AU2006249244B2 - 84P2A9: A prostate and testis specific protein highly expressed in prostate cancer - Google Patents

Description

AUSTRALIA

Patents Act 1990 AGENSYS, INC.

COMPLETE SPECIFICATION STANDARD PATENT Invention Title: 84P2A 9: A prostate and testis specific protein highly expressed in prostate cancer The following statement is a full description of this invention including the best method of performing it known to us:-

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84P2A9: A PROSTATE AND TESTIS SPECIFIC PROTEIN HIGHLY (-i EXPRESSED IN PROSTATE CANCER This application is a divisional application from Australian Patent Application No. 2001237973, the entire contents of which are incorporated herein by reference.

FIELD OF THE INVENTION C The invention described herein relates to a novel gene and its encoded protein, termed 84P2A9, and to diagnostic and therapeutic methods and compositions useful in the management of various cancers that express 84P2A9, particularly prostate cancers.

BACKGROUND OF THE INVENTION Cancer is the second leading cause of human death next to coronary disease.

Worldwide, millions of people die from cancer every year. In the United States alone, cancer causes the death of well over a half-million people annually, with some 1.4 million new cases diagnosed per year. While deaths from heart disease have been declining significantly, those resulting from cancer generally are on the rise. In the early part of the next century, cancer is predicted to become the leading cause of death.

Worldwide, several cancers stand out as the leading killers. In particular, carcinomas of the lung, prostate, breast, colon, pancreas, and ovary represent the primary causes of cancer death. These and virtually all other carcinomas share a common lethal feature. With very few exceptions, metastatic disease from a carcinoma is fatal. Moreover, even for those cancer patients who initially survive their primary cancers, common experience has shown that their lives are dramatically altered. Many cancer patients experience strong anxieties driven by the awareness of the potential for recurrence or treatment failure. Many cancer patients experience physical debilitations following treatment. Furthermore, many cancer patients experience a recurrence.

Worldwide, prostate cancer is the fourth most prevalent cancer in men. In North America and Northern Europe, it is by far the most common cancer in males and is the second leading cause of cancer death in men. In the United States alone, well over 40,000 men die annually of this disease second only to lung cancer. Despite the 1A ND magnitude of these figures, there is still no effective treatment for metastatic prostate Scancer. Surgical prostatectomy, radiation therapy, hormone ablation therapy, surgical 0 castration and chemotherapy continue to be the main treatment modalities.

SUnfortunately, these treatments are ineffective for many and are often associated with undesirable consequences.

On the diagnostic front, the lack of a prostate tumor marker that can accurately detect early-stage, localzed tumors remains a significant limitation in the diagnosis and management of this disease. Although the serum prostate specific antigen (PSA) assay Shas been a very useful tool, however its specificity and general utility is widely regarded C 10 as lacking in several important respects.

0 Progress in identifying additional specific markers for prostate cancer has been C, improved by the generation of prostate cancer xenografts that can recapitulate different stages of the disease in mice. The LAPC (Los Angeles Prostate Cancer) xenografts arc prostate cancer xenografts that have survived passage in severe combined immune deficient (SCID) mice and have exhibited the capacity to mimic the transition from androgen dependence to androgen independence (Klein et al., 1997, Nat. Med.3:402).

More recently identified prostate cancer markers include PCTA-1 (Su et al., 1996, Proc.

Natl. Acad. Sci. USA 93: 7252), prostate-specific membrane (PSM) antigen (Pinto et al., Clin Cancer Res 1996 Sep;2( 9 ):1 4 4 5-5 1 STEAP (Proc Natl Acad Sci U S A. 1999 Dec 7;96(25):14523-8) and prostate stem cell antigen (PSCA) (Reiter et al., 1998, Proc. Natl.

Acad. Sci. USA 95: 1735).

While previously identified markers such as PSA, PSM, PCTA and PSCA have facilitated efforts to diagnose and treat prostate cancer, there is need for the identification of additional markers and therapeutic targets for prostate and related cancers in order to further improve diagnosis and therapy.

SUMMARY OF THE INVENTION The present invention relates to a novel, largely prostate and testis-related gene, designated 84P2A9, that is over-expressed in multiple cancers including prostate, testis, kidney, brain, bone, skin, ovarian, breast, pancreas, colon, lymphocytic and lung cancers.

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SNorthern blot expression analysis of 84P2A9 gene expression in normal tissues shows a Shighly prostate and testis-related expression pattern in adult tissues. Analysis of d 84P2A9 expression in normal prostate and prostate tumor xenografts shows overexpression in LAPC-4 and LAPC-9 prostate tumor xenografts, with the highest expression in LAPC-9. The nucleotide (SEQ ID NO: 1) and amino acid (SEQ ID NO: 2) sequences of 84P2A9 are shown in FIG. 2. Portions of the 84P2A9 amino acid sequence show some homologies to ESTs in the dbEST database. The prostate and Cl testis-related expression profile of 84P2A9 in normal adult tissues, combined with the over-expression observed in prostate tumor xenografts, shows that 84P2A9 is O 10 aberrantly over-expressed in at least some cancers, and thus serves as a useful diagnostic and/or therapeutic target for cancers such as prostate, testis, kidney, brain, cK bone, skin, ovarian, breast, pancreas, colon, lymphocytic and lung cancers (see, e.g., FIGS. 4-8).

The invention provides polynucleotides corresponding or complementary to all or part of the 84P2A9 genes, mRNAs, and/or coding sequences, preferably in isolated form, including polynucleotides encoding 84P2A9 proteins and fragments of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or more amino acids, DNA, RNA, DNA/RNA hybrids, and related molecules, polynucleotides or oligonucleotides complementary or having at least a 90% homology to the 84P2A9 genes or mRNA sequences or parts thereof, and polynucleotides or oligonucleotides that hybridize to the 84P2A9 genes, mRNAs, or to 84P2A9-encoding polynucleotides. Also provided are means for isolating cDNAs and the genes encoding 84P2A9. Recombinant DNA molecules containing 84P2A9 polynucleotides, cells transformed or transduced with such molecules, and host-vector systems for the expression of 84P2A9 gene products are also provided. The invention further provides antibodies that bind to 84P2A9 proteins and polypeptide fragments thereof, including polyclonal and monoclonal antibodies, murine and other mammalian antibodies, chimeric antibodies, humanized and fully human antibodies, and antibodies labeled with a detectable marker.

The present invention further provides an isolated polynucleotide that encodes a 84P2A9 protein, wherein the polynucleotide is selected from the group consisting of: a polynucleotide comprising the sequence of SEQ ID NO: 1, from nucleotide residue number 165 through nucleotide residue number 1676; a polynucleotide comprising the sequence of SEQ ID NO: 1, from nucleotide residue number 165 through nucleotide residue number 1676, wherein T can also be U; and

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a polynucleotide encoding a 84P2A9 protein that is at least 90% identical <d to the amino acid sequence shown in SEQ ID NO: 2 over its entire length.

The present invention further provides a polynucleotide that is fully C complementary to a polynucleotide according to the invention.

The present invention further provides a recombinant expression vector comprising a polynucleotide according to the invention.

The present invention further provides a recombinant viral expression vector that comprises a polynucleotide that encodes an 84P2A9 protein, wherein the polynucleotide is: 0 10 a polynucleotide that encodes the amino acid sequence of SEQ ID NO:2; or C a polynucleotide that encodes a sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO:2; or a polynucleotide comprising SEQ ID NO:1, wherein T can also be U; or a polynucleotide comprising the sequence of SEQ ID NO:1, from nucleotide residue number 165 through nucleotide residue number 1676, wherein T can also be U.

The present invention further provides a host cell that contains an expression vector according to the invention.

The present invention further provides a process for producing a 84P2A9 protein encoded by the polynucleotide according to the invention comprising culturing a host cell of the invention under conditions sufficient for the production of the 84P2A9 protein.

The present invention further provides a 84P2A9 protein produced by the process of the invention.

The present invention further provides an isolated 84P2A9 protein, wherein the 84P2A9 protein is encoded by the polynucleotide according to the invention.

The present invention further provides an isolated peptide selected from the group consisting of KKRK, NQTN, NCSV, TNK, SRR, SSK, SVR, GLFTND, GGACGI, GGTPTS, GTPTSM and GSLCTG, wherein the isolated peptide is derived from SEQ ID NO: 2.

The present invention further provides an antibody or fragment thereof that immunospecifically binds to an epitope on a 84P2A9 protein wherein the protein is encoded by a polynucleotide according to the invention.

The present invention further provides a hybridoma that produces an antibody according to the invention.

The present invention further provides a vector comprising a polynucleotide

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"encoding a single chain monoclonal antibody according to the invention.

d) The invention further provides methods for detecting the presence and status of S84P2A9 polynucleotides and proteins in various biological samples, as well as methods for identifying cells that express 84P2A9. A typical embodiment of this invention provides methods for monitoring 84P2A9 gene products in a tissue or haematology sample having or suspected of having some form of growth disregulation such as Scancer.

The present invention further provides a method for detecting the presence of a C84P2A9 protein encoded by the polynucleotide according to the invention or a polynucleotide of the invention in a test sample comprising: C( contacting the sample with an antibody or polynucleotide, respectively, that specifically binds to the 84P2A9 protein or polynucleotide, respectively; and detecting binding of 84P2A9 protein or polynucleotide, respectively, in the sample thereto.

The invention further provides various immunogenic or therapeutic compositions and strategies for treating cancers that express 84P2A9 such as prostate cancers, including therapies aimed at inhibiting the transcription, translation, processing or function of 84P2A9 as well as cancer vaccines.

The present invention further provides a pharmaceutical composition comprising a pharmaceutically acceptable carrier and the recombinant expression vector of the invention.

The present invention further provides a method of inhibiting growth of a cell expressing a 84P2A9 protein encoded by the polynucleotide according to the invention, comprising providing an effective amount of an antibody according to the invention to the cell, whereby the growth of the cell is inhibited.

The present invention further provides a method of delivering a cytotoxic agent to a cell expressing a 84P2A9 protein encoded by the polynucleotide according to the invention, comprising providing an effective amount of an antibody of the invention to the cell.

The present invention further provides a method of inducing an immune response to a 84P2A9 protein encoded by the polynucleotide according to the invention, said method comprising: providing a 84P2A9 protein epitope; contacting the epitope with an immune system T cell or B cell, whereby the immune system T cell or S1B cell is induced.

The present invention further provides a method of generating an immune response in a subject comprising administering to the subject an immunogenic amount of the viral vector of the invention.

The present invention further provides use of the viral vector of the invention in the manufacture of a medicament for generating an immune response in a subject.

SThe present invention further provides use of an antibody according to the invention in the manufacture of a medicament for inhibiting growth of a cell expressing a 84P2A9 protein encoded by the polynucleotide according to the invention.

Throughout this specification the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element, integer or step, or group of elements, integers or steps, but not the exclusion of any other element, integer or step, or group of elements, integers or steps.

Any discussion of documents, acts, materials, devices, articles or the like which has been included in the present specification is solely for the purpose of providing a context for the present invention. It is not to be taken as an admission that any or all of these matters form part of the prior art base or were common general knowledge in the field relevant to the present invention as it existed before the priority date of each claim of this application.

BRIEF DESCRIPTION OF THE FIGURES FIG. 1. shows the 84P2A9 suppression subtractive hybridization (SSH) DNA sequence of about 425 nucleotides in length (SEQ ID NO: This sequence was identified in comparisons of cDNAs from various androgen dependent and androgen independent LAPC xenografts.

FIG. 2. shows the nucleotide (SEQ ID NO: 1) and amino acid (SEQ ID NO: 2) sequences of 84P2A9. See Example 2, infra. The sequence surrounding the start ATG (AAC ATG G) (SEQ ID NO: 4) exhibits a Kozak sequence (A at position-3, and G at position The start methionine with Kozak sequence is indicated in bold, the nuclear localisation signals are boxed.

FIGS. 3A and 3B. show the amino acid sequence alignment of 84P2A9 (SEQ ID NO:2) with KIAA1552 (SEQ ID NO: 5) and LUCA15 (SEQ ID NO: FIG. 3A shows that the 84P2A9 protein sequence (bottom line) has some homology to the human brain protein KIAA 1152 (39.5% identity a 337 amino acid region, Score: 407.0; Gap frequency: FIG. 3B shows that the 84P2A9 protein sequence (bottom line)

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Scontains a domain that is homologous to a portion of the LUCA15 tumor suppressor d) protein (64.3% identity over a 42 amino acid region, Score: 138.0; Gap frequency: S0.0%).

FIGS. 4A-4C. show the Northern blot analysis of the restricted 84P2A9 expression in various normal human tissues (using the 84P2A9 SSH fragment as a probe) and LAPC xenografts. Two multiple tissue northern blots (Clontech) (FIGS. 4A and 4B) Sand a xenograft northern blot (FIG. 4C) were probed with the 84P2A9 SSH fragment.

O Lanes 1-8 in FIG. 4A consist of mRNA from heart, brain, placenta, lung, liver, skeletal Smuscle, kidney and pancreas respectively. Lanes 1-8 in FIG. 4B consist of total RNA from spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes respectively. Lanes 1-5 in FIG. 4C consist of mRNA from prostate, LAPC-4 AD, LAPC-4 AI, LAPC-9 AD and LAPC-9 AI respectively. Size standards in kilobases (kb) are indicated on the side. Each lane contains 2 pg of mRNA. for the normal tissues and pLg of total RNA for the xenograft tissues. The results show the expression of M\ 10 84P2A9 in testis and prostate and the LAPC xenografts.

FIG. 5. shows the Northern blot analysis of 84P2A9 expression in prostate and multiple cancer cell lines. Lanes 1-56 show expression in LAPC-4 AD, LAPC-4 Al, LAPC-9 AD, LAPC-9 AI, LNCaP, PC-3, DU145, TsuPrl, LAPC-4 CL, HT1197, SCaBER, UM-UC-3, TCCSUP, J82, 5637, 293T, RD-ES, PANC-1, BxPC-3, HPAC, Capan-1, SK-CO-1, CaCo-2, LoVo, T84, Colo-205, KCL 22, PFSK-1, T98G, SK-ES-1, HOS, U2-OS, RD-ES, CALU-1, A427, NCI-H82, NCI-H146, 769-P, A498, CAKI-1, SW839, BT20, CAMA-1, DU4475, MCF-7, MDA-MB-435s, NTERRA-2, NCCIT, TERA-1, TERA-2, A431, HeLa, OV-1063, PA-1, SW626 and CAOV-3 respectively.

High levels of 84P2A9 expression were detected in brain (PFSK-1, T98G), bone (HOS, U2-OS), lung (CALU-1, NCI-H82, NCI-H146), and kidney (769-P, A498, CAKI-1, SW839) cancer cell lines. Moderate expression levels were detected in several pancreatic (PANC-1, BxPC-3, HPAC, CAPAN-1), colon (SK-CO-1, CACO-2, LOVO, COLO- 205), bone (SK-ES-1, RD-ES), breast (MCF-7, MDA-MB-435s) and testicular cancer (NCCIl) cell lines.

FIG. 6. shows the Northern blot analysis of 84P2A9 expression in prostate cancer patient samples. Prostate cancer patient samples show expression of 84P2A9 in both the normal and the tumor part of the prostate tissues. Lanes 1-7 show Normal prostate, Patient 1 normal adjacent tissue, Patient 1 Gleason 9 tumor, Patient 2 normal adjacent tissue, Patient 2 Gleason 7 tumor and Patient 3 Gleason 7 tumor respectively.

N These results provide evidence that 84P2A9 is a very testis specific gene that is upregulated in prostate cancer and potentially other cancers. Similar to the MAGE 0 antigens, 84P2A9 may thus qualify as a cancer-testis antigen (Van den Eynde and Boon, SIntJ Clin Lab Res. 27:81-86, 1997).

FIG. 7. shows RNA was isolated from kidney cancers and their adjacent normal tissues obtained from kidney cancer patients. Lanes 1-15 show 769-P- clear cell type; A498 clear cell type; SW839 clear cell type; Normal Kidney; Patient 1, N; Patient 1, tumor; Patient 2, N; Patient 2, tumor, clear cell type, grade III; Patient 3, N; C 10 Patient 3. tumor, clear cell type, grade II/IV; Patient 4, N; Patient 4, tumor, clear cell

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O type, grade II/IV; Patient 5, N; Patient 5, tumor, clear cell type, grade II; and Patient 6, C' tumor, metastasis to chest wall respectively (N=normal adjacent tissue and CL=.cell line).

Northern analysis was performed using 10pg of total RNA for each sample. Expression of 84P2A9 was seen in all 6 tumor samples tested as well as in the three kidney cell lines, 769-P, A498 and SW839.

FIG. 8. shows RNA was isolated from colon cancers and their adjacent normal tissues obtained from colon cancer patients. Lanes 1-11 show Colo 205; LoVo; T84; Caco-2; Patient 1, N; Patient 1, tumor, grade 2, T3N1Mx (positive for lymph node metastasis); Patient 2, N; Patient 2, tumor, grade 1, T2NOMx; Patient 3, N; Patient 3, tumor, grade 1, T2NMx (positive for lymph node metastasis); and Patient 4, tumor, grade 2, T3 N1 MX (positive for lymph node metastasis); respectively (N=normal adjacent tissue and CL=cell line). Northern analysis was performed using 10g of total RNA for each sample. Expression of 84P2A9 was seen in all 4 tumor samples tested as well as in the 4 colon cancer cell lines Colo 205, LoVo, T84 and Caco-2.

FIG. 9. Shows expression of 84P2A9 assayed in a panel of human cancers (T) and their respective matched normal tissues on RNA dot blots. Cancer cell lines from left to right are HeLa (cervical carcinoma), Daudi (Burkitt's lymphoma), K562 (CML), HL-60 (PML), G361 (melanoma), A549 (lung carcinoma), MOLT-4 (lymphoblastic leuk.), SW480 (colorectal carcinoma) and Raji (Burkitt's lymphoma).

D 84P2A9 expression was seen in kidney cancers, breast cancers, prostate cancers, lung cancers, stomach cancers, colon cancers, cervical cancers and rectum cancers. 84P2A9 O was also found to be highly expressed in a panel of cancer cell lines, specially the MOLT- C 4 lymphoblastic leukemia and the A549 lung carcinoma cell lines. The expression detected in normal adjacent tissues (isolated from diseased tissues) but not in normal tissues, isolated from healthy donors, can indicate that these tissues are not fully normal and that 84P2A9 can be expressed in early stage tumors.

FIG. 10 shows the expression of 84P2A9 in bladder cancer patient specimens.

Expression of 84P2A9 was seen in 4 bladder cancer patient specimens tested and in three bladder cell lines UM-UC-3 (lane 1),J82 (lane 2) and SCABER (lane RNA 0 was isolated from normal bladder bladder tumors and their adjacent normal C tissues obtained from 6 bladder cancer patients Tumor from P1 is transitional carcinoma, grade 4; P2 is invasive squamous carcinoma; P3 is transitional carcinoma, grade 3; P4 is non-invasive papillary carcinoma, grade 1/3; P5 is papillary carcinoma, grade 3/3; and P6 is transitional carcinoma, grade 3/2. Northern analysis was performed using 10pg of total RNA for each sample.

FIG. 11 shows the expression of 84P2A9 protein in 293T cells. 293T cells were transiently transfected with either pCDNA3.1 V5-HIS epitope tagged 84P2A9 plasmid or with empty control vector and harvested 2 days later. Cells were lysed in SDS-PAGE sample buffer and lysates were separated on a 10-20% SDS-PAGE gel and then transferred to nitrocellulose. The blot was blocked in Tris-buffered saline (TBS)+ 2 non-fat milk and then probed with a 1:3,000 dilution of murine anti-V5 monoclonal Ab (Invitrogen) in TBS+0.15% Tween-20+ 1% milk. The blot was washed and then incubated with a 1:4,000 dilution of anti-mouse IgG-HRP conjugate secondary antibody.

Following washing, anti-V5 epitope immunoreactive bands were developed by enhanced chemiluminescence and visualized by exposure to autoradiographic film. Indicated by arrow is a specific anti-V5 immunoreactive band of approximately 87 Kd that corresponds to expression of the epitope-tagged 84P2A9 protein in the transfected cells.

I DETAILED DESCRIPTION OF THE INVENTION CUnless otherwise defined, all terms of art, notations and other scientific terms or terminology used herein are intended to have the meanings commonly understood by Sthose of skill in the art to which this invention pertains, In some cases, terms with commonly understood meanings are defined herein for clarity and/or for ready reference, and the inclusion of such definitions herein should not necessarily be construed to represent a substanual difference over what is generally understood in the C1 art. Many of the techniques and procedures described or referenced herein are generally 0wel understood and commonly employed using conventional methodology by those DO 10 skilled in the art, such as, for example, the widely utilized molecular cloning Smethodologies described in Sambrook et al., Molecular Cloning: A Laboratory Manual 2nd. edition (1989) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. As appropriate, procedures involving the use of commercially available kits and reagents are generally carried out in accordance with manufacturer defined protocols and/or parameters unless otherwise noted.

DEFINITIONS:

As used herein, the terms "advanced prostate cancer", "locally advanced prostate cancer", "advanced disease" and "locally advanced disease" mean prostate cancers that have extended through the prostate capsule, and are meant to include stage C disease under the American Urological Association (AUA) system, stage C1 C2 disease under the Whitmore-Jewett system, and stage T3 T4 and N+ disease under the TNM (tumor, node, metastasis) system. In general, surgery is not recommended for patients with locally advanced disease, and these patients have substantially less favorable outcomes compared to patients having clinically localized (organ-confined) prostate cancer.

Locally advanced disease is clinically identified by palpable evidence of induration beyond the lateral border of the prostate, or asymmetry or induration above the prostate base. Locally advanced prostate cancer is presently diagnosed pathologically following radical prostatectomy if the tumor invades or penetrates the prostatic capsule, extends into the surgical margin, or invades the seminal vesicles.

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O The term "antibody" is used in the broadest sense. Therefore an "antibody" can be C1 naturally occurring or man made such as monoclonal antibodies produced by conventional hybridoma technology. Anti-84P2A9 antibodies comprise monoclonal and polyclonal antibodies as well as fragments containing the antigen binding domain and/or one or more complementarity determining regions of these antibodies. As used herein, an antibody fragment is defined as at least a portion of the variable region of the immunoglobulin molecule that binds to its target, the antigen binding region. In one embodiment it C specifically covers single anti-84P2A9 antibody (including agonist, antagonist and H neutralizing antibodies) and anti-84P2A9 antibody compositions with polyepitopic \O 10 specificity. The term "monoclonal antibody" as used herein refers to an antibody obtained from a population of substantially homogeneous antibodies, the antibodies comprising the population are identical except for possible naturally-occurring mutations that are present in minor amounts.

The term "cytotoxic agent" as used herein refers to a substance that inhibits or prevents the function of cells and/or causes destruction of cells. The term is intended to include radioactive isotopes At 21 I11, I125, Y 90 Re' 86 Re18 8 Sm 153 Bi 212

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32 and radioactive isotopes of Lu), chemotherapeutic agents, and toxins such as small molecule toxins or enzymatically active toxins of bacterial, fungal, plant or animal origin, including fragments and/or variants thereof.

As used herein, the terms "hybridize", "hybridizing", "hybridizes" and the like, used in the context of polynucleotides, are meant to refer to conventional hybridization conditions, preferably such as hybridization in 50% formamide/6XSSC/0.1% SDS/100 pg/ml ssDNA, in which temperatures for hybridization are above 37 degrees C and temperatures for washing in 0.1XSSC/0.1% SDS are above 55 degrees C.

As used herein, a polynucleotide is said to be "isolated" when it is substantially separated from contaminant polynucleotides that correspond or are complementary to genes other than the 84P2A9 gene or that encode polypeptides other than 84P2A9 gene product or fragments thereof. A skilled artisan can readily employ nucleic acid isolation procedures to obtain an isolated 84P2A9 polynucleotide.

As used herein, a protein is said to be "isolated" when physical, mechanical or chemical methods are employed to remove the 84P2A9 protein from cellular constituents that are normally associated with the protein. A skilled artisan can readily employ standard purification methods to obtain an isolated 84P2A9 protein.

O The term "mammal" as used herein refers to any mammal classified as a mammal, Q including mice, rats, rabbits, dogs, cats, cows, horses and humans. In one preferred embodiment of the invention, the mammal is a mouse. In another preferred embodiment of the invention, the mammal is a human.

As used herein, the terms "metastatic prostate cancer" and "metastatic disease" mean prostate cancers that have spread to regional lymph nodes or to distant sites, and are meant to include stage D disease under the AUA system and stage TxNxM+ under 10 the TNM system. As is the case with locally advanced prostate cancer, surgery is

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Sgenerally not indicated for patients with metastatic disease, and hormonal (androgen Cl ablation) therapy is a preferred treatment modality. Patients with metastatc prostate cancer eventually develop an androgen-refractory state within 12 to 18 months of treatment initiation, and approximately half of these patients die within 6 months after developing androgen refractory status. The most common site for prostate cancer metastasis is bone. Prostate cancer bone metastases are often characteristically osteoblastic rather than osteolytic resulting in net bone formation). Bone metastases are found most frequently in the spine, followed by the femur, pelvis, rib cage, skull and humerus. Other common sites for metastasis include lymph nodes, lung, liver and brain.

Metastatic prostate cancer is typically diagnosed by open or laparoscopic pelvic lymphadenectomy, whole body radionuclide scans, skeletal radiography, and/or bone lesion biopsy.

"Moderately stringent conditions" are described by, identified but not limited to, those in Sambrook et al., Molecular Cloning: A Laboratory Manual, New York: Cold Spring Harbor Press, 1989, and include the use of washing solution and hybridization conditions temperature, ionic strength and %SDS) less stringent than those described above. An example of moderately stringent conditions is overnight incubation at 370C in a solution comprising: 20% formamide, 5 x SSC (150 mM NaCI, 15 rmM trisodium citrate), 50 mM sodium phosphate (pH 5 x Denhardt's solution, 1 0% dextran sulfate, and 20 mg/mL denatured sheared salmon sperm DNA, followed by washing the filters in 1 x SSC at about 37-500C. The skilled artisan will recognize how to IN adjust the temperature, ionic strength, etc. as necessary to accommodate factors such as O probe length and the like.

U As used herein "motif" as in biological motif of an 84P2A9-realted protein, refers Sto any set of amino acids forming part of the primary sequence of a protein, either contiguous or capable of being aligned to certain positions that are generally invariant or conserved, that is associated with a particular function or modification that is phosphorylated, glycosylated or amidated).

As used herein, the term "polynucleotide" means a polymeric form of nucleotides of at least 10 bases or base pairs in length, either ribonucleotides or dcoxynuclcotides or N 10 a modified form of either type of nucleotide, and is meant to include single and double Sstranded forms of DNA and/or RNA. In the art, this term if often used interchangeably (1 with "oligonucleotide". As discussed herein, an polynucleotide can comprise a nucleotide sequence disclosed herein wherein thymidine (T (as shown for example in SEQ ID NO: 1) can also be uracil This description pertains to the differences between the chemical structures of DNA and RNA, in particular the observation that one of the four major bases in RNA is uracil instead of thymidine As used herein, the term "polypeptide" means a polymer of at least about 4, 5, 6, 7, or 8 amino acids. Throughout the specification, standard three letter or single letter designations for amino acids are used. In the art, this term if often used interchangeably with "peptide".

"Stringency" of hybridization reactions is readily determinable by one of ordinary skill in the art, and generally is an empirical calculation dependent upon probe length, washing temperature, and salt concentration. In general, longer probes require higher temperatures for proper annealing, while shorter probes need lower temperatures.

Hybridization generally depends on the ability of denatured nucleic acid sequences to reanneal when complementary strands are present in an environment below their melting temperature. The higher the degree of desired homology between the probe and hybridizable sequence, the higher the relative temperature that can be used. As a result, it follows that higher relative temperatures would tend to make the reaction conditions more stringent, while lower temperatures less so. For additional details and explanation O of stringency of hybridization reactions, sec Ausubel et al., Current Protocols in Molecular Biology, Wiley Interscience Publishers, (1995).

U "Stringent conditions" or "high stringency conditions", as defined herein, are Q identified by, but not limited to, those that: employ low ionic strength and high temperature for washing, for example 0.015 M sodium chloride/0.0015 M sodium citrate/0.1% sodium dodecyl sulfate at 50'C; employ during hybridization a denaturing agent, such as formamide, for example, 50% formamide with 0.1% bovine serum albumin/0.1% Ficoll/0.1% polyvinylpyrroldone/50mM sodium phosphate buffer at pH 6.5 with 750 mM sodium chloride, 75 mM sodium citrate at 42oC; or employ 50% formamide, 5 x SSC (0.75 M NaC1, 0.075 M sodium citrate), 0 mM sodium phosphate (PH 0.1% sodium pyrophosphate, 5 x Denhardt's solution,

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sonicated salmon sperm DNA (50 pig/ml), 0.1% SDS, and 10% dextran sulfate at 420C, with washes at 420C in 0.2 x SSC (sodium chloride/sodium, citrate) and 50% formamide at 550C, followed by a high-stringency wash consisting of 0.1 x SSC containing EDTA at 550C.

A "transgenic animal" a mouse or rat) is an animal having cells that contain a transgene, which transgene was introduced into the animal or an ancestor of the animal at a prenatal, an embryonic stage. A "transgene" is a DNA that is integrated into the genome of a cell from which a transgenic animal develops.

As used herein, the 84P2A9 gene and protein is meant to include the 84P2A9 genes and proteins specifically described herein and the genes and proteins corresponding to other 84P2A9 encoded proteins or peptides and structurally similar variants of the foregoing. Such other 84P2A9 peptides and variants will generally have coding sequences that are highly homologous to the 84P2A9 coding sequence, and preferably share at least about 50% amino acid homology (using BLAST criteria) and preferably 50%, 6 0 70%, 8 9 0 or more nucleic acid homology, and at least about amino acid homology (using BLAST criteria), more preferably sharing 70% or greater homology (using BLAST criteria).

The 84P2A9-related proteins of the invention include those specifically identified herein, as well as allelic variants, conservative substitution variants and homologs that can be isolated/generated and characterized without undue experimentation following the

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I methods outlined herein or are readily available in the art. Fusion proteins that combine Sparts of different 84P2A9 proteins or fragments thereof, as well as fusion proteins of an S84P2A9 protein and a heterologous polypepide are also included. Such 84P2A9 proteins Sare collectively referred to as the 84P2A9-related proteins, the proteins of the invention, or 84P2A9. As used herein, the term "84P2A9-related polypeptide" refers to a polypeptide fragment or an 84P2A9 protein sequence of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or more amino acids -l STRUCTURE AND EXPRESSION OF 84P2A9 As discussed in detail below, experiments with the LAPC-4 AD xenograft in Smale SCID mice have resulted in the identification of genes that are involved in the C1 progression of androgen dependent (AD) prostate cancer to androgen independent (AI) cancer. Briefly, mice that harbored LAPC-4 AD xenografts were castrated when the tumors reached a size of 1 cm in diameter. The tumors regressed in size and temporarily stopped producing the androgen dependent protein PSA. Seven to fourteen days postcastrauon, PSA levels were detectable again in the blood of the mice. Eventually such tumors develop an AI phenotype and start growing again in the castrated males. Tumors were harvested at different time points after castration to identify genes that are turned on or off during the transition to androgen independence.

Suppression subtractive hybridization (SSH) (Diatchenko et al., 1996, PNAS 93:6025) was then used to identify novel genes, such as those that are overexpressed in prostate cancer, by comparing cDNAs from various androgen dependent and androgen independent LAPC xenografts. This strategy resulted in the identification of novel genes exhibiting tissue and cancer specific expression. One of these genes, designated 84P2A9, was identified from a subtraction where cDNA derived from an LAPC-4 AD tumor, 3 days post-castration, was subtracted from cDNA derived from an LAPC-4 AD tumor grown in an intact male. The SSH DNA sequence of about 425 bp (Fig. 1) is novel and exhibits homology only to expressed sequence tags (ESTs) in the dbEST database.

84P2A9, encodes a putative nuclear protein that exhibits prostate and testisrelated expression. The initial characterization of 84P2A9 indicates that it is aberrantly expressed multiple cancers including prostate, testis, kidney, brain, bone, skin, ovarian, I breast, pancreas, colon, lymphocytic and lung cancers. The expression of 84P2A9 in prostate cancer provides evidence that this protein has a functional role in tumor U progression. It is possible that 84P2A9 functions as a transcription factor involved in Sactivating genes involved in tumorigenesis or repressing genes that block tumorigenesis.

As is further described in the Examples that follow, the 84P2A9 genes and proteins have been characterized using a number of analytical approaches. For example, analyses of nucleotide coding and amino acid sequences were conducted in order to identify potentially related molecules, as well as recognizable structural domains, topological features, and other elements within the 84P2A9 mRNA and protein 10 structures. Northern blot analyses of 84P2A9 mRNA expression were conducted in

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Sorder to establish the range of normal and cancerous tissues expressing 84P2A9 message.

C A full length 84P2A9 cDNA clone (clone 1) of 2345 base pairs (SEQ ID NO: 1) was cloned from an LAPC-4 AD cDNA library (Lambda ZAP Express, Stratagene) (Fig.

The cDNA encodes an open reading frame (ORF) of 504 amino acids (SEQ ID NO: Sequence analysis revealed the presence of six potential nuclear localization signals and is predicted to be nuclear using the PSORT program (http://psort.nibb.ac.jp:8800/form.html). The protein sequence has some homology to a human brain protein KIAA1152 (SEQ ID NO: 5) (39.5% identity over a 337 amino acid region), and contains a domain that is homologous to the LUCA15 tumor suppressor protein (SEQ ID NO: 6) (64.3% identity over a 42 amino acid region)(GenBank Accession #P52756)(Fig. 3).

84P2A9 expression is prostate and testis-related in normal adult human tissues, but is also expressed in certain cancers, including prostate, testis, kidney, brain, bone, skin, ovarian, breast, pancreas, colon, lymphocytic and lung cancers. (see, FIGS. 4- Human prostate tumor xenografts originally derived from a patient with high grade metastatic prostate cancer express high levels of 84P2A9 (FIG. 4).

As disclosed herein, 84P2A9 exhibits specific properties that are analogous to those found in a family of genes whose polynucleotides, polypeptides, reactive cytotoxic T cells (CTL), helper T cells (HTL) and anti-polypeptide antibodies are used in well known diagnostic assays directed to examining conditions associated with disregulated cell growth such as cancer, in particular prostate cancer (see, both its highly specific ki pattern of tissue expression as well as its overexpression in prostate cancers as described O for example in Example The best known member of this class is PSA, the archetypal 0 marker that has been used by medical practitioners for years to identify and monitor the Q presence of prostate cancer (see, Merrill et al., J. Urol. 163(2): 503-5120 (2000); Polascik et al., J. Urol. Aug;162(2):293-306 (1999) and Fortier et al.,J. Nat. Cancer Inst.

91(19): 1635-1640(1999)). A variety of other diagnostic markers are also used in this context including p 5 3 and K-ras (see, Tulchinsky et al., Int J Mol Med 1999 Jul;4(1):99-102 and Minimoto et al., Cancer Detect Prev 2000;24(1):1-12). Therefore, this disclosure of the 84P2A9 polynucleotides and polypeptides (as well as the 84P2A9 1 10 polynucleotide probes and anti-84P2A9 antibodies used to identify the presence of these

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O molecules) and their properties allows skilled artisans to utilize these molecules in C methods that are analogous to those used, for example, in a variety of diagnostic assays directed to examining conditions associated with cancer.

Typical embodiments of diagnostic methods which utilize the 84P2A9 polynucleotides, polypeptides and antibodies described herein are analogous to those methods from well established diagnostic assays which employ PSA polynucleotides, polypeptides and antibodies. For example, just as PSA polynucleotides are used as probes (for example in Northern analysis, see, Sharief et al., Biochem. Mol. Biol. Int.

33(3):567-74(1994)) and primers (for example in PCR analysis, see, Okegawa et al., J.

Urol. 163(4): 1189-1190 (2000)) to observe the presence and/or the level of PSA mRNAs m methods of monitoring PSA overexpression or the metastasis of prostate cancers, the 84P2A9 polynucleotides described herein can be utilized in the same way to detect 84P2A9 overexpression or the metastasis of prostate and other cancers expressing this gene. Alternatively, just as PSA polypeptides are used to generate antibodies specific for PSA which can then be used to observe the presence and/or the level of PSA proteins in methods of monitoring PSA protein overexpression (see, Stephan et al., Urology 55(4):560-3 (2000)) or the metastasis of prostate cells (see, Alanen et al., Pathol. Res. Pract. 192(3):233-7 (1996)), the 84P2A9 polypeptides described herein can be utilized to generate antibodies for use in detecting 84P2A9 overexpression or the metastasis of prostate cells and cells of other cancers expressing this gene.

ID Specifically, because metastases involves the movement of cancer cells from an 0 organ of origin (such as the testis or prostate gland etc.) to a different area of the body 0 (such as a lymph node), assays which examine a biological sample for the presence of Scells expressing 84P2A9 polynucleotides and/or polypeptides can be used to provide evidence of metastasis. For example, when a biological sample from tissue that does not normally contain 84P2A9 expressing cells (lymph node) is found to contain 84P2A9 expressing cells such as the 84P2A9 expression seen in LAPC4 and LAPC9, xenografts isolated from lymph node and bone metastasis, respectively, this finding is indicative of metastasis.

(C 10 Alternatively 84P2A9 polynucleotides and/or polypcptides can be used to

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O provide evidence of cancer, for example, when a cells in biological sample that do not C normally express 84P2A9 or express 84P2A9 at a different level are found to express 84P2A9 or have an increased expression of 84P2A9 (see, the 84P2A9 expression in kidney, lung and colon cancer cells and in patient samples etc. shown in Figures 4-10).

In such assays, artisans may further wish to generate supplementary evidence of metastasis by testing the biological sample for the presence of a second tissue restricted marker (in addition to 84P2A9) such as PSA, PSCA etc. (see, Alanen et al., Pathol.

Res. Pract. 192(3): 233-237 (1996)).

Just as PSA polynucleotide fragments and polynucleotide variants are employed by skilled artisans for use in methods of monitoring PSA, 84P2A9 polynucleotide fragments and polynucleotide variants are used in an analogous manner. In particular, typical PSA polynucleotides used in methods of monitoring PSA are probes or primers which consist of fragments of the PSA cDNA sequence. Illustrating this, primers used to PCR amplify a PSA polynucleotide must include less than the whole PSA sequence to function in the polymerase chain reaction. In the context of such PCR reactions, skilled artisans generally create a variety of different polynucleotide fragments that can be used as primers in order to amplify different portions of a polynucleotide of interest or to optimize amplification reactions (see, Cactano-Anolles, G. Biotechniques 25(3): 472- 476, 478-480 (1998); Robertson et al., Methods Mol. Biol. 98:121-154 (1998)). An additional illustration of the use of such fragments is provided in Example 3, where an 84P2A9 polynucleotide fragment is used as a probe to show the overexpression of

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I 84P2A9 mRlNAs in cancer cells. In addition, in order to facilitate their use by medical 1 practitioners, variant polynucleotide sequences are typically used as primers and probes U for the corresponding mRNAs in PCR and Northern analyses (see, Sawai et al., Fetal SDiagn. Ther. 1996 Nov-Dec;l1(6):407-13 and Current Protocols In Molecular Biology, Volume 2, Unit 2, Frederick M. Ausubul et al. eds., 1995)). Polynucleotide fragments and variants are typically useful in this context as long as they have the common attribute or characteristic of being capable of binding to a target polynucleotide sequence the S84P2A9 polynucleotide shown in SEQ ID NO: 1) under conditions of high stringency.

Just as PSA polypeptide fragments and polypeptide variants are employed by C1 10 skilled artisans for use in methods of monitoring the PSA molecule, 84P2A9 polypeptide 0 fragments and polypeptide variants can also be used in an analogous manner. In (1 particular, typical PSA polypeptides used in methods of monitoring PSA are fragments of the PSA protein which contain an antibody epitope that can be recognized by an antibody or T cell that specifically binds to the PSA protein. 'This practice of using polypeptide fragments or polypeptide variants to generate antibodies (such as anti-PSA antibodies or T cells) is typical in the art with a wide variety of systems such as fusion proteins being used by practitioners (see, Current Protocols In Molecular Biology, Volume 2, Unit 16, Frederick M. Ausubul et al. eds., 1995). In this context, each epitope(s) in a protein of interest functions to provide the architecture with which an antibody or T cell is reactive. Typically, skilled artisans generally create a variety of different polypeptide fragments that can be used in order to generate antibodies specific for different portions of a polypeptide of interest (see, U.S. Patent No. 5,840,501 and U.S. Patent No. 5,939,533). For example it may be preferable to utilize a polypeptide comprising one of the 84P2A9 biological motifs discussed herein or available in the art. Polypeptide fragments and variants or analogs are typically useful in this context as long as they comprise an epitope capable of generating an antibody or T cell specific for a target polypeptide sequence the 84P2A9 polypeptide shown in SEQ ID NO: 2).

As shown herein, the 84P2A9 polynucleotides and polypeptides (as well as the 84P2A9 polynucleotide probes and anti-84P2A9 antibodies or T cells used to identify the presence of these molecules) exhibit specific properties that make them useful in

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diagnosing cancers of the prostate. Diagnostic assays that measure the presence of 84P2A9 gene products, in order to evaluate the presence or onset of the particular disease conditions described herein such as prostate cancer are particularly useful in Sidentifying patients for preventive measures or further monitoring, as has been done so successfully with PSA. Moreover, these materials satisfy a need in the art for molecules having similar or complementary characteristics to PSA in situations where, for example, a definite diagnosis of metastasis of prostatic origin cannot be made on the basis of a Stesting for PSA alone (see, Alanen et al., Pathol. Res. Pract. 192(3): 233-237 (1996)), and consequently, materials such as 84P2A9 polynucleotides and polypeptides (as well as the 84P2A9 polynucleotide probes and anti-84P2A9 antibodies used to identify the Spresence of these molecules) must be employed to confirm metastases of prostatic origin.

"1 Finally, in addition to their use in diagnostic assays, the 84P2A9 polynucleotides disclosed herein have a number of other specific utilities such as their use in the identification of oncogenetic associated chromosomal abnormalities in 1q3 2 3 Moreover, in addition to their use in diagnostic assays, the 84P2A9-related proteins and polynucleotides disclosed herein have other utilities such as their use in the forensic analysis of tissues of unknown origin (see, Takahama K Forensic Sci Int 1996 Jun 28;80(1-2): 63-9).

84P2A9 POLYNUCLEOTIDES One aspect of the invention provides polynucleotides corresponding or complementary to all or part of an 84P2A9 gene, mRNA, and/or coding sequence, preferably in isolated form, including polynucleotides encoding an 84P2A9 protein and fragments thereof, DNA, RNA, DNA/RNA hybrid, and related molecules, polynucleotides or oligonucleotides complementary to an 84P2A9 gene or mRNA sequence or a part thereof, and polynucleotides or oligonucleotides that hybridize to an 84P2A9 gene, mRNA, or to an 84P2A9 encoding polynucleotide (collectively, "84P2A9 polynucleotides").

One embodiment of an 84P2A9 polynucleotide is an 84P2A9 polynucleotide having the sequence shown in SEQ ID NO: 1. An 84P2A9 polynucleotide can comprise

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N a polynucleotide having the nucleotide sequence of human 84P2A9 as shown in SEQ ID O NO: 1, wherein T can also be U; a polynucleotide that encodes all or part of the 84P2A9 protein; a sequence complementary to the foregoing; or a polynucleotide fragment of any Sof the foregoing. Another embodiment comprises a polynucleotide having the sequence as shown in SEQ ID NO: 1, from nucleotide residue number 163 through nucleotde residue number 1674, or from residue number 718 through residue number 1390, wherein T can also be U. Another embodiment comprises a polynucleotide encoding an 84P2A9 polypeptide whose sequence is encoded by the cDNA contained in the plasmid as deposited with American Type Culture Collection as Accession No. PTA-1151 Another embodiment comprises a polynucleoude that is capable of hybridizing under Sstringent hybridization conditions to the human 84P2A9 cDNA shown in SEQ ID NO: CI or to a polynucleotide fragment thereof.

Typical embodiments of the invention disclosed herein include 84P2A9 polynucleotides encoding specific portions of the 84P2A9 mRNA sequence (and those which are complementary to such sequences) such as those that encode the protein and fragments thereof, for example of 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or more contiguous amino acids. For example, representative embodiments of the invention disclosed herein include: polynucleotides encoding about amino acid position 1 to about amino acid 10 of the 84P2A9 protein shown in Fig. 2 (SEQ ID NO: polynucleotides encoding about amino acid 10 to about amino acid 20 of the 84P2A9 protein shown in Fig. 2, polynucleotides encoding about amino acid 20 to about amino acid 30 of the 84P2A9 protein shown in Fig. 2, polynucleotides encoding about amino acid 30 to about amino acid 40 of the 84P2A9 protein shown in Fig. 2, polynuclcotides encoding about amino acid 40 to about amino acid 50 of the 84P2A9 protein shown in Fig. 2, polynucleotides encoding about amino acid 50 to about amino acid 60 of the 84P2A9 protein shown in Fig. 2, polynucleotides encoding about amino acid 60 to about amino acid 70 of the 84P2A9 protein shown in Fig. 2, polynucleotides encoding about amino acid 70 to about amino acid 80 of the 84P2A9 protein shown in Fig. 2, polynucleotides encoding about amino acid 80 to about amino acid 90 of the 84P2A9 protein shown in Fig. 2 and polynucleotides encoding about amino acid 90 to about amino acid 100 of the 84P2A9 protein shown in Fig. 2, etc. Following this scheme, polynucleotides (of at least 0 10 nucleic acids) encoding portions of the amino acid sequence of amino acids 100-504 Sof the 84P2A9 protein are typical embodiments of the invention.

Polynucleotides encoding larger portions of the 84P2A9 protein are also Scontemplated. For example polynucleotides encoding from about anino acid 1 (or 20 or 30 or 40 etc.) to about amino acid 20, (or 30, or 40 or 50 etc.) of the 84P2A9 protein shown in Fig. 2 can be generated by a variety of techniques well known in the art. An illustrative embodiment of such a polynucleotide consists of a polynucleotide having the Ssequence as shown in FIG. 2, from nucleotide residue number 718 through nucleotide residue number 1390.

10 Additional illustrative embodiments of the invention disclosed herein include S84P2A9 polynucleotide fragments encoding one or more of the biological motifs Scontained within the 84P2A9 protein sequence. In one embodiment, typical polynucleotide fragments of the invention can encode one or more of the nuclear localization sequences disclosed herein. In another embodiment, typical polynuclcotide fragments of the invention can encode one or more of the regions of 84P2A9 that exhibit homology to LUCA 15 and/or KIAA1152 and/or NY-Lu-12 lung cancer antigen (AF 042857), which exhibits Zinc finger and RNA binding motifs (see, Gure et al., Cancer Res. 58(5): 1034-1041 (1998). In another embodiment of the invention, typical polynucleotide fragments can encode one or more of the 84P2A9 N-glycosylation sites, cAMP and cCMP-dependent protein kinase phosphorylation sites, casein kinase II phosphorylation sites or N-myristoylation site and amidation sites as disclosed in greater detail in the text discussing the 84P2A9 protein and polypeptides herein. In yet another embodiment of the invention, typical polynucleotide fragments can encode sequences that are unique to one or more 84P2A9 alternative splicing variants, such as the splice variant that generates the 4.5 KB transcript that is overexpressed in prostate cancers shown in FIG. 4.

The polynucleotides of the preceding paragraphs have a number of different specific uses. For example, because the human 84P2A9 gene maps to chromosome lq 3 2 3 polynucleotides encoding different regions of the 84P2A9 protein can be used to characterize cytogenetic abnormalities on chromosome 1, band q 3 2 that have been identified as being associated with various cancers. In particular, a variety of N chromosomal abnormalities in lq 3 2 including translocations and deletions have been identified as frequent cytogencuc abnormalities in a number of different cancers (see, Bieche et al., Genes Chromosomes Cancer, 24(3): 255-263 (1999); Gorunova et al., SGenes Chromosomes Cancer, 26(4): 312-321 (1999); Reid et al., Cancer Res. 5415- 1 5 5423 (1995)). Consequently, polynucleoides encoding specific regions of the 84P2A9 protein provide new tools that can be used to delineate with a greater precision than previously possible, the specific nature of the cytogenetic abnormalities in this region of Schromosome 1 that can contribute to the malignant phenotype. In this context, these polynucleotides satisfy a need in the art for expanding the sensitivity of chromosomal 10 screening in order to identify more subtle and less common chromosomal abnormalities

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0 (see, Evans et al., Am. J. Obstct. Gynecol 171(4): 1055-1057 (1994)).

C1 Alternatively, as 84P2A9 is shown to be highly expressed in prostate cancers (Fig.

these polynucleotides can be used in methods assessing the status of 84P2A9 gene products in normal versus cancerous tissues. Typically, polynucleotides encoding specific regions of the 84P2A9 protein can be used to assess the presence of perturbations (such as deletions, insertions, point mutations, or alterations resulting in a loss of an antigen etc.) in specific regions (such regions containing a nuclear localization signal) of the 84P2A9 gene products. Exemplary assays include both RT-PCR assays as well as single-strand conformation polymorphism (SSCP) analysis (see, Marrogi et al., J. Cutan. Pathol. 26(8): 369-378 (1999), both of which utilize polynucleotides encoding specific regions of a protein to examine these regions within the protein.

Other specifically contemplated nucleic acid related embodiments of the invention disclosed herein are genomic DNA, cDNAs, ribozymes, and antisense molecules, as well as nucleic acid molecules based on an alternative backbone or including alternative bases, whether derived from natural sources or synthesized. For example, antisense molecules can be RNAs or other molecules, including peptide nucleic acids (PNAs) or non-nucleic acid molecules such as phosphorothioate derivatives, that specifically bind DNA or RNA in a base pair-dependent manner. A skilled artisan can readily obtain these classes of nucleic acid molecules using the 84P2A9 polynucleotdes and polynucleotide sequences disclosed herein.

SAntisense technology entails the administration of exogenous oligonucleocides Sthat bind to a target polynuclcotide located within the cells. The term "antisense" refers 0 to the fact that such oligonucleotides are complementary to their intracellular targets, Se.g., 84P2A9. See for example, Jack Cohen, OLIGODEOXYNUCLEOTIDES, Antisense Inhibitors of Gene Expression, CRC Press, 1989; and Synthesis 1:1-5 (1988).

The 84P2A9 antisense oligonucleotides of the present invention include derivatives such as S-oligonucleotides (phosphorothioate derivatives or S-oligos, see, Jack Cohen, supra), which exhibit enhanced cancer cell growth inhibitory action. S-oligos (nuclcoside phosphorothioates) are isoelectronic analogs of an oligonucleotide (O-oligo) in which a nonbridgng oxygen atom of the phosphate group is replaced by a sulfur atom. The S-

NO

O ohgos of the present invention can be prepared by treatment of the corresponding O- C oligos with 3H-1,2-benzodithiol-3-one-1,1-dioxide, which is a sulfur transfer reagent.

See Iyer, R. P. et al, J. Org. Chem. 55:4693-4698 (1990); and lycr, R. P. et al., J. Am.

Chem. Soc. 112:1253-1254 (1990). Additional 84P2A9 antisense oligonucleotdes of the present invention include morpholino antisense oligonucleotides known in the art (see, Partridge ct al., 1996, Antisense Nucleic Acid Drug Development 6: 169-175).

The 84P2A9 antisense oligonucleotides of the present invention typically can be RNA or DNA that is complementary to and stably hybridizes with the first 100 Nterminal codons or last 100 C-terminal codons of the 84P2A9 genomic sequence or the corresponding mRNA. Absolute complementarity is not required, although high degrees of complementarity are preferred. Use of an oligonucleotide complementary to this region allows for the selective hybridization to 84P2A9 mRNA and not to mRNA specifying other regulatory subunits of protein kinase. Preferably, the 84P2A9 antisense oligonucleotides of the present invention are a 15 to 30-mer fragment of the antisense DNA molecule having a sequence that hybridizes to 84P2A9 mRNA. Optionally, 84P2A9 antisense oligonuclcotide is a 30-mer oligonucleotide that is complementary to a region in the first 10 N-terminal codons or last 10 C-termnal codons of 84P2A9.

Alternatively, the antisense molecules are modified to employ ribozymes in the inhibition of 84P2A9 expression. L. A. Couture D. T. Stinchcomb; Trends Genet 12: 510-515 (1996).

22 D Further specific embodiments of this aspect of the invention include primers and Sprimer pairs, which allow the specific amplification of the polynucleotides of the O invention or of any specific parts thereof, and probes that selectively or specifically Shybridize to nucleic acid molecules of the invention or to any part thereof. Probes can be labeled with a detectable marker, such as, for example, a radioisotope, fluorescent compound, bioluminescent compound, a chemiluminescent compound, metal chelator or enzyme. Such probes and primers can be used to detect the presence of an 84P2A9 polynucleotide in a sample and as a means for detecting a cell expressing an 84P2A9 protein.

N 10 Examples of such probes include polypeptides comprising all or part of the human S84P2A9 cDNA sequences shown in FIG. 2. Examples of primer pairs capable of C, specifically amplifying 84P2A9 mRNAs are also described in the Examples that follow. As will be understood by the skilled artisan, a great many different primers and probes can be prepared based on the sequences provided herein and used effectively to amplify and/or detect an 84P2A9 mRNA.

The 84P2A9 polynucleotides of the invention are useful for a variety of purposes, including but not limited to their use as probes and primers for the amplification and/or detection of the 84P2A9 gene(s), mRNA(s), or fragments thereof; as reagents for the diagnosis and/or prognosis of prostate cancer and other cancers; as coding sequences capable of directing the expression of 84P2A9 polypeptides; as tools for modulating or inhibiting the expression of the 84P2A9 gene(s) and/or translation of the 84P2A9 transcript(s); and as therapeutic agents.

ISOLATION OF 84P2A9-ENCODING NUCLEIC ACID MOLECULES The 84P2A9 cDNA sequences described herein enable the isolation of other polynucleotides encoding 84P2A9 gene product(s), as well as the isolation of polynucleotides encoding 84P2A9 gene product homologs, alternatively spliced isoforms, allelic variants, and mutant forms of the 84P2A9 gene product. Various molecular cloning methods that can be employed to isolate full length cDNAs encoding an 84P2A9 gene are well known (See, for example, Sambrook, J. et al., Molecular Cloning: A Laboratory Manual, 2d edition., Cold Spring Harbor Press, New York, 1989; Current Protocols in O Molecular Biology. Ausubel et al., Eds., Wiley and Sons, 1995). For example, lambda O phage cloning methodologies can be conveniently employed, using commercially available Scloning systems Lambda ZAP Express, Stratagene). Phage clones containing 84P2A9 gene cDNAs can be identified by probing with a labeled 84P2A9 cDNA or a fragment thereof. For example, in one embodiment, the 84P2A9 cDNA (FIG. 2) or a portion thereof can be synthesized and used as a probe to retrieve overlapping and full length cDNAs corresponding to an 84P2A9 gene. The 84P2A9 gene itself can be isolated by screening genomic DNA libraries, bacterial artificial chromosome libraries (BACs), yeast CN 10 artificial chromosome libraries (YACs), and the like, with 84P2A9 DNA probes or primers.

RECOMBINANT DNA MOLECULES AND HOST-VECTOR SYSTEMS The invention also provides recombinant DNA or RNA molecules containing an 84P2A9 polynucleotide or a fragment or analog or homologue thereof, including but not limited to phages, plasmids, phagemids, cosmids, YACs, BACs, as well as various viral and non-viral vectors well known in the art, and cells transformed or transfected with such recombinant DNA or RNA molecules. As used herein, a recombinant DNA or RNA molecule is a DNA or RNA molecule that has been subjected to molecular manipulation in vitro. Methods for generating such molecules are well known (see, for example, Sambrook et al, 1989, supra).

The invention further provides a host-vector system comprising a recombinant DNA molecule containing an 84P2A9 polynucleotide or fragment or analog or homologue thereof within a suitable prokaryotic or eukaryotic host cell. Examples of suitable eukaryotic host cells include a yeast cell, a plant cell, or an animal cell, such as a mammalian cell or an insect cell a baculovirus-infcctible cell such as an Sf9 or HighFive cell). Examples of suitable mammalian cells include various prostate cancer cell lines such as DU145 and TsuPrl, other transfectable or transducible prostate cancer cell lines, as well as a number of mammalian cells routinely used for the expression of recombinant proteins COS, CHO, 293, 293T cells). More particularly, a polynucleotide comprising the coding sequence of 84P2A9 or a fragment or analog or

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I homolog thereof can be used to generate 84P2A9 proteins or fragments thereof using any Snumber of host-vector systems routinely used and widely known in the art.

U A wide range of host-vector systems suitable for the expression of 84P2A9 proteins Sor fragments thereof are available, see for example, Sambrook et al., 1989, supra; Current Protocols in Molecular Biology, 1995, supra). Preferred vectors for mammalian expression include but are not limited to pcDNA 3.1 myc-His-tag (Invitrogen) and the retroviral vector pSRottkneo (Muller et al., 1991, MCB 11:1785). Using these expression Svectors, 84P2A9 may be preferably expressed in several prostate cancer and non-prostate cell lines, including for example 293, 293T, rat-1, NIH 3T3 and TsuPrl. The host-vector O 10 systems of the invention are useful for the production of an 84P2A9 protein or fragment Sthereof. Such host-vector systems can be employed to study the functional properties of

C

84P2A9 and 84P2A9 mutations or analogs.

Recombinant human 84P2A9 protein or an analog or homolog or fragment thereof can be produced by mammalian cells transfected with a construct encoding 84P2A9. In an illustrative embodiment described in the Examples, 293T cells can be transfected with an expression plasmid encoding 84P2A9 or fragment or analog or homolog thereof, the 84P2A9 or related protein is expressed in the 293T cells, and the recombinant 84P2A9 protein can be isolated using standard purification methods affinity purification using anti-84P2A9 antibodies). In another embodiment, also described in the Examples herein, the 84P2A9 coding sequence is subcloned into the retroviral vector pSRccMSVtkneo and used to infect various mammalian cell lines, such as NIH 3T3, TsuPrl, 293 and rat-1 in order to establish 84P2A9 expressing cell lines.

Various other expression systems well known in the art can also be employed.

Expression constructs encoding a leader peptide joined m frame to the 84P2A9 coding sequence can be used for the generation of a secreted form of recombinant 84P2A9 protein.

Proteins encoded by the 84P2A9 genes, or by analogs or homologs or fragments thereof, will have a variety of uses, including but not limited to generating antibodies and in methods for identifying ligands and other agents and cellular constituents that bind to an 84P2A9 gene product. Antibodies raised against an 84P2A9 protein or fragment thereof can be useful in diagnostic and prognostic assays, and imaging methodologies in

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N the management of human cancers characterized by expression of 84P2A9 protein, including but not limited to cancers of the prostate and testis. Such antbodies can be U expressed intracellularly and used in methods of treating patients with such cancers.

SVarious immunological assays useful for the detection of 84P2A9 proteins are 5 contemplated, including but not limited to various types of radioimmunoassays, enzymelinked immunosorbent assays (ELISA), enzyme-linked ummunofluorescent assays (ELIFA), immunocytochcmical methods, and the like. Such antibodies can be labeled and used as immunological imaging reagents capable of detecting 84P2A9 expressing cells in radioscintigraphic imaging methods). 84P2A9 proteins can also be particularly useful m generanng cancer vaccines, as further described below.

84P2A9 POLYPEPTIDES Another aspect of the present invention provides 84P2A9-related proteins and polypeptide fragments thereof. Specific embodiments of 84P2A9 proteins comprise a polypeptide having all or part of the amino acid sequence of human 84P2A9 as shown in FIG. 2. Alternatively, embodiments of 84P2A9 proteins comprise variant polypeptides having alterations in the amino acid sequence of human 84P2A9 shown in FIG. 2.

In general, naturally occurring allelic variants of human 84P2A9 share a high degree of structural identity and homology 9 0% or more identity). Typically, allelic variants of the 84P2A9-related proteins contain conservative amino acid substitutions within the 84P2A9 sequences described herein or contain a substitution of an amino acid from a corresponding position in a homologue of 84P2A9. One class of 84P2A9 allelic variants are proteins that share a high degree of homology with at least a small region of a particular 84P2A9 amino acid sequence, but further contain a radical departure from the sequence, such as a non-conservative substitution, truncation, insertion or frame shift. In comparisons of protein sequences, the terms, Similarity, identity, and Homology each have a distinct meaning. Moreover, Orthology and Paralogy are important concepts describing the relationship of members of a given protein family in one organism to the members of the same family in other organisms.

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Conservative amino acid substtutions can frequently be made in a protein Swithout altering either the conformation or the function of the protein. Such changes 0 include substituting any of isoleucine valine and leucine for any other of these Shydrophobic amino acids; aspartic acid for glutamic acid and vice versa; glutamine for asparagine and vice versa; and serine for threonme and vice versa. Other substitutions can also be considered conservative, depending on the environment of the particular amino acid and its role in the three-dimensional structure of the protein. For example, glycine and alanine can frequently be interchangeable, as can alanine and valine Methionine which is relatively hydrophobic, can frequently be interchanged with leucine and isoleucine, and sometimes Swith valine. Lysine and arginie are frequently interchangeable in locations in C1 which the significant feature of the amino acid residue is its charge and the differing pK's of these two amino acid residues are not significant. Still other changes can be considered "conservative" in particular environments (see, e.g. Table 2 herein; pages 13- 15 "Biochemistry" 2 nd ED. Lubert Strycr ed (Stanford University); Henikoff et al., PNAS 1992 Vol 89 10915-10919; Lei et al.,J Biol Chem 1995 May 19; 270(20):11882-6).

Embodiments of the invention disclosed herein include a wide variety of art accepted variants of 84P2A9 proteins such as polypeptides having amino acid insertions, deletions and substitutions. 84P2A9 variants can be made using methods known in the art such as site-directed mutagenesis, alanine scanning, and PCR mutagenesis. Sitedirected mutagenesis [Carter et al., Nuc/. Acids Res., 13:4331 (1986); Zoller et al., Nucl.

Acids Res., 10:6487 (1987)], cassette mutagenesis [Wells et al., Gene, 34:315 (1985)], restriction selection mutagenesis [Wells et al., Philos. Trans. R. Soc. London SerA, 317:415 (1986)] or other known techniques can be performed on the cloned DNA to produce the 84P2A9 variant DNA.

Scanning amino acid analysis can also be employed to identify one or more amino acids along a contiguous sequence that is involved in a specific biological activity such as a protein-protein interaction. Among the preferred scanning amino acids are relatively small, neutral amino acids. Such amino acids include alanine, glycine, serine, and cysteine. Alanine is typically a preferred scanning amino acid among this group because it eliminates the side-chain beyond the beta-carbon and is less likely to alter the 27

I

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main-chain conformation of the variant. Alanine is also typicaly preferred because it is the most common amino acid. Further, it is frequently found in both buried and U exposed positions [Creighton, The Proteins, Freeman Co., Chothia, J. Mol.

SBiol., 150:1 (1976)]. If alanine substitution does not yield adequate amounts of variant, an isosteric amino acid can be used.

As defined herein, 84P2A9 variants, analogs or homologs, have the distinguishing attribute of having at least one epitope in common with an 84P2A9 protein having the amino acid sequence of SEQ ID NO: 2, such that an antibody or T cell that specifically binds to an 84P2A9 variant will also specifically bind to the 84P2A9 protein having the amino acid sequence of SEQ ID NO: 2. A polypeptide ceases to be a variant of the Sprotein shown in SEQ ID NO: 2 when it no longer contains an epitope capable of being Srecognized by an antibody or T cell that specifically binds to an 84P2A9 protein. Those skilled in the art understand that antibodies that recognize proteins bind to epitopes of varying size, and a grouping of the order of about four or five amino acids, contiguous or not, is regarded as a typical number of amino acids in a minimal epitope. See, Nair et al., J. Immunol 2000 165(12): 6949-6955; Hebbes et al., Mol Immunol (1989) 26(9):865-73; Schwartz et al., J Immunol (1985) 135(4):2598-608. Another specific class of 84P2A9-related protein variants shares 9 0% or more identity with the amino acid sequence of SEQ ID NO: 2 or a fragment thereof. Another specific class of 84P2A9 protein variants or analogs comprise one or more of the 84P2A9 biological motifs described below or presently known in the art. Thus, encompassed by the present invention are analogs of 84P2A9 fragments (nucleic or amino acid) that altered functional immunogenic) properties relative to the starting fragment. It is to be appreciated that motifs now or which become part of the art are to be applied to the nucleic or amino acid sequences of FIG. 2.

As discussed herein, embodiments of the claimed invention include polypeptides containing less than the 504 amino acid sequence of the 84P2A9 protein shown in FIG.

2. For example, representative embodiments of the invention comprise peptides/proteins having any 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or more contiguous amino acids of the 84P2A9 protein shown in Fig. 2 (SEQ ID NO: Moreover, representative embodiments of the invention disclosed herein include polypeptides consisting of about amino acid 1 to about amino acid 10 of the 84P2A9 protein shown in SFig. 2, polypeptides consisting of about amino acid 10 to about amino acid 20 of the U 84P2A9 protein shown in Fig. 2, polypeptides consisting of about amino acid 20 to Sabout amino acid 30 of the 84P2A9 protein shown m Fig. 2, polypeptides consisting of 1> 5 about amino acid 30 to about amino acid 40 of the 84P2A9 protein shown in Fig. 2, polypeptides consisting of about amino acid 40 to about amino acid 50 of the 84P2A9 protein shown in Fig. 2, polypeptides consisting of about amino acid 50 to about amino Sacid 60 of the 84P2A9 protein shown in Fig. 2, polypeptides consisting of about amino acid 60 to about amino acid 70 of the 84P2A9 protein shown in Fig. 2, polypeptides C1 10 consisting of about amino acid 70 to about amino acid 80 of the 84P2A9 protein shown

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Sin Fig. 2, polypeptides consisting of about amino acid 80 to about amino acid 90 of the (C1 84P2A9 protein shown in Fig. 2 and polypeptides consisting of about amino acid 90 to about amino acid 100 of the 84P2A9 protein shown in Fig. 2, etc. throughout the entirety of the 84P2A9 sequence. Following this scheme, polypeptides consisting of portions of the amino acid sequence of amino acids 100-504 of the 84P2A9 protein are typical embodiments of the invention. Polypeptides consisting of larger portions of the 84P2A9 protein are also contemplated. For example polypeptides consisting of about amino acid 1 (or 20 or 30 or 40 etc.) to about amino acid 20, (or 30, or 40 or 50 etc.) of the 84P2A9 protein shown in Fig. 2 can be generated by a variety of techniques well known in the art. It is to be apprecited that the starting and stopping positions in this paragraph refer to the specified position as well as that position plus or minus 5 residues.

Additional illustrative embodiments of the invention disclosed herein include 84P2A9-related proteins containing the amino acid residues of one or more of the biological motifs contained within the 84P2A9-related protein sequence as shown in Figure 2. In one embodiment, proteins of the invention comprise one or more of the 84P2A9 nuclear localization sequences such as RKRR at residues 42-45 of SEQ ID NO: 2, RKRR at residues 47-50 of SEQ ID NO: 2, KRRP at residues 101-104 of SEQ ID NO: 2, RR1URRK at residues 135-139 of SEQ ID NO: 2 and/or KKRK at residues 186- 189 of SEQ ID NO: 2. In another embodiment, proteins of the invention comprise one or more of the 84P2A9 N-glycosylation sites such as NRTL at residues 131-134 of SEQ ID NO: 2, NQTN at residues 212-215 of SEQ ID NO: 2 and/or NCSV at residues 394- S397 of SEQ ID NO: 2. In another embodiment, proteins of the invention comprise one 1 or more of the regions of 84P2A9 that exhibit homology to LUCA 15 and/or K [KIAA1152. In another embodiment, proteins of the invention comprise one or more of Sthe 84P2A9 cAMP and cGMP-dependent protein kinase phosphorylation sites such as I" 5 KRRS at residues 48-51 of SEQ ID NO: 2 and/or RRPS at residues 102-105 of SEQ ID NO: 2. In another embodiment, proteins of the invention comprise one or more of the 84P2A9 Protein Kinase C phosphorylation sites such as TLR at residues 133-135 of SEQ ID NO: 2, SNK at residues 152-154 of SEQ ID NO: 2, SDR at residues 171-173 of SEQ ID NO: 2, TNK at residues 214-216 of SEQ ID NO: 2, SRR at residues 313-315 10 of SEQ ID NO: 2, SSK at residues 328-330 of SEQ ID NO: 2 and/or SVR at residues S396-398 of SEQ ID NO: 2. In another embodiment, proteins of the invention comprise C1 one or more of the 84P2A9 casein kinase II phosphorylation sites such as SALE at residues 10-13 of SEQ ID NO: 2, SSLE at residues 70-73 of SEQ ID NO: 2, SLEE at residues 71-74 of SEQ ID NO: 2, SDSD at residues 91-94 of SEQ ID NO: 2, TNKD at residues 214-217 of SEQ ID NO: 2, SESD at residues 232-235 of SEQ ID NO: 2, SSTD at residues 240-243 of SEQ ID NO: 2, TNDE at residues 248-251 of SEQ ID NO: 2, TELD at residues 287-290 of SEQ ID NO: 2 and/or TEHD at residues 374-377 of SEQ ID NO: 2. In another embodiment, proteins of the invention comprise one or more of the N-myristoylation sites such as GSDSSL at residues 67-72 of SEQ ID NO: 2, GLFTND at residues 245-250 of SEQ ID NO: 2, GGACGI at residues 269-274 of SEQ ID NO: 2, GGTPTS at residues 336-341 of SEQ ID NO: 2, GTPTSM at residues 337- 342 of SEQ ID NO: 2, GSLCTG at residues 409-414 of SEQ ID NO: 2, GSGLGR at residues 459-464 of SEQ ID NO: 2 and/or GLGLGF at residues 481-486 of SEQ ID NO: 2. In another embodiment, proteins of the invention comprise one or more amidation sites such as RGRK at residues 45-48 of SEQ ID NO: 2 and/or RGKR at residues 113-116 of SEQ ID NO: 2. An illustrative embodiment of such a polypeptide includes two or more amino acid sequences selected from the group consisting of KKRK, NQTN, NCSV, TNK, SRR, SSK, SVR, GLFTND, GGACGI, GGTPTS, GTPTSM and GSLCTG (as identified above in SEQ ID NO: In a preferred embodiment, the polypeptide comprises three or four or five or six or more amino acid sequences KKRK, NQTN, NCSV, TNK, SRR, SSK, SVR, GLFTND, GGACGI, GGTPTS, GTPTSM and GSLCTG (as identified above in SEQ ID NO: 2).

O In another embodiment, proteins of the invention comprise one or more of the 0 immunoreactive epitopes identified by a process described herein such as such as those Sshown in Table 1. Processes for identifying peptides and analogues having affinities for 5 HLA molecules and which are correlated as immunogenic epitopes, are well known in the art. Also disclosed are principles for creating analogs of such epitopes in order to modulate immunogenicity. A variety of references are useful in the identification of such molecules. See, for example, WO 9733602 to Chestnut et al.; Sette, Immunogenetics 1999 50(3-4): 201-212; Sette et al., J. Immunol. 2001 166(2): 1389-1397; Alexander et al., C1 10 Immunol. Res. 18(2): 79-92; Sidney et al., Hum. Immunol. 1997 58(1): 12-20; Kondo et O al., Immunogenetics 1997 45(4): 249-258; Sidney ct al., J. Immunol. 1996 157(8): 3480- CK, 90; and Falk et al., Nature 351: 290-6 (1991); Hunt ct al., Science 255:1261-3 (1992); Parker et al., J. Immunol. 149:3580-7 (1992); Parker et al., J. Immunol. 152:163-75 (1994)); Kast et al., 1994 152(8): 3904-12; Borras-Cuesta et al., Hum. Immunol. 2000 61(3): 266-278; Alexander et al., J. Immunol. 2000 164(3); 164(3): 1625-1633; Alexander et al., PMID: 7895164, UI: 95202582; O'Sullivan et al., J. Immunol. 1991 147(8): 2663- 2669; Alexander et al., Immunity 1994 751-761 and Alexander et al., Immunol. Res.

1998 18(2): 79-92. The disclosures of these publications are hereby incorporated by reference herein in their entireties.

Related embodiments of the invention comprise polypeptides containing combinations of the different motifs discussed herein, where certain embodiments contain no insertions, deletions or substitutions either within the motifs or the intervening sequences of these polypeptides. In addition, embodiments which include a number of either N-terminal and/or C-terminal amino acid residues on either side of these motifs may be desirable (to, for example, include a greater portion of the polypeptide architecture in which the motif is located). Typically the number of Nterminal and/or C-terminal amino acid residues on either side of a motif is between about 1 to about 100 amino acid residues, preferably 5 to about 50 amino acid residues.

In another embodiment of the invention, proteins of the invention comprise amino acid sequences that are unique to one or more 84P2A9 alternative splicing variants, such as the splice variant encoded by the 4.5 KB transcript that is overexpressed D in prostate cancers and shown in FIG. 4. The monitoring of alternative splice variants of S84P2A9 is useful because changes in the alternative splicing of proteins is suggested as 0 one of the steps in a series of events that lead to the progression of cancers (see, e.g., SCarstens et al., Oncogene 15(250: 3059-3065 (1997)). Consequently, monitoring of alternative splice variants of 84P2A9 provides an additional means to evaluate syndromes associated with perturbations in 84P2A9 gene products such as cancers.

Polypeptides comprising one or more of the 84P2A9 motifs discussed herein are Suseful in elucidating the specific characteristics of a malignant phenotype in view of the observation that the 84P2A9 motifs discussed herein are associated with growth r, 10 disregulation and because 84P2A9 is overexpressed in cancers (FIG. Thus, the O presence in a protein of motifs related to these enzymes or molecules is relevant. For C example, Casein kinase II, cAMP and cCMP-dependent protein kinase and Protein Kinase C for example are enzymes known to be associated with the development of the malignant phenotype (see, Chen et al., Lab Invest., 78(2): 165-174 (1998); Gaiddon et al., Endocrinology 136(10): 4331-4338 (1995); Hall et al., Nucleic Acids Research 24(6): 1119-1126 (1996); Peterziel et al., Oncogene 18(46): 6322-6329 (1999) and O'Brian, Oncol. Rep. 305-309 (1998)). Moreover, both glycosylation and myristylation are protein modifications also associated with cancer and cancer progression (see, Dennis et al., Biochim. Biophys. Acta 1473(1):21-34 (1999); Raju et al., Exp. Cell Res. 235(1): 145-154 (1997)). Amidation is another protein modification associated with cancer and cancer progression (see, Treston et al., J. Natl. Cancer Inst. Monogr. 169-175 (1992)). In addition, nuclear localization sequences are believed to influence the malignant potential of a cell (see, Mirski et al., Cancer Res.

55(10): 2129-2134 (1995)).

The proteins of the invention have a number of different specific uses. As 84P2A9 is shown to be highly expressed in prostate cancers (Fig. these peptides/proteins are used in methods assessing the status of 84P2A9 gene products in normal versus cancerous tissues and elucidating the malignant phenotype. Typically, polypeptides encoding specific regions of the 84P2A9 protein are used to assess the presence of perturbations (such as deletions, insertions, point mutations etc.) in specific regions (such regions containing a nuclear localization signal) of the 84P2A9 gene

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C products. Exemplary assays utilize antibodies or T cells targeting 84P2A9-related proteins comprising the amino acid residues of one or more of the biological motifs U contained within the 84P2A9 polypeptide sequence in order to evaluate the Scharacteristics of this region in normal versus cancerous tissues. Alternatively, 84P2A9 polypeptides containing the amino acid residues of one or more of the biological motifs contained within the 84P2A9 proteins are used to screen for factors that interact with that region of 84P2A9.

SAs discussed herein, redundancy in the genetic code permits variation in 84P2A9 gene sequences. In particular, one skilled in the art will recognize specific codon preferences by a specific host species, and can adapt the disclosed sequence as preferred Sfor a desired host. For example, preferred analog codon sequences typically have rare C codons codons having a useage frequency of less than about 20% in known sequences of the desired host) replaced with higher frequency codons. Codon preferences for a specific species are calculated, for example, by utilizing codon usage tables available on the INTERNET such as: http://www.dna.affrc.go.jp/-nakamura/codon.html. Nucleotide sequences that have been optimized for a particular host species by replacing any codons having a useage frequency of less than about 20% are referred to herein as "codon optimized sequences." Additional sequence modifications are known to enhance protein expression in a cellular host. These include elimination of sequences encoding spurious polyadenylation signals, exon/intron splice site signals, transposon-like repeats, and/or other such wellcharacterized sequences that are deleterious to gene expression. The GC content of the sequence is adjusted to levels average for a given cellular host, as calculated by reference to known genes expressed in the host cell. Where possible, the sequence is modified to avoid predicted hairpin secondary mRNA structures. Other useful modifications include the addition of a translational initiation consensus sequence at the start of the open reading frame, as described in Kozak, Mol. Cell Biol., 9:5073-5080 (1989). Skilled artisans understand that the general rule that eukaryotic ribosomes initiate translation exclusively at the 5' proximal AUG codon is abrogated only under rare conditions (see, Kozak PNAS 92(7): 2662-2666, (1995) and Kozak NAR 15(20): 8125-8148 (1987)). Nucleotide sequences that have been optimized for expression in a given host species by elimination ID of spurious polyadenylation sequences, elimination of exon/intron splicing signals, Selimination of transposon-like repeats and/or optimization of GC content in addition to O codon optimization are referred to herein as an "expression enhanced sequence." S84P2A9 proteins are embodied in many forms, preferably in isolated form. A 5 purified 84P2A9 protein molecule will be substantially free of other proteins or molecules that impair the binding of 84P2A9 to antibody or other ligand. The nature and degree of isolation and purification will depend on the intended use. Embodiments of an 84P2A9 protein include a purified 84P2A9 protein and a functional, soluble 84P2A9 protein. In one embodiment, a functional, soluble 84P2A9 protein or fragment thereof C 10 retains the ability to be bound by antibody, T cell or other ligand.

O The invention also provides 84P2A9 proteins comprising biologically active C fragments of the 84P2A9 amino acid sequence corresponding to part of the 84P2A9 amino acid sequence shown in FIG. 2. Such proteins of the invention exhibit properties of the 84P2A9 protein, such as the ability to elicit the generation of antbodies that specifically bind an epitope associated with the 84P2A9 protein; to be bound by such antibodies; to elicit the activation of I-ITL or CTL; and/or, to be recognized by HTL or

CTL.

84P2A9-related proteins are generated using standard peptde synthesis technology or using chemical cleavage methods well known in the art. Alternatively, recombinant methods can be used to generate nucleic acid molecules that encode an 84P2A9-related protein. In one embodiment, the 84P2A9-encoding nucleic acid molecules described herein provide means for generating defined fragments of 84P2A9 proteins. 84P2A9 protein fragments/subsequences are particularly useful in generating and characterizing domain specific antibodies antibodies recognizing an extracellular or intracellular epitope of an 84P2A9 protein), in identifying agents or cellular factors that bind to 84P2A9 or a particular structural domain thereof, and in various therapeutic contexts, including but not limited to cancer vaccines or methods of preparing such vaccines.

84P2A9 polypeptides containing particularly interesting structures can be predicted and/or identified using various analytical techniques well known in the art, including, for example, the methods of Chou-Fasman, Garmer-Robson, Kyte-Doolittle, Eisenberg, Karplus-Schultz or Jameson-Wolf analysis, or on the basis of immunogenicity. Fragments O containing such structures are particularly useful in generating subunit specific ann-84P2A9 Santibodies, or T cells or in identifying cellular factors that bind to 84P2A9.

SIllustrating this, the binding of peptides from 84P2A9 proteins to the human SMHC class I molecule HLA-A2 were predicted. Specifically, the complete amino acid sequence of the 84P2A9 protein was entered into the HLA Peptide Motif Search algorithm found in the Bioinformatics and Molecular Analysis Section (BIMAS) Web site (http://bimas.dcrt.nih.gov/). The HLA Pepude Motif Search algorithm was developed by Dr. Ken Parker based on binding of specific peptide sequences in the groove of HLA Class I molecules and specifically HLA-A2 (see, Falk et al., Nature 351: 290-6 C, 10 (1991); Hunt et al., Science 255:1261-3 (1992); Parker et al., J. Immunol. 149:3580-7

NO

O (1992); Parker et al., J. Immunol. 152:163-75 (1994)). This algorithm allows location and C1 ranking of 8-mer, 9-mer, and 10-mer peptides from a complete protein sequence for predicted binding to HLA-A2 as well as numerous other HLA Class I molecules. Many HLA class I binding peptides are 10 or 11-mers. For example, for class I HLA- A2, the epitopes preferably contain a leucine or methionine at position 2 and a valine or leucine at the C-terminus (see, Parker et al., J. Immunol. 149:3580-7 (1992)). Selected results of 84P2A9 predicted binding peptides are shown in Table 1 below. It is to be appreciated that every epitope predicted by the DIMAS site, or specified by the HLA class I or class I motifs available in the art are to be applied visually or by computer based methods, or appreciated by those of skill in the relevant art) or which become part of the art are within the scope of the invention. In Table 1, the top 10 ranking candidates for each family member are shown along with their location, the amino acid sequence of each specific peptide, and an estimated binding score. The binding score corresponds to the estimated half-time of dissociation of complexes containing the peptide at 370C at pH 6.5. Peptides with the highest binding score 63.04 for 84P2A9) are predicted to be the most tighty bound to HLA Class I on the cell surface for the greatest period of time and thus represent the best immunogenic targets for T-cell recognition. Actual binding of peptides to an HLA allele can be evaluated by stabilization of HLA expression on the antigen-processing defective cell line T2 (see, Xue et al., Prostate 30:73-8 (1997) and Peshwa et al., Prostate 36:129-38 (1998)). Immunogenicity of specific peptides can be evaluated m vitro by stimulation of CD8+ cytotoxic T lymphocytes (CTL) in the presence of antigen presenting cells such as dendritic cells.

0 In an embodiment described in the examples that follow, 84P2A9 can be Sconveniently expressed in cells (such as 293T cells) transfected with a commercially available expression vector such as a CMV-driven expression vector encoding 84P2A9 with a C-terminal 6XHis and MYC tag (pcDNA3.1/mycHIS, Invitrogen or GenHunter Corporation, Nashville TN). The Tag5 vector provides an IgGK secretion signal that can be used to facilitate the production of a secreted 84P2A9 protein in transfected cells. The secreted HIS-tagged 84P2A9 in the culture media can be purified, N 10 using a nickel column using standard techniques.

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SModifications of 84P2A9-related proteins such as covalent modifications are C included within the scope of this invention. One type of covalent modification includes reacting targeted amino acid residues of an 84P2A9 polypeptide with an organic derivatizing agent that is capable of reacting with selected side chains or the N- or Cterminal residues of the 84P2A9. Another type of covalent modification of the 84P2A9 polypeptide included within the scope of this invention comprises altering the native glycosylation pattern of a protein of the invention. "Altering the native glycosylation pattern" is intended for purposes herein to mean deleting one or more carbohydrate moieties found in native sequence 84P2A9 (either by removing the underlying glycosylation site or by deleting the glycosylation by chemical and/or enzymatic means), and/or adding one or more glycosylation sites that are not present in the native sequence 84P2A9. In addition, the phrase includes qualitative changes in the glycosylation of the native proteins, involving a change in the nature and proportions of the various carbohydrate moieties present. Another type of covalent modification of 84P2A9 comprises linking the 84P2A9 polypeptide to one of a variety of nonproteinaceous polymers, polyethylene glycol (PEG), polypropylene glycol, or polyoxyalkylenes, in the manner set forth in U.S. Patent Nos. 4,640,835; 4,496,689; 4,301,144; 4,670,417; 4,791,192 or 4,179,337.

The 84P2A9 of the present invention can also be modified in a way to form a chimeric molecule comprising 84P2A9 fused to another, heterologous polypeptide or amino acid sequence. Such a chimeric molecule can be synthesized chemically or

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IN recombinantly. A chimeric molecule can have a protein of the invention fused to O another tumor-associated antigen or fragment thereof, or can comprise fusion of U fragments of the 84P2A9 sequence (amino or nucleic acid) such that a molecule is Screated that is not, through its length, directly homologous to the amino or nucleic acid sequences respectively of FIG. 2 (SEQ ID NO: such a chimeric molecule can comprise multiples of the same subsequence of 84P2A9. A chimeric molecule can comprise a fusion of an 84P2A9-related protein with a polyhistidine epitope tag, which provides an epitope to which immobilized nickel can selectively bind. The epitope tag is generally placed at the amino- or carboxyl- terminus of the 84P2A9. In an alternative 10 embodiment, the chimeric molecule can comprise a fusion of an 84P2A9-related protein O with an immunoglobulin or a particular region of an immunoglobulin. For a bivalent C form of the chimeric molecule (also referred to as an "'immunoadhesin"), such a fusion could be to the Fc region of an IgG molecule. The Ig fusions preferably include the substitution of a soluble (transmembrane domain deleted or inactivated) form of an 84P2A9 polypeptide in place of at least one variable region within an Ig molecule. In a particularly preferred embodiment, the immunoglobulin fusion includes the hinge, CH2 and CH3, or the hinge, CHI, CH2 and CH3 regions of an IgGI molecule. For the production of immunoglobulin fusions see also US Patent No. 5,428,130 issued June 27, 1995.

84P2A9 ANTIBODIES Another aspect of the invention provides antibodies that bind to 84P2A9-related proteins and polypeptides. Preferred antibodies specifically bind to an 84P2A9-related protein and will not bind (or will bind weakly) to non-84P2A9 proteins. In another embodiment, antibodies bind 84P2A9-related proteins as well as the homologs thereof.

84P2A9 antibodies of the invention are particularly useful in prostate cancer diagnostic and prognostic assays, and imaging methodologies. Similarly, such antibodies are useful in the treatment, diagnosis, and/or prognosis of other cancers, to the extent 84P2A9 is also expressed or overexpressed in other types of cancer. Moreover, intracellularly expressed antibodies single chain antibodies) are therapeutically useful

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in treating cancers in which the expression of 84P2A9 is involved, such as for example Sadvanced and metastatic prostate cancers.

U The invention also provides various immunological assays useful for the detection Sand quantification of 84P2A9 and mutant 84P2A9-related proteins. Such assays can comprise one or more 84P2A9 antibodies capable of recognizing and binding an 84PZA9 or mutant 84P2A9 protein, as appropriate, and are performed within various immunological assay formats well known in the art, including but not limited to various types of radioimmunoassays, enzyme-linked immunosorbent assays (ELISA), enzymelinked immunofluorescent assays (ELIFA), and the like.

S 10 Related immunological but non-antibody assays of the invention also comprise T Scell immunogenicity assays (inhibitory or stimulatory) as well as major histocompatibility C complex (MHC) binding assays. In addition, immunological imaging methods capable of detecting prostate cancer and other cancers expressing 84P2A9 are also provided by the invention, including but limited to radioscintigraphic imaging methods using labeled 84P2A9 antibodies. Such assays are clinically useful in the detection, monitoring, and prognosis of 84P2A9 expressing cancers such as prostate cancer.

84P2A9 antibodies can also be used in methods for purifying 84P2A9 and mutant 84P2A9 proteins and polypeptides and for isolating 84P2A9 homologues and related molecules. For example, in one embodiment, the method of purifying an 84P2A9 protein comprises incubating an 84P2A9 antibody, which has been coupled to a solid matrix, with a lysate or other solution containng 84P2A9 under conditions that permit the 84P2A9 antibody to bind to 84P2A9; washing the solid matrix to eliminate impurities; and eluting the 84P2A9 from the coupled antibody. Other uses of the 84P2A9 antibodies of the invention include generating anti-idiotypic antibodies that mimic the 84P2A9 protein.

Various methods for the preparation of antibodies are well known in the art. For example, antibodies can be prepared by immunizing a suitable mammalian host using an 84P2A9-related protein, peptide, or fragment, in isolated or immunoconjugated form (Antibodies: A Laboratory Manual, CSH Press, Eds., Harlow, and Lane (1988); Harlow, Antibodies, Cold Spring Harbor Press, NY (1989)). In addition, fusion proteins of 84P2A9 can also be used, such as an 84P2A9 GST-fusion protein. In a particular embodiment, a GST fusion protein comprising all or most of the open reading frame amino acid sequence of FIG. 2 is produced and used as an immunogen to generate appropriate antibodies. In Sanother embodiment, an 84P2A9 peptide is synthesized and used as an immunogen.

SIn addition, naked DNA immunization techniques known in the art are used (with Sor without purified 84P2A9 protein or 84P2A9 expressing cells) to generate an immune response to the encoded immunogen (for review, see Donnelly et al., 1997, Ann. Rev.

Immunol. 15: 617-648).

The amino acid sequence of 84P2A9 as shown in FIG. 2 can be used to select specific regions of the 84P2A9 protein for generating antibodies. For example, hydrophobicity and hydrophilicity analyses of the 84P2A9 amino acid sequence are used to C 10 identify hydrophilic regions in the 84P2A9 structure. Regions of the 84P2A9 protein that Sshow immunogenic structure, as well as other regions and domains, can readily be C1 identified using various other methods known in the art, such as Chou-Fasman, Garnier- Robson, Kyte-Doolittle, Eisenberg, Karplus-Schultz or Jameson-Wolf analysis. Thus, each region identified by any of these programs/methods is within the scope of the present invention. Methods for the generation of 84P2A9 antibodies are further illustrated by way of the examples provided herein.

Methods for preparing a protein or polypeptide for use as an immunogen and for preparing immunogenic conjugates of a protein with a carrier such as BSA, KLH, or other carrier proteins are well known in the art. In some circumstances, direct conjugation using, for example, carbodiimide reagents are used; in other instances linking reagents such as those supplied by Pierce Chemical Co., Rockford, IL, are effective. Administration of an 84P2A9 immunogen is conducted generally by injection over a suitable tme period and with use of a suitable adjuvant, as is generally understood in the art. During the immunization schedule, titers of antibodies can be taken to determine adequacy of antibody formation.

84P2A9 monoclonal antibodies can be produced by various means well known in the art. For example, immortalized cell lines that secrete a desired monoclonal antibody are prepared using the standard hybridoma technology of Kohler and Milstein or modifications that immortalize producing B cells, as is generally known. The immortalized cell lines that secrete the desired antibodies are screened by immunoassay in which the antigen is an 84P2A9-related protein. When the appropriate immortalized cell culture secreting the

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Sdesired antibody is identified, the cells can be expanded and antibodies produced either Sfrom in vitro cultures or from ascites fluid.

STlhe antibodies or fragments can also be produced, using current technology, by Srecombinant means. Regions that bind specifically to the desired regions of the 84P2A9 protein can also be produced in the context of chimeric or complementarity determining region (CDR) grafted antibodies of multiple species origin. Humanized or human 84P2A9 antibodies can also be produced and are preferred for use in therapeutic contexts. Methods Sfor humanizing murine and other non-human antibodies, by substituting one or more of the non-human antibody CDRs for corresponding human antibody sequences, are well 10 known (see for example, Jones et al., 1986, Nature 321: 522-525; Riechmnan et al., 1988, SNature 332: 323-327; Verhoeyen et al., 1988, Science 239: 1534-1536). See also, Carter et C1 al., 1993, Proc. Natl. Acad. Sci. USA 89: 4285 and Sims et al., 1993, J. Immunol. 151: 2296.

Methods for producing fully human monoclonal antibodies include phage display and transgenic methods (for review, see Vaughan et al., 1998, Nature Biotechnology 16: 535- 539).

Fully human 84P2A9 monoclonal antibodies can be generated using cloning technologies employing large human Ig gene combinatorial libraries phage display) (Griffiths and Hoogenboom, Building an in vitro immune system: human antibodies from phage display libraries. In: Protein Engineering of Antibody Molecules for Prophylactic and Therapeutic Applications in Man. Clark, M. Nottingham Academic, pp 45-64 (1993); Burton and Barbas, Human Antibodies from combinatorial libraries. Id., pp 65-82).

Fully human 84P2A9 monoclonal antibodies can also be produced using transgenic mice engineered to contain human immunoglobulin gene loci as described in PCT Patent Application W098/24893, Kucherlapati and Jakobovits et al., published December 3, 1997 (see also, Jakobovits, 1998, Exp. Opin. Invest. Drugs 607-614). This method avoids the in vitro manipulation required with phage display technology and efficiently produces high affinity authentic human antibodies.

Reactivity of 84P2A9 antibodies with an 84P2A9-related protein can be established by a number of well known means, including Western blot, immunoprecipitation, ELISA, and FACS analyses using, as appropriate, 84P2A9-related proteins, peptides, 84P2A9-expressing cells or extracts thereof.

J

NO

An 84P2A9 antibody or fragment thereof of the invention is labeled with a Sdetectable marker or conjugated to a second molecule. Suitable detectable markers U include, but are not limited to, a radioisotope, a fluorescent compound, a bioluminescent C compound, chemiluminescent compound, a metal chelator or an enzyme. Further, bispecific antibodies specific for two or more 84P2A9 epitopes are generated using methods generally known in the art. Homodimeric antibodies can also be generated by cross-linking techniques known in the art Wolff et al., Cancer Res. 53: 2560-2565).

84P2A9 TRANSGENIC ANIMALS \Q 10 Nucleic acids that encode 84P2A9 or its modified forms can also be used to Sgenerate either transgenic animals or "knock out" animals which, in turn, are useful in the development and screening of therapeutically useful reagents. In accordance with established techniques, cDNA encoding 84P2A9 can be used to clone genomic DNA encoding 84P2A9 and the genomic sequences used to generate transgenic animals that contain cells that express DNA encoding 84P2A9. Methods for generating transgenic animals, particularly animals such as mice or rats, have become conventional in the art and are described, for example, in U.S. Patent Nos. 4,736,866 and 4,870,009. Typically, particular cells would be targeted for 84P2A9 transgene incorporation with tissue-specific enhancers.

Transgenic animals that include a copy of a transgene encoding 84P2A9 can be used to examine the effect of increased expression of DNA encoding 84P2A9. Such animals can be used as tester animals for reagents thought to confer protection from, for example, pathological conditions associated with its overexpression. In accordance with this facet of the invention, an animal is treated with a reagent and a reduced incidence of the pathological condition, compared to untreated animals bearing the transgene, would indicate a potential therapeutic intervention for the pathological condition.

Alternatively, non-human homologues of 84P2A9 can be used to construct an 84P2A9 "knock out" animal that has a defective or altered gene encoding 84P2A9 as a result of homologous recombination between the endogenous gene encoding 84P2A9 and altered genomic DNA encoding 84P2A9 introduced into an embryonic cell of the animal. For example, cDNA encoding 84P2A9 can be used to clone genomic DNA

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I encoding 84P2A9 in accordance with established techniques. A portion of the genomic SDNA encoding 84P2A9 can be deleted or replaced with another gene, such as a gene 0encoding a selectable marker that can be used to monitor integration. Typically, several Skilobases of unaltered flanking DNA (both at the 5' and 3' ends) are included in the vector [see, Thomas and Capecchi, Cell, 51:503 (1987) for a description of homologous recombination vectors]. The vector is introduced into an embryonic stem cell line by electroporation) and cells in which the introduced DNA has homologously recombined with the endogenous DNA are selected [see, Li et al., Cell, 69:915 (1992)]. The selected cells are then injected into a blastocyst of an animal 1 10 a mouse or rat) to form aggregation chimeras Isee, Bradley, in Teratocarcinomas Sand Embryonic Stem Cells: A Practical Approach, E. J. Robertson, ed. (IRL, Oxford, 1987), C pp. 113-152]. A chimeric embryo can then be implanted into a suitable pseudopregnant female foster anrmal and the embryo brought to term to create a "knock out" animal.

Progeny harboring the homologously recombined DNA in their germ cells can be identified by standard techniques and used to breed animals in which all cells of the animal contain the homologously recombined DNA. Knock out animals can be characterized for instance, for their ability to defend against certain pathological conditions and for their development of pathological conditions due to absence of the 84P2A9 polypeptide.

METHODS FOR THE DETECTION OF 84P2A9 Another aspect of the present invention relates to methods for detecting 84P2A9 polynucleotides and 84P2A9-related proteins and variants thereof, as well as methods for identifying a cell that expresses 84P2A9. 84P2A9 appears to be expressed in the LAPC xenografts that are derived from lymph-node and bone metastasis of prostate cancer. The expression profile of 84P2A9 makes it a potential diagnostic marker for metastasized disease. In this context, the status of 84P2A9 gene products provide information useful for predicting a variety of factors including susceptibility to advanced stage disease, rate of progression, and/or tumor aggressiveness. As discussed in detail below, the status of 84P2A9 gene products in patient samples can be analyzed by a variety protocols that arc

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well known in the art including immunohistochemical analysis, the variety of Northern blotting techniques including in situ hybridization, RT-PCR analysis (for example on laser 0 capture micro-dissected samples), Western blot analysis and tissue array analysis.

SMore particularly, the invention provides assays for the detection of 84P2A9 5 polynuclcotides in a biological sample, such as serum, bone, prostate, and other tssues, urine, semen, cell preparations, and the like. Detectable 84P2A9 polynucleotides include, for example, an 84P2A9 gene or fragments thereof, 84P2A9 mRNA, alternative splice variant 84P2A9 mRNAs, and recombinant DNA or RNA molecules containing an 84P2A9 polynucleotide. A number of methods for amplifying and/or detecting the presence of 84P2A9 polynucleotides are well known in the art and can be employed in the practice of

NO

Sthis aspect of the invention.

C1 In one embodiment, a method for detecting an 84P2A9 mRNA in a biological sample comprises producing cDNA from the sample by reverse transcription using at least one primer; amplifying the cDNA so produced using an 84P2A9 polynucleotides as sense and antisense primers to amplify 84P2A9 cDNAs therein; and detecting the presence of the amplified 84P2A9 cDNA. Optionally, the sequence of the amplified 84P2A9 cDNA can be determined.

In another embodiment, a method of detecting an 84P2A9 gene in a biological sample comprises first isolating genomic DNA from the sample; amplifying the isolated genomic DNA using 84P2A9 polynucleotides as sense and antisense primers to amplify the 84P2A9 gene therein; and detecting the presence of the amplified 84P2A9 gene. Any number of appropriate sense and antisense probe combinations can be designed from the nucleotide sequences provided for the 84P2A9 (FIG. 2) and used for this purpose.

The invention also provides assays for detecting the presence of an 84P2A9 protein in a tissue of other biological sample such as serum, bone, prostate, and other tissues, urine, cell preparations, and the like. Methods for detecting an 84P2A9 protein are also well known and include, for example, immunoprecipitation, immunohistochemical analysis, Western Blot analysis, molecular binding assays, ELISA, ELIFA and the like. For example, in one embodiment, a method of detecting the presence of an 84P2A9 protein in a biological sample comprises first contacting the sample with an 84P2A9 antibody, an 84P2A9-reactive fragment thereof, or a recombinant protein containing an antigen I binding region of an 84P2A9 antibody; and then detecting the binding of 84P2A9 Sprotein in the sample thereto.

0 Methods for identifying a cell that expresses 84P2A9 are also provided. In one Sembodiment, an assay for identifying a cell that expresses an 84P2A9 gene comprises 1> 5 detecting the presence of 84P2A9 mRNA in the cell. Methods for the detection of particular mRNAs m cells are well known and include, for example, hybridization assays using complementary DNA probes (such as in situ hybridization using labeled 84P2A9 riboprobes, Northern blot and related techniques) and various nucleic acid amplification assays (such as RT-PCR using complementary primers specific for 84P2A9, and other l 10 amplification type detection methods, such as, for example, branched DNA, SISBA, TMA

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Sand the like). Alternatively, an assay for identifying a cell that expresses an 84P2A9 gene C, comprises detecting the presence of 84P2A9 protein in the cell or secreted by the cell.

Various methods for the detection of proteins are well known in the art and are employed for the detection of 84P2A9 proteins and 84P2A9 expressing cells.

84P2A9 expression analysis is also useful as a tool for identifying and evaluating agents that modulate 84P2A9 gene expression. For example, 84P2A9 expression is significantly upregulated in prostate cancer, and is also expressed in other cancers including prostate, testis, kidney, brain, bone, skin, ovarian, breast, pancreas, colon, lymphocytic and lung cancers. Identification of a molecule or biological agent that could inhibit 84P2A9 expression or over-expression in cancer cells is of therapeutic value. For example, such an agent can be identified by using a screen that quantifies 84P2A9 expression by RT-PCR, nucleic acid hybridization or antibody binding.

MONITORING THE STATUS OF 84P2A9 AND ITS PRODUCTS Assays that evaluate the status of the 84P2A9 gene and 84P2A9 gene products in an individual can provide information on the growth or oncogenic potential of a biological sample from this individual. For example, because 84P2A9 mRNA is so highly expressed in prostate cancers (as well as the other cancer tissues shown for example in FIGS. 4-8) as compared to normal prostate tissue, assays that evaluate the relative levels of 84P2A9 mRNA transcripts or proteins in a biological sample can be used to diagnose a disease

NO

associated with 84P2A9 disregulation such as cancer and can provide prognostic Sinformation useful in defining appropriate therapeutic options.

SBecause 84P2A9 is expressed, for example, in various prostate cancer xenograft Stissues and cancer cell lines, and cancer patient samples, the expression status of 84P2A9 can provide information useful for determining information including the presence, stage and location of dysplastic, precancerous and cancerous cells, predicting susceptibility to various stages of disease, and/or for gauging tumor aggressiveness. Moreover, the expression profile makes it useful as an imaging reagent for metastasized disease.

Consequently, an important aspect of the invention is directed to the various molecular prognostic and diagnostic methods for examining the status of 84P2A9 in biological Ssamples such as those from individuals suffering from, or suspected of suffering from a CN pathology characterized by disregulated cellular growth such as cancer.

Oncogenesis is known to be a multistep process where cellular growth becomes progressively disregulated and cells progress from a normal physiological state to precancerous and then cancerous states (see, Alers et al., Lab Invest. 77(5): 437-438 (1997) and Isaacs et al., Cancer Sunr. 23: 19-32 (1995)). In this context, examining a biological sample for evidence of disregulated cell growth (such as aberrant 84P2A9 expression in prostate cancers) can allow the early detection of such aberrant cellular physiology before a pathology such as cancer has progressed to a stage at which therapeutic options are more limited. In such examinations, the status of 84P2A9 in a biological sample of interest (such as one suspected of having disregulated cell growth) can be compared, for example, to the status of 84P2A9 in a corresponding normal sample a sample from that individual (or alternatively another individual) that is not effected by a pathology, for example one not suspected of having disregulated cell growth). Alterations in the status of 84P2A9 in the biological sample of interest (as compared to the normal sample) provides evidence of disregulated cellular growth. In addition to using a biological sample that is not effected by a pathology as a normal sample, one can also use a predetermined normative value such as a predetermined normal level of mRNA expression (see, Grcver et al., J. Comp. Neurol. 1996 Dec 9;376(2):306-14 and U.S. patent No. 5,837,501) to compare 84P2A9 in normal versus suspect samples.

NO

The term "status" in this context is used according to its art accepted meaning and Srefers to the condition or state of a gene and its products. Typically, skilled artisans use a Snumber of parameters to evaluate the condition or state of a gene and its products. These Sinclude, but are not limited to the location of expressed gene products (including the 5 location of 84P2A9 expressing cells) as well as the, level, and biological activity of expressed gene products (such as 84P2A9 mRNA polynucleotides and polypcptides).

Alterations in the status of 84P2A9 can be evaluated by a wide variety of methodologies Swell known in the art, typically those discussed herein. Typically an alteration in the status of 84P2A9 comprises a change in the location of 84P2A9 and/or 84P2A9 expressing cells and/or an increase in 84P2A9 mRNA and/or protein expression.

SAs discussed in detail herein, in order to identify a condition or phenomenon r1 associated with disregulated cell growth, the status of 84P2A9 in a biological sample is evaluated by a number of methods utilzed by skilled artisans including, but not limited to genomic Southern analysis (to examine, for example perturbations in the 84P2A9 gene), Northern analysis and/or PCR analysis of 84P2A9 mRNA (to examine, for example alterations in the polynuclcotide sequences or expression levels of 84P2A9 mRNAs), and Western and/or immunohistochemical analysis (to examine, for example alterations in polypeptide sequences, alterations in polypeptide localization within a sample, alterations in expression levels of 84P2A9 proteins and/or associations of 84P2A9 proteins with polypeptide binding partners). Detectable 84P2A9 polynucleotides include, for example, an 84P2A9 gene or fragments thereof, 84P2A9 mRNA, alternative splice variants 84P2A9 mRNAs, and recombinant DNA or RNA molecules containing an 84P2A9 polynucleotide.

The expression profile of 84P2A9 makes it a diagnostic marker for local and/or metastasized disease. In particular, the status of 84P2A9 provides information useful for predicting susceptibility to particular disease stages, progression, and/or tumor aggressiveness. 1The invention provides methods and assays for determining 84P2A9 status and diagnosing cancers that express 84P2A9, such as cancers of the prostate, bladder, testis, ovaries, breast, pancreas, colon and lung. 84P2A9 status in patient samples can be analyzed by a number of means well known in the art, including without limitation, immunohistochemical analysis, in situ hybridization, RT-PCR analysis on laser capture

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Smcro-dissected samples, Western blot analysis of clinical samples and cell lines, and tissue array analysis. Typical protocols for evaluating the status of the 84P2A9 gene and gene 0products can be found, for example in Ausubul et al. eds., 1995, Current Protocols In SMolecular Biology, Units 2 [Northern Blotting], 4 [Southern Blotting], [mmunoblotting] and 18 [PCR Analysis].

As described above, the status of 84P2A9 in a biological sample can be examined by a number of well known procedures in the art. For example, the status of 84P2A9 in a biological sample taken from a specific location in the body can be examined by evaluating the sample for the presence or absence of 84P2A9 expressing cells those that express 84P2A9 mRNAs or proteins). This examination can provide evidence of Sdisregulated cellular growth, for example, when 84P2A9 expressing cells are found in a C biological sample that does not normally contain such cells (such as a lymph node). Such alterations in the status of 84P2A9 in a biological sample are often associated with disregulated cellular growth. Specifically, one indicator of disregulated cellular growth is the metastases of cancer cells from an organ of origin (such as the tesus or prostate gland) to a different area of the body (such as a lymph node). In this context, evidence of disregulated cellular growth is important for example because occult lymph node metastases can be detected in a substantial proportion of patients with prostate cancer, and such metastases are associated with known predictors of disease progression (see, Murphy et al., Prostate 42(4): 315-317 (2000);Su et al., Semin. Surg. Oncol. 18(1): 17- 28 (2000) and Freeman et al.,J Urol 1995 Aug;154(2 Pt 1):474-8).

In one aspect, the invention provides methods for monitoring 84P2A9 gene products by determining the status of 84P2A9 gene products expressed by cells in a test tissue sample from an individual suspected of having a disease associated with disregulated cell growth (such as hyperplasia or cancer) and then comparing the status so determined to the status of 84P2A9 gene products in a corresponding normal sample, the presence of aberrant 84P2A9 gene products in the test sample relative to the normal sample providing an indication of the presence of disregulated cell growth within the cells of the individual.

In another aspect, the invention provides assays useful in determining the presence of cancer in an individual, comprising detecting a significant increase in 84P2A9 O mRNA or protein expression in a test cell or tissue sample relative to expression levels in the corresponding normal cell or tissue. The presence of 84P2A9 mRNA can, for 0 example, be evaluated in tissue samples including but not limited to prostate, testis, Skidney, brain, bone, skin, ovarian, breast, pancreas, colon, lymphocytic and lung tissues (see, FIGS. The presence of significant 84P2A9 expression in any of these tissues is useful to indicate the emergence, presence and/or severity of a cancer, since the corresponding normal tissues do not express 84P2A9 mRNA or express it at lower levels.

In a related embodiment, 84P2A9 status is determined at the protein level rather N 10 than at the nucleic acid level. For example, such a method or assay comprises determining

NO

Sthe level of 84P2A9 protein expressed by cells in a test tissue sample and comparing the C1 level so determined to the level of 84P2A9 expressed in a corresponding normal sample.

In one embodiment, the presence of 84P2A9 protein is evaluated, for example, using immunohistochemical methods. 84P2A9 antibodies or binding partners capable of detecting 84P2A9 protein expression are used in a variety of assay formats well known in the art for this purpose.

In other related embodiments, one can evaluate the status 84P2A9 nucleotide and amino acid sequences in a biological sample in order to identify perturbations in the structure of these molecules such as insertions, deletions, substitutions and the like. Such embodiments are useful because perturbations in the nucleotide and amino acid sequences are observed in a large number of proteins associated with a growth disregulated phenotype (see, Marrogi et al., 1999, J. Cutan. Pathol. 26(8):369-378). For example, a mutation is the sequence of 84P2A9 may be indicative of the presence or promotion of a tumor.

Such assays can therefore have diagnostic and predictive value where a mutation in 84P2A9 indicates a potential loss of function or increase in rumor growth.

A wide variety of assays for observing perturbations in nucleotide and amino acid sequences are well known in the art. For example, the size and structure of nucleic acid or amino acid sequences of 84P2A9 gene products are observed by the Northern, Southern, Western, PCR and DNA sequencing protocols discussed herein. In addition, other methods for observing perturbations in nucleotide and amino acid sequences such as single O strand conformation polymorphism analysis are well known in the art (see, U.S. Patent Nos. 5,382,510 and 5,952,170).

0 In another embodiment, one can examine the methyladon status of the 84P2A9 Sgene in a biological sample. Aberrant demethylation and/or hypermethylanon of CpG 1> 5 islands in gene 5' regulatory regions frequently occurs in immortalized and transformed cells and can result in altered expression of various genes. For example, promoter hypermethylation of the pi-class glutathione S-transferase (a protein expressed in normal prostate but not expressed in >90% of prostate carcinomas) appears to permanently silence transcription of this gene and is the most frequently detected genomic alteration C 10 in prostate carcinomas (De Marzo et al., Am. J. Pathol. 155(6): 1985-1992 (1999)). In

NO

Saddition, this alteration is present in at least 70% of cases of high-grade prostatic C1 intraepithelial neoplasia (PIN) (Brooks et al, Cancer Epidemiol. Biomarkers Prev., 1998, 7:531-536). In another example, expression of the LAGE-I tumor specific gene (which is not expressed in normal prostate but is expressed in 25-50% of prostate cancers) is induced by deoxy-azacytidine in lymphoblastoid cells, suggesting that tumoral expression is due to demethylation (Lethe et al., Int. J. Cancer 76(6): 903-908 (1998)). In this context, a variety of assays for examining methylation status of a gene are well known in the art. For example, one can utilize, in Southern hybridization approaches, methylationsensitive restriction enzymes which can not cleave sequences that contain methylated CpG sites, in order to assess the overall methylation status of CpG islands. In addition, MSP (methylation specific PCR) can rapidly profile the methylation status of all the CpG sites present in a CpG island of a given gene. This procedure involves initial modification of DNA by sodium bisulfite (which will convert all unmethylated cytosines to uracil) followed by amplification using primers specific for methylated versus unmethylated DNA.

Protocols involving methylation interference can also be found for example in Current Protocols In Molecular Biology, Units 12, Frederick M. Ausubul et al. cds., 1995.

Gene amplification provides an additional method of assessing the status of 84P2A9, a locus that maps to lq 3 2 3 a region shown to be perturbed in a variety of cancers. Gene amplification is measured in a sample directly, for example, by conventional Southern blotting or Northern blotting to quantitate the transcription of mRNA (Thomas, 1980, Proc. Natl. Acad. Sci. USA, 77:5201-5205), dot blotting (DNA N analysis), or in situ hybridization, using an appropriately labeled probe, based on the Ssequences provided herein. Alternatively, antibodies are employed that recognize specific duplexes, including DNA duplexes, RNA duplexes, and DNA-RNA hybrid Sduplexes or DNA-protein duplexes. The antibodies in turn are labeled and the assay carried out where the duplex is bound to a surface, so that upon the formation of duplex on the surface, the presence of antibody bound to the duplex can be detected.

In addition to the tissues discussed herein, biopsied tissue or peripheral blood can be conveniently assayed for the presence of cancer cells, including but not limited to prostate, testis, kidney, brain, bone, skin, ovarian, breast, pancreas, colon, lymphocytic C1 10 and lung cancers using for example, Northern, dot blot or RT-PCR analysis to detect S84P2A9 expression (see, FIGS The presence of RT-PCR amplifiable 84P2A9 Cn mRNA provides an indication of the presence of the cancer. RT-PCR detection assays for tumor cells in peripheral blood are currently being evaluated for use in the diagnosis and management of a number of human solid tumors. In the prostate cancer field, these include RT-PCR assays for the detection of cells expressing PSA and PSM (Verkaik et al., 1997, Urol. Res. 25:373-384; Ghossein et al., 1995, J. Clin. Oncol. 13:1195-2000; Heston et al., 1995, Clin. Chem. 41:1687-1688). RT-PCR assays are well known in the art.

A related aspect of the invention is directed to predicting susceptibility to developing cancer in an individual. In one embodiment, a method for predicting susceptibility to cancer comprises detecting 84P2A9 mRNA or 84P2A9 protein in a tissue sample, its presence indicating susceptibility to cancer, wherein the degree of 84P2A9 mRNA expression present correlates to the degree of susceptibility. In a specific embodiment, the presence of 84P2A9 in prostate or other tssue is examined, with the presence of 84P2A9 in the sample providing an indication of prostate cancer susceptibility (or the emergence or existence of a prostate tumor). In a closely related embodiment, one can evaluate the integrity 84P2A9 nucleotide and amino acid sequences in a biological sample in order to identify perturbations in the structure of these molecules such as insertions, deletions, substitutions and the like, with the presence of one or more perturbations in 84P2A9 gene products in the sample providing an indication of cancer susceptibility (or the emergence or existence of a tumor).

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N Another related aspect of the invention is directed to methods for gauging tumor C aggressiveness. In one embodiment, a method for gauging aggressiveness of a tumor comprises determining the level of 84P2A9 mRNA or 84P2A9 protein expressed by cells in C a sample of the tumor, comparing the level so determined to the level of 84P2A9 mRNA or 84P2A9 protein expressed in a corresponding normal tissue taken from the same individual or a normal tissue reference sample, wherein the degree of 84P2A9 mRNA or 84P2A9 protein expression in the tumor sample relative to the normal sample indicates the F degree of aggressiveness. In a specific embodiment, aggressiveness of a tumor is evaluated by determining the extent to which 84P2A9 is expressed in the tumor cells, with higher expression levels indicating more aggressive tumors. In a closely related embodiment, one can evaluate the integrity of 84P2A9 nucleotide and amino acid sequences in a biological Ssample in order to identify perturbations in the structure of these molecules such as insertions, deletions, substitutions and the like, with the presence of one or more perturbations indicating more aggressive tumors.

Yet another related aspect of the invention is directed to methods for observing the progression of a malignancy in an individual over tme. In one embodiment, methods for observing the progression of a malignancy in an individual over time comprise determining the level of 84P2A9 mRNA or 84P2A9 protein expressed by cells in a sample of the tumor, comparing the level so determined to the level of 84P2A9 mRNA or 84P2A9 protein expressed in an equivalent tissue sample taken from the same individual at a different time, wherein the degree of 84P2A9 mRNA or 84P2A9 protein expression in the tumor sample over time provides information on the progression of the cancer. In a specific embodiment, the progression of a cancer is evaluated by determining the extent to which 84P2A9 expression in the rumor cells alters over time, with higher expression levels indicating a progression of the cancer. Also, one can evaluate the integrity 84P2A9 nucleotide and amino acid sequences in a biological sample in order to identify perturbations in the structure of these molecules such as insertions, deletions, substitutions and the like, with the presence of one or more perturbations indicating a progression of the cancer.

The above diagnostic approaches can be combined with any one of a wide variety of prognostic and diagnostic protocols known in the art. For example, another

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N embodiment of the invention disclosed herein is directed to methods for observing a Scoincidence between the expression of 84P2A9 gene and 84P2A9 gene products (or U perturbations in 84P2A9 gene and 84P2A9 gene products) and a factor that is associated with malignancy as a means of diagnosing and prognosticating the status of a tissuc sample.

In this context, a wide variety of factors associated with malignancy can be utilized such as the expression of genes associated with malignancy PSA, PSCA and PSM expression for prostate cancer etc.) as well as gross cytological observations (see, Bocking et al., 1984, Anal. Quant. Cytol. 6(2):74-88; Eptsein, 1995, Hum. Pathol. 26(2):223-9; Thorson et al., 1998, Mod. Pathol. 11(6):543-51; Baisden et al., 1999, Am. J. Surg. Pathol.

23(8):918-24). Methods for observing a coincidence between the expression of 84P2A9 Sgene and 84P2A9 gene products (or perturbations in 84P2A9 gene and 84P2A9 gene C products) and an additional factor that is associated with malignancy are useful, for example, because the presence of a set of specific factors that coincide with disease provides information crucial for diagnosing and prognosticating the status of a tissue sample.

In a typical embodiment, methods for observing a coincidence between the expression of 84P2A9 gene and 84P2A9 gene products (or perturbations in 84P2A9 gene and 84P2A9 gene products) and a factor that is associated with malignancy entails detecting the overexpression of 84P2A9 mRNA or protein in a tissue sample, detecting the overexpression of PSA mRNA or protein in a tissue sample, and observing a coincidence of 84P2A9 mRNA or protein and PSA mRNA or protein overexpression. In a specific embodiment, the expression of 84P2A9 and PSA mRNA in prostate tissue is examined. In a preferred embodiment, the coincidence of 84P2A9 and PSA mRNA overexpression in the sample provides an indication of prostate cancer, prostate cancer susceptibility or the emergence or status of a prostate tumor.

Methods for detecting and quantifying the expression of 84P2A9 mRNA or protein are described herein and use of standard nucleic acid and protein detection and quantification technologies is well known in the art. Standard methods for the detection and quantification of 84P2A9 mRNA include in situ hybridization using labeled 84P2A9 rboprobes, Northern blot and related techniques using 84P2A9 polynucleotde probes, RT-PCR analysis using primers specific for 84P2A9, and other amplification type detection IN methods, such as, for example, branched DNA, SISBA, TMA and the like. In a specific O" embodiment, semi-quantitative RT-PCR is used to detect and quantify 84P2A9 mRNA expression as described in the Examples that follow. Any number of primers capable of Samplifying 84P2A9 can be used for this purpose, including but not limited to the various 5 primer sets specifically described herein. Standard methods for the detection and quantification of protein are used for this purpose. In a specific embodiment, polyclonal or monoclonal antibodies specifically reactive with the wild-type 84P2A9 protein can be used in an immunohistochemical assay of biopsied tissue.

0 10 IDENTIFYING MOLECULES THAT INTERACT WITH 84P2A9

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SThe 84P2A9 protein sequences disclosed herein allow the skilled artisan to identify proteins, small molecules and other agents that interact with 84P2A9 and pathways activated by 84P2A9 via any one of a variety of art accepted protocols. For example, one can utilize one of the variety of so-called interaction trap systems (also referred to as the "two-hybrid assay"). In such systems, molecules that interact reconstitute a transcription factor which directs expression of a reporter gene, whereupon the expression of the reporter gene is assayed. Typical systems identify protein-protein interactions in vivo through reconstitution of a eukaryotic transcriptional activator and are disclosed for example in U.S. Patent Nos. 5,955,280, 5,925,523, 5,846,722 and 6,004,746.

Alternatively one can identify molecules that interact with 84P2A9 protein sequences by screening peptide libraries. In such methods, peptides that bind to selected receptor molecules such as 84P2A9 are identified by screening libraries that encode a random or controlled collection of amino acids. Peptides encoded by the libraries are expressed as fusion proteins of bacteriophage coat proteins, the bacteriophage particles are then screened against the receptors of interest.

Accordingly, peptides having a wide variety of uses, such as therapeutic or diagnostic reagents, can thus be identified without any prior information on the structure of the expected ligand or receptor molecule. Typical peptide libraries and screening

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I methods that can be used to identify molecules that interact with 84P2A9 protein sequences arc disclosed for example in U.S. Patent Nos. 5,723,286 and 5,733,731.

Alternatively, cell lines that express 84P2A9 are used to identify protein-protein Sinteractions mediated by 84P2A9. Such interactions can be examined using 1> 5 immunoprecipitation techniques as shown by others (Hamilton BJ, et al. Biochem.

Biophys. Res. Commun. 1999, 261:646-51). Typically 84P2A9 protein can be immunoprecipitated from 84P2A9 expressing prostate cancer cell lines using anti- 84P2A9 antibodies. Alternatively, antibodies against His-tag can be used in a cell line engineered to express 84P2A9 (vectors mentioned above). The immunoprecipitated C 10 complex can be examined for protein association by procedures such as Western Sblotting, 35 S-methionine labeling of proteins, protein microsequencing, silver staining and CI two dimensional gel clectrophoresis.

Small molecules that interact with 84P2A9 can be identified through related embodiments of such screening assays. For example, small molecules can be identified that interfere with protein function, including molecules that interfere with 84P2A9's ability to mediate phosphorylation and de-phosphorylation, second messenger signaling and tumorigenesis. Typical methods are discussed for example in U.S. Patent No.

5,928,868 and include methods for forming hybrid ligands in which at least one ligand is a small molecule. In an illustrative embodiment, the hybrid ligand is introduced into cells that in turn contain a first and a second expression vector. Each expression vector includes DNA for expressing a hybrid protein that encodes a target protein linked to a coding sequence for a transcriptional module. The cells further contain a reporter gene, the expression of which is conditioned on the proximity of the first and second hybrid proteins to each other, an event that occurs only if the hybrid ligand binds to target sites on both hybrid proteins. Those cells that express the reporter gene are selected and the unknown small molecule or the unknown hybrid protein is identified.

A typical embodiment of this invention consists of a method of screening for a molecule that interacts with an 84P2A9 amino acid sequence shown in FIG. 1 (SEQ ID NO: comprising the steps of contacting a population of molecules with the 84P2A9 amino acid sequence, allowing the population of molecules and the 84P2A9 amino acid sequence to interact under conditions that facilitate an interaction, determinung the

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O presence of a molecule that interacts with the 84P2A9 amino acid sequence and then Sseparating molecules that do not interact with the 84P2A9 amino acid sequence from t molecules that do interact with the 84P2A9 amino acid sequence. In a specific Sembodiment, the method further includes purifying a molecule that interacts with the 84P2A9 amino acid sequence. In a preferred embodiment, the 84P2A9 amino acid sequence is contacted with a library of peptides.

THERAPEUTIC METHODS AND COMPOSITIONS The identification of 84P2A9 as a protein that is normally prostate and testis- C related and which is also expressed in cancers of the prostate (and other cancers), opens N 10 a number of therapeutic approaches to the treatment of such cancers. As discussed Sherein, it is possible that 84P2A9 functions as a transcription factor involved in activating tumor-promoting genes or repressing genes that block tumorigenesis.

The expression profile of 84P2A9 is reminiscent of the Cancer-Testis (CT) antigens or MAGE antigens, which are testis-related genes that are up-regulated in melanomas and other cancers (Van den Eynde and Boon, Int J Clin Lab Res. 27:81-86, 1997). Due to their tissue-specific expression and high expression levels in cancer, the MAGE antigens are currently being investigated as targets for cancer vaccines (Durrant, Anticancer Drugs 8:727-733, 1997; Reynolds et al., Int J Cancer 72:972-976, 1997). The expression pattern of 84P2A9 provides evidence that it is likewise an ideal target for a cancer vaccine approach to prostate cancer. Its structural features indicate that it may be a transcription factor, and provide evidence that 84P2A9 is a small molecule target.

Accordingly, therapeutic approaches aimed at inhibiting the activity of the 84P2A9 protein are expected to be useful for patients suffering from prostate cancer, testicular cancer, and other cancers expressing 84P2A9. These therapeutic approaches generally fall into two classes. One class comprises various methods for inhibiting the binding or association of the 84P2A9 protein with its binding partner or with others proteins. Another class comprises a variety of methods for inhibiting the transcription of the 84P2A9 gene or translation of 84P2A9 mRNA.

84P2A9 as a Target for Antibody-Based Therapy

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N The structural features of 84P2A9 indicate that this molecule is an attractive target for antibody-based therapeutic strategies. A number of typical antibody strategies U are known in the art for targeting both extracellular and intracellular molecules (see, e.g., Scomplement and ADCC mediated killing as well as the use of intrabodies discussed herein). Because 84P2A9 is expressed by cancer cells of various lineages and not by corresponding normal cells, systemic administration of 84P2A9-immunoreactive compositions would be expected to exhibit excellent sensitivity without toxic, non- 1 specific and/or non-target effects caused by binding of the immunotherapeutic molecule to non-target organs and tissues. Antibodies specifically reactive with domains of 84P2A9 can be useful to treat 84P2A9-expressing cancers systemically, either as Sconjugates with a toxin or therapeutic agent, or as naked antibodies capable of inhibiting cell proliferation or function.

84P2A9 antibodies can be introduced into a patient such that the antibody binds to 84P2A9 and modulates or perturbs a function such as an interaction with a binding partner and consequently mediates growth inhibition and/or destruction of the tumor cells and/or inhibits the growth of the tumor cells. Mechanisms by which such antibodies exert a therapeutic effect can include complement-mediated cytolysis, antibody-dependent cellular cytotoxicity, modulating the physiological function of 84P2A9, inhibiting ligand binding or signal transduction pathways, modulating tumor cell differentiation, altering tumor angiogenesis factor profiles, and/or by inducing apoptosis.

Those skilled in the art understand that antibodies can be used to specifically target and bind immunogenic molecules such as an immunogenic region of the 84P2A9 sequence shown in Figure 1. In addition, skilled artisans understand that it is routine to conjugate antibodies to cytotoxic agents. In this context, skilled artisans understand that when cytotoxic and/or therapeutic agents are delivered directy to cells by conjugating them to antibodies specific for a molecule expressed by that cell 84P2A9), it is reasonable to expect that the cytotoxic agent will exert its known biological effect (e.g.

cytotoxicity) on those cells.

A wide variety of compositions and methods for using antibodies conjugated to cytotoxic agents to kill cells are known m the art. In the context of cancers, typical methods entail administering to an animal having a tumor a biologically effective amount

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0 of a conjugate comprising a selected cytotoxic and/or therapeutic agent linked to a 0 targeting agent an anti-84P2A9 antibody) that binds to a marker 84P2A9) expressed, accessible to binding or localized on the cell surfaces. A typical embodiment Sconsists of a method of delivering a cytotoxic and/or therapeutic agent to a cell expressing 84P2A9 comprising conjugating the cytotoxic agent to an antibody that immunospecifically binds to an 84P2A9 epitope and exposing the cell to the antibodyagent conjugate. Another specific illustrative embodiment consists of a method of Streating an individual suspected of suffering from metastasized cancer comprising the step of administering parenterally to said individual a pharmaceutical composition 10 comprising a therapeutically effective amount of an antibody conjugated to a cytotoxic O and/or therapeutic agent.

Cancer immunotherapy using anti-84P2A9 antibodies may follow the teachings generated from various approaches that have been successfully employed in the treatment of other types of cancer, including but not limited to colon cancer (Arlen et al., 1998, Crit. Rev. Immunol. 18:133-138), multiple myeloma (Ozaki et al., 1997, Blood 90:3179-3186; Tsunenari et al., 1997, Blood 90:2437-2444), gastric cancer (Kasprzyk et al., 1992, Cancer Res. 52:2771-2776), B-cell lymphoma (Funakoshi et al., 1996, J.

Immunother. Emphasis Tumor Immunol. 19:93-101), leukerma (Zhong et al., 1996, Leuk. Res. 20:581-589), colorectal cancer (Moun ct al., 1994, Cancer Res. 54:6160-6166; Velders et al., 1995, Cancer Res. 55:4398-4403), and breast cancer (Shepard et al., 1991, J.

Clin. Immunol. 11:117-127). Some therapeutic approaches involve conjugation of naked antibody to a toxin, such as the conjugation of 131I to anti-CD20 antibodies Rituxan

M

IDEC Pharmaceuticals Corp.), while others involve co-administration of antibodies and other therapeutic agents, such as Herceptin T M (trastuzumab) with paclitaxel (Genentech, Inc.). For treatment of prostate cancer, for example, 84P2A9 antibodies can be administered in conjunction with radiation, chemotherapy or hormone ablation.

Although 84P2A9 antibody therapy is useful for all stages of cancer, antibody therapy is particularly appropriate in advanced or metastatic cancers. Treatment with the antibody therapy of the invention is indicated for patients who have received one or more rounds of chemotherapy, while combining the antibody therapy of the invention with a chemotherapeutic or radiation regimen is preferred for patients who have not Sreceived chemotherapeutic treatment. Additionally, antibody therapy can enable the use O of reduced dosages of concomitant chemotherapy, particularly for patients who do not Stolerate the toxicity of the chemotherapeutic agent very well.

It is desirable for some cancer patients to be evaluated for the presence and level of 84P2A9 expression, preferably using immunohistochemical assessments of tumor tissue, quantitative 84P2A9 imaging, or other techniques capable of reliably indicating the presence and degree of 84P2A9 expression. Immunohistochermcal analysis of tumor biopsies or surgical specimens is preferred for this purpose. Methods for Cl 10 immunohistochemical analysis of tumor tissues are well known in the art.

SAnti-84P2A9 monoclonal antibodies useful in treating prostate and other cancers C1 include those that are capable of initiating a potent immune response against the tumor or those that are directly cytotoxic. In this regard, anti-84P2A9 monoclonal antibodies (mAbs) can elicit tumor cell lysis by either complement-mediated or antibody-dependent cell cytotoxicity (ADCC) mechanisms, both of which require an intact Fc portion of the immunoglobulin molecule for interaction with effector cell Fc receptor sites or complement proteins. In addition, anti-84P2A9 mAbs that exert a direct biological effect on tumor growth are useful in the practice of the invention. Potential mechanisms by which such directly cytotoxic mAbs can act include inhibition of cell growth, modulation of cellular differentiation, modulation of tumor angiogenesis factor profiles, and the induction of apoptosis. The mechanism by which a particular anti-84P2A9 mAb exerts an anti-tumor effect is evaluated using any number of in vitro assays designed to determine cell death such as ADCC, ADMMC, complement-mediated cell lysis, and so forth, as is generally known in the art.

The use of murine or other non-human monoclonal antibodies, or human/mouse chimeric mAbs can induce moderate to strong immune responses in some patients. In some cases, this will result in clearance of the antibody from circulation and reduced efficacy. In the most severe cases, such an immune response can lead to the extensive formation of immune complexes which, potentially, can cause renal failure. Accordingly, preferred monoclonal antibodies used in the practice of the therapeutic methods of the invention are those that are either fully human or humanized O and that bind specifically to the target 84P2A9 antigen with high affinity but exhibit low or no antigenicity in the patient.

Therapeutic methods of the invention contemplate the administration of single C) anti-84P2A9 rnAbs as well as combinations, or cocktails, of different mnAbs. Such mAb cocktails can have certain advantages inasmuch as they contain mAbs that target different epitopes, exploit different effector mechanisms or combine directly cytotoxic mArbs with mAbs that rely on nmmune effector functionality. Such mAbs in combination can exhibit synergistic therapeutic effects. In addition, the administration of anti-84P2A9 mAbs can be combined with other therapeutic agents, including but not limited to various chemotherapeutic agents, androgen-blockers, and immune modulators

NO

II-2, GM-CSF). The anti-84P2A9 mAbs are administered in their "naked" or CK1 unconjugated form, or can have therapeutic agents conjugated to them.

The anti-84P2A9 antibody formulations are administered via any route capable of delivering the antibodies to the tumor site. Potentially effective routes of administration include, but are not limited to, intravenous, intraperitoneal, intramuscular, intratumor, intradermal, and the like. Treatment will generally involve the repeated administration of the anti-84P2A9 antibody preparation via an acceptable route of administration such as intravenous injection typically at a dose in the range of about 0.1 to about mg/kg body weight. Doses in the range of 10-500 mg mAb per week are effectve and well tolerated.

Based on clinical experience with the Herceptin mAb in the treatment of metastatic breast cancer, an initial loading dose of approximately 4 mg/kg patient body weight IV followed by weekly doses of about 2 mg/kg IV of the anti- 84P2A9 mAb preparation represents an acceptable dosing regimen. Preferably, the initial loading dose is administered as a 90 minute or longer infusion. The periodic maintenance dose is administered as a 30 minute or longer infusion, provided the initial dose was well tolerated. However, as one of skill in the art will understand, various factors will influence the ideal dose regimen in a particular case. Such factors can include, for example, the binding affinity and half life of the Ab or mAbs used, the degree of 84P2A9 expression in the patient, the extent of circulating shed 84P2A9 antigen, the desired steady-state antibody concentration level, frequency of treatment, and the influence of Schemotherapeutic agents used in combination with the treatment method of the Sinvention, as well as the health status of a particular patient.

0 Optionally, patients should be evaluated for the levels of 84P2A9 in a given Ssample the levels of circulating 84P2A9 antigen and/or 84P2A9 expressing cells) in order to assist in the deternmnation of the most effective dosing regimen and related factors. Such evaluations are also be used for moitoring purposes throughout therapy, and are useful to gauge therapeutic success in combination with evaluating other parameters (such as serum PSA levels in prostate cancer therapy).

Inhibition of 84P2A9 Protein Function Within the first class of therapeutic approaches, the invention includes various methods and compositions for inhibiting the binding of 84P2A9 to its binding partner or its association with other protein(s) as well as methods for inhibiting 84P2A9 function.

Inhibition of 84P2A9 With Intracellular Antibodies In one approach, recombinant vectors encoding single chain antibodies that specifically bind to 84P2A9 are introduced into 84P2A9 expressing cells via gene transfer technologies, wherein the encoded single chain anti-84P2A9 antibody is expressed intracellularly, binds to 84P2A9 protein, and thereby inhibits its function. Methods for engineering such intracellular single chain antibodies are well known. Such intracellular antibodies, also known as "intrabodies", are specifically targeted to a particular compartment within the cell, providing control over where the inhibitory activity of the treatment will be focused. This technology has been successfully applied in the art (for review, see Richardson and Marasco, 1995, TIBTECH vol. 13). Intrabodies have been shown to virtually eliminate the expression of otherwise abundant cell surface receptors.

See, for example, Richardson et al., 1995, Proc. Natl. Acad. Sci. USA 92: 3137-3141; Beerli et al., 1994, J. Biol. Chem. 289: 23931-23936; Dcshane et al., 1994, Gene Ther. 1: 332-337.

Single chain antibodies comprise the variable domains of the heavy and light chain joined by a flexible linker polypeptide, and are expressed as a single polypeptide.

D Optionally, single chain antibodies are expressed as a single chain variable region Sfragment joined to the light chain constant region. Well known intracellular trafficking 0 signals are engineered into recombinant polynucleotide vectors encoding such single Schain antibodies in order to precisely target the expressed intrabody to the desired 5 intracellular compartment. For example, intrabodies targeted to the endoplasmic reticulum (ER) are engineered to incorporate a leader peptide and, optionally, a Cterminal ER retention signal, such as the KDEL amino acid motif. Intrabodies intended to exert activity in the nucleus are engineered to include a nuclear localization signal.

Lipid moieties are joined to intrabodies in order to tether the intrabody to the cytosolic l 10 side of the plasma membrane. Intrabodies can also be targeted to exert function in the

LO

Scytosol. For example, cytosolic intrabodies are used to sequester factors within the C1 cytosol, thereby preventing them from being transported to their natural cellular destination.

In one embodiment, intrabodies are used to capture 84P2A9 in the nucleus, thereby preventing its activity within the nucleus. Nuclear targeting signals are engineered into such 84P2A9 intrabodies in order to achieve the desired targeting. Such 84P2A9 intrabodies are designed to bind specifically to a particular 84P2A9 domain. In another embodiment, cytosolic intrabodies that specifically bind to the 84P2A9 protein are used to prevent 84P2A9 from gaining access to the nucleus, thereby preventing it from exerting any biological activity within the nucleus preventing 84P2A9 from forming transcription complexes with other factors).

In order to specifically direct the expression of such intrabodies to particular cells, the transcription of the intrabody is placed under the regulatory control of an appropriate tumor-specific promoter and/or enhancer. In order to target intrabody expression specifically to prostate, for example, the PSA promoter and/or promoter/enhancer can be utilized (See, for example, U.S. Patent No. 5,919,652).

Inhibition of 84P2A9 Wilh Recombinant Proteins In another approach, recombinant molecules that bind to 84P2A9 thereby prevent or inhibit 84P2A9 from accessing/binding to its binding partner(s) or associating I with other protein(s) are used to inhibit 84P2A9 function. Such recombinant molecules Scan, for example, contain the reactive part(s) of an 84P2A9 specific antibody molecule. In 0 a particular embodiment, the 84P2A9 binding domain of an 84P2A9 binding partner is Sengineered into a dimeric fusion protein comprising two 84P2A9 ligand binding domains linked to the Fc portion of a human IgG, such as human IgG1. Such IgG portion can contain, for example, the C 1 2 and Cn3 domains and the hinge region, but not the CH1 domain. Such dimeric fusion proteins are administered in soluble form to patients suffering from a cancer associated with the expression of 84P2A9, including but not limited to prostate and testicular cancers, where the dimeric fusion protein specifically binds to 84P2A9 thereby blocking 84P2A9 interaction with a binding partner. Such dimcnc fusion Sproteins are further combined into multimeric proteins using known antibody linking CN technologies.

Inhibition of 84P2A9 Transcription or Translation Within the second class of therapeutic approaches, the invention provides various methods and compositions for inhibiting the transcription of the 84P2A9 gene.

Similarly, the invention also provides methods and compositions for inhibiting the translation of 84P2A9 mRNA into protein.

In one approach, a method of inhibiting the transcription of the 84P2A9 gene comprises contacting the 84P2A9 gene with an 84P2A9 antisense polynucleotide. In another approach, a method of inhibiting 84P2A9 mRNA translation comprises contacting the 84P2A9 mRNA with an antisense polynucleotide. In another approach, an 84P2A9 specific ribozyme is used to cleave the 84P2A9 message, thereby inhibiting translation. Such antisense and ribozyme based methods can also be directed to the regulatory regions of the 84P2A9 gene, such as the 84P2A9 promoter and/or enhancer elements. Similarly, proteins capable of inhibiting an 84P2A9 gene transcription factor can be used to inhibit 84P2A9 mRNA transcription. The various polynuclcotides and compositions useful in the aforementioned methods have been described above. The use of antisense and ribozyme molecules to inhibit transcription and translation is well known in the art.

I Other factors that inhibit the transcription of 84P2A9 through interfering with S84P2A9 transcriptional activation are also useful for the treatment of cancers expressing O 84P2A9. Similarly, factors that are capable of interfering with 84P2A9 processing are Suseful for the treatment of cancers expressing 84P2A9. Cancer treatment methods 5 utilizing such factors are also within the scope of the invention.

General Considerations for Therapeutic Strategies Gene transfer and gene therapy technologies can be used for delivering therapeutic polynucleotide molecules to tumor cells synthesizing 84P2A9 antisensc, ribozyme, N 10 polvnucleotides encoding intrabodies and other 84P2A9 inhibitory molecules). A number Sof gene therapy approaches are known in the art. Recombinant vectors encoding 84P2A9 antisense polynucleotides, ribozymes, factors capable of interfering with 84P2A9 transcription, and so forth, can be delivered to target tumor cells using such gene therapy approaches.

The above therapeutic approaches can be combined with any one of a wide variety of surgical, chemotherapy or radiation therapy regimens. These therapeutic approaches can enable the use of reduced dosages of chemotherapy and/or less frequent administration, an advantage for all patients and particularly for those that do not tolerate the toxicity of the chemotherapeutic agent well.

The anti-tumor activity of a particular composition antisense, ribozyme, intrabody), or a combination of such compositions, can be evaluated using various in vitro and in vivo assay systems. In vitro assays for evaluating therapeutic potential include cell growth assays, soft agar assays and other assays indicative of tumor promoting activity, binding assays capable of determining the extent to which a therapeutic composition will inhibit the binding of 84P2A9 to a binding partner, etc.

In vivo, the effect of an 84P2A9 therapeutic composition can be evaluated in a suitable animal model. For example, xenogenic prostate cancer models wherein human prostate cancer explants or passaged xenograft tissues are introduced into immune compromised animals, such as nude or SCID mice, are appropriate in relation to prostate cancer and have been described (Klein et al., 1997, Nature Medicine 3: 402-408). For example, PCT Patent Application W098/16628, Sawyers et al., published April 23, 1998, Sdescribes various xenograft models of human prostate cancer capable of recapitulating Sthe development of primary tumors, micrometastasis, and the formation of osteoblastic Smetastases characteristic of late stage disease. Efficacy can be predicted using assays that measure inhibition of tumor formation, tumor regression or metastasis, and the like. See, also, the Examples below.

In vivo assays that evaluate the promotion of apoptosis are useful in evaluating therapeutic compositions. In one embodiment, xenografts from tumor bearing mice treated with the therapeutic composition can be examined for the presence of apoptotic C 10 foci and compared to untreated control xenograft-bearing mice. The extent to which 0 apoptotic foci are found in the tumors of the treated mice provides an indication of the CN therapeutic efficacy of the composition.

The therapeutic compositions used in the practice of the foregoing methods can be formulated into pharmaceutical compositions comprising a carier suitable for the desired delivery method. Suitable carriers include any material that when combined with the therapeutic composition retains the anti-tumor function of the therapeutic composition and is generally non-reactive with the patient's immune system. Examples include, but are not limited to, any of a number of standard pharmaceutical carriers such as sterile phosphate buffered saline solutions, bacteriostatic water, and the like (see, generally, Remington's Pharmaceutical Sciences 1 6 h Edition, A. Osal., Ed., 1980).

Therapeutic formulations can be solubilized and administered via any route capable of delivering the therapeutic composition to the tumor site. Potentially effective routes of administration include, but are not limited to, intravenous, parenteral, intraperitoneal, intramuscular, intratumor, intradermal, intraorgan, orthotopic, and the like. A preferred formulation for intravenous injection comprises the therapeutic composition in a solution of preserved bacteriostatic water, sterile unpreserved water, and/or diluted in polyvinylchloride or polyethylene bags containing 0.9% sterile Sodium Chloride for Injection, USP. Therapeutic protein preparations can be lyophilized and stored as sterile powders, preferably under vacuum, and then reconstituted in bacteriostatic water containing, for example, benzyl alcohol preservative, or in sterile water prior to injection.

O Dosages and administration protocols for the treatment of cancers using the Sforegoing methods will vary with the method and the target cancer, and will generally O depend on a number of other factors appreciated in the art.

CANCER VACCINES As noted above, the expression profile of 84P2A9 shows that it is highly expressed in advanced and metastasized prostate cancer. This expression pattern is reminiscent of the Cancer-Testis (CT) antigens or MAGE antigens, which are testisspecific genes that are up-regulated in melanomas and other cancers (Van den Eynde and Boon, Int J Clin Lab Res. 27:81-86, 1997). Due to their tissue-specific expression and high expression levels in cancer, the MAGE antigens are currently being investigated as targets for cancer vaccines (Durrant, Anticancer Drugs 8:727-733, 1997; Reynolds et al., IntJ Cancer 72:972-976, 1997).

The invention further provides cancer vaccines comprising an 84P2A9-related protein or fragment as well as DNA based vaccines. In view of the expression of 84P2A9, cancer vaccines are effective at specifically preventing and/or treating 84P2A9 expressing cancers without creating non-specific effects on non-target tissues. The use of a tumor antigen in a vaccine for generating humoral and cell-mediated immunity for use in anticancer therapy is well known in the art and has been employed in prostate cancer using human PSMA and rodent PAP immunogens (Hodge et al., 1995, Int. J. Cancer 63:231-237; Fong et al., 1997, J. Immunol. 159:3113-3117).

Such methods can be readily practiced by employing an 84P2A9 protein, or fragment thereof, or an 84P2A9-encoding nucleic acid molecule and recombinant vectors capable of expressing and appropriately presenting the 84P2A9 immunogen (which typically comprises a number of humoral or 'T cell epitopes immunoreacive epitopes). In this context, skilled artisans understand that a wide variety of different vaccine systems for delivery of immunoreactive epitopes are known in the art (see, e.g., Heryln et al., Ann Med 1999 Feb;31(1):66-78; Maruyama ct al., Cancer Immunol Immunother 2000 Jun;49(3):123-32) Briefly, such techniques consists of methods of generating an immune response a humoral and/or cell mediated response) in a I mammal comprising the steps exposing the mammal's immune system to an i unmunoreactive cpitope an epitope of the 84P2A9 protein shown in SEQ ID NO: 0 2) so that the mammal generates an immune response that is specific for that epitope S(e.g. generates antibodies that specifically recognize that epitope). In a preferred method, the 84P2A9 immunogen contains a biological motif. In a highly preferred embodiment, the 84P2A9 immunogen contains one or more amino acid sequences identified using one of the pertinent analytical techniques well known in the art such as the sequences shown in Table 1.

A wide variety of methods for generating an immune response in a mammal are l 10 well known in the art (for example as the first step in the generation of hybridomas).

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SMethods of generating an immune response in a mammal comprise exposing the Cl mammal's immune system to an exogenous immunogenic epitope on a protein the 84P2A9 protein of SEQ ID NO: 2) so that an immune response is generated. A typical embodiment consists of a method for generating an immune response to 84P2A9 in a host, by contacting the host with a sufficient amount of 84P2A9 or a B cell or cytotoxic T-cell eliciting epitope or analog thereof; and at least one periodic interval thereafter contacting the host with additional 84P2A9 or a B cell or cytotoxic T-cell eliciting epitope or analog thereof. A specific embodiment consists of a method of generating an immune response against an 84P2A9 protein or a multepitopic peptide comprising administering 84P2A9 immunogen the 84P2A9 protein or a peptide fragment thereof, an 84P2A9 fusion protein etc.) in a vaccine preparation to humans or animals.

Typically, such vaccine preparations further contain a suitable adjuvant. (see, U.S.

Patent No. 6,146,635). A representative variation on these methods consists of a method of generating an immune response in an individual against an 84P2A9 immunogen comprising administering in vivo to muscle or skin of the individual's body a genetic vaccine facilitator such as one selected from the group consisting of: anionic lipids; saponins; lectins; estrogenic compounds; hydroxylated lower alkyls; dimethyl sulfoxide; and urea; and a DNA molecule that is dissociated from an infectious agent and comprises a DNA sequence that encodes the 84P2A9 immunogen, the DNA sequence operatively linked to regulatory sequences which control the expression of the DNA sequence; wherein the DNA molecule is taken up by cells, the DNA sequence is

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N expressed in the cells and an immune response is generated against the immunogen.

O (see, U.S. Patent No. 5,962,428).

O In an illustrative example of a specific method for generating an immune response, Sviral gene delivery systems are used to deliver an 84P2A9-encoding nucleic acid molecule.

Various viral gene delivery systems that can be used in the practice of this aspect of the invention include, but are not limited to, vaccinia, fowlpox, canarypox, adenovirus, influenza, poliovirus, adeno-associated virus, lentvirus, and sindbus virus (Restifo, 1996, SCurr. Opin. Immunol. 8:658-663). Non-viral delivery systems can also be employed by using naked DNA encoding an 84P2A9 protein or fragment thereof introduced into the C 10 patient intramuscularly or intradermally) to induce an ann-tumor response. In one 0 embodiment, the full-length human 84P2A9 cDNA is employed. In another embodiment, C 84P2A9 nucleic acid molecules encoding specific cytotoxic T lymphocyte (CTL) epitopes can be employed. CTL epitopes can be determined using specific algorithms Epimer, Brown University) to identify peptides within an 84P2A9 protein that are capable of optimally binding to specified HLA alleles.

Various ex vivo strategies can also be employed. One approach involves the use of dendritic cells to present 84P2A9 antigen to a patient's immune system. Dendiiuc cells express MHC class I and II molecules, B7 co-stimulator, and LL-12, and are thus highly specialized antigen presenting cells. In prostate cancer, autologous dendritic cells pulsed with peptides of the prostate-specific membrane antigen (PSMA) are being used in a Phase I clinical trial to stimulate prostate cancer patients' immune systems (Tjoa et al., 1996, Prostate 28:65-69; Murphy et al., 1996, Prostate 29:371-380). Thus, dendritic cells can be used to present 84P2A9 peptides to T cells in the context of MHC class 1 and II molecules. In one embodiment, autologous dendritic cells are pulsed with 84P2A9 peptides capable of binding to MHC class I and/or class II molecules. In another embodiment, dendritic cells are pulsed with the complete 84P2A9 protein. Yet another embodiment involves engineering the overexpression of the 84P2A9 gene in dendritic cells using various implementing vectors known in the art, such as adenovirus (Arthur et al., 1997, Cancer Gene Ther. 4:17-25), retrovirus (Henderson et al., 1996, Cancer Res.

56:3763-3770), lentivrus, adeno-associated virus, DNA transfection (Ribas et al., 1997, Cancer Res. 57:2865-2869), or tumor-derived RNA transfection (Ashley et al., 1997, J.

ki Exp. Med. 186:1177-1182). Cells expressing 84P2A9 can also be engineered to express immune modulators, such as GM-CSF, and used as immunizing agents.

SAnti-idiotypic anti-84P2A9 antibodies can also be used in ant-cancer therapy as a vaccme for inducing an immune response to cells expressing an 84P2A9 protein.

Specifically, the generation of anti-idiotypic antibodies is well known in the art and can

O

readily be adapted to generate anti-idiotypic anti-84P2A9 antibodies that mimic an epitope on an 84P2A9 protein (see, for example, Wagner et al., 1997, Hybridoma 16: 33-40; Foon Set al., 1995, J. Clin. Invest. 96:334-342; Hcrlyn et al., 1996, Cancer Immunol. Immunother.

O 43:65-76). Such an anti-idiotypic antibody can be used in cancer vaccine strategies.

c1 10 Genetic immunization methods can be employed to generate prophylactic or

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Stherapeutic humoral and cellular immune responses directed against cancer cells expressing C 84P2A9. Constructs comprising DNA encoding an 84P2A9-related protein/ummunogen and appropriate regulatory sequences can be injected direcdy into muscle or skin of an individual, such that the cells of the muscle or skin take-up the construct and express the encoded 84P2A9 protein/immunogen. Alternatively, a vaccine comprises an 84P2A9related protein. Expression of the 84P2A9 protein immunogen results in the generation of prophylactic or therapeutic humoral and cellular immunity against bone, colon, pancreatic, testicular, cervical and ovarian cancers. Various prophylactic and therapeutic genetic immunuzation techniques known in the art can be used (for review, see information and references published at Internet address www.genweb.com).

KITS

For use in the diagnostic and therapeutic applications described herein, kits are also provided by the invention. Such kits can comprise a carrier being compartmentalized to receive in close confinement one or more containers such as vials, tubes, and the like, each of the container(s) comprising one of the separate elements to be used in the method. For example, the container(s) can comprise a probe that is or can be detectably labeled. Such probe can be an antibody or polynucleotide specific for an 84P2A9-realted protein or an 84P2A9 gene or message, respectively. Where the kit utilizes nucleic acid hybridization to detect the target nucleic acid, the kit can also have containers containing nucleotide(s) for amplification of the target nucleic acid sequence and/or a container comprising a reporter-means, such as a biotin-binding protein, such as avidin or Sstreptavidin, bound to a reporter molecule, such as an enzymatic, florescent, or 0 radioisotope label.

SThe kit of the invention will typically comprise the container described above and 5 one or more other containers comprising materials desirable from a commercial and user standpoint, including buffers, diluents, filters, needles, syringes, and package inserts with instructions for use. A label can be present on the container to indicate that the composition is used for a specific therapy or non-therapeuic application, and can also indicate directions for either in vivo or in vitro use, such as those described above.

S O p84P2A9-1 has been deposited under the requirements of the Budapest Treaty C'1 on January 6, 2000 with the American Type Culture Collection (ATCC), 10801 University Blvd., Manassas, VA 20110-2209 USA, and have been identified as ATCC Accession No. PTA-1151.

EXAMPLES

Various aspects of the invention are further described and illustrated by way of the several examples that follow, none of which are intended to limit the scope of the invention.

Example 1: SSH-Generated Isolation of cDNA Fragment of the 84P2A9 Gene Materials and Methods LAPC Xenografts and Human Tissues: LAPC xenografts were obtained from Dr. Charles Sawyers (UCLA) and generated as described (Klein et al, 1997, Nature Med. 3: 402-408). Androgen dependent and independent LAPC-4 xenografts LAPC-4 AD and AI, respectively) and LAPC-9 AD and AI xenografts were grown in male SCID mice and were passaged as small tissue chunks in recipient males. LAPC-4 and -9 AI xenografts were derived from LAPC-4 or

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N -9 AD tumors, respcctively. Male mice bearmg AD tumors were castrated and maintained for 2-3 months. After the tumors re-grew, the tumors were harvested and U passaged in castrated males or m female SCID mice. Human tissues for RNA and protein analyses were obtained from the Human Tissue Resource Center (HTRC) at the.

5 UCLA (Los Angeles, CA) and from QualTek, Inc. (Santa Barbara, CA). A benign prostatic hyperplasia tissue sample was patient-derived.

C"l Cell Lines: SHuman cell lines HcLa) were obtained from the ATCC and were IN 10 maintained in DMEM with 5% fetal calf serum.

RNA Isolation: Tumor tissue and cell lines were homogenized in Trizol reagent (Life Technologies, Gibco BRL) using 10 ml/ g tissue or 10 ml/ 108 cells to isolate total RNA.

Poly A RNA was purified from total RNA using Qiagen's Oligotex mRNA Mini and Midi kits. Total and mRNA were quantified by spectrophotometric analysis (O.D.

260/280 nm) and analyzed by gel electrophoresis.

Oligonucleotides: The following HPLC purified oligonucleotides were used.

DPNCDN (cDNA synthesis primer): 5'TTTGATCAAGC'TLo3' (SEQ ID NO: 7) Adaptor 1: 5'CTAATACGACTCACTATAGGGCTCGAGCGGCCGCCGGGCAG3' (SEQ ID NO: 8) (SEQ ID NO: 9) Adaptor 2: 5'GTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAG3' (SEQ ID IN 3'CGGCTCCTAG5' (SEQ ID NO: 11) O PCR primer 1: 5'CTAATACGACTCACTATAGGGC3' (SEQ ID NO: 12) Nested primer NP)1: O 5TCGAGCGGCCGCCCGGGCAGGA3' (SEQ ID NO: 13) Nested primer (NP)2: 5'AGCGTGGTCGCGGCCGAGGA3' (SEQ ID NO: 14) C 10 Suppression Subtractive Hybridization: SSuppression Subtractive Hybridization (SSH) was used to identify cDNAs corresponding to genes that may be differentially expressed in prostate cancer. The SSH reaction utilized cDNA from two LAPC-4 AD xcnografts. Specifically, the 84P2A9 SSH sequence was identified from a subtraction where cDNA derived from an LAPC-4 AD tumor, 3 days post-castration, was subtracted from cDNA derived from an LAPC-4 AD tumor grown in an intact male. The LAPC-4 AD xenograft tumor grown in an intact male was used as the source of the "tester" cDNA, while the cDNA from the LAPC-4 AD tumor, 3 days post-castration, was used as the source of the "driver" cDNA.

Double stranded cDNAs corresponding to tester and driver cDNAs were synthesized from 2 plg of poly(A)' RNA isolated from the relevant xenograft tissue, as described above, using CLONTECH's PCR-Select cDNA Subtraction Kit and 1 ng of oligonucleotide DPNCDN as primer. First- and second-strand synthesis were carried out as described in the Kit's user manual protocol (CLONTECH Protocol No. PT1117- 1, Catalog No. K1804-1). The resulting cDNA was digested with Dpn II for 3 hrs. at 370C. Digested cDNA was extracted with phenol/chloroform and ethanol precipitated.

Driver cDNA was generated by combining in a 1:1 ratio Dpn II digested cDNA from the relevant xenograft source (see above) with a mix of digested cDNAs derived from human benign prostatic hyperplasia (BPH), the human cell lines HeLa, 293, A431, Colo205, and mouse liver.

Tester cDNA was generated by diluting 1 pl of Dpn II digested cDNA from the

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Cl relevant xenograft source (see above) (400 ng) in 5 pl of water. The diluted cDNA (2 tl, 160 ng) was then hgated to 2 pl of Adaptor 1 and Adaptor 2 (10 NM), in separate ligation reactions, in a total volume of 10 pl at 16°C overnight, using 400 u of T4 DNA ligase l"- S 5 (CLONTECH). Ligation was terminated with 1 tl of 0.2 M EDTA and heating at 720C for 5 min.

The first hybridization was performed by adding 1.5 pl (600 ng) of driver cDNA to each of two tubes containing 1.5 pl (20 ng) Adaptor 1- and Adaptor 2- ligated tester C cDNA. In a final volume of 4 tl, the samples were overlaid with mineral oil, denatured

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S 10 in an MJ Research thermal cycler at 980C for 1.5 minutes, and then were allowed to

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CN hybridize for 8 hrs at 680C. The two hybridizations were then mixed together with an additional 1 tl of fresh denatured driver cDNA and were allowed to hybridize overnight at 68C. The second hybridization was then diluted in 200 pl of 20 mM Hepes, pH 8.3, mM NaC, 0.2 mM EDTA, heated at 70C for 7 min. and stored at PCR Amplification. Cloning and Sequencing of Gene Fragments Generated from SSH: To amplify gene fragments resulting from SSH reactions, two PCR amplifications were performed. In the primary PCR reaction 1 pl of the diluted final hybridization mix was added to 1 pl of PCR primer 1 (10 pM), 0.5 tl dNTP mix (10 tM), 2.5 pl 10 x reaction buffer (CLONTECH) and 0.5 pl 50 x Advantage cDNA polymerase Mix (CLONTECH) in a final volume of 25 pl. PCR 1 was conducted using the following conditions: 75°C for 5 min., 940C for 25 sec., then 27 cycles of 94oC for 10 sec, 660C for sec, 720C for 1.5 rmn. Five separate primary PCR reactions were performed for each experiment. The products were pooled and diluted 1:10 with water. For the secondary PCR reaction, 1 tl from the pooled and diluted primary PCR reaction was added to the same reaction mix as used for PCR 1, except that primers NP1 and NP2 (10 pM) were used instead of PCR primer 1. PCR 2 was performed using 10-12 cycles of 94CC for sec, 68°C for 30 sec, 720C for 1.5 minutes. The PCR products were analyzed using 2 agarose gel electrophoresis.

The PCR products were inserted into pCR2.1 using the T/A vector cloning kit S (Invitrogen). Transformed E. coli were subjected to blue/white and ampicillin selection.

O White colonies were picked and arrayed into 96 well plates and were grown in liquid culture overnight. To identify inserts, PCR amplification was performed on 1 ml of bacterial culture using the conditions of PCR1 and NP1 and NP2 as primers. PCR products were analyzed using 2% agarose gel electrophoresis.

Bacterial clones were stored in 20% glycerol in a 96 well format. Plasmid DNA was prepared, sequenced, and subjected to nucleic acid homology searches of the GenBank, dBest, and NCI-CGAP databases.

(N RT-PCR Expression Analysis: First strand cDNAs can be generated from 1 pig of mRNA with oligo (dT)12-18 priming using the Gibco-BRL Superscript Preamplification system. The manufacturer's protocol can be used and included an incubation for 50 min at 42oC with reverse transcriptase followed by RNAse H treatment at 370C for 20 mmn. After completing the reaction, the volume can be increased to 200 ptl with water prior to normalization. First strand cDNAs from 16 different normal human tissues can be obtained from Clontech.

Normalization of the first strand cDNAs from multiple tissues can be performed by using the primers 5'atatcgccgcgctcgtcgtcgacaa3' (SEQ ID NO: 15) and 5'agccacacgcagctcattgtagaagg 3' (SEQ ID NO: 16) to amplify p-actin. First strand cDNA (5 pl) can be amplified in a total volume of 50 pl containing 0.4 iM primers, 0.2 jIM each dNTPs, 1XPCR buffer (Clontech, 10 mM Tris-HCL, 1.5 mM MgCI2, 50 mM KCI, pH8.3) and IX Klentaq DNA polymerase (Clontech). Five pl of the PCR reaction can be removed at 18, 20, and 22 cycles and used for agarose gel electrophoresis. PCR can be performed using an MJ Research thermal cycler under the following conditions: Initial denaturation can be at 940C for 15 sec, followed by a 18, 20, and 22 cycles of 940C for 15, 650C for 2 min, 720C for 5 sec. A final extension at 72C can be carried out for 2 rmn. After agarose gel electrophoresis, the band intensites of the 283 bp P-actin bands from multiple tissues can be compared by visual inspection. Dilution factors for the first strand cDNAs can be calculated to result in equal P-actin band intensities in all tissues after 22 cycles of PCR. Three rounds of normalization can be required to achieve equal O band intensities in all tissues after 22 cycles of PCR.

To determine expression levels of the 84P2A9 gene, 5 ptl of normalized first Sstrand cDNA can be analyzed by PCR using 25, 30, and 35 cycles of amplification using primer pairs that can be designed with the assistance of (MIT; for details, see, www.genome.wi.mit.cdu).

Semi quantitative expression analysis can be achieved by comparing the PCR Sproducts at cycle numbers that give light band intensities.

O 10 Results O Two SSH experiments described in the Materials and Methods, supra, led to the isolation of numerous candidate gene fragment clones (SSH clones). Al candidate clones were sequenced and subjected to homology analysis against all sequences in the major public gene and EST databases m order to provide information on the identity of the corresponding gene and to help guide the decision to analyze a particular gene for differential expression. In general, gene fragments that had no homology to any known sequence in any of the searched databases, and thus considered to represent novel genes, as well as gene fragments showing homology to previously sequenced expressed sequence tags (ESTs), were subjected to differential expression analysis by RT-PCR and/or Northern analysis.

One of the SHH clones comprising about 425 bp, showed significant homology to several testis-derived ESTs but no homology to any known gene, and was designated 84P2A9.

Northern expression analysis of first strand cDNAs from 16 normal tissues showed a highly prostate and testis-related expression pattern in adult tissues (FIG. 4).

Example 2: Full Length Cloning of 84P2A9 A full length 84P2A9 cDNA clone (clone 1) of 2347 base pairs (bp) was cloned from an LAPC-4 AD cDNA library (Lambda ZAP Express, Stratagene) (Fig. The cDNA encodes an open reading frame (ORF) of 504 amino acids. Sequence analysis O revealed the presence of six potential nuclear localization signals and is predicted to be Snuclear using the PSORT program (http://psort.nibb.ac.jp:8800/form.html). The 0 protein sequence is homologous to a human brain protein KLAA1152 (39.5% identity Sover a 337 amino acid region), and exhibits a domain that is homologous to the 5 tumor suppressor protein (64.3% identity over a 42 amino acid region)(GenBank Accession #P52756)(Fig. The 84P2A9 cDNA was deposited on January 5, 2000 with the American Type Culture Collection (ATCC; Manassas, VA) as plasrrd p84P2A9-1, and has been assigned Accession No. PTA-1151.

u The 84P2A9 proteins have no homology to any known proteins, but the S 10 sequence does overlap with several ESTs derived from testis.

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Example 3: 84P2A9 Gene Expression Analysis 84P2A9 mRNA expression in normal human tissues was analyzed by Northern blotting of two multiple tissue blots (Clontech; Palo Alto, California), comprising a total of 16 different normal human tissues, using labeled 84P2A9 SSH fragment (Example 1) as a probe. RNA samples were quantitatively normalized with a p-actin probe. The results demonstrated expression of a 2.4 and 4.5 kb transcript in normal testis and prostate (Fig. 4).

To analyze 84P2A9 expression in prostate cancer tissues lines northern blotting was performed on RNA derived from the LAPC xenografts. The results show high levels of 84P2A9 expression in all the xenografts, with the highest levels detected in LAPC-9 AD, IAPC-9 AI (Fig. 4 and Figure These results provide evidence that 84P2A9 is up-regulated in prostate cancer.

In addition, high levels of expression were detected in brain (PFSK-1, T98G), bone (HOS, U2-OS), lung (CALU-1, NCI-H82, NCI-H146), and kidney (769-P, A498, CAKI-1, SW839) cancer cell lines (Fig. Moderate expression levels were detected in several pancreatic (PANC-1, BxPC-3, HPAC, CAPAN-1), colon (SK-CO-1, CACO-2, LOVO, COLO-205), bone (SK-ES-1, RD-ES), breast (MCF-7, MDA-MB-435s) and testicular cancer (NCCIT) cell lines (Fig.

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I In addition, prostate cancer patient samples show expression of 84P2A9 in both the normal and the tumor part of the prostate tissues (Fig. These results suggest that 0 84P2A9 is a very testis specific gene that is up-regulated in prostate cancer and Spotentially other cancers. Similar to the MAGE antigens, 84P2A9 may thus qualify as a cancer-tesus antigen (Van den Eynde and Boon, IntJ Clin Lab Res. 27:81-86, 1997).

84P2A9 expression in normal tissues can be further analyzed using a multi-tissue RNA dot blot containing different samples (representing mainly normal tissues as well as a few cancer cell lines).

S 10 Example 4: Generation of 84P2A9 Polyclonal Antibodies Polyclonal antibodies can be raised in a mammal, for example, by one or more injections of an immunizing agent and, if desired, an adjuvant. Typically, the immunizing agent and/or adjuvant will be injected in the mammal by multiple subcutaneous or intraperitoneal injections. Typically a peptide can be designed from a coding region of 84P2A9. Alternatively the immunizing agent may include all or portions of the 84P2A9 protein, or fusion proteins thereof. For example, the 84P2A9 amino acid sequence can be fused to any one of a variety of known fusion protein partners that are well known in the art such as maltose binding protein, LacZ, thioredoxmn or an immunoglobulin constant region (see, Current Protocols In Molecular Biology, Volume 2, Unit 16, Frederick M. Ausubul et al. eds., 1995; Linsley, Brady, Urnes, Grosmaire, L., Damle, and Ledbetter, L.(1991) J.Exp. Med. 174, 561-566). Other such recombinant bacterial proteins include glutathione-S-transferase (GST), and HIS tagged fusion proteins of 84P2A9 (which can be purified from induced bacteria using the appropriate affinity matrix).

It may be useful to conjugate the immunizing agent to a protein known to be immunogenic in the mammal being immunized. Examples of such immunogenic proteins include but are not limited to keyhole limpet hemocyanin, serum albumin, bovine thyroglobulin, and soybean trypsin inhibitor. Examples of adjuvants which may be employed include Freund's complete adjuvant and MPL-TDM adjuvant (monophosphoryl Lipid A, synthetic trehalose dicorynomycolate).

IN In a typical protocol, rabbits can be initially mumunized subcutaneously with i about 200 Cpg of fusion protein or KI-LH-peptide mixed in complete Freund's adjuvant.

Rabbits are then injected subcutaneously every two weeks with about 200 Pg of immunogen in incomplete Freund's adjuvant. Test bleeds are taken approximately 7-10 days following each immunization and used to monitor the titer of the antiserum by

ELISA.

Specificity of the antiserum is tested by Western blot and immunoprecipitation C analyses using lysates of genetically engineered cells or cells expressing endogenous S84P2A9. To genetically engineer cells to express 84P2A9, the full length 84P2A9 cDNA \O 10 can be cloned into an expression vector that provides a 6His tag at the carboxyl-tcrminus (pCDNA 3.1 myc-his, InVitrogen). After transfecuon of the constructs into 293T cells, cell lysates can be immunoprecipitated and Western blotted using anti-His or v5 antiepitope antibody (Invitrogen) and the anti-84P2A9 serum (see, FIG. 11). Sera from His-tagged protein and peptide immunized rabbits as well as depleted GST and MBP protein sera are purified by passage over an affinity column composed of the respective immunogen covalently coupled to Affigel matrix (BioRad).

Example 5: Production of Recombinant 84P2A9 in Bacterial and Mammalian Systems BACTERIAL CONSTRUCTS Production of Recombinant 84P2A9 using pGEX Constructs To express 84P2A9 in bacterial cells, a portion of 84P2A9 was fused to the Glutathione S-transferase (GST) gene by cloning into pGEX-6P-1 (Amersham Pharmacia Biotech, NJ). All constructs were made to generate recombinant 84P2A9 protein sequences with GST fused at the N-terminus and a six histidine epitope at the Cterminus. The six histiddine epitope tag was generated by adding the histidine codons to the cloning primer at the 3' end of the ORF. A PrcScission™l T recognition site permits cleavage of the GST tag from 84P2A9. The ampicillin resistance gene and pBR322 origin permits selection and maintenance of the plasmid in E. coli. In this construct, a fragment containing amino acids 1 to 151 of 84P2A9 was cloned into pGEX-6P-1.

Additional constructs can be made in pGEX-6P-I spanning regions of the 84P2A9 Sprotein such as amino acids 1 to 504 and amino acids 151 to 504.

MAMMALI.AN CONSTRUCTS To express recombinant 84P2A9 in mammalian systems, the full length 84P2A9 cDNA can for example, be cloned into an expression vector that provides a 6His tag at the carboxyl-terminus (pCDNA 3.1 myc-his, InVitrogen). The constructs can be t transfected into 293T1 cells. Transfected 293T cell lysates can be probed with the anti- 01 84P2A9 polyclonal serum described in Example 4 above in a Western blot.

10 The 84P2A9 genes can also be subcloned into the retroviral expression vector

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O pSRaMSVtkneo and used to establish 84P2A9 expressing cell lines as follows. The Cl 84P2A9 coding sequence (from translation initiation ATG to the termination codons) is amplified by PCR using ds cDNA template from 84P2A9 cDNA. The PCR product is subcloned into pSRaMSVtkneo via the EcoRl(blunt-ended) and Xba 1 restricton sites on the vector and transformed into DH5x competent cells. Colonies are picked to screen for clones with unique internal restriction sites on the cDNA. The positive clone is confirmed by sequencing of the cDNA insert. Retroviruses may thereafter be used for infection and generation of various cell lines using, for example, NIH 3T3, TsuPrl, 293 or rat-1 cells.

Specific mammalian systems are discussed herein.

Production of Recombinant 84P2A9 using pcDNA3.1/V5-His-TOPO Constructs To express 84P2A9 in mammalian cells, the 1512 bp (504 amino acid) 84P2A9 ORF along with perfect translational start Kozak consensus sequence was cloned into pcDNA3.1/V5-His-TOPO (Invitrogcn, Carlsbad, CA). Protein expression is driven from the cytomegalovirus (CMV) promoter. The recombinant protein has the epitope and six histidines fused to the C-terminus. The pcDNA3.1/V5-His-TOPO vector also contains the bovine growth hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability along with the origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen. The Neomycin resistance gene allows for selection of mammalian cells expressing the protein and the ampicillin resistance gene and ColE1 origin permits selection and maintenance of the plasmid in E. coli.

eU SpSRa Constructs To generate mammalian cell lines expressing 84P2A9 constitutively, the 1551 bp (517 amino acid) ORF is being cloned into pSRa constructs. Amphotropic and ecotropic retroviruses are generated by transfection of pSRa constructs into the 293T-10A1 packaging line or co-transfection of pSRa and a helper plasmid in 293 cells, respectively. The retrovius can be used to infect a variety of mammalian cell lines, S 10 resulting in the integration of the cloned gene, 84P2A9, into the host cell-lines. Protein Sexpression is driven from a long terminal repeat (LTR). The Neomycin resistance gene 1 allows for selection of mammalian cells expressing the protein and the ampicillin resistance gene and ColEI origin permits selection and maintenance of the plasmid in E.

coli. Additional pSRa constructs are being made to produce both N-terminal and Cterminal GFP and myc/6 HIS fusion proteins of the full-length 84P2A9 protein.

Example 6: Production of Recombinant 84P2A9 in a Baculovirus System To generate a recombinant 84P2A9 protein in a baculovirus expression system, the 84P2A9 cDNA is cloned into the baculovirus transfer vector pBlueBac (Invitrogen), which provides a His-tag at the N-terminus Specifically, pBlueBac-- 84P2A9 is co-transfected with helper plasmid pBac-N-Blue (Invitrogen) into SF9 (Spodoptera frugiperda) insect cells to generate recombinant baculovirus (see Invitrogen instruction manual for details). Baculovirus is then collected from cell supernatant and punfied by plaque assay.

Recombinant 84P2A9 protein is then generated by infection of HighFive insect cells (InVitrogen) with the purified baculovirus. Recombinant 84P2A9 protein may be detected using anti-84P2A9 antibody. 84P2A9 protein may be purified and used in various cell based assays or as immunogen to generate polyclonal and monoclonal antibodies specific for 84P2A9.

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Example 7: Chromosomal Mapping of the 84P2A9 Gene C1 The chromosomal localization of 84P2A9 was determined using the GencBridge4 radiation hybrid panel (Walter et al., 1994, Nat. Genetics 7:22) (Research Genetics, Huntsville Al). The following PCR primers were used to localize 84P2A9: 84P2A9.1 gacttcactgatgcgatggtaggt (SEQ ID NO: 17) 84P2A9.2 gtcaatactttccgatgctttgct (SEQ ID NO: 18) The resulting mapping vector for the 93 radiation hybrid panel DNAs was: 0000100011001011001000001100010010010000100010100110001000000010010010010 10000000100000010000. This vector and the mapping program at http://wwwgenome.wi.mit.edu/cgi-bin/contig/rhmapper.pl placed 84P2A9 on chromosome 1lq 3 2 3 (DI1S1 602- D1S217).

Example 8: Identification of Potential Signal Transduction Pathways To determine whether 84P2A9 directly or indirectly activates known signal transduction pathways in cells, luciferase (luc) based transcriptional reporter assays are carried out in cells expressing 84P2A9. These transcriptional reporters contain consensus binding sites for known transcription factors that lie downstream of well characterized signal transduction pathways. The reporters and examples of there associated transcription factors, signal transduction pathways, and activation stimuli are listed below.

1. NFkB-luc, NFkB/Rel; Ik-kinase/SAPK; growth/apoptosis/stress 2. SRE-luc, SRF/TCF/ELK1; MAPIK/SAPK; growth/differenntiation 3. AP-1-luc, FOS/JUN; MAPK/SAPK/PKC; growth/apoptosis/stress 4. ARE-luc, androgen receptor; steroids/MAPK; growth/differentiation/apoptosis p53-luc, p 5 3 SAPK; growth/differentiation/apoptosis 6. CRE-luc, CREB/ATF2; PKA/p38; growth/apoptosis/stress I 84P2A9-mediated effects may be assayed in cells showing mRNA expression.

SLuciferase reporter plasmids may be introduced by lpid mediated transfecton U Promega). Luciferase activity, an indicator of relative transcrptional activity, is measured Sby incubauon of cells extracts with luciferin substrate and luminescence of the reaction is monitored in a luminometer.

Example 9: Generation of 84P2A9 Monoclonal Antibodies To generate MAbs to 84P2A9, typically Balb C mice are immunized intraperitoneally with about 10-50 p.g of protein immunogen mixed in complete Freund's S 10 adjuvant. Protein immunogens include bacterial and baculovirus produced recombinant "i 84P2A9 proteins and mammalian expressed human IgG FC fusion proteins. Mice are then subsequently immunized every 2-4 weeks with 10-50 plg of antigen mixed in Frcund's incomplete adjuvant. Alternauvely, Ribi adjuvant is used for initial immunizations. In addition, a DNA-based immunization protocol is used in which a mammalian expression vector such as pCDNA 3.1 encoding the 84P2A9 cDNA alone or as an IgG FC fusion is used to immunize mice by direct injection of the plasmid DNA.

This protocol is used alone and in combination with protein immunogens. Test bleeds are taken 7-10 following immunization to monitor titcr and specificity of the immune response. Once appropriate reactivity and specificity is obtained as determined by ELISA, Western blotting, and immunoprecipitation analyses, fusion and hybridoma generation is then carried with established procedures well known in the art (Harlow and Lane, 1988).

In a typical specific protocol, a glutathione-S-transferase (GST) fusion protein encompassing an 84P2A9 protein is synthesized and used as immunogen. Balb C mice are initially immunized intraperitoneally with 10-50 tg of the GST-84P2A9 fusion protein mixed in complete Freund's adjuvant. Mice are subsequently immunized every 2 weeks with 10-50 pg of GST-84P2A9 protein mixed in Freund's incomplete adjuvant for a total of 3 immunizations. Reactivity of serum from immunized mice to full length 84P2A9 protein is monitored by ELISA using a partially purified preparation of HIStagged 84P2A9 protein expressed from 293T cells (Example Mice showing the

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0 strongest reactivity are rested for 3 weeks and given a final injection of fusion protein in C, PBS and then sacrificed 4 days later. The splcens of the sacrificed mice are then harvested and fused to SPO/2 myeloma cells using standard procedures (Harlow and Q Lane, 1988). Supernatants from growth wells following HAT selection are screened by ELISA and Western blot to identify 84P2A9 specific antibody producing clones.

The binding affinity of an 84P2A9 monoclonal antibody may be determined using standard technology. Affinity measurements quantify the strength of antibody to epitope binding and may be used to help define which 84P2A9 monoclonal antbodies are preferred for diagnostic or therapeutic use. The BIAcore system (Uppsala, Sweden) O 10 is a preferred method for determining binding affinity. The BlAcore system uses surface Splasmon resonance (SPR, Welford K. 1991, Opt. Quant. Elect. 23:1; Morton and '1 Myszka, 1998, Methods in Enzymology 295: 268) to monitor biomolecular interactions in real time. BlAcore analysis conveniently generates association rate constants, dissociation rate constants, equilibrium dissociation constants, and affinity constants.

Example 10: In Vitro Assays of 84P2A9 Function The expression of 84P2A9 in prostate cancer provides evidence that this gene has a functional role in tumor progression. It is possible that 84P2A9 functions as a transcription factor involved in activating genes involved in tumorigenesis or repressing genes that block tumorigenesis. 84P2A9 function can be assessed in mammalian cells using in vitro approaches. For mammalian expression, 84P2A9 can be cloned into a number of appropriate vectors, including pcDNA 3.1 myc-His-tag (Example 5) and the retroviral vector pSRatkneo (Muller ct al., 1991, MCB 11:1785). Using such expression vectors, 84P2A9 can be expressed in several cell lines, including NIH 3T3, rat-1, TsuPrl and 293T. Expression of 84P2A9 can be monitored using anti-84P2A9 antibodies (see Examples 4 and 9).

Mammalian cell lines expressing 84P2A9 can be tested in several in vitro and in vivo assays, including cell proliferation in tissue culture, activation of apoptotic signals, tumor formation in SCID mice, and in vitro invasion using a membrane invasion culture system (MICS) (Welch et al. ,Int. J. Cancer 43: 449-457). 84P2A9 cell phenotype is

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Scompared to the phenotype of cells that lack expression of 84P2A9. The transcriptional effect of 84P2A9 can be tested by evaluating the effect of 84P2A9 on gene expression using gene arrays (Clontech) and transcriptional reporter assays (Stratagene).

Q Cell lines expressing 84P2A9 can also be assayed for alteration of invasive and migratory properties by measuring passage of cells through a matrigel coated porous membrane chamber (Becton Dickinson). Passage of cells through the membrane to the opposite side is monitored using a fluorescent assay (Becton Dickinson Technical 1 Bullein #428) using calcein-Am (Molecular Probes) loaded indicator cells. Cell lines analyzed include parental and 84P2A9 overexpressing PC3, NIH 3T3 and LNCaP cells.

10 To determine whether 84P2A9-expressing cells have chemoattractant properties, Sindicator cells are monitored for passage through the porous membrane toward a

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gradient of 84P2A9 conditioned media compared to control media. This assay may also be used to qualify and quantify specific neutralization of the 84P2A9 induced effect by candidate cancer therapeutic compositions.

The function of 84P2A9 can be evaluated using anti-sense RNA technology coupled to the various functional assays described above, e.g. growth, invasion and migration. Anti-sense RNA olgonucleotides can be introduced into 84P2A9 expressing cells, thereby preventing the expression of 84P2A9. Control and anti-sense containing cells can be analyzed for proliferation, invasion, migration, apoptotic and transcriptional potential. The local as well as systemic effect of the loss of 84P2A9 expression can be evaluated.

Example 11: In Vivo Assay for 84P2A9 Tumor Growth Promotion The effect of the 84P2A9 protein on tumor cell growth may be evaluated in vivo by gene overexpression in tumor-bearing mice. For example, SCID mice can be injected SQ on each flank with 1 x 10 6 of either PC3, TSUPR1, or DU145 cells containing tkNeo empty vector or 84P2A9. At least two strategies may be used: Constitutive 84P2A9 expression under regulation of a promoter such as a constitutive promoter obtained from the genomes of viruses such as polyoma virus, fowlpox virus (UK 2,211,504 published July 1989), adenovirus (such as Adenovirus bovine papilloma virus, avian sarcoma

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virus, cytomegalovirus, a retrovirus, hepatis-B virus and Simian Virus 40 (SV40), or from heterologous mammalian promoters, the actin promoter or an immunoglobulin 0 promoter, provided such promoters are compatible with the host cell systems, and (2) SRegulated expression under control of an inducible vector system, such as ecdysone, tet, etc., provided such promoters are compatible with the host cell systems. Tumor volume is then monitored at the appearance of palpable tumors and followed over time to determine if 84P2A9 expressing cells grow at a faster rate and whether tumors produced by 84P2A9-expressing cells demonstrate characteristics of altered aggressiveness (e.g.

enhanced metastasis, vascularization, reduced responsiveness to chemotherapcutic C 10 drugs). Additionally, mice may be implanted with 1 x 10 5 of the same cells orthotopically 0 to determine if 84P2A9 has an effect on local growth in the prostate or on the ability of C the cells to metastasize, specifically to lungs, lymph nodes, and bone marrow.

The assay is also useful to determine the 84P2A9 inhibitory effect of candidate therapeutic compositions, such as for example, 84P2A9 intrabodies, 84P2A9 antisense molecules and ribozymes.

Example 12: Western Analysis of 84P2A9 Expression in Subcellular Fractions Sequence analysis of 84P2A9 revealed the presence of nuclear localization signal.

The cellular location of 84P2A9 can be assessed using subcellular fractionation techniques widely used in cellular biology (Storrie B, et al. Methods Enzymol.

1990;182:203-25). Prostate or testis cell lines can be separated into nuclear, cytosolic and membrane fractions. The expression of 84P2A9 in the different fractions can be tested using Western blotting techniques.

Alternatively, to determine the subcellular localization of 84P2A9, 293T cells can be transfected with an expression vector encoding HIS-tagged 84P2A9 (PCDNA 3.1 MYC/HIS, Invitrogen). The transfected cells can be harvested and subjected to a differential subcellular fractionation protocol as previously described (Pemberton, P.A. et al, 1997, J of Histochemistry and Cytochemistry, 45:1697-1706.) This protocol separates the cell into fractions enriched for nuclei, heavy membranes (lysosomes, peroxisomes, and mitochondria), light membranes (plasma membrane and endoplasmic reticulum), and soluble proteins.

5 Throughout this application, various publications are referenced (within parentheses for example). The disclosures of these publications are hereby incorporated by reference herein in their entireties.

The present invention is not to be limited in scope by the embodiments disclosed N 10 herein, which are intended as single illustrations of individual aspects of the invention,

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Sand any that are functionally equivalent are within the scope of the invention. Various Cl modifications to the models and methods of the invention, in addition to those described herein, will become apparent to those skilled in the art from the foregoing description and teachings, and are similarly intended to fall within the scope of the invention. Such modifications or other embodiments can be practiced without departing from the true scope and spirit of the invention.

TABLES

TABLE 1: predicted binding of peptides from 84P2A9 proteins to the human MHC class 1 molecule HLA-A2 Rank Start Subsequence Residue Listing Score (Estimate of half Position time of disassociation) 1 300 SILTGSFPL (SEQ ID NO: 19) 63.04 2 449 RMLQNMGWT (SEQ ID NO: 20) 33.75 3 4 LVHDLVSAL (SEQ ID NO: 21) 29.97 4 238 SLSSTDAGL (SEQ ID NO: 22) 21.36 198 KIQDEGVVL (SEQ ID NO: 23) 17.28 6 433 FVGENAQPI (SEQ ID NO: 24) 17.22 7 301 ILTGSFPLM (SEQ ID NO: 25) 16.05 8 218 KMECEEQKV (SEQ ID NO: 26) 11.25 9 480 KGLGLGFPL (SEQ ID NO: 27) 10.47 461 GLGRDGKGI (SEQ ID NO: 28) 10.43 TABLES 3-16 provide additional analyses of the predicted binding of peptides from 84P2A9 proteins to various HLA molecules.

2006249244 07 Dec 2006 TABLE 2: AMINO ACID SUBSTITUTION MATRIX Adapted from the GCG Software 9.0 BLOSUM62 am-ino acid substitution matrix (block substitution matrix). TVhe higher the value, the more likely a substitution is found in related, natural proteins.

A C D E F G 1-1 1 K L M N P Q R S -r v w Y 4 0 -2 -1 -2 0 -2 -1 -1 -1 -1 -2 -1 -1 -1 1 0 0 -3 -2 A 9 -3 -4 -2 -3 -3 -1 -3 -1 -1 -3 -3 -3 -3 -1 -1 -1 -2 -2 C 6 2 -3 -1 -1 -3 -1 -4 -3 1 -1 0 -2 0 -1 -3 -4 -3 D -3 -2 0 -3 1 -3 -2 0 -1 2 0 0 -1 -2 -3 -2 E 6 -3 -1 0 -3 0 0 -3 -4 -3 -3 -2 -2 -1 1 3 F 6 -2 -4 -2 -4 -3 0 -2 -2 -2 0 -2 -3 -2 -3 G 8 -3 -1 -3 -2 1 -2 0 0 -1 -2 -3 -2 2 H 4 -3 2 1 -3 -3 -3 -3 -2 -1 3 -3 -1 1 -2 -1 0 -1 1 2 0 -1 -2 -3 -2 Ic 4 2 -3 -3 -2 -2 -2 -1 1 -2 -1 L -2 -2 0 -1 -1 -1 1 -1 -1 MI 6 -2 0 0 1 0 -3 -4 -2 N 7 -1 -2 -1 -1 -2 -4 -3 1) 1 0 -1 -2 -2 -1 Q -1 -1 -3 -3 -2 R 4 1 -2 -3 -2 S 0 -2 -2 T 4 -3 -1 N7 11 2 \X/ 7 Y lILA peptide motif search results User Paraweters and Scoring Information i Ma I-idto limint nc of results repii uber nuxnier of results requested 3 M HA molecule type selected 17 length selcat or subsequences to be scored-- ~echoing mode selected for input equence- echoing format- nubred lines *_length of user's* input peptide sequence ;___504 number of subsequence scores calculated 496 ;Inumbcr of top-sconng subsequences reported back in scorig output table.1 TABLE 3A Scoring Results RankStar ~ace Residue ;IScore (Estimate of.Half Ti of Disassociation o o~itioU ~Listinga Molecule Containing This Susfu]ce SLEEFSKOY 445.000 SEPIQAMQ 27.000 F~I 283KEDFTELDK 25.00 4 15SSEARGG-F 13.500 900 F6-- 441 7F LENNIGNR 9.000 241 j TDgAGLFrN I6.250 _ESDSSSLSS I- 3.750 lof DSOOQMLVA' 3.750 j i MTQPREGCR 2.500 i2j 13 TGDIK~tRRK F 2 2500 1- F 1 2.250 ______RTEHOOH L 2.2 6 ES S L1.350 F' 1.250 2417 238 IF SETNSSSL 196- 79 0L .9500 731Gi71S P HDHLR 0 .750 Echoed User Peptide Sequence (Iength =504 residues) !XfELVHDLVS S YN VHH PWET

KRRPSSNLNNJ

!SNKRTMTQP

DEGVVLESEE

EGR0GDDE0S

ILTGSFPLMS

NKM~VKFSPD

SRQTSMHLGS

LQNMGWTPGS

GKSA

ALEESSEQAR

GHCLSEGS

NVRGKRPLWH

PEG3CRDQDMD

TNQTNKDKME

DWFYEKESGG

HPSRRGFQAR

SHH H DRWFS P

LCTGDIKRRR

GLGRDGKGIS

GG FAETGOXS

SLEEPSKDYR

ESOFAVDNVG

SDRAYQYQEF

C EEQK VS DEL

ACGTTGVVPW

LSRLHGMSSK

GARTEHDQHQ

KAA PL.PCPTT

EPIQAM~QRPK

RSISCPLKRQ

ENHNNNKKDH

NRTLRRRKV

TKNKVKKRIKL

MSESDSSSLS

WEKEDPTELO

NIKKSGGTPT

LLRDNRAERG

AGFVGENAQP

GLGLGFFLPK

ARFCRRGRKRR

ST)SDDQMLVA

KMAVDL PQD

KIIRQGPKIQ

7T DAG L FTN D 1\NVPDPV FES SMVPI PGPVG

HKKNCSVRTA

ILENNIGWP.M

STSATTTPNA

TABLE 3B HLA peptide motif search results us -aaetr an Scoring Information mcffhod s-elete-d to hit umbr of results HExplcznllibcr number of results requested KHLA molecule type selected A__ -length selected for subsequences to be scored I........echoing mode selected for input se-quence -length of user's input peptide sequence_ :j 504___ number of subsequence scores calcul-ated- f 495____ Inumberoftop-sconng subsequences reported back in scoring output ta~Fe:L[-- 3 TABLE 4A ______Scoring esu-Ib Listi:oeue otiigTisSbeune Rak Start Position! SbquneR ide Score (Estimate of Half Time of Disass0cao of T ISrpiQAMQR F 7 921 DSD Dq MLVAK f30.000 ESEtNQTNK 27.000 r74-i1 6 nDVSdipAYoY 2500 j~3T[ iij ALEEssiQAR .900 I f 71 S;LEEpSKDYR {9.00 F~7[2827 EpETLDK 4e506- fl~7 ~DQDMCISDRAY f3.756 1 HDQDMLVj 3.750 T0j 177 YQEFtKVK .1 2.700Or f.iI. 14.4 AV pQDISN ]-2.500 RTEMdQHQLL j2.250 7i37T3 __*1SSL 1.350 214 C~ODF 1.250 ~GDDEq5DWFY FIT- _VPD vFESIL 1.250 9~7 173 AYQyQETK [1.000 _GLGLgFPLPK [1.000 21 r2 i VLESeETNQT [0.900 F F7 YRE',hN',NKK 0.900 24f 23 FAE;TgtDHSRS 0.900 121 ES;DFaVDV .775 r2 233075___ J27! 4091 GSLCtGDIKR [7 ~~SLePTD ~9 f 0.750 JV6 rsos3LEEPS 0.750 Echoed User Peptide Sequence (length =504 residues) MEELVH DLVS

SYNVHHPWET

KRRPSSNLNN

I SNKRiTMTOP

DEGVVLESEE

EGRQGDDEQS

ILTC.SFPLMS

NKR.M1VHFSPO

SROTSMHLGS

LQNIIGWTPGS

GKSA

ALE ESS EQAR

GHCLSEGSOS

NVRGKRPLWH

PEGCRDQDMD

TNQTNKDKME

DWFY7KESGG H P SRAGFQPLR S HHH O W FS P

LCTGDIKRRR

GLGRDGKG IS

GGFAZTGOMS

SLEEPSKOYR

ESDFAVDNVG

SDRAYQYQCF

CEEQKVS DEL ACC ITGVVPW

LSRLHGMSSK

GARTEHDOHQ

KAAPLPGPTT

EPIQAZIQRPK

RSISCPLKRQ

ENHNNNKKDH

NRTLRRRP.Kv

TKNXVKKRKL

MSESDSSSLS

WE!(EDPTELD

NI!<KSGGtPT

LLRDWRAERG

AGFVGENAQP

GLGLGFPLPK

ARKRRCRKRR

SOSDD(QMLVA

KRHAVDLPQO

KILRQGPKIQ

STDAGL FTND

KNVPDPVFES

SMVPI PGPVG

HKKNCSVRTA

ILENNICNRM4

STSATTTPNA

TABLE 4B

IN

HILA peptide motif search results F _User Parameters and Scoring Information7 method sel ectedf to lut number ot results [explicit numberi number of results requested 3 H.LA molecule type selected A0201F~ length selecte for subsequences to be scored 9 echoing modec selected for input sequence echoingformat_71 numbre linaes Slength of user's input peptbdEe Snumber of subsequence score ca culated 496 :Inumber of top-scoring subsequences reported backIn scoring output table- 1 TABLE SA I ~SCOrin-gResults Rank-, Start Position Susq e ,sidue :jScore (Estimate of HalIT Time of Disassociation of* Listing I a Molecule Containing This Subsequence) SILTGSFPL 6.3 L~.7447Z RMLQNMGW r 32.74 4 N7~7Z. LMLSAL 29~- .965 238 SL~LSSTDAGL '121.362 KIOEGVVL 11.:9 22 433. .FGENAQPI -17.217-. SILTGSFP 16.047 t47~TL KGLGLGFPL I fW 1 46~ f :[__SMVPIPGPV 6 .330 12 468 6.442___ FT.1f 405 SMHLSLC L[ 312____ F1~[ 11 r KIIP.QGPKI 5.0211 FT71-* 17 PLWHESDFA .[2.4451 177 '1 YQEFTKNKV 2.076 17 7 3 4 T f MGWTPGSGL 1.968 1 1 56 TMTQPPEGC ____1.758 1- 3.74 TEHDQHOQLL 1.703 [=0L .A~YNVHHPWE T 1. 1.6 78- SQAMQRPKG 1.9 F1271 240TOGLFT 1.097 1231 38 1 AQE'ILENNI *1.8 V?4 1.269j GGACGITGV .044 F 257I4 MAVDLPQDI EMU-01 Echoed User Peptide Sequence (length 504 residues)

MEELVHDLVS

SYNVH41PWET

KRRPSSNLNN

I SNKRTMTQP

DEGVVLESEE

EGRQCDDZQS

ILTGSF?LMS

NKP~RMVHFSPD

SRQTSMHLGS

LQNMGWTPGS

GKSA

ALEESSEQAR

GHCLSEGSDS

NV RG KR PLWH

PEGCRLDQCMD

TNQTNKDKME

DWFYEKESGG

RPSRr<GFQAR S HHHOH WFS P

LCTCDIKPCRR

GLGRDGKGIS

GG FAETGDHS

SI.EEPSKOYR

ES DFAVDNVG

SDRAYQYQEF

CEEOKVS DEL

ACCITGVVPW

LSRLHGMSSK

GARTER DQHQ

KAAPLPGPTT

EPIOAMORPK

RSISCPLKRQ

ENHNNNKKDH

NRTLRRRRXV

TK<NKVKKRKL

MSESDSSSLS

WEKEDPTELD

NIKKSGGTPT

LLRI2NRAERG

AGFVGENAQP

GLGLGFPLPK

ARKRRGRKRR

SOSDOOMLVA

KRMAVDLPQD

KIIRQGPKIQ

ST DAGL FT ND

KNVPOPVFES

SMVPIPGPVC

HKKNCSVRCA

I LENNIC.NRM

STSATTTPNA

TABLE LILA peptide motif search results length selected for SubsequenCeS t esoe econ-oeslce for input sequence echoing format 0 egho u ses Iiputl F,p~epdesequence I number of subsequence scorscluad ofto-scoig subsequences rejported back in scoring output tat)e TABLE 6A Ran Sartp 0 1 ti~;Susequence Residue Scor Hal ime of f a Molecule Containing ThisSubsequence) 230 f LMSEsD 07L L77___63 -CLSEgSDSSL *Li~L....mi.ees FAV 73.66 1 47 1542 -F 6 1 433 I FVCEnAQPIL 4 T F 31RLHGnS SKNI 1.3 1 42 F~Ti[7_3~ RMAVdL PODI 7.535 GLGFpLPKST 7.4521 SILTgSFPLM 4.8021 3.685 IQA~~qRPK(CL [362 NVP .DpV FES I 3.4851 FAVDnVGjR KSGCtP~T 1.589 NIGNrM1LQNN 1.5711 RGFOaRLSL1.187 SGGAcGITGV--.[ 1.04" ELDKnVPDPV [1.022 FPLPkSTSAT {0.828 VLESeETNOT 0.811 RQTSrnHLC.SI,.. .0.648 LPGPtTA GFV0.2 ILENnIGN1 0. 541I !SSLS 3TDAGL 0.516 .2L6 -1SALEeS9EOA .0.513 26 10 L .04_ -f-77-11 212 7QT Ck0.5EC4 30 1 IZTGSFPLMS 0.481 I LSSTCtAGLFET _0.455 [0 iU RPSSriLNNNV {0.454 Echoed User Peptide Sequence (length 504 residues)

MEELVHDLVS

SYNVHiHPWET

KRRPSSNLWN

ISNKRTMTQP

DEGVVLESEE

EGRQGDDEQS

ILTGSFPLMS

NKRNVHFSPD

SRQTS,%iHLGS LQNMGWT PGS

GK(SA

ALEESSEQAR GGFAETGDI{S GHCLSEGSDS SLEEPSKDYR NVRGKnPLWH ESDFAVDNVG PEGCRDQDMD SORAYQYQEF TNQTNKDKME CETQKVS DEL OWFYEMESGG ACGITGVVPW HPSRRGFQAR LSRLHMGMSSK SHHHDHWFSP GARTEHDQHQ LCTGDIKRRR KAAPLPCPTT GLGRDGKGIS EPIQAMQRPK

RSISCPLKRQ

ENHNN4NKKDfl

NRTLELRRRKV

TKNKVKKRKL

MSESDSSSLS

WEKEDPTELD

NIKKSGGTPT

LLRONRAERG

AG FVGENAQ P

GLGLGFPLPK

ARKRRGRKRR

SOS DDQML VA KRMAV DL POD

KIIRQGFKIQ

STDAGLFTND

KNVPDPVCES

SMVPI PGVVG

HNKNCSVRTA

ILENNIGNRM

STSATTTPNA

TABLE 6B HLA peptide motif search results User Parameters and Scoring Information method selected to limit ntumber of results f- ~ii nurnber.

number o results requested a.1A molecule type selected A3 length selected for subsequences to be scored_ ehon moeslce for input sequence .Iechoing fornmt numbe lines length of useT~s input pepTide -sequence n-umber of subsequence scores calculated Inuniber of top-scoring subsequences repod back in scaring output table] 1 TABLE 7A Scoring Results INDSubsequence ReCsidaue* Score (Estiate of HTme o islss ciation o~ RankStal Psitons Listing 2~ Molecule Contaiing This Subsequct_ S36GMSSKNIKK2000 -3 3TLRARRKVK ILL 7F 14-I46 DLPODISNK 90 0 410 SLCTCIDIKR F 6- NLN%4NVRGK600 F 58 7EQSDWFYEK 480-- F 71 SL-EEPSDY 4.50-- 9 381 i LLRONRAER Zi .00 FFFI 41 f ILENIGNR 1 :.800.- T ATTTPNG 1.500 301 !LTG-SFPLM 0.0 0 .1 0 0.900F -7 1 2 8 NVGNRTR FT7TAC 0.1606 6WTPGSGLGP.0.0 3. 19 KE PTEL K ORO4 7 GLCEIK 42~ 23~ 0.405 18 L.KMKLKI W 00_ N156 TMTOPEGC F 251 11 :1 A1.ZESSEQA0.0 26 .1 180 L 72T 35 -CFLKFRK I-030 rrG~Z L SGLGRDGK 0.36(0 T9 I R L KIIRQGP K 0.20 K I I R P P K Echoed User Peptide Sequence (length =504 residues) I MEELVHDLVS ALESSEQAR GGFA]&TGDHS RSISCPLKRO

ARKRRGRKRR

51 SYNVHWET GHCLSEGSOS SLEEPSKDYR ENHNNNKKUH

SOSODMLVA

101 IRSSNLNN NVRGKRPLWH ESDFAVDNVG NRTLRRRRKV KRMAVDLP?0D 151 ISNKRTMTQP PECCRDODMV) SEORAYQYQEF TKNKVKKRKL KiIRQGPKIQ 201 DZ-GVVLESEE TNQTNKD1Q1E CEEQKVSDEL MSZSDSSSLS

STDAGLFTNO

251 EGRQGDDEQS [DWFYE-KZSGG ACGITGVVPW WEKEDPTELD

KNVPDPVFES

O1 LTFPS 4$R'QRLSRLHGMSSK NIKKSGGTPT SmvPIPGPVG 351 N:KRMVRFSPD SHHHDHWiVSP GARTEHOQHQ LLRDNRAERG flKKCSVRTA 401 SROTSXHLGS LCTGDIKP.RR KAAPLPGPTT AGFVGENAQP

ILENNIGNRM

451 LQNMGWTPGS GLGRDCKGIS EPIOAMrQRPK GLGLGFPLPK

STSATTTPNA

501 GKSA TABLE7B 96 HLA peptide motif search resultis User Parameters and Scoring Information method selected to limit nun-er ot results xl!t-=zbri number of results requesie&- 30-- ume LILA molecule type selected A I ~length selected for subsequences to be scored 0 echoing mode selected for input sequence [i 7y echoing format nubee___ slength of user's input peptide sequence number of subsequence scores calculated InurnbL7 of top- sconng subsequences reported back in scoring output tableE TABLE 8A .Scoring Resu ts tr oitoi6bs itng s Score (Estimate of Hal RankiStar Po istig f a Molecle Containng GLGLgFPLPK36.

7T 7i7 SLE':EpSK)YR ALEXsSEQAR IF 6.00 Q LLR dNRAER1 6 f 175. YQYQeFTKNK I4.50 27I[TGVvPWWEK -4.50 P77K1 13 T-lRIrKVK~___ I 168 DMDSdRAYQY fl~; 173 RYyETK -3.00 FTF7 411 SLCTgDIKRR

F

K!I 1 6 t TMTQpPEGCR 1T~I 16~[ DPQdISiKR 1.8D' 11L. N"NNnVRGKR .I.0 [-T7..O!RpKGLGL M 63- CLS____gSO 0.90 NMGWtPGSGL0.i 38 3-0 LM.SEsDS SSLI09 .F9 3 LVHdLV 0.81 S180FTKNkVCR KF771[ 34 VPI?gPVGNK 0.601 1r72W11

SSCDAGLF

142 RrAVdLPQOI 0.60( F73 r_257~ DEQSdWFE 0.481 1 2 323 L HGUS S KN T I -0.45 S7- 31~ ILTGsFPLMS .[0.36 7E11 PLWHeSDFAV 2V [493 If SATTtPNAGK YQE~r.KNKVK Echoed User Peptide Sequence (length =504 residues)

MEELVHDLVS

S YN VHH PWET

KRRPSSNLNN

I SNKRTMTQP

DEGVVLESEE

EGRQGDDEQS

ILTG'3FPU S NKRM'VH FSPD

SRQTSMHLGS

LQNMGWT PGS

GKSA

TABLE 8B3 ALE ES SEQAR

GHCLSEGSDS

NVRGKRPLWH

PEGCRDQDMD

TNOTNKDKM'E

DWFYEKESGG

HPSRRGFQAR

SHHHDHWFSP

LCTGDIgKRR GLGRDGKGis GGrIAETGDHS S LEE PS KOYR ES DFAVONVG

SORAYQYQEE'

CEEQKVS DEL ACG ITGVVPW

LSRLHGHSSK

GARTEHDQHQ

KAAPLPGPTT

EPIQAMQRPK

RS ISCPLKRQ

ENHNNNKKDH

NRTLRRRRKV

TKNKVKKRKL

MSESDSSSLS

WEKEDPTELD

NIKKSGGTPT

LLRDNRAERG

AGFVGENAQP

GLGLGFPLPK

ARKRPGRKRR

S SDML VA

K.MAVDLPQO

KI IRQGPKIQ

STDACLFTNO

(NVPDPV FES

SMVPIPGPVG

HKKNCSVRTA

ILENNIGNM~

STSATTTPN4A ILA peptide motif search results -User Parameter$ and Sco-ring noiao [method selected to hnit number of results [expjh!t number.

number of r esu Its requested 30 I HLA molecule type selected -A11)01~ I ~lengthi selected for subsequences to be scored_____ I echoing mode selece forF inut sequence echoing format t[numbere lines; length of user's input peptide sequence nmero sbeuncscores calculated .1 496 inumber of tp-scoring subsequences reporte back inscoring outu ta -29, TABLE 9A Scoring Rtesults Rank Start Position! Sub..quence Residue Sc ore (Estimate of Half'a amfl of Disassociation of Listing a Molecule Cpataining This Subsequence) 126 'ELY GMSSKNIKK [-2.400 IF2 17 4 AYYQSFTF 1. 200 394[ 494 4 :f 1.000 NVG;NRTLRR 0.800 3[ 245 TNEG 0.480 r~r 456PGGGR 0.400 .7 4 58- £SDWFYEK _0.360- I KEOPTELO 0.360 CLRARK _____0.3001 133TLRRRIVKf (F OT~0 VTiF'. 176 YCEVTKNK f0.200 F__T7 3Y. L5 MTQPPIG1 I_7_ .20 F_T_ ARKRRGRK 0.200 7 14 RENHNNNKK 10.180 rT7[ 41 SLCTGDIKR {0.160 rT 4 210. ETNQTNKDK 10.150 I7 DLPQDISNK[ 0.120 07 77L. 0.100 409 jl GSL.CTOI[ 0.090, 38 LLRDNRAER __0.080 1 7 ILENIGNR 0.00 27 _r TGVVPWWEK 26 139 ~3G.O 0.060 L77i~i7L~SEENQTNK:[ ~000___ FPLMSHPSIR 0L 129:1 178 i[ QEFTKNKVK 6 179- .6 Echoed User Peptide Sequence (length =504 residues) ME ELVH4DLVS S YN VHP WET KRRPS S NLNN

ISNKRTMTQF

DEGVVLESEE

EGRQGDDZQS

I LTGSFPLMS N K P.'VHVS PD S RQTSMHLGS LQNMGWT PGS

GKSA

ALEESSEQAR

GHCLSEGSDS

NVRGKRPLWH

PEGCRDQCMD

TNQTNKDKME

DWFYEKESCC

HPSRRGFQAR

S HHH OHW FS P LCTGDI KRRR GLGRDGKG1S

G;GFAE-TGOPIS

SLEEPSKDYR

ESD A V DN VG

SDRAYQYQEF

CEEQKVSDEL

ACGITGWVPW

LSRLJIGMSSK

GARTEHDQHQ

KAAPLPGPTT

EPIQAMQRPl( RSISCPLKRQ ARKRRGRKRR ENHNNNKKDH SOSDDQMLVA NRTLRRRRXV KRMAVDLPOD T1QJKVKK.KL KIIRQGPKIQ MSESOSSSLS STDAGLFTND WEKEDPTELD KNVPDPVFES NIKKSGGTPT SMVPIPGPVG LLRDNRAERG KKKNCSVRTA AGFVGENAQP ILENNIGNRM~ GLGLGFPLPK SrSATTTPNA TABLE 9B LILA peptide motif search results User Paramneter 6n ring*Information ethod selected to limit numEF of ResUt-s :I icit number nrnbcroresults requested F -RO.A molecul@e type selected lIength selectedfor subsequences to be scorcd 1 e-choing mode selected for inpu sqence- F echoing format F numbered lines length *of 'user 's input peptide sequence 71-50 number of subsequence scores calculated Inumber o top-scoring subsequences reported back in scoring output tae[ TABLE 1OA s-corinig esults .k 1 Start Fito S equ ece esiani coe smate of ffiube of DiaSOCiation Of Rankltr .oiin Listing 2 Molecule Containing ThisSubsequence) 173 RAYQOv(EFTK_ 4w- 2.400 277 747: ITGVvPWWICK2.M 9 QARkRRGRK 1-.8900 189_ irlR~P 1.200 180 175V R 0.500 fQE KNVKI _.306 34 v?lPgPVGNK If00 493SATTtPNAG 493 00 7 1SC'PLkRQARK 209 13[ 171 QEETkNKVKK __f0.120 T4.-7-8 OYREAn4NNI< .2 ITY -QLLRdNRAE iI. 120 f1~ 22 1 FA~tGD;HSR 133 rLRR~.0.8 19 71 _SLE~pSY 17 07 NLNNnVGiR ALEEsSEQ Ad..008 ~23 TMTQpI'ECCRk 4 F2- 16

__EQ

1<N~kK~Kiiiiiiiiiiiiiiiiiiiiiii0.060 I26 :1 383 RG ~0.06 FPLMSHPSRR7 0.040 NVGNrTLRP Ii NVRGk LWK I. KE77r._ Echoed User Peptide Sequence (length 504 residues) 1 tMEELVHOLVS ALE~sszop GGFAETCDHS tRSISCPLKRQ AnKRRGRKRR 51 SYNV1U12PWET GHlCLSZ(CS!D SLEEPSKOYR ENIHNNNKKOR

SDSDDQMLVA

101 KRR.PSSNLNN NVRGKRPLWH ESDFAVDNVG NRTLRRRRKV

KRMAVDLPQD

151 ISNKRTMT0? ?Pc.CRCDOD soRAyQYQZF TKNKVKKRKL

XIIRQIGFKIQ

201 DECVVLESEE TNQTNKDKMr CFZQKVSOEL MSESOSSSLS

STDAGLFTND

251 EGROGDOEQS DWFYEKESGG ACGITGWVPW WIEKWDPTELD KNVPi'DvFESq ILTGSFPI-MS HPSRGt'QAR LSRLHGMSSK NIKKSGGTP'r

SM.VPIPGPVG

351 NK:UNVHFSPD SHHHDH1WFS2 GARTERHDOHO LLRDNRAERG

HKKNCSVRTA

401 SR0TSMHILGS LCTGDIKRRR KAA2LPGPTT AGFVGENAQP

ILENNIGNRM

451 LQNMGWTPGS GLGRDGKGIS EpIQA4ORPK GLGLGFPLPK STSATTTpNA 501 GKSA TABLE lOB lILA peptide motif search results -user Parameters; andi Sco-ring InlormatiUn method selected to lumit number of results exlctnmr number of results rcquested LAmoecletype selected A24 length selected for subsequenices to be scored9 echoing mode selectedfor input sequence_______ echoing format nuxrird lines length of usefs, input peptide sequence I number ofsubsequence scores calculated 496Z Innb~otp-scoring subsequences reported back in scoring output tablF.L 0 1 TABLE IIA I Scoring Results Ranj SartPostio Susequence Residue Score (Estimate of Half Iime of Disassociation of [ak, Str Listing a Molecule Containing ThisSubsequence) [TT[ 316 F-QAP.LSRL 30.000 F-711 9 8 L..._KiQDECWVL 14.400_ RTEIIDQiQL I I KNKVK.KRiL. 8.00 CK71 F7- __FE SGGA 700 7200J SI LTCS L 74 Q4 PKGL 6.000 IND- RL SD oo 12 10 R GWHESD[ 6.000J 23 MSESDSSSL IF 777: 6.000 L7~7I ~VGENAQFIL 6.000.-- [T~~i7~I77] LSEGSDSSL L [1 71.NML 9L 6.000 4LVFDLVSAL r5.760 I7~F. 29 HR !S C PL 5.600 TTL .0 7f[-T 478 P LGG 4--I ~~476 K .800..

F-3 40 ASRQTSMHL S8 :1 LSSTUAGL 4.000.. 6 -1 43 1 TSMHLCSL 4.000 F7F F1. 143 SSF-OARGGF 3 .00099 2.592 Echoed User Peptide Sequence (length 504 residues) I MEELVHDLVS ALEESSEOAR GGFAETGOHS RSISCPLKRQ ARKRRCRKRR 51 SYNVHXPWET GHCLS7E-SDS SLEEPSKDYR ENHNNNKKDH SOSODOMLVA 101 1<RRPSSNLNN NVRGKRLWH ESDFAVONVG NRTLRRRRKV KRMAVOLF'QD i51 !SNKRTMTQP PEGCRDOCMD fSDRAYQYQEF TKNZ(VKKCRKL KIIRQ0GPI10 2 ul DEGVVLESEE TNOTNKDKHE CEEQKVSDEL HSESDSSSLS STOAGLFTNJ0 2?51 EGRQGDDEQS ICWFYEKESGG ACGITGVVPW WEKEOPTELD KNVPDPVFES 301 ILTGSFPLMS HPSRRGFQAR LSRLHGNSSK NIKKSGGTPT SMVPIPGPVG 351 NKRMVi4FSPO SHHHDXH4ESP CARTEHDQHQ LLRDNRAERG HI(JNCSVRTA 401 SRQTSMHLGS LCTGCDKRRR KAAPLPGPTT AGFVGENAOP ILENNIGNRM 451 LQNMGWTPGS GLGROCK0US EPIQAMQRPK GLGLGFPLPK STSATTTPNA 501 GKSA TABLE 1113 104 HLA peptide motif search results F User Parzmeters and Scoring Information I ~~method selected to limit number of results number of results requested. liLA molecule type selectedA2 I ~length selected for subsequences to be scored 0 echoing mode selected for input sequence~*e.......cchoing format ubrdlns V lengt of user's input peptide sequence number of subsequence scores calculated 495 :[number of top-scorng s-ubsequcnces reported back in scoring output tabl TABLE 12A Scoring Results i k-SatPstoiListing a Molecule C taingThis Subsequence) i V F E S i.L T S F 1 8s. 0 0 0- 2 RTE~d()HQLL 4.40 0 F F 174 AY QYqEFTKN F 5 F 4S F GrvG.NAQP 1..9-000

D

51 IF -SNVhHPWET ST25T- F 7A 1 0 RQTSmHtLGSL

.O

RGF08RLSPL -9 FYEKeSGGAC -ELVHdLVSAL 1 F 2 237 L..SSLSzTDAGL 0 299 .SI=tGSVP 7It F. 47_ X1MQP1(GLGL 2-30LM4SEsDSSSL VPDPvFESIL 4.8 43 FV. -n i 4.800 63 CLSEgSDSSL 0 -125 AVDN,,.GNRTL__ V~PSSNL F2T2, IQAf'qRPKGL I- .0 .453iZ NMGWtPGSGL -400 1 99 F- TSqrSHHL 4. 304 292 NVPOpVFESI 3. 26 142 _L RM~LOI__ I 2.82 4 7 I NAOPiLENNI 4EIQi 2 DDeQSE)Wr ~..400 E S S1 qA7.....--2.000 RLHCmSKN Echoed User Peptide Sequence (length =504 residues)

MESLVHDLVS

SYNVHHPWET

K<RR P SSN LNN

ISNKRTMTQP'

DZGVVLESSE

EGRQGODEQS

ILTGSFPLNS

NKRMVHFfSPO saQTSMHLGS LQNMGWT PGS

GKSA

P1.EESSEQAR GGFAETGDHS RSISCP'L.KRQ ARK8RGRKRR GHCLSEGSOS SLEEPSKDYR ENHNNNKKDH

SDSDDOMLVA

NvRG1KRPLWH ESOFAVDNVC~ NRTLRRRRKV

KP.MAVOLPQD

PE-GCRDO.DMD SDRAYQYQEF TKNKVKK1RXL

KIIRQGPKIQ

TNQTNKOiK4E CEEQKVSDEL MSESDSSSLS STDAWGk-rND OWFYEKFSGG ACGITGVVPW WEKEDPTELD

KNVPDPVFES

HPSRRGFQAR LS1RLHGXiSSK NIKKSGGTPT

SMVPIPGPVG

SHHHDHWFSP GAIRTEHiDQHQ LLRONRAERG

HKKNCSVRTA

LCTGDIKRL K.AAPLPGPTT AGFVGENAQP ILENNIGNPR4 GLGRDGKGIS EPIQAM40RPK GLGLGFPLPK STSATTTt'NA TABLE 12B HLA peptide motif search results I ~~~User ParametrsandSoigInomto -mecthod -selected to limit numb"r oresit .jexph citnunbcr: number of results lcngt scecletpeseece _B7 ehigmode seleced for iput sequence econfra numibered lines; length of user's input peptidc sequence I number of subsequence scores calculated nmbrof top-scoring Subsequences reported back in scoring outpu-teta TABLE13A Scoring Results- F~-kStart Poiifsu-bs- uence Residue Score (stimmieolr-ialf zcofDssociation o .an PostiniListing a Molecule CPntaining This Subsequence) 7T7[ -i66 PASRQTSMHL 120.000 F 1 1 HPW ETGHCL 80.000 476 -J**MQRPFG~ 40.000 F- 29~~ [S-ISP 40.000___ 114. ONRPGL 36.60 4 LVDVA 000 F_6_1 L VSAL- 20.000 F 7 1-KALL -7 0 F7N AKRFxSL- 423 APLPGPTTA [6.000 014-7 54 GWTPGSGL 6-00d' IND F-IF SVRTASRQT J -5.000 SGPKIOIEG 4.000 [1 I 82 [Y [741 jig" 7L NNVRGKR-PL 4.000 FT717 I f [VT6-7i 40 3 QTS14MLGSL 2!nLSTDAGL 400 DPT8-'1 L K 4.000 I7iL 347 jG GNKRMV 4.000 i2~. I GLGPL 4.000 rFT2-L KRRRKAAPL I4.000 S ~~~ENNIGNRM1L __400.

18 RGGFAET .3.000 _2 23vPDPVF'ES 1. 2.400 13. K~i7L D 2.000 Echoed User Peptide Sequence (length =504 residues) I MEELVHDLVS ALEESSEQAR GGFAETGDHS RSISCPLKRQ ARKRRGRKRR 51 SYNVHHWET GIICLSEGSDS SLEEPSKDYR ENHNNNKKDH SDSDDQMLVA 101 KRRPSSNLNN NVCKRPLW4 ESOFAVDNVG NRTLRRRRKV KRMAVVLPQD 151 ISNKRTMTQP PEGCRDQDMD SDRAYQYQEF TKNKVK1Kt. KTIRQCPKIQ 201 DEGWLZSEE TNQTNKDKM'E CEE01KVSDEL MSESDSSSLS STDAGLFTND 251 EGROCGDDEOS DWFYEKESGG ACGITGVVPW WEKEDPTELD KNVPDPVFES 311 ILTGSFPLMS HPSRRGFOAR LSRLHGM4SSK NU(!(SGGTPT SMVPIPGPVG 351 NKRMVHFSPD SHHHDIHWFSP GARTEHDQHQ LLRONRAERG HKKNCSVRTA 4CI SRQTSMHILGS LCTGDIKRRR-KAAPLPGPTT AGFVGENAQP ILENNIGNRM 451 LONMGWTPGS GLC.RDGKGIS EPIQAM.QRPK GLGLGFPLP( STSATTTPNA 501 GKSA TABLE 13B lILA peptide motif search results User-- a rameters and S~.oring InformatGo mehdslce olmtnumber o f results rqesliLA molecUic Ftype selected- B7 length selected fo; subsequence-s*tobescored--____ echoing rnodc selected for input sequence echoing format Inumere hEis- ~length of use's input pepid sequence i ~iunbe number of subsequence scores calculated. 495 of to R=p-scoring subsequences reported back in scoring output tab ie4-13== TABLE 14A Rank. Start Position: uneRsde Soe(siaeofHl ieo iascaino o a Molecule Containing This Subsequence) 1 18 QARLzRLHGM [30.000 F27 1937 YPOvFEtIL 24.000 V7 L 345. 0.000 3 ViL APIL -20000 125 AVNvGNRTLf T8.000 73 9- 1 V)RrSN f 18.000 TASRqTSMX 12.000 C 475 1 AmORPKGLGL f12.000 53 M 'PSGL 6.000 7. K'~IL 312 PS.RRgF A 13 *42 LPCPtTAGF-V _4.000 103 .__PSSnLNNNV [4.000 109-. ~V1~PL4.000i S. LS~gOSS 4.000 RZFSSTLSRL 4.000 R__rKAAPL 4.000J ;L GIqOEGVV .000 r_21 [997[ ESILtGSFPL 4- 7 2 i74 7 L. RQTSm1ILGSL F2T I LVHdLVSAI, 4L f 2.1 LQDiSNKRT f r-23- 16tT77j RPLWhESDFA~J 00-- 26 1 278 VFt.eKEP L .2.000 LPKStSATTT 4 .0 121 292 j NPVFESI .000 -19 41 iRGRI<rRSYN 2.000 FPLPkSTSAT I- .0- Echoed User Peptide Sequence (length 504 residues) MEELVH DLVS

SYNVHIHPWET

KRRPSSNLNN

I SNNCRTMT02

DEGVVLESEE

EGRQGOOEQS

ILTGSFPLM3 NKR.1VHFSPO

SRQTSMHLGS

LQNMGWTPGS

GKSA

ALEESSrQAR GGFAZIGOHS GHCLSEGSDS SLEEPSKDYR NVRGKRPLWI ESDEAVONVG PEGCRDODMD SDRAYQYQEF TNQTNKDKM1E CEEQKVSDEL DWFYEKESGG ACGITGVVPW HPSRRGFQAR LSRLHGMSSK S1IHVHWFSP -GARTEHOQHQ LCTGOIKRRR KAAPLiPGPTT GLGRDGKGIS EPIQAXQRPK RS ISCPLKRQ

ENHNNNKKOH

NRTLRRRUKV

TKN!<VKI(RKL

MSESOSSSLS

WEKEDPTELD

NIKKSGGTPT

LL.RNRAERG

1 G FVGENAOP

GL.CLGFPLPK

ALRKRRGRKRR

S OSDDQOML VA

KRMAVDLPQD

KIIRQGPKIO

STDAGIAFTND

KN VPOP VFES

SMVPIE'GFVG

HKKNCSVRTA

ILENNIGNRM

STSATTTPNA

TABLE 14B3 lILA peptide motif search results User Parameters andi Scoring Wnormationmethod selected to limit onber of results jexplicit number number ot results requestedt .HLA molecule type selected [3501 leghslce t rsuseuces to be scored econgmd slcedfrinput __unc echoingTornat [numbe lines; length of user's input peptide sequence 504___ nubrof subsequence score-s caculatefnumbe of top-scoring subsequences reported back in SCOnn g output ta-ble- 3 TABLE I Scoring Results Ra nk Start Position. usqec esdeISore (Estimate n Rit Time of Dimasociaton o.f Listing 2 Molecule Containing This Subsequence) TG. HC 40.000 3 h r RPLWHESDF 40.0(0 FGPTTAGF 20.000 1 T- 20.000

FSROTSMHL

f77. 29 1 HSRSISCPL 196 GPIQE V j 12.000 NOI LSsPrDACLF 139 6.000DL -O O N0___ 11 19 KIQDEGVVL

U.V

IRK 9MSHPSRRGF 360 5.000 is:I8 RSYNVHHPW 5.00.

I ru. -I RPSSNI.NN ro ~2Oi 468C!S-,PI0AM 4 GPGKRV4.000- MRASRQTSM 3.000 23 j~93 OPVESIT 1 .000 26 474~ QANRPK .2.00 F-277 W14 1 MAVDLPQDI_________ Echoed User Peptide Sequence (length 504 residues)

MEELVHDLVS

SYNVHHPWET

KRRPSSNLNN

I SNKaTMTQP

DEGVVLESE

EGRQGOOEQS

I LTGSFPLMS NK(RmMFSPD

SRQTSMHLGS

LONMGWT'GS

GKSA

ALEESSEQAR

GHCLSEGSDS

NVRGKRPLWH

PEGCR.QOMD

TNQTNKDKI1E

DWFYEKESGG

HPSRRGFQAR

SHHHDHWFSP

LCTGDIKRRR

GLGRDGKGIS

GGFAETGDHS RSISCPLlKRQ SLEEPSKDYR ENHNNNKKDH ESDFAVONVG NRTLRRRRIKV SDRAYQYQEF TKNKVKKRKL CEEOKVSDEL MSESDSSSLS ACGITGVVPW wEKEDPTELD LSRLI4GMSSK NIKKSGGTPT GARTEHDQHO LLRDNRAERG KAAPLPGPTT AGFVGENAQP EPIOAMQRPK GLGLGFPLPK

ARKJRRGRKRR

S OSD0DQML VA KFNJ4VDLPQD KIIaQGPKIQ ST DAGLETNO Kt4VPDPVFES SMVPI PGPVG k{KKNCSVRTA

ILENNIGNRM

STSATTTFNA

TABLE lILA peptide motif search results User Parameters and Scoring Laformation mefosclectcd to limit number of results {epctwber number of results rcquestcd 3 HLA molecule type selected B 301 length selected for subsequences to be scored 0U ~echoing modc selected for input sequence F _echoing foriat lines_ ____length of uses input pepUde sequence_____ f1 nube ofiS subsequence scores Zcalculd Inme ftop-scoring subsequences reported back in scoring output tabled TABLE 16A Scoring Results Rak Str Position, Subsequence Residue jjScore (Estimate of Half Time of Dis Iassca-on of Ran 1 aLi sting a Molecule Containing This Subsequence) V 7. L.345..__PG?vG 2 0 SLEePSKDY -20.000 [3 96 1__18.000 L_ 3 8 A A~R M 18.00 1 14 ESSEqARGGF f- 10..00- S9.000 0 RPS~nLNN 8.000 F~ r8-1.6 RPLWhESDFA 6.000 r777r VPPvFSIL E SI~GPP 5.000 FTTN 237 SLSsTOAGL 5:.92 17[. 1 KGIePIQA.1 400 F 14 1 5 RWtGICLS 4.000 r'~r~~Zi7:L 2Q iNKRT- j16f 425 m PPTAG FV 4.00 S SDShHHDHW F 3.000 [T1W8 230 .L LMSE:3DSSSL .0 F79- L 553:s RQG E)QSw 3.000 120 9 TASRqTSMHL I.3.000 21 184. KV KLKII .[2.40 T2 1 45NIGNrMLQNM' I~7~f~~i.....RQTSHLGSL____ 2.000 1 SLL]T.9SFPLM 42.000 KSGGtPTSMV 2.000 26 433; FVGEnAQPIL 2.0 J :T 2 F ETNQtNKDKM 00 CLS~~gSDSSL ~2.000 I rI7 F 4 FPLPkSTSAT I 2.000 :TG-T3TF RFaLR .0 Echoed User Peptide Sequence (length =504 residues)

MEELVHDLVS

S YN V H P W

KRRPSSNLN

ISNKIRTMTQP

DEGVVLESEE

EGRQGDDEQS

ILTGSFPLMS

N KR.MVHES P 0

SRQTSMHLGS

LONMGWTPGS

O KSA

ALEESSEQAR

GHCLSEGSOS

NVRCKRPLWH

PEGCRDQOMD

TNQTN!<DKE

DWFYEKESGG

HPSRRGFQAR

SHHHDHWFSP

LCTGDIKRRR

GLGRD(GKGIS

GCFAETG0HS S LEE PS KDYR

ESDFAVDNVG

SORAYQYQEF

CEEOKVS DEL

ACGITGVVPW

LSRLHGMSSK

CARTEHDQHQ

KAkAPLPGPTT

EPIQAMQRPK

RS ISCPLKRQ

ENHNNNKKDH

NRTLRRRRKV

TKNKVKKRKL

MS ESOS S SLS

WEKEOPTELD

NIK!KSGGTPT

LLRDNRAERG

AG FVGENAO P

GLGLGFPLPK

ARKRRGRKRR

SDSDDOMLVA

KRMAVIJLPQD

KIIRQGPKIO

STflAGtFTNEJ 1KN V POP V ES SMVPI PGPVG

HKKNCSVRTA

ILENNIGN&1

STSATTTPNA

TABLE 16B

Claims (10)

1. A recombinant viral expression vector that comprises a polynucleotide that encodes an 84P2A9 protein, wherein the polynucleotide is: a polynucleotide that encodes the amino acid sequence of SEQ ID NO:2; or a polynucleotide that encodes a sequence that is at least 90% identical to the r amino acid sequence of SEQ ID NO:2; or a polynucleotide comprising SEQ ID NO: 1, wherein T can also be U; or Sa polynucleotide comprising the sequence of SEQ ID NO:1, from nucleotide residue number 165 through nucleotide residue number 1676, wherein T can also be U.

2. The recombinant viral expression vector of claim 1 that encodes the polypeptide sequence of SEQ ID NO:2.

3. The recombinant viral expression vector of claim 1, comprising a polynucleotide comprising SEQ ID NO: 1 from nucleotide residue number 720 through nucleotide residue number 1392.

4. The recombinant viral expression vector of any one of claims 1 to 3, wherein the viral vector is derived from a virus selected from the group consisting of vaccinia, fowlpox, canarypox, adenovirus, influenza, poliovirus, adeno-associated virus, lentivirus, and sindbus virus. The recombinant expression vector of claim 4, wherein the viral vector is derived from canarypox.

6. The recombinant expression vector of claim 4, wherein the viral vector is derived from adenovirus.

7. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and the recombinant expression vector of anyone of claims 1 to 6.

8. The pharmaceutical composition of claim 7, further comprising an adjuvant.

9. A method of generating an immune response in a subject comprising administering to the subject an immunogenic amount of the viral vector of any one of claims I to 6. The method of claim 9, wherein the immune response is activation of a B cell.

11. The method of claim 9, wherein the immune response is activation of a T cell.

12. Use of the viral vector of any one of claims 1 to 6 in the manufacture of a medicament for generating an immune response in a subject. Dated this seventh day of December 2006 Agensys, Inc. Patent Attorneys for the Applicant: F B RICE CO