterodimer Partner (SHP), a suppressor of CYP7A1 gene transcription, were WO 2012/064268 PCT/SE2011/051337 59 found in heterozygous and homozygous mice, with the most drastic change in the latter group (Fig. 2F). Lowered plasma TGs in S/c10a2+/- and S/c10a2-/- mice 5 Plasma total TGs were significantly reduced by 22% in S/c10a2+/- mice and by 35% in Sic10a2-/- mice compared to controls (Fig. 3A). The plasma cholesterol and TG lipoprotein profiles were then analyzed by fast performance liquid chromatography (FPLC) (Figs 3B and C). The plasma cholesterol profiles did not vary notably between controls and homozygous animals. However, larger changes were found in the plasma TG profiles of these mice (Fig. 10 3C). In S/c10a2+/- mice, TGs were reduced within LDL, whereas in S/c10a2-/- mice TGs were reduced in both VLDL- and LDL (Fig. 3C). Plasma glucose and insulin were however not altered in S/c10a2- /- animals in this experiment (not shown). Adaptation of hepatic cholesterol metabolism to BA deficiency 15 There were no changes in the hepatic expression of the LDL-receptor or the HDL-receptor SR-BI, neither at mRNA nor at protein levels (data not shown). The increased need for cholesterol as substrate for CYP7A1 in the livers of S/c10a2+/- and S/c10a2-/- mice was reflected in increased enzymatic activity of the hepatic HMGCoAreductase, with a 2-fold increase in the heterozygous and a 3.5-fold increase in homozygous animals, based upon 20 analysis of pooled microsomal samples (Fig. 3D). The increases in hepatic HMGCoA reductase mRNA levels had a similar pattern, although smaller differences were observed between groups (Fig. 3D). The gene expression of the hepatic sterol transporters ABCG5 and ABCG8 were suppressed by up to 50% in a gene-dose dependent manner (Fig. 3E). In line with the findings for HMGCoA reductase, the hepatic levels of SREBP2 mRNA were 25 increased in both S/c10a2+/- and S/clOa2-/-mice (Fig. 3F). Intriguingly, the regulation of the SREBP1c gene in the liver displayed an opposite pattern, with reduced mRNA levels in heterozygous and homozygous mice compared to controls, again in a gene-dose dependent manner (Fig. 3F). 30 Hepatic TG production is suppressed in SLC1OA2-/- mice To further explore TG metabolism in this animal model of BA malabsorption, S/c10a2-/- and wt control animals received a sucrose-rich diet (SRdiet), to increase substrate availability, for two weeks while animals on chow served as controls. Neither plasma glucose, insulin nor food intake were significantly different between S/c10a2-/-and respective wt control group, for 35 both diet types used (data not shown). Likewise, a densitometric x-ray analysis (DEXA) did not reveal any significant changes in body composition of S/c10a2-/- animals as compared to wt controls (not shown). The hepatic TG content tended to be lower in S/c10a2-/- mice fed WO 2012/064268 PCT/SE2011/051337 60 regular chow (Fig. 4A). This difference was more evident on the SR diet; hepatic TGs increased by 120% in wt animals, whereas in S/c10a2-/- animals this increase was significantly blunted (Fig. 4A). Also, hepatic cholesterol was lower in S/c10a2-/- mice fed the SR diet compared to wt controls on this diet (Fig.4A). Since the lower hepatic TG levels in 5 S/c10a2-/- mice could be due to decreased fatty acid synthesis the mRNA levels of ACL, ACC, FAS and SCD1 by qRT PCR were measured The gene expression of these enzymes was reduced in the livers of S/c10a2-/- animals (Fig. 4B); this finding was more pronounced when animals were challenged with the SR diet. The transcription factor SREBP1c is crucial for optimal activation of most genes in the fatty acid synthesis pathways (23). The protein 10 expression of SREBP1c (mature and precursor form) was reduced in the S/clOa2-/- mice, as evaluated by Western blot on cytoplasmic and nuclear protein fractions using an antibody against the N'-terminus of SREBP1 c (Fig. 4C). Disruption of BA circulation alters the expression of genes involved in hepatic 15 glucose handling Hepatic TG synthesis is dependent on substrate flow in the glycolytic pathway and thus on the activity and gene expression of glucose metabolizing enzymes The mRNA levels of the hepatic glycolytic enzyme glucokinase (GK) were unaltered in S/c10a2-/- animals as compared to wt controls both on chow and on the SR diet (Fig. 4D). However, the mRNA 20 levels of liver pyruvate kinase (LPK) were reduced by 30% in S/c10a2-/- on chow (Fig. 4D). Interestingly, feeding the SR diet to S/c10a2-/- mice resulted in a 4.3-fold upregulation of LPK mRNA from basal levels, whereas in wt control animals there was a more modest 1.8-fold stimulation under the same conditions. Determination of the NADPH generating enzyme glucose-6-phosphate dehydrogenase (G6PDH) mRNA showed 25 increased levels in S/c10a2-/- animals as compared to wt mice on regular chow (Fig. 4E). Feeding the SR diet resulted in a three-fold increase of G6PDH mRNA in wt mice whereas in Sic10a2-/- mice there was only a modest 60% increase (Fig. 4E). It was therefore of interest to also determine if the gene expression of malic enzyme (ME), also part of a NADPH generating step converting malate to pyruvate was changed in S/clOa2-/-livers. As seen from 30 Fig. 4E, ME mRNA tended to be decreased in S/c10a2-/- mice on regular chow and increased during the SR diet to similar extents in wt and S/clOa2-/- animals. Inhibition of Slc1Oa2 in ob/ob mice reduces plasma glucose and TGs in conjunction with increased hepatic FGF21 mRNA. 35 Although serum glucose in S/clOa2-/- mice was not changed, these animals were hypolipidemic. It was therefore evaluated if beneficial effects could be obtained in ob/ob mice, a model where plasma glucose and TGs are chronically elevated. Ob/ob mice were WO 2012/064268 PCT/SE2011/051337 61 treated with the specific Slc2al0 inhibitor or the compound of Example 14 for 11 days. Fasting levels of glucose were reduced by 30% in drug-treated ob/ob mice compared to vehicle treated animals (Fig. 5A). Likewise, this treatment reduced fasting insulin levels by 50% in drug-treated animals. Also, the total plasma TG levels were reduced by 73% in drug 5 treated controls, whereas total plasma cholesterol tended to be slightly (16%) increased (Fig. 5B). It was previously found that the mRNA levels of the hormone FGF21 are increased 10 fold in ob/ob livers compared to that of wt animals Lundasen, Hunt, Nilsson, Sanyal, Angelin, Alexson, Rudling.2007 Biochem. Biophys. Res. Commun 2007;2:437-440). In addition, administration of exogenous FGF21 reduces serum TGs, insulin and glucose Kharitonenkov, 10 Shiyanova, Koester, Ford, Micanovic, Galbreath, Sandusky, Hammond, Moyers, Owens, Gromada, Brozinick, Hawkins, Wroblewski, Li, Mehrbod, Jaskunas, Shanafelt. J. Clin. Invest 2005;115:,1627-1635. Interestingly, the hepatic mRNA expression of FGF21 was doubled in mice treated with the SlclOa2 inhibitor (Fig. 5C). As expected, the hepatic mRNA level of CYP7A1 was increased in drug-treated mice (Fig. 5C). When the hepatic cholesterol and TG 15 content was analyzed no difference was seen between inhibitor-treated and control animal (Fig. 5D). To verify SlclOa2 blockade, the mRNA levels of the FXR target genes FGF15, SHP and IBABP were assayed in samples from the distal ileum; they were all significantly reduced in response to treatment as could be expected from a diminished influx of BAs into the distal ileum (Fig. 5E). The gene expression for FXR was unaltered in the distal ileum 20 following treatment with the Slc1 0a2 protein inhibitor (Fig. 5E). Inhibition of ileal Slc10a2 in ob/ob mice reduces SREBP1c and its target genes in the liver Since dysregulated SREBP1c has been reported to be a major determinant of the increased 25 lipogenic response in ob/ob liver, ultimately leading to reduced hepatic insulin sensitivity, the mRNA expression of hepatic SREBP1c was next analyzed and three of its target genes. In accordance with the reduced SREBP1c levels observed in S/c10a2-/-mice both under basal and SR diet challenge, markedly reduced mRNA levels of SREBP1c were observed in response to treatment (Fig. 6A). Moreover, the mRNA levels of the SREBP1 c liver target 30 genes ACC and FAS were strongly decreased, by 50% and 80%, respectively (Fig. 6A). However, the SCD1 mRNA level was not significantly altered compared to the vehicle treated controls (Fig. 6A). Altered expression of hepatic glucose metabolic genes in SlclOa2-inhibitor treated 35 ob/ob mice Next the expression levels of important enzymes for glucose handling in the livers of ob/ob mice treated with the compound of Example 14 was investigated. As seen in Fig. 6B, the WO 2012/064268 PCT/SE2011/051337 62 mRNA level for GK, the initial step in glycolysis, was increased in treated mice, in line with decreased blood glucose levels. However, in contrast, mRNA for the glycolytic gene LPK was reduced by 30% (Fig. 6B). A similar result for the LPK mRNA was also found in the S/c10a2-/- livers (Fig. 4D). Also, the expression of the gluconeogenic genes G6Pase and 5 PEPCK (Fig. 6B) was analyzed and found that they were both reduced in the inhibitor treated mice, in line with the finding of reduced blood glucose levels in these animals. Inhibition of Slc1Oa2 reduces Akt and Mekl/2 activity in ob/ob mouse liver To further study the molecular mechanisms underlying the observed changes in hepatic 10 gene transcription in the inhibitor treated ob/ob mice, the activity of major kinase signal pathways known to be important in hepatic regulation of both glucose metabolism and lipogenesis was examined. Akt has been demonstrated to be a crucial component in regulating the hepatic response to insulin and other circulating factors with capacity to favour glycolysis and lipogenesis, and inhibit luconeogenesis upon food intake. The induction of 15 SREBP 1c transcription by insulin is dependent on an Akt pathway Hegarty, Bobard, Hainault, Ferre, Bossard, Foufelle. Proc. Natl.Acad. Sci. USA. 2005;102:2:791-796. . When Akt activity was evaluated by phosphospecific antibodies, a reduced serine 473 phosphorylation was noted in liver lysates from inhibitor-treated animals (Fig. 7A). Another kinase pathway activated by the insulin receptor and the FGF receptor 4/ beta-Klotho 20 complex, known as the FGF15 receptors, is the Mekl/2 - Erkl/2 pathway. Interestingly, it was found that also these important kinases displayed reduced activation in the ob/ob livers upon SlclOa2 inhibitor treatment. Conclusions 25 Interruption of the enterohepatic circulation of bile acids (BA) increases cholesterol metabolism, thereby stimulating hepatic cholesterol synthesis from acetate. The subsequent reduction of the hepatic acetate pool may alter triglyceride (TG) and glucose metabolism. This was explored in mice genetically deficient of the ileal apical sodium BA transporter (SlclOa2) and in ob/ob mice treated with the compound of Example 14 an inhibitor of 30 SlclOa2. Plasma TG levels were reduced in SlclOa2 deficient mice, and when challenged with a sucrose-rich diet, they displayed a reduced response in hepatic TG production as observed from the mRNA levels for key enzymes in fatty acid synthesis, ACL, ACC, FAS, SCD1. This effect was paralleled by a diminished induction of mature SREBP1c. Consistently, pharmacologic inhibition of SIc10a2 in diabetic ob/ob mice reduced serum 35 glucose, insulin and TGs, as well as hepatic mRNA levels of SREBP1c and its target genes ACC and FAS. Moreover, when key metabolic signal transduction intermediates in the liver were investigated, it was found that the Mekl/2 -Erkl/2 pathway together with Akt were WO 2012/064268 PCT/SE2011/051337 63 blunted in ob/ob mice after treatment with the SlclOa2 inhibitor. It is concluded that abrogation of Slc1Oa2 reduces hepatic SREBP1c activity and serum TGs, and in the diabetic ob/ob model also reduces glucose and insulin levels. Hence, targeting Slc1Oa2 may be a strategy to treat hypertriglyceridemia and diabetes. 5 Example 17 The effect of compounds as disclosed herein give an increase of Glucagone-like peptide-1 10 (GLP1) also having an effect on the insulin secretion. In a double-blind, placebo-controlled study of 36 female patients with functional constipation randomized to placebo, 15 mg of the compound according to Example 14, or 20mg of the compound according to Example 14 was administered orally once daily for 14 consecutive 15 days, and the Glucagon-like peptide-1 (GLP-1) was assessed. For differences the model is simply anova for differences (analysis for variance) vs. treatment. Analyses for 15mg vs. Placebo, 20mg vs placebo, as well as analyses for non- placebo vs. placebo is statitistically significant separated. The results are presented in figure 8 20 Example 18 In a Phase llb, Double-blind, Randomized, Placebo-controlled, Multi-centre, Dose-finding, Efficacy and Safety of a Range of Doses of the substance according to Example 14 in Patients with Chronic Idiopathic Constipation in addition also have glucose values above Upper limit of normal (ULN), treated for 56 days. The patient was a male or non-pregnant 25 female 20 years of age and 580 years of age with body mass index (BMI) 18.5 but <35. When baseline value of glucose concentration was compared with end of treatment concentration the patients with higher values of glucose than ULN showed a significant reduction with 36 % at the dose 10 mg after 56 days of treatment with the compound of 30 Example 14 (Table 2, Fig 9). Contradictory patients with lower or equal value to ULN did not show any significant change with the substance according to Example 14. Table 2 Glucose concentrations in mg/dL 5ULN Placebo 10 mg n 26 25 Baseline Mean±SD 85.8±11.37 91.3±7.52 End of Mean±SD 90.7±16.62 90.6±12.96 WO 2012/064268 PCT/SE2011/051337 64 Treatment % change +6 % -1 % p-value 0.653 >ULN n 7 4 Baseline Mean±SD 128.1±14.87 129.8±7.4 End of Mean±SD 104.4±14.88 82±10.23 Treatment % change -18.5 % -36.9 p-value 0.013 p-values were obtained from an analysis of covariance with treatment group, demographic group and treatment-by-demographic group as fixed factors and baseline value as a covariate in the model. No adjustment for multiple comparisons was made. 5 Thus, the substance according to Example 14 were shown to lowering high glucose values in plasma, whereas normal values were almost not affected. Example 19 10 A formulation for delayed release of the BAT inhibitor having the following composition is prepared: Substance amount/capsule (mg) 15 IBAT inhibitor compound Example 14 10 Non pareil spheres 500 Ethyl cellulose 2 20 Hydroxypropylmethyl cellulose 10 Eudragit Li 00-55 CAS 25212 - 88 - 8 25 Triethylcitrate 2.4 The active drug is dissolved together with ethyl cellulose and hydroxypropyl cellulose in 25 ethanol 99 %. The mixture is then sprayed onto the non-pareil spheres in a fluidized bed apparatus. Thereafter, the pellets are dried and aerated to remove residual ethanol. The WO 2012/064268 PCT/SE2011/051337 65 Eudragit Li 00-55 dispersion with addition of triethyl citrate is then sprayed onto the drug beads in a fluidized bed apparatus. Subsequently, the coated beads are filled in hard gelatine capsules after drying and sieving. 5 Example 20 A formulation for delayed release of the IBAT inhibitor having the following composition can be prepared: Ingredient amount/tablet (mq) IBAT inhibitor compound 10 Example 14 10 Silicon dioxide 200 Povidone K-25 20 Eudragit FS30D CAS 26936 - 24 - 3 30 Microcrystalline cellulose 250 15 Sodium stearyl fumarate 5 The active drug is suspended in water and sprayed onto silicon dioxide cores of a predefined size in a fluidized bed apparatus. The drug pellets are dried in an oven at 40'C for 24 h. Thereafter, a layer of Povidone K-25 is applied on the beads from an ethanolic solution in a 20 fluidized bed apparatus. A final coat of Eudragit FS30D dispersion is applied thereafter in a fluidized bed. The coated beads are mixed with microcrystalline cellulose and sodium stearyl fumarate in a mixer and subsequently compressed to tablets. Example 21 25 An IBAT inhibitor - colesevelam combination tablet with immediate release of the IBAT inhibitor and colon release of the bile acid binder having the following composition is prepared: Ingredient amount/tablet (mq) Core 30 Colesevelam hydrochloride 400 Microcrystalline cellulose 150 Hydroxypropyl methyl cellulose 50 Colloidal silicon dioxide 10 Magnesium stearate 5 35 Colon release layer Eudragit FS30D 60 PlasACRYL T20 (CAS No 123-94-4) 6 WO 2012/064268 PCT/SE2011/051337 66 IBA T inhibitor layer IBAT inhibitor Example 14 7 Hydroxypropylmethyl cellulose 12 Croscarmellose sodium 6 5 Protective coating Hydroxypropylmethyl cellulose 12 Polyethylene glycol 2 10 Colesevelam hydrochloride, microcrystalline cellulose and colloidal silicon dioxide are mixed and granulated with hydroxypropyl methyl cellulose dissolved in water. The granules are dried and mixed with magnesium stearate and compressed to tablets. The EUDRAGIT FS30D dispersion and water is stirred into the PlasACRYL T20 (CAS number 123-94-4) and sprayed onto the core tablets using a suitable coating machine. The BAT inhibitor coating 15 suspension is prepared by mixing the BAT inhibitor, hydroxypropyl methyl cellulose and croscarmellose sodium in water and sprayed onto the tablet cores with the colon release layer using a suitable coating machine. Finally the protective coating solution of hydroxypropylmethyl cellulose and polyethylene glycol is sprayed onto the tablets using a suitable coating machine. 20 Example 22 A Colesevelam colon release tablet having the following composition is prepared: Ingredient amount /tablet (mq) 25 Core Colesevelam hydrochloride 400 Microcrystalline cellulose 150 Hydroxypropyl methyl cellulose 50 Colloidal silicon dioxide 10 30 Magnesium stearate 5 Colon release layer Amylose 30 Eudragit S100 60 Triethylcitrate 6 35 Glycerolmonostearate 3 WO 2012/064268 PCT/SE2011/051337 67 Colesevelam hydrochloride, microcrystalline cellulose and colloidal silicon dioxide are mixed and granulated with hydroxypropyl methyl cellulose dissolved in water. The granules are dried and mixed with magnesium stearate and compressed to tablets. Amylose, Eudragit 100, triethylcitrate and glycerolmonosterate are dissolved in suitable solvents and sprayed 5 onto the tablet cores using a suitable coating machine.

Claims (23)

1. Use of a compound of formula II R1 \/0 N 0~~S ' H N 0 S'A'NI HO R 2 wherein s M is -CH 2 or NH; R 1 is H or OH; and R2 is H, CH 3 , -CH 2 CH 3 , -CH 2 CH 2 CH 3 , -CH 2 CH 2 CH 2 CH 3 , -CH(CH 3 ) 2 , -CH 2 CH(CH 3 ) 2 , -CH(CH 3 )CH 2 CH 3 , -CH 2 OH, CH 2 0CH 3 , -CH(OH)CH 3 , -CH 2 SCH 3 , or -CH 2 CH 2 -S-CH 3 ; in the manufacture of a medicament for the prophylaxis or treatment of a glucose 10 utilization disorder.

2. The use according to claim 1, wherein the glucose utilization disorder is a disorder in which insulin resistance is involved.

3. The use according to claim 2, wherein the disorder is diabetes mellitus type 1.

4. The use according to claim 2, wherein the disorder is diabetes mellitus type 2. 15 5. The use according to any one of claims 1 to 4, wherein the compound of formula II is selected from the group consisting of: 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N (carboxymethyl)carbamoyl] benzyl} carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5 benzothiadiazepine; 20 1,1-Dioxo-3, 3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N'-((S)-1-carboxyethyl) carbamoyl] benzyl} carbamoylmethoxy)-2,3,4,5-tetrahydro-1,5-benzothiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N-((S)-1-carboxypropyl) carbamoyl]benzyl carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5-benzothiadiazepine; 69 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((R)- 1 -carboxy-2 methylthioethyl)carbamoyl]benzyl I carbamoylmethoxy)-2,3,4,5-tetrahydro- 1,2,5 benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((S)- 1-carboxypropyl) 5 carbamoyl] -4-hydroxybenzyl }carbamoylmethoxy)-2,3,4,5-tetrahydro- 1,2,5 benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((R)- 1 -carboxy-2 methylthio-ethyl)carbamoyl] -4-hydroxybenzyl I carbamoylmethoxy) -2,3,4,5-tetrahydro 1,2,5-benzothiadiazepine; 10 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((S)- 1-carboxy-2 methylpropyl)carbamoyl]benzyl I carbamoylmethoxy)-2,3,4,5-tetrahydro- 1,2,5 benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((S)- 1-carboxy-2-(R) hydroxypropyl)carbamoyl] -4-hydroxybenzyl I carbamoylmethoxy)-2,3,4,5-tetrahydro 15 1,2,5-benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((S)- 1-carboxybutyl) carbamoyl] -4-hydroxybenzyl Icarbamoylmethoxy)-2,3,4,5-tetrahydro- 1,2,5 benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-c-[N-((S)- 1-carboxyethyl) 20 carbamoyl]benzyl I carbamoylmethoxy)-2,3,4,5-tetrahydro- 1,2,5-benzothiadiazepine 1,1-dioxo-3, 3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N'-((S)-1 carboxypropyl)carbamoyl]-4-hydroxybenzylI carbamoylmethoxy)-2,3,4,5-tetrahydro-1,

5-benzothiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N-((S)-1 25 carboxyethyl)carbamoyl]-4-hydroxybenzylIcarbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5 benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N-((S)-1-carboxy-2 methylpropyl)carbamoyl]-4-hydroxybenzylIcarbamoylmethoxy)-2,3,4,5-tetrahydro 1,2,5-benzothiadiazepine; and 30 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{ (R)-l'-phenyl-l'-[N' (carboxymethyl) carbamoyl]methylIcarbamoylmethoxy)-2,3,4,5-tetrahydro-1,5 benzothiazepine. 70

6. The use according to any one of claims 1 to 4, wherein the compound of formula II is 1,1-dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N-((S)-1-carboxypropyl) carbamoyl]-4-hydroxybenzyl carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5 benzothiadiazepine. 5

7. The use according to any one of claims 1 to 4, wherein the compound of formula II is 1,1-dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-1'-phenyl-l'-[N' (carboxymethyl)carbamoyl] methyl} carbamoylmethoxy)-2,3,4,5-tetrahydro-1,5 benzothiazepine.

8. Use of a pharmaceutical composition comprising a compound of formula II R1 0 S N \ / 0 HO R 2 10 wherein M is -CH 2 or NH; R 1 is H or OH; and R2 is H, CH 3 , -CH 2 CH 3 , -CH 2 CH 2 CH 3 , -CH 2 CH 2 CH 2 CH 3 , -CH(CH 3 ) 2 , -CH 2 CH(CH 3 ) 2 , 15 -CH(CH 3 )CH 2 CH 3 , -CH 2 OH, CH 2 0CH 3 , -CH(OH)CH 3 , -CH 2 SCH 3 , or -CH 2 CH 2 -S-CH 3 ; in the manufacture of a medicament for the prophylaxis or treatment of a glucose utilization disorder. 71

9. Use of a combination comprising (i) a compound of formula II R1 \/0 N 0~~S ' H N 0 S'A'NI HO R 2 wherein s M is -CH 2 or NH; R 1 is H or OH; and R2 is H, -CH 3 , -CH 2 CH 3 , -CH 2 CH 2 CH 3 , -CH 2 CH 2 CH 2 CH 3 , -CH(CH 3 ) 2 , -CH 2 CH(CH 3 ) 2 , -CH(CH 3 )CH 2 CH 3 , -CH 2 OH, CH 2 0CH 3 , -CH(OH)CH 3 , -CH 2 SCH 3 , or -CH 2 CH 2 -S-CH 3 ; and 10 (ii) at least one other active substance selected from an IBAT inhibitor; an enteroendocrine peptide or enhancer thereof; a dipeptidyl peptidase-IV inhibitor; a biguanidine; an incretin mimetic; a thiazolidinone; a PPAR agonist; a HMG Co-A reductase inhibitor; a bile acid binder; and a TGR5 receptor modulator; or a pharmaceutically acceptable salt of any one of said active substances, 15 in the manufacture of a medicament for the prophylaxis or treatment of a glucose utilization disorder.

10. The use according to claim 9, wherein the compound of formula (II) is selected from the group consisting of: 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N 20 (carboxymethyl)carbamoyl]-benzyl carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5 benzothiadiazepine; 1,1-Dioxo-3, 3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N'-((S)-1 carboxyethyl)carbamoyl] benzyl carbamoylmethoxy)-2,3,4,5-tetrahydro-1,5 benzothiazepine; 72 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((S)- 1-carboxypropyl) carbamoyl]benzyl I carbamoylmethoxy)-2,3,4,5-tetrahydro- 1,2,5-benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((R)- 1 -carboxy-2 methylthioethyl)carbamoyl]benzyl I carbamoylmethoxy)-2,3,4,5-tetrahydro- 1,2,5 5 benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((S)- 1-carboxypropyl) carbamoyl] -4-hydroxybenzyl }carbamoylmethoxy)-2,3,4,5-tetrahydro- 1,2,5 benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((R)- 1 -carboxy-2 10 methylthio-ethyl)carbamoyl] -4-hydroxybenzyl I carbamoylmethoxy) -2,3,4,5-tetrahydro 1,2,5-benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((S)- 1-carboxy-2 methylpropyl)carbamoyl]benzyl I carbamoylmethoxy)-2,3,4,5-tetrahydro- 1,2,5 benzothiadiazepine; 15 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((S)- 1-carboxy-2-(R) hydroxypropyl)carbamoyl] -4-hydroxybenzyl I carbamoylmethoxy)-2,3,4,5-tetrahydro 1,2,5-benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((S)- 1-carboxybutyl) carbamoyl] -4-hydroxybenzyl Icarbamoylmethoxy)-2,3,4,5-tetrahydro- 1,2,5 20 benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((S)- 1-carboxyethyl) carbamoyl]benzyl I carbamoylmethoxy)-2,3,4,5-tetrahydro- 1,2,5-benzothiadiazepine; 1,1-dioxo-3, 3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N'-((S)-1-carboxypropyl) carbamoyl]-4-hydroxybenzylIcarbamoylmethoxy)-2,3,4,5-tetrahydro-1,5 25 benzothiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N-((S)-1 carboxyethyl)carbamoyl]-4-hydroxybenzylIcarbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5 benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N-((S)-1-carboxy-2 30 methylpropyl)carbamoyl]-4-hydroxybenzylIcarbamoylmethoxy)-2,3,4,5-tetrahydro 1,2,5-benzothiadiazepine; and 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{ (R)-1'-phenyl-l'-[N' (carboxymethyl) carbamoyl]methylIcarbamoylmethoxy)-2,3,4,5-tetrahydro-1,5 benzothiazepine. 73

11. The use according to claim 9, wherein the compound of formula (II) is 1,1-dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N-((S)-1-carboxypropyl) carbamoyl]-4-hydroxybenzyllcarbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5 benzothiadiazepine. 5

12. The use according to claim 9, wherein the compound of formula (II) is 1,1-dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-1'-phenyl-l'-[N' (carboxymethyl)carbamoyl] methyl} carbamoylmethoxy)-2,3,4,5-tetrahydro-1,5 benzothiazepine.

13. The use according to any one of claims 9 to 12, wherein the at least one other 10 active substance is a bile acid binder.

14. The use according to claim 13, wherein the bile acid binder is cholestyramine, cholestipol or colesevelam.

15. The use according to any one of claims 9 to 12, wherein the at least one other active substance is a HMG Co-A reductase inhibitor. 15

16. The use according to claim 15, wherein the HMG Co-A reductase inhibitor is a statin.

17. A method for the treatment or prophylaxis of a glucose utilization disorder, wherein a compound according to formula II R1 \/0 HH N 0'~ 0 oN HO R 2 20 wherein M is -CH 2 or NH; 74 R 1 is H or OH; and R2 is H, CH 3 , -CH 2 CH 3 , -CH 2 CH 2 CH 3 , -CH 2 CH 2 CH 2 CH 3 , -CH(CH 3 ) 2 , -CH 2 CH(CH 3 ) 2 , -CH(CH 3 )CH 2 CH 3 , -CH 2 OH, CH 2 0CH 3 , -CH(OH)CH 3 , -CH 2 SCH 3 , or -CH 2 CH 2 -S-CH 3 ; is administered to a subject in need of such treatment. 5

18. The method according to claim 17, wherein the glucose utilization disorder is a disorder in which insulin resistance is involved.

19. The method according to claim 18, wherein the disorder is diabetes mellitus type 1.

20. The method according to claim 18, wherein the disorder is diabetes mellitus 10 type 2.

21. The method according to any one of claims 17 to 20, wherein the compound of formula 11 is selected from the group consisting of: 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{ (R)-a-[N (carboxymethyl)carbamoyl] benzyl carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5 15 benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8- (N-{(R)-a-[N'-((S)-1-carboxyethyl) carbamoyl] benzyl} carbamoylmethoxy)-2,3,4,5-tetrahydro-1,5-benzothiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N-((S)-1-carboxypropyl) carbamoyl]benzyl carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5-benzothiadiazepine; 20 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N-((R)-1-carboxy-2 methylthioethyl)carbamoyl]benzyl carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5 benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N-((S)-1-carboxypropyl) carbamoyl]-4-hydroxybenzyl carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5 25 benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N-((R)-1-carboxy-2 methylthio-ethyl)carbamoyl]-4-hydroxybenzyl carbamoylmethoxy)-2,3,4,5-tetrahydro 1,2,5-benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N-((S)-1-carboxy-2 30 methylpropyl)carbamoyl]benzyl carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5 benzothiadiazepine; 75 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((S)- 1-carboxy-2-(R) hydroxypropyl)carbamoyl] -4-hydroxybenzyl I carbamoylmethoxy)-2,3,4,5-tetrahydro 1,2,5-benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((S)- 1-carboxybutyl) 5 carbamoyl] -4-hydroxybenzyl }carbamoylmethoxy)-2,3,4,5-tetrahydro- 1,2,5 benzothiadiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N- I (R)-a-[N-((S)- 1-carboxyethyl) carbamoyl]benzyl I carbamoylmethoxy)-2,3,4,5-tetrahydro- 1,2,5-benzothiadiazepine; 1,1-dioxo-3, 3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N'-((S)-1-carboxypropyl) 10 carbamoyl]-4-hydroxybenzyl carbamoylmethoxy)-2,3,4,5-tetrahydro-1,5 benzothiazepine; 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N-((S)-1 carboxyethyl)carbamoyl]-4-hydroxybenzyl carbamoylmethoxy)-2,3,4,5-tetrahydro-1,2,5 benzothiadiazepine; 15 1,1-Dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N-((S)-1-carboxy-2 methylpropyl)carbamoyl]-4-hydroxybenzyl carbamoylmethoxy)-2,3,4,5-tetrahydro 1,2,5-benzothiadiazepine; and 1,1-Dioxo-3, 3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-1'-phenyl-l'-[N' (carboxymethyl) carbamoyl]methyl carbamoylmethoxy)-2,3,4,5-tetrahydro-1,5 20 benzothiazepine.

22. The method according to any one of claims 17 to 20, wherein the compound of formula II is 1,1-dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-I(R)-a-[N-((S)-1 carboxypropyl) carbamoyl]-4-hydroxybenzyl carbamoylmethoxy)-2,3,4,5-tetrahydro 1,2,5-benzothiadiazepine. 25

23. The method according to any one of claims 17 to 20, wherein the compound of formula II is 1,1-dioxo-3,3-dibutyl-5-phenyl-7-methylthio-8-(N-{(R)-1'-phenyl-l'-[N' (carboxymethyl)carbamoyl] methyl} carbamoylmethoxy)-2,3,4,5-tetrahydro-1,5 benzothiazepine. Albireo AB 30 Patent Attorneys for the Applicant/Nominated Person SPRUSON & FERGUSON