AU2011259331A1 - Method for producing biofuel - Google Patents
Method for producing biofuel Download PDFInfo
- Publication number
- AU2011259331A1 AU2011259331A1 AU2011259331A AU2011259331A AU2011259331A1 AU 2011259331 A1 AU2011259331 A1 AU 2011259331A1 AU 2011259331 A AU2011259331 A AU 2011259331A AU 2011259331 A AU2011259331 A AU 2011259331A AU 2011259331 A1 AU2011259331 A1 AU 2011259331A1
- Authority
- AU
- Australia
- Prior art keywords
- biofuel
- culture solution
- photosynthetic microorganisms
- production method
- fats
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000002551 biofuel Substances 0.000 title claims abstract description 49
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 74
- 244000005700 microbiome Species 0.000 claims abstract description 65
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 62
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 54
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 27
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 27
- 238000006243 chemical reaction Methods 0.000 claims abstract description 24
- 150000001720 carbohydrates Chemical class 0.000 claims abstract description 22
- 238000000605 extraction Methods 0.000 claims abstract description 21
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 7
- 239000003921 oil Substances 0.000 claims description 42
- 239000003925 fat Substances 0.000 claims description 34
- 238000012258 culturing Methods 0.000 claims description 31
- 235000014593 oils and fats Nutrition 0.000 claims description 31
- 235000014633 carbohydrates Nutrition 0.000 claims description 20
- 238000004062 sedimentation Methods 0.000 claims description 19
- 238000009833 condensation Methods 0.000 claims description 18
- 230000005494 condensation Effects 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 17
- 238000001035 drying Methods 0.000 claims description 14
- 238000000926 separation method Methods 0.000 claims description 13
- 238000002407 reforming Methods 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 9
- 230000029553 photosynthesis Effects 0.000 abstract description 16
- 238000010672 photosynthesis Methods 0.000 abstract description 16
- 239000002028 Biomass Substances 0.000 abstract description 11
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 6
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 80
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- 235000019197 fats Nutrition 0.000 description 30
- 239000007789 gas Substances 0.000 description 24
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 15
- 239000001301 oxygen Substances 0.000 description 15
- 229910052760 oxygen Inorganic materials 0.000 description 15
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 12
- 239000000446 fuel Substances 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
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- 235000008935 nutritious Nutrition 0.000 description 5
- 238000000638 solvent extraction Methods 0.000 description 5
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 4
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 4
- 241001293481 Trebouxiophyceae Species 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- RMRCNWBMXRMIRW-BYFNXCQMSA-M cyanocobalamin Chemical compound N#C[Co+]N([C@]1([H])[C@H](CC(N)=O)[C@]\2(CCC(=O)NC[C@H](C)OP(O)(=O)OC3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)C)C/2=C(C)\C([C@H](C/2(C)C)CCC(N)=O)=N\C\2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O RMRCNWBMXRMIRW-BYFNXCQMSA-M 0.000 description 4
- 239000011261 inert gas Substances 0.000 description 4
- 239000003960 organic solvent Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000195628 Chlorophyta Species 0.000 description 3
- 241000195634 Dunaliella Species 0.000 description 3
- 241000195623 Euglenida Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 241001148696 Stichococcus Species 0.000 description 3
- 241000192707 Synechococcus Species 0.000 description 3
- 239000004164 Wax ester Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 230000005484 gravity Effects 0.000 description 3
- 238000005984 hydrogenation reaction Methods 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 239000003350 kerosene Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000002918 waste heat Substances 0.000 description 3
- 235000019386 wax ester Nutrition 0.000 description 3
- 241000192698 Aphanocapsa Species 0.000 description 2
- 241000195585 Chlamydomonas Species 0.000 description 2
- 241000195598 Chlamydomonas moewusii Species 0.000 description 2
- 241000192700 Cyanobacteria Species 0.000 description 2
- 241000159506 Cyanothece Species 0.000 description 2
- 241000305506 Desmodesmus Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000196305 Nannochloris Species 0.000 description 2
- 241000195646 Parachlorella kessleri Species 0.000 description 2
- 241000192608 Phormidium Species 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 241000195663 Scenedesmus Species 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 241001512067 Symploca Species 0.000 description 2
- 241000192584 Synechocystis Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 229910052786 argon Inorganic materials 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 229960002104 cyanocobalamin Drugs 0.000 description 2
- 235000000639 cyanocobalamin Nutrition 0.000 description 2
- 239000011666 cyanocobalamin Substances 0.000 description 2
- 239000000428 dust Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- -1 ester compounds Chemical class 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 239000003208 petroleum Substances 0.000 description 2
- 241000196307 prasinophytes Species 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000004449 solid propellant Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 2
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 2
- 241000192542 Anabaena Species 0.000 description 1
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- 101100283604 Caenorhabditis elegans pigk-1 gene Proteins 0.000 description 1
- 208000003643 Callosities Diseases 0.000 description 1
- 235000016401 Camelina Nutrition 0.000 description 1
- 244000197813 Camelina sativa Species 0.000 description 1
- 241000719290 Cephaleuros Species 0.000 description 1
- 241000195597 Chlamydomonas reinhardtii Species 0.000 description 1
- 240000009108 Chlorella vulgaris Species 0.000 description 1
- 235000007089 Chlorella vulgaris Nutrition 0.000 description 1
- 241000195658 Chloroidium saccharophilum Species 0.000 description 1
- 241000206751 Chrysophyceae Species 0.000 description 1
- 241001472322 Colacium Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
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- 241001497555 Desmodesmus subspicatus Species 0.000 description 1
- 241000199914 Dinophyceae Species 0.000 description 1
- 241000195633 Dunaliella salina Species 0.000 description 1
- 241000195632 Dunaliella tertiolecta Species 0.000 description 1
- 241000195619 Euglena gracilis Species 0.000 description 1
- 241000006367 Euglenales Species 0.000 description 1
- 241000224472 Eustigmatophyceae Species 0.000 description 1
- 241001646653 Galdieria Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 241000206759 Haptophyceae Species 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 241000221089 Jatropha Species 0.000 description 1
- 241000508185 Lobochlamys segnis Species 0.000 description 1
- 241001148150 Mastigocladus Species 0.000 description 1
- 241001647400 Mastigocladus laminosus Species 0.000 description 1
- 241001375804 Mastigophora Species 0.000 description 1
- 241001288851 Nannochloris bacillaris Species 0.000 description 1
- 235000019482 Palm oil Nutrition 0.000 description 1
- 241001036353 Parachlorella Species 0.000 description 1
- 241000384537 Phacus Species 0.000 description 1
- 241000206618 Porphyridium Species 0.000 description 1
- 244000249201 Scenedesmus obliquus Species 0.000 description 1
- 235000007122 Scenedesmus obliquus Nutrition 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- 241001453296 Synechococcus elongatus Species 0.000 description 1
- 241000192589 Synechococcus elongatus PCC 7942 Species 0.000 description 1
- 241001464792 Synechococcus lividus Species 0.000 description 1
- 241000192560 Synechococcus sp. Species 0.000 description 1
- 241000196321 Tetraselmis Species 0.000 description 1
- 241001313699 Thermosynechococcus elongatus Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003637 basic solution Substances 0.000 description 1
- 239000003225 biodiesel Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 150000001722 carbon compounds Chemical class 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- 239000001752 chlorophylls and chlorophyllins Substances 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000006477 desulfuration reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000000295 fuel oil Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 150000004668 long chain fatty acids Chemical class 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 239000002540 palm oil Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 238000011946 reduction process Methods 0.000 description 1
- 101150055666 sox6 gene Proteins 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
-
- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G, C10K; LIQUEFIED PETROLEUM GAS; ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE OR UNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; FIRELIGHTERS
- C10L1/00—Liquid carbonaceous fuels
- C10L1/02—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
-
- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10G—CRACKING HYDROCARBON OILS; PRODUCTION OF LIQUID HYDROCARBON MIXTURES, e.g. BY DESTRUCTIVE HYDROGENATION, OLIGOMERISATION, POLYMERISATION; RECOVERY OF HYDROCARBON OILS FROM OIL-SHALE, OIL-SAND, OR GASES; REFINING MIXTURES MAINLY CONSISTING OF HYDROCARBONS; REFORMING OF NAPHTHA; MINERAL WAXES
- C10G3/00—Production of liquid hydrocarbon mixtures from oxygen-containing organic materials, e.g. fatty oils, fatty acids
-
- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G, C10K; LIQUEFIED PETROLEUM GAS; ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE OR UNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; FIRELIGHTERS
- C10L1/00—Liquid carbonaceous fuels
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
- C11C3/00—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
-
- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10G—CRACKING HYDROCARBON OILS; PRODUCTION OF LIQUID HYDROCARBON MIXTURES, e.g. BY DESTRUCTIVE HYDROGENATION, OLIGOMERISATION, POLYMERISATION; RECOVERY OF HYDROCARBON OILS FROM OIL-SHALE, OIL-SAND, OR GASES; REFINING MIXTURES MAINLY CONSISTING OF HYDROCARBONS; REFORMING OF NAPHTHA; MINERAL WAXES
- C10G2300/00—Aspects relating to hydrocarbon processing covered by groups C10G1/00 - C10G99/00
- C10G2300/10—Feedstock materials
- C10G2300/1011—Biomass
- C10G2300/1014—Biomass of vegetal origin
-
- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10G—CRACKING HYDROCARBON OILS; PRODUCTION OF LIQUID HYDROCARBON MIXTURES, e.g. BY DESTRUCTIVE HYDROGENATION, OLIGOMERISATION, POLYMERISATION; RECOVERY OF HYDROCARBON OILS FROM OIL-SHALE, OIL-SAND, OR GASES; REFINING MIXTURES MAINLY CONSISTING OF HYDROCARBONS; REFORMING OF NAPHTHA; MINERAL WAXES
- C10G2300/00—Aspects relating to hydrocarbon processing covered by groups C10G1/00 - C10G99/00
- C10G2300/40—Characteristics of the process deviating from typical ways of processing
- C10G2300/4043—Limiting CO2 emissions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P30/00—Technologies relating to oil refining and petrochemical industry
- Y02P30/20—Technologies relating to oil refining and petrochemical industry using bio-feedstock
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P30/00—Technologies relating to oil refining and petrochemical industry
- Y02P30/40—Ethylene production
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Liquid Carbonaceous Fuels (AREA)
- Production Of Liquid Hydrocarbon Mixture For Refining Petroleum (AREA)
Abstract
Disclosed are a series of processes for producing a biofuel, which include a technique wherein carbon dioxide serving as a carbon source is converted into a biomass by photosynthesis by photosynthetic microorganisms and the production of a biofuel is carried out after that. Specifically disclosed is a method for producing a biofuel, which is characterized by comprising: a culture step (S1) wherein photosynthetic microorganisms that accumulate a fat or oil and a carbohydrate in the cells thereof are cultured in a broth; a fat or oil conversion step (S2) wherein the carbohydrate accumulated in the cells of the photosynthetic microorganisms, which have been cultured in the broth, is converted into a fat or oil; an extraction step (S3) wherein the oil or fat is extracted from the cells of the photosynthetic microorganisms; and a modification step (S4) wherein the extracted fat or oil is modified.
Description
Production Method For Biofuel BACKGROUND OF THE INVENTION FIELD OF THE INVENTION [00011 The present invention relates to a production method for a biofuel to produce the biofuel. [0002] The biofuel is a fuel produced from a raw material derived from biomass. If the biomass has a photosynthesis capability as ordinary plants have, it is possible to produce a fuel that has a smaller effect on the environment, because oils, fats and carbohydrates produced through the biomass from light energy, carbon-dioxide are raw materials for the biofuel. There are biofuels such as a bioethanol produced through alcohol fermentation of saccharified carbohydrate and a biodiesel oil and a biojetfuel which are produced from neutral lipids such as waxesters and triglycerides [00031 Although soy beans, corns and palms have been well known to be used for raw materials for the biofuel, there is an issue that using edible crops such as these crops leads to shortage of foods. Alternatively, non-edible crops such as jatropha and camelina are produced for the biofuel and there is a problem with these crops having a low yield for a unit area. [00041 On the other hand, it is known that photosynthetic microorganisms and protozoa living in many lakes and marshes have the same photosynthesis capability as plants have and synthesize oils, fats and carbohydrates from water and carbon-dioxide and store in their cells an amount of oils, fats and carbohydrates, which corresponds to several tens percents of a dry weight of the photosynthetic microorganisms and protozoa. The photosynthetic microorganisms and protozoa are capable of producing a larger amount of oils, 1 fats and carbohydrates than plants do and produce a more than ten times larger amount of oils, fats and carbohydrates for a unit area than palm which is said to be capable of producing a large amount of palm oils. [0005] A patent document 1 describes fixing carbon-dioxide by culturing algae which are capable of photosynthesis by radiating the artificial light whose wave length and light intensity are artificially adjusted. The patent document 1 further describes a means, a method and a cultivation device to produce a target substance by fixing carbon-dioxide. Moreover the patent document 1 proposes fixing carbon-dioxide with photosynthetic microorganisms and making use of the fixed carbon-dioxide as a basic fuel for the biofuel as an example. PRIOR ART DOCUMENT PATENT DOCUMENT [0006] Patent Document 1 : JP2010-057485A SUMMARY OF THE INVENTION OBJECTIVE TO BE ACHIEVED BY THE INVENTION [0007] However the patent document 1 does not disclose anything about how a biofuel is produced from the fixed carbon-dioxide. When the carbon-dioxide gas is reduced by making use of photosynthesis capability of microorganisms, the weight of the biomass increases in proportion to the amount of the fixed carbon dioxide and it is difficult to make an industrial use of this invention if the biomass with the increased weight is effectively used. [0008] The present invention is intended to provide a biofuel production method inclusive of a series of technologies to convert carbon-dioxide which is a carbon source to the biomass through photosynthesis by photosynthetic microorganisms 2 and subsequently produce a biofuel. MEANS TO ACHIEVE THE OBJECTIVE [0009] In order to achieve the objective above mentioned, the production method for biofuel of the present invention comprises a culturing process of culturing in a culture solution photosynthetic microorganisms which store oils, fats and carbohydrates in cells of the photosynthetic microorganisms, an oil and fat conversion process of converting the carbohydrates stored in the cells of the photosynthetic microorganisms cultured in the culture apparatus to oils and fats, an extraction process of extracting the oils and fats out of the cells of the photosynthetic microorganisms, and a reforming process to reform the extracted oils and fats. [0010] According to the production method for biofuel of the present invention, the photosynthetic microorganisms are cultured in the culturing process, carbon dioxide which is a carbon source is converted through photosynthesis by the photosynthetic microorganisms cultured in the culture solution to oils, fats and carbohydrates which are stored in cells of the photosynthetic microorganisms and the stored carbohydrates are converted to oils and fats. As a result, a larger amount of oils and fats than produced in any of the current production methods is efficiently produced. In addition, according to the production method for biofuel of the present invention, oils and fats are subsequently extracted in an extraction process and the extracted oils, fats are reformed in a reforming process that follows the extraction process and biofuel is produced. EFFECT OF THE INVENTION [0011] The present invention is intended to provide a biofuel production method inclusive of a series of technologies to convert carbon-dioxide which is a carbon source to the biomass through photosynthesis by photosynthetic microorganisms 3 and subsequently produce a biofuel. Moreover the present invention enables producing a larger amount of a biofuel than any of the current biofuel production methods, because it has a production process inclusive of an oil and fat conversion process. BRIEF DESCRIPTION OF DRAWINGS [0012] Fig.1 is a flowchart explaining an embodiment for a biofuel production method of the present invention. Fig.2 is a block diagram explaining an embodiment for the biofuel production apparatus. DETAILED DESCRIPTION OF THE EMBODIMENTS [00131 Hereinafter an embodiment of a biofuel production method of the present invention is explained with reference to Fig.1. [0014] As shown in Fig.1, the biofuel production method of the present invention includes a culturing process S1, an oil and fat conversion process S2, an extraction process S3 and a reforming process S4. [0015] The culturing process S1 to be performed at first is a process to culture photosynthetic microorganisms which store oils, fats and carbohydrates in their cells in a culture solution. Euglena is considered to be photosynthetic microorganisms to be used for the present invention. Euglena includes Euglena gracilis which belongs to a group of flagellates and is well known as a motile alga. Most of the Euglena have chlorophylls and live autotrophically through photosynthesis and there are some of the Euglena which are of a predator type or absorb nourishment from other organisms. The Euglena is classified in both the zoology and the botany. In the 4 zoology, a Euglenida, which is one of orders belonging to a Mastigophora and a Phytomastigophora in Protozoa, consists of three suborders, Eulenoidina, Peranemoidina and Petalomonadoidina. The Eulenoidina includes Genera, Euglena, Trachelemonas, Strombonas, Phacus, Lepocinelis and Colacium. In the botany, a Euglenales, which belongs to a Euglenophyceae in a Euglenophyta, includes genera such as the Euglena, as is the same way as in the zoology. Besides the Euglena used for the present invention may be one or more of Cyanobacteria, Green algae and Trebouxiophyceae, Bangiophyceae, Prasinophyceae, Bacillasiophyceae, Coccolithophorid, Dinophyceae, Eustigmatophyceae and Chrysophyceae. [0016] Examples of the Cyanobacteria for the present invention are Chroococcacae, Stigonematacae, Mastigocladacae and Oscillatroriacae and other examples are Synechococcus such as Synechococcus lividus and Synechococcus elongatus, Synechocystis such as Synechocystis minervae, Mastigocladus such as Mastigocladus laminosus, Phormidium such as Phormidium laminosus, Symploca such as Symploca thermals, Aphanocapsa such as Aphanocapsa thermalis and Fisherella. Furthermore Anabanena variabilis ATCC 29413 which belongs to genus Anabaena, Cyanothece sp. ATCC 51142 which belongs to genus Cyanothece, Synechococcus sp. PCC 7942 which belongs to genus Synechococcus, and Anacystis nidulans and Thermosynechococcus elongatus which belong to Anacystis may be used for the present invention. [0017] Examples of green algae and Trebouxiophyceae for the present invention are Cephaleuros such as Chlorella inclusive of Parachlorella separated from Chlorella according to phylogenesis, Chlamydomonas, Dunaliella, Scenedesmus, Botryococcus, Stichococcus, Nannochloris and Desmodesmus. To be specific, examples of green algae and Trebouxiophyceae for the present invention are Chlorella vulgaris and Chlorella saccharophila 5 included in Chlorella, Dunaliella such as Dunaliella salina, Dunaliella tertiolecta and Parachlorella kessleri (Chlorella kessleri) whose basic properties like photosynthesis are the same as Chlorella and Dunaliella and which are classified in Trebouxiophyceae according to the molecular phylogenic analysis, for example. In addition, there are such other examples for the present invention as Chlamydomonas reinhardtii, Chlamydomonas moewusii, Chlamydomonas eugametos and Chlamydomonas segnis which belong to Chlamydomonas, Scenedesmus obliquus which belongs to Scenedesmus, Stichococcus ampliformis which belongs to Stichococcus, Nannochloris bacillaris which belongs to Nannochloris and Desmodesmus subspicatus which belongs to Desmodesmus. [0018] Furthermore, an example of Prasinophyceae for the present invention is Tetraselmis and examples of Bacillasiophyceae for the present invention are Cyanidioschyzon, Cyanidium, Galdieria and Porphyridium. It should be noted that any microorganism may be used for the present invention as long as it is capable of producing oils, fats and carbohydrates, storing them in its cells and converting the stored carbohydrates to oils and fats and that the microorganisms to be used for the present invention should not be restricted to those above mentioned. [0019] Though culturing photosynthetic microorganisms in the culturing process S1 may be carried out in the atmosphere, it is preferable to intentionally introduce the carbon-dioxide gas in the culture solution to increase the concentration of the carbon-dioxide dissolved in the culture solution to a higher one than the one for the condition in which the culture solution is just exposed to the atmosphere and increase the amount of oils, fats and carbohydrates that are produced through photosynthesis. For example, the carbon-dioxide gas discharged from factories and burning facilities may be used to intentionally introduce the carbon-dioxide gas in the culture solution. If the discharged carbon-dioxide gas is used, it is better to remove dust particles, NOx and SOx 6 included in the exhaust gas through a dust collector, a denitration apparatus and a desulfuration apparatus in advance. [0020] A liquid depth of the culture solution in the culturing process S1 is preferably equal to or less than 50 cm. More preferably the liquid depth of the culture solution in the culturing process S1 is equal to or less than 30 cm. If the liquid depth is kept as above mentioned, the culture solution is well stirred in the up-down direction (the upper liquid is frequently replaced with the lower liquid) when the photosynthetic microorganisms proliferate as the culturing progresses and the photosynthesis is being performed efficiently. [0021] When Euglena are used as the photosynthetic microorganism, a culture medium to which a nitrogen source, a phosphorus source and minerals are added, such as a modified Cramer-Myers culture medium (including (NH 4
)
2 HP0 4 1.Og/L,
KH
2
PO
4 1.0g/L, MgSO 4 -7H20 0.2g/L, CaCl 2 -2H 2 0 0.02g/L, Fe 2
(SO
2 )s-7H20 3 mg/L, MnCl 2 -4H20 1.8 mg/L, CoSO 4 -7H20 1.5 mg/L, ZnSO 4 -7H20 0.4 mg/L, Na 2 MoO 4 -2H 2 0 0.2 mg/L, CuSO 4 -5H 2 0 0.02 g/L, thiamin chloride(vitamin B 1 ) 0.1 mg/L, and cyanocobalamin(vitaminB12) and with pH3.5) may be used. Any of
(NH
4
)
2 HS0 4 and NH 3 aq may be used instead of (NH 4
)
2 HP0 4 . Any culture medium may be used for the present invention if it is fit for the photosynthetic microorganism to be used. The culture medium is not limited to the above mentioned one. [0022] A pH of the culture solution is preferably between 2 and 6 and more preferably between 2 and 4.5. If the culture solution is acidic with its ph value kept as indicated above, the photosynthetic microorganism is capable of proliferating so well as to prevail over the other organisms, which enable suppressing contamination. As a result, a batch culture method as well as a continuous culture method may be used for the present invention. [0023] The pH of the culture solution is appropriately adjusted with powder 7 reagents and reagent solutions. One of the power reagents used for this pH adjustment may be sodium bicarbonate, and such acidic solutions as a sulfuric acid solution and an acetic acid solution and such a basic solution as a sodium hydroxide solution are examples of the reagent solutions. [0024] The oil and fat conversion step S2 that follows the step S1 is a step to convert the carbohydrates stored in cells of the photosynthetic microorganisms which have been cultured in the culture solution to oils and fats. The photosynthetic microorganisms stores oils and fats in their cells for self-defense when kept under anaerobic condition. Therefore, one of the methods for converting the carbohydrates stored in the photosynthetic microorganisms is, for example, to keep the culture solution in which the photosynthetic microorganisms have been cultured under anaerobic condition. In the present invention the anaerobic atmosphere refers to an atmosphere where there is no oxygen or less oxygen than in the atmosphere. The anaerobic condition is created according the following process. After the photosynthetic microorganisms are condensed in a condensation process S12 (sedimentation condensation and centrifugal separation), the photosynthetic microorganisms are kept in a closed space such as a closed container and a pipe, into which neither light nor oxygen comes, to prevent the photosynthetic microorganisms from producing oxygen through photosynthesis. Then oxygen in the closed space is consumed by the photosynthetic microorganisms respiration. As a result, the anaerobic condition is created. There may be other processes to create the anaerobic condition and the process to create the anaerobic condition is not limited to the above mentioned one. For example, the anaerobic condition is created by purging a space in which the photosynthetic microorganisms are kept with an inert gas like argon to remove oxygen. [0025] In the present invention the condensation process S12 is preferably carried out before the oil and fat conversion process S2, that is, between the oil and fat conversion process S2 and the culturing process S1. During the 8 condensation process S12, the photosynthetic microorganism, in whose cells oils, fats and carbohydrates are stored, are condensed and part of the culture solution that is not needed is removed. As a result, the subsequent process is easily carried out and this condensation process S12 enables creating the anaerobic atmosphere earlier because the oxygen dissolved in the condensed culture solution is completely consumed earlier through the photosynthesis by the photosynthetic microorganisms owing to the less culture solution left after the part of the culture solution is removed. Furthermore, the oil and fat conversion process progresses faster. [0026] In the condensation process S12, the culture solution is condensed preferably through the sedimentation condensation followed by the centrifugal separation process. Here the sedimentation condensation refers to having photosynthetic microorganism condensed through a natural sedimentation process such as leaving a solution inclusive of the photosynthetic microorganisms for 6 to 36 hours. Condensation through the centrifugal separation process is to have photosynthetic microorganisms condensed by a centrifugal force on a centrifugal machine. Performing the centrifugal separation process on a solution inclusive of photosynthetic microorganisms, the solution is separated into a heavy solution with a higher specific gravity inclusive of the photosynthetic microorganisms and a light solution of a supernatant liquid with a lower specific gravity. Then by removing the light solution, the photosynthetic microorganisms contained in the heavy solution are appropriately condensed. Preferably as large an amount of the light solution as possible is removed. The centrifugal separation process may be performed with a centrifugal acceleration between 5.000g and 20.000g. [0027] Both the supernatant liquid obtained after the sedimentation condensation and the light solution obtained after the centrifugal separation process may be put back into the culture solution to be used in the culturing step 9 S1 and mixed with the culture solution. Thus the culture solution is more efficiently used. In addition the photosynthetic microorganisms which are contained in the supernatant liquid and the light solution and removed without being condensed are cultured again. [00281 The photosynthetic microorganisms are kept at 25 to 40 degree centigrade with no light radiated on the photosynthetic microorganisms in the oil and fat conversion process S2. When the culture solution is kept under the conditioned above mentioned, carbohydrates stored in the cells are to be converted to oils and fats. [0029] The extraction process S3 to be subsequently performed is to extract oils and fats from inside the cells of the photosynthetic microorganisms. Oils and fats in the cells are to be extracted, for example, by a solvent extraction method with an organic solvent or a supercritical CO 2 extraction method. An organic solvent which may be used for the solvent extraction method is, for example, hexane. Since the hexane used for extracting oils and fats is reused for the solvent extraction after being distilled, this process has an advantage with respect to the environment and the production cost. [0030] Such oils and fats as triglyceride and ester compounds (wax ester) made of a long chain fatty acid and a higher alcohol with one or two hydroxyl groups are extracted in this process. [0031] It is preferable to perform a drying process S22 between the oil and fat conversion process S2 and the extraction process S3. In this drying process S22, the culture solution that contains the cultured photosynthetic microorganisms is dried. Since water which blocks the extraction is removed, oils and fats are more efficiently extracted after the drying process is performed. [0032] Although the culture solution may be dried in the drying process through 10 sun-dry, heated air dry or freeze drying method, it is preferable to make use of waste heat in exhaust gas or vapor discharged from factories or incineration plants. The culture solution is sufficiently dried without a need of other energies which work more rapidly and securely. [0033] It is preferable to perform a collection process S32 after the extraction process S3. In the collection process S32 the photosynthetic microorganisms out of which oils and fats have been extracted in the extraction process S3 and which are defatted. The defatted photosynthetic microorganisms contain proteins that constitute cells and pigment components and are utilized for biomass raw materials such as feed, fertilizer, solid fuel and raw materials for chemical products. [00341 A reforming process S4, in which the extracted oils and fats are modified, is subsequently performed. Reforming oils and fats corresponds to a reduction process such as a hydrogenation reaction. For example, a biofuel such as gas oil and jet fuel is produced by removing oxygen in wax esters through the hydrogenation reaction. [0035] Next, an embodiment of a biofuel production apparatus of the present invention is explained with reference to Fig.2. The biofuel production apparatus shown in Fig.2 corresponds to an example of a preferable embodiment of the production method for biofuel of the present invention. [00361 As shown in Fig.2, a biofuel production apparatus A includes a culturing means 1, a condensing means 12 to condense the culture solution through sedimentation condensation and centrifugal condensation, an oil and fat converting means 2, a drying means 22 and an extraction means 4. Each of these means corresponds to a process of the similar name. Therefore an explanation is skipped on significance, an act and an effect of each of these means which have been already explained in the description on the 11 corresponding process. [0037] The culturing means 1 may be, for example, a culture apparatus. The culture solution which is prepared for culturing photosynthetic microorganisms is put in the culture apparatus. In this embodiment, the culturing means 1 may be equipped with a carbon-dioxide gas supply means (not shown) to intentionally introduce into the culture apparatus carbon-dioxide gas which is a carbon source, a pH keeping means 14 to keep the pH of the culture solution between 2 and 6 in the acid region and a nutritious salt supply means (not shown) to supply nutritious salts to the culturing solution. [0038] The culturing process may be performed with the culture apparatus exposed to the atmosphere without any cover to cover an upper side of the culture apparatus. If the cover is attached over the culture apparatus, it is preferable to have a light transmission means (not shown) through which sun light or illumination light transmits. The photosynthesis reaction progresses with the illumination light being radiated. Making use of the light transmission means and/or an illumination device, it is possible to have the photosynthesis of the photosynthetic microorganisms progress. [0039] When a carbon-dioxide gas supply means is used, it is preferable to use an airtight culture apparatus in order not to have the carbon-dioxide gas leak. In addition, the culture apparatus may be equipped with a temperature sensor to measure a temperature of the culture solution or an inside of the culture apparatus, a light intensity sensor, a gas concentration sensor to measure a oxygen concentration or a carbon-dioxide concentration, a temperature control device to keep the temperature of the culture solution at a constant temperature and a stirring device to stir the culture solution (none of them shown). [0040] A condensation means 12 to perform the sedimentation condensation is, for example, a sedimentation tank. When the sedimentation tank is disposed 12 with an upper solution level in the sedimentation tank being higher than an upper solution level in the culture apparatus, the supernatant liquid in the sedimentation tank is transferred back into the culture apparatus due to a difference in the solution level between the sedimentation tank and the culture apparatus. The transferred supernatant liquid contains nutritious components and these nutritious components are reused and not wasted by transferring back the supernatant liquid. [00411 A condensation means for the centrifugal separation process may be, for example, a centrifugal machine of a disk type. The light solution obtained after the centrifugal separation process may be put back into the culture apparatus to reduce an amount of water to be used. On the other hand, the heavy solution which has been condensed through the centrifugal separation process is supplied to the oil and fat conversion means 2. [00421 An oil and fat conversion means 2 may be, for example, an airtight tank which is capable of blocking light coming therein. This airtight tank may be equipped with a temperature control device to keep the temperature of the condensed culture solution at a constant temperature, and a gas concentration sensor to measure a oxygen concentration or a carbon-dioxide concentration, and may be further equipped with a gas introduction means and a gas discharge means which are constituted by one way valves and other components if oxygen is purged from the airtight tank by having an inert gas such as nitrogen or argon flow in the airtight tank to keep the airtight tank under the anaerobic condition. [0043] A drying means 22 may be, for example, a drying machine making use of waste heat in waste gas or waste vapor discharged from factories or incineration plants. The drying machine for the present invention is not limited to this type and may be a heated air drying machine that is commercially available or a freeze dry drying machine. In addition, the drying process S22 may be performed through sun-dry as has been explained. Water included in the 13 culture solution is evaporated to water vapor and removed. [0044] The extraction means 3 may be, for example, a solvent extraction apparatus to extract oils and fats with an organic solvent or a supercritical CO 2 extraction which extracts oils and fats with CO 2 in a supercritical condition. Organic solvents such as hexane may be used for this extraction process, as has been mentioned. As has been explained for the extraction process 3, the defatted microorganisms from which oils and fats have been extracted are collected and used for such raw biomass materials as feedstuff, fertilizer, solid fuel, and raw materials for chemical products after organic components of the defatted microorganism are biologically or chemically dissolved or dried. [0045] A reforming means may be, for example, such a hydrodesulfurization unit as a hydrodesulfurization unit for kerosene and gas oil, a hydrodesulfurization unit for vacuum gas oil, a naphtha hydrorefining unit and a hydrodesulfurization unit for heavy oil, which perform hydrogenation reactions. Among these units, the hydrodesulfurization unit for kerosene and gas oil is capable of efficiently producing such a biofuel as gas oil and jet fuel. [0046] EXAMPLE Hereinafter is explained a production method for biofuel of the present invention specifically, with reference to an example. [0047] This example utilizes Euglena as photosynthetic microorganisms. Firstly, a modified Cramer-Myers culture medium (including (NH 4
)
2 HPO4 1.0g/L, KH 2
PO
4 1.0g/L, MgSO 4 -7H 2 0 0.2g/L, CaCl 2 -2H 2 0 0.02g/L, Fe 2 (SO2) 3 -7H 2 0 3 mg/L, MnCl2-4H 2 0 1.8 mg/L, CoSO 4 -7H20 1.5 mg/L, ZnSO 4 -7H 2 0 0.4 mg/L, Na 2 MoO 4 -2H20 0.2 mg/L, CuS0 4 -5H 2 0 0.02 g/L, thiamin chloride(vitamin B 1 ) 0.1 mg/L, and cyanocobalamin(vitaminB12) and with pH3.5) was prepared by adding nutritious salts such as a nitrogen source, a phosphor 14 source and minerals into water stored in a culture apparatus. [0048] Euglena was inoculated in the prepared culture solution and cultured for seven days with sun light radiated on the Euglena. A temperature of the culture solution was kept within a range of 29 'C±3 "C during the culture period. Carbon-dioxide gas was introduced in the culture apparatus during the culture period. The pH of the culture solution became lower for an acid with the carbon dioxide gas being introduced and was kept in a acidic range between 2 and 6 with a pH keeping apparatus. When Euglena was cultured in the culture media whose pH is approximately between 2 and 4.5, it was difficult for other microorganisms to breed and contamination was effectively prevented. [0049] The liquid depth of the culture solution was kept less than or equal to 50 cm. However when the liquid depth of the culture solution was kept less than or equal to 30 cm, photosynthetic reaction progressed more efficiently because the culture solution in the lower portion was efficiently replaced with the culture solution in the upper portion by stirring the culture solution. A predetermined amount of the culture solution in the culture apparatus was taken out of the culture apparatus and transferred into a sedimentation tank. Since the Euglena had a larger specific gravity than that of water, the Euglena settled on the bottom of the sedimentation tank through a natural sedimentation process in which the Euglena was coming down toward the bottom of the sedimentation tank. A predetermined amount of the culture solution inclusive of the settled Euglena was transferred into a centrifugal machine. The supernatant liquid was transferred back into the culture apparatus after centrifugal separation process and used again for culturing Euglena. [0050] In the centrifugal separation process, the condensed solution which included the Euglena and transferred into a centrifugal machine was centrifuged. Then a heavy solution which included the Euglena further condensed and had a higher concentration the Euglena was obtained. Subsequently this condensed 15 heavy solution was sent to the oil and fat conversion process. A light solution including the Euglena which was not separated into the heavy solution was transferred into the culture apparatus and used again for culturing Euglena. [00511 In the oil and fat conversion process, the heavy solution was kept in the anaerobic atmosphere to accelerate metabolism in the cultured Euglena and increase their content rate of oils and fats. In this process, the heavy solution was kept between 25 *C and 40 'C under the anaerobic condition with no sun light being radiated on the heavy solution. When the oil and fat conversion process was performed on the culture solution which was not condensed in the condensing process, an amount of the culture solution, in which oxygen discharged by the Euglena through the photosynthetic reaction was dissolved, is so large that it took a longer time to decrease the concentration of oxygen dissolved in the culture solution and suppress respiration of the cultured Euglena. Therefore a larger amount of an inert gas had to be used to create the anaerobic condition. On the other hand, when the oil and fat conversion process was performed on the cultured solution that was condensed in the condensing process, the amount of the culture solution, in which a concentration of the Euglena was higher and oxygen discharged by the Euglena through photosynthetic reaction was dissolved, was not so large and it did take a shorter time to decrease the concentration of oxygen dissolved in the culture solution. As a result, the amount of the inert gas to create the anaerobic condition became smaller. [0052] Next, the Euglena, whose content rate of oils and fats was increased by the anaerobic process, was dried at 110 *C on the waste heat for 120 minutes. Oils and fats were extracted out of the dried stuff in the dried Euglena through a solvent extraction method with hexane and the dried Euglena was divided in oils and fats and the defatted dried stuff of the Euglena. By purifying oils and fats through a hydrodesulfurization unit for kerosene and gas oil, petroleum substitute fuel (biofuel) was produced. This 16 petroleum substitute fuel is presumed to be capable of being used for gas oil and jet fuel because it consists mainly of oils and fats of carbon compounds whose carbon length is fourteen. In addition, the defatted Euglena was collected and utilized for raw biomass materials to be used for feedstuff and fertilizer. [00531 Description of Signs S1 Culturing process S12 Condensing process S2 Oil and fat conversion process S22 Drying process S3 Extraction process S32 Collection process S4 Reforming process A Biofuel production apparatus 1 Culturing means 12 Condensing means 14 pH keeping means 2 Oil and fat converting means 22 Drying means 3 Extracting means 4 Reforming means 17
Claims (12)
1. A production method for biofuel comprising; a culturing process of culturing in a culture solution photosynthetic microorganisms which store oils, fats and carbohydrates in cells of the photosynthetic microorganisms; an oil and fat conversion process of converting the carbohydrates stored in the cells of the photosynthetic microorganisms cultured in the culture apparatus to oils and fats; an extraction process of extracting the oils and fats out of the cells of the photosynthetic microorganisms, and a reforming process to reform the extracted oils and fats.
2. The production method for biofuel as described in claim 1 further comprising a condensing process of condensing the culture solution in which the photosynthetic microorganisms are cultured, the condensing process being performed between the culturing process and the extraction process.
3. The production method for biofuel as described in claim 2 further wherein condensing the culture solution is performed through sedimentation condensation in the condensing process.
4. The production method for biofuel as described in claim 2 further wherein the culture solution is condensed through centrifugal separation following the sedimentation condensation.
5. The production method for biofuel as described in claim 1 further comprising a drying process of drying the culture solution inclusive of the cultured photosynthetic microorganisms between the oil and fat conversion process and the extraction process. 18
6. The production method for biofuel as described in claim 1 further comprising a collection process of collecting the defatted photosynthetic microorganisms out of which oils and fats are extracted after the extraction process.
7. The production method for biofuel as described in claim 3 wherein putting a supernatant liquid obtained through the sedimentation condensation back into the culture apparatus used in the culturing process.
8. The production method for biofuel as described in claim 4 wherein putting a supernatant liquid obtained through the centrifugal separation back into the culture apparatus used in the culturing process.
9. The production method for biofuel as described in claim 1 wherein culturing the photosynthetic microorganisms is performed in the culture solution with a carbon-dioxide concentration that is higher than one in the culture solution in which air is introduced.
10. The production method for biofuel as described in claim 1 wherein a liquid depth of the culture solution in the culturing process is less than or equal to 50 cm.
11. The production method for biofuel as described in claim 1 wherein a pH of the culture solution in the culturing process is kept between 2 and 6.
12. The production method for biofuel as described in claim 1 wherein in the oil and fat conversion process the culture solution is kept between 25 0 C and 40 'C and no light is radiated on the culture solution. 19
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MX2014004605A (en) * | 2011-10-28 | 2014-05-27 | Sapphire Energy Inc | Processes for upgrading algae oils and products thereof. |
JP5833634B2 (en) * | 2012-01-31 | 2015-12-16 | 株式会社ユーグレナ | Method for producing fuel oil base material |
JP6060441B2 (en) * | 2012-07-06 | 2017-01-18 | 株式会社ユーグレナ | Algae-containing composition production system |
US9024096B2 (en) * | 2012-12-11 | 2015-05-05 | Lummus Technology Inc. | Conversion of triacylglycerides-containing oils |
JP6183825B2 (en) * | 2013-03-04 | 2017-08-23 | 国立大学法人 東京大学 | Method and apparatus for concentrating microalgae culture solution |
JP6063362B2 (en) * | 2013-09-10 | 2017-01-18 | 株式会社日立製作所 | Photosynthesis microorganism separation system and separation method thereof |
CN104630065B (en) * | 2013-11-14 | 2018-08-28 | 中国石油化工股份有限公司 | A kind of integrated processes of both culturing microalgae and exhaust gas denitration |
JP6290639B2 (en) * | 2014-01-31 | 2018-03-07 | 株式会社ユーグレナ | Euglena culture method and culture solution thereof |
JP2016002004A (en) * | 2014-06-13 | 2016-01-12 | 株式会社デンソー | Method for culturing microalgae, microalgae and method for producing fat |
CN104445800B (en) * | 2014-10-21 | 2016-06-01 | 南通大学 | Utilize micro-algae coupling light bioreactor sewage treatment and the method producing diesel oil |
JP2016182063A (en) * | 2015-03-26 | 2016-10-20 | 国立大学法人 東京大学 | Culture medium, and method for suppressing strong light impediment |
JP6031158B1 (en) * | 2015-06-10 | 2016-11-24 | 株式会社ユーグレナ | Euglena culture method |
JP6740590B2 (en) * | 2015-10-23 | 2020-08-19 | 株式会社デンソー | Photosynthetic microorganism culturing apparatus and culturing method |
US9901606B2 (en) | 2016-06-09 | 2018-02-27 | Algaeon, Inc. | Euglena lysate composition |
EP3494093B1 (en) | 2016-08-19 | 2022-05-18 | Noblegen Inc. | Methods and uses of dissolved organic material fractions for binding metal ions |
CA3059271A1 (en) | 2017-04-07 | 2018-10-11 | Noblegen Inc. | Methods and uses of encapsulated exudates and dried euglena biomass for binding metal |
KR102612831B1 (en) * | 2021-06-07 | 2023-12-13 | 한국지역난방공사 | Hybrid system of CO2 compact membrane separation and utilization for urban power plants for Effluent CO2 concetration control |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59118090A (en) * | 1982-12-22 | 1984-07-07 | Shozaburo Kitaoka | Preparation of wax ester, higher fatty alcohol and higher fatty acid |
JPS61254193A (en) * | 1985-05-07 | 1986-11-11 | Harima Chem Inc | Production of unssaturated wax ester |
JPS63119409A (en) * | 1986-11-08 | 1988-05-24 | Harima Chem Inc | Skin cosmetic |
JP3004509B2 (en) * | 1993-09-27 | 2000-01-31 | 三菱重工業株式会社 | Method and apparatus for producing ethanol from microalgae |
JP3468955B2 (en) * | 1995-12-01 | 2003-11-25 | 三菱重工業株式会社 | Method for producing lactic acid by microalgae |
JP3549444B2 (en) * | 1998-07-28 | 2004-08-04 | 喜温 三浦 | Method for producing hydrogen by microorganisms |
US9637714B2 (en) | 2006-12-28 | 2017-05-02 | Colorado State University Research Foundation | Diffuse light extended surface area water-supported photobioreactor |
MX2010006539A (en) * | 2007-12-11 | 2011-02-23 | Synthetic Genomics Inc | Secretion of fatty aicds by photosynthetic microorganisms. |
JP2010057485A (en) * | 2008-08-08 | 2010-03-18 | Mitsubishi Chemicals Corp | Method for immobilizing carbon dioxide, and alga-culturing apparatus for immobilizing carbon dioxide |
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WO2011148981A1 (en) | 2011-12-01 |
JP2011246605A (en) | 2011-12-08 |
CN103003433A (en) | 2013-03-27 |
KR101462411B1 (en) | 2014-11-17 |
MY159579A (en) | 2017-01-13 |
BR112012029999A2 (en) | 2015-09-08 |
AU2011259331B2 (en) | 2013-12-05 |
US20130071889A1 (en) | 2013-03-21 |
EP2578689A1 (en) | 2013-04-10 |
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