AU2006200977B2 - Specific binding agents of human angiopoietin-2 - Google Patents

Description

- 1 AUSTRALIA PATENTS ACT 1990 COMPLETE SPECIFICATION FOR A STANDARD PATENT ORIGINAL Name of Applicant/s: Amgen Inc. Actual Inventor/s: Hosung Min and Jonathan Oliner Address for Service is: SHELSTON IP 60 Margaret Street Telephone No: (02) 9777 1111 SYDNEY NSW 2000 Facsimile No. (02) 9241 4666 CCN: 3710000352 Attorney Code: SW Invention Title: SPECIFIC BINDING AGENTS OF HUMAN ANGIOPOIETIN-2 Details of Original Application No. 2002365179 dated 11 Oct 2002 The following statement is a full description of this invention, including the best method of performing it known to me/us: File: 42145AUP03 - la SPECIFIC BINDING AGENTS OF HUMAN ANGIOPOIETIN-2 The present application is a divisional application of Australian Application No. 2002365179, which is incorporated in its entirety herein by reference. This application 5 i claims benefit to U.S. Provisional Application Serial No. 60/328,624 filed October 11, 2001, which is incorporated herein by reference in its entirety. FIELD OF INVENTION The present invention relates to specific binding agents that recognize and bind to angiopoietin-2 (Ang-2). More specifically, the invention relates to the production, 10 diagnostic use, and therapeutic use of the specific binding agents and fragments thereof, which specifically bind Ang-2. BACKGROUND OF THE INVENTION Any discussion of the prior art throughout the specification should in no way be considered as an admission that such prior art is widely known or forms part of common 15 general knowledge in the field. Angiogenesis, the formation of new blood vessels from existing ones, is essential to many physiological and pathological processes. Normally, angiogenesis is tightly regulated by pro- and anti-angiogenic factors, but in the case of diseases such as cancer, ocular neovascular diseases, arthritis, and psoriasis, the process can go awry. Folkman, J., Nat. 20 Med., 1:27-31 (1995). There are a number of diseases known to be associated with deregulated or undesired angiogenesis. Such diseases include, but are not limited to, ocular neovascularisation, such as retinopathies (including diabetic retinopathy), age-related macular degeneration, psoriasis, hemangioblastoma, hemangioma, arteriosclerosis, inflammatory disease, such as a 25 rheumatoid or rheumatic inflammatory disease, especially arthritis (including rheumatoid arthritis), or other chronic inflammatory disorders, such as chronic asthma, arterial or post transplantational atherosclerosis, endometriosis, and neoplastic diseases, for example so called solid tumors and liquid (or hematopoietic) tumors (such as leukemias and lymphomas). Other diseases associated with undesired angiogenesis will be apparent to 301 those skilled in the art. Although many signal transduction systems have been implicated in the regulation of angiogenesis, one of the best-characterized and most endothelial cell-selective systems involves the Tie-2 receptor tyrosine kinase (referred to as "Tie-2" or "Tie-2R" (also referred to as "ORK"); murine Tie-2 is also referred to as "tek") and its ligands, the angiopoietins (Gale, N. W. and Yancopoulos, G. D., Genes Dev. 13:1055-1066 [1999]). There are 4 known angiopoietins; 5 angiopoietin-1 ("Ang-1") through angiopoietin-4 ("Ang-4"). These angiopoietins are also referred to as "Tie-2 ligands". (Davis, S., et al., Cell, 87:1161-1169 [1996]; Grosios, K., et al., Cytogenet Cell Genet, 84:118-120 [1999]; Holash, J., et al., Investigative Ophthalmology & Visual Science, 42:1617-1625 [1999]; Koblizek, T. L, et al., Current Biology, 8:529-532 [1998]; Lin, P., et al., Proc Natl 10 Acad Sci USA, 95:8829-8834 [1998]; Maisonpierre, P. C., etal., Science, 277:55 60 [1997]; Papapetropoulos, A., et al., Lab Invest, 79:213-223 [1999]; Sato, T. N., et al., Nature, 375:70-74 [1998]; Shyu, K. G., et al., Circulation, 98:2081-2087 [1998]; Suri, C., et al., Cell, 87:1171-1180 [1996]; Suri, C., et al., Science, 282:468-471 [1998]; Valenzuela, D. M., et al., Proceedings of the National 15 Academy of Sciences of the USA, 96:1904-1909 [1999]; Witzenbichler, B., et al., J Biol Chem, 273:18514-18521 [1998]). Whereas Ang-1 binding to Tie-2 stimulates receptor phosphorylation in cultured endothelial cells, Ang-2 has been observed to both agonize and antagonize Tie-2 receptor phosphorylation (Davis, S., et al., [1996], supra; Maisonpierre, P.C., et al., [1997], supra; Kim, I., J.H. 20 Kim, et al., Oncogene 19(39): 4549-4552 (2000); Teichert-Kuliszewska, K., P.C. Maisonpierre, et al., Cardiovascular Research 49(3): 659-70 (2001)). The phenotypes of mouse Tie-2 and Ang-1 knockouts are similar and suggest that Ang-1-stimulated Tie-2 phosphorylation mediates remodeling and stabilization of developing vessels in utero through maintenance of endothelial 25 cell-support cell adhesion (Dumont, D. J., et al., Genes & Development, 8:1897 1909 [1994]; Sato, T. N., et al., Nature, 376:70-74 [1995]; Suri, C., et al., [1996], supra). The role of Ang-1 in vessel stabilization is thought to be conserved in the adult, where it is expressed widely and constitutively (Hanahan, D., Science, 277:48-50 [1997]; Zagzag, D., et al., Experimental Neurology, 159:391-400 30 [1999]). In contrast, Ang-2 expression is primarily limited to sites of vascular remodeling, where it is thought to block Ang-1 function, thereby inducing a state 2 of vascular plasticity conducive to angiogenesis (Hanahan, D., [1997], supra; Holash, J., et al., Science, 284:1994-1998 [1999]; Maisonpierre, P. C., et al., [1997], supra). Numerous published studies have purportedly demonstrated vessel 5 selective Ang-2 expression in disease states associated with angiogenesis. These pathological conditions include, for example, psoriasis, macular degeneration, and cancer (Bunone, G., et al., American Journal of Pathology, 155:1967-1976 [1999]; Etoh, T., et al., Cancer Research, 61:2145-2153 [2001]; Hangai, M., et al., Investigative Ophthalmology & Visual Science, 42:1617-1625 [2001]; Holash, 10 J., et al., [1999] supra; Kuroda, K., et al., Journal of Investigative Dennatology, 116:713-720 [2001]; Otani, A., et al., Investigative Ophthalmology & Visual Science, 40:1912-1920 [1999]; Stratmann, A., et al., American Journal of Pathology,153:1459-1466 [1998]; Tanaka, S., et al., J Clin Invest, 103:34-345 [1999]; Yoshida, Y., et al., International Journal of Oncology, 15:1221-1225 15 [1999]; Yuan, K., et al., Journal of Periodontal Research, 35:165-171 [2000]; Zagzag, D., et al., [1999] supra). Most of these studies have focused on cancer, in which many tumor types appear to display vascular Ang-2 expression. In contrast with its expression in pathological angiogenesis, Ang-2 expression in normal tissues is extremely limited (Maisonpierre, P. C., et al., [1997], supra; Mezquita, 20 J., et al., Biochemical and Biophysical Research Communications, 260:492-498 [1999]). In the normal adult; the three main sites of angiogenesis are the ovary, placenta, and uterus; these are the primary tissues in normal (i.e., non-cancerous) tissues in which Ang-2 mRNA has been detected. Certain functional studies'suggest that Ang-2 may be involved in tumor 25 angiogenesis. Ahmad et al. (Cancer Res., 61:1255-1259 [2001]) describe Ang-2 over-expression and show that it is purportedly associated with an increase in tumor growth in a mouse xenograft model. See also Etoh et al., supra, and Tanaka et al., supra, wherein data is presented purportedly associating Ang-2 over expression with tumor hypervascularity. However, in contrast, Yu et al. (Am. J. 30 Path., 158:563-570 [2001]) report data to show that overexpression of Ang-2 in 3 Lewis lung carcinoma and TA3 mammary carcinoma cells purportedly prolonged the survival of mice injected with the corresponding transfectants. In the past few years, various publications have suggested Ang-1, Ang-2 and/or Tie-2 as a possible target for anticancer therapy. For example, U.S. Patent 5 Nos. 6,166,185, 5,650,490, and 5,814,464 each disclose the concept of anti-Tie-2 ligand antibodies and receptor bodies. Lin et al. (Proc. Natl. Acad. Sci USA, 95:8829-8834 [1998]) injected an adenovirus expressing soluble Tie-2 into mice; the soluble Tie-2 purportedly decreased the number and size of the tumors developed by the mice. In a related study, Lin et al (J. Clin. Invest., 100:2072 10 2078 [1997]) injected a soluble form of Tie-2 into rats; this compound purportedly reduced tumor size in the rats. Siemeister et al. (Cancer Res., 59:3185-3189 [1999]) generated human melanoma cell lines expressing the extracellular domain of Tie-2, injected these cell lines into nude mice, and concluded that soluble Tie-2 purportedly resulted in a "significant inhibition" of tumor growth and tumor 15 angiogenesis. In view of this information, and given that both Ang-1 and Ang-2 bind to Tie-2, it is not clear from these studies whether Ang-1, Ang-2, or Tie-2 would be an attractive target for anti-cancer therapy. The fusion of certain peptides to a stable plasma protein such as an Ig constant region to improve the half-life of these molecules has been described in, 20 for example, PCT publication WO 00/247 82, published May 4, 2000. The fusion of a protein or fragment thereof to a stable plasma protein such as an Ig constant region to improve the half-life of these molecules has been variously described (see, for example, U.S. Patent 5,480,981; Zheng et al., J. Imunnol., 154:5590-5600, (1995); Fisher et al., N. Engl. J. Med., 334:1697-1702, 25 (1996); Van Zee, K. et al., J. Inununol., 156:2221-2230, (1996); U.S. Patent 5,808,029, issued September 15, 1998; Capon et al., Nature, 337:525-531, (1989); Harvill et al., lInmunotech., 1:95-105, (1995); WO 97/23614, published July 3, 1997; PCT/US 97/23183, filed December 11, 1997; Linsley, J. Exp. Med., 174:561-569, (1991); WO 95/21258, published August 10, 1995). 30 An effective anti-Ang-2 therapy might benefit a vast population of cancer patients because most solid tumors require neovascularization to grow beyond 1-2 4 -5 millimeters in diameter. Such therapy might have wider application in other angiogenesis associated diseases as well, such as retinopathies, arthritis, and psoriasis. There is an undeveloped need to identify new agents that specifically recognize and bind Ang-2. Such agents would be useful for diagnostic screening and therapeutic 5 intervention in disease states that are associated with Ang-2 activity. Accordingly, in one or more preferred embodiments, the present invention relates to specific binding agents of Ang-2 that modulate Ang-2 activity. Such agents of the present invention take the form of peptibodies, i.e., peptides fused to other molecules such as an Fc domain of an antibody, where the peptide moiety specifically binds to Ang-2. 10 It is an object of the present invention to overcome or ameliorate at least one of the disadvantages of the prior art, or to provide a useful alternative. SUMMARY OF THE INVENTION According to a first aspect, the present invention provides a polypeptide capable of binding Ang-2 comprising an amino acid sequence of the formula: 15 RPe'e'e'ee7G (SEQ ID NO: 73) wherein e 5 is a neutral polar or an acidic amino acid residue; e 7 is a neutral hydrophobic amino acid residue, 20 wherein further e 3 is Y or C, e 4 is E, e 6 is M, or a physiologically acceptable salt thereof. 25 According to a second aspect, the present invention provides a polypeptide capable of binding Ang-2 comprising an amino acid sequence of the formula: fi f4RPf 7 fff' 0 f'Gf'Y 4 f1 5 ff 6 f1 7 ff'Y 9 0 (SEQ ID NO: 74) wherein, 30 f' is a neutral hydrophobic or neutral polar amino acid residue; f 2 is a neutral hydrophobic or neutral polar amino acid residue; f 3 is a neutral polar or acidic amino acid residue; f 4 is a neutral hydrophobic or neutral polar amino acid residue; - 5a f 7 is a neutral polar amino acid residue; f is an acidic amino acid residue; f is a neutral polar or acidic amino acid residue; f' 0 is a neutral hydrophobic amino acid residue; 5 f" is a neutral hydrophobic amino acid residue; f 3 is a neutral hydrophobic or neutral polar amino acid residue; f' 4 is a neutral hydrophobic or neutral polar amino acid residue; f 5 is a neutral polar amino acid residue; f]6 is a neutral polar amino acid residue; 10 f'7 is a neutral polar or acidic amino acid residue; f 18 is a neutral hydrophobic or basic amino acid residue; fe9 is a neutral hydrophobic or neutral polar amino acid residue; and fo is a neutral hydrophobic or neutral polar amino acid residue, or a physiologically acceptable salt thereof. 15 According to a third aspect, the present invention provides a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NO: 3, and SEQ ID NOs: 143, 145, 146 and 148, inclusive, wherein said polypeptide is capable of binding to Ang-2, or a physiologically acceptable salt thereof; Peptide SEQ ID NO: Sequence Conl-l 143 AGGMRPYDGMLGWPNYDVQA Conl-3 145 APGQRPYDGMLGWPTYQRIV Conl-4 146 SGQLRPCEEIFGCGTQNLAL Conl-6 148 GQDLRPCEDMFGCGTKDWYG Conl 3 KRPCEEIFGGCTYQ 20 According to a fourth aspect, the present invention provides a polypeptide capable of binding Ang-2 comprising the amino acid sequence SEQ ID NO: 144, or a physiologically acceptable salt thereof. According to a fifth aspect, the present invention provides a polypeptide capable of 25 binding Ang-2 comprising the amino acid sequence of SEQ ID NO. 147, or a physiologically acceptable salt thereof.

- 5b According to a sixth aspect, the present invention provides a fusion polypeptide comprising at least one polypeptide according to any one of the first, second or third aspects, and a vehicle, wherein said fusion polypeptide is capable of binding to Ang-2, or a physiologically acceptable salt thereof. 5 According to a seventh aspect, the present invention provides a polypeptide having the formula: (X')a-F'-(X 2 )b and multimers thereof, wherein: F1 is a vehicle; 10 X1 and X 2 are each independently selected from -(Ll)c-Pl; -(L')c-P'-(L 2 d_p2. -(L')c-P -(L2 )d-p2 3(L)-P ; and -(L')c-P'-(L 2 )d-p2_( 3 )e-p3 -L4 fp4. 15 wherein one or more of P', P 2 ' p 3 , and P 4 each independently comprise a polypeptide according to any one of the first to seventh aspects; L', L2, L', and L 4 are each independently linkers; and a, b, c, d, e, and f are each independently 0 or 1, provided that at least one of a and b is 1, 20 or a physiologically acceptable salt thereof. According to a eighth aspect, the present invention provides a polynucleotide encoding the polypeptide or fusion polypeptide according to any one of the first to seventh aspects. According to a ninth aspect, the present invention provides a host cell comprising a 25 polynucleotide of the eighth aspect. According to a tenth aspect, the present invention provides a pharmaceutical composition comprising an effective amount of the polypeptide or fusion polypeptide according to any one of the first to seventh aspects, in admixture with a pharmaceutically acceptable carrier thereof. 30 According to an eleventh aspect, the present invention provides a method of inhibiting angiogenesis in a mammal comprising administering to the mammal an effective amount of the polypeptide or fusion polypeptide according to any one of the first to seventh aspets, or the pharmaceutical composition according to the tenth aspect.

- 5c According to a twelfth aspect, the present invention provides a method of treating a tumor or cancer in a mammal comprising administering to the mammal an effective amount of the polypeptide or fusion polypeptide according to any one of the first to seventh aspects, or the pharmaceutical composition according to the tenth aspect. 5 According to a thirteenth aspect, the present invention provides a method of treating at least one of ocular neovascular disease, hemangioblastoma, hemangioma, inflammatory disease, chronic inflammatory disorder, endometriosis, neoplastic disease, bone-related disease, or psoriasis in a mammal comprising administering to the mammal an effective amount of the polypeptide or fusion polypeptide according to any one of the first to seventh 10 aspects, or the pharmaceutical composition according to the tenth aspect. According to a fourteenth aspect, the present invention provides use of the polypeptide or fusion polypeptide according to any one of the first to seventh aspects, for the preparation of a medicament for: inhibiting angiogenesis in a mammal; 15 treating a tumor or cancer in a mammal; or treating at least one of ocular neovascular disease, hemangioblastoma, hemangioma, inflammatory disease, chronic inflammatory disorder, endometriosis, neoplastic disease, bone-related disease, chronic inflammatory disorder, or psoriasis in a mammal. 20 The present invention is directed in one embodiment to peptides (also referred to as polypeptides herein) that bind to Ang-2. Also embodied in the present invention are variants and derivatives of such peptides. In another embodiment, the peptides and variants and derivatives thereof of the present invention are attached to vehicles. 25 Unless the context clearly requires otherwise, throughout the description and the claims, the words "comprise", "comprising", and the like are to be construed in an inclusive sense as opposed to an exclusive or exhaustive sense; that is to say, in the sense of "including, but not limited to". In another embodiment, the peptides may be fused to Fc domains, thereby providing 30 peptibodies. Optionally, the peptibodies comprise at least one peptide of, for example, SEQ ID NO:3 - SEQ ID NO:6, or SEQ ID NO:76 - SEQ ID NO: 157, as well as variants and derivatives thereof. Further, the peptides may comprise at least one peptide according to the formulae set forth in SEQ ID NO:65 - SEQ ID NO:75, and SEQ ID NO: 158.

- 5d In yet another embodiment, the invention provides nucleic acid molecules encoding the specific binding agents, and variants and derivatives thereof. In still another embodiment, the invention provides nucleic acid molecules encoding the peptibodies, as well as variants and derivatives thereof. Optionally, such nucleic acid 5 molecules include SEQ ID NO: 33 - SEQ ID NO:53.

In still another embodiment, the invention provides a method of decreasing a tumor by administering an effective amount of the specific binding agents of the present invention to a subject in need thereof. The invention also provides a method of inhibiting angiogenesis in a subject, comprising 5 administering an effective amount of the specific binding agents of the present invention to a subject in need thereof. The invention further provides a method of treating cancer in a subject, comprising an effective amount of the specific binding agents of the present invention to a subject in need thereof. The invention also relates to a polypeptide capable of binding Ang-2 10 wherein the polypeptide comprises the amino -acid sequence WDPWT (SEQ ID NO: 65), and wherein the polypeptide is from 5 to 50 amino acids in length, as well as physiologically acceptable salts thereof. The polypeptide can also comprise the amino acid sequence: WDPWTC 15 (SEQ ID NO: 66) and physiologically acceptable salts thereof. Additionally, the polypeptide can comprise the amino acid sequence: Cz 2 WDPWT (SEQ ID NO: 67). 20 wherein z2 is an acidic or neutral polar amino acid residue, and physiologically acceptable salts thereof. The polypeptide can further comprise the amino acid sequence: Cz 2 WDPWTC (SEQ ID NO: 68) 25 wherein z 2 is an acidic or neutral polar amino acid residue, and physiologically acceptable salts thereof. In another embodiment, the invention relates to a polypeptide capable of binding Ang-2 comprising an amino acid sequence of the formula: ala 2 a3CaSWDPWTCa12a13a14 30 (SEQ ID NO: 69) 6 -7 wherein: a , a 2 , and a are each independently amino acid residues; a5 is an amino acid residue; a is absent or an amino acid residue; 5 a' 3 is absent or a neutral hydrophobic, neutral polar, or a basic amino acid residue; a14 is a neutral hydrophobic or neutral polar amino acid residue; or a physiologically acceptable salt thereof. In a preferred embodiment: a' is V, I, P, W, G, S, Q, N, E, K, R, or H; a2 is V, P, M, G, S, Q, D, E, K, R, or H; 10 a' is A, V, P, M, F, T, G, D, E, K, or H; a 5 is A, V, G, Q, N, D, or E; a" is S, Q, N, D, E, K, or R; a13 is L, T, or H; and a14 is V, L, I, W, or M. 15 In a more preferred embodiment, a' is Q; a 2 is E; a 3 is E; a 5 is D or E; a' 2 is D or E; a 3 is H; and a' 4 is M. It will be appreciated that the use of lower case letters with superscripted numbers herein (such as a' and b') are intended to identify amino acid positions, and are not meant to indicate the single letter abbreviations for a given amino acid. Single letter amino acid 20 abbreviations are given in upper case letters herein. The invention further relates to a polypeptide capable of binding Ang-2 comprising an amino acid sequence of the formula: bib 2 b 3 b 4 b 5 b 6 Cb 8 WDPWTCb"bi 6 b' 7 b1 8 b' 9 b 2 0 25 (SEQ ID NO: 70) wherein: b' is absent or an amino acid residue; b 2 is absent or a neutral hydrophobic, neutral polar, or a basic amino acid residue; b 3 , b 4 , b 5 , and b 6 are each independently absent or amino acid residues; 30 b 8 is an amino acid residue; b 15 is absent or an amino acid residue; b' 6 is absent or a neutral hydrophobic, neutral polar, or a basic amino acid residue; 5 b' is absent or a neutral hydrophobic or neutral polar amino acid residue; b1 8 , b' 9 , and b 20 are each independently absent or amino acid residues; and physiologically acceptable salts thereof. In a preferred embodiment: bl is absent, or A, V, L, P, W, F, T, G, S, Q, N, K, R, or H; b 2is absent, or A, V, L, I, P, W, M, T, G, S, Y, N, K, R, or H; 10 b is absent, or A, L, I, P, W, M, T, G, S, Q, N, E, R, or H; b'is V, I, P, W, G, S, Q, N, E, K, R, or H; b'is V, P, M, G, S, Q, D, E, K, R, or H; b 6 is A, V, P, M, F, T, G, D, E, K, or H; b is- A, V, G, Q, N, D, or E; 15 b" is S, Q, N, D, E, K, or R; b 1 6 is L, T, or H; b1 7 is V, L, I, W, or M; b' 8 is absent, or A, V, L, P, W, F, T, G, Y, Q, D, E, or R; b19 is absent, or V, L, I, P, T, G, S, Y, Q, N, D, E, or R; and 20 b20 is absent, or V, L, P, W, M, T, G, S, Y, Q, N, D, K, or R. In a more preferred embodiment, bl is absent, or P, or T; b 2 is absent, or I, or N; b3 is absent, or R, or I; b 4 is Q; b 5 is E; b 6 is E; ba is D or E; b 15 is D or E; b' 6 is H; b1 7 is M; b" is absent, or W, or P; -b 19 is absent, or G, or E; and b 20 is absent, or V, or K. 25 It will also be appreciated that the invention preferably relates to a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NO: 4, and SEQ ID NO: 76 to SEQ ID NO: 118, inclusive, wherein the polypeptide is capable of binding to Ang-2, as well as physiologically acceptable salts thereof. The peptide sequences are set forth below: 30 TABLE 1 8 PEPTIDE SEQ ID NO. PEPTIDE SEQUENCE Con4-44 76 PIRQEECDWDPWTCEHIMWEV Con4-40 77 TNIQEECEWDPWTCDHMPGK Con4-4 78 WYEQDACEWDPWTCEHMAIEV Con4-31 79 NRLQEVCEWDPWTCEHMENV Con4-C5 80 AATQEECEWDPWTCEHMPRS Con4-42 81 LRHQEGCEWDPWTCEHMFDW Con4-35 82 VPRQKDCEWDPWTCEHMYVG Con4-43 83 SISHEECEWDPWTCEHMQVG Con4-49 84 WAAQEECEWDPWTCEHMGRM Con4-27 85 TWPQDKCEWDPWTCEHMGST Con4-48 86 GHSQEECGWDPWTCEHMGTS Con4-46 87 QHWQEECEWDPWTCDHMPSK Con4-41 88 NVRQEKCEWDPWTCEHMPVR Con4-36 89 KSGQVECNWDPWTCEHMPRN Con4-34 90 VKTQEHCDWDPWTCEHMREW Con4-28 91 AWGQEGCDWDPWTCEHMLPM Con4-39 92 PVNQEDCEWDPWTCEHMPPM Con4-25 93 RAPQEDCEWDPWTCAHMDIK Con4-50 94 HGQNMECEWDPWTCEHMFRY Con4-38 95 PRLQEECVWDPWTCEHMPLR Con4-29 96 RTTQEKCEWDPWTCEHMESQ Con4-47 97 QTSQEDCVWDPWTCDHMVSS Con4-20 98 QVIGRPCEWDPWTCEHLEGL Con4-45 99 WAQQEECAWDPWTCDHMVGL Con4-37 100 LPGQEDCEWDPWTCEHMVRS Con4-33 101 PMNQVECDWDPWTCEHMPRS AC2-Con4 102 FGWSHGCEWDPWTCEHMGST Con4-32 103 KSTQDDCDWDPWTCEHMVGP Con4-17 104 GPRISTCQWDPWTCEHMDQL Con4-8 105 STIGDMCEWDPWTCAHMQVD AC4-Con4 106 VLGGQGCEWDPWTCRLLQGW Con4-1 107 VLGGQGCQWDPWTCSHLEDG 9 Con4-C1. 108 TTIGSMCEWDPWTCAH [QGG Con4-21 109 TKGKSVCQWDPWTCSHVQSG Con4-C2 110 TTIGSMCQWDPWTCAHMQGG Con4-18 111 WVNEVVCEWDPWTCNWDTP Con4-19 112 Q CQWDPWTCKHMRLQ Con4-16 113 AVGSQTCEWDPWTCABLVEV Con4-11 114QGMKFCEWDPWTCA VYR Con4-C4 115TGSMCQWDPWTCEHQGG Con4-23 116 TSQRVGCEWDPWTCQITYT Con4-15 117 QWSWPPCEWDPWTCQTVWPS Con4-9 118 GTSPSFCQWDPWTCSHVQG TN Con4 4TTI QEECEWDPWTCEHM *It will be appreciated that certain peptides and/or peptibodies may contain the prefix "TN", "TN8", or "TN12", and that this prefix may or may not be present for a given Peptibody. Thus, for example, the terms 'TNS-Con4" and 5 "Con4" are used interchangeably herein. In another embodiment, the invention relates to a composition of matter having the formula: 10 and multimers thereof, wherein: F1 is a vehicle;

X

1 and X 2 are each independently selected from 15 ~~-(L')c-P 1 -(IJ)dp 2

(

3 ; P~ n is -1L' )cP'( 2 )dp 2 (L)p 3 ;and)P wherein one or more of P', P', P', and p4 each independently comprise a polypeptide as described herein. For example, in a preferred embodiment, P', p 2 , p 3 , and p 4 can each independently comprise a polypeptide of 10 - 11 SEQ ID NO: 3 to SEQ ID NO: 6, and/or SEQ ID NO: 76 to SEQ ID NO: 157. In another embodiment, the composition of matter is of the formulae: X'-F1 or 5 F1-X2 or a physiologically acceptable salt thereof, where X', F', and X 2 are as defined herein. In another embodiment, the composition of matter is of the formula: F 1-(L I)c-P) or a physiologically acceptable salt thereof, where L', F', and P' are as defined herein. In 10 yet another embodiment, the composition of matter is of the formula: F'-(L')cP'-(L2 d-p2 or a physiologically acceptable salt thereof, where L', F', P1 , P 2 , and c and d are as defined herein. In still another embodiment the composition of matter is of the formula: P'-(L')c-F-(L2 )d-p 2 15 or a physiologically acceptable salt thereof. In a preferred embodiment, F' is an Fe domain or fragment thereof. The invention further relates to a polypeptide capable of binding Ang-2 comprising an amino acid sequence of the formula: PC2 4AC 678' Pc2Dc Lc c c LV 20 (SEQ ID NO: 71) wherein c2 is a neutral hydrophobic amino acid residue; c 4 is a A, D, or E; c6 is an acidic amino acid residue; 25 c 7 is an amino acid residue; and c8 is a neutral hydrophobic, neutral polar, or basic amino acid residue; or a physiologically acceptable salt thereof. In a preferred embodiment, c2 is L or M. In another preferred embodiment, c 6 is D or E.

The invention further relates to a polypeptide capable of binding Ang-2 comprising an amino acid sequence of the formula: d'd 2 d 3 d 4 Pd 6 Dd 8 Ld 1 "dud 1 2 LY d' 5 d' 6 d1 7 d' 8 d' 9 d 2 od0 d 22 (SEQ ID NO: 72) 5 wherein, d' is absent, or an amino acid residue; d2 is absent, or a neutral polar, acidic, or a basic amino acid residue; d is absent, or a neutral hydrophobic or neutral polar amino acid residue; d 4 is absent, or an amino acid residue; 10 d 6 is a neutral hydrophobic amino acid residue; d 8 is a A, D, or E; d' 0 is an acidic amino acid residue; d" is an amino acid residue; d12is a neutral hydrophobic, neutral polar, or basic amino acid residue; 15 d1 5 is absent, or a neutral polar, acidic, or a basic amino acid residue; d16 is absent, or a neutral polar, acidic, or a basic amino acid residue;. d1 7 is absent, or a neutral hydrophobic, or neutral polar amino acid residue; d's is absent, or a neutral hydrophobic, or neutral polar amino acid residue; d'is absent, or a neutral hydrophobic, neutral polar, or basic amino acid 20 residue; d 20 is absent, or an amino acid residue; d' is absent, or a neutral polar, acidic, or a basic amino acid residue; d22is absent, or a neutral hydrophobic, neutral polar, or basic amino acid residue; 25 and physiologically acceptable salts thereof. In a preferred embodiment: d' is T, S, Q, R, or H; d 2 is T, Q, N, or K; d 3 is F; d 4 is M, Q, E, or K; 30 d6 isLorM; 12 ds is D or E; d'" is E; d" is Q or E; d1 is T or R; 5 d' Y, D, E, or K; d' is Q; d 7 is W or F; d'" is L, I, M, or T; d 19 is L, F, or Y; 10 d 20 is Q, D, or E; d 2 l is absent, Q, or H; d22 is absent, A, L, G, S, or R. In a preferred embodiment, the polypeptide comprises at least one amino acid sequence selected from the group consisting of SEQ ID NO: 6, and SEQ ID 15 NO: 119 to SEQ ID NO: 142, inclusive, wherein the polypeptide is capable of binding to Ang-2. SEQ ID NO: 6, and SEQ ID NOS: 119-142 are set forth below: 13 Peptide SEQ ID NO. Peptide Sequence Li1- 119 QNYKPLDELDATLYEHFIFHYT L1-2 120 LNFTPLDELEQTLYEQWTLQQS L1-3 121 TKFNPLDELEQTLYEQWTLQHQ L1-4 122 VKFKPLDALEQTLYEHWMFQQA L-5 123 VKYKPLDELDEILYEQQTFQER L-7 124 TNFMPMDDLEQRLYEQFILQQG Li-9 125 SKFKPLDELEQTLYEQWTLQHA Li-10 126 QKFQPLDELEQTLYEQFMLQQA Li-11 __127 QNFKPMDELEDTLYKQFLFQHS LI-12 128 YKFTPLDDLEQTLYEQWTLQHV LI-13 129 QEYEPLDELDETLYNQWMFHQR __LI-14 130 SNFMPLDELEQTLYEQFMLQHQ _l-1_ 131. QKYQPLDELDKTLYDQFMLQQG __Ll-16 132 QKFQPLDELEETLYKQWTLQQR _ L1-17 133 VKYKPLDELDEWLYHQFrLHHQ LI-i8 134_ QKFMPLDELDEILYEQFMFQQS _ LI-19 135 QTFQPLDDLEEYLYEQWRlRyH LI-20 136 EDYMPLDALDAQLYEQFILLHG L1-21 137 HTFQPLDELEETLYYQWLYDQL Ll-22 138_ YKFNPMDELEQTLYEEFLFQHA AC6-LI 139 TNYKPLDELDATLYEHWILQHS Li-Cl 140 QKFKPLDELEQTLYEQWTLQQR LI-C2 141 TKFQPLDELDQTLYEQWTLQQR LI-C3 142 TNFQPLDELDQTLYEQWTLQQR Li 6 KFNPLDELEETLYEQFTFQQ 14 - 15 The invention also relates to a polypeptide capable of binding Ang-2 comprising an amino acid sequence of the formula: RPe3 e 4eee7G (SEQ ID NO: 73) 5 wherein e 3 is a neutral polar amino acid residue; e 4 is an acidic amino acid residue; e 5 is a neutral polar or an acidic amino acid residue; e is a neutral hydrophobic amino acid residue; 10 e 7 is a neutral hydrophobic amino acid residue; or a physiologically acceptable salt thereof In a preferred embodiment, e 3 is Y or C. In another preferred embodiment, e 4 is D or E. In still another preferred embodiment, e 6 is I or M. The invention further relates to a polypeptide capable of binding Ang-2 comprising 15 an amino acid sequence of the formula: ffOffRPffeff 4 0 f'Gf 1 3f 4 f 5 fl 6 f1 7 f' 8 f' 9

?

0 (SEQ ID NO: 74) wherein, f' is a neutral hydrophobic or neutral polar amino acid residue; 20 f is a neutral hydrophobic or neutral polar amino acid residue; f is a neutral polar or acidic amino acid residue; f4 is a neutral hydrophobic or neutral polar amino acid residue; f 7 is a neutral polar amino acid residue; f8 is an acidic amino acid residue; 25 f) is a neutral polar or acidic amino acid residue; f 10 is a neutral hydrophobic amino acid residue; is a neutral hydrophobic amino acid residue; f 3 is a neutral hydrophobic or neutral polar amino acid residue; f' 4 is a neutral hydrophobic or neutral polar amino acid residue; 30 f 5 is a neutral polar amino acid residue; f1 6 is a neutral polar amino acid residue; f7 is a neutral polar or acidic amino acid residue; f8is a neutral hydrophobic or basic amino acid residue; f19is a neutral hydrophobic or neutral polar amino acid residue; and f 2 o is a neutral hydrophobic or neutral polar amino acid residue; 5 and physiologically acceptable salts thereof. In a preferred embodiment: fV is S, A, or G; f 2 is G, Q, or P; f 3 is Q, G, or D; 10 f 4 is L, M, or Q; f 7 is C or Y; f 8 is E or D; f 9 is E, G, or D; fl'isIorM; 15 fu isForL; f 1 3 is CorW; f1 4 is G or P; f 15 T or N; f is Q, Y, or K; 20 f7is N, D, or Q; f8 is L, V, W, or R; f1 9 is A, Q, Y, or I; and f 20 is L, A, G, or V. In a more preferred embodiment, the invention relates to a polypeptide 25 comprising at least one amino acid sequence selected from the group consisting of SEQ ID NO: 3, and SEQ ID NO: 143 to SEQ ID NO: 148, inclusive, wherein the polypeptide is capable of binding to Ang-2, and physiologically acceptable salts thereof. SEQ ID NO: 3, and SEQ ID NO: 143 to SEQ ID NO: 148 are as follows. Peptide SEQ ID NO. Sequence 16 Con1-1 143 AGGMRPYDGMLGWPNYDVQA Conl-2 144 QTWDDPCMIHLGPVTWRRCI Conl-3 145 APGQRPYDGMLGWPTYQRIV Conl-4 146 SGQLRPCEEIFGCGTQNLAL Con1-5 147 FGDKRPLECMFGGPIQLCPR Con1-6 148 GQDLRPCEDMFGCGTKDWYG Con1 3 KRPCEEIFGGCTYQ In still another aspect, the invention relates to a polypeptide capable of binding Ang-2 comprising an amino acid sequence of the formula: Cg 2 Gg 4 g"DPFTg 1 0 GCg" 5 (SEQ ID NO: 75) wherein g2 is an acidic amino acid residue; g 4 is a neutral hydrophobic amino acid residue; g 5 is E, D, or Q; 10 g 1 0 is a neutral hydrophobic or neutral polar amino acid residue; g1 3 is an acidic residue; and physiologically acceptable salts thereof. In a preferred embodiment, g 2 is E or D. In another preferred embodiment, g 4 is V or M. In yet another embodiment, g1 0 is F or Q. In still another embodiment, g1 3 is D or E. 15 The invention further relates to a polypeptide capable of binding Ang-2 comprising an amino acid sequence of the formula: h'h 2 h 3 h 4 Ch 6 Gh'h 9 DPFTh1 4 GCh 17 h' 8 h' 9 h 20 (SEQ ID NO: 158) wherein, 20 h' is absent or a neutral hydrophobic, neutral polar, or a basic amino acid residue; h2 is a neutral hydrophobic or neutral polar amino acid residue; h 3 is an acidic amino acid residue; h4 is a neutral hydrophobic or neutral polar amino acid residue; 17 h is an acidic amino acid residue; h8 is a neutral hydrophobic amino acid residue; h 9 is E, D, or Q; h1 4 is a neutral hydrophobic or neutral polar amino acid residue; 5 h 1 is an acidic amino acid residue; h' 8 is a neutral hydrophobic, neutral polar, or a basic amino acid residue; h9 is a neutral hydrophobic or neutral polar amino acid residue; and 10 h 20 is absent or an amino acid residue; and physiologically acceptable salts thereof. In a preferred embodiment, h' is absent, or A, L, M, G, K, or H; h2 is L, F, or Q; 15 h 3 is D or E; h 4 isW or Y; h is D or E; h isVorM; h 14 is F or Q; 20 h17 is D or E; h 18 is M, Y, N, or K; h1 9 is L or Q; and h 20 is absent or M, T, G, S, D, K, or R. 25 In a more preferred embodiment, the invention relates to a polypeptide comprising at least one amino acid sequence selected from the group consisting of SEQ ID NO: 5, or SEQ ID NO: 149 to SEQ ID NO: 157 inclusive, wherein said polypeptide is capable of binding to Ang-2, and physiologically acceptable salts thereof. SEQ ID NO: 5, and SEQ ID NO: 149 to SEQ ID NO: 157 are set forth 30 below. 18 Peptide SEQ ID NO: Sequence 12-9-1 149 GFEYCDGMEDPFTFGCDKQT 12-9-2 150 KLEYCDGMEDPFTQGCDNQS 12-9-3 151 LQEWCEGVEDPFTFGCEKQR 12-9-4 152 AQDYCEGMEDPFTFGCEMQK 12-9-5 153 LLDYCEGVQDPFTFGCENL[D 12-9-6 154 HQEYCEGMEDPFTFGCEYQG 12-9-7 155 MLDYCEGMDDPFTFGCDKQM 12-9-C2 156 LQDYCEGVEDPFTFGCENQR 12-9-C 1 157 LQDYCEGVEDPFTFGCEKQR 12-9 5 FDYCEGVEDPFTFGCDNH In a highly preferred embodiment, the invention relates to a a composition of matter having the formula: 5 (X)q-F1-(X 2 )r and multimers thereof, wherein:

F

1 is a vehicle; X1 and X 2 are each independently selected from -(LIvsP'; 10 -(LI)s-PI-(2)t_p2; -(L) -P-(L 2 ) _P 2

-(L

3 ) -P 3 ; and -(LI),-Pl-(L2)tp2-(L3) _p3-L4),-P4; wherein one or more of PI, P 2 , p 3 , and P 4 each independently comprise a polypeptide selected from the group consisting of: 15 (a) the amino acid sequence WDPWT (SEQ ID NO: 65), wherein said polypeptide is from 5 to 50 amino acids in length; (b) the amino acid sequence WDPWTC (SEQ ID NO: 66); (c) the amino acid sequence Cz2WDPWT (SEQ ID NO: 67), wherein z2 is an acidic or neutral polar amino acid residue; 19 (d) the amino acid sequence Cz 2 WDPWTC (SEQ ID NO: 68), wherein Z2 is an acidic or neutral polar amino acid residue; (e) the amino acid sequence Pc 2 Dc 4 Lc 6 c 7 c 8 LY (SEQ ID NO: 71) wherein c2 is a neutral hydrophobic amino acid residue; c4 is A, D, or E; c 6 is an acidic 5 amino acid residue; c7 is an amino acid residue; and c 8 is a neutral hydrophobic, neutral polar, or basic amino acid residue; (f) the amino acid sequence RPe 3 e 4 e 5 e 6 e 7 G (SEQ ID NO: 73) wherein e 3 is a neutral polar amino acid residue; e 4 is an acidic amino acid residue; e 5 is aa neutral polar or an acidic amino acid residue; e is a neutral hydrophobic amino 10 acid residue;. and e 7 is a neutral hydrophobic amino acid residue; (g) the amino acid sequence Cg 2 Gg 4 gDPFrg 0 GCg" (SEQ ID NO: 75) wherein g 2 is an acidic amino acid residue; g 4 is a neutral hydrophobic amino acid residue; gs is a neutral polar or an acidic amino acid residue; gl 0 is a neutral hydrophobic or neutral polar amino acid residue; and g 13 is an acidic residue; 15 (h) A polypeptide of SEQ ID NO: 1; (i) A polypeptide of SEQ ID NO: 2; and (j) A polypeptide of SEQ ID NO: 7; wherein L', L 2 , L 3 , and L are each independently linkers; and q, r, s, t, u, and v are each independently 0 or 1, provided that at least one of q and r is 1; 20 and physiologically acceptable salts thereof. It will be appreciated that the invention further relates to a fusion polypeptide comprising at least one peptide described as described herein and a vehicle, wherein the fusion polypeptide is capable of binding to Ang-2, and physiologically acceptable salts thereof. In the fusion polypeptide, the vehicle is 25 preferably at least one of an Fe domain, polyethylene glycol, a lipid, a cholesterol group, a carbohydrate, and an oligosaccharide. Other suitable vehicles, such as albumin and the like, will be appreciated by those skilled in the art, and are encompassed within the scope of the invention. One skilled in the art will recognize that various molecules can be inserted 30 into specific binding agent structure. Thus a given molecule can be inserted, for 20 example, between the peptide and vehicle portions of the specific binding agents, or inserted within the peptide portion itself, while retaining the desired activity of specific binding -agent. One can readily insert for example, molecules such as an Fc domain or fragment thereof, polyethylene glycol or other related molecules 5 such as dextran, a fatty acid, a lipid, a cholesterol group, a small carbohydrate, a peptide, a cyotoxic agent, a chemotherapeutic agent, a detectable moiety as described herein (including fluorescent agents, radiolabels such as radioisotopes), an oligosaccharide, oligonucleotide, a polynucleotide, interference (or other) RNA, enzymes, hormones, or the like. Other molecules suitable for insertion in 10 this fashion will be appreciated by those skilled in the art, and are encompassed within the scope of the invention. This includes insertion of, for example, a desired molecule in between two consecutive amino acids, optionally joined by a suitable linker. By way of example, in the Con4(C) peptibody sequence: M-Fc-GGGGGAQQEECEWDPWTCEHMLE (SEQ ID NO:23) 15 one skilled in the art could readily insert a desired molecule between, for example, the two adjacent glutamine ("QQ") residues to achieve a desired structure and/or function, while retaining the ability of the peptide to bind Ang-2. Thus, this sequence could be modified as follows: M-Fc-GGGGGAQ-[molecule]-QEECEWDPWTCEHMLE 20 Suitable linker molecules can be added if desired. It will further be appreciated that the molecule can be inserted in a number of locations on the molecule, including on suitable side chains, between the vehicle and peptide sequence as follows: M-Fc-[molecule]-GGGGGAQQEECEWDPWTCEHML±E 25 or in any other location desired by one skilled in the art. Other suitable embodiments will be apparent to those skilled in the art. 21 In still another embodiment, the invention relates to a polynucleotide encoding the specific binding agents (including, but not limited to peptides and/or peptibodies) of the invention, as described herein. One skilled in the art will appreciate that where the amino acid sequence is known, the corresponding 5 nucleotide sequence(s) can be readily determined using known techniques. See for example Suzuki, D., An Introduction to Genetic Analysis, W.H. Freeman Pub. Co. (1986). Exemplary nucleotide sequences encoding peptides of the invention are set forth below. One skilled in the art will recognize that more than one codon can encode for a given amino acid, and therefore the invention relates to any 10 nucleotide sequence which encodes the peptides and/or peptibodies of the invention. Seq. Exemplary DNA Peptide Id Peptide Sequence Sequence No. ccgatccgtcaggaagraatgcga Con4-44 76 PIRQEECDVDPWTCEHMWEV ctgggacccgtggacctgcgaac acatgtgggaagtt (SEQ ID NO: 159) accaacatccaggaagaatgcga Con4-40 77 TNIQEECEWDPWTCDHMPGK atgggacccgtggacctgcgacc acatgccgggtaaa (SEQ ID NO: 160) tggtacgaacaggacgcttgcga Con4-4 78 WYEQDACEWDPWTCEHMAEV atgggacccgtggacctgcgaac acatggctgaagtt (SEQ ID NO: 161) aaccgtctgcaggaagtttgcgaa Con4-31 79 NRLQEVCEWDPWTCEHMENV tgggacccgtggacctgcgaaca catggaaaacgtt (SEQ 10D NO: 162) gctgctacccaggaagaatgcgra 80 atgggacccgtggacctgcgaac Con4- AATQEECEWDPWTCEB3MPRS acatgccgcgttcc (SEQ 11 C5 NO: 163) ctgcgtcaccaggaaggttgcga 81 atgggacccgtggacctgcgaac Con4-42 LRHQEGCEWDPWTCEHM4FDW acatgttcgactgg (SEQ ID NO: 164) 22 gttccgcgtcagaaagactgcga 82 atgggacccgtggacctgcgaac Con4-35 VPRQKDCEWDPWTCEIEMYVG acatgtacgttggt (SEQ ID NO: 165) tccatctcccacgaagaatgegaa 83 tgggacccgtggacctgcgaaca Con4-43 SISHBECEWDPWTCEHMQVG catgcaggttggt (SEQ ID NO: 360) tgggctgctcaggaagaatgcga 84 atgggatccgtggacttgcgaaca Con4-49 WAAQEECEWDPWTCEHMGRM catgggtcgtatg (SEQ ID NO: 166) acttggccgcaggacaaatgcga 85 atgggatccgtggacttgcgaaca Con4-27 TWPQDKCEWDPWTCEHMGST catgggttctact (SEQ ID NO: 167) ggtcactcccaggaagaatgcgg 86 ttgggacccgtggacctgcgaac Con4-48 GHSQEECGWDPWTCENMGTS acatgggtacgtcc (SEQ ID NO: 168) cagcactggcaggaagaatgcga 87 atgggacccgtggacctgcgacc Con4-46 QHWQEECEWDPWTCDIHMPSK acatgccgtccaaa (SEQ ID NO: 169) aacgttcgtcaggaaaaatgcgaa 88 tgggacccgtggacctgcgaaca Con4-41 NVRQEKCEWDPWTCEHMPVR catgccggttcgt (SEQ ID NO: 170) aaatccggtcaggttgaatgcaac 89 tgggacccgtggacctgcgaaca Con4-36 KSGQVECNWDPWTCEHMPRN catgccgcgtaac (SEQ ID NO: 171) gttaaaacccaggaacactgcga 90 ctgggacccgtggacctgcgaac Con4-34 VKTQEHCDWDPWTCEBMREW acatgcgtgaatgg (SEQ ID NO: 172) gcttggggtcaggaaggttgcga 91 AWGQEGCDWDPWTCEHMLPM ctgggacccgtggacctgcgaac Con4-28 acatgctgccgatg (SEQ ID NO: 173) ccggttaaccaggaagactgcga 92 atgggacccgtggacctgcgaac Con4-39 PVNQEDCEWDPWTCEHM\JIPPM acatgccgccgatg (SEQ ID NO: 174) 23 cgtgetccgcaggaagactgcga 93 atgggacccgtggacctgcgctc Con4-25 RAPQEDCEWDPWTCABMDIK acatggacatcaaa (SEQ ID NO: 175) cacggtcagaacatggaatgcga 94 atgggacccgtggacctgcgaac Con4-50 HGQNMECEWDPWTCEHMIFRY acatgttccgttac (SEQ ID NO: 176) ccgcgtctgcaggaagaatgcgtt 95 tgggacccgtggacctgcgaaca Con4-38 PRLQEECVWDPWTCEBMPLR catgccgctgcgt (SEQ ID NO: 177) cgtaccacccaggaaaaatgcga 96 atgggacccgtggacctgcgaac Con4-29 RTTQEKCEWDPWTCEHMESQ acatggaatcccag (SEQ ID NO: 178) cagacctcccaggaagactgcgtt 97 tgggacccgtggacctgcgacca Con4-47 QTSQEDCVWDPWTCDHMVSS catggtttcctcc (SEQ ID NO: 179) caggttatcggtcgtccgtgcgaa 98 tgggacccgtggacctgcgaaca Con4-20 QVIGRPCEWDPWTCEHLEGL : cctggaaggtctg (SEQ.ID NO: 180) tgggctcagcaggaagaatgcgc 99 ttgggacccgtggacctgcgacc Con4-45 WAQQEECAWDPWTCDHMVGL acatggttggtctg (SEQ ID NO: 181) ctgccgggtcaggaagactgcga 100 atgggacccgtggacctgcgaac Con4-37 LPGQEDCEWDPWTCEHMVRS acatggttcgttcc (SEQ ID NO: 182) ccgatgaaccaggttgaatgcga 101 ctgggacccgtggacctgcgaac Con4-33 PMNQVECDWDPWTCEHMPRS acatgccgcgttcc (SEQ ID NO: 183) ttcggttggtctcacggttgcgaat 102 gggatccgtggacttgcgaacac AC2- FGWSHGCEWDPWTCEHMGST atgggttctacc (SEQ ID Con4 NO: 184) aaatccacccaggacgactgcga 103 ctgggacccgtggacctgcgaac Con4-32 KSTQDDCDWDPWTCEBMVGP acatggttggtccg (SEQ ID NO: 185) 24 ggtccgucgtatcCcacgca 104 tgagacccgtggacctgcgaaca Con4-17 GPRISTCQWDPWTCEHIMDQL catggaccagctg (SEQ ID NO: 186) tccaccatcggtgacatgtgcgraa 105 tgggacccgtggacctgcgctca Con4-8 STIGDMCEWDPWTCAHMQVD catgcaggttgac (SEQ ID _______________________NO: 187) gttctgggtggtcagggttgcgaa 106 tgggacccgtggacctgccgtct AC4- VLGGQGCEWDPWTCRLLQGW ctgcagggttgg (SEQ ID Con4 NO: 188) gttctgggtggtcagggttgccag 107 tgggacccgtggacctgctccca Con4-1 VLGGQGCQWDPWTCSHLEDG cctggaagacggt (SEQ ID NO: 189) accaccatcggttccatgotg~cgaa 108 tgggacccgtggacctgcgctca Con4- TTIGSMCEWDPWTCAHMQGG catgcaggtggt (SEQ ID Cl NO: 190) accaaaggtaaatccgtttgccagr 109 tgggacccgtggacctgctccca Con4-21 TKGKSVCQWDPWTCSHMQSG catgcagtccggt (SEQ ID _______________________NO: 19 1) accaccatcggttccatgtgccagy 110 tgggacccgtggacctgcgctca Con4- TTIGSMCQWDPWTCABMQGG catgcagggtggt (SEQ ID C2 NO: 192) tgggttaacgaagttgtttgcgaat 111gggacccgtggacctgcaaccac Con4-18 WVNEVVCEWDPWTCNHWDTP tgggacaccccg (SEQ ID NO: 193) gttgttcaggttggtatgtgccagt 112 gggacccgtggacctgcaaacac Con4-19 VVQVGMCQWDPWTCKI<IMRLQ atgcgtctgcag (SEQ ID _NO: 194) 25 gctgttggttcccagacctgcgaat 113 gggacccgtggacctgcgctcac Con4-16 AVGSQTCEWDPWTCAHLVEV ctggttgaagtt (SEQ ID NO: 195) cagggtatgaaaatgttctgcgaat 114 gggacccgtggacctgcgctcac Con4-11 QGMKMFCEWDPWTCAHIVYR atcgtttaccgt (SEQ ID NO: 196) accaccatcggttccatgtgccag 115 tgggacccgtggacctgcgaaca Con4- TTIGSMCQWDPWTCEHMQGG catgcagggtggt (SEQ ID C4 NO: 197) acctcccagcgtgttggttgcgaat 116 gggacccgtggacctgccagcac Con4-23 TSQRVGCEWDPWTCQBLTYT ctgacctacacc (SEQ ID NO: 198) cagtggtcctggccgccgtgcga 117 atgggacccgtggacctgccaga Con4-15 QWSWPPCEWDPWTCQTVWPS ccgtttggccgtcc (SEQ ID NO: 199) ggtacctccccgtccttctgccagt 118 gggacccgtggacctgctcccac Con4-9 GTSPSFCQWDPWTCSHMVQG atggttcagggt (SEQ ID NO: 200) caggaagaatgcgaatgggaccc TN8- 4 QEECEWDPWTCEHM atggacttgcgaacacatg Con4 (SEQ ID NO: 201) cagaactacaaaccgctggacga actggacgctaccctgtacgaaca Li-1 119 QNYKPLDELDATLYEHIFHYT cttcatcttccactacacc (SEQ ID NO: 202) ctgaacttcaccccgctggacgaa ctggaacagaccctgtacgaaca Li-2 120 LNFTPLDELEQTLYEQWTLQQS gtggaccctgcagcagtcc (SEQ ID NO: 203) accaaattcaacccgctggacga actggaacagaccctgtacgaac Li-3 121 TKFNPLDELEQTLYEQWTLQHQ agtggaccctgcagcaccag (SEQ ID NO: 204) 26 gttaaattcaaaccgctggacgct ctggaacagaccetgtacgaaca Li-4 122 VKFKPLDALEQTLYEHW1FQQA ctggatgttccagcaggct (SEQ ID NO: 205) gttaaatacaaaccgctggacgaa ctggacgaaatcctgtacgaacag L1-5 123 VKYKPLDELDEILYEQQTFQER cagaccttccaggaacgt (SEQ ID NO: 206) accaacttcatgccgatggacgac ctggaacagcgtctgtacgaaca L1-7 124 TNFMPMDDLEQRLYEQFILQQG gttcatcctgcagcagggt (SEQ ID NO: 207) tccaaattcaaaccgctggacgaa ctggaacagaccctgtacgaaca L1-9 125 SKFKPLDELEQTLYEQWTLQHA gtggaccctgcagcacgct (SEQ ID NO: 208) cagaaattccagccgctggacga actggaacagaccctgtacgaac Li-10 126 QKFQPLDELEQTLYEQFMLQQA agttcatgctgcagcaggct (SEQ ID NO: 209) cagaacttcaaaccgatggacga attggaagacaccctgtacaaaca Li-11 127 QNFKPMDELEDTLYKQFLFQHS gttcctgttccagcactcc (SEQ ID NO: 210) tacaaattcaccccgctggacgac ctggaacagaccctgtacgaaca Li-12 128 YKFTPLDDLEQTLYEQWTLQHV gtggaccctgcagcacgtt (SEQ ID NO: 211) caggaatacgaaccgctggacga actggacgaaaccctgtacaacc L1-13 129 QEYEPLDELDETLYNQWVFHQR agtggatgttccaccagcgt (SEQ ID NO: 212) tccaacttcatgccgctggacgaa ctggaacagaccctgtacgaaca Li-14 130 SNFMPLDELEQTLYEQFMLQHQ gttcatgctgcagcaccag (SEQ ID NO: 213) cagaaataccagccgctggacga actggacaaaaccctgtacgatca Li-15 131 QKYQPLDELDKTLYDQFMLQQG gttcatgctgcagcagggt (SEQ ID NO: 214) 27 cagaaattccagccgctggacga actggaagaaaccctgtacaaac LI-16 132 QKFQPLDELEETLYKQWTLQQR agtggaccctgcagcagcgt (SEQ ID NO: 215) gttaaatacaaaccgctggacgaa ctggacgaatggctgtaccacca L1-17 133 VKYKPLDELDEWLYHQFTLIHIQ gttcaccctgcaccaccag (SEQ ID NO: 216) cagaaattcatgccgctggacgaa ctggacgaaatcctgtacgaacag LI-18 134 QKFMPLDELDEILYEQFMFQQS ttcatgttccagcagtccc (SEQ ID NO: 217) cagaccttccagcgctggacga cctggaagaatacttgtacgaaca Li-19 135 QTFQPLDDLEEYLYEQWIRRYH gtggatccgtcgttaccac (SEQ ID NO: 218) gaagactacatgccgctggacgc tctggacgctcagctgtacgaaca L1-20 136 EDYMPLDALDAQLYEQFILLHG. gttcatcctgctgcacggt (SEQ ID NO: 219) cacaccttccagccgctggacga actggaagaaaccctgtactacca L1-21 137 HTFQPLDELEETLYYQWLYDQL gtggctgtacgaccagctg (SEQ ID NO: 220) tacaaattcaacccgatggacgaa ctggaacagaccctgtacgaaga L1-22 138 YKFNPMDELEQTLYEEFLFQHA attcctgttccagcacgct (SEQ ID NO: 221) accaactacaaaccgctggacga actggacgctaccctgtacgaaca AC6-L1 139 TNYKPLDELDATLYEHWILQHS ctggatcctgcagcactcc (SEQ ID NO: 222) cagaaattcaaaccgctggacga actggaacagaccctgtacgaac Li-Cl 140 QKFKPLDELEQTLYEQWTLQQR agtggaccctgcagcagcgt (SEQ ID NO: 223) accaaattccagccgctggacga actggaccagaccctgtacgaac L1-C2 141 TKFQPLDELDQTLYEQWTLQQR agtggaccctgcagcagcgt (SEQ ID NO: 224) 28 accaacttccagccgctggacga actggaccagaccctgtacgaac L1-C3 142 TNFQPLDELDQTLYEQWTLQQR agtggaccctgcagcagcgt (SEQ ID NO: 225) aaattcaacccgctggacgagctg gaagagactctgtacgaacagttt -LI 6 KFNPLDELEETLYEQFTFQQ acttttcaacag (SEQ ID NO: 226) gctggtggtatgcgtccgtacgac ggtatgctgggttggccgaactac Conl-1 143 AGGMRPYDGMLGWPNYDVQA gacgttcaggct (SEQ ID NO: 227) cagacttcggacgatccgtgcatg cacattctgggtccgrgttacttggc Conl-2 144 QTWDDPCMILGPVTWRRCI gtcgttgcatc (SEQ ID NO: _______228) gctccgggtcagcgtccgtacga cggtatgctgggttggccgaacta Con1-3 145 APGQRPYDGMLGWPTYQRIV ccagcgtatcgtt (SEQ ID NO: 229) tccggtcagctgcgtccgtgcgaa caaatcttcggttgcggttaccag Conl-4 146 SGQLRPCEEIFGCGTQNLAL aacctggctctg (SEQ ID NO: 230) ttcggtgacaaacgtccgctagaa tgcatgttcggtggtccgatccag Con -5 147 FGDKRPLECFGGPIQLCPR ctgtgcccgcgt (SEQ ID NO: 231) ggtcaggacctgcgtccgtgcga agacatttcggttgcggtaccaa Con 1-6 148 GQDLRPCEDMIFGCGTKDWYG agactggtacggt (SEQ ID NO: 232) ggtttcgaatactgcgacggtatg gaatacccgttcaccttcagttgc 12-9-1 149 GFEYCDGNMDPFtFGCDKQT gacaaacagacc (SEQ ID NO: 233) aaactggaatactgcgacggtat gaagacccgttcacccagggttge 12-9-2 150 KLEYCDGMEDPFTQGCDNQS cgacaaccagtcc (SEQ ID NO: 234) 12-9-3 151 LQEWCEGVEDPFTFGCEKQR ctgcaggaatggtgcgaaggtgtt 29 gaagacccgttcaccttcggttgc gaaaaacagcgt (SEQ ID NO: 235) gctcaggactactgcgaaggtatg gaagacccgttcaccttcggttgc 12-9-4 152 AQDYCEGMEDPFTFGCEMQK gaaatgcagaaa (SEQ ID NO: 236) ctgc tggactactgcgaaggtgtt caggacccgttcaccttcggttgc 12-9-5 153 LLDYCEGVQDPFTFGCENLD gaaaacctggac (SEQ ID NO; 237) caccaggaatactgcgaaggtat ggaagacccgttcaccttcggttg 12-9-6 154 HQEYCEGMEDPFTFGCEYQG cgaataccagggt (SEQ.ID NO: 238) atgctggactactgcgaaggtatg gacgacccgttcaccttcggttgc 12-9-7 155 MLDYCEGMDDPFTFGCDKQM gacaaacagatg (SEQ ID NO: 239) ctgcaggactactgcgaaggtgtt gaagacccgttcaccttcggttgc 12-9-C2 156 LQDYCEGVEDPFTFGCENQR gaaaaccagcgt (SEQ lID NO: 240) ctgcaggactactgcgaaggtgtt gaagacccgttcaccttcggttgc 12-9-Cl 157 LQDYCEGVEDPFTFGCEKQR gaaaaacagcgt (SEQ ID NO: 241) ttcgactactgcgaaggtgttgaa 12-9 5 FDYCEGVEDPFTFGCDNH gacccgttcactttcggctgtgata accac (SEQ ID NO: 242) In still another embodiment, the invention relates to expression vectors comprising at least one polynucleotide of the invention. In another embodiment, 5 the invention relates to host cells comprising the expression vector. It will be appreciated that the host cells are preferably prokaryotic cells (such as E. coli cells) or eukaryotic cells. The invention also relates to a pharmaceutical composition comprising an effective amount of a composition as described herein, in admixture with a 10 pharmaceutically acceptable carrier. 30 The invention also relates to a method of inhibiting undesired angiogenesis in a mammal comprising administering a therapeutically effective amount of a polypeptide or composition as described herein. The invention also relates to a method of modulating angiogenesis in a mammal comprising administering a 5 therapeutically effective amount of a polypeptide or composition as described herein. The invention further relates to a method of inhibiting tumor growth characterized by undesired angiogenesis in a mammal comprising administering a therapeutically effective amount of a polypeptide or composition as described herein. Additionally, the invention relates to a method of treating cancer in a 10 mammal comprising administering a therapeutically effective amount of a polypeptide or composition as described herein, and a chemotherapeutic agent. In a preferred embodiment, the chemotherapeutic agent is at least one of 5-FU, CPT-1 1, and Taxotere. It will be appreciated, however, that other suitable chemotherapeutic agents and other cancer therapies can be used. 15 The invention also relates to a method of modulating at least one of vascular permeability or plasma leakage in a mammal comprising administering a therapeutically effective amount of a polypeptide or composition as described herein. The invention further relates to a method of treating at least one of ocular neovascular disease, obesity, hemangioblastoma, hemangioma, arteriosclerosis, 20 inflammatory disease, inflammatory disorders, atherosclerosis, endometriosis, neoplastic disease, bone-related disease, or psoriasis in a mammal comprising administering a therapeutically effective amount of a polypeptide or composition as described herein. It will be appreciated that the specific binding agents of the invention can 25 be used to treat a number of diseases associated with deregulated or undesired angiogenesis. Such diseases include, but are not limited to, ocular neovascularisation, such as retinopathies (including diabetic retinopathy and age related macular degeneration) psoriasis, hemangioblastoma, hemangioma, arteriosclerosis, inflammatory disease, such as a rheumatoid or rheumatic 30 inflammatory disease, especially arthritis (including rheumatoid arthritis), or other chronic inflammatory disorders, such as chronic asthma, arterial or post 31 transplantational atherosclerosis, endometriosis, and neoplastic diseases, for example so-called solid tumors and liquid tumors (such as leukemias). Additional diseases which can be treated by administration of the specific binding agents will be apparent to those skilled in the art. Such additional diseases include, but are 5 not limited to, obesity, vascular permeability, plasma leakage, and bone-related disorders, including osteoporosis. Thus, the invention further relates to methods of treating these diseases associated with deregulated or undesired angiogenesis. Other embodiments of this invention will be readily apparent from the disclosure provided herewith. 10 BRIEF DESCRIPTION OF THE FIGURES Figure 1 depicts a graph of tumor volume (y-axis) versus time (x-axis) in A-431 tumor bearing mice treated with peptibody TN8-Con4-C of the present 15 invention, or with phosphate buffered saline (PBS). Details are described in the Examples. Figure 2 depicts a graph of peptibody concentration (y-axis) versus time post-dose (x-axis) in wildtype mice treated with a 50gg dose of either 2xCon4-C, L1-7-N, or L1-21-N peptibody. Details are described in the Examples. 20 Figure 3 depicts a graph of tumor volume (y-axis) versus time (x-axis) in A431 tumor bearing mice treated with peptibody 2xCon4-C according to the present invention, or with phosphate buffered saline (PBS) or control peptibody. Details are described in the Examples. Figure 4 depicts a graph representing in vitro growth of cultured A431 25 cells treated with peptibody Con4-C according to the present invention, control peptibody, or untreated. Details are described in the Examples. Figure 5 depicts a graph of tumor volume (y-axis) versus time (x-axis) in Colo205 tumor cells treated with peptibody Con4-C, peptibody L1-7-N, peptibody L1-21-N, or peptibody 2xCon4-C according to the present invention, or 30 with phosphate buffered saline (PBS), anti-Ang-2 antibody (Ab536), or Fc. Details are described in the Examples. 32 Figure 6 depicts a graph of tumor volume (y-axis) versus time (x-axis) in Colo205 xenograft tumor bearing mice treated with varying doses of peptibody 2xCon4-C according to the present invention, or with phosphate buffered saline (PBS) or Fc. Details are described in the Examples. 5 Figure 7 depicts a graph of tumor volume (y-axis) versus time (x-axis) in Colo205 xenograft tumor bearing mice treated with peptibody 2xCon4-C according to the present invention, or with control peptibodies. Figure 7 also depicts a graph of CD31 stained area/total tumor area for these peptibodies. Details are described in the Examples. 10 Figure 8 depicts a graph of tumor volume (y-axis) versus time (x-axis) in Colo205 xenograft tumor bearing mice treated with peptibody 2xCon4-C according to the present invention, or with phosphate buffered saline (PBS) or control peptibody. Details are described in the Examples. This graph shows that anti-Ang-2 peptibodies are capable of inhibiting Colo205 tumor growth 15 irrespective of when dosing begins. Figure 9 depicts a summary of complete response (CR) rates obtained in female nude mice using antibody Ab536 or with peptibody 2xCon4-C, in both the A431 and Colo-205 xenograft models. Details are described in the Examples. Figure 10A depicts a graph of tumor volume (y-axis) versus time (x-axis) 20 in Colo205 xenograft tumor bearing mice treated with peptibody 2xCon4-C according to the present invention, or a combination of 2xCon4-C and taxotere, or with phosphate buffered saline (PBS), or with PBS plus taxotere. Details are described in the Examples. Figure 10B depicts a graph of tumor volume (y-axis) versus time (x-axis) 25 in Colo205 xenograft tumor bearing mice treated with peptibody 2xCon4-C according to the present invention, or a combination of 2xCon4-C and 5-FU, or with phosphate buffered saline (PBS), or with PBS plus 5-FU. Details are described in the Examples. Figure 11 A depicts a graph of paw swelling levels (AUC±SE) in an 30 adjuvant-induced arthritis model in rats treated with peptibody 2xCon4-C .according to the present invention, or with phosphate buffered saline (PBS), or 33 with control peptibody, or normal or arthritis controls. Details are described in the Examples. Figure 1 1B depicts a graph of paw. bone mineral density (BMD) in an adjuvant-induced arthritis model in rats treated with peptibody 2xCon4-C 5 according to the present invention, or with phosphate buffered saline (PBS), or with control peptibody, or normal or arthritis controls. Details are described in the Examples. Figure 11C depicts a graph of change in body weight in an adjuvant induced arthritis model in rats treated with peptibody 2xCon4-C according to the 10 present invention, or with phosphate buffered saline (PBS), or with control peptibody, or normal or arthritis controls. Details are described in the Examples. Figure 12 depicts two graphs depicting inhibition of VEGF-induced corneal angiogenesis in rats. The first graph depicts number of blood vessels measured in rats treated with bovine serum albumin (BSA), VEGF plus phosphate 15 buffered saline (PBS), or VEGF plus peptibody Con4-C of the invention. The second graph depicts blood vessel area (mm2) in rats treated with BSA, VEGF plus phosphate buffered saline (PBS), or VEGF plus peptibody Con4-C of the invention. Details are described in the Examples. Figures 13A, 13B, and 13C depict epitope mapping data (O.D. 370) for 20 full-length human Ang-2 (hAng-2), to the N-terminus of hAng-2, and to the C terminus of hAng-2, respectively, for peptibodies TN8-Con4-C, L1-7-N, and 12 9-3-C according to the invention, as well as for control peptibody, Tie2-Fc, C2B8, or 5B 12. Details are described in the Examples. Figure 14 depicts binding affinity (Ko)of the 2xCon-4-C peptibody 25 according to the invention, using the Sapidyne KinExA assay. Details are described in the Examples. DETAILED DESCRIPTION OF INVENTION 34 The section headings are used herein for organizational purposes only, and are not to be construed as ini any way limiting the subject matter described. Standard techniques may be used for recombinant DNA molecule, protein, and antibody production, as well as for tissue culture and cell transformation. 5 Enzymatic reactions and purification techniques are typically performed according to the manufacturer's specifications or as commonly accomplished in the art using conventional procedures such as those set forth in Sambrook et al. (Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY [1989]), or as described herein. Unless specific 10 definitions are provided, the nomenclature utilized in connection with, and the laboratory procedures and techniques of analytical chemistry, synthetic organic chemistry, and medicinal and pharmaceutical chemistry described herein are those well known and commonly used in the art. Standard techniques may be used for chemical syntheses, chemical analyses, pharmaceutical preparation, formulation, 15 and delivery, and treatment of patients. Definitions The terms used throughout this specification are defined as follows, unless otherwise limited in specific instances. 20 The term "Ang-2" refers to the polypeptide set forth in Figure 6 of U.S. Patent No. 6,166,185 ("Tie-2 ligand-2") or fragments thereof as well as related polypeptides which include allelic variants, splice variants, derivatives, substitution, deletions, and/or insertion variants, fusion peptides and polypeptides, and interspecies homologs.. The Ang-2 polypeptide may or may not include 25 additional terminal residues, e.g., leader sequences, targeting sequences, amino terminal methionine, amino terminal methionine and lysine residues, and/or tag or fusion proteins sequences, depending on the manner in which it is prepared. The term "biologically active" when used in relation to Ang-2 or an Ang-2 specific binding agent refers to a peptide or polypeptide having at least one 30 activity characteristic of Ang-2 or of an Ang-2 specific binding agent. A specific 35 binding agent of Ang-2 may have agonist, antagonist, or neutralizing or blocking activity with respect to at least one biological activity of Ang-2. The term "specific binding agent" refers to a molecule, preferably a proteinaceous-molecule, that specifically binds Ang-2, and variants and 5 derivatives thereof, as defined herein. A specific binding agent may be a protein, peptide, nucleic acid, carbohydrate, lipid, or small molecular weight compound which binds preferentially to Ang-2. In a preferred embodiment, the specific binding agent according to the present invention is a peptide or a peptibody, as well as fragments, variants or derivatives thereof, either alone or in combination 10 with other amino acid'sequences, provided by known techniques. Such techniques include, but are not limited to enzymatic cleavage, chemical cleavage, peptide synthesis or recombinant techniques. The anti-Ang-2 specific binding agents of the present invention are capable of binding portions of Ang-2 that modulate, e.g., inhibit or promote, the biological activity of Ang-2 and/or other 15 Ang-2-associated activities. The term "variants," as used herein, include those peptides and polypeptides wherein amino acid residues are inserted into, deleted from and/or substituted into the naturally occurring (or at least a known) amino acid sequence for the binding agent. Variants of the invention include fusion proteins as 20 described below. "Derivatives" include those binding agents that have been chemically modified in some manner distinct from insertion, deletion, or substitution variants. "Specifically binds Ang-2" refers to the ability of a specific binding agent (such as a peptibody, or peptide portion thereof) of the present invention to 25 recognize and bind mature, full-length or partial-length human Ang-2 polypeptide, or an ortholog thereof, such that its affinity (as determined by, e.g., Affinity ELISA or BlAcore assays as described herein) or its neutralization capability (as determined by e.g., Neutralization ELISA assays described herein, or similar assays) is at least 10 times as great, but optionally 50 times as great, 100, 250 or 30 500 times as great, or even at least 1000 times as great as the affinity or neutralization capability of the same for any other angiopoietin or other peptide or 36 polypeptide, wherein the peptide portion of the peptibody is first fused to a human Fc moiety for evaluation in such assay. The term "epitope" refers to that portion of any molecule capable of being recognized by and bound by a specific binding agent, e.g., -a peptibody, at one or 5 more of the binding agent's antigen binding regions. Epitopes usually consist of chemically active surface groupings of molecules, such as for example, amino acids or carbohydrate side chains, and have specific three-dimensional structural characteristics as well as specific charge characteristics. Epitopes as used herein may be contiguous or non-contiguous. 10 The term "inhibiting and/or neutralizing epitope" is an epitope, which when bound by a specific binding agent such as a peptibody, results in the loss of (or at least the decrease in) biological activity of the molecule, cell, or organism containing-such epitope, in vivo, in vitro, or in situ. In the context of the present invention, the neutralizing epitope is located on or is associated with a 15 biologically active region of Ang-2. Alternatively, the term "activating epitope" is an epitope,- which when bound by a specific binding agent of the invention, such as an antibody, results in activation, or at least maintenance of a biologically active conformation, of Ang-2. The term "peptibody fragment" refers to a peptide or polypeptide which 20 comprises less than a complete, intact peptibody. The term "naturally occurring" when used in connection with biological materials such as nucleic acid molecules, polypeptides, host cells, and the like, refers to those which are found in nature and not modified by a human being. The term "isolated" when used in relation to Ang-2 or to a specific binding 25 agent of Ang-2 refers to a compound that is free from at least one contaminating polypeptide or compound that is found in its natural environment, and preferably substantially free from any other contaminating mammalian polypeptides that would interfere with its therapeutic or diagnostic use. The term "mature" when used in relation to Ang-2 peptibody or a 30 fragment thereof, or to any other proteinaceous specific binding agent of Ang-2 refers to a peptide or a polypeptide lacking a leader or signal sequence. When a 37 binding agent of the invention is expressed, for example, in a prokaryotic host cell, the "mature" peptide or polypeptide may also include additional amino acid residues (but still lack a leader sequence) such as an amino terminal methionine, or one or more methionine and lysine residues. A peptide or polypeptide 5 produced in this manner may be utilized with or without these additional amino acid residues having been removed. The terms "effective amount" and "therapeutically effective amount" when used in relation to a specific binding agent of Ang-2 refers to an amount of a specific binding agent that is useful or necessary to support an observable change 10 in the level of one or more biological activities of Ang-2. The change may be either an increase or decrease in the level of Ang-2 activity. Preferably, the change is a decrease in Ang-2 activity. The term "peptibody" refers to a molecule comprising an antibody Fc domain attached to at least one peptide. The production of peptibodies is 15 generally described in PCT publication WO 00/247 82, published May 4, 2000. The term "variants," as used herein, include those molecules such as peptides or peptide-vehicle combinations such as peptibodies of the present invention wherein amino acid residues are inserted into, deleted from and/or substituted into amino acid sequence for such molecules. Variants having one or 20 more amino acids inserted include fusion proteins as described below. "Derivatives" include those peptides and/or peptide-vehicle combinations such as peptibodies that have been chemically modified in some manner distinct from insertion, deletion, or substitution variants. The term "fragment" refers to a peptide or peptide-vehicle combination 25 that comprises less than the full-length amino acid sequence of such peptides and/or peptide-vehicle combinations. Such a fragment may arise, for example, from a truncation at the amino terminus, a truncation at the carboxy-terninus, and/or an internal deletion of a residue(s) from the amino acid sequence. of the peptide or peptide-vehicle combination. Fragments may result from alternative 30 RNA splicing or from in vivo or in vitro protease activity. Such fragments may also be constructed by chemical peptide synthesis methods, or by modifying a 38 polynucleotide encoding a peptide, peptide-vehicle combination, or an Fe portion and/or peptide portion of a peptibody. The term " Fc" refers to one type of vehicle of the present invention, and comprises the sequence of a non-antigen-binding fragment of an antibody 5 resulting from the proteolytic digestion of a whole antibody, whether in monomeric or multimeric form. The source of the Fc in the present invention is preferably fully human Fc, and may be any of. the immunoglobulins, although IgG1 and IgG2 are preferred. However, Fc molecules that are partially human, or obtained from non-human species are also included herein. Fc's are made up of 10 monomeric polypeptides that may be linked into dimeric or multimeric forms by covalent (i.e., disulfide bonds) and non-covalent association. The number of intermolecular disulfide bonds between monomeric subunits of native Fc molecules ranges from I to 4 depending on class (e.g., IgG, IgA, IgE) or subclass (e.g., IgG1, IgG2, IgG3, IgA1, IgGA2). One example of a native Fc is a disulfide 15 bonded dimer resulting from papain digestion of an IgG [see Ellison et al. (1982), Nucl. Acids. Res. 10: 4071-9]. The term "native Fc" as used herein is generic to the monomeric, dimeric, and multimeric forms. The term "Fec domain" encompasses native Fc and Fc variant molecules and sequences as defined above. As with Fc variants and native Fc's, the term "Fc 20 domain" includes molecules in monomeric or multimeric form, whether digested from whole antibody or produced by other means. The term "multimer" as applied to Fe domains or molecules comprising Fc domains refers to riolecules having two or more polypeptide chains associated covalently, noncovalently, or by-both covalent and non-covalent interactions. IgG 25 molecules typically form dimers; IgM, pentamers; IgD, dimers; and IgA, monomers, dimers, trimers, or tetramers. Multimers may be formed by exploiting the sequence and resulting activity of the native Ig source of the Fe or by derivatizing (as defined below) such a native Fc. The term "dimer" as applied to Fe domains or molecules comprising Fe 30 domains refers to molecules having two polypeptide chains associated covalently or non-covalently. 39 The term "vehicle" refers to a molecule that prevents degradation and/or increases half-life, reduces toxicity, reduces immunogenicity, or increases biological activity of a therapeutic protein. Exemplary vehicles include an Fc domain as well as a linear polymer (e.g., polyethylene glycol (PEG), polylysine, 5 dextran, etc.); a branched-chain polymer (See, for example, U.S. Patent No. 4,289,872 to Denkenwalter et al., issued September 15, 1981; U. S. Patent No. 5,229,490 to Tam, issued July 20, 1993; WO 93/21259 by Frechet et aL, published 28 October 1993); a lipid; a cholesterol group (such as a steroid); a carbohydrate or oligosaccharide; or any natural or synthetic protein, polypeptide 10 or peptide that binds to a salvage receptor. Vehicles are further described hereinafter. The terms "derivatizing" and "derivative" or "derivatized" comprise processes and resulting compounds respectively in which (1) the compound has a cyclic portion; for example, cross-linking between cysteinyl residues within the 15 compound; (2) the compound is cross-linked or has a cross-linking site; for example, the compound has a cysteinyl residue and thus forms cross-linked dimers in culture or in vivo; (3) one or more peptidyl linkage is replaced by a non peptidyl linkage; (4) the N-terminus is replaced by -NRR1, NRC(O)Rl, NRC(O)OR1, -NRS(O)2R1, -NHC(O)NHR, a succinimide group, or substituted 20 or unsubstituted benzyloxycarbonyl-NH-, wherein R and R1 and the ring substituents are as defined hereinafter; (5) the C-terminus is replaced by -C(O)R2 or -NR3R4 wherein R2, R3 and R4 are as defined hereinafter; and (6) compounds in which individual amino acid moieties are modified through treatment with agents capable of reacting with selected side chains or terminal residues. 25 Derivatives are further described hereinafter. The term "peptide" refers to molecules of about 3 to about 75 amino acids, with molecules of about 5 to 50 amino acids preferred, 8 to 40 more preferred, and those of about 10 to 25 amino acids most preferred. Peptides may be naturally occurring or artificial (i.e., non-naturally occurring) amino acid sequences. 30 Exemplary peptides may be generated by any of the methods set forth herein, such as carried in a peptide library (e.g., a phage display library), generated by 40 chemical synthesis, derived by digestion of proteins, or generated using recombinant DNA techniques. The term "pharmacologically active" means that a substance so described is determined to have activity that affects a medical parameter (e.g., blood 5 pressure, blood cell count, cholesterol level) or disease state (e.g., cancer, autoimmune disorders, etc.). The terms "antagonist peptide" or "inhibitor peptide" refer to a peptide that blocks or in some way interferes with the biological activity of the associated protein of interest, or has biological activity comparable to a known antagonist or 10 inhibitor of the associated protein of interest. Thus, the term "Ang-2-antagonist peptide" comprises peptides that can be identified or derived as having Ang-2 antagonistic characteristics. Additionally, physiologically acceptable salts of the compounds of this invention are also encompassed herein. By "physiologically acceptable salts".is 15 meant any salts that are known or later discovered to be pharmaceutically acceptable: Some specific examples are: acetate; trifluoroacetate; hydrohalides, such as hydrochloride and hydrobromide; sulfate; citrate; tartrate; glycolate; and oxalate, mesylate, and phosphate. 20 Peptibodies One aspect of the present invention relates to development of Ang-2 peptibodies. The interaction of a protein ligand with its receptor often takes place at a relatively large interface. However, as demonstrated for human growth 25 hormone and its receptor, only a few key residues at the interface contribute to most of the binding energy. Clackson et al., Science 267: 383-6 (1995). The bulk of the protein ligand merely displays the binding epitopes in the right topology or serves functions unrelated to binding. Thus, molecules of only "peptide" length (generally 2 to 40 amino acids) can bind to the receptor protein of a given large 30 protein ligand. Such peptides may mimic the bioactivity of the large protein ligand ("peptide agonists") or, through competitive binding, inhibit the bioactivity of the large protein ligand ("peptide antagonists"). 41 - 42 Phage display technology has emerged as a powerful method in identifying such peptide agonists and antagonists. See, for example, Scott et al. Science 249: 386 (1990); Devlin et al., Science 249: 404 (1990); U.S. Patent No. 5,223,409, issued June 29, 1993; U.S. Patent No. 5,733,731, issued March 31, 1998; U.S. Patent No. 5,498,530, issued 5 March 12, 1996; U.S. Patent No. 5,432,018, issued July 11, 1995; U.S. Patent No. 5,338,665, issued August 16, 1994; U.S. Patent No. 5,922,545, issued July 13, 1999; WO 96/40987, published December 19, 1996; and WO 98/15833, published April 16, 1998. In peptide phage display libraries, random peptide sequences can be displayed by fusion with coat proteins of filamentous phage. The displayed peptides can be affinity 10 eluted against an antibody-immobilized extracellular domain of a receptor, if desired. The retained phage may be enriched by successive rounds of affinity purification and repropagation. The best binding peptides may be sequenced to identify key residues within one or more structurally related families of peptides. See, e.g., Cwirla et al., Science 276: 1696-9 (1997), in which two distinct families were identified. The peptide 15 sequences may also suggest which residues may be safely replaced by alanine scanning or by mutagenesis at the DNA level. Mutagenesis libraries may be created and screened to further optimize the sequence of the best binders. Lowman, Ann. Rev. Biophys. Biomol. Struct. 26: 401-24 (1997). Structural analysis of protein-protein interaction may also be used to suggest 20 peptides that mimic the binding activity of large protein ligands. In such an analysis, the crystal structure may suggest the identity and relative orientation of critical residues of the large protein ligand, from which a peptide may be designed. See, e.g., Takasaki et al., Nature Biotech 15: 1266-70 (1997). These analytical methods may also be used to investigate the interaction between a receptor protein and peptides selected by phage 25 display, which may suggest further modification of the peptides to increase binding affinity. Other methods compete with phage display in peptide research. A peptide library can be fused to the carboxyl terminus of the lac repressor and expressed in E. coli. Another E. coli-based method allows display on the cell's outer membrane by fusion with a peptidoglycan-associated lipoprotein (PAL). Hereinafter, these and related methods are collectively referred to as "E. coli display." In another methodtranslation-of random RNA is-halted prior to ribosome release, resulting in a library of polypeptides with their associated RNA still attached. Hereinafter, 5 this and related methods are collectively referred to as "ribosome display." Other methods employ chemical linkage of peptides to RNA. See, for example, Roberts and Szostak, Proc Natl Acad Sci USA, 94: 12297-303 (1997). Hereinafter, this and related methods are collectively referred to as "RNA-peptide screening." Chemically derived peptide libraries have been developed in which peptides are 10 immobilized on stable, non-biological materials, such as polyethylene rods or solvent-permeable resins. Another chemically derived peptide library uses photolithography to scan peptides immobilized on glass slides. Hereinafter, these and related methods are collectively referred to as "chemical-peptide screening." Chermical-peptide screening may be advantageous in that it allows use of D-amino 15 acids and other unnatural analogues, as well as non-peptide elements. Both biological and chemical methods are reviewed in Wells and Lowman, Curr. Opin. Biotechnol., 3: 355-62 (1992). Conceptually, one may discover peptide mimetics of any protein using phage display and the other methods mentioned above. These methods have been 20 used for epitope mapping, for identification of critical amino acids in protein protein interactions, and as leads for the discovery of new therapeutic agents. See, e.g., Cortese et al., Curr. Opin. Biotech. 7: 616-21 (1996). Peptide libraries are now being used most often in immunological studies, such as epitope mapping. See Kreeger, The Scientist 10(13):19-20(1996). 25 Peptides identified by phage display library screening have been regarded as "leads" in development of therapeutic agents rather than as therapeutic agents themselves. Like other proteins and peptides, they would likely be rapidly removed in vivo either by renal filtration, by cellular clearance mechanisms in the 30 reticuloendothelial system, or by proteolytic degradation [Francis, (supra)). As a result, the art presently uses peptides to validate drug targets or as scaffolds for 43 design-of organic compounds that might not have been as easily or as quickly identified through chemical library screening [Lowman, (supra); Kay et al., (supra)]. The art would benefit from a process by which such peptides could more readily yield therapeutic agents against angiogenesis. 5 Structure of Peptibodies In the compositions of matter prepared in accordance with this invention, the peptide may be attached to a vehicle through the peptide's N-terminus or C 10 terminus. Thus, vehicle-peptide molecules of this invention may be described by the following five formulae and multimers thereof: 44 (Xi)a-F-(X2)b (FORMULA I)

X

1 -Fi (FORMULA II)

F

1

-X

2 (FORMULA III) (FORMULA IV) F1-(L1)c-P-(L 2 )d-P2 (FORMULA V) wherein:

F

1 is a vehicle (preferably an Fc domain); 5 X 1 and X 2 are each independently selected from -(L 1 )c-P 1 , -(LI)c-P-(L 2 )d P 2 , -(L 1

)C-P

1

-(L

2 )d-P2-(L3)eP3, and -(LI)c-Pr(L2)d-P243)eP3-(L4)f-P4 Pi, P 2 , P 3 , and P 4 are each independently sequences of pharmacologically active peptides as described herein; 10 L 1 , L 2 , L 3 , and L 4 are each independently linkers; and "a", "b", "c", "d", "e", and "f' are each independently 0 or 1, provided that at least one of "a" and "b" is 1. Peptides 15 The present. invention contemplates peptides that selectively bind or specifically bind to Ang-2. Any number of such peptides may be used in conjunction with the present invention. Phage display, in particular, is useful in generating peptides for use in the present invention as has been shown that affinity selection from libraries of random peptides can be used to identify peptide ligands 20 for any site of any gene product. Dedman et al., J. Biol. Chem. 268: 23025-30 (1993). The peptides in this invention may be prepared by any of the methods disclosed in the art. Single letter amino acid abbreviations are used. The "X" in any sequence (and throughout this specification, unless specified otherwise in a 25 particular instance) means that any of the 20 naturally occurring amino acid residues, or any non-naturally occurring amino acids (described below under 45 -46 "Variants"), may be present. Any of these peptides may be linked in tandem (i.e., sequentially), with or without linkers, and tandem-linked examples are provided in the table. Linkers are listed as "L" and may be any of the linkers described herein. Tandem repeats and linkers are shown separated by dashes for clarity. Any peptide containing a 5 cysteinyl residue may be cross-linked with another Cys-containing peptide, either or both of which may be linked to a vehicle. Any peptide having more than one Cys residue may form an intrapeptide disulfide bond, as well. Any of these peptides may be derivatized as described herein. For derivatives in which the carboxyl terminus may be capped with an amino group, the capping amino group is -NH 2 . For derivatives in which 10 amino acid residues are substituted by moieties other than amino acid residues, the substitutions are denoted by S, which signifies any of the moieties described in Bhatnagar et al., J. Med. Chem. 39: 3814-9 (1996), and Cuthbertson et al., J Med. Chem. 40: 2876-82 (1997). All peptides are linked through peptide bonds unless otherwise noted. 15 Vehicles In one embodiment, this invention provides for at least one peptide to be attached to at least one vehicle (FI, F 2 ) through the N-terminus, C-terminus or a side chain of one of the amino acid residues of the peptide(s). Multiple vehicles may also be used; e.g., 20 Fc's at each terminus or an Fc at a terminus and a PEG group at the other terminus or a side chain. An Fc domain is one preferred vehicle. The Fc domain may be fused to the N or C termini of the peptides or at both the N and C termini. As noted above, Fc variants are suitable vehicles within the scope of this invention. 25 A native Fc may be extensively modified to form an Fc variant in accordance with this invention, provided binding to the salvage receptor is maintained. See, for example WO 97/34631 and WO 96/32478. In such Fc variants, one may remove one or more sites of a native Fc that provide structural features or functional activity not required by the fusion molecules of this invention. One may remove these sites by, for example, substituting or deleting residues, inserting residues into the site, or truncating portions containing the site. The inserted or substituted residues may also be altered amino acids, such as peptidomimetics or D-amino acids. Fc variants may be desirable for a number of 5 reasons, several of which are described below. Exemplary Fc variants include molecules and sequences in which: 1. Sites involved in disulfide bond formation are removed. Such removal may avoid reaction with other cysteine-containing proteins present in the host cell used to produce the molecules of the invention. For this purpose, the cysteine 10 containing segment at the N-terniinus may be truncated or cysteine residues may be deleted or substituted with other amino acids (e.g., alanyl, seryl). Even when cysteine residues are removed, the single chain Fc domains can still form a dimeric Fc domain that is held together non-covalently. 2. A native Fc is modified to make it more compatible with a selected host 15 cell. For example, one may remove the PA sequence near the N-terminus of a typical native Fc, which may be recognized by a digestive enzyme in E. coli such as proline iminopeptidase. One may also add an N-terminal methionyl residue, especially when the molecule is expressed recombinantly in a bacterial cell such as E. coli. 20 3. A portion of the N-terminus of a native Fc is removed to prevent N-terminal heterogeneity when expressed in a selected host cell. For this purpose, one may delete any of the first 20 amino acid residues at the N-terminus, particularly those at positions 1, 2, 3, 4 and 5. 4. One or more glycosylation sites are removed. Residues that are 25 typically glycosylated (e.g., asparagine) may confer cytolytic response. Such residues may be deleted or substituted with unglycosylated residues (e.g., alanine). 5. Sites involved in interaction with complement, such as the Clq binding site, are removed. For example, one may delete or substitute the EKK sequence 30 of human IgG1. Complement recruitment may not be advantageous for the molecules of this invention and so may be avoided with such an Fc variant. 47 - 48 6. Sites are removed that affect binding to Fc receptors other than a salvage receptor. A native Fc may have sites for interaction with certain white blood cells that are not required for the fusion molecules of the present invention and so may be removed. 5 7. The ADCC site is removed. ADCC sites are known in the art. See, for example, Molec. ImmunoL 29 (5):633-9 (1992) with regard to ADCC sites in IgGl. These sites, as well, are not required for the fusion molecules of the present invention and so may be removed. 8. When the native Fc is derived from a non-human antibody, the native Fc may 10 be humanized. Typically, to humanize a native Fc, one will substitute selected residues in the non-human native Fc with residues that are normally found in human native Fc. Techniques for antibody humanization are well known in the art. An alternative vehicle would be a protein, polypeptide, peptide, antibody, antibody fragment, or small molecule (e.g., a peptidomimetic compound) capable of binding to a 15 salvage receptor. For example, one could use as a vehicle a polypeptide as described in U.S. Patent No. 5,739,277, issued April 14, 1998 to Presta et al. Peptides could also be selected by phage display for binding to the FcRn salvage receptor. Such salvage receptor-binding compounds are also included within the meaning of "vehicle" and are within the scope of this invention. Such vehicles should be selected for increased half 20 life (e.g., by avoiding sequences recognized by proteases) and decreased immunogenicity (e.g., by favoring non-immunogenic sequences, as discovered in antibody humanization). As noted above, polymer vehicles may also be used for F, and F 2 . Various means for attaching chemical moieties useful as vehicles are currently available, see, e.g., 25 Patent Cooperation Treaty ("PCT") International Publication No. WO 96/11953, entitled "N-Terminally Chemically Modified Protein Compositions and Methods". This PCT publication discloses, among other things, the selective attachment of water soluble polymers to the N-terminus of proteins. A preferred polymer vehicle is polyethylene glycol (PEG). The PEG group may 30 be of any convenient molecular weight and may be linear or branched. The average molecular weight of the PEG will preferably range from about 2 kiloDalton ("kDa") to about 100 kDa, more preferably from about 5 kDa to about 50 kDa, most preferably from about 5 kDa to about 10 kDa. The PEG groups will generally be attached to the - 49 compounds of the invention via acylation or reductive alkylation through a reactive group on the PEG moiety (e.g., an aldehyde, amino, thiol, or ester group) to a reactive group on the inventive compound (e.g., an aldehyde, amino, or ester group). A useful strategy for the PEGylation of synthetic peptides consists of combining, 5 through forming a conjugate linkage in solution, a peptide and a PEG moiety, each bearing a special functionality that is mutually reactive toward the other. The peptides can be easily prepared with conventional solid phase synthesis as known in the art. The peptides are "preactivated" with an appropriate functional group at a specific site. The precursors are purified and fully characterized prior to reacting with the PEG moiety. 10 Ligation of the peptide with PEG usually takes place in aqueous phase and can be easily monitored by reverse phase analytical HPLC. The PEGylated peptides can be easily purified by preparative HPLC and characterized by analytical HPLC, amino acid analysis and laser desorption mass spectrometry. Polysaccharide polymers are another type of water soluble polymer which may be 15 used for protein modification. Dextrans are polysaccharide polymers comprised of individual subunits of glucose predominantly linked by cal-6 linkages. The dextran itself is available in many molecular weight ranges, and is readily available in molecular weights from about 1 kDa to about 70 kDa. Dextran is a suitable water-soluble polymer for use in the present invention as a vehicle by itself or in combination with another 20 vehicle (e.g., FEc). See, for example, WO 96/11953 and WO 96/05309. The use of dextran conjugated to therapeutic or diagnostic immunoglobulins has been reported; see, for example, European Patent Publication No. 0 315 456. Dextran of about 1 kDa to about 20 kDa is preferred when dextran is used as a vehicle in accordance with the present invention. 25 Linkers Any "linker" group is optional. When present, its chemical structure is not critical, since it serves primarily as a spacer. The linker is preferably made up of amino acids linked together by peptide bonds. Thus, in preferred embodiments, the linker is made up 30 of from I to 20 amino acids linked by peptide bonds, wherein the amino acids are selected from the 20 naturally occurring amino acids. One or more of these amino acids may be glycosylated, as is well understood by those in the art. In a more preferred embodiment, the I to 20 amino acids are selected from glycine, alanine, proline, - 50 asparagine, glutamine, and lysine. Even more preferably, a linker is made up of a majority of amino acids that are sterically unhindered, such as glycine and alanine. Thus, preferred linkers are polyglycines (particularly (Gly) 5 , (Gly) 8 ), poly(Gly-Ala), and polyalanines. Combinations of Gly and Ala are also preferred as is the linker 5 referred to herein as Ki and having an amino acid sequence set forth in the Examples herein. Non-peptide linkers are also possible. For example, alkyl linkers such as -NH

(CH

2 )s-C(O)-, wherein s = 2-20 can be used. These alkyl linkers may further be substituted by any non-sterically hindering group such as lower alkyl (e.g., CI-C 6 ) lower 10 acyl, halogen (e.g., Cl, Br), CN, NH 2 , phenyl, etc. An exemplary non-peptide linker is a PEG linker, and has a molecular weight of 100 to 5000 kDa, preferably 100 to 500 kDa. The peptide linkers may be altered to form derivatives in the same manner as described above. 15 Variants and Derivatives Variants and derivatives of the specific binding agents are included within the scope of the present invention. Included within variants are insertional, deletional, and substitutional variants. It is understood that a particular specific binding agent of the present invention may contain one, two or all three types of variants. Insertional and 20 substitutional variants may contain natural amino acids, unconventional amino acids (as set forth below), or both.

In one example, insertional variants are provided wherein one or more amino acid residues, either naturally occurring or unconventional amino acids, supplement a peptide or a peptibody amino acid sequence. Insertions may be located at either or both termini of the protein, or may be positioned within 5 internal regions of the peptibody amino acid sequence. Insertional variants with additional residues at either or both termini can include, for example, fusion proteins and proteins including amino acid tags or labels. Insertion variants include peptides and peptibodies wherein one or more amino acid residues are added to the peptide or peptibody amino acid sequence, or fragment thereof. 10 Variant products of the invention also include mature peptides and peptibodies wherein leader or signal sequences are removed, and the resulting proteins having additional amino terminal residues, which amino acids may be natural or non-natural. Specific binding agents (such as peptibodies) with an additional methionyl residue at amino acid position -1 (Met'-peptibody) are 15 contemplated, as are specific binding agents with additional methionine and lysine residues at positions -2 and -1 (Met-2-Lys--). Variants having additional Met, Met-Lys, Lys residues (or one or more basic residues, in general) are particularly useful for enhanced recombinant protein production in bacterial host cells. The invention also embraces specific binding agent variants having 20 additional amino acid residues that arise from use of specific expression systems. For example, use of commercially available vectors that express a desired polypeptide as part of glutathione-S-transferase (GST) fusion product provides the desired polypeptide having an additional. glycine residue at amino acid position -1 after cleavage of the GST component from the desired polypeptide. Variants 25 which result from expression in other vector systems are also contemplated, including those wherein poly-histidine tags are incorporated into the amino acid sequence, generally at the carboxy and/or amino terminus of the sequence. Insertional variants also include fusion proteins wherein the amino and/or carboxy termini of the peptide or peptibody is fused to another polypeptide, a 30 fragment thereof or amino acids which are not generally recognized to be part of any specific protein sequence. Examples of such fusion proteins are 51 immunogenic polypeptides, proteins with long circulating half lives, such as immunoglobulin constant regions, marker proteins, proteins or polypeptides that facilitate purification of the desired peptide or peptibody, and polypeptide sequences that promote formation of multimeric proteins (such as leucine zipper 5 motifs that are useful in dimer formation/stability). This type of insertional variant generally has all or a substantial portion of the native molecule, linked at the N- or C-terminus, to all or a portion of a second polypeptide. For example, fusion proteins typically employ leader sequences from other species to permit the recombinant expression of a protein in a 10 heterologous host. Another useful fusion protein includes the addition of an immunologically active domain, such as an antibody epitope, to facilitate purification of the fusion protein. Inclusion of a cleavage site at or near the fusion junction will facilitate removal of the extraneous polypeptide after purification. Other useful fusions include linking of functional domains, such as active sites 15 from enzymes, glycosylation domains, cellular targeting signals or transmembrane regions. There are various commercially available fusion protein expression systems that may be used in the present invention. Particularly useful systems include but are not limited to the glutathione-S-transferase (GST) system 20 (Pharmacia), the maltose binding protein system (NEB, Beverley, MA), the FLAG system (IB1, New Haven, CT), and the 6xHis system (Qiagen, Chatsworth, CA). These systems are capable of producing recombinant peptides and/or peptibodies bearing only a small number of additional amino acids, which are unlikely to significantly affect the activity of the peptide or peptibody. For 25 example, both the FLAG system and the 6xHis system add only short sequences, both of which are known to be poorly antigenic and which do not adversely affect folding of a polypeptide to its native conformation. Another N-terminal fusion that is contemplated to be useful is the fusion of a Met-Lys dipeptide at the N-terminal region of the protein or peptides. Such a fusion may produce 30 beneficial increases in protein expression or activity. 52 Other fusion systems produce polypeptide hybrids where it is desirable to excise the fusion partner from the desired peptide or peptibody. In one embodiment, the fusion partner is linked to the recombinant peptibody by a peptide sequence containing a specific recognition sequence for a protease. 5 Examples of suitable sequences are those recognized by the Tobacco Etch Virus protease (Life Technologies, Gaithersburg, MID) or Factor Xa (New England Biolabs, Beverley, MA). The invention also provides fusion polypeptides which comprises all or part of a peptibody or peptide of the present invention, in combination with 10 truncated tissue factor (tTF). tTF is a vascular targeting agent consisting of a truncated form of a human coagulation-inducing protein that acts as a tumor blood vessel clotting agent, as described U.S. Patent Nos.: 5,877,289; 6,004,555; 6,132,729; 6,132,730; 6,156,321; and European Patent No. EP 0988056. The fusion of tTF to the anti-Ang-2 peptibody or peptide, or fragments thereof 15 facilitates the delivery of anti-Ang-2 to target cells. In another aspect, the invention provides deletion variants wherein one or more amino acid residues in a peptide or peptibody are removed. Deletions can be effected at one or both termini of the peptibody, or from removal of one or more residues within the peptibody amino acid sequence. Deletion variants 20 necessarily include all fragments of a peptide or peptibody. In still another aspect, the invention provides substitution variants of peptides and peptibodies of the invention. Substitution variants include those peptides and peptibodies wherein one or more amino acid residues are removed and replaced with one or more alternative amino acids, which amino acids, may be 25 naturally occurring or non-naturally occurring. Substitutional variants generate peptides or peptibodies that are "similar" to the original peptide or peptibody, in that the two molecules have a certain percentage of amino acids that are identical. Substitution variants include substitutions of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, amino acids within a peptide or peptibody, wherein the number of 30 substitutions may be up to ten percent or more, of the amino acids of the peptide or peptibody. In one aspect, the substitutions are conservative in nature, however, 53 the invention embraces substitutions that are also non-conservative and also includes unconventional amino acids. Identity and similarity of related peptides and peptibodies can be readily calculated by known methods. Such methods include, but are not limited to, those 5 described in Computational Molecular Biology, Lesk, A.M., ed., Oxford University Press, New York (1988); Biocomputing: Informatics and Genome Projects, Smith, D.W., ed., Academic Press, New York (1993); Computer Analysis of Sequence Data, Part 1, Griffin, A.M., and Griffin, H.G., eds., Humana Press, New Jersey (1994); Sequence Analysis in Molecular Biology, von Heinje, 10 G., Academic Press (1987); Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M. Stockton Press, New York (1991); and Carillo et al., SIAM J. Applied Math., 48:1073 (1988). Preferred methods to determine the relatedness or percent identity of two peptides or polypeptides, or a polypeptide and a peptide, are designed to give the 15 largest match between the sequences tested. Methods to determine identity are described in publicly available computer programs. Preferred computer program methods to determine identity between two sequences include, but are not limited to, the GCG program package, including GAP (Devereux et al., Nucl. Acid. Res., 12:387 (1984); Genetics Computer Group, University of Wisconsin, Madison, 20 WI, BLASTP, BLASTN, and FASTA (Altschul et al., J. Mol. Biol., 215:403-410 (1990)). The BLASTX program is publicly available from the National Center for Biotechnology Information (NCBI) and other sources (BLAST Manual, Altschul et al. NCB/NLM/NIH Bethesda, MD 20894; Altschul et al., supra (1990)). The well-known Smith Waterman algorithm may also be used to determine identity. 25 Certain alignment schemes for aligning two amino acid sequences may result in the matching of only a short region of the two sequences, and this small aligned region may have very high sequence identity even though there is no significant relationship between the two full-length sequences. Accordingly, in certain embodiments, the selected alignment method (GAP program) will result in 30 an alignment that spans at least ten percent of the full length of the target 54 polypeptide being compared, i.e., at least 40 contiguous amino acids where sequences of at least 400 amino acids are being compared, 30 contiguous amino acids where sequences of at least 300 to about 400 amino acids are being compared, at least 20 contiguous amino acids where sequences of 200 to about 5 300 amino acids are being compared, and at least 10 contiguous amino acids where sequences of about 100 to 200 amino acids are being compared. For example, using the computer algorithm GAP (Genetics Computer Group, University of Wisconsin, Madison, WI), two polypeptides for which the percent sequence identity is to be determined are aligned for optimal matching of 10 their respective amino acids (the "matched span", as determined by the algorithm). In certain embodiments, a gap opening penalty (which is typically calculated as 3X the average diagonal; the "average diagonal" is the average of the diagonal of the comparison matrix being used; the "diagonal" is the score or number assigned to each perfect amino acid match by the particular comparison 15 matrix) and a gap extension penalty (which is usually 1/10 times the gap opening penalty), as well as a comparison matrix such as PAM 250 or BLOSUM 62 are used in conjunction with the algorithm. In certain embodiments, a standard comparison matrix (see Dayhoff et al., Atlas of Protein Sequence and Structure, 5(3)(1978) for the PAM 250 comparison matrix; Henikoff et al., Proc. Natl. Acad. 20 Sci USA, 89:10915-10919 (1992) for the BLOSUM 62 comparison matrix) is also used by the algorithm. In certain embodiments, the parameters for a polypeptide sequence comparison include the following: Algorithm: Needleman et al., J. Mol. Biol., 48:443-453 (1970); 25 Comparison matrix: BLOSUM 62 from Henikoff et al., supra (1992); Gap Penalty: 12 Gap Length Penalty: 4 Threshold of Similarity: 0 55 -56 The GAP program may be useful with the above parameters. In certain embodiments, the aforementioned parameters are the default parameters for polypeptide comparisons (along with no penalty for end gaps) using the GAP algorithm. In certain embodiments, the parameters for polynucleotide molecule sequence (as 5 opposed to an amino acid sequence) comparisons include the following: Algorithm: Needleman et al., supra (1970); Comparison matrix: matches = +10, mismatch = 0 Gap Penalty: 50 Gap Length Penalty: 3 10 The GAP program may also be useful with the above parameters. The aforementioned parameters are the default parameters for polynucleotide molecule comparisons. Other exemplary algorithms, gap opening penalties, gap extension penalties, comparison matrices, thresholds of similarity, etc. may be used, including those set forth 15 in the Program Manual, Wisconsin Package, Version 9, September, 1997. The particular choices to be made will be apparent to those of skill in the art and will depend on the specific comparison to be made, such as DNA-to-DNA, protein-to-protein, protein-to DNA; and additionally, whether the comparison is between given pairs of sequences (in which case GAP or BestFit are generally preferred) or between one sequence and a large 20 database of sequences (in which case FASTA or BLASTA are preferred). As used herein, the twenty conventional amino acids and their abbreviations follow conventional usage. See Immunology--A Synthesis (2nd Edition, E. S. Golub and D. R. Gren, Eds., Sinauer Associates, Sunderland, Mass. (1991)).

The amino acids may have either L or D stereochemistiy (except for Gly, which is neither L nor D) and the polypeptides and compositions of the present invention may comprise a combination of stereochemistries. However, the L stereochemistry is preferred. The invention also provides reverse molecules 5 wherein the amino terminal to carboxy terminal sequence of the amino acids is reversed. For example, the reverse of a molecule having the normal sequence X 1 X 2

-X

3 would be X 3

-X

2

-X

1 . The invention also provides retro-reverse molecules wherein, as above, the amino terminal to carboxy terminal sequence of amino acids is reversed and residues that are normally "L" enantiomers are altered to the 10 "D" stereoisomer form. Stereoisomers (e.g., D-amino acids) of the twenty conventional amino acids, unnatural amino acids such as o-, a-disubstituted amino acids, N-alkyl amino acids, lactic acid, and other unconventional amino acids may also be suitable components for polypeptides of the present invention. Examples of 15 unconventional amino acids include, without limitation: aminoadipic acid, beta alanine, beta-aminopropionic acid, aminobutyric acid, piperidinic acid, aminocaprioic acid, aminoheptanoic acid, aminoisobutyric acid, aminopimelic acid, diaminobutyric acid, desmosine, dianinopimelic acid, diaminopropionic acid, N-ethylglycine, N-ethylaspargine, hyroxylysine, allo-hydroxylysine, 20 hydroxyproline, isodesmosine, allo-isoleucine, N-methylglycine, sarcosine, N-methylisoleucine, N-methylvaline, norvaline, norleucine, orithine, 4 hydroxyproline, y-carboxyglutamate, E-N,N,N-trimethyllysine, E-N-acetyllysine, O-phosphoserine, N-acetylserine, N-formylmethionine, 3-methylhistidine, 5 hydroxylysine, o-N-methylarginine, and other similar amino acids and amino 25 acids (e.g., 4-hydroxyproline). Similarly, unless specified otherwise, the left-hand end of single-stranded polynucleotide sequences is the 5' end; the left-hand direction of double-stranded polynucleotide sequences is referred to as the 5' direction. The direction of 5' to 3' addition of nascent RNA transcripts is referred to as the transcription direction; 30 sequence regions on the DNA strand having the same sequence as the RNA. and 57 which are 5' to the 5'end of the RNA transcript are referred to as "upstream sequences"; sequence regions on the DNA strand having the same sequence as the RNA and which are 3' to the 3' end of the RNA transcript are referred to as "downstream sequences". 5 It will be appreciated that amino acid residues can be divided into classes based on their common side chain properties: 1. Neutral Hydrophobic: Alanine (Ala; A), Valine (Val; V), Leucine (Leu; L), Isoleucine (Ile; I), Proline (Pro; P), Tryptophan (Trp; W), Phenylalanine (Phe; F), and Methionine (Met, M). 10 2. Neutral Polar: Glycine (Gly; G); Serine (Ser; S), Threonine (Thr; T), Tyrosine (Tyr; Y), Cysteine (Cys; C), Glutamine (Glu; Q), Asparagine (Asn; N), and Norleucine. 3. Acidic: Aspartic Acid (Asp; D), Glutamic Acid (Glu; E); 4) Basic: Lysine (Lys; K), Arginine (Arg; R), Histidine (His; H). 15 See Lewin, B., Genes V, Oxford University Press (1994), p.11. Conservative amino acid substitutions may encompass unconventional amino acid residues, which are typically incorporated by chemical peptide synthesis rather than by synthesis in biological systems. These include, without limitation, peptidomimetics and other reversed or inverted forms of amino acid 20 moieties. Non-conservative substitutions may involve the exchange of a member of one of these classes for a member from another class. In making such changes, according to certain embodiments, the hydropathic index of amino. acids may be considered. Each amino acid has been assigned a hydropathic index on the basis of its hydrophobicity and charge 25 characteristics. They are: isoleucine (+4.5); valine (+4.2); leucine (+3.8); phenylalanine (+2.8); cysteine/cystine (+2.5); methionine (+1.9); alanine (+1.8); glycine (-0.4); threonine (-0.7); serine (-0.8); tryptophan (-0.9); tyrosine (-1.3); proline (-1.6); histidine (-3.2); glutamate (-3.5); glutamine (-3.5); aspartate (-3.5); asparagine (-3.5); lysine (-3.9); and arginine (-4.5). 58 The importance of the hydropathic amino acid index in conferring interactive biological function on a protein is understood in the art. Kyte et al., J. Mol. Biol., 157:105-131 (1982). It is known that-certain amino acids may be substituted for other amino acids having a similar hydropathic index or score and 5 still retain a similar biological activity. In making changes based upon the hydropathic index, in certain embodiments, the substitution of amino acids whose hydropathic indices are within ±2 is included. In certain embodiments, those which are within ±1 are included, and in certain embodiments, those within ±0.5 X are included. 10 It is also understood in the art that the substitution of like amino acids can be made effectively on the basis of hydrophilicity, particularly where the biologically functional peptibody or peptide thereby created is intended for use in immunological embodiments, as in the present case. In certain embodiments, the greatest local average hydrophilicity of a protein, as governed by the 15 hydrophilicity of its adjacent amino acids, correlates with its immunogenicity and antigenicity, i.e., with a biological property of the protein. The following hydrophilicity values have been assigned to these amino acid residues: arginine (+3.0); lysine (+3.0); aspartate (+3.0 ± 1); glutamate (+3.0 ± 1); serine (+0.3); asparagine (+0.2); glutamine (+0.2); glycine (0); threonine ( 20 0.4); proline (-0.5 ± 1); alanine (-0.5); histidine (-0.5); cysteine (-1.0); methionine (-1.3); valine (-1.5); leucine (-1.8); isoleucine (-1.8); tyrosine (-2.3); phenylalanine (-2.5) and tryptophan (-3.4). In making changes based upon similar hydrophilicity values, in certain embodiments, the substitution of amino acids whose hydrophilicity values are within ±2 is included, in certain embodiments, 25 those which are within ±1 are included, and in certain embodiments, those within ±0.5 are included. One may also identify epitopes from primary amino acid sequences on the basis of hydrophilicity. These regions are also referred to as "epitopic core regions." Exemplary amino acid substitutions are set forth in Table 2 below. 59 Table 2 Amino Acid Substitutions Original Residues Exemplary Substitutions Preferred Substitutions Ala Val, Leu, Ile Val Arg Lys, Gln, Asn Lys Asn Gln, Glu, Asp Gln Asp Glu, Gln, Asp Glu Cys Ser, Ala Ser Gln Asn, Glu, Asp Asn Glu Asp, Gin, Asn Asp Gly Pro, Ala Ala His Asn, Gln, Lys, Arg Arg Ile Leu, Val, Met, Ala, Phe, Leu Norleucine Leu Norleucine, Be, Val, Met, fle Ala, Phe Lys Arg, 1,4 Diamino-butyric Arg Acid, Gln, Asn Met Leu, Phe, fle Leu Phe Leu, Val, Ile, Ala, Tyr Leu Pro Ala Gly Ser Thr, Ala, Cys Thr Thr Ser Ser Trp Tyr, Phe Tyr Tyr Trp, Phe, Thr, Ser Phe Val Ile, Met, Leu, Phe, Ala, Leu Norleucine A skilled artisan will be able to determine suitable variants of the 5 polypeptide as set forth herein using well-known techniques. In certain embodiments, one skilled in the art may identify suitable areas of the molecule that may be changed without destroying activity by targeting regions not believed 60 to be important for activity. In certain embodiments, one can identify residues and portions of the molecules that are conserved among similar peptides or polypeptides. In certain embodiments, even areas that may be important for biological activity or for structure may be subject to conservative amino acid 5 substitutions without destroying the biological activity or without adversely affecting the polypeptide structure. Additionally, one skilled in the art can review structure-function studies identifying residues in similar polypeptides that are important for activity or structure. In view of such a comparison, one can predict the importance of amino 10 acid residues in a protein that correspond to amino acid residues which are important for activity or structure in similar proteins. One skilled in the art may opt for chemically similar amino acid substitutions for such predicted important amino acid residues. One skilled in the art can also analyze the three-dimensional structure and 15 amino acid sequence in relation to that structure in similar polypeptides. In view of such information, one skilled in the art may predict the alignment of amino acid residues of an antibody with respect to its three dimensional structure. In certain embodiments, one skilled in the art may choose not to make radical changes to amino acid residues predicted to be on the surface of the protein, since such 20 residues may be involved in important interactions with other molecules. Moreover, one skilled in the art may generate test variants containing a single amino acid substitution at each desired amino acid residue. The variants can then be screened using activity assays known to those skilled in the art. Such variants could be used to gather information about suitable variants. For example, if one 25 discovered that a change to a particular amino acid residue resulted in destroyed, undesirably reduced, or unsuitable activity, variants with such a change may be avoided. In other words, based on information gathered from such routine experiments, one skilled in the art can readily determine the amino acids where further substitutions should be avoided either alone or in combination with other 30 mutations. 61 A number of scientific publications have been devoted to the prediction of secondary structure. See Moult J., Curr. Op. in Biotech., 7(4):422-427 (1996), Chou et al., Biochemistry, 13(2):222-245 (1974); Chou et al., Biochemistry, 113(2):211-222 (1974); Chou et al., Adv. Enzymol. Relat. Areas Mol. Biol., 47:45 5 148 (1978); Chou et al., Ann. Rev. Biochem., 47:251-276 and Chou et al., Biophys. J., 26:367-384 (1979). Moreover, computer programs are currently available to assist with predicting secondary structure. One method of predicting secondary structure is based upon homology modeling. For example, two polypeptides or proteins which have a sequence identity of greater than 30%, or 10 similarity greater than 40% often have similar structural topologies. The recent growth of the protein structural database (PDB) has provided enhanced predictability of secondary structure, including the potential number of folds within a polypeptide's or protein's structure. See Holm et al., Nucl. Acid. Res., 27(1):244-247 (1999). It has been suggested (Brenner et al., Curr. Op. Struct. 15 Biol., 7(3):369-376 (1997)) that there are a limited number of folds in a given polypeptide or protein and that once a critical number of structures have been resolved, structural prediction will become dramatically more accurate. Additional methods of predicting secondary structure include "threading" (Jones, D., Curr. Opin. Struct. Biol., 7(3):377-87 (1997); Sippl et al., Structure, 20 4(1):15-19 (1996)), "profile analysis" (Bowie et al., Science, 253:164-170 (1991); Gribskov et al., Meth. Enzym., 183:146-159 (1990); Gribskov et al., Proc. Nat. Acad. Sci., 84(13):4355-4358 (1987)), and "evolutionary linkage" (See Holm, supra (1999), and Brenner, supra (1997)). In certain embodiments, peptibody variants include glycosylation variants 25 wherein one or more glycosylation sites, such as a N-linked glycosylation site, has been added to the peptibody. An N-linked glycosylation site is characterized by the sequence: Asn-X-Ser or Asn-X-Thr, wherein the amino acid residue designated as X may be any amino acid residue except proline. The substitution or addition of amino acid residues to create this sequence provides a potential new 30 site for the addition of an N-linked carbohydrate chain. Alternatively, 62 substitutions which eliminate this sequence will remove an existing N-linked carbohydrate chain. Also provided is a rearrangement of N-linked carbohydrate chains wherein one or more N-linked glycosylation sites (typically those that are naturally occurring) are eliminated and one or more new N-linked sites are 5 created. The invention also provides "derivatives" that include peptibodies bearing modifications other than, or in addition to, insertions, deletions, or substitutions of amino acid residues. Preferably, the modifications are covalent in nature, and include for example, chemical bonding with polymers, lipids, other organic, and 10 inorganic moieties. Derivatives of the invention may be prepared to increase circulating half-life of a peptibody, or may be designed to improve targeting capacity for the peptibody to desired cells, tissues, or organs. Exemplary derivatives include moieties wherein one or more of the following modifications have been made: 15 e One or more of the peptidyl [-C(O)NR-] linkages (bonds) have been replaced by a non-peptidyl linkage such as a -CH 2 -carbamate linkage [-CH 2 -OC(O)NR-]; a phosphonate linkage; a -CH 2 sulfonamide [-CH 2 -S(0) 2 NR-] linkage; a urea [-NHC(O)NH-] linkage; a -CH 2 -secondary amine linkage; or an alkylated peptidyl 20 linkage [-C(O)NR 6 - where R 6 is lower alkyl]; e Peptides wherein the N-terminus is derivatized to a -NRR 1 group; to a -NRC(O)R group; to a -NRC(O)OR group; to a -NRS(O) 2 R group; to a -NHC(O)NBR group, where R and R 1 are hydrogen or lower alkyl, with the proviso that R and R 1 are not both hydrogen; 25 to a succinimide group; to a benzyloxycarbonyl-NH- (CBZ-NH-) group; or to a benzyloxycarbonyl-NH- group having from 1 to 3 substituents on the phenyl ring selected from the group consisting of lower alkyl, lower alkoxy, chloro, and bromo; and * Peptides wherein the free C terminus is derivatized to -C(O)R 2 30 where R 2 is selected from the group consisting of lower alkoxy and 63

-NR

3

R

4 where R 3 and R 4 are independently selected from the group consisting of hydrogen and lower alkyl. By "lower" is meant a group having from 1 to 6 carbon atoms. Additionally, modifications of individual amino acids may be introduced 5 into the polypeptides or compositions of the invention by reacting targeted amino acid residues of the peptide with an organic derivatizing agent that is capable of reacting with selected side chains or terminal residues. The following are exemplary: Lysinyl and amino terminal residues may be reacted with succinic or other 10 carboxylic acid anhydrides. Derivatization with these agents has the effect of reversing the charge of the lysinyl residues. Other suitable reagents for derivatizing alpha-amino-containing residues include imidoesters such as methyl picolinimidate; pyridoxal phosphate; pyridoxal; chloroborohydride; trinitrobenzenesulfonic acid; O-methylisourea; 2,4 pentanedione; and 15 transaminase-catalyzed reaction with glyoxylate. Arginyl residues may be modified by reaction with one or several conventional reagents, among them phenylglyoxal, 2,3-butanedione, 1,2 cyclohexanedione, and ninhydrin. Derivatization of arginine residues requires that the reaction be performed in alkaline conditions because of the high pKa of 20 the guanidine functional group. Furthermore, these reagents may react with the groups of lysine as well as the arginine guanidino group. The specific modification of tyrosyl residues per se has been studied extensively, with particular interest in introducing spectral labels into tyrosyl residues by reaction with aromatic diazonium compounds or tetranitromethane. 25 Most commonly, N-acetylimidizole and tetranitromethane may be used to form 0 acetyl tyrosyl species and 3-nitro derivatives, respectively. Carboxyl side groups (aspartyl or glutamyl) may be selectively modified by reaction with carbodiimides (R'-N=C=N-R') such as 1-cyclohexyl-3-(2 morpholinyl-(4-ethyl) carbodiimide or 1-ethyl-3-(4-azonia-4,4-dimethylpentyl) 30 carbodiimide. Furthermore, aspartyl and glutamyl residues may be converted to asparaginyl and glutaminyl residues by reaction with ammonium ions. 64 Glutaminyl and asparaginyl residues are frequently deamidated to the corresponding glutamyl and aspartyl residues. Alternatively, these residues may be deamidated under mildly acidic conditions. Either form of these residues falls within the scope of this invention. 5 Derivatization with bifunctional agents is useful for cross-linking the peptides or their functional derivatives to a water-insoluble support matrix or to other macromolecular carriers. Commonly used cross-linking agents include, e.g., 1,1-bis(diazoacetyl)-2-phenylethane, glutaraldehyde, N-hydroxysuccinimide esters, for example, esters with 4-azidosalicylic acid, homobifunctional 10 imidoesters, including disuccinimidyl esters such as 3,3'-dithiobis (succinimidylpropionate), and bifunctional maleimides such as bis-N-maleimido 1,8-octane. Derivatizing agents such as methyl-3-[(p azidophenyl)dithio]propioimidate yield photoactivatable intermediates that are capable of forming crosslinks in the presence of light. Alternatively, reactive 15 water-insoluble matrices such as cyanogen bromide-activated carbohydrates and the reactive substrates described in U.S. Pat. Nos. 3,969,287; 3,691,016; 4,195,128; 4,247,642; 4,229,537; and 4,330,440 may be employed for protein immobilization. Other possible modifications include hydroxylation of proline and lysine, 20 phosphorylation of hydroxyl groups of seryl or threonyl residues, oxidation of the sulfur atom in Cys, methylation of the alpha-amino groups of lysine, arginine, and histidine side chains (Creighton, T.E., Proteins: Structure and Molecule Properties, W. H. Freeman &-Co., San Francisco, pp. 79-86 (1983)), acetylation of the N-terminal amine, and, in some instances, amidation of the C-terminal 25 carboxyl groups. Such derivatized moieties preferably improve one or more characteristics including anti-angiogenic activity, solubility, absorption, biological half life, and the like of the compounds. Alternatively, derivatized moieties may result in compounds that have the same, or essentially the same, characteristics and/or 30 properties of the compound that is not derivatized. The moieties may alternatively eliminate or attenuate any undesirable side effect of the compounds and the like. 65 - 66 Compounds of the present invention may be changed at the DNA level, as well. The DNA sequence of any portion of the compound may be changed to codons more compatible with the chosen host cell. For E coli, which is the preferred host cell, optimized codons are known in the art. Codons may be substituted to eliminate restriction sites or to include silent 5 restriction sites, which may aid in processing of the DNA in the selected host cell. The vehicle, linker and peptide DNA sequences may be modified to include any of the foregoing sequence changes. Thus, all modifications, substitution, derivitizations, etc. discussed herein apply equally to all aspects of the present invention, including but not limited to peptides, peptide dimers and multimers, linkers, and vehicles. 10 Additionally, one skilled in the art can review structure-function studies identifying residues in similar peptides that are important for activity or structure. In view of such a comparison, one can predict the importance of amino acid residues in a peptide that correspond to amino acid residues that are important for activity or structure in similar peptides. One skilled in the art may opt for chemically similar amino acid substitutions for 15 such predicted important amino acid residues of the peptides. One skilled in the art can also analyze the three-dimensional structure and amino acid sequence in relation to that structure in similar polypeptides. In view of that information, one skilled in the art may predict the alignment of amino acid residues of a peptide with respect to its three dimensional structure. One skilled in the art may choose not to make 20 radical changes to amino acid residues predicted to be on the surface of the protein, since such residues may be involved in important interactions with other molecules. Moreover, one skilled in the art may generate test variants containing a single amino acid substitution at each desired amino acid residue. The variants can then be screened using activity assays known to those skilled in the art. Such data could be used to gather information about 25 suitable variants. For example, if one discovered that a change to a particular amino acid residue resulted in destroyed, undesirably reduced, or unsuitable activity, variants with such a change would be avoided. In other words, based on information gathered from such routine experiments, one skilled in the art can readily determine the amino acids where further substitutions should be avoided either alone or in combination with other mutations. A number of scientific publications have been devoted to the prediction of secondary structure. See Moult J., Curr. Op. in Biotech., 7(4): 422-427 (1996), 5 Chou et al., Biochemistry, 13(2): 222-245 (1974); Chou et al., Biochemistry, 113(2): 211-222 (1974); Chou et al., Adv. Enzymol. Relat. Areas Mol. Biol., 47: 45-148 (1978); Chou et aL, Ann. Rev. Biochem., 47: 251-276 and Chou et al., Biophys. J., 26: 367-384 (1979). Moreover, computer programs are currently available to assist with predicting secondary structure. One method of predicting 10 secondary structure is based upon homology modeling. For example, two polypeptides or proteins which have a sequence identity of greater than 30%, or similarity greater than 40% often have similar structural topologies. The recent growth of the protein structural data base (PDB) has provided enhanced predictability of secondary structure, including the potential number of folds 15 within a polypeptide's or protein's structure. See Holm et al., Nucl. Acid. Res., 27(1): 244-247 (1999). It has been suggested (Brenner et al., Curr. Op. Struct. Biol., 7(3): 369-376 (1997)) that there are a limited number of folds in a given polypeptide or-protein and that once a critical number of structures have been resolved, structural prediction will gain dramatically in accuracy. 20 Additional methods of predicting secondary structure include "threading" (Jones, D., CuT. Opin. Struct. Biol., 7(3): 377-87 (1997); Sippl et al., Structure, 4(1): 15-9 (1996)), "profile analysis" (Bowie et al., Science, 253: 164-170 (1991); Gribskov et al., Meth. Enzym., 183: 146-159 (1990); Gribskov et al., Proc. Nat. Acad. Sci., 84(13): 4355-8 (1987)), and "evolutionary linkage" (See Home, supra, 25 and Brenner, supra). The invention further embraces derivative specific binding agents, e.g. peptibodies, covalently modified to include one or more water soluble polymer attachments, such as polyethylene glycol, polyoxyethylene glycol, or polypropylene glycol, as described U.S. Patent Nos.: 4,640,835; 4,496,689; 30 4,301,144; 4,670,417; 4,791,192; and 4,179,337. Still other useful polymers known in the art include monomethoxy-polyethylene glycol, dextran, cellulose, or 67 other carbohydrate based polymers, poly-(N-vinyl pyrrolidone)-polyethylene glycol, propylene glycol homopolymers, a polypropylene oxide/ethylene oxide co-polymer, polyoxyethylated polyols (e.g., glycerol) and polyvinyl alcohol, as well as mixtures of these polymers. Particularly preferred are peptibodies 5 covalently modified with polyethylene glycol (PEG) subunits. Water-soluble polymers may be bonded at specific positions, for example at the amino terminus of the peptibodies, or randomly attached to one or more side chains of the polypeptide. The use of PEG for improving the therapeutic capacity for specific binding agents-, e.g. peptibodies, and for humanized antibodies in particular, is 10 described in US Patent No. 6, 133, 426 to Gonzales et al., issued October 17, 2000. The invention also contemplates derivatizing the peptide and/or vehicle portion of the compounds. Such derivatives may improve the solubility, absorption, biological half-life, and the like of the compounds. The moieties may 15 alternatively eliminate or attenuate any undesirable side-effect of the compounds and the like. Exemplary derivatives include compounds in which: 1. The compound or some portion thereof is cyclic. For example, the peptide portion may be modified to contain two or more Cys residues (e.g., in the linker), which could cyclize by disulfide bond formation. 20 2. The compound is cross-linked or is rendered capable of cross-linking between molecules. For example, the peptide portion may be modified to contain one Cys residue and thereby be able to form an intermolecular disulfide bond with a like molecule. The compound may also be cross-linked through its C-terminus. 3. One or more peptidyl [-C(O)NR-] linkages (bonds).is replaced by a 25 non-peptidyl linkage. Exemplary non-peptidyl linkages are -CH 2 -carbamate [

CH

2 -OC(O)NR-], phosphonate, -CIH2-sulfonamide [-CH 2

-S(O)

2 NR-], urea [ NHC(O)NH-], -CH 2 -secondary amine, and alkylated peptide [-C(O)NR 6 - wherein

R

6 is lower alkyl]. 4. The N-terminus is derivatized. Typically, the N-terminus may be 30 acylated or modified to. a substituted amine. Exemplary N-terminal derivative groups include -NRR 1 (other than -NH 2 ), -NRC(O)R 1 , -NRC(O)OR 1 , 68

NRS(O)

2

R

1 , -NHC(O)NHR 1 , succinimide, or benzyloxycarbonyl-NH- (CBZ NH-), wherein R and-RI are each independently hydrogen or lower alkyl and wherein the phenyl ring may be substituted with 1 to 3 substituents selected from the group consisting of C-C 4 alkyl, C-C 4 alkoxy, chloro, and bromo. 5 5. The free C-terminus is derivatized. Typically, the C-terminus is esterified or amidated. For example, one may use methods described in the art to add (NH-CH 2

-CH

2

-NH

2

)

2 to compounds of this invention at the C-terminus. Likewise, one may use methods described in the art to add -NH 2 to compounds of this invention at the C-terminus. Exemplary C-terminal derivative groups include, 10 for example, -C(O)R 2 wherein R 2 is lower alkoxy or -NR 3

R

4 wherein R 3 and R 4 are independently hydrogen or C-Cs alkyl (preferably C-C 4 alkyl). 6. A disulfide bond is replaced with another, preferably more stable, cross-linking moiety (e.g., an alkylene). See, e.g., Bhatnagar (supra); Alberts et al., Thirteenth Am. Pep. Symp., 357-9 (1993). 15 7. One or more individual amino acid residues is modified. Various derivatizing agents are known to react specifically with selected side chains or terminal residues, as described in detail below. Lysinyl residues and amino terminal residues may be reacted with succinic or other carboxylic acid anhydrides, which reverse the charge of the lysinyl 20 residues. Other suitable reagents for derivatizing alpha-amino-containing residues include imidoesters such as methyl picolinimidate; pyridoxal phosphate; pyridoxal; chloroborohydride; trinitrobenzenesulfonic acid; O-methylisourea; 2,4 pentanedione; and transaminase-catalyzed reaction with glyoxylate. Arginyl residues may be modified by reaction with any one or 25 combination of several conventional reagents, including phenylglyoxal, 2,3 butanedione, 1,2-cyclohexanedione, and ninhydrin. Derivatization of arginyl residues requires that the reaction be performed in alkaline conditions because of the high pKa of the guanidine functional group. Furthermore, these reagents may react with the groups of lysine as well as the arginine epsilon-amino group. 30 Specific modification of tyrosyl residues has been studied extensively, with particular interest in introducing spectral labels into tyrosyl residues by 69 reaction with aromatic diazonium compounds or tetranitromethane. Most commonly, N-acetylimidizole and tetranitromethane are used to form O-acetyl tyrosyl species and 3-nitro derivatives, respectively. Carboxyl side chain groups (aspartyl or glutamyl) may be selectively 5 modified by reaction with carbodiimides (R'-N=C=N-R') such as 1-cyclohexyl-3

(

2 -morpholinyl-(4-ethyl) carbodiimide or 1-ethyl-3-( 4 -azonia-4,4-dimethylpentyl) carbodiimide. Furthermore, aspartyl and glutamyl residues may be converted to asparaginyl and glutaminyl residues by reaction with. ammonium ions. Glutaminyl and asparaginyl residues-may be deamidated to the 10 corresponding glutamyl and aspartyl residues. Alternatively, these residues are deamidated under mildly acidic conditions. Either form of these residues falls within the scope of this invention. Cysteinyl residues can be replaced by amino acid residues or other moieties either to eliminate disulfide bonding or, conversely, to stabilize cross 15 linking. See, e.g., Bhatnagar, (supra). Derivatization with bifunctional agents is useful for cross-linking the peptides or their functional derivatives to a water-insoluble support matrix or to other macromolecular vehicles. Commonly used cross-linking agents include, e.g., 1,1-bis(diazoacetyl)-2-phenylethane, glutaraldehyde, N-hydroxysuccinimide 20 esters, for example, esters with 4-azidosalicylic acid, homobifunctional imidoesters, including disuccinimidyl esters such as 3,3' dithiobis(succinimidylpropionate), and bifunctional maleimides such as bis-N maleimido-1,8-octane. Derivatizing agents such as methyl-3-[(p azidophenyl)dithio]propioimidate yield photoactivatable intermediates that are 25 capable of forming crosslinks in the presence of light. Alternatively, reactive water-insoluble matrices such as cyanogen bromide-activated carbohydrates and the reactive substrates described in U.S. Patent Nos. 3,969,287; 3,691,016; 4,195,128; 4,247,642; 4,229,537; and 4,330,440 are employed for protein immobilization. 30 Carbohydrate (oligosaccharide) groups may conveniently be attached to sites that are known to be glycosylation sites in proteins. Generally, 0-linked 70 oligosaccharides are attached to serine-(Ser) or threonine (Thr) residues while N linked oligosaccharides are attached to asparagine (Asn) residues when they are part of the sequence Asn-X-Ser/Thr, where X can be any amino acid except proline. X is preferably one of the 19 naturally occurring amino acids other than 5 proline. The structures of N-linked and 0-linked oligosaccharides and the sugar residues found in each type are different. One type of sugar that is commonly found on both is N-acetylneuraminic acid (referred to as sialic acid). Sialic acid is usually the terminal residue of both N-linked and 0-linked oligosaccharides and, by virtue of its negative charge, may confer acidic properties to the glycosylated 10 compound. Such site(s) may be incorporated in the linker of the compounds of this invention and are preferably glycosylated by a cell during recombinant production of the polypeptide compounds (e.g., in mammalian cells such as CHO, BHK, COS). However, such sites may further be glycosylated by synthetic or. semi-synthetic procedures known in the art. 15 Other possible modifications include hydroxylation of proline and lysine, phosphorylation of hydroxyl groups of seryl or threonyl residues, oxidation of the sulfur atom in Cys, methylation of the alpha-amino. groups of lysine, arginine, and histidine side chains [Creighton, Proteins: Structure and Molecule Properties (W. H. Freeman & Co., San Francisco), pp. 79-86 (1983)]. 20 Compounds of the present invention may be changed at the DNA level, as well. The DNA sequence of any portion of the compound may be changed to codons more compatible with the chosen host cell. For E. coli, which is the preferred host cell, optimized codons are known in the art. Codons may be substtuted to eliminate restriction sites or to include silent restriction sites, which 25 may aid in processing of the DNA in the selected host cell. The vehicle, linker and peptide DNA sequences may be modified to include any of the foregoing sequence changes. 30 Affinity Maturation One embodiment of the-present invention includes "affinity matured" peptides and peptibodies. This procedure contemplates increasing the affinity oi 71 the bio-activity of the peptides and peptibodies of the present invention using phage display or other selection technologies. Based on a consensus sequence (which is generated for a collection of related peptides), directed secondary phage display libraries can be generated in which the "core" amino acids (determined 5 from the consensus sequence) are held constant or are biased in frequency of occurrence. Alternatively, an individual peptide sequence can be used to generate a biased, directed phage display library. Panning of such libraries can yield peptides (which can be converted to peptibodies) with enhanced binding to Ang-2 or with enhanced bio-activity. 10 Non-Peptide Analogs/Protein Mimetics Furthermore, non-peptide analogs of peptides that provide a stabilized structure or lessened biodegradation, are also contemplated. Peptide mimetic 15 analogs can be prepared based on a selected inhibitory peptide by replacement of one or more residues by nonpeptide moieties. Preferably, the nonpeptide moieties permit the peptide to retain its natural confirmation, or stabilize a preferred, e.g., bioactive, confirmation which retains the ability to recognize and bind Ang-2. In one aspect, the resulting analog/mimetic exhibits increased binding affinity for 20 Ang-2. One example of methods for preparation of nonpeptide mimetic analogs from peptides is described in Nachman et al., Regul. Pept. 57:359-370 (1995). If desired, the peptides of the invention can be modified, for instance, by glycosylation, amidation, carboxylation, or phosphorylation, or by the creation of acid addition salts, amides, esters, in particular C-terminal esters, and N-acyl 25 derivatives of the peptides of the invention. The peptibodies also can be modified to create peptide derivatives by forming covalent or noncovalent complexes with other moieties. Covalently-bound complexes can be prepared by linking the chemical moieties to functional groups on the side chains of amino acids comprising the peptibodies, or at the N- or C-terminus. 30 In particular, it is anticipated that the peptides can be conjugated to a reporter group, including, but not limited to a radiolabel, a fluorescent label, an enzyme (e.g., that catalyzes a colorimetric or fluorometric reaction), a substrate, a 72 - 73 solid matrix, or a carrier (e.g., biotin or avidin). The invention accordingly provides a molecule comprising a peptibody molecule, wherein the molecule preferably further comprises a reporter group selected from the group consisting of a radiolabel, a fluorescent label, an enzyme, a substrate, a solid matrix, and a carrier. Such labels are 5 well known to those of skill in the art, e.g., biotin labels are particularly contemplated. The use of such labels is well known to those of skill in the art and is described in, e.g., U.S. Patent Nos.3,817,837; 3,850,752; 3,996,345; and 4,277,437. Other labels that will be useful include but are not limited to radioactive labels, fluorescent labels and chemiluminescent labels. U.S. Patents concerning use of such labels include, for 10 example, U.S. Patent Nos. 3,817,837; 3,850,752; 3,939,350; and 3,996,345. Any of the peptibodies of the present invention may comprise one, two, or more of any of these labels. 15 Methods of Making Peptides The peptides of the present invention can be generated using a wide variety of techniques known in the art. For example, such peptides can be synthesized in solution or on a solid support in accordance with conventional techniques. Various automatic synthesizers are commercially available and can be used in accordance with known 20 protocols. See, for example, Stewart and Young (supra); Tam et al., J Am. Chem. Soc., 105:6442, (1983); Merrifield, Science 232:341-347 (1986); Barany and Merrifield, The Peptides, Gross and Meienhofer, eds, Academic Press, New York, 1-284; Barany et al., Int. J. Pep. Protein Res., 30:705-739 (1987); and U.S. Patent No. 5,424,398. Solid phase peptide synthesis methods use a copoly(styrene-divinylbenzene) 25 containing 0.1-1.0 mM amines/g polymer. These methods for peptide synthesis use butyloxycarbonyl (t-BOC) or 9-fluorenylmethyloxy-carbonyl(FMOC) protection of alpha-amino groups. Both methods involve stepwise syntheses whereby a single amino acid is added at each step starting from the C-terminus of the peptide (See, Coligan et al., Curr. Prot. Immunol., Wiley Interscience, 1991, Unit 9). On completion of 30 chemical synthesis, the synthetic peptide can be deprotected to remove the t-BOC or FMOC amino acid blocking groups and cleaved from the polymer by treatment with acid at reduced temperature (e.g., liquid HF-10% anisole for about 0.25 to about 1 hours at 0*C). After evaporation of the reagents, the peptides are extracted from the polymer 5 with 1% acetic acid solution that is then lyophilized to yield the crude material. This can normally be purified by such techniques as gel filtration on Sephadex G 15 using 5% acetic acid as a solvent. Lyophilization of appropriate fractions of the column will yield the homogeneous peptides or peptide derivatives, which can then be characterized by such standard techniques as amino acid analysis, thin 10 layer chromatography, high performance liquid chromatography, ultraviolet absorption spectroscopy, molar rotation, solubility, and quantitated by the solid phase Edman degradation. Other methods, such as selecting peptides from a phage display library, are also available. Libraries can be prepared from sets of amino acids as described 15 herein. Phage display can be particularly effective in identifying peptides useful according to the invention. Briefly, one prepares a phage library (using e.g. ml 13, fd, or lambda phage), displaying inserts from 4 to about 80 amino acid residues. The inserts may represent, for example, a completely. degenerate or biased array. One then can select phage-bearing inserts that bind to the desired antigen. This 20 process can be repeated through several cycles of reselection of phage that bind to the desired antigen. Repeated rounds lead to enrichment of phage bearing particular sequences. DNA sequence analysis can be conducted to identify the sequences of the expressed peptides. The minimal linear portion of the sequence that binds to the desired antigen can be determined. One can repeat the procedure 25 using a biased library containing inserts containing part or all of the minimal linear portion plus one or more additional degenerate residues upstream or downstream thereof. These techniques may identify peptides of the invention with still.greater binding affinity for Ang-2 than agents already identified herein. Regardless of the manner in which the peptides are prepared, a nucleic 30 acid molecule encoding each such peptide and peptibody can be generated using standard recombinant DNA procedures. The nucleotide sequence of such DNA 74 molecules can be manipulated as appropriate without changing the amino acid sequence they encode to account for the degeneracy of the nucleic acid code as well'as to account for codon preference in particular host cells. Recombinant DNA techniques are a convenient method for preparing full 5 length peptibodies and other large proteinaceous specific binding agents of the present invention, or fragments thereof. A DNA molecule encoding the peptibody or fragment may be inserted into an expression vector, which can in turn be inserted into a host cell for production of the antibody or fragment. Generally, a DNA molecule encoding a peptide or peptibody can be 10 obtained using procedures described herein in the Examples. Probes and typical hybridization conditions are those such as set forth in Ausubel et al. (Current Protocols in Molecular Biology, Current Protocols Press [1994]). After hybridization, the probed blot can be washed at a suitable stringency, depending on such factors as probe size, expected homology of probe to clone, the type of 15 library being screened, and the number of clones being screened. Examples of high stringency screening are 0.1 X SSC, and 0.1 percent SDS at a temperature between 50-65*C. Yeast two-hybrid screening methods also may be used to identify peptides of the invention that bind to the Ang-2. Thus, antigen, or a fragment thereof, can 20 be used to screen peptide libraries, including phage display libraries, to identify and select Ang-2 binding agents, e.g. peptibodies, of the present invention. Alternatively, a variety of expression vector/host systems may be utilized to contain and express the peptides of the invention. These systems include but are not limited to microorganisms such as bacteria transformed with recombinant 25 bacteriophage, plasmid or cosmid DNA expression vectors; yeast transformed with yeast expression vectors; insect cell systems infected with virus expression vectors (e.g., baculovirus); plant cell systems transfected with virus expression vectors (e.g., cauliflower mosaic virus, CaMV; tobacco mosaic virus, TMV) or transformed with bacterial expression vectors (e.g., Ti or pBR322 plasmid); or 30 animal cell systems. Mammalian cells that are useful in recombinant protein productions include but are not limited to VERO cells, HeLa cells, Chinese 75 hamster ovary (CHO) cell lines, COS cells (such as COS-7), W138, BIHK, HepG2, 3T3, RIN, MDCK, A549, PC12, K562 and 293 cells. Exemplary protocols for the recombinant expression of the peptides are described herein below. 5 The term "expression vector" refers to a plasmid, phage, virus or vector, for expressing a polypeptide from a DNA (RNA) sequence. An expression vector can comprise a transcriptional unit comprising an assembly of (1) a genetic element or elements having a regulatory role in gene expression, for example, promoters or enhancers, (2) a structural or sequence that encodes the binding 10 agent which is transcribed into mRNA and translated into protein, and (3) appropriate transcription initiation and termination sequences. Structural units intended for use in yeast or eukaryotic expression systems preferably include a leader sequence enabling extracellular secretion of translated protein by a host cell. Alternatively, where recombinant protein is expressed without a leader or 15 transport sequence, it may include an amino terminal methionyl residue. This residue may or may not be subsequently cleaved from the expressed recombinant protein to provide a final peptide product. For example, the peptides may be recombinantly expressed in yeast using a commercially available expression system, e.g., the Pichia Expression System 20 (Invitrogen, San Diego, CA), following the manufacturer's instructions. This system also relies on the pre-pro-alpha sequence to direct secretion, but transcription of the insert is driven by the alcohol oxidase (AOX1) promoter upon induction by methanol. The secreted peptide is purified from the yeast growth medium by, e.g., 25 the methods used to purify the peptide from bacterial and mammalian cell supernatants.

Alternatively, the cDNA encoding the peptide may be cloned into the baculovirus expression vector pVL1393 (PharMingen, San Diego, CA). This vector can be used according to the manufacturer's directions (PharMingen) to 30 infect Spodoptera frugiperda cells in sF9 protein-free media and to produce 76 - 77 recombinant protein. The recombinant protein can be purified and concentrated from the media using a heparin-Sepharose column (Pharmacia). Alternatively, the peptide may be expressed in an insect system. Insect systems for protein expression are well known to those of skill in the art. In one such system, 5 Autographa californica nuclear polyhedrosis virus (AcNPV) can be used as a vector to express foreign genes in Spodopterafrugiperda cells or in Trichoplusia larvae. The peptide coding sequence can be cloned into a nonessential region of the virus, such as the polyhedrin gene, and placed under control of the polyhedrin promoter. Successful insertion of the peptide will render the polyhedrin gene inactive and produce recombinant virus 10 lacking coat protein. The recombinant viruses can be used to infect S. frugiperda cells or Trichoplusia larvae in which the peptide is expressed. Smith et al., J. Virol. 46: 584 (1983); Engelhard et al., Proc. Nat. Acad. Sci. (USA) 91: 3224-7 (1994). In another example, the DNA sequence encoding the peptide can be amplified by PCR and cloned into an appropriate vector, for example, pGEX-3X (Pharmacia). The pGEX 15 vector is designed to produce a fusion protein comprising glutathione-S-transferase (GST), encoded by the vector, and a protein encoded by a DNA fragment inserted into the vector's cloning site. The primers for PCR can be generated to include, for example, an appropriate cleavage site. Where the fusion moiety is used solely to facilitate expression or is otherwise not desirable as an attachment to the peptide of interest, the recombinant fusion protein may 20 then be cleaved from the GST portion of the fusion protein. The pGEX-3X/specific binding agent peptide construct is transformed into E. coli XL-I Blue cells (Stratagene, La Jolla CA), and individual transformants isolated and grown. Plasmid DNA from individual transformants can be purified and partially sequenced using an automated sequencer to confirm the presence of the desired specific binding agent encoding nucleic acid insert in the 25 proper orientation. Certain peptide compositions of the present invention are those in which a peptibody is conjugated to any anti-tumor peptide such as tumor necrosis factor (TNF). In a particularly preferred method, the TNF-specific binding agent peptides chimeras are generated as recombinant fusions with peptide-encoding sequences fused in frame to TNF (Novagen, Madison, WI) encoding sequences. Peptide-TNF cDNA can be cloned into pET-1lb vector (Novagen) and the expression of TNF-peptides in BL21 E. coli can be induced according to the 5 pET1 lb manufacturer's instruction. Soluble TNF-peptides can be purified from bacterial lysates by ammonium sulfate preparation, hydrophobic interaction chromatography on Phenyl-Sepharose 6 Fast Flow, ion exchange chromatography on DEAB-Sepharose Fast-Flow and gel filtration chromatography on Sephacryl-S 300 HR. 10 The fusion protein, which may be produced as an insoluble inclusion body in the bacteria, can be purified as follows. Host cells can be sacrificed by centrifugation; washed in 0.15 M NaCl, 10 mM Tris, pH 8, 1 mM EDTA; and treated with 0.1 mg/ml lysozyme (Sigma, St. Louis, MO) for 15 minutes at room temperature. The lysate can be cleared by sonication, and cell debris can be 15 pelleted by centrifugation for 10 minutes at 12,000 X g. The fusion protein containing pellet can be resuspended in 50 mM Tris, pH 8, and 10 mM EDTA, layered over 50% glycerol, and centrifuged for 30 minutes at 6000 X g. The pellet can be resuspended in standard phosphate buffered saline solution (PBS) free of Mg++ and Ca++. The fusion protein can be further purified by 20 fractionating the resuspended pellet in a denaturing SDS-PAGE (Sambrook et al., supra). The gel can be soaked in 0.4 M KCl to visualize the protein, which can be excised and electroeluted in gel-running buffer lacking SDS. If the GST/fusion protein is produced in bacteria as a soluble protein, it can be purified using the GST Purification Module (Pharmacia). 25 The fusion protein may be subjected to digestion to cleave the GST from the peptide of the invention. The digestion reaction (20-40 mg fusion protein, 20 30 units human thrombin (4000 U/mg, Sigma) in 0.5 ml PBS can be incubated 16 48 hrs at room temperature and loaded on a denaturing SDS-PAGE gel to fractionate the reaction products. The gel can be soaked in 0.4 M KC1 to visualize 30 the protein bands. The identity of the protein band corresponding to the expected molecular weight of the peptide can be confirmed by amino acid sequence 78 analysis using an automated sequencer (Applied Biosystems Model 473A, Foster City, CA). Alternatively, the identity can be confirmed by performing HPLC and/or mass spectometry of the peptides. Alternatively, a DNA sequence encoding the peptide can be cloned into a 5 plasmid containing a desired promoter and, optionally, a leader sequence [Better et al., Science 240:1041-43 (1988)]. The sequence of this construct can be confirmed by automated sequencing. The plasmid can then be transformed into E. coli strain MC1061 using standard procedures employing CaCl2 incubation and heat shock treatment of the bacteria (Sambrook et al., supra). The transformed 10 bacteria can be grown in LB medium supplemented with carbenicillin, and production. of the expressed protein can be induced by growth in a suitable medium.. If present, the leader sequence can effect secretion of the peptide and be cleaved during secretion. The secreted recombinant protein can be purified from the bacterial culture 15 media by the methods described herein below. Mammalian host systems for the expression of the recombinant protein are well known to those of skill in the art. Host cell strains can be chosen for a particular ability to process the expressed protein or produce certain post translation modifications that will be useful in providing protein activity. Such 20 modifications of the protein include, but are not limited to, acetylation, carboxylation, glycosylation, phosphorylation, lipidation and acylation. Different host cells such as CHO, HeLa, MDCK, 293, W138, and the like have specific cellular machinery and characteristic mechanisms for such post-translational activities and can be chosen to ensure the correct modification and processing of 25 the introduced, foreign protein. It is preferable that the transformed cells be used for long-term, high-yield protein production and as such stable expression is desirable. Once such cells are transformed with vectors that contain selectable markers along with the desired expression cassette, the cells can be allowed to grow for 1-2 days in an enriched 30 media before they are switched to selective media. The selectable marker is designed to confer resistance to selection and its presence allows growth and 79 recovery of cells that successfully express the introduced sequences. Resistant clumps of stably transformed cells can be proliferated using tissue culture techniques appropriate to the cell. A number of selection systems can be used to recover the cells that have 5 been transformed for recombinant protein production. Such selection systems include, but are not limited to, HSV thymidine kinase, hypoxanthine-guanine phosphoribosyltransferase and adenine phosphoribosyltransferase genes, in tk-, hgprt- or aprt- cells, respectively. Also, anti-metabolite resistance can be used as the basis of selection for DHFR which confers resistance to methotrexate; gpt 10 which confers resistance to mycophenolic acid; neo which confers resistance to the aminoglycoside G418 and confers resistance to chlorsulfuron; and hygro which confers resistance to hygromycin. Additional selectable genes that may be useful include trpB, which allows cells to utilize indole in place of tryptophan, or hisD, which allows cells to utilize histinol in place of histidine. Markers that give 15 a visual indication for identification of transformants include anthocyanins,

P

glucuronidase and its substrate, GUS, and luciferase and its substrate, luciferin. Purification and Refolding of Specific Binding Agents 20 In some cases, the specific binding agents such as the peptides and/or peptibodies of this invention may need to be refoldedd" and oxidized into a proper tertiary structure and generating disulfide linkages in order to be biologically active. Refolding can be accomplished using a number of procedures well known in the art. Such methods include, for example, exposing the solubilized 25 polypeptide agent to a pH usually above 7 in the presence of a chaotropic agent. The selection of chaotrope is similar to the choices used for inclusion body solubilization, however a chaotrope is typically used at a lower concentration. An exemplary chaotropic agent is guanidine. In most cases, the refolding/oxidation solution will also contain a reducing agent plus its oxidized form in a specific 30 ratio to generate a particular redox potential which allows for disulfide shuffling to occur for the formation of cysteine bridges. Some commonly used redox 80 -81 couples include cysteine/cystamine, glutathione/dithiobis GSH, cupric chloride, dithiothreitol DTT/dithiane DTT, and 2-mercaptoethanol (bME)/dithio-bME. In many instances, a co-solvent may be used to increase the efficiency of the refolding. Commonly used cosolvents include glycerol, polyethylene glycol of various molecular weights, and 5 arginine. It may be desirable to purify the peptides and peptibodies of the present invention. Protein purification techniques are well known to those of skill in the art. These techniques involve, at one level, the crude fractionation of the proteinaceous and non-proteinaceous fractions. Having separated the peptide and/or peptibody from other proteins, the peptide or 10 polypeptide of interest can be further purified using chromatographic and electrophoretic techniques to achieve partial or complete purification (or purification to homogeneity). Analytical methods particularly suited to the preparation of peptibodies and peptides of the present invention are ion-exchange chromatography, exclusion chromatography; polyacrylamide gel electrophoresis; isoelectric focusing. A particularly efficient method of 15 purifying peptides is fast protein liquid chromatography or even HPLC. Certain aspects of the present invention concern the purification, and in particular embodiments, the substantial purification, of a peptibody or peptide of the present invention. The term "purified peptibody or peptide" as used herein, is intended to refer to a composition, isolatable from other components, wherein the peptibody or peptide is purified 20 to any degree relative to its naturally-obtainable state. A purified peptide or peptibody therefore also refers to a peptibody or peptide that is free from the environment in which it may naturally occur. Generally, "purified" will refer to a peptide or peptibody composition that has been subjected to fractionation to remove various other components, and which composition 25 substantially retains its expressed biological activity. Where the term "substantially purified" is used, this designation will refer to a peptide or peptibody composition in which the peptibody or peptide forms the major component of the composition, such as constituting about 50%, about 60%, about 70%, about 80%, about 90%, about 95% or more of the proteins in the composition.

Various methods for quantifying the degree of purification of the peptide or peptibody will be known to those of skill in the art in light of the present disclosure. These include, for example, determining the specific binding activity of an active fraction, or. assessing the amount of peptide or peptibody within a 5 fraction by SDS/PAGE analysis. A preferred method for assessing the purity of a peptide or peptibody fraction is to calculate the binding activity of the fraction, to compare it to the binding activity of the initial extract, and to thus calculate the degree of purification, herein assessed by a "-fold purification number." The actual units used to represent the amount of binding activity will, of course, be 10 dependent upon the particular assay technique chosen to follow the purification and whether or not the peptibody or peptide exhibits a detectable binding activity. Various techniques suitable for use in purification will be well known to those of skill in the art. These include, for example, precipitation with ammonium - sulphate, PEG, antibodies (immunoprecipitation) and the like or by heat 15 denaturation, followed by centrifugation; chromatography steps such as affinity chromatography (e.g., Protein-A-Sepharose), ion exchange, gel filtration, reverse phase,. hydroxylapatite and affinity chromatography; isoelectric focusing; gel electrophoresis; and combinations of such and other techniques. As is generally known in the art, it is believed that the order of conducting the various purification 20 steps may be changed, or that certain steps may be omitted, and still result in a suitable method for the preparation of a substantially purified specific binding agent. There is no general requirement that the peptide or peptibody of the present invention always be provided in its most purified state. Indeed, it is 25 contemplated that less substantially specific binding agent products will have utility in certain embodiments. Partial purification may be accomplished by using fewer purification steps in combination, or by utilizing different forms of the same general purification scheme. For example, it is appreciated that a cation-exchange column chromatography performed utilizing an [PLC apparatus will generally 30 result in a greater "-fold" purification than the same technique utilizing a low pressure chromatography system. Methods exhibiting a lower degree of relative 82 purification may have advantages in total recovery of the peptide or peptibody, or in maintaining binding activity of the peptide or peptibody. It is known that the migration of a peptide or polypeptide can vary, sometimes significantly, with different conditions of SDS/PAGE [Capaldi et al., 5 Biochem. Biophys. Res. Comm., 76: 425 (1977)]. It will therefore be appreciated that under differing electrophoresis conditions, the apparent molecular weights of purified or partially purified specific binding agent expression products may vary. 10 Binding Assays Immunological binding assays typically utilize a capture agent to bind specifically to and often immobilize the analyte target antigen. The capture agent is a moiety that specifically binds to the analyte. In one embodimnent of the present invention, the capture agent is a peptide or peptibody or fragment thereof 15 that specifically binds Ang-2. These immunological binding assays are well known in the att [Asai, ed., Methods in Cell Biology, Vol. 37, Antibodies in Cell Biology, Academic Press, Inc., New York (1993)]. Immiiunological binding assays frequently utilize a labeling agent that w1l signal the existence of the bound complex formed by the capture agent and 20 antigen. The labeling agent can be one of the molecules comprising the bound complex; i.e. it can be a labeled specific binding agent or a labeled anti-specific binding agent antibody.- Alternatively, the labeling agent can be a third molecule, commonly another antibody, which binds to the bound complex. The labeling agent can be, for example, an anti-specific binding agent antibody bearing a label. 25 The second antibody, specific for the bound complex, may lack a label, but can be bound by a fourth molecule specific to the species of antibodies which the second antibody is a member of. For example, the second antibody can be modified with a detectable moiety, such as biotin, which can then be bound by a fourth molecule, such as enzyme-labeled'streptavidin. Other proteins capable of specifically 30 binding immunoglobulin constant regions, such as protein A or protein G may also be used as the labeling agent. These binding proteins are normal constituents of the cell walls of streptococcal bacteria and exhibit a strong non-immunogenic 83 - 84 reactivity with immunoglobulin constant regions from a variety of species. Akerstrom, J. Immunol., 135:2589-2542 (1985); Chaubert, Mod. Pathol., 10:585-591 (1997). Throughout the assays, incubation and/or washing steps may be required after each combination of reagents. Incubation steps can vary from about 5 seconds to several 5 hours, preferably from about 5 minutes to about 24 hours. However, the incubation time will depend upon the assay format, analyte, volume of solution, concentrations, and the like. Usually, the assays will be carried out at ambient temperature, although they can be conducted over a range of temperatures. 10 A. Non-competitive binding assays: Immunological binding assays can be of the non-competitive type. These assays have an amount of captured analyte that is directly measured. For example, in one preferred "sandwich" assay, the capture agent (antibody or peptibody) can be bound directly to a solid substrate where it is immobilized. These immobilized capture agents 15 then capture (bind to) antigen present in the test sample. The protein thus immobilized is then bound to a labeling agent, such as a second antibody having a label. In another preferred "sandwich" assay, the second antibody lacks a label, but can be bound by a labeled antibody specific for antibodies of the species from which the second antibody is derived. The second antibody also can be modified with a detectable moiety, such as 20 biotin, to which a third labeled molecule can specifically bind, such as streptavidin. See Harlow and Lane, Antibodies, A Laboratory Manual, Ch 14, Cold Spring Harbor Laboratory, NY (1988). B. Competitive Binding Assays: 25 Immunological binding assays can be of the competitive type. The amount of analyte present in the sample is measure indirectly by measuring the amount of an added analyte displaced, or competed away, from a capture agent (antibody or peptibody) by the analyte present in the sample. In one preferred competitive binding assay, a known amount of analyte, usually labeled, is added to the sample and the sample is then contacted with the capture agent. The amount of labeled analyze bound to the antibody is inversely proportional to the concentration of analyte present in the sample (See, Harlow and Lane, Antibodies, 5 A Laboratory Manual, Ch 14, pp. 579-583, supra). In another preferred competitive binding assay, the capture agent is immobilized on a solid substrate. The amount of protein bound to the capture agent may be determined either by measuring the amount of protein present in a protein/antibody complex, or alternatively by measuring the amount of remaining 10 uncomplexed protein. The amount of protein may be detected by providing a labeled protein. Harlow and Lane (supra). Yet another preferred competitive binding assay, hapten inhibition is utilized. Here, a known analyte is immobilized on a solid substrate. A known amount of antibody is added to the sample, and the sample is contacted with the 15 immobilized analyte. The amount of antibody bound to the immobilized analyte is inversely proportional to the amount of analyte present in the sample. The amount of immobilized antibody may be detected by detecting either the immobilized fraction of antibody or the fraction that remains in solution. Detection may be direct where the antibody is labeled or indirect by the 20 subsequent addition of a labeled moiety that specifically binds to the antibody as described above. C. Utilization of Competitive Binding Assays: The competitive binding assays can bemused for cross-reactivity 25 determinations to permit a skilled artisan to determine if a protein or enzyme complex which is recognized by a peptibody of the invention is the desired protein and not a cross-reacting molecule or to determine whether the peptibody is specific for the antigen and does not bind unrelated antigens. In assays of this type, antigen can be immobilized to a solid support and an unknown protein 30 mixture is added to the assay, which will compete with the binding of the peptibodies to the immobilized protein. The competing molecule also binds one or 85 more antigens unrelated to the antigen. The ability of the proteins to compete with the binding of the peptibodies to the immobilized antigen is compared to the binding by the same protein that was immobilized to the solid support to determine the cross-reactivity of the protein mix. 5 D. Other Binding Assays The present- invention also provides Western blot methods to detect or quantify the presence of Ang-2 in a sample. The technique generally comprises separating sample proteins by gel electrophoresis on the basis of molecular weight .10 and transferring the proteins to a suitable solid support, such as nitrocellulose filter, a nylon filter, or derivatized nylon filter. The sample is incubated with peptibodies or fragments thereof that specifically bind Ang-2 and the resulting complex is detected. These peptibodies may be directly labeled or alternatively may be subsequently detected using labeled antibodies that specifically bind to the 15 peptibody. Diagnostic Assays The derivative binding agents, such as peptides and peptibodies or 20 fragments thereof, of the present invention are useful for the diagnosis of conditions or diseases characterized by expression of Ang-2 or subunits, or in assays to monitor patients being treated with inducers of Ang-2, its fragments, agonists or inhibitors of Ang-2 activity. Diagnostic assays for Ang-2 include methods utilizing a peptibody and a label to detect Ang-2 in human body fluids or 25 extracts of cells or tissues. The peptibodies of the present invention can be used with or without modification. In a preferred diagnostic assay, the peptibodies will be labeled by attaching, e.g., a label or a reporter molecule. A wide variety of labels and reporter molecules are known, some of which have been already described herein. In particular, the present invention is useful for diagnosis of 30 human disease. A variety of protocols for measuring Ang-2 proteins using peptibodies specific for the respective protein are known in the art. Examples include 86 enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA) and fluorescence activated cell sorting (FACS). A two-site, monoclonal-based immunoassay utilizing monoclonal antibodies reactive to two non-interfering epitopes on Ang-2 is preferred, but a competitive binding assay can be employed. 5 These assays are described, for example, in Maddox et al., J. Exp. Med., 158:1211 (1983). In order to provide a basis for diagnosis, normal or standard values for human Ang-2 expression are usually established.. This determination can be accomplished by combining body fluids or cell extracts from normal subjects, 10 preferably human, with a peptibody to Ang-2, under conditions suitable for complex formation that are well known in the art. The amount of standard complex formation can be quantified by comparing the binding of the peptibodies to known quantities of Ang-2 protein, with both control and disease samples. Then, standard values obtained from normal- samples can be compared with values 15 obtained from samples from subjects potentially affected by disease. Deviation between standard and subject values suggests a role for Ang-2 in the disease state. For diagnostic applications, in certain embodiments peptibodies or peptides of the present invention typically will be labeled with a detectable moiety. The detectable moiety can be any one that is capable of producing, either 20 directly or indirectly, a detectable signal. For example, the detectable moiety may be a radioisotope, such as 3 H, 1 4 c, 32 P, 35 S, or 1251, a fluorescent or chemiluminescent compound, such as fluorescein isothiocyanate, rhodamine, or luciferin; or an enzyme, such as alkaline phosphatase, #-galactosidase, or horseradish peroxidase. Bayer et al., Meth. Enz., 184: 138-163, (1990). 25 Diseases The present invention provides a binding agent such as a peptide, peptibody, or fragment, variant or derivative thereof that binds to Ang-2 that is 30 useful for the treatment of human diseases and pathological conditions. Agents that modulate Ang-2 binding activity, or other cellular activity, may be used in 87 combination with other therapeutic agents to enhance their therapeutic effects or decrease potential side effects. . In one aspect, the present invention provides reagents and methods useful for treating diseases and conditions characterized by undesirable or aberrant levels 5 of Ang-2 activity in a cell. These diseases include cancers, and other hyperproliferative conditions, such as hyperplasia, psoriasis, contact dermatitis, immunological disorders, and infertility. The present invention also provides methods of treating cancer in an animal, including humans, comprising administering to the animal an effective 10 amount of a specific binding agent, such as a peptibody, that inhibits or decreases Ang-2 activity. The invention is further directed to methods of inhibiting cancer cell growth, including processes of cellular proliferation, invasiveness, and metastasis in biological systems. Methods include use of a compound of the invention as an inhibitor of cancercell growth. Preferably,. the methods are 15 employed to inhibit or reduce cancer cell growth, invasiveness, metastasis, or tumor incidence in living animals, such as mammals. Methods of the invention are also readily adaptable for use in assay systems, e.g., assaying cancer cell growth and properties thereof, as well as identifying compounds that affect cancer cell growth. 20 The cancers treatable by methods of the present invention preferably occur in mammals. Mammals include, for example, humans and other primates, as well as pet or companion animals such as dogs and cats, laboratory animals such as rats, mice and rabbits, and farm animals such as horses, pigs, sheep, and cattle. Tumors or neoplasms include growths of tissue cells in which the 25 multiplication of the cells is uncontrolled and progressive. Some such growths are benign, but others are termed malignant and may lead to death of the organism. Malignant neoplasms or cancers are distinguished from benign growths in that, in addition to exhibiting aggressive cellular proliferation, they may invade surrounding tissues and metastasize. Moreover, malignant neoplasms 30 are characterized in that they show a greater loss of differentiation (greater 88 dedifferentiation), and of -their organization relative to one another and their surrounding tissues. This property is also called "anaplasia." Neoplasms treatable by the present invention also include solid tumors, i.e., carcinomas and sarcomas. Carcinomas include those malignant neoplasms 5 derived from epithelial cells that infiltrate (invade) the surrounding tissues and give rise to metastases. Adenocarcinomas are carcinomas derived from glandular tissue, or which form recognizable glandular structures. Another broad category or cancers includes sarcomas, which are tumors whose cells are embedded in a fibrillar or homogeneous substance like embryonic connective tissue. The 10 invention also enables treatment of cancers of the myeloid or lymphoid systems, including leukemias, lymphomas and other cancers that typically do not present as a tumor mass, but are distributed in the vascular or lymphoreticular systems. The type of cancer or tumor cells amenable to treatment according to the invention include, for example, ACTH-producing tumor, acute lymphocytic 15 leukemia, acute nonlymphocytic leukemia, cancer of the adrenal cortex, bladder cancer, brain cancer, breast cancer, cervical cancer, chronic lymphocytic leukemia, chronic miyelocytic leukemia, colorectal cancer, cutaneous T-cell lymphoma, endometrial cancer, esophageal cancer, Ewing's sarcoma, gallbladder cancer, hairy cell leukemia, head and neck cancer, Hodgkin's lymphoma, Kaposi's 20 sarcoma, kidney cancer, liver cancer, lung cancer (small and non-small cell), malignant peritoneal effusion, malignant pleural effusion, melanoma, mesothelioma, multiple myeloma, neuroblastoma, glioma, non-Hodgkin's lymphoma, osteosarcoma, ovarian cancer, ovarian (germ cell) cancer, pancreatic cancer, penile dancer, prostate cancer, retinoblastoma, skin cancer, soft tissue 25 sarcoma, squamous cell carcinomas, stomach cancer, testicular cancer, thyroid cancer, trophoblastic neoplasms, uterine cancer, vaginal cancer, cancer of the vulva, and Wilms' tumor. The invention is particularly illustrated herein in reference to treatment of certain types of experimentally defined cancers. In these illustrative treatments, 30 standard state-of-the-art in vitro and in vivo models have been used. These methods can be used to identify agents that can be expected to be efficacious in in 89 vivo treatment regimens. However, it will be understood that the method of the invention is not limited to the treatment of these tumor types, but extends to any solid tumor derived from any organ system. Cancers whose invasiveness or metastasis is associated with Ang-2 expression or activity are especially .5 susceptible to being inhibited or even induced to regress by means of the invention. The invention can also be practiced by including with a compound of the - invention such as a peptibody in combination with another anti-cancer chemotherapeutic agent, such as any conventional chemotherapeutic agent. The 10 combination of a specific binding agent with such. other agents can potentiate the chemotherapeutic protocol. Numerous chemotherapeutic protocols will present themselves in the mind of the skilled practitioner as being capable of incorporation into the method of the invention. Any chemotherapeutic agent can be used, including alkylating agents, antimetabolites, honnones and antagonists, 15 radioisotopes, as well as natural products. For example, the compound of the invention can be administered with antibiotics such- as doxorubicin and other anthracycline analogs, nitrogen mustards such as cyclophosphamide, pyrimidine analogs such as 5-fluorouracil, cisplatin, hydroxyurea, taxol and its natural and synthetic derivatives, and the like. As another example, in the case of mixed 20 tumors, such as adenocarcinoma of the breast, where the tumors include gonadotropin-dependent and gonadotropin-independent cells, the compound can be administered in conjunction with leuprolide or goserelin (synthetic peptide analogs of LH-RH). Other antineoplastic protocols include the use of a tetracycline compound with another treatment modality, e.g., surgery, radiation, 25 etc., also referred to herein as "adjunct antineoplastic modalities." Thus, the method of the invention can be employed with such conventional regimens with the benefit of reducing side effects and enhancing efficacy. The present invention thus provides compositions and methods useful for the treatment of a wide variety of cancers, including solid tumors and leukemias. 30 Types of cancer that may be treated include, but are not limited to: adenocarcinoma of the breast, prostate, and colon; all forms of bronchogenic 90 -91 carcinoma of the lung; myeloid; melanoma; hepatoma; neuroblastoma; papilloma; apudoma; choristoma; branchioma; malignant carcinoid syndrome; carcinoid heart disease; carcinoma (e.g., Walker, basal cell, basosquamous, Brown-Pearce, ductal, Ehrlich tumor, Krebs 2, merkel cell, mucinous, non-small cell lung, oat cell, papillary, scirrhous, bronchiolar, 5 bronchogenic, squamous cell, and transitional cell); histiocytic disorders; leukemia; histiocytosis malignant; Hodgkin's disease; immunoproliferative small lung cell carcinoma; non-Hodgkin's lymphoma; plasmacytoma; reticuloendotheliosis; melanoma; chondroblastoma; chondroma; chondrosarcoma; fibroma; fibrosarcoma; giant cell tumors; histiocytoma; lipoma; liposarcoma; mesothelioma; myxoma; myxosarcoma; osteoma; 10 osteosarcoma; chordoma; craniopharyngioma; dysgerminoma; hamartoma; mesenchymoma; mesonephroma; myosarcoma; ameloblastoma; cementoma; odontoma; teratoma; thymoma; trophoblastic tumor. Further, the following types of cancers may also be treated: adenoma; cholangioma; cholesteatoma; cyclindroma; cystadenocarcinoma; cystadenoma; granulosa cell tumor; gynandroblastoma; hepatoma; hidradenoma; islet cell tumor; Leydig cell tumor; 15 papilloma; Sertoli cell tumor; theca cell tumor; leiomyoma; leiomyosarcoma; myoblastoma; myoma; myosarcoma; rhabdomyoma; rhabdomyosarcoma; ependymoma; ganglioneuroma; glioma; medulloblastoma; meningioma; neurilemmoma; neuroblastoma; neuroepithelioma; neurofibroma; neuroma; paraganglioma; paraganglioma nonchromaffin; angiokeratoma; angiolymphoid hyperplasia with eosinophilia; angioma sclerosing; angiomatosis; 20 glomangioma; hemangioendothelioma; hemangioma; hemangiopericytoma; hemangiosarcoma; lymphangioma; lymphangiomyoma; lymphangiosarcoma; pinealoma; carcinosarcoma; chondrosarcoma; cystosarcoma phyllodes; fibrosarcoma; hemangiosarcoma; leiomyosarcoma; leukosarcoma; liposarcoma; lymphangiosarcoma; myosarcoma; myxosarcoma; ovarian carcinoma; rhabdomyosarcoma; sarcoma; neoplasms; 25 nerofibromatosis; and cervical dysplasia. Another aspect of the present invention is using the materials and methods of the present invention to prevent and/or treat any hyperproliferative condition of the skin including psoriasis and contact dermatitis or other hyperproliferative diseases.. It has been demonstrated that patients with psoriasis and contact dermatitis have elevated Ang-2 activity within these lesions [Ogoshi et al., J. Inv. Dennatol., 110:818-23 (1998)]. Preferably, specific binding agents specific for Ang-2 will be used in combination with other phannaceutical agents to treat 5 humans that express these clinical symptoms. The specific binding agents can be delivered using any of the various carriers through routes of administration described herein and others that are well known to those of skill in the art. Other aspects of the present invention include treating various retinopathies (including diabetic retinopathy and age-related macular 10 degeneration) in which angiogenesis is involved, as well as disorders/diseases of the female reproductive tract such as endometriosis, uterine fibroids, and other such conditions associated with dysfunctional vascular proliferation (including endometrial microvascular growth) during the female reproductive cycle. Still another aspect of the present invention relates to treating abnormal 15 vascular growth including cerebral arteriovenous malformations (AVMs) gastrointestinal mucosal injury and repair, ulceration of the gastroduodenal mucosa in patients with a history of peptic ulcer disease; including ischemia resulting from stroke, a wide spectrum of pulmonary vascular disorders in liver disease and portal hypertension in patients with nonhepatic portal hypertension. 20 Another aspect of present invention is the prevention of cancers utilizing the compositions and methods provided by the present invention. Such reagents will include specific binding, agents such as peptibodies against Ang-2. Pharmaceutical Compositions 25 Pharmaceutical compositions of Ang-2 specific binding agents such as peptibodies are within the scope of the present invention. Pharmaceutical compositions comprising antibodies are described in detail in, for example, US Patent 6,171,586, to Lam et al., issued January 9, 2001. Such compositions comprise a therapeutically or prophylactically effective amount of a specific 30 binding agent, such as an antibody, or a fragment, variant, derivative or fusion thereof as described herein, in admixture with a pharmaceutically acceptable 92 agent. In a preferred embodiment, pharmaceutical compositions comprise antagonist specific binding agents that modulate partially or completely at least one biological activity of Ang-2 in admixture with a pharmaceutically acceptable agent. Typically, the specific binding agents will be sufficiently purified for 5 administration to an animal. The pharmaceutical composition may contain formulation materials for modifying, maintaining or preserving, for example, the pH, osmolarity, viscosity, -clarity, color, isotonicity, odor, sterility, stability, rate of dissolution or release, adsorption or penetration of the composition. Suitable formulation materials 10 include, but are not limited to, amino acids (such as glycine; glutamine, asparagine, arginine or lysine); antimicrobials; antioxidants (such as ascorbic acid, sodium sulfite or sodium hydrogen-sulfite); buffers (such as borate,-bicarbonate, Tris-HCl, citrates, phosphates, other organic acids); bulking agents (such as mannitol or glycine), chelating agents [such as ethylenediamine tetraacetic acid 15 (EDTA)]; complexing agents (such as caffeine, polyvinylpyrrolidone, beta cyclodextrin or hydroxypropyl-beta-cyclodextrin); fillers; monosaccharides; disaccharides and other carbohydrates (such as glucose, mannose, or dextrins); proteins (such as serum albumin, gelatin or immunoglobulins);-coloring; flavoring and diluting agents; emulsifying agents; hydrophilic polymers (such as 20 polyvinylpyrrolidone); low molecular weight polypeptides; salt-forming counterions (such as sodium); preservatives (such as benzalkonium chloride, benzoic acid, salicylic acid, thimerosal, phenethyl alcohol, methylparaben, propylparaben, chlorhexidine, sorbic acid or hydrogen peroxide); solvents (such as glycerin, propylene glycol or polyethylene glycol); sugar alcohols (such -as 25 mannitol or sorbitol); suspending agents; surfactants or wetting agents (such as pluronics, PEG; sorbitan esters, polysorbates such as polysorbate 20, polysorbate 80, triton, tromethamine, lecithin, cholesterol, tyloxapal); stability enhancing agents (sucrose or sorbitol); tonicity enhancing agents (such as alkali metal halides (preferably sodium or potassium chloride, mannitol sorbitol); delivery 30 vehicles; diluents; excipients and/or pharmaceutical adjuvants. (Remington's 93 Pharmaceutical Sciences, 18th Edition, A.R. Gennaro, ed., Mack Publishing Company, 1990). The optimal pharmaceutical composition will be determined by one skilled in the art depending upon, for example, the intended route of administration, 5 delivery format, and desired dosage. See for example, Remington's Pharmaceutical Sciences, supra. Such compositions may influence the physical state, stability, rate of in vivo release, and rate of in vivo clearance of the specific binding agent. The primary vehicle or carrier in a pharmaceutical composition may be 10 either aqueous or non-aqueous in nature. For example, a suitable vehicle or carrier may be water for injection, physiological saline solution or artificial cerebrospinal fluid, possibly supplemented with other materials common in compositions for parenteral administration. Neutral buffered saline or saline mixed with serum albumin are further exemplary vehicles. Other exemplary 15 pharmaceutical compositions comprise Tris buffer of about pH 7.0-8.5, or acetate buffer of about pH 4.0-5.5, which may further include sorbitol or a suitable substitute therefore. In one embodiment of the present invention, binding agent compositions may be prepared for storage by mixing the selected composition having the desired degree of purity with optional formulation agents (Remington's 20 Pharmaceutical Sciences, supra) in the form of a lyophilized cake or an aqueous solution. Further, the binding agent product may be formulated as a lyophilizate using appropriate excipients such as sucrose. . The pharmaceutical compositions can be selected for parenteral delivery. Alternatively, the compositions may be selected for inhalation or for enteral 25 delivery such as orally, aurally, opthalmically, rectally, or vaginally. The preparation of such pharmaceutically acceptable compositions is within the skill of the art. The formulation components are present in concentrations that are acceptable to the site of administration. For example, buffers are used to maintain 30 the composition at physiological pH or at slightly lower pH, typically within a pH range of from about 5 to about 8. 94 When parenteral administration is contemplated, the therapeutic compositions for use in this invention may be in the form of a.pyrogen-free, parenterally acceptable aqueous solution comprising the desired specific-binding agent in a pharmaceutically acceptable vehicle. A particularly suitable vehicle for 5 parenteral injection is sterile distilled water in which a binding agent is formulated as a sterile, isotonic solution, properly preserved. Yet another preparation can involve the formulation of the desired molecule with an agent, such as injectable microspheres, bio-erodible particles, polymeric compounds (polylactic acid, polyglycolic acid), beads, or liposomes, that provides for the controlled or 10 sustained release of the product which may then be delivered via a depot injection. Hyaluronic acid may also be used, and this may have the effect of promoting sustained duration in the circulation. Other suitable means for the introduction of the desired molecule include implantable drug delivery devices. In another aspect, pharmaceutical formulations suitable for parenteral 15 administration may be formulated in aqueous solutions, preferably in physiologically compatible buffers such as Hanks' solution, ringer's solution, or physiologically buffered saline. Aqueous injection suspensions may contain. substances that increase the viscosity of the suspension, such as sodium carboxymnethyl cellulose, sorbitol, or dextran. Additionally, suspensions of the 20 active compounds may be prepared as appropriate oily injection suspensions. Suitable lipophilic solvents or vehicles include fatty oils, such as sesame oil, or synthetic fatty acid esters, such as ethyl oleate, triglycerides, or-liposomes. Non lipid polycationic amino polymers may also be used for delivery. Optionally, the suspension may also contain suitable stabilizers or agents to increase the solubility 25 of the compounds and allow for the preparation of highly concentrated solutions. In another embodiment, a pharmaceutical composition may be formulated for inhalation. For example, a binding agent may be formulated as a dry powder for inhalation. Polypeptide or nucleic acid molecule inhalation solutions may also be formulated with a propellant for aerosol delivery. In yet another embodiment, 30 solutions may be nebulized. Pulmonary administration is further described in 95 PCT Application No. PCT/US94/001875, which describes pulmonary delivery of chemically modified proteins. It is also contemplated that certain formulations may be administered orally. In one embodiment of the present invention, binding agent molecules that 5 are administered in this fashion can be formulated with or without those carriers customarily used in the compounding of solid dosage forms such as tablets and capsules. For example, a capsule may be designed to release the active portion of the formulation at the point in the gastrointestinal tract when bioavailability is maximized and pre-systemic degradation is minimized. Additional agents can be 10 included.to facilitate absorption of the binding agent molecule. Diluents, flavorings, low melting point waxes, vegetable oils, lubricants, suspending agents, tablet disintegrating agents, and binders may also be employed. Pharmaceutical compositions for oral administration can also be formulated using pharmaceutically acceptable carriers well known in the art in 15 dosages suitable for oral administration. Such carriers enable the pharmaceutical compositions to be formulated as tablets, pills, dragees, capsules, liquids, gels, syrups, slurries, suspensions, and the like, for ingestion by. the patient. Pharmaceutical preparations for oral use can be obtained through combining active compounds with solid excipient and processing the resultant 20 mixture of granules (optionally, after grinding) to obtain tablets or dragee cores. Suitable auxiliaries can be added, if desired. Suitable excipients include carbohydrate or protein fillers, such as sugars, including lactose, sucrose, mannitol, and sorbitol; starch from com, wheat, rice, potato, or other plants; cellulose, such as methyl cellulose, hydroxypropylmethyl-cellulose, or sodium 25 carboxymethylcellulose; gums, including arabic and tragacanth; and proteins, such as gelatin and collagen. If desired, disintegrating or solubilizing agents may be added, such as the cross-linked polyvinyl pyrrolidone, agar, and alginic acid or a salt thereof, such as sodium alginate. Dragee cores may be used in conjunction with suitable coatings, such as 30 concentrated sugar solutions, which may also contain gum arabic, talc, polyvinylpyrrolidone, carbopol gel, polyethylene glycol, and/or titanium dioxide, 96 lacquer solutions, and suitable organic solvents or solvent mixtures. Dyestuffs or pigments may be added to the tablets or dragee coatings for product identification or to characterize the quantity of active compound, i.e.; dosage. Pharmaceutical preparations that can be used orally also include push-fit 5 capsules made of gelatin, as Well as soft, sealed capsules made of gelatin and a coating,- such as glycerol or sorbitol. Push-fit capsules can contain active ingredients mixed with fillers or binders, such as-lactose or starches, lubricants, such as talc or magnesium stearate, and, optionally, stabilizers. In soft capsules, the active compounds may be dissolved or suspended in suitable liquids, such as 10 fatty oils, liquid, or liquid polyethylene glycol with or without stabilizers. Another pharmaceutical composition may involve an effective quantity of binding agent in a mixture: with non-toxic excipients that are suitable for the manufacture of tablets. By dissolving the tablets in sterile water, or other appropriate vehicle, solutions can be prepared in unit dose form. Suitable 15 excipients include, but are not limited to, inert diluents, such as calcium. carbonate, sodium carbonate or bicarbonate, lactose, or calcium phosphate; or binding agents, such as starch, gelatin, or acacia; or lubricating agents such as magnesium stearate, stearic acid, or talc. Additional pharmaceutical compositions will be evident to those skilled in 20 the art, including formulations involving binding agent molecules in sustained- or controlled-delivery formulations. Techniques for formulating a variety of other sustained- or controlled-delivery means, such as liposome carriers, bio-erodible microparticles or porous beads and depot injections, are also known to those skilled in the art. See for example, PCT/US93/00829 that describes controlled 25 release of porous polymeric microparticles for the delivery of pharmaceutical compositions. Additional examples of sustained-release preparations include semipermeable polymer matrices in the form of shaped articles, e.g. films, or microcapsules. Sustained release matrices may include polyesters, hydrogels, polylactides (U.S. 3,773,919, EP 58,481), copolymers of L-glutamic acid and 30 gamma ethyl-L-glutamate [Sidman et al., Biopolyners, 22:547-556 (1983)], poly

(

2 -hydroxyethyl-methacrylate) [Langer et al., J. Biozed. Mater. Res., 15:167-277, 97 (1981)] and [Langer et al., Chem. Tech., 12:98-105(1982)], ethylene vinyl acetate (Langer et al., supra) or poly-D(-)-3-hydroxybutyric acid (EP.133,988). Sustained-release compositions also include liposomes, which can be prepared by any of several methods known in the art. See e.g., Eppstein et al., Proc. Natl. 5 Acad. Sci. (USA), 82:3688-3692 (1985); EP 36,676; EP 88,046; EP 143,949. The pharmaceutical composition to be used for in vivo administration typically must be sterile. This may be accomplished by filtration through sterile filtration membranes. Where the composition is lyophilized, sterilization using this method may be conducted either prior to or following lyophilization and 10 reconstitution. The composition for parenteral administration may be stored in lyophilized form or in solution. In addition, parenteral compositions generally are placed into a container having a sterile access port, for example, an intravenous solution bag or vial having a stopper pierceable by a hypodermic injection needle. Once the pharmaceutical composition has been formulated, it may be 15 stored in sterile vials as a solution, suspension, gel, emulsion, solid, or a dehydrated or lyophilized powder. Such formulations may be stored either in a ready-to-use form or in a form (e.g., lyophilized) requiring reconstitution. prior to administration. In a specific embodiment, the present invention is directed to kits for 20 producing a single-dose administration unit. The kits may each contain both a first container having a dried protein and a second container having an aqueous formulation. Also included within the scope of this invention are kits containing single and multi-chambered pre-filled syringes (e.g., liquid syringes and 1yosyringes). 25 An -effective amount of a pharmaceutical composition to be employed therapeutically will depend, for example, upon the therapeutic context and objectives. One skilled in the art will appreciate that the appropriate dosage levels for treatment will thus vary depending, in part, upon the molecule delivered, the indication for which the binding agent molecule is being used, the route of 30 administration, and the size (body weight, body surface or organ size) and condition (the age and general health) of the patient. Accordingly, the clinician 98 may titer the dosage and modify the -route of administration to obtain the optimal therapeutic effect. A typical dosage may range from about 0.1 mg/kg to up to about 100 mg/kg or more, depending on the factors mentioned above. In other embodiments, the dosage may range from 0.1 mg/kg up to about 100 mg/kg; or 1 5 mg/kg up to about 100 mg/kg; or 5 mg/kg up to about 100 mg/kg. Fo- any compound, the therapeutically effective dose can be estimated initially either in cell culture assays or in animal models such-as mice, rats, rabbits, dogs, pigs, or monkeys. An animal model may also be used to determine the appropriate concentration range and route of administration. Such information 10 can then be used to determine useful doses and routes for administration in humans. The exact dosage will be determined in light of factors related to the subject requiring treatment.. Dosage and administration are adjusted to provide sufficient levels of the active compound or to maintain the desired effect. Factors 15 that may be taken into account include the severity of the disease state, the general health of the subject, the age, weight, and gender of the subject, time and frequency of administration, drug combination(s), reaction sensitivities, and response to therapy. Long-acting pharmaceutical compositions may be administered every 3 to 4 days, every week, or biweekly depending on the half 20 life and clearance rate of the particular formulation. The frequency of dosing will depend upon the pharmacokinetic parameters of the binding agent molecule in the formulation used. Typically, a composition is administered until a dosage is reached that achieves the desired effect. The composition may therefore be administered as a single dose, or as multiple doses 25 (at the same or different concentrations/dosages) over time, or as a continuous infusion. Further refinement of the appropriate dosage is routinely made. Appropriate dosages may be ascertained through use of appropriate dose-response data. The route of administration of the pharmaceutical composition is in accord 30 with known methods, e.g. orally, through injection by intravenous, intraperitoneal, intracerebral (intra-parenchymal), intracerebroventricular, intramuscular, intra 99 ocular, intraarterial, intraportal, intralesional routes, intramedullary, intrathecal, intraventricular, transdermal, subcutaneous, intraperitoneal, intranasal, enteral, topical, sublingual, urethral, vaginal, or rectal means, by sustained release systems or by implantation devices. Where desired, the compositions may be administered 5 by bolus injection or continuously by infusion, or by implantation device. Alternatively or additionally, the composition may be administered locally via implantation of a membrane, sponge, or another appropriate material on .to which the desired molecule has been absorbed or encapsulated. Where an implantation device is used, the device may be implanted into any suitable tissue 10 or organ, and delivery of the desired molecule may be via diffusion, timed-release bolus, or continuous administration. In some cases, it may be desirable to use pharmaceutical compositions in an ex vivo manner. In such instances, cells, tissues, or organs that have been removed from the patient are exposed to the pharmaceutical compositions after 15 which the cells, tissues and/or organs are subsequently implanted back into the patient. In other cases, a binding agent of the present invention such as a peptibody can be delivered by implanting certain cells that have been genetically engineered, using methods such as those described herein, to express and secrete the 20 polypeptide. Such cells may be animal or human cells, and may be autologous, heterologous, or xenogeneic. Optionally, the cells may be immortalized. In order to decrease the chance of an immunological response, the cells may be encapsulated to avoid infiltration of surrounding tissues. The encapsulation materials- are typically biocompatible, semi-permeable polymeric enclosures or 25 membranes that allow the release of the protein product(s) but prevent the destruction of the cells by the patient's immune system or by other detrimental factors from the surrounding tissues. 30 Combination Therapy Specific binding agents of the invention such as peptibodies can be utilized in combination with other therapeutics in the treatment of diseases 100 - 101 associated with Ang-2 expression. These other therapeutics include, for example radiation treatment, chemotherapy, and targeted therapies such as HerceptinTM, RituxanTM, Gleevec

TM

, and the like. Additional combination therapies not specifically listed herein are also within 5 the scope of the present invention. Chemotherapy treatment can employ anti-neoplastic agents including, for example, alkylating agents including: nitrogen mustards, such as mechlor-ethamine, cyclophosphamide, ifosfamide, melphalan and chlorambucil; nitrosoureas, such as carmustine (BCNU), lomustine (CCNU), and semustine (methyl-CCNU); 10 ethylenimines/methylmelamine such as thriethylenemelamine (TEM), triethylene, thiophosphoramide (thiotepa), hexamethylmelamine (HMM, altretamine); alkyl sulfonates such as busulfan; triazines such as dacarbazine (DTIC); antimetabolites including folic acid analogs such as methotrexate and trimetrexate, pyrimidine analogs such as 5-fluorouracil, fluorodeoxyuridine, gemcitabine, cytosine arabinoside (AraC, cytarabine), 5-azacytidine, 15 2,2'-difluorodeoxycytidine, purine analogs such as 6-mercaptopurine, 6-thioguanine, azathioprine, 2'-deoxycoformycin (pentostatin), erythrohydroxynonyladenine (EHNA), fludarabine phosphate, and 2-chlorodeoxyadenosine (cladribine, 2-CdA); natural products including antimitotic drugs such as paclitaxel, vinca alkaloids including vinblastine (VLB), vincristine, and vinorelbine, taxotere, estramustine, and estramustine phosphate; 20 pipodophylotoxins such as etoposide and teniposide; antibiotics such as actimomycin D, daunomycin (rubidomycin), doxorubicin, mitoxantrone, idarubicin, bleomycins, plicamycin (mithramycin), mitomycinC, and actinomycin; enzymes such as L-asparaginase; biological response modifiers such as interferon-alpha, IL-2, G-CSF and GM-CSF; miscellaneous agents including platinium coordination complexes such as cisplatin and carboplatin, 25 anthracenediones such as mitoxantrone, substituted urea such as hydroxyurea, methylhydrazine derivatives including N-methylhydrazine (MIH) and procarbazine, adrenocortical suppressants such as mitotane (o,p'-DDD) and aminoglutethimide; hormones and antagonists including adrenocorticosteroid antagonists such as prednisone and equivalents, dexamethasone and aminoglutethimide; progestin such as hydroxyprogesterone caproate, medroxyprogesterone acetate and megestrol acetate; estrogen such as diethylstilbestrol and ethinyl estradiol equivalents;. antiestrogen such as tamoxifen; androgens including testosterone propionate and fluoxymesterone/equivalents; antiandrogens such as flutamide, gonadotropin 5 releasing hormone analogs and leuprolide; and non-steroidal antiandrogens such as flutamide. Combination therapy with growth factors can include cytokines, lymphokines, growth factors, or other hematopoietic factors such as M-CSF, GM CSF, TNF, IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL 10 12, IL-13, 1L-14, 1L-15, 1L-16, IL-17, L-18, IFN, TNFO, TNF1, TNF2, G-CSF, Meg-CSF, GM-CSF, thrombopoietin, stem cell factor, and erythropoietin. Other are compositions can include known angiopoietins, for example Ang-1, -2, -4, -Y, and/or the human Ang-like polypeptide, and/or vascular endothelial growth factor (VEGF). Growth factors include angiogenin, bone morphogenic protein-1; bone 15 morphogenic protein-2, bone morphogenic protein-3, bone morphogenic protein 4, bone morphogenic protein-5, bone morphogenic protein-6, bone morphogenic protein-7, bone morphogenic protein-8, bone morphogenic protein-9, bone morphogenic protein-10, bone morphogenic protein-11, bone morphogenic protein-12, bone morphogenic protein-13, bone morphogenic protein-14, bone 20 morphogenic protein-15, bone morphogenic protein receptor IA, bone morphogenic protein receptor IB, brain derived neurotrophic factor, ciliary neutrophic factor, ciliary neutrophic factor receptor, cytokine-induced neutrophil chernotactic factor 1, cytokine-induced neutrophil, chemotactic factor 2, cytokine induced neutrophil chemotactic factor 2, endothelial cell growth factor, endothelin 25 1, epidermal growth factor, epithelial-derived neutrophil attractant, fibroblast growth factor 4, fibroblast growth factor 5, fibroblast growth factor 6, fibroblast growth factor 7, fibroblast growth factor 8, fibroblast growth factor 8b, fibroblast growth factor Sc, fibroblast growth factor 9, fibroblast growth factor 10, fibroblast growth factor acidic, fibroblast growth factor basic, glial cell line-derived 30 neutrophic factor receptor-1, glial cell line-derived neutrophic factor receptor-2, growth related protein, growth related protein-1, growth related protein-2, growth 102 related protein-3, heparin-binding epidermal growth factor, hepatocyte growth factor, hepatocyte growth factor receptor, insulin-like growth factor I, insulin-like growth factor receptor, insulin-like growth factor I, insulin-like growth factor binding protein, keratinocyte. growth factor, leukemia inhibitory factor, leukemia 5 inhibitory factor receptor-1, nerve growth factor nerve growth factor receptor, neurotrophin-3, neurotrophin-4, placenta growth factor, placenta growth factor 2, platelet-derived endothelial cell growth factor, platelet derived growth factor, platelet derived growth factor A chain, platelet derived growth factor AA, platelet derived growth factor AB, platelet derived growth factor B chain, platelet derived 10 growth factor BB, platelet derived growth factor receptor-1, platelet derived growth factor receptor-2, pre-B cell growth stimulating factor, stem cell factor, stem cell factor receptor, transforming growth factor-1, transforming growth factor-2, transforming growth factor-1, transforming growth factor-1.2, transforming growth factor-2,, transforming growth factor-3, transforming growth 15 factor-5, latent transforming growth factor-1, transforming growth factor-I binding protein I, transforming growth factor-i binding protein II, transforming growth factor-1. binding protein III, tumor necrosis factor receptor type I, tumor necrosis factor receptor type II, urokinase-type plasminogen activator receptor, vascular endothelial growth. factor, and chimeric proteins and biologically or 20 immunologically active fragments thereof. Immunotherapeutics Immunotherapeutics generally rely on the use of immune effector cells and molecules to target and destroy cancer cells. The immune effectors may be, for 25 example, a peptibody of the present invention that recognizes some marker on the surface of a target cell. The peptibody alone may serve as an effector of therapy or it may recruit other cells to actually effect cell killing. The peptibody may also be conjugated to a drug or toxin chemotherapeuticc, radionuclide, ricin A chain, cholera toxin, pertussis toxin, etc.) and thus may merely serve as a targeting agent. 30 According to the present invention, mutant forms of Ang-2 may be targeted by immunotherapy either peptibodies or peptibody conjugates of the 103 invention. It is particularly contemplated that the peptibody compositions of the invention may be used in a combined therapy approach in conjunction with Ang-2 targeted therapy. Passive immunotherapy has proved to be particularly effective against a 5 number of cancers. See, for example, WO 98/39027. The following examples are intended for illustration purposes only, and should not be construed as limiting the scope of the invention in any way. Example 1 10 Ang-2 Expression in Pathological and Normal Tissue Ang-2 expression was examined in normal and pathological tissue using in situ hybridization. Fragments of the human (Genbank Accession Number: AF004327, nucleotides 1274-1726) and murine (Genbank Accession Number: AF004326; nucleotides 1135-1588) Ang-2 sequences were amplified by reverse 15 transcriptase-PCR from human or murine fetal lung cDNA, cloned into the pGEM-T plasmid and verified by sequencing. 3 3 P-labeled antisense RNA probes were transcribed from linearized plasmid templates using 3 3 P -UTP and RNA polymerase. Blocks of formaldehyde-fixed, paraffin-embedded tissues were sectioned at 5 im and collected on charged slides. Prior to in situ hybridization, 20 tissues were permeabilized with 0.2M HCL, followed by digestion with Proteinase K, and acetylation with triethanolamine and acetic anhydride. Sections were hybridized with the radio labeled probe overnight at 55*C then subjected to RNase digestion and a high stringency wash in about 0.1 X SSC at 55*C. Slides were dipped in Kodak NTB2 emulsion, exposed at 4"C for 2-3 weeks, developed, 25 and counterstained. Sections were examined with dark field and standard illumination to allow simultaneous evaluation of tissue morphology and hybridization signal. The results indicated that in the normal postnatal human, Ang-2 expression is restricted to the few tissues containing angiogenic vasculature, such 30 as the ovary, placenta, and uterus. No Ang-2 expression was detectable in normal adult human heart, brain, kidney, liver, lung, pancreas, spleen, muscle, tonsil, 104 thymus, appendix, lymph node, gall bladder, prostate or testis. In five-week-old mouse (but not adult monkey or human), kidneys displayed prominent Ang-2 expression in the vasa recta. To determine whether this expression was a remnant of embryonic development, this experiment was repeated on kidneys derived from 5 mice ranging in age up to one-year-old using the murine Ang-2 probe and conditions described above. Ang-2 expression Was observed to decrease during neonatal development, but was still evident in kidneys of one-year-old mice. Ang-2 expression was also detected in virtually all tumor types tested, including, primary human tumors such as colon carcinoma (5 cases), breast 10 carcinoma (10 cases), lung carcinoma (8 cases), glioblastoma (1 case), metastatic human tumors such as breast carcinoma (2 cases), lung carcinoma (2 cases) and ovarian carcinoma (2 cases) which had metastized to brain, and rodent tumor models such as C6 (rat glioma), HT29 (human colon carcinoma), Colo-205 (human colon carcinoma), HCT1 16 (human colon carcinoma), A431 (human 15 epiderimoid carcinoma), A673 (human rhabdomyosarcoma), HT1080 (human fibrosarcoma), PC-3 (human prostate carcinoma), B16F1O (murine melanoma), MethA (murine sarcoma), and Lewis lung carcinoma mets. Additionally, Ang-2 expression Was detected in neovessels growing into a Matrigel plug in response to VEGF and in a mouse hypoxia model of retinopathy of prematurity. 20 Example 2 Molecular Assays to Evaluate Ang-2 Peptibodies Molecular assays (Affinity ELISA, Neutralization ELISA, and BlAcore) were developed to assess direct peptibody binding to Ang-2 and related family 25 members, and the effect of peptibodies on the Ang-2:Tie-2 interaction. These in vitro assays are described as follows. Affinity ELISA For the initial screening of candidate anti-Ang-2 peptibodies, purified 30 human Ang-2 (R&D Systems, Inc; catalog number 623-AN; Ang-2 is provided as a mixture of 2 truncated versions) or murine Ang-2 polypeptide (prepared as 105 described above) were used. For confirmatory binding assays, human Ang-2 was obtained from conditioned media of human 293T cells transfected with full length human Ang-2 DNA and cultured in serum free Dulbecco's Modified Eagle Medium (DMEM) containing about 50 micrograms per ml of bovine serum 5 albumin (BSA). Using microtiter plates, approximately 100 microliters per well of Ang-2 was added to each well and the plates were incubated about 2 hours, after which the plates were washed with phosphate buffered saline (PBS) containing about 0.1 percent Tween-20 four times. The wells were then blocked using about 250 10 microliters per well of about 5 percent BSA in PBS, and the plates were incubated at room temperature for about 2 hours. After incubation, excess blocking solution was discarded, and about 100 microliters of each candidate anti-Ang-2 peptibody was added to a well in a dilution series starting at a concentration of about 40 nanomolar and then serially diluting 4-fold in PBS containing about 1 percent 15 BSA. The plates were then incubated overnight at room temperature. After incubation, plates were washed with PBS containing about 0.1 percent Tween-20. Washing was repeated four additional times, after which about 100 microliters per well of goat anti-human IgG(Fc)-BRP (Pierce Chemical Co., catalog # 31416) previously diluted 1:5000 in PBS containing 1 percent BSA was added. Plated 20 were incubated approximately 1 hour at room temperature. Plates were then washed five times in PBS containing about 0.1 percent Tween-20, after which about 100 microliters per well of TMB (3,3',5,5'-Tetramethylbenzidine Liquid Substrate System; Sigma Chemical Company, St. Louis, MO, catalog number T8665) substrate was added and plates were incubated about 5-15 minutes until 25 blue color developed. Absorbance was then read in a spectrophotometer at about 370 nm. Neutralization ELISA Microtiter plates to which human Ang-2 polypeptide was bound were 30 prepared as described for the Affinity ELISA. Candidate anti-Ang-2 peptibodies were titrated from 1000nM to 0.2pM in 4-fold dilutions in a solution of PBS 106 containing about 1% BSA and about lnM Tie-2 (provided as a Tie-2-Fc molecule where the Tie-2 portion contains only the soluble extracellular portion of the molecule; R&D Systems, catalog number-313-TI). After about 100 microliters of the antibody/Tie-2 solution was added to each well, the plates were incubated 5 overnight at room temperature, and then washed five times in PBS containing about 0.1 percent Tween-20. After washing, about 100 microliters per well of anti-Tie-2 antibody (Pharmingen Inc., catalog # 557039) was added to a final concentration of about 1 microgram per ml, and the plates were incubated about 1 hour at room temperature. Next, about 100 microliters per well of goat anti 10 mouse-IgG-HRP (Pierce Chemical CO., catalog # 31432) was added at a dilution of 1:10,000 in PBS containing about I percent BSA. Plates were incubated at room temperature for about 1 hour, after which they were washed five times with PBS containing about 0.1 percent Tween-20. About 100 microliters per well of TMB substrate (described above) was then added and color was allowed to 15 develop. Absorbance was then read in a spectrophotomer at 370 nm. Affinity BIAcore An affinity analysis of each candidate Ang-2 peptibody was performed on a BIAcore*2000 (Biacore, Inc., Piscataway, NJ) with PBS and 0.005 percent P20 20 surfactant (Biacore, Inc.) as running buffer. Recombinant Protein.G (Repligen, Needham, MA) was immobilized to a research grade CM5 sensor chip (Biacore, Inc.) via primary amine groups using the Amine Coupling Kit (Biacore, Inc.) according to the manufacturer's suggested protocol. Binding assays were carried out by first capturing about 100 Ru of each 25 candidate anti-Ang-2 peptibody to the immobilized Protein G, after which various concentrations (0 - 100 nM) of huAng-2 or mAng-2 were injected over the bound antibody surface at a flow rate of 50 l1/min for 3 minutes. Peptibody binding kinetic parameters including ka (association rate constant), kd (dissociation rate constant) and KD (dissociation equilibrium constant) were determined using the 30 BIA evaluation 3.1 computer program (Biacore, Inc.). Lower dissociation equilibrium constants indicated greater affinity of the peptibody for Ang-2. 107 Example 3 Identification of Ang-2 Binding Peptides 5 1. Ana-2-Coated Magnetic Bead Preparation A: Ang-2 immobilization on magnetic beads For non-specific elution, the biotinylated Ang-2 protein (Biotinylated Recombinant Human Angiopoietin-2, R&D Systems, Inc.; catalog number BT 623) was immobilized on the Streptavidin Dynabeads (Dynal, Lake Success, NY) 10 at a concentration of about 4 pg of the biotinylated Ang-2 protein per 100 g1 of the bead stock from the manufacturer for all three rounds of selection. For antigen (Ang-2) and receptor (Tie-2) elutions, 2 pg of biotinylated Ang-2 protein was immobilized on 50 g1 of the Streptavidin Dynabeads for the second rounds of selection. The coating concentration was reduced to about 1 pg of biotinylated 15 Ang-2 protein per 50 pl of the bead stock for the third round of selection. By drawing the beads to one side of a tube using a magnet and pipetting away the liquid, the beads were washed five times with the phosphate buffer saline (PBS) and resuspended in PBS. The biotinylated Ang-2 protein was added to the washed beads at the above concentration and incubated with rotation for 1 hour at 20 room temperature, followed by a few hours to an overnight incubation at 4*C with rotation. Ang-2-coated beads were then blocked by adding BSA to about 1% final concentration and incubating overnight at 4 0 C with rotation. The resulting Ang-2 coated beads were then washed five times with PBS before being subjected to the selection procedures. 25 B. Negative selection bead preparation Additional beads were also prepared for negative selections. For each panning condition, 500 fl of the bead stock from the manufacturer was subjected to the above procedure (section 1A) except that the incubation step with 30 biotinylated Ang-2 was omitted. In the last washing step, the beads were divided into five 100 p1 aliquots. 108 2. Selection of Ang-2 Binding Phage A. Overall strategy Three filamentous phage libraries, designated as "TN8-IX" (5X10 9 5 independent transformants), "TN12-I" (1.4X10 9 independent transformants), and "Linear" (2.3X10 9 independent transformants) (all from Dyax Corp.), were used to select for Ang-2 binding phage. Each library was then subjected to either non specific elution, Ang-2 elution, and receptor elution (Tie-2). Nine different panning conditions were carried out for Ang-2 (TN8-IX using the non-specific 10 elution method, TN8-IX using the Ang-2 elution method, TN8-IX using the Tie-2 elution method, TN12-I using the non-specific elution method, TN12-I using the Ang-2 elution method, and TN12-I using.the Tie-2.elution.method, Linear using the non-specific elution method, Linear using the Ang-2 elution method, and Linear using the Tie-2 elution method). For all three libraries, the phage from the 15 first round of selection were eluted only in a non-specific manner for further rounds of selection. The Ang-2 and Tie-2 elutions were used in the second and third rounds of selection. For the Linear library, the-selection was carried to only the second round for the Ang-2 and Tie-2 elutions. 20 B. Negative selection For each panning condition, about 100 random library equivalents for. TN8-IX and TN12-I libraries (about 5 X 101 pfu for TN8-IX, and about.1.4 X. 1011 pfu for TN12-I) and about 10 random library equivalents for the linear library (about I X 1011 pfu) were aliquoted from the library stock and diluted to about 25 400 g1 with PBST (PBS with 0.05% Tween-20). After the last washing, liquid was drawn out from the first 100 R1 aliquot of the beads prepared for negative selection (section 1B), the approximately 400 p1 diluted library stock was added to the beads. The resulting mixture was incubated for about 10 minutes at room temperature with rotation. The phage supernatant was drawn out using the 30 magnet and added to the second 100 sd aliquot for another negative selection step. In this way, five negative selection steps were performed. 109 C. Selection using the Ang-2 protein coated beads The phage supernatant after the last negative selection step (section IB) was added to the Ang-2 coated beads (section 1A). This mixture was incubated 5 with rotation for one to two hours at room temperature, allowing phage to bind to the target protein. After the supernatant was discarded, the beads were washed about ten times with PBST followed by two washes with PBS. D. Non-specific elution 10 After the final washing liquid was drawn out (section 2C), about 1 ml of Min A salts solution (60 mM K 2

BPO

4 , 33 mM KH 2

PO

4 , 7.6 mM (NH 4 )S0 4 , and 1.7 mM sodium citrate) was added to the beads. This bead mixture was added directly to. a concentrated bacteria sample for infection (see below section 3A and 3B). 15 E. Antigen (Ang-2) elution of bound phage For round 2, after the last washing step (section 2C), the bound phage were eluted from the magnetic beads by adding 100 gl of 1 pM, 0.1 nM, and 10 nM recombinant Ang-2 protein (Recombinant Human Angiopoietin-2, R&D 20 Systems, Inc., Minneapolis, Minnesota) successively with a 3 0 -minute incubation for each condition. The remaining phage were eluted non-specifically (section 2D). The eluted phage from 10 nM and non-specific elutions were combined, and they were subjected to the third round of selection (see Section 4, below). For round 3, after the last washing step (section 2C), the bound phage 25 were eluted from the magnetic beads by adding about 1 nM recombinant Ang-2 protein, and 10 nM recombinant Ang-2 protein successively with a 30-minute incubation for each condition. In addition, the phage were eluted with I ml of 100 mM triethylamine solution (Sigma, St. Louis, Missouri) for about 10 minutes on a rotator. The pH of the phage-containing the triethylamine solution was 30 neutralized with 0.5 ml of 1 M Tris-HCl (pH 7.5). After the last elution with 100 110 mM triethylamine solution, the remaining phage were eluted by adding beads to the bacteria (section 2D). F. Receptor (Tie-2) elution of bound phage 5 For round 2, after the last washing step (section 2C), the bound phage were eluted from the magnetic beads by adding about 100 tl of 1 pM, 0.1 nM, and 10 nM recombinant Tie-2 protein (Recombinant Human Tie-2-Fc Chimera, R&D Systems, Inc., Minneapolis, Minnesota) successively with a 30-minute incubation for each condition. The remaining phage were eluted non-specifically 10 (section 2D). The eluted phage from 10 nM and non-specific elutions were combined and they were subjected to the third round of selection (see below section 4). For round 3, after the last washing step (section 2C), the bound phage were eluted from the magnetic beads by adding about 1 nM of recombinant Ang-2 15 protein, and 10 nM recombinant Tie-2 protein successively with a 30-minute incubation for each condition. In addition, the phage were eluted with 1 ml of.100 mM triethylamine solution (Sigma, St. Louis, Missouri) for 10 minutes on a rotator. The pH of the phage containing the triethylamine solution was neutralized with 0.5 ml of 1 M Tris-HCl (pH 7.5). After the last elution with 100 20 mM triethylamine solution, the remaining phage were eluted by adding beads to the bacteria (section 2D). 3. Amplification A. Preparation of plating cells 25 Fresh E. Coli. (XL-1 Blue MIR') culture was grown to an OD 60 0 of about 0.5 in LB media containing about 12.5 ptg/ml tetracycline. For each panning condition, about 20 ml of this culture was chilled on ice and centrifuged. The bacteria pellet was resuspended in about 1 ml of the Min A Salts solution. -30 B. Transduction 111 Each mixture from each different elution method set forth above (sections 2D, 2E and 2F) was added to a concentrated bacteria sample (section 3A) and incubated at about 37 *C for about 15 minutes. Approximately 2 ml of NZCYM media (2XNZCYM, 50 pg/ml Ampicillin) was added to each mixture and 5 incubated at about 37 *C for 15 minutes. The resulting 4 ml solution was plated on a large NZCYM agar plate containing about 50 gg/ml Ampicillin and incubated overnight at 37 *C. C. Phage Harvesting 10 Each bacteria/phage mixture was grown overnight on a large NZCYM agar plate (section 3B), after which they were scraped off into about 35 ml of LB media. The agar plate was further rinsed with additional 35 ml of LB media. The resulting bacteria/phage mixture in LB media was centrifuged to pellet the bacteria away. Approximately 50 ml of the phage supernatant was then 15 transferred to a fresh tube, and about 12.5 ml of PEG solution (20% PEG8000, 3.5M ammonium acetate) was added and incubated on ice for 2 hours to precipitate phage. The precipitated phage were centrifuged down and resuspended in 6 ml of the phage resuspension buffer (250 mM NaC1, 100 mM Tris pH8, I mM EDTA). This phage solution was further purified by centrifuging 20 away the remaining bacteria and precipitating the phage for the second time by adding about 1.5 ml of the PEG solution. After a centrifugation step, the phage pellet was resuspended in about 400 pl of PBS. This solution was subjected to a final centrifugation to rid the solution of any remaining bacterial debris. The resulting phage preparation was titered using standard plaque forming assays. 25 4. Additional Selection and Amplification In the second round, the amplified phage preparation (about 1010 pfu) from the first round (section 3C) was used as the input phage to perform the selection and amplification steps (sections 2 and 3). For the Ang-2 and Tie-2.elutions, 30 phage from 10 nM and non-specific elutions were combined and amplified for the 112 third round of selection. The amplified phage preparation (about 109 pfu) from the 2 "d round in turn was used as the input phage to perform 3 rd round of selection and amplification (sections 2 and 3). After the elution steps (sections 2D, 2E, and rd 2F) of the 3r round, a small fraction of the eluted phage was plated out as in the 5 plaque formation- assay (section 3C). Individual plaques were picked and placed into 96 well microtiter plates containing 100 [il of TE buffer in each well. These master plates were incubated at 4'C overnight to allow phage to elute into the TE buffer. 10 5. Clonal Analysis The-phage clones were analyzed by phage ELISA and DNA sequencing. The sequences were ranked based on the combined results from these two assays. A. Phage ELISA 15 An.XL-1 Blue MRF' culture was grown until OD 6 oo reached about 0.5. About thirty ji of this culture was aliquoted into each well of a 96-well microtiter plate. About 10 R1 of eluted phage (section 4) was added to each well and allowed to infect bacteria for about 15 minutes at room temperature. About 100 ptl of LB media containing approximately 12.5 jg/ml of tetracycline and 20 approximately 50 Vtg/ml of ampicillin were added to each well. The mi6rotiter plate was then incubated with shaking overnight at about 37 *C. The recombinant Ang-2 protein (about 1 jig/ml in PBS) was allowed to bind to the 96 well Maxisorp plates (NUJNC) overnight at about 4*C. As a control, the pure streptavidin was coated onto a separate Maxisorp plate at about 2 jg/ml in PBS. 25 On the following day, liquid in the protein coated Maxisorp plates was discarded, and each well was blocked with about 300 pl of 5% milk solution at about 4"C overnight (alternatively, 1 hour at room temperature). The milk solution was then discarded, and the wells were washed three times with the PBST solution. After the last washing step, about 50 41 of PBST-4% milk was 30 added to each well of the protein coated Maxisorp plates. About 50 g1 of 113 overnight cultures from each well in the 96 well microtiter plate was transferred to the corresponding wells of the Ang-2 coated plates as well as the control streptavidin coated plates. The 100 pl mixture in the each type of plate was incubated for bout I hour at room temperature. The liquid was discarded from the 5 Maxisorp plates, and the wells were washed about three times with PBST. The HRP-conjugated anti-M13 antibody (Amersham Pharmacia Biotech) was diluted to about 1:7,500, and about 100 pl of the diluted solution was added to each well of the Maxisorp plates for an approximately 1 hour incubation at room temperature. The liquid was again discarded and the wells were washed about 10 five times with PBST. About 100 p.l of TMB substrate (Sigma) was then added to { each well, and the reaction was stopped with about 50 g1 of the 5N H- 2 S0 4 solution. The OD 450 was read on a spectrophotometer (Molecular Devices). B. Sequencing of the phage clones 15 For each phage clone, the sequencing template was prepared using PCR. The following oligonucleotide pair was used to amplify an approximately 500 nucleotide fragment: Primer 1: 5'-CGGCGCAACTATCGGTATCAAGCTG-3' (SEQ ID 20 NO: 54) Primer 2: 5'-CATGTACCGTAACACTGAGTTTCGTC-3' (SEQ ID NO:55) 25 The following mixture was prepared for each clone: Reagents Volume (p) / Tube dH 2 O 26.25 50% glycerol 10 1OX PCR Buffer (w/o MgCl 2 ) 5 25 mM MgCl 2 4 10 mM dNTP mix 1 114 100 pM rimer 1 0.25 100 pM rimer 2 0.25 Ta olymerase 0.25 . Phage in TE (section 4) 3 Final reaction volume 50 For PCR, a thermocycler (GeneAmp PCR System 9700, Applied Biosystems) was used to run the following program: 94*C for 5 minutes; (94*C for 30 sec, 55'C for 30 sec, 72C 'for 45 sec.) x 30 cycles; 72'C for 7 minutes; 5 cool to 4*C. The PCR product from each reaction was purified using the QIAquick Multiwell PCR Purification kit (Qiagen), following the manufacturer's protocol. Purified PCR product was then assayed by running about 10 pl of each PCR reaction mix with about 1 pl of dye (10 X BBXS agarose gel loading dye) on a 1% agarose gel. The remaining product was then sequenced using the ABI 377 10 Sequencer (Perkin Elmer) following the manufacturer recommended protocol. 6. Sequence ranking and consensus sequence determination A. Sequence ranking and analysis The peptide sequences that were translated from variable nucleotide 15 sequences (section 5B) were correlated to ELISA data. The clones that showed a high OD 45 0 in the Ang-2 coated wells and a low OD 450 in the streptavidin coated wells were given a higher priority ranking. The sequences that occurred multiple times were also given a high priority ranking. Candidate sequences were chosen based on these criteria for further analysis as peptides or peptibodies. 20 B. Consensus sequence determination Three different classes of consensus motifs were generated from the TN8-IX library as follows: KRPCEEXWGGCXYX (SEQ IDNO:56) 25 KRPCEEXFGGCXYX (SEQIIDNO:57) 115 XXXCXDXYWYCXXX (SEQIDNO:61) XXXCXDXYTY CXXX (SEQIDNO:62) XXXCXDXFWYCXXX (SEQIDNO:63) XXXCXDXFTY CXXX (SEQ ID NO:64) 5 XXXCXWDPWTCEXM (SEQIDNO:58) One consensus motif was generated from the TN12-I library: WSXCAWFXGXXXXXCRRX (SEQIDNO:59) 10 For all consensus motif sequences, the underlined "core amino acid sequences" from each consensus sequence were obtained by determining the most frequently occurring amino acid in each position. "X" refers to any naturally occurring amino acid. The two cysteines adjacent to the core sequences were 15 fixed amino acids in the TN8-IX and TN12-I libraries. The peptides identified as binding to Ang-2 are set forth in Table 3 below. Table 3: Ang-2 Binding Peptides Peptide Seq Id No. Sequence TNS-8 1 KRPCEEMWGGCNYD TN8-14 2 HQICKWDPWTCKHW TN8-Conl 3 KRPCEEIFGGCTYQ TN8-Con4 4 QEECEWDPWTCEHM TN12-9 5 FDYCEGVEDPFTFGCDNH L1 6 KFNPLDELEETLYEQFTFQQ C17 7 QYGCDGFLYGCMIN 20 Example 4 116 Construction of DNA Encoding Peptibodies The modified peptides selected as potentially. inhibitory to Ang-2:Tie-2 binding (see Table 3) were used to construct fusion proteins in which either a monomer of each peptide or a tandemdimer of each peptide (with a linker 5 between the monomer units) was fused in-frame to DNA encoding a linker followed by the Fc region of human IgG1. Each modified peptide was constructed by annealing pairs of oligonucleotides ("oligos") to generate a polynucleotide duplex encoding the peptide together with a linker comprised, depending on the peptide, of either five glycine residues, eight glycine residues or 10 one lysine residue; these constructs were generates as NdeI to XhoI fragments. These duplex polynucleotide molecules were ligated into the vector (pAMG21-Fc N-terminal, described further below) containing the human Fc gene, which had been previously digested with NdeI and XhoI. The resulting ligation mixtures were transformed by electroporation into E. coli strain 2596 cells (GM221, 15 described further below) using standard procedures. Clones were screened for the ability to produce the recombinant protein product and to possess the gene fusion having a correct nucleotide sequence. A single such clone was selected for each of the modified peptides (i.e., Fc-peptide fusion products). 20 Construction of pAMG21-Fc N-Terminal Vector pAMG21 Expression plasmid pAMG21 (ATCC No. 98113) is derived from expression vector pCFM1656 (ATCC No. 69576) and the expression vector system described in United States Patent No. 4,710,473, by following the 25 procedure described in published International Patent Application WO 00/24782 (see the portion of Example 2 therein extending from pages 100-103, as well as Figures 17A and 17B). 30 Fc N-terminal Vector 117 The Fc N-terminal vector was created using E. coli strain 3788, pAMG21 TpoGly5_Fc monomer; as a template. Information on the cloning of this strain can be found in WO 00/24782 (See Example 2 and Figure 10 therein). A 5' PCR primer (described further below) was designed to remove the Tpo peptide 5 sequence in pAMG Tpo Gly5 and replace it with a polylinker containing ApaLI and XhoI sites. Using strain 3788 as a template, PCR was performed with Expand Long Polymerase, using the oligonucleotide of SEQ ID NO: 8, below, as the 5' primer and a universal 3' primer, SEQ ID NO: 9, below. The resulting PCR product was gel purified and digested with restriction enzymes NdeI and BsrGI. 10 Both the plasmid and the polynucleotide encoding the peptide of interest together with its linker were gel purified using Qiagen (Chatsworth, CA) gel purification spin columns. The plasmid and insert were then ligated using standard ligation procedures, and the resulting ligation mixture was transformed into E. coli cells (strain 2596). Single clones were selected and DNA sequencing was performed. 15 A correct clone was identified and this was used as a vector source for the modified peptides described herein. 5'Primer: 20 ACAAACAAACATATGGGTGCACAGAAAGCGGCCGCAAAAAAA CTCGAGGGTGGAGGCGGTGGGGACA (SEQ ID NO: 8) 3' Primer: 25 GGTCATTACTGGACCGGATC (SEQ ID NO: 9) In addition to making these modified peptides as N-terminal fusions to Fc (N-terminal peptibodies), some of them were also made as C-terminal fusion products (C-terinal peptibodies). The vector used for making the C-terminal 30 fusions is described below. Construction of Fc C-terminal vector 35 118 The Fc C-terminal vector for modified peptides was created using E. coli strain 3728, pAMG21 FcGly5_ Tpo monomer, as a template. Information on the cloning of this strain can be found in WO 00/24782 (See Example 2 and Figure 7 therein). A 3' PCR primer (SEQ ID NO: 10) was designed to remove the Tpo 5 peptide sequence and to replace it with a polylinker containing ApaLI and XhoI sites. Using strain 3728 as a template, PCR was performed with Expand Long Polymerase using a universal 5' primer (SEQ ID NO: 11) and the aforementioned 3' primer. The resulting PCR product was gel purified and digested with restriction enzymes BsrGI and BamH[. Both the plasmid and the polynucleotide 10 encoding each peptides of interest with its linker were gel purified via Qiagen gel purification spin columns. The plasmid and insert were then ligated using standard ligation procedures, and the resulting ligation mixture was transformed into E. coli (strain 2596) cells. Single clones were selected and DNA sequencing was performed. A correct clone was identified and used as a source of vector for 15 modified peptides described herein. 5' Primer: CGTACAGGTTTACGCAAGAAAATGG (SEQ ID NO: 10) 20 3' Primer: TTTGTIGGATCCATTACTCGAGTTTT GCGGCCGCTTTCTGTG CACCACCACCTCCACCTITAC (SEQ ID NO: 11) 25 GM221 (#2596). Host strain #2596, used for expressing Fc-peptide fusion proteins, is an E. coli K-12 strain modified to contain the lux promoter, and both the temperature sensitive lambda repressor cI857s7 in the early ebg region and the lacI repressor in the late ebg region. The presence of these two repressor genes 30 allows the use of this host with a variety of expression systems The ATCC designation for this strain is 202174. Example 5 Production of Peptibodies 119 Expression in E. coli. Cultures of each of the pAMG21-Fc fusion constructs in E. coli GM221 were grown at 37*C in Terrific Broth medium (See Tartof and Hobbs, "Improved media for growing plasmid and cosmid clones", Bethesda Research Labs Focus, Volume 9, page 12, 1987, cited in aforementioned 5 Sambrook et al. reference). Induction of gene product expression from the luxPR promoter was achieved following the addition of the synthetic autoinducer, N-(3 oxohexanoyl)-DL-homoserine lactone, to the culture medium to a final concentration of 20 nanograms per milliliter (ng/ml). Cultures were incubated at 37*C for an additional six hours. The bacterial cultures were then examined by 10 microscopy for the presence of inclusion bodies and collected by centrifugation. Refractile inclusion bodies were observed in induced cultures, indicating that the Fc-fusions were most likely produced in the insoluble fraction in E. coli. Cell pellets were lysed directly by resuspension in Laemmli sample buffer containing 10% D-mercaptoethanol and then analyzed by SDS-PAGE. In most cases, an 15 intense coomassie-stained band of the appropriate molecular weight was observed on an SDS-PAGE gel. Purification. Cells were broken in water (1/10) using high pressure homogenization (two passes at 14,000 PSI), and inclusion bodies were harvested by centrifugation (4000 RPM in a J-6B centrifuge, for one hour). Inclusion 20 bodies were solubilized in 6 M guanidine, 50 mM Tris, 10 mM DTT, pH 8.5, for one hour at a 1/10 ratio. For linear peptides fused to Fc, the solubilized mixture was diluted twenty-five times into 2 M urea, 50 mM Tris, 160 mM arginine, 2 mM cysteine, pH 8.5. The oxidation was allowed to proceed for two days at 4 0 C, allowing formation of the disulfide-linked compound (i.e., Fc-peptide homdimer). 25 For cyclic peptides fused to Fc, this same protocol was followed with the addition of the following three folding conditions: (1) 2 M urea, 50 mM Tris, 160 mM arginine, 4 mM cysteine, 1mM cystamine, pH 8.5; (2) 4 M urea, 20% glycerol, 50 mM Tris, 160 mM arginine, 2 mM cysteine, pH 8.5; and (3) 4 M urea, 20% glycerol, 50 mM Tris, 160 mM arginine, 4 mM cysteine, 1mM cystamine, pH 8.5. 30 The refolded protein was dialyzed against 1.5 M urea, 50mM NaCl, 50mM Tris, pH 9.0. The pH of this mixture was lowered to pH 5 with acetic acid. The 120 precipitate was removed by centrifugation, and the supernatant was adjusted to a pH of from 5 to 6.5, depending on the isoelectric point of each fusion product. The protein was filtered and loaded at 4*C onto an SP-Sepharose HP column equilibrated in 20 mM NaAc, 50 mM NaCJ at the pH determined for each 5 construct. The protein was eluted using a 20-column volume linear gradient in the same buffer ranging from 50 mM NaCJ to 500 mM NaCl. The peak was pooled and filtered. The peptibodies generated using the procedures above are set forth in Table 4 below. 10 Table 4 Peptibody Peptibody Sequence Li (N) MGAQKFNPLDELEETLYEQFTFQQLEGGGGG-Fc (SEQ ID NO:12) Li (N) WT MKFNPLDELEETLYEQFIFQQLEGGGGG-Fc (SEQ ID NO:13) Li (N) 1K WT MKFNPLDELEETLYEQFTFQQGSGSATGGSGSTASSGS GSATHLEGGGGG-Fc (SEQ ID NO:14) 2xL1 (N) MGAQKFNPLDELEETLYEQFTFQQGGGGGGGGKFNPL DELEETLYEQFTFQQLEGGGGG-Fc (SEQ ID NO:15) 2xL1 (N) WT MKFNPLDELEETLYEQFTFQQGGGGGGGKFNPLDELEE TLYEQFTFQQLEGGGGG-Fc (SEQ ID NO: 16) Con4 (N) MGAQQEECEWDPWTCEHMLEGGGGG-Fc (SEQ ID NO:17) Con4 (N) 1K-WT MQEECEWDPWTCEHMGSGSATGGSGSTASSGSGSATH LEGGGGG-Fc (SEQ ID NO: 18) MGAQQEECEWDPWTCEHMGSGSATGGSGSTASSGSGS 2xCon4 (N) 1K ATHQEECEWDPWTCEHMLEGGGGG-Fc (SEQ ID NO:19) L1 (C) M-Fc-GGGGGAQKFNPLDELEETLYEQFTFQQLE (SEQ ID NO:20) 121 Li (C) 1K M-Fc GGGGGAQGSGSATGGSGSTASSGSGSATHKFNPLDELE ETLYEQFTFQQLE (SEQ ID NO:21) 2xL1 (C) M-Fc GGGGGAQKFNPLDELEETLYEQFTFQQGGGGGGGGKF NPLDELEETLY'EQFTFQQLE (SEQ IID NO:22) M-Fc-GGGGGAQQEECEWDPWTCEHMLE (SEQ ID Con4 (C) NO:23) Con4 (C) 1K M-Fc GGGGGAQGSGSATGGSGSTASSGSGSATHQEECEWDP WTCEHMLE (SEQ ID NO:24) M-Fc 2xCon4 (C) 1K GGGGGAQQEECEWDPWTCEHMGSGSATGGSGSTASS GSGSATHQEECEWDPWTCEHMLE (SEQ ID NO:25) Con4-L1 (N) MGAQEECEWDPWTCEHMGGGGGGGGKFNPLDELEET

LYEQFTFQQGSGSATGGSGSTASSGSGSATHLEGGGGG

Fc (SEQ ID NO:26) M-Fc Con4-L1 (C) GGGGGAQGSGSATGGSGSTASSGSGSATHKFNPLDELE ETLYEQFTFQQGGGGGQEECEWDPWTCEHMLE (SEQ ID NO:27) TN-12-9 (N) MGAQ-FDYCEGVEDPFTFGCDNHLE-GGGGG-Fc (SEQ ID NO:28) MGAQ-QYGCDGFLYGCMINLE-GGGGG-Fc (SEQ ID C17 (N) NO:29) MGAQ-KRPCEEMWGGCNYDLEGGGGG-Fc (SEQ ID TN8-8 (N) NO:30) MGAQ-HQICKWDPWTCKHWLEGGGGG-Fc (SEQ ID TN8-14 (N) NO:31) MGAQ-KRPCEEIFGGCTYQLEGGGGG-Fc (SEQ ID ConI (N) NO:32) In Table 4, "Fc" refers to the human Fc IgGI sequence. Column two sets forth the amino acid sequence of the peptibody. The Fc portion thereof is labeled 5 "Fc", and is as set forth in SEQ ID NO: 60 below. It will be appreciated that where a label is used, for example, "Con4" or "Con-4", this refers to the Con-4 peptide, whereas use of the suffix "C", "(C)", or "-C"; or "N", "(N)", or "-N" 122 thereon indicates that the molecule is a peptibody as described herein. The suffixes "N", "(N)", or "-N" in a peptibody name indicate that the Ang-2-binding peptide (or peptides) is/are N-terminal to the Fc- domain, and the suffixes "C", "(C)" or "-C" indicate that the Ang-2-binding peptide (or peptides) is/are C 5 terminal to the Fc domain. Furthermore, 2xCon4 (C) 1K, as defined in SEQ ID NO: 25, may also be referred to without the "1K" suffix herein. The amino acid sequence of the Fc portion of each peptibody is as follows (from amino terminus to carboxyl terminus): 10 DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLISRTPEVTCVVVDV SHEDPEVKFNWY VDGVEVHNAKTKPREEQYNSTYRVVSVLTVL HQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPS RDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVL 15 DSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALINHYTQKSLS LSPGK (SEQ ID NO: 60) The DNA sequence (SEQ ID Nos: 33-53) encoding peptibodies corresponding to peptibody SEQ ID NOs: 12-32, respectively, in Table 4) is set 20 forth below: 123 SEQ ID NO: 33 ATGGGTGCACAGAAATTCAACCCGCTGGACGAACTGGAAGAAACTCT GTACGAACAGTTCACTTTCCAGCAGCTCGAGGGTGGAGGCGGTGGGG ACAAAACTCACACATGTCCACCTTGCCCAGCACCTGAACTCCTGGGGG 5 GACCGTCAGTTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGAT CTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGA

AGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGC

ATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTAC CGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGC 10 AAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATC GAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGT GTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG CCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGA . GTGOGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTC 15 CCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGT GGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGAT GCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTC TCCGGGTAAATAATGGATCC 20 SEQ ID NO:34 ATGAAATTCAACCCGCTGGACGAACTGGAAGAAACTCTGTACGAACA GTTCACTTTCCAGCAGCTCGAGGGTGGAGGCGGTGGGGACAAAACTCA CACATGTCCACCTTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGT TTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACC 25 CCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGA GGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAA GACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCA GCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACA AGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCA 30 TCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTG CCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGC CTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGC AATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGA CTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG 35 CAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGC TCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAA ATAA 124 SEQ ID NO:35 ATGAAATTCAACCCGCTGGACGAACTGGAAGAAACTCTGTACGAACA GTTCACTTTCCAGCAGGGATCCGGTTCTGCTACTGGTGGTTCCGGCTCC 5 ACCGCAAGCTCTGGTTCAGGCAGTGCGACTCATCTCGAGGGTGGAGGC GGTGGGGACAAAACTCACACATGTCCACCTTGCCCAGCACCTGAACTC CTGGGGGGACCGTCAGYTCCTCTTCCCCCCAAAACCCAAGGACACC CTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG AGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGT 10 GGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACA GCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGC TGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCA GCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGA ACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAA 15. CCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACAT CGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGA CCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAA GCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCAT GCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCC 20 TCTCCCTGTCTCCGGTAAATAA SEQ ID NO:36 ATGGGTGCACAGAAATTCAACCCGCTGGACGAACTGGAAGAAACTCT GTACGAACAGTTCACTTTCCAGCAGGGTGGTGGTGGTGGTGGCGGTGG 25 TAAGTTCAACCCACTGGATGAGCTGGAAGAGACTCTGTATGAACAGTT CACTTTCCAGCAACTCGAGGGTGGAGGCGGTGGGGACAAAACTCACA CATGTCCACCTTGCCCAGCACCTGAACTCCTGGGGGACCGTCAGTTT TCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCC CTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAG 30 GTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAG ACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAG CGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAA GTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCAT CTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGC 35 CCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCC TGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCA ATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGAC TCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGC AGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCT 40 CTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAA TAA. 125 SEQ ID NO:37 ATGAAATTCAACCCGCTGGACGAACTGGAAGAAACTCTGTACGAACA GTTCACTTTCCAGCAGGGTGGTGGTGGTGGCGGTGGTAAGTTCAACCC 5 ACTGGATGAGCTGGAAGAGACTCTGTATGAACAGTTCACTTTCCAGCA ACTCGAGGGTGGAGGCGGTGGGGACAAAACTOACACATGTCCACCTT GCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTTTCCTCTTCCCCCC AAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATG CGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTG 10 GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGG AGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCC TGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCC AACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAA AGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGG 15 ATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCT -TCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCG GAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCC TTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAG GGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCAC 20 TACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATAA SEQ ID NO:38 ATGGGTGCACAGCAGGAAGAATGCGAATGGGACCCATGGACTTGCGA ACACATGCTCGAGGGTGGAGGCGGTGGGGACAAAACTCACACATGTC 25 CACCTTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTTCCTCTT CCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGT CACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTT CAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGC CGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTC 30 ACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAA GGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAA AGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCAT CCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCA AAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGG 35 CAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGAC GGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGG CAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCAC AACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATAA 126 SEO ID NO:39 ATGCAGGAAGAATGCGAATGGGACCCATGGACTTGCGAACACATGGG ATCCGGTTCTGCTACTGGTGGTTCCGGCTCCACCGCAAGCTCTGGTTCA 5 GGCAGTGCGACTCATCTCGAGGGTGGAGGCGGTGGGGACAAAACTCA CACATGTCCACCTTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGT TTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACC CCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGA GGTCAAGTrCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAA 10 GACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCA GCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACA AGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCA TCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTG CCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGC 15 CTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGC AATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGA CTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG CAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGC TCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAA 20 ATAA SEQ ID NO:40 ATGGGTGCACAGCAGGAAGAATGCGAATGGGACCCATGGACTTGCGA ACACATGGGATCCGGTTCTGCTACTGGTGGTTCCGGCTCCACCGCAAG 25 CTCTGGTTCAGGCAGTGCGACTCATCAGGAAGAATGCGAATGGGACCC ATGGACTTGCGAACACATGCTCGAGGGTGGAGGCGGTGGGGACAAAA CTCACACATGTCCACCTTGCCCAGCACCTGAACTCCTGGGGGGACCGT CAGTTTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCG GACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACC 30 CTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAQGTGCATAATG CCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTG GTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAG TACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAA ACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACAC 35 CCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGAC CTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGA GAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGC TGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACA AGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATG 40 AGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGG GTAAATAA 127 SEQ ID NO:41 ATGGACAAAACTCACACATGTCCACCTTGCCCAGCACCTGAACTCCTG GGGGGACCGTCAGTTTTCCTCTTCCCCCCAAAACCCAAGGACACCCTC 5 ATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGC CACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGA GGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCA CGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGA ATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCC 10 CCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACC ACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCA GGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGC CGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCA CGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCT 15 CACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTC CGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTC CCTGTCTCCGGGTAAAGGTGGAGGTGGTGGTGCACAGAAATTCAACCC GCTGGACGAGCTGGAAGAGACTCTGTACGAACAGTTTACTTTTCAACA GCTCGAGTAA 20 SEQ IDNO:42 ATGGACAAAACTCACACATGTCCACCTTGCCCAGCACCTGAACTCCTG GGGGGACCGTCAGTTTTCCTCTTCCCCCCAAAACCCAAGGACACCCTC ATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGC 25 CACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGA GGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCA CGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGA ATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCC CCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACC 30 ACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCA GGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGC CGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCA CGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCT CACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTC 35 CGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTC CCTGTCTCCGGGTAAAGGTGGAGGTGGTGGTGCACAGGGATCCGGTTC TGCTACTGGTGGTTCCGGCTCCACCGCAAGCTCTGGTTCAGGCAGTGC GACTCATAAATTCAACCCGCTGGACGAACTGGAAGAAACTCTGTACGA ACAGTTCACTTCCAGCAACTCGAGTAA 40 128 SEQ ID NO:43 ATGGACAAAACTCACACATGTCCACCTTGCCCAGCACCTGAACTCCTG GGGGGACCGTCAGTTITCCTCTTCCCCCCAAAACCCAAGGACACCCTC 5 -ATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGC CACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGA GGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCA CGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGA ATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCC 10 CCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACC ACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCA GGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGC CGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCA CGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCT 15 CACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTC CGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTC CCTGTCTCCGGGTAAAGGTGGAGGTGGTGGTGCACAGAAATTCAACCC GCTGGACGAACTGGAAGAAACTCTGTACGAACAGTTCACTTTCCAGCA GGGTGGTGGTGGTGGTGGCGGTGGTAAGTTCAACCCACTGGATGAGCT 20 GGAAGAGACTCTGTATGAACAGTTCACTTTCCAGCAACTCGAGTAA SEQ ID NO:44 ATGGACAAAACTCACACATGTCCACCTTGCCCAGCACCTGAACTCCTG GGGGGACCGTCAGT CCTCTTCCCCCCAAAACCCAAGGACACCCTC 25 ATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGC CACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGA GGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCA CGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGA ATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCC 30 CCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACC ACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCA GGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGC CGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCA CGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCT 35 CACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTC CGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTC CCTGTCTCCGGGTAAAGGTGGAGGTGGTGGTGCACAGCAGGAAGAAT GCGAATGGGACCCATGGACTTGCGAACACATGCTCGAGTAA 129 SEQ ID NO:45 ATGGACAAAACTCACACATGTCCACCTTGCCCAGCACCTGAACTCCTG GGGGGACCGTCAG'TTCCTCTTCCCCCCAAAACCCAAGGACACCCTC 5 ATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGC CACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGA GGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCA CGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGA ATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCC 10 CCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACC ACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCA GGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGC CGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCA CGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTCCTCTACAGCAAGCT 15 CACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTC CGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTC CCTGTCTCCGGGTAAAGGTGGAGGTGGTGGTGCACAGGGATCCGGTTC TGCTACTGGTGGTTCCGGCTCCACCGCAAGCTCTGGTTCAGGCAGTGC GACTCATCAGGAAGAATGCGAATGGGACCCATGGACTTGCGAACACA 20 TGCTCGAGTAA SEQ ID NO:46 ATGGACAAAACTCACACATGTCCACCTTGCCCAGCACCTGAACTCCTG GGGGGACCGTCAGT TCCTCTTCCCCCCAAAACCCAAGGACACCCTC 25 ATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGC CACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGA GGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCA CGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGA ATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCC 30 CCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACC ACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCA GGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGC CGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCA CGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCT 35 CACCGTGGACAAGAGCAGGTGGCAGCAUGGGAACGTCTTCTCATGCTC CGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTC CCTGTCTCCGGGTAAAGGTGGAGGTGGTGGTGCACAGCAGGAAGAAT GCGAATGGGACCCATGGACTTGCGAACACATGGGATCCGGTTCTGCTA CTGGTGGTTCCGGCTCCACCGCAAGCTCTGGTTCAGGCAGCGCGACTC 40 ATCAGGAAGAATGCGAATGGGACCCATGGACTTGCGAACACATGCTC GAGTAA 130 SEQ ID NO:47 ATGGGTGCACAGGAAGAATGCGAATGGGACCCATGGACTTGCGAACA CATGGGTGGTGGTGGTGGTGGCGGTGGTAAATTCAACCCGCTGGACGA 5 ACTGGAAGAAACTCTGTACGAACAGTTCACTTTCCAGCAGGGATCCGG TTCTGCTACTOGTGGTTCCGGCTCCACCGCAAGCTCTGGTTCAGGCAGT GCGACTCATCTCGAGGGTGGAGGCGGTGGgGACAAAACTCACACATGT CCACCTTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTTCCTCT TCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGG 10 TCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGT TCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAG CCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTC ACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAA GGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAA 15 AGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCAT CCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCA AAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGG CAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGAC GGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGG 20 CAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCAC AACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATAA SEO ID NO:48 ATGGACAAAACTCACACATGTCCACCTTGCCCAGCACCTGAACTCCTG 25 GGGGGACCGTCAGTTTCCTCTTCCCCCCAAAACCCAAGGACACCCTC ATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGC CACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGA GGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCA CGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGA 30 ATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCC CCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACC ACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCA GGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGC CGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCA 35 CGCCTCCCGTGCTGACTCCGACGGCTCCTCTTCCTCTACAGCAAGCT . CACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTC CGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTC CCTGTCTCCGGGTAAAGGTGGAGGTGGTGGTGCACAGGGATCCGGTTC TGCTACTGGTGGTTCCGGCTCCACCGCAAGCTCTGGTTCAGGCAGTGC 40 GACTCATAAATTCAACCCGCTGGACGAACTGGAAGAAACTCTGTACGA ACAGTTCACTTTCCAGCAGGGTGGTGGCGGTGGTCAGGAAGAATGCGA ATGGGACCCATGGACTTGCGAACACATGCTCGAGTAA 131 SEQ ID NO:49 ATGGGTGCACAGTTCGACTACTGCGAAGGTGTTGAAGACCCGTTCACT TTCGGTTGCGACAACCACCTCGAGGGTGGAGGCGGTGGGGACAAAAC 5 TCACACATGTCCACCTTGCCCAGCACCTGAACTCCTGGGGGGACCGTC AGTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGG ACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCT GAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCC AAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGT 10 CAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTA CAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAAC CATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCC TGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCT GCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGA 15 GCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTG GACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAG AGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAG GCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGT AAATAA 20 SEO ID NO:50 ATGGGTGCACAGCAGTACGGTTGCGACGGrTTCTGTACGGTTGCATG ATCAACCTCGAGGGTGGAGGCGGTGGGGACAAAACTCACACATGTCC ACCTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAG1TTCCTCTTC 25 CCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTC ACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTC AACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCC GCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCA CCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAG 30 GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAA GCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCC CGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAA AGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGC AGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGAC 35 GGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGG CAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCAC AACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATAA 132 SEQ ID NO:51 ATGGGTGCACAGAAACGCCCATGCGAAGAAATGTGGGGTGGTTGCAA CTACGACCTCGAGGGTGGAGGCGGTGGGGACAAAACTCACACATGTC 5 CACCTTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTTTTCCTCTT CCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGT CACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTT CAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGC CGCGG'GAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTC 10 ACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAA GGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAA AGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCAT CCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCA AAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGG 15 CAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGAC GGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGG CAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCAC AACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATAA 20 SEQ ID NO:52 ATGGGTGCACAGCACCAGATCTGCAAATGGGACCCGTGGACCTGCAA ACACTGGCTCGAGGGTGGAGGCGGTGGGGACAAAACTCACACATGTC CACCTTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTTTTCCTCTT CCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGT 25 CACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTT CAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGC CGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTC ACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAA GGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAA 30 AGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCAT CCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCA AAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGG CAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGAC GGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGG 35 CAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCAC AACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATAA 133 SSEQ-ID NO:53 ATGGGTGCACAGAAACGTCCATGCGAAGAAATCTTCGGTGGTTGCACC TACCAGCTCGAGGGTGGAGGCGGTGGGGACAAAACTCACACATGTCC 5 ACCTTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTTITCCTCTTC CCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTC ACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTC AACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCC GCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCA 10 CCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAG GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAA GCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCC CGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAA AGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGC 15 AGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGAC GGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGG CAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCAC AACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATAA 20 Example 6 Peptibody Assays Fourteen of the peptibodies were tested using the neutralization ELISA, and three of the peptibodies were tested using the affinity ELISA. The results are 25 set forth in Table 5. Table 5 ShAng-2 mAng-2 hAn -1 IC50 EC50 EC50 Pe ptibody (nM) (nM) IC 50 (nM) (nM) IC 50 (nM) EC 50 (nM) 2xCon4 (C) 1K 0.04 0.02 . Con4-L1 (C) 0.05 - 0.04 Con4 (C) 0.20 0.30 2xL1 (N) 0.65 0.80 Con4 (N) 0.85 0.03 0.72 0.07 No Inhibition No Binding 2xL1 (C) 0.90 1.0 Con4 (N) 1K WT 1.9 L1 (N) 6 11 # No Inhibition C17 (N) 9 13 No Inhibition 12-9 (N) 21 7.7 No Inhibition 134 Con1 (N) 26 - 200 No Inhibition 8-14 (N) 45 _33 No Inhibition Li (C) 65 37 8-8 (N) 80 -700 No Inhibition Negative Control No No No Peptibody 4883 Inhibition Binding No Inhibition Binding No Inhibition No Binding The amino acid sequence of negative control peptibody 4883 is as follows (the Fc portion is underlined, the linker is "GGGGG"; and the peptide portion-is in bold): 5 MDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLHSRTPEVTCVVVD VSBEDPE VKFNWYVDGVEVNAKTKPREEOYNSTYRVVSVLTVL HODWLNGKEYKCKVSNKALPAPIEKTISKAKGOPR FPOVYTLPPS RDELTKNOVSLTCLVKGFYPSDIAVEWESNGOPENNYKTTPPVLD SDGSFFLYSKLTVDKSRWOOGNVFSCSVlYHATHNHYTOKSTSTS 10 PGK-GGGGG-CTAGYHWNSDCECCRRN (SEQ ID NO: 243) It will be appreciated that use of the term "No Inhibition" herein is not meant to indicate that the compounds have no inhibitory qualities. Rather, "No Inhibition" as used herein refers to those compounds which when tested using the neutralization ELISA assay under the conditions described herein exhibited an 15 IC 50 value of greater than 1000 nM, which was the highest concentration at which these compounds were screened. While significant inhibitory qualities were not observed for the molecules labeled as exhibiting "no inhibition", it will be appreciated that those molecules may in fact demonstrate inhibitory qualities under different assay conditions, or in different assays. In a preferred 20 embodiment, it will be appreciated that the invention relates to peptibodies that have inhibitory qualities using the assays described herein. Two of the peptibodies were tested using the affinity BIAcore assay (as described in Exarnple 2). The results are set forth in Table 6 below. 25 135 Table 6 Peptibodv (Pb) Affinities for hAng-2 and mAng-2 hAng-2 mAng-2 Peptibody KD ka(1/Ms) kd(1/s) KD (nM) ka(1/Ms) kd(1/s) (nM) Pb Li (N) 3.1 2.9 x 10 5 9.1 x 10 0.42 5.6 x 105 2.3 x 1-4 Con4 (N) 0.67 3.3 x 10 5 2.2 x 10-4 0.60 7.3 x 105 4.4 x 10-4 TN12-9 (N) 8.2 1.2 x 10 5 1.0 X 10- 0.32 7.2 x 1 2.3 x 10 5 Exaiple 7 Therapeutic Efficacy Studies With Systemically Administered Ang-2 Peptibody 10 Ang-2 peptibody, TN8-Con4-C, was administered subcutaneously to A431 tumor-bearing mice at a once-per-day schedule 72 hours after tumor challenge. The doses of peptibody used were 1000, 200, 40 and Sug/mouse/day. A total of 20 doses was given to all animals. Tumor volumes and body weights were 15 recorded three times/week. At the end of the study, animals were sacrificed, and their sera were collected for measuring peptibody levels by ELISA. Tumors and a panel of normal tissues were collected from all groups. The results are shown in Figure 1. As can be seen, significant differences in tumor growth were observed between the Ang-2 peptibody treated group and 20 vehicle control. All four doses of Ang-2 peptibody inhibited tumor growth as compared to vehicle controls (p <0.0001 vs. vehicle control using repeated measure ANOVA). In contrast, tumors in the control group continued to grow at a much greater rate. Treatment with this peptibody had no significant effect on terminal body weights, organ weights or hematology parameters of the animals 25 treated at the above doses. 136 Example 8 1. Construction Of Ang-2 Secondary Peptide Libraries A. Electrocompetent E.coli Cells Epicurian Coli® XL1-Blue MRF' electroporation competent cells 5 (Stratagene #200158) were purchased from Stratagene (Stratagene Cloning Systems, La Jolla, CA). B. Modification of pCES1 Vector PCR was performed using Extend Long Template PCR Systems (Roche 10 Diagnostics Corp., Indianapolis, IN) with 1 pg of pCES1 vector (TargetQuest Inc.) as a template. PCR mixture volume was 100 p1 which contained 1x PCR buffer, 200 nM of each of the two phmers: 5' CAAACGAATGGATCCTCATTAAAGCCAGA-3' (SEQ ID NO: 244) and 5'

GGTGGTGCGGCCGCACTCGAGACTGTTGAAAGTTGTTTAGCA-

3 ' (SEQ 15 ID NO: 245), 200 nM dNTP, and 3 units (U) of Tag DNA polymerase. The TRIO-Thermoblock (Biometra) PCR system was run as follows: 94*C for 5 minutes; 30 cycles of 94'C for 30 seconds, 50*C for 30 seconds, 72*C for 45 seconds; and 72*C for 10 minutes; cool to 4*C. The PCR products were then rnm on a 1% agarose gel and purified with 20 QIAGEN Spin Column (QIAGEN Inc., Valencia, CA) according to the manufacturer's protocols. A second PCR reaction was performed with 5 si of PCR products and 200 nM of each of the two primer 5' CAAACGAATGGATCCTCATTAAAGCCAGA-3' (SEQ ID NO: 246) and 5'

AACACAAAAGTGCACAGGGTGGAGGTGGTGGTGCGGCCGCACT-

3 ' 25 (SEQ ID NO: 247) under the same PCR conditions as described above. The PCR products and original pCES 1 vector were then digested separately in a 100 pl reaction containing 1x NEB2 buffer, 60 U of ApaLI (New England Biolabs, Beverly, MA), 60 U of BamII (New England Biolabs) at 37*C for 1 hour. The digested DNA was then purified using a.QIAGEN Spin Column 137 and ligated together in a 40 g1 reaction containing 1x ligation buffer and 40 U of T4 DNA ligase (New England Biolabs) at room temperature overnight. The vectors were transfected into E. coli and incubated at 37 0 C overnight. Isolated single colonies were selected and plasmid was then purified using a 5 QIAGEN Spin Column. The correct insert was confirmed by DNA sequencing. One microgram of modified pCES1 vector DNA (from section 1B above) was transformed into 40 il of electrocompetent XL1-blue E.coli (from section 1A 10 above) using the Gene Pulser I (BIO-RAD, Hercules, CA) set at 2500V, 25 pF, and 200 ohms. The transformed bacteria sample was then transferred immediately into a tube containing 960 91 of SOC (2% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KC], 20 MM glucose, 10 mM MgSO 4 , 10mM 1 MgCl 2 ), and the culture was allowed to grow at 37 0 C with shaking for 1 hour. 15 The cells were then spread onto the 2xYTAGT (2xYT with 100ug/mi ampicillin, 1 2 .5ug/lrp tetracycline and 2% glucose) agar plate and incubated at 37*C overnight. A single colony was confirmed by sequencing and used to inoculate 2 liters of 2xYTAGT media at 37"C with shaking overnight. The plasmid vector DNA was purified with QIAGEN Plasmid Maxi Kit according to 20 the manufacturer's protocols. D. Digestion of VecoDN Total about 2000 micrograms of vector DNA (from section IC above) .was digested in 5000 ptl reaction containing 1x NEB buffer2, 300 U of ApaLI, and 300 25 U of XhoI at 37*C overnight. The restriction digest reaction was incubated overnight at 37 0 C and analyzed in a pre-made 0.8% agarose gel (Embi Tec, San Diego, CA). The linearized vector DNA was then excised from the gel and extracted with QIAquick Gel Extraction Kit (QIAGEN Inc.) according to the manufacturer's directions. 30 138 E. Preparation of Library Oligonucleotides Six library oligonucleotides (1 fixed and 5 doped) were designed based on the sequences that derived from the results described above, The one fixed library oligonucleotides was: 5 5'-CACAGTGCACAGGGTNNKNNKNNKNNKNNKNNKS ARTGGGATCCGTGGASCNNKNNKNNKNNKNNKNNKNNKCATT CTCTCGAGATCA-3' (library number 20) (SEQ ID NO: 248); 10 and two of the 70% doped library oligonucleotides were as follows: 5'-CACAGTGCACAGGGTNNKNNKNNKaaKcgKccKNNKga KgaKatKttKggKggKNNKacKtaKcaKNNKNNKNNKCATTCTC' TCGAGATCA-3' (library number 27); (SEQ ID NO: 249); 15 5'-CACAGTGCACAGGGTNNKaaKttKaaKccKctKgaKgaKctKgaKga KacKctKtaKgaKcaKttKacKttKcaKcaKNNKCATTCTCTCGAGATCA 3' (library number 99); (SEQ ID NO: 250); Lower case letters represent a mixture of 70% of the indicated base and 20 10% of each of the other three nucleotides). The other three of the 91% doped library oligonucleotides were as follows: 5'-CACAGTGCACAGGGTNNKNNKNNKcaKgaKgaKTGCgaKtg KgaccKtglacKTGCgaKcaKatKNNKNNKNNKCATTCTCTCGAGA 25 TC A-3' (library number 94); (SEQ ID NO: 251); 5'-CACAGTGCACAGGGTNNKttKgaKtaKNNKgaKggKgtKgaKgaKcc KttKacKttKggKNNKgaKaaKcaKNNKCATTCTCTCGAGATCA-3' (library number 25); (SEQ ID NO: 252); 30 and 139 5'-CACAGTGCACAGGGTNNKaaKttKaaKccKctKgaKgaKetKgaaKgoa KacKctKtaKgaKcaKttlacKttKcaKcaKNNKCATTCTCTCGAGATCA 3' (library number 26); (SEQ ID NO: 253); 5 For the oligos above, those skilled in the art will appreciate that "N" indicates that each of the four nucleotides (A, T, C, and G) are equally represented during oligo synthesis, and "K" indicates that nucleotides G and T were equally represented during oligo synthesis. Lower case letters represent a mixture of 91% 10 of the indicated base and 3% of each of the other three nucleotides. Each of these oligonucleotides was used as templates in PCR. Expand High Fidelity PCR System kit (Roche Diagnostics Corp.) was used for the PCR reactions. Each library oligo was amplified in a ninety six well 50 pl PCR reaction which contained 1 nM of a library oligonucleotide, 1X PCR 15 buffer, 300 nM of each of the primers: 5'-CACAGTGCACAGGGT-3' (SEQ ID NO: 254); and 5'-TGATCTCGAGAGAATG-3', (SEQ ID NO: 255); 20 200 M dNTP, 1.5 mM MgCl 2 , and 350 U of the Expand polymerase. The thermocycler (GeneAmp PCR System 9700, Applied Biosystems) was used to run the following program: 94*C for 5 minutes; 25 cycles of (94*C for 30 seconds, 52.5*C for 60 seconds, 72*C for 30 seconds); 72*C for 10 minutes; cool to 4*C. The free nucleotides were then removed using the QlAquick PCR 25 Purification Kit (QIAGEN Inc. Cat#28104) according to the manufacturer's protocols. 30 F. Digestion of Library Oligonucleotides 140 For each library the PCR products (section 1E) were digested in a 1200 Ll reaction that contained 1x NEB buffer2, 750 U of ApaLI, and 750 U of XhoI at 37*C overnight. The digested DNA was separated on a pre-made 3% agarose gel (Embi Tec). The DNA band of interest from each reaction was cut from the gel -5-- and extracted with-COSTAR Spin-X centrifuge-tube filter0,.22 pLm cellulose acetate (Corning Inc., Cat# 8160). G. Ligtion of Vector with Library Oligonucleotides The 450 [Ll ligation reaction contained the linearized vector (section 1D) 10 and each digested library PCR product (section IF) at 1:5 molar ratio, lx NEB ligation buffer, and 20,000 U of the T4 DNA ligase at 16*C overnight. The ligated products were incubated at 65*C for 20 minutes to inactivate the T4 DNA ligase and further incubated with 100 U NotI at 37*C for 2 hours to minimize vector self-ligation. The ligated products were then purified by a standard 15 phenol/chloroform extraction (Molecular Cloning: A Laboratory Manual, Maniatis et al., 3 rd Edition, Cold Spring Harbor Laboratory Press, 2000) and resuspended in 120 gl of H 2 0. H. Electroporation Transformation 20 For each library, twelve electroporation reactions were performed. For each transformation, 10 I of the ligated vector DNA (section 1G) and 300 I1 of XL1-BLUE MRF' cells (section 1A) were mixed in a 0.2-cm cuvette (BIO-RAD). The resulting mixture was pulsed by the Gene Pulser II'setting at 2500 V, 25 uF, and 200 ohms. The transformed bacteria from the twelve electroporation 25 reactions were then combined and transferred into a flask containing 26 ml of SOC for incubation at 37*C for 1 hour. The cells were added to 450 ml 2xYTAG and grown at 37"C with shaking for 5 hours. The cells were centrifuged at 4000 rpm for 15 minutes at 4*C. The cell pellets were then resuspended in 12 ml of 15% glycerol/2xYT and stored at -80*C. This was the primary stock of the 30 libraries. Titers showed library sizes of 5.0x10 9 (library number 20), 3.3 xl10 141 (library number 94), 4.7x109 (library number 25), 5.Ox 10 9 (library number 26), 3.0'x10 9 (library number 27), and 4.2x10 9 (library number 99) independent transformants. 5 2. Amplification of the Libraries A: Making Secondary Stock of the Libraries From the primary library cell stock (from section 1H above), sufficient cells to cover a loX excess of each library size were used to inoculate 2xYTAGT (2YT with 100ug/ml ampicillin, 12.5ug/ml tetracycline and 2% glucose) media so 10 that the starting OD 6 00 was 0.1. The cultures were allowed to grow at 37*C with shaking for several hours until the OD 6 00 = 0.5. A one-tenth aliquot from each library was taken out and grown up in separate flasks for another two hours at 37*C. These sub-cultures were then centrifuged at 4000 rpm using a Beckman JA-14 rotor for 10 minutes at 4*C, and the bacteria pellets resuspended in 7.0 ml 15 (for each library) of 15% glycerol/2xYT for storage at -80*C. B. Phage Induction M13KO7 helper phage aliquots (Amersham Pharmacia Biotech) were added to the remaining bacteria cultures at OD 6 00 = 0.5 (from Section 2A above) 20 to the final concentration of 3 x 109 pfu/mrl. The helper phage were allowed to infect bacteria at 37*C for 30 minutes without shaking and 30 minutes with slow shaking. The infected cells were centrifuged with 5000 rpm for 15 minutes at 4*C. The cell pellets were resuspended in the same volume (from section 2A above) with the 2xYTAK media (2YT with 100ug/ml ampicillin and 4 0ug/ml 25 kanamycin). The phagemid production was allowed to occur at 30*C overnight while shaking. 30 C. Harvest of Phage 142 The bacteria cultures from section 2B above were centrifuged at 5000 rpm for 15 minutes at 4 0 C. The supernatants were then transferred into new bottles, and 0.2 volume of 20% PEG/2.5M NaCl were added and incubated-on ice for 1 hour to precipitate the phagemids. Precipitated phagemids were centrifuged at 5 10,000 rpm for 30 minutes at 4*C and carefully resuspended with 100 ml of cold PBS. The phagemid solution was further purified by centrifuging away the remaining cells with 4000 rpm for 10 minutes at 4*C and precipitating the phagemids by adding 0.2 volume of 20% PEG/2.5M.NaCl. The phagemids Were centrifuged at 10,000 rpm for 30 minutes at 4*C, and the phagemid pellets 10 resuspended with 18 ml of cold PBS. Six ml of 60% glycerol solution was added to the' phagemid solution for storage at -80*C. The phakemid titers were determined by a standard procedure (Molecular Cloning, Maniatis et al 3 rd Edition). 15 3. Selection of Ang-2 Binding Phage A. Immobilization of Ang-2 on Magnetic Beads The biotinylated Ang-2 (from section 3A above) was immobilized on the Dynabead M-280 Streptavidin (DYNAL, Lake Success, NY) at a concentration of 2000 ng Ang-2 protein per 100 p1 of the bead stock from the manufacturer. After 20 drawing the beads to one side of a tube using a magnet and pipetting away the liquid, the beads were washed twice with phosphate buffer saline (PBS) and resuspended in PBS. The biotinylated Ang-2 protein was added to the washed beads at the above concentration and incubated with rotation for 1 hour at room temperature. The Ang-2 coated beads were then blocked by adding BSA to 2% 25 final concentration and incubating overnight at 4*C with rotation. The resulting Ang-2 coated beads were then washed twice with PBST (PBS with 0.05% Tween 20) before being subjected to the selection procedures. 30 B. Selection Using the An-2 Coated Beads 143 About 1000-fold library equivalent phagemids (from section 2C above) were blocked for one hour with 1 ml of PBS containing 2% BSA. The blocked phagemid sample was subjected to three negative selection steps by adding it to blank beads (same beads as section 3A but with no Ang-2 protein coating), and 5 this mixture was incubated at room temperature for 15 minutes with rotation. The phagemid containing supernatant was drawn out using magnet and transferred to a second tube containing blank beads (the same beads as described in section 3A above but without Ang-2 protein coated thereon), and this mixture incubated at room temperature for 15 minutes with rotation. 10 -The procedure was repeated. The phagernid containing supernatant was then drawn out using magnet and transferred to a new tube containing Ang-2 protein coated beads (from section 3A), and the mixture was incubated at room temperature for 1 hour with rotation. After the supernatant was discarded, the phagemid-bound-beads were washed 10 times with 2%milk-PBS; 10 times with 15 2% BSA-PBS; 10 times with PBST and twice with PBS. The phagemids were then allowed to elute in I ml of 100 mM triethylamine solution (Sigma, St. Louis, MO) for 10 minutes on a rotator. The pH of the phagemid containing solution was neutralized by adding 0.5 ml of 1 M Tris-HCl (pH 7.5). The resulting phagemids were used to infect 10 ml of freshly grown XL1-Blue MiRF' bacteria 20 (OD 600 about 0.5) at 37*C for 30 minutes without shaking and 30 minutes with slow shaking. All of the infected XL1-BLUE MIRF' cells were then plated on a 15X15cm 2xYTAG plate and incubated at 30*C overnight. C. Induction and Harvesting of Phage 25 A 10 ml aliquot of 2xYTAGT media was added to the plate (from section 3B) to resuspend XL1-BLUE MRF' cells. All XL1-BLUE MR' cells were collected in a tube, and a 250 ptl aliquot of these cells was added to 25 ml of 2xYTAGT and grown at 37'C until OD 6 00 = 0.5. The M13KO7 helper phage were added to a final concentration of 3 x 109 cfu/ml and incubated at 37*C for 30 30 minutes without shaking and 30 minutes with slow shaking. The cells were centrifuged with 5000 rpm for 10 minute at 4 0 C and resuspended with 25 ml of 144 2xYTAK. These bacteria were allowed to grow at 30"C overnight with shaking. The induced phagemids were harvest and purified as in section 2C. D. Second Round Selection 5 The second round selection was performed as outlined in section 3B to 3C except for the following. About 100-fold library equivalent phagemids resulting from section 3C was used as the input phagenid. The amount of biotinylated Ang-2 protein (section 3A) coat onto the Dynabead M-280-Streptavidin was decreased to 20 ng. The phage-bound-beads were then washed 10 times with 10 2%milk-PBS; 10 times with 2%BSA-PBS; 10 times with PBST, where the final wash involved 60 minutes incubation at room temperature in PBST. The beads were washed twice with PBS. The elution conditions were same as the first round (section 3B). 15 E. Third Round Selection The third round selection was performed as outlined in section 3B to 3C above except the following. About 10 fold library equivalent phagemids resulting from section 3D was used as the input phagemid. About 2ng of biotinylated Ang 2 protein (from section 3A) was used to coat onto the Dynabead M-280 20 Streptavidin. The phage-bound-beads were washed 10 times with 2%milk-PBS; 10 times with 2% BSA-PBS; 10 times with PBST, where the final wash involved 60 minutes incubation at room temperature in PBST. The beads were washed twice with PBS. The elution conditions were same as the first round (section 3B). 25 F. Fourth Round Selection The fourth round selection was performed as outlined in section 3B to 3C above except for the following. Library equivalent phagemids resulting from section 3E were used as the input phagemid. The amount of biotinylated Ang-2 protein (section 3A) coat onto the Dynabead M-280 Streptavidin was decreased to 30 0.4 ng for libraries 25, 26, and 27. For libraries 20 and 94, the coating amount was kept as the third round at 2 ng. The library 99 was not carried to the fourth 145 round selection step. The elution conditions were same as the first round (section 3B). 4. CLONAL ANALYSIS 5 A. Preparation of Master Plate Single colonies from the second round selection were picked and inoculated into 96 well plates containing 120 pl of 2xYTAGT per well. The 96 well plates were incubated in 30*C shaker for overnight. Forty microliters of 60% glycerol were added per well for storage at -80*C. 10 B. Phagemid ELISA About 2 p.1 aliquots of cells from the master plate (from section 4A above) were inoculated into a fresh Costar@ 96 well plate (Corning incorporated, Corning, NY, cat. #9794) which contained 100 [d of 2xYTAGT per well, and this 15 new plate of cells was grown at 37*C until approximate

OD

60 0 = 0.5. Forty p of 2xYTAGT containing M13KO7 helper phage (1.5 x 1013 cfu/ml) was added to each well, and the 96 well plate was incubated at 37*C for 30 minutes without shaking and another 30 minutes with slow shaking. The plate was centrifuged at 2000 rpm (Beckman CS-6R tabletop centrifuge) for 10 minutes 20 at 4*C. The supernatants were removed from the wells, and each cell pellet was resuspended using 150 p1 of 2xYTAK per well. The plate was incubated at 30*C overnight for phagemid expression. Human Ang-2 protein was coated onto the 96 well Maxisorp plate (NUNC) at 1 pg/ml in 1xPBS at 4*C overnight. As a control, 2% BSA (Sigma) 25 was coated onto a separate Maxisorp plate. On the following day, the overnight cell cultures were centrifuged at 2000 rpm for 10 minutes at 4*C. Ten p1 of supernatant from each well was transferred to a new 96 well plate which containing BSA/PBS solution to dilute the supernatant at 1:10. The resulting mixtures were incubated for 1 hour at room temperature with shaking to block the 30 phagemids. Meanwhile, the Ang-2 protein coated plate was blocked with 400 g1 146 - 147 of 2% BSA/PBS solution per well for 1 hour at room temperature while shaking. The BSA solution was discarded, and each well was washed three times with PBS solution. After the last washing step, 1Opl of blocked phagemid solutions was added to each well of the Ang-2 protein coated plate as well as the control plate and incubated for 1 hour at room temperature 5 with shaking. The liquid was discarded, and each well was washed three times with PBST solution. One hundred pl of the HRP-conjugated anti-M13 mAb (Amersham Pharmacia Biotech) at 15,000 dilution was added to each well of the Ang-2 protein coated and control plates, and these plates were incubated for 1 hour at room temperature with shaking. The liquid was discarded again, and each well was washed three times with PBST solution. One 10 hundred pl of LumiGLO chemiluminescent substrates (Kirkegaard & Perry Laboratories, Gaithersburg, MD) were added to the wells, and each well was read by Luminoskan Ascent DLRearly machine (Labsystems, Franklin, MA). C. Sequencing of the Phage Clones 15 PCR reaction was performed using Ipl of bacteria from each well of the master plate (section 4A) as a template. The volume of each PCR mixture was 50 pl which contains 1x PCR buffer, 300 nM of each of the two primers: 5'-GTTAGCTCACTCATTAGGCAC-3' (SEQ ID NO: 256) and 5'-GTACCGTAACACTGAGTTTCG-3', (SEQ ID NO: 257); 20 200 pM dNTP, 2 mM MgCl 2 , and 2.5 U taq DNA polymerase (Roche Molecular Biochemicals). The GeneAmp PCR System 9700 (Applied Biosystems) was used to run the following program: 94*C for 5 minutes; 40 cycles of (94'C for 45 seconds, 55*C for 45 seconds, 72'C for 90 seconds); 72'C for 10 minutes; cool to 4*C. The PCR products were purified with QIAquick 96 PCR Purification Kit (QIAGEN Inc.) according to the 25 manufacturer's directions. All purified PCR products were sequenced with primer 5' TTACACTTTATGCTTCCG-3' (SEQ ID NO: 258) using the ABI 3770 Sequencer (Perkin Elmer) according to the manufacturer's directions.

5. Sequence Ranking The peptide sequences that were translated from nucleotide sequences (from section 4C above) were correlated to ELISA data. The clones that showed 5 high OD reading in the Ang-2 coated wells and low OD reading in the BSA coated wells were considered more important. The sequences that occurred multiple times were also considered important. Twenty four peptide sequences from library 20, 26 peptide sequences from library 94, 7 peptide sequences from library 25, 18 peptide sequences from library 26, 6 peptide sequences from library 10 27, and 4 peptide sequences from library 99 were chosen for further analysis and peptibody generation. Additionally, eleven consensus sequences from libraries 20 and 94, three consensus sequences from libraries 26 and 99, and two from library 25 were deduced and used to generate peptibodies. The peptibodies in Table 7 were evaluated using the Neutralization ELISA protocol described in Example 10 15 herein. The results are shown in Table 7. Table 7 Con4 Derived Affinity-Matured hAng-2:Tie2 Peptibody Sequence (Seq Id No:) Pbs

IC

50 (nM) M-Fc-GGGGGAQ PIRQEECDWDPWTCEHMWEV-LE Con4-44 (C) 0.09 (SEQ ID NO: 259) M-Fc-GGGGGAQ TNIQEECEWDPWTCDHMPGK-LE Con4-40 (C) 0.10 (SEQ ID NO: 260) M-Fc-GGGGGAQ WYEQDACEWDPWTCEHMAEV-LE Con4-4 (C) 0.12 (SEQ ID NO: 261) M-Fc-GGGGGAQ NRLQEVCEWDPWTCEHMENV-LE Con4-31 (C) 0.16 (SEQ ID NO: 262) M-Fc-GGGGGAQ AATQEECEWDPWTCEHMPRS-LE Con4-C5 (C) 0.16 (SEQ ID NO: 263) M-Fc-GGGGGAQ Con4-42 (C) 0.17 LRHQEGCEWDPWTCEHMFDW-LE .148 (SEQ ID NO: 264) M-Fc-GGGGGAQ VPRQKDCEWDPWTCEBMYVG-LE Con4-35 (C) 0.18 (SEQ ID NO: 265) M-Fc-GGGGGAQ SISHIEECEWDPWTCEBMQVG-LE Con4-43 (C) 0.18 (SEQ ID NO: 266) M-Fc-GGGGGAQ WAAQEECEWDPWTCEIMGRM-LE Con4-49 (C) 0.19 (SEQ ID NO: 267) M-Fc-GGGGGAQ TWPQDKCEWDPWTCEHMGST-LE Con4-27 (C) 0.22 (SEQ ID NO: 268) M-Fc-GGGGGAQ GHSQEECGWDPWTCEBMGTS-LE Con4-48 (C) 0.26 (SEQ ID NO: 269) M-Fc-GGGGGAQ QHWQEECEWDPWTCDHMPSK-LE Con4-46 (C) 0.26 (SEQ ID NO: 270) M-Fc-GGGGGAQ NVRQEKCEWDPWTCEHMPVR-LE Con4-41 (C) 0.26 (SEQ ID NO: 271) M-Fc-GGGGGAQ KSGQVECNWDPWTCEHjMPRN-LE Con4-36 (C) 0.28 (SEQ ID NO: 272) M-Fc-GGGGGAQ VKTQEHCDWDPWTCEHMREW-LE Con4-34 (C) 0.28 (SEQ ID NO: 273) M-Fc-GGGGGAQ AWGQEGCDWDPWTCEHMLPM-LE Con4-28 (C) 0.30 (SEQ ID NO: 274) M-Fc-GGGGGAQ PVNQEDCEWDPWTCEHMPPM-LE Con4-39 (C) 030 (SEQ ID NO: 275) M-Fc-GGGGGAQ RAPQEDCEWDPWTCAHMDIK-LE Con4-25 (C) 0.31 (SEQ ID NO: 276) 149 M-Fc-GGGGGAQ HGQNMECEWDPWTCEHMFRY-LE Con4-50 (C) 0.38 (SEQ ID NO: 277) M-Fc-GGGGGAQ PRLQEECVWDPWTCEHMPLR-LE Con4-38 (C) 0.40 (SEQ ID NO: 278) M-Fc-GGGGGAQ RTTQEKCEWDPWTCEHMESQ-LE Con4-29 (C) 0.41 (SEQ ID NO: 279) M-Fc-GGGGGAQ QTSQEDCVWDPWTCDHMVSS-LE Con4-47 (C) 0.44 (SEQ ID NO: 280) M-Fc-GGGGGAQ QVIGRPCEWDPWTCEHLEGL-LE Con4-20 (C) 0.48 (SEQ ID NO: 281) M-Fc-GGGGGAQ WAQQEECAWDPWTCDHMVGL-LE Con4-45 (C) 0.48 (SEQ ID NO: 282) M-Fc-GGGGGAQ LPGQEDCEWDPWTCEHMVRS-LE Con4-37 (C) 0.49 (SEQ ID NO: 283) M-Fc-GGGGGAQ PMNQVECDWDPWTCEIMPRS-LE Con4-33 (C) 0.52 (SEQ ID NO: 284) M-Fc-GGGGGAQ FGWSHGCEWDPWTCERMGST-LE AC2-Con4 (C) 0.52 (SEQ ID NO: 285) M-Fc-GGGGGAQ KSTQDDCDWDPWTCEHMVGP-LE Con4-32 (C) 0.75 (SEQ ID NO: 286) M-Fc-GGGGGAQ GPRISTCQWDPWTCEHMDQL-LE Con4-17 (C) 0.96 (SEQ ID NO: 287) M-Fc-GGGGGAQ STIGDMCEWDPWTCAHMQVD-LE Con4-8 (C) 1.20 (SEQ ID NO: 288) M-Fc-GGGGGAQ VLGGQGCEWDPWTCRLLQGW-LE AC4-Con4 (C) 1.54 (SEQ ID NO: 289) M-Fc-GGGGGAQ VLGGQGCQWDPWTCSIREDG-LE Con4-1 (C) 2.47 (SEQ ID NO: 290) M-Fc-GGGGGAQ Con4-C1 (C) 2.75 TTIGSMCEWDPWTCAHMQGG-LE .150 -_ (SEQ ID NO: 291) M-Fc-GGGGGAQ TKGKSVCQWDPWTCSHMQSG-LE Con4-21 (C) 3.21 (SEQ ID NO: 292) M-Fc-GGGGGAQ TTIGSMCQWDPWTCAHMQGG-LE Con4-C2 (C) 3.75 (SEQ ID NO: 293) M-Fc-GGGGGAQ WVNEVVCEWDPWTCNHWDTP-LE Con4-18 (C) 4.80 (SEQ ID NO: 294) M-Fc-GGGGGAQ VVQVGMCQWDPWTCKHMRLQ-LE Con4-19 (C) 5.76 (SEQ ID NO: 295) M-Fc-GGGGGAQ AVGSQTCEWDPWTCAHLVEV-LE Con4-16 (C) 6.94 (SEQ ID NO: 296) M-Fc-GGGGGAQ QGMKMFCEWDPWTCAHIVYR-LE Con4-11 (C) 9.70 (SEQ lID NO: 297) M-Fc-GGGGGAQ TTIGSMCQWDPWTCEHMQGG-LE Con4-C4 (C) 9.80 (SEQ ID NO: 298)

M-FC-GGGGGAQ

TSQRVGCEWDPWTCQHLTYT-LE Con4-23 (C) 9.88 (SEQ ID NO: 299) M-Fc-GGGGGAQ QWSWPPCEWDPWTCQTVWPS-LE Con4-15 (C) 15.00 (SEQ ID NO: 300) M-Fc-GGGGGAQ GTSPSFCQWDPWTCSHMVQG-LE Con4-9 (C) 20.11 (SEQ ID NO: 301) M-Fc-GGGGGAQ TQGLHQCEWDPWTCKVLWpS-LE Con4-10 (C) 86.61 (SEQ.ID NO: 302) M-Fc-GGGGGAQ VWRSQVCQWDPWTCNLGGDW-LE Con4-22 (C) 150.00 (SEQ ID NO: 03) M-Fc-GGGGGAQ DKILEECQWDPWTCQFFYGA-LE Con4-3 (C) 281.50 . (SEQ ID NO: 304) 151 M-Fc-GGGGGAQ ATFARQCQWDPWTCALGGWLE Con4-5 (C) No Inhibition (SE ID NO: 305) M-FC-GGGGGAQ. GPAQEECEWDPWTCEPLPLM-LE Con4-30 (C) No Inhibition (SEQ DD NO: 306) M-Fc-GGGjGGAQ RPEDMCSQWDPWTWPJQGYC-LE Con4-26 (C) No Inhibition (SEQ ID NO: 307) M-FC-GGGGGAQ. Con4-7 (C) No Inhibition (SEQ ID NO: 308) M-Fc-GGGGGAQ TQLVSLCEWDPWTCAJLDGW-LE Con4-12 (C) No Inhibition (SEQ ID NO: 309) M-Fc-GGGGGAQ MGGAGRCEWDPWTCQLLQGW-LE Con4-13 (C) No Inhibition (SEQ ID NO: 310) M-Fc-GGGGGAQ RLPNCQWDPWTCSLPG-LE Con4-14 (C) No Inhibition (SEQ ID NO: 311) M-Fc-GGGGGAQ FGWSHGCEWDPWTCDLQGW-LE Con4-2 (No Inhibition NO: 312) M-Fc-GGGGGAQ WPQTEGCQWDPWTCRLLDGW-LE Con-6 (C) No Inhibition (SEQ ID NO: 313) M-Fc-GGGGGAQ MGGAGCEWDPWTCLLGW-LE Con424 () NoInhiitio (SEQ I D NO: 310) M-Fc-GGGGGAQ MFPNEQDPWTCSLPEA-LE AC5-Con4 (C) No Inhibition (SEQ ID NO: 317) M-Fc-GGGGGAQ FGWSHCEWDPWTCRLLQGW-LE ACon4 (C) No Inhibition _(SEQ ID NO: 312) M-Fc-GGGGGAQ M -Fc -G G G Q ATQDKCEWDPWTCRLLQGW-LE AC5-Con4() o nhibtion(SEQ'P ID NO: 315) C)(SEQI; O:36 152 Li Derived hAng-2:Tie2 Affinity-Matured ICso (nM) Peptibody Sequence (Seq Id No:) Pbs

MGAQ

TNFMPMDDLEQRLYEQFILQQG

LEGGGGG-Fc L1-7 (N) 0.03 (SEQ ID NO: 318)

MGAQ

TNYKPLDELDATLYEHWILQHS LEGGGGG-Fc AC6-L1 (N) 0.03 (SEQ ID NO: 319)

MGAQ

QKYQPLDELDKTLYDQFMLQQG LEGGGGG-Fc LI-15 (N) 0.04 (SEQ ID NO: 320) MGAQiLNFTPLDELEQTLYEQWTLQQS LEGGGGG-Fc L1-2 (N) 0.04 (SEQ ID NO: 321)

MGAQ

QKFQPLDELEQTLYEQFMLQQA LEGGGGG-Fc L1-10 (N) 0.05 (SEQ ID NO: 322).

MGAQ

QEYEPLDELDETLYNQWMFHQR LEGGGGG-Fc L1-13 (N) 0.05 (SEQ ID NO: 323) MGAQ-VKYKPLDELDEILYEQQTFQER LEGGGGG-Fc Li-5 (N) 0.05 (SEQ ID NO: 324)

MGAQ

TKFQPLDELDQTLYEQWTLQQR LEGGGGG-Fc L1-C2 (N) 0.05 (SEQ ID NO: 325)

MGAQ

TNFQPLDELDQTLYEQWTLQQR LEGGGGG-Fc L1-C3 (N) 0.06 (SEQ ID NO: 326)

MGAQ

QNFKPMDELEDTLYKQFLFQHS LEGGGGG-Fc LI-11 (N) 0.07 (SEQ ID NO: 327) 153

MGAQ

VKYKPLDELDEWLYHQFTLHHQ LEGGGGG-Fc L1-17 (N) 0.08 (SEQ ID NO: 328)

MGAQ

YKFTPLDDLEQTLYEQWTLQHV LEGGGGG-Fc L1-12 (N) 0.08 (SEQ ID NO: 329) MGAQ-QNYKPLDELDATLYEHFIFHYT LEGGGGG-Fc Li-1 (N) 0.08 *(SEQ ID NO: 330)

MGAQ

'VKFKPLDALEQTLYEHWMFQQA LEGGGGG-Fc L1-4 (N) 0.08 (SEQ ID NO: 331)

MGAQ

EDYMPLDALDAQLYEQFILLHG LEGGGGG-Fc LI-20 (N) 0.09 (SEQ ID NO: 332)

MGAQ

YKFNPMDELEQTLYEEFLFQHA LEGGGGG-Fc LI-22 (N) 0.09 (SEQ ID NO: 333)

MGAQ

SNFMPLDELEQTLYEQFMLQHQ LEGGGGG-Fc L1-14 (N) 0.11 (SEQ ID NO: 334)

MGAQ

QKFQPLDELEETLYKQWTLQQR LEGGGGG-Fc L1-16 (N) 0.11 (SEQ ID NO: 335) MGAQ-QKFMPLDELDEILYEQFMFQQS LEGGGGG-Fc LI-18 (N) 0.16 (SEQ ID NO: 336)

MGAQ

TKFNPLDELEQTLYEQWTLQHQ LEGGGGG-Fc LI-3 (N) 0.16 (SEQ ID NO: 337)

MGAQ

HTFQPIDELEETLYYQWLYDQL LEGGGGG-Fc L1-21 (N) .0.17 (SEQ ID NO: 338) 154

MGAQ

QKFKPLDELEQTLYEQWTLQQR LEGGGGG-Fc LI-Cl (N) 0.56 (SEQ ID NO: 339)

MGAQ

QTFQPLDDLEEYLYEQWIRRYH LEGGGGG-Fc L-19 (N) 1.26 (SEQ ID NO: 340)

MGAQ

SKFKPLDELEQTLYEQWTLQHA LEGGGGG-Fc' L1-9 (N) 1.62 (SEQ ID NO: 341). Con1 Derived Affinity-Matured hAng-2:Tie2 Peptibody Sequence (Seq Id No:) Pbs

IC

5 o (nM) 3o14().0 (SQ.68O 33

M-FC-GGGGGAQ

rn SGQDLRPCEDMYGCGTNLWYGLE Con1-6 (C) 8.60 (SE ID NO: 34) M-Fc-GGGGGAQ APGQNPDGMGWPYQWILi Con1-3 (C) 16.42 (SEQ ID NO: 345) M-Fc-GGGGGAQ GQDLRPCEEGCGTNJLACL-LE Con12 (C) No60 bo(SEQ ID NO: 346) M-Fc-GGGGGAQ AGGMRPYDGMGWPYDQVQ-LE. (SEQ ID NO: 343) M-Fc-GGGGGAQ- QDRPCEMF[GCGTWYGC-LE Con 1-2 (C) No Inhibition (SEQ ID NO: 347) M-Fc-GGGGGAQ-C~ AGQRMPYDGGPTQRI-LE M-Fc-GGGGGAQ- bUEEF CYQ LEG Parent: Con1 (C) 26.00 (SEQ ID NO: 348) 155 12-9 Derived Affinity-Matured hAng-2:Tie2 Peptibody Sequence (Seq Id No:) .--. Pbs _ICso (nM) M-IFc-GGGGGAQ LQEWCEGVEDPFYFGCEKQR-LE 12-9-3 (C) 0.81 (SEQ ID NO: 349) M-Fc-GGGGGAQ MIDYCEGMDDPFTFGCDKQM-LE 12-9-7 (C) 0.93 (SEQ ID NO: 350) M-FC-GGGOGAQ. HQEYCEG4EIJPFTFGCEYQGJJE 12-9-6 (C) 0.95 (SEQ ID NO: 351)

M-FC,-GGGGGAQ

LQDYCEGVEDPFTJFGCENQR-LE 12-9-C2 (C) 1.41 (SEQ ED NO: 352) M-FCZ-GGGGAQ. LLDYCEGVQDPFT1FGCENTT-LE 12-9-5 (C) 1.56 (SEQ ED NO: 353) M-Fc-GGGGGAQ GFEYCDGMEDPFIFGCDKQT-LE 12-9-1 (C) 1.84 (SEQ ED NO: 354) M-Fc-GGGGGAQ AQDYCEGEDP~rFGCEMQK-LE 12-9-4 (C) 2.05 (SEQ ID NO: 355) 12-9-Cl C 2.68(S IDN:36 M-Fc-GGGGGAQ LQYCEGVEDPFI'FGCEKQR-LE 12-9-C2 (C) 2.68 (SEQ ID NO: 356) M-Fc-GGGGGAQ

M-FC-GGGGGAQ

HQECEMEDFTGCEQLE

FDYCEGVEDPFTFGCEN

Parent: 12-9 (C) 15.00 (SEQ ID NO: 358) Example 9 5 Six samples of anti-Ang,2 peptibodies were tested for their binding activity to huAng2 (R&D Systems, BNO121O3A) on BIAcore. Protein G was immobilized to a CM5 chip according to the standard amine-coupling protocol (BIAcore Inc.), and the peptibodies were then injected over a protein G surface for 10 capturing (RL -100 Ru). To test binding between hAng2 and the captured 156 peptibody, 0.3 nM to 40 nM of huAng2 was injected over the captured peptibody surfaces, and binding sensorgrams were analyzed using BIAevaluation 3.0 (BIAcore Inc.). Table 8 summarizes the results of this experiment. 5 Table 8 Peptibody Lot # KID (M) ka (i/Ms) kd (1/s) Con4-44 (C) 011702 2.1E-10 2.9E+05 5.9E-05 L1-7..(N) 022102 2.4E-10 3.7E+05 8.7E-05 L1-10 (N) 021302 7.7E-10 1.5E+05 1.1E-04 L1-21 (N) 021802 2.4E-10 5.6E+05 1.4E-04 Con4 (C) 33456-77 3.8E-10 5.3E+05 2.0E-04 2xCon4 (C) 1K 092501 3.4E-10 4.8E+05 1.6E-04 10 Example 10 Neutralization ELISA The human, murine, cyno, and rat Ang-2 and human and murine Ang-1 conditioned media were diluted in DMEM/50pg/ml BSA as follows: hAng-2 1:64 dilution; mAng-2 - 1:64 dilution; rat Ang-2 - undiluted; cyno Ang-2 - 1:32 15 dilution; hAng-I - 1:4 dilution; and inAng-1 - 1:4 dilution. The extent to which each of these conditioned media was diluted was determined by their ability to bind InM hTie2-Fc (provided as a Tie-2-Fc molecule where the Tie-2 portion contains only the soluble extracellular portion of the molecule; R&D Systems, catalog number 313-TI) at 50% of maximally 20 achievable binding (i.e., plateau). Microtiter plates were coated with 100ptl of the diluted conditioned media. For Ang-2 neutralization ELISAs, candidate anti-Ang 2 peptibodies were titrated from 62.5nM to 0.015pM in 4-fold dilutions in a solution of PBS containing about 1% BSA and about 1nM Tie-2 (provided as a Tie-2-Fc molecule where the Tie-2 portion contains only the soluble extracellular 25 portion of the molecule; R&D Systems, catalog number 313-TI). For Ang-1 neutralization ELISAs, candidate anti-Ang-2 peptibodies were titrated from 10OOnM to 0.2pM in 4-fold dilutions in a solution of PBS containing about 1% 157 1BSA and about lnM Tie-2 (provided as a Tie-2-Fc molecule where the Tie-2 portion contains only the soluble extracellular portion of the molecule; R&D Systems, catalog number 313-TI). After about 100 microliters of the peptibody/Tie-2 solution was added to 5 each well, the plates were incubated overnight at room temperature, and then washed five times in PBS containing about 0.1 percent Tween-20. After washing, about 100 microliters per well of anti-Tie-2 antibody (Pharmingen Inc., catalog #557039) was added to a final concentration of about 1 microgram per ml, and the plates were incubated about 1 hour at room temperature. Next, about 100 10 microliters per well of goat anti-mouse-IgG-HRP (Pierce Chemical Co., catalog #31432) was added at a dilution of 1:10,000 in PBS containing about 1% BSA. Plates were incubated at room temperature for about 1 hour, after which they were washed five times with PBS containing about 0.1 percent Tween-20. About 100 microliters per well of TMB substrate (SIGMA, catalog # T8665) was 15 then added and blue color was allowed to develop. Absorbance was then read in a spectrophotomer at 370 nm. The results are set forth in Table 9 below. Table 9 20 Peptibody-Mediated Neutralization of Angiopoietin:Tie2 Interactions hAng-2_ mAng-2 rAn -2 cAng-2 hAng-1 mAng-1 Pep tibody

IC

5 o (nM) IC 5 o (nM) ICso (nM) ICSO (nM) IC 50 (nM) ICso (nM) 2xCon4 (C) 0.026 0.035 0.024 0.047 3.0 3.2 Con4 (C) 0.197 0.289 0.236 0.540 200 300 Con4-44 (C) 0.08 0.16 0.22 ---- 43

-

Con4-40 (C) 0.20 0.27 0.35 ---- > 1000

-

L1-7 (N) 0.046 0.063 0.035 0.108 > 1000 > 1000 L1-21 (N) 0.179 0.249 0.204 0.608 >1000 >1000 L1-1 (N) 0.06 0.06 0.06 ---- > 1000 25 Example 11 PK Study 158 Study Design Male CD-1 mice, weighing 2 0-30g, were randomly divided into each peptibody treatment group (2xCon4-C, L1-7-N, and L1-21-N). Animals received a single 1V bolus (n=38/group) or a single SC administration of 50pg peptibody 5 (n=34/group). The injections were done via the tail vein and under the skin over the shoulders for IV and SC administrations, respectively. Blood Sampling and Analytical Methods Blood samples were collected for each anti-Ang2 peptibody concentration measurement predose, and at 1, 2, 4, 8, 16, 24, 48, 72, 96, 120, 144, 168, 216, 10 264, 312, and 336 hours after dose administration for the.SC and IV groups. Additional samples were collected at 5 and 30 minutes postdose for IV groups. Two animals were bled per time point, and animals were sacrificed after sampling. Blood (approximately 0.50 mL) was collected from a cardiac puncture into polypropylene microtairier@ serum separator tubes. Samples Were kept on 15 ice for approximately 20 minutes or until clot formation-occurred. Serum was separated from the blood samples by centrifugation for approximately 10 minutes at 2-8*C, and stored at approximately -70*C until assayed. Samples were measured using a verified time resolved fluorescence (TRF) assay with a lower limit of quantification (LLOQ) of 100 ng/mL. NUNC fluoroMaxisorp microtiter 20 plates were coated with recombinant mouse Ang-2 protein. The plates were then blocked with a protein solution to reduce nonspecific binding. Standards, quality controls and unknown samples were prepared in 10% mouse serum assay buffer and pipetted into wells of microtiter plates. The peptibodies were bound specifically to the immobilized Ang-2. After washing away any unbound 25 substances (Kirkegaard & Perry Laboratories Inc.), a biotinylated goat anti Human IgG (H+L) monoclonal antibody (Jackson ImmunoResearch Laboratories Inc.) was added to the wells. Following a wash step to remove-any unbound biotinylated monoclonal antibody, europium labelled streptavidin was added to the wells. After washing off the unbound streptavidin europium, the bound 30 europium was released from the streptavidin with an acidic solution pipetted into each well. Fluorescent signal was generated and read in the Wallac's fluorometric 159 reader. The assay range for the analysis of anti-Ang-2 peptibody in mouse serum is 0.078-5 jtg/mL. Pharmacokinetic Analysis The composite mean concentration-time data for each group were 5 subjected to noncompartmental analysis using WinNonlin Professional (Version 3.3, Pharsight Corp., Mountain View, CA). The nominal sampling times were used for PK analysis, as samples were collected within 10% of the nominal time. All concentration values less than the LLOQ were set to zero before PK analysis. The following PK parameters were estimated: 10 e Terminal half-life (t,). was calculated as tA -- In(2), where ki was the first ke order terminal rate constant estimated via linear regression of the terminal log-linear decay phase. - The area under the serum concentration-time curve (AUC(O.Iast)) was estimated using the linear/log trapezoidal method from time 0 to last, the 15 time of the last quantifiable concentration (Clast). * The area under the curve from time 0 to infinity (AUC(o-)) was estimated as the sum of the corresponding AUC(-last) and the predicted Ciast/kei values: AUC(O->= AUC(o-asty + Pr edicted Clast kel 20 0 Absolute bioavailability (F) after SC administration was calculated as: F = AUC(o - -)sc x1 00 AUC(o -..)iv The results are set forth in Figure 2. 160 Example 12 Female nude mice were injected subcutaneously with 1x 107 A431 cells on study day 0. At day 3, the Ang-2 peptibody 2xCon4-C was administered subcutaneously at a dose of 200 sLg/mouse/day. Tumor volumes and body 5 weights were recorded at regular intervals, as shown in the figure. Significant differences in tumor growth were observed between the Ang-2 peptibody-treated group versus vehicle control and control peptibody (p <0.0001 vs. each control using.iepeated measure ANOVA, with Scheffe's post hoc test). Treatment with this peptibody had no significant effect on body weights. the results are set forth 10 in Figure 3. Example 13' A431 In Vitro Growth Curve A431 cells were seeded in 96-well tissue culture plates at 2000 cells per 15 well, in 200ptl of DMEM supplemented with 10% fetal bovine serum (FBS). The medium was then aspirated 16 hours post seeding. The following were then added back into the wells and set up in triplicate: 10011 per well of DMEM, 10% FBS, 1mg/mI negative control peptibody 4883 or peptibody TN8-Con4. The same set-ups were repeated on 5 plates. Medium from one plate was aspirated at 24, 20 48, 72, 96, and 120 hours post treatment. One hundred pl of 10% trichloroacetic acid (TCA) per well were then added, and the plates were then stored at 4"C. All of the plates were collected when the last plate had been in 10% TCA for a minimum of 4 hours. The 10% TCA was shaken out, and the wells were rinsed 5 times with tap water. The cells were then stained with 1001 0.4% 25 sulforhodamine B (Sigma S-9012) in 1% acetic acid (Sigma A-6283) for 10 minutes at room temperature, and then washed 5 times with 1% acetic acid. The plates were then air dried. The dye was solubilized with 300g1 20mM unbuffered Tris (pH>10) for 2 hours on a rotary shaker. Optical density (OD) was then read at 540nm on a microtiter plate reader. The results are set forth in Figure 4. 30 161 Example 14 Female nude mice were injected subcutaneously with 2x10 6 Colo-205 cells plus Matrigel (2:1) on study day 0. At day 3, the Ang-2 peptibodies L1-7-N, L1-21-N, Con4-C, and 2xCon4-C were administered subcutaneously at the dose 5 of 14 jg/mouse, twice a week. Anti-Ang-2 antibody Ab536, 47 jig/mouse, three times a week, was administered as a positive control. Tumor volumes and body weights were recorded at regular intervals. . Significant differences in tumor growth were observed between each one of the Ang-2 peptibody treated group versus vehicle control and control peptibody 10 (p <0.0001 vs. each control using repeated measure ANOVA, with Scheffe's post hoc test). Treatment with these peptibodies had no significant effect on body weights (results not shown). The results are set forth in Figure 5. Example 15 15 Female nude mice were injected subcutaneously with 2x10 6 Colo-205 cells plus Matrigel (2:1) on study day 0. At day 3, the Ang-2 peptibody 2xCon4 C was administered subcutaneously at the doses of 14, 2.8, and 0.56 jg/mouse, twice a week. Tumor volumes and body weights were recorded at regular intervals, as shown. Significant differences in tumor growth were observed 20 between the two higher doses of the Ang-2 peptibody treated group versus vehicle control and control peptibody (p =0.003 for the intermediate dose and p<0.0001 for the high dose, using repeated measure ANOVA, with Scheffe's post hoc test). Treatment with these peptibodies had no significant effect on body weights. The dashed line represent a reduction of the total n of the group, from 10 to 9 mice, 25 due to the death of one mouse for unknown reasons. The results are set forth in * Figure 6. 162 - Example 16 Anti-Ang-2 peptibodies vs. Colo-205 Xenograft Tumors Female nude mice were injected subcutaneously with 2x10 6 Colo-205 .5 cells plus Matrigel (2:1) on study day 0. At day 3, Ang-2 peptibody 2xCon4-C or control peptibody were administered subcutaneously at the dose of 350 pg/day. Tumors from groups treated with control peptibody (as described in Table 5) were ; harvested either at Day 14 (size-matched control) or Day 18 (time-matched control). Tumors from 2xCon4(C) treated group were then harvested at Day 18. 10 Tumor volumes were recorded at regular intervals, as shown. Significant differences in tumor growth were observed between the time-matched control group and the 2xCon4-C treated group (p=0.0154 by repeated measure ANOVA, with Scheffe's post hoc test). Treatment with these peptibodies had no significant effect on body weight. 15 Tumors prepared for image analysis were bisected coronally and one-half snap frozen in OCT (Sakura Finetek USA Inc., Torrance, CA). Cryo-sections were immunohistochemically stained using anti-mouse CD31 (catalogue #553370, BD PharMingen, San Diego, CA) at 2 Rg/ml, with DAB as the chromogen. The tumor sections were digitally photographed at 20X objective 20 magnification. Four "compass-point" fields per tumor were captured, with ten tumors per treatment group. A MetaMorph (Universal Imaging Corporation, Downington, PA) image analysis system was used to threshold for the CD31 stained blood vessels within the images. The areas of CD31 positive staining were expressed as a ratio of the total tumor tissue within each field. The results 25 are set forth in Figure 7. Example 17 Female nude mice were injected subcutaneously with 2x10 6 Colo-205 cells plus Matrigel (2:1) on study day 0. Treatment with 350 Rg/mouse, s.c. twice 30 a week, of the Ang-2 peptibody 2xCon4-C, or equivalent control peptibody started either at study day 3, 10 or 15. Tumor volumes and body weights were recorded at regular intervals. Significant differences in tumor growth were observed between all Ang-2 peptibody treated group versus vehicle control 163 (P=0.089 for day 15 group and p<0.0001 for day 3 and 10 groups, using repeated Measure ANOVA, with Scheffe's post hoc test). Treatment with these peptibodies had no significant effect on body weights. The results are set forth in Figure 8 (body weights not shown). 5 Example 18 A summary of complete response (CR) rates was obtained using antibody Ab536 at 47 Rg/female nude mouse, administered intraperitoneally three times a week, or with peptibody 2xCon4(C), given subcutaneously at multiple dosing 10 schedules in different long term studies (> 10 weeks of dosing) in both the A431 and Colo-205 xenograft models. CR as used herein refers to an outcome in which no measurable tumor remained following treatment. The results are set forth in Figure 9. 15 Example 19 a) Combination of Pb with Taxotere in the Colo-205 Tumor Model Female nude mice were injected subcutaneously with 2x10 6 Colo-205 cells plus Matrigel (2:1) on study day 0. At study day 14, treatments were started with a) 350 [tg/mouse, s.c. twice a week, of the Ang-2 peptibody 2xCon4-C, b) 20 20 mg/kg qwx3 i.p. of taxotere, or c) a combination of both. Tumor volumes and body weights were recorded at regular intervals. Significant differences in tumor growth were observed between all treatment groups versus vehicle control (p<0.0001 using repeated measure ANOVA, with Scheffe's post hoc test). In addition, the combination therapy group was significantly different than either one 25 of the monotherapy agents (p<0.0001 vs. 2xCon-4-C and p=0.0122 vs taxotere). The dashed line represents a reduction of the total n of the group, from 10 to 9 mice, due to the death of one mouse for unknown reasons. Treatment with these peptibodies had no significant effect on body weights. The results are set forth in Figure 10a. 30 b) Combination of Pb with 5-FU in the Colo-205 Tumor Model 164 Female nude mice were injected subcutaneously with 2x10 6 Colo-205 cells plus Matrigel (2:1) on study day 0. At study day 14 started treatments with a) 350 ptg/mouse, s.c. twice a week, of.the Ang-2 peptibody 2xCon4-C, b) 50 mg/kg qdx5 i.p. of 5-FU, or c) a combination of both. Tumor volumes and body 5 weights were recorded at regular intervals, as shown. Significant differences in tumor growth were observed between all treatment groups versus vehicle control (p<0.0001 using repeated measure I ANOVA, with Scheffe's post hoc test). In addition, the combination therapy group was significantly different than either one of the monotherapy agents 10 (p=0.0375 vs. 2xCon-4-C and p=0.0453 vs. 5-FU). A transient reduction in body weight was observed in the 5-FU treated group (18% at study day 20) as well as with the combination therapy group (16% at study day 20), followed by a complete recovery of the body weights. The results are set forth in Figure 10b. 15 Example 20 Adiuvant Arthritis Model Male Lewis rats ( 1 2 0-130g, Charles River, Wilmington MA) were housed two per filter-capped cage.in an environmentally controlled room (temperature 23 2*C, relative humidity 50 ± 20%) on a 1 2-hourlight/dark cycle. Animals were 20 fed a commercial rodent chow (Formulation 8640; Tek Lab, Madison, WI) and received filter-purified tap water ad libitum. Dietary calcium and phosphorus contents were 1.2% and 1.0%, respectively. Adjuvant arthritis was induced by a single 0.5mg injection of heat-killed Mycobacterium tuberculosis H37Ra (Difco Laboratories, Detroit, MI) suspended 25. in 0.05 mL paraffin oil (Crescent Chemical Co., Hauppauge, NY) intradermally at the base of the tail. The clinical onset of arthritis was at day 9 as indicated by hind paw swelling and ambulatory difficulties. Except in the 2xCon4(c) treated group (which was treated from Day 1 after immunization), treatments were given as daily subcutaneous injections beginning at day 9 after immunization (prior to 30 onset of arthritis) and continuing through day 18. Clinical Monitorin of Adjnvant Arthritis. 165 The progression of inflammation was assessed clinically by the intermittent measurement of hind paw volume using water plethysmography according to the methods described by Feige et al., Cellular Molec. Life Sci., 57:1457-1470 (2000). Inhibition of paw inflammation was calculated based on 5 the area under the curve (AUC) using the trapezoidal rule according to the formula: [1 - {(Treated AdA) - normal) / (Untreated AdA - normal)}] x 100 In addition, total body weight was determined daily during the 9-day treatment regimen as a supplemental endpoint because body weight loss has been 10 shown to parallel the progression of joint inflammation in this arthritis model. Animals were sacrificed under CO 2 on day 18. Loss of bone mineral density (BMD) was examined at necropsy (day 18 post immunization). Hind paws were removed at the fur line (just proximal to the ankle (hock)), immersed in 70% ethanol, and then scanned in horizontal 15 orientation using a fan beam X-ray densitometer (Model QDR-4500A; Hologic, Waltham, MA). See Feige et al., supra. After the scan, a rectangular box (29x25 mm) centered at the calcaneus was positioned to delineate the site to be analyzed, and proprietary algorithms (Hologic software) calculated bone area, bone mineral content, and bone mineral density. 20 All results were expressed as the mean ± standard error. A p value of 0.05 was used to delineate significant differences between groups. A Kruskal-Wallis ANOVA and a Mann-Whitney U. test using commercial statistical software (Statsoft v3.0; Statsoft, Tulsa, OK) were performed on the clinical data (continuous variables). 25 The results are set forth in Figure I la, 11b, and 1 1c, respectively. 166 Example 21 Comeal Angiogenesis Model*. Effect of CON4(C) on VEGF-Induced Angiogenesis in Rats 5 Ang-2 peptibody CON4(C) was evaluated in the corneal model of angiogenesis in rats. Angiogenesis was induced by implanting a VEGF- (or BSA control) soaked nylon disc into the corneal stroma (n=8/group). Peptibody TN8CON4-C was administered by sub-cutaneous injection at 1.0 or 0.1mg/rat/day for seven days. Two other groups of animals were treated with the same dose of 10 negative control peptibody 4883. All groups were pre-treated with a single loading dose of either 3.0 or 0.3mg that was three times the maintenance dose of 1.0 or 0.1mg (see figure). After seven days of treatment, two vascular endpoints were determined from each digital image of the rat cornea: the number of vessels intersecting the mid-point between the disc and the limbus, and the blood vessel 15 area. Treatment with TN8CON4-C significantly inhibited VEGF-induced angiogenesis in a dose-dependent manner (p<0.04), whereas treatment with the control peptibody had no significant effect on either end-point. There was no evidence of overt toxicity based on body weights of the treated animals. The results are set forth in Figure 12. 20 Example 22 Epitope Mapping Full-length (amino acids 1-495), N-terminal (amino acids 1-254) and C terminal (amino acids 255-495) human Ang-2 (hAng-2) proteins were cloned into 25 a CMV-driven mammalian expression vector with C-terminal 6xHis tags. The three resultant constructs plus a vector control were transiently expressed into 293T cells. Conditioned media were then collected from the transfected cells, and the expression level of Ang-2 in the media was estimated by anti-6xhis ELISA and Western blotting. 30 The binding epitope of anti-Ang-2 antibodies and peptibodies was determined by their ability to bind the three versions of human hAng-2 by ELISA 167 according to the following protocol: a high-binding 96-well assay plate was coated with 100 p of conditioned media per well, and incubated at 37"C for 1 hour. Conditioned media was aspirated, and the plate was blocked with 200 pl per well of 5% BSA in PBS at room temperature for 1 hour. The blocking 5 solution was then aspirated. 100 p.1 per well of antibody, peptibody, or Tie2-Fc was added at lpg/ml in 1% BSA in PBS, and incubated at room temperature for I hour. The wells were washed 4 times with 200 [L1 of 0.1% Tween in PBS. 100 p.1 per well of BRP-conjugated goat anti-human IgG or goat anti-mouse IgG were added, and incubated at room temperature for 45 minutes. The wells were then 10 washed with 200 pl of 0.1% Tween in PBS 4 times. 100 [1 per well of TMB substrate was then added. O.D. was read at 3 70nm. The results are set forth in Figure 13a, Figure 13b, and Figure 13c. Example 23 15 Due to certain sensitivity limitations inherent in the BiaCore assay, binding affinity was also evaluated using a Sepidyne KinExA assay. Binding of 2xCON4-C (Pb5714) to huAng-2 was tested on KinExA (Sapidyne, Boise, ID). Reacti-Gel 6x beads (Pierce, Rockford, IL) were pre coated with huAng-2 and blocked with BSA. 10 pM and 30 pM of 2xCON4-C 20 samples were incubated with various concentrations (0.3 pM - 3 nM) of huAng-2 at room temperature for 8 hours before run through the huAng-2-coated beads. The amount of the bead-bound peptibody was quantified by fluorescent (Cy5) labeled goat anti-human-Fc antibody (Jackson Immuno Research, West Grove, PA). The binding signal is proportional to the concentration of free peptibody at 25 equilibrium. The dissociation equilibrium constant (KD) was obtained from nonlinear regression of the competition curves using a dual-curve one-site homogeneous binding model (KinExTM software). KD was then determined to be approximately 2 pM for 2xCON4-C binding with huAng-2. 30 As is shown in Figure 14, using the KinExA assay peptibody 2xCon4 was shown to have -2 pM affinity for hAng-2. 168 Example 24 Pegylated Peptides LI-7 peptide was synthesized with a 431 ABI synthesizer using a standard 5 coupling protocol and double coupling from residue 14 (met) to the N-term residue I (Cys), numbering from the N-terninus to the C-terminus. Conjuiation of LI-7 Peptide with Methoxy-poly(ethylene glycol)-naleimide; MW: 5 KDa; ermed "mPEG5K-(LI-7 Peptide)" 10 A solution of 0.8 mg of L1-7 peptide in 400gL of buffer 1 (20 mM phosphate, 5 mM EDTA, pH 6.5) was treated with 13.5 mg of methoxy poly(ethylene glycol)-maleimide (MW = 5KDa; Shearwater Corp.); 0.27 ml of a 50.0 mg/mL solution in buffer 1. The reaction mixture was incubated at 4 0 C overnight, then diluted with 1.6 mL of buffer A (20 mM Tris hydrochloride, pH 15 7.2) and dialyzed in a Slide-A-Lyzer cassette (3500 MWCO, Pierce) against the same buffer. The dialyzed reaction mixture was purified by ion exchange chromatography on a 1.0 mL HiTrap Q Sepharose HP column (Amersham Biosciences Corp.). The product peak was eluted in two 1.0 mL fractions via a gradient from 100% buffer A to 100% buffer B (buffer A + 0.5 M NaCl) over 40 20 column volumes. The combined product fractions were concentrated to 250 L containing 0.23 mg protein/mL with a Microsep 1K Centrifugal Device (Pall Life Sciences). .Coniugation of LI-7 Peptide with 1,11 -bis- maleimidotetraethyleneglycol termed 25 'TEO4(L1-7 Peptide)," A solution of 1.0 mg of L1-7 peptide in 500 pL of buffer 1 (20 mM phosphate, 5 mM EDTA, pH 6.5) was treated with 0.0375 mg of 1,1 1-bis maleimidotetraethyleneglycol (Pierce) (0.375 mL of a 0.1 mg/mL solution in btaffer 1). The reaction mixture was incubated at 4"C for 3.33 hrs, then dialyzed 30 in a Slide-A-Lyzer cassette (3500 MWCO, Pierce) against buffer A (20 mM Tris hydrochloride, pH 7.2). The dialyzed reaction mixture was purified by ion exchange chromatography on a 1.0 mL HiTrap Q Sepharose HP column (Amershan Biosciences Corp.). The dimeric product peak was eluted in three 1.0 169 mL fractions via a gradient from 100% buffer A to 100% buffer B (buffer A + 0.5 M NaCl) over 40 column volumes. The combined product fractions were concentrated to 550 gL containing 0.12 mg protein/mL with a Microsep 1K Centrifugal Device (Pall Life Sciences). 5 Coniugation of L1-7 Peptide with Polyethylene glycol)-bis-maleimide: MW 3.4 KDa: termed "PEG3.4K(L1-7 Peptide) 2 " A solution of 3.0 mg of L1-7 Peptide in 1.5 m-L of buffer 1 (20 mM phosphate, 5 mM EDTA, pH 6.5) was treated with 1.125 mg of poly(ethylene 10 glycol)-bis-maleimide (MW = 3.4 KDa, Shearwater Corp.); 0.563 mL of a 2.0 mg/mL solution in buffer 1. The reaction mixture was incubated at 4*C for overnight, then dialyzed in a Slide-A-Lyzer cassette (3500 M7WCO, Pierce) against buffer A (20 mM Tris hydrochloride, pH 7.2). The dialyzed reaction mixture was purified by ion exchange chromatography on a 5.0 mL HiTrap Q 15 Sepharose HP column (Amersham Biosciences Corp.). The product peak was eluted in three 3.0 mL fractions via a gradient from 100% buffer A to 100% buffer B (buffer A + 0.5 M NaCl) over 40 column volumes. The combined product fractions were concentrated to 850 LL containing 0.24 mg protein/mL with two Microsep 1K Centrifugal Devices (Pall Life Sciences). 20 MALDI-TOF mass spectroscopy results were as follows: Sample# Identity Exp. MS Obs. MS 1 L1-7 (unPEGylated Peptide) 3,545 3,538.7 2 mPEG5K-(L-7 Peptide) 8,500 8, 851 3 PEO4(L1-7 Peptide) 2 7,443 7,446.29 4 PEG3.4K(L1-7 Peptide) 2 10,550 10,552 6,882.61 3,550.13 It will be appreciated that the subscripted "2" for the PEG3.4K(L1-7 Peptide) and PEO4(L1-7 Peptide) indicates that there are two peptides per polymer chain, one located on each end of the polymer. 25 ICso Determination 170 The IC 5 o for inhibition of hAng2:hTie2-Fc interaction for the L1-7 free and PEGylated peptides were determined by the Neutralization ELISA as described in Example 2. For the Neutralization ELISA, microtiter plates to which human Ang-2 polypeptide was bound were prepared as described in Example 2 5 for the Affinity ELISA. Candidate anti-Ang-2 L1-7 PEGylated and Free petides were titrated from 10OOnM to 0.2pM in 4-fold dilutions in a solution of PBS containing about 1% BSA and about InM Tie-2 (provided as a Tie-2-Fc molecule. where the Tie-2 portion contains only the soluble extracellular portion of the molecule; R&D Systems, catalog number 313-TI). After about 100 microliters of 10 the'antibody/Tie-2 solution was added to each well, the plates were incubated overnight at room temperature, and then washed five times in PBS containing about 0.1 percent Tween-20. After washing, about 100 microliters per well of anti-Tie-2 antibody (Pharmingen Inc., catalog # 557039) was added to a final concentration of about 1 microgram per ml, and the plates were incubated about 1 15 hour at room temperature. Next, about 100 microliters per well of goat anti mouse-IgG-HRP (Pierce Chemical CO., catalog # 31432) was added at a dilution of 1:10,000 in PBS containing about 1 percent BSA. Plates were incubated at room temperature for about I hour, after which they were washed five times with PBS containing about 0.1 percent Tween-20. About 100 microliters per well of 20 TMB substrate (described above) was then added and color was allowed to develop. Absorbance was then read in a spectrophotomer at 370 nm. L1-7 peptides (C-GGGGG-AQ-TNFMPMDLEQRLYEQFILQQG-LE) (SEQ ID NO: 359) included: an N-terminal Cysteine for coupling to PEG; and a 5Gly linker. AQ and LE flanking sequences were present both in the original 25 phage clone and in the peptibody. The hAng-2:Tie2 Inhibition

IC

5 o results were as follows: 171 Peptide ICso (nM) L1-7 Peptide 0.49 mPEG5K-(L1-7 Peptide) 11.7 PEO4(L1-7 Peptide) 2 0.064 PEG3.4K(LI-7 Peptide) 2 0.058 5 172

Claims (20)

1. A polypeptide capable of binding Ang-2 comprising an amino acid sequence of the formula: RPe'e'e'eee7G 5 (SEQ ID NO: 73) wherein e5 is a neutral polar or an acidic amino acid residue; e 7 is a neutral hydrophobic amino acid residue, wherein further 10 e3 is Y or C, e 4 is E, e6 is M, or a physiologically acceptable salt thereof.

2. A polypeptide capable of binding Ang-2 comprising an amino acid sequence of the 15 formula: ef' fRPf f ff'f 4 Gf' 4 ffi 6 f17f18f 1 9 0 (SEQ ID NO: 74) wherein, f' is a neutral hydrophobic or neutral polar amino acid residue; 20 f9 is a neutral hydrophobic or neutral polar amino acid residue; f is a neutral polar or acidic amino acid residue; f4 is a neutral hydrophobic or neutral polar amino acid residue; f 7 is a neutral polar amino acid residue; f is an acidic amino acid residue; 25 f is a neutral polar or acidic amino acid residue; fe 0 is a neutral hydrophobic amino acid residue; f" is a neutral hydrophobic amino acid residue; f is a neutral hydrophobic or neutral polar amino acid residue; 4 is a neutral hydrophobic or neutral polar amino acid residue; 30 f 5 is a neutral polar amino acid residue; f!6 is a neutral polar amino acid residue; f" is a neutral polar or acidic amino acid residue; - 174 f 8 is a neutral hydrophobic or basic amino acid residue; fe 9 is a neutral hydrophobic or neutral polar amino acid residue; and ?0 is a neutral hydrophobic or neutral polar amino acid residue, or a physiologically acceptable salt thereof. 5

3. The polypeptide according to claim 2 wherein: f' is S, A, or G; f2 is G, Q, or P; e is Q, G, or D; f 4 is L, M, or Q; 10 f'is C or Y; is E or D; f) is E, G, or D; f' is I or M; f" is F or L; 15 f1 is C or W; f" is G or P; fe T or N; f 16 is Q, Y, or K; f1 7 is N, D, or Q; 20 fe is L, V, W, or R; f 9 is A, Q, Y, or I; and e0 is L, A, G, or V, or a physiologically acceptable salt thereof.

4. A polypeptide comprising at least one amino acid sequence selected from the group 25 consisting of SEQ ID NO: 3, and SEQ ID NOs: 143, 145, 146 and 148, inclusive, wherein said polypeptide is capable of binding to Ang-2, or a physiologically acceptable salt thereof; - 175 Peptide SEQ ID NO: Sequence Conl-1 143 AGGMRPYDGMLGWPNYDVQA Conl-3 145 APGQRPYDGMLGWPTYQRIV Conl-4 146 SGQLRPCEEIFGCGTQNLAL Conl-6 148 GQDLRPCEDMFGCGTKDWYG Coni 3 KRPCEEIFGGCTYQ

5. A polypeptide capable of binding Ang-2 comprising the amino acid sequence SEQ ID NO: 144, or a physiologically acceptable salt thereof.

6. A polypeptide capable of binding Ang-2 comprising the amino acid sequence of SEQ ID NO. 147, or a physiologically acceptable salt thereof. 5

7. A fusion polypeptide comprising at least one polypeptide according to any one of claims I to 4 and a vehicle, wherein said fusion polypeptide is capable of binding to Ang-2, or a physiologically acceptable salt thereof.

8. The fusion polypeptide according to claim 7 wherein said vehicle is at least one of an Fc domain, polyethylene glycol, a lipid, a cholesterol group, a carbohydrate, and an 10 oligosaccharide.

9. A polypeptide having the formula: (X)a-F 1 -(X 2 )b and multimers thereof, wherein: F' is a vehicle; 15 X1 and X2 are each independently selected from -(L )c-PI; -(L')c-P'-(L2dp2 -(L 1 )c-P 1 -(L 2 )d_p2 (L 3 )ep 3 ; and -(LI)c-P I(L2),_p2_ 3 ) 4P4-(L)P4 20 wherein one or more of P', P 2 , p 3 , and P 4 each independently comprise a polypeptide according to any one of claims 1 to 4; L', L 2 , L', and L 4 are each independently linkers; and - 176 a, b, c, d, e, and f are each independently 0 or 1, provided that at least one of a and b is 1, or a physiologically acceptable salt thereof.

10. The polypeptide of claim 9, wherein F' is an Fc domain or fragment thereof. 5

11. The polypeptide of claim 9 wherein the Fe domain or fragment thereof comprises the amino acid sequence of SEQ ID NO: 60.

12. A polynucleotide encoding the polypeptide or fusion polypeptide according to any one of claims I to 11.

13. A host cell comprising a polynucleotide of claim 12. 10

14. A pharmaceutical composition comprising an effective amount of the polypeptide or fusion polypeptide according to any one of claims 1 to 11, in admixture with a pharmaceutically acceptable carrier thereof.

15. A method of inhibiting angiogenesis in a mammal comprising administering to the mammal an effective amount of the polypeptide or fusion polypeptide according to 15 any one of claims 1 to 11, or the pharmaceutical composition according to claim 14.

16. A method of treating a tumor or cancer in a mammal comprising administering to the mammal an effective amount of the polypeptide or fusion polypeptide according to any one of claims I to 11, or the pharmaceutical composition according to claim 14.

17. A method of treating at least one of ocular neovascular disease, hemangioblastoma, 20 hemangioma, inflammatory disease, chronic inflammatory disorder, endometriosis, neoplastic disease, bone-related disease, or psoriasis in a mammal comprising administering to the mammal an effective amount of the polypeptide or fusion polypeptide according to any one of claims 1 to 11, or the pharmaceutical composition according to claim 14. 25

18. The method according to claim 17 wherein the inflammatory disease is arthritis.

19. Use of the polypeptide or fusion polypeptide according to any one of claims 1 to 11, for the preparation of a medicament for: - 177 inhibiting angiogenesis in a mammal; treating a tumor or cancer in a mammal; or treating at least one of ocular neovascular disease, hemangioblastoma, hemangioma, inflammatory disease, chronic inflammatory disorder, 5 endometriosis, neoplastic disease, bone-related disease, chronic inflammatory disorder, or psoriasis in a mammal.

20. A polypeptide; a fusion polypeptide; a polynucleotide; a host cell; a pharmaceutical composition; a method of inhibiting angiogenesis; a method of treatment; or use of a 10 polypeptide or fusion polypeptide, substantially as herein described with reference to any one of more of the examples but excluding comparative examples.