AU2005331250A8 - Compositions and methods for mucosal vaccination - Google Patents

Description

27-11- 06 14:84 FROM-DCC SYDNEY +61292621080 T-090 P010/046 F-074 COMPOSITIONS AND METHODS FOR MUCOSAL VACCINATION Background Classical injection vaccination routes subcutaneous, intramuscular, and intravenous are primarily concerned with the induction of systemic immunity (blood serum antibodies and T cells). While this approach may be appropriate against diseases caused by infectious agents which gain systemic access to the body through punctured or damaged skin tetanus), most pathogens naturally infect hosts through mucosal routes such as, for example, oral, nasal, or urogenital mucosa.

Injectable vaccines are generally ineffective for eliciting ixnaunity at mucosal surfaces, which is typically mediated through the production and secretion of IgA and secreted gA (s-gA),which is secreed i (s w i ted into the lumen of the intestinal, respiratory, and urinary tract, often with the secretion products of various glandular tissues. In these secretions, s-IgA is able to bind to the pathogen, which allows immune cells to eliminate the pathogen before the pathogen can begin to infect cells of the host, Thus, mucosal vaccination can substantially reduce the likelihood of a pathogen infecting host cells cellular infection) and, in some cases, even prevent a pathogen from infecting host cells.

In contrast, injected vaccines often respond to antigens released as a result of host cell infection by the pathogen lysis of infected cells). Thus, one important distinction between mucosal vaccination and injected vaccination is that mucosal vaccination can stimulate a host's defenses to limit or even prevent cellular infection, whereas injected vaccination responds to a consequence of cellular infection, hopefully before the infectious disease develops.

Mucosal vaccines are likely to be more effective at preventing or limiting mucosal infections due to their ability to induce an s-lgA response. In addition, mucosal vaccines offer several other advantages over injectable vaccines. These advantages include easier administration, reduced side effects, administration, is non-invasive does not require needles), and the potential for almost unlimited frequency of boosting without the need for trained personnel. These advantages can. reduce the cost and increase the safety of vaccinations and improve compliance, issues especially important in the developing 1 27/11 '06 MON 15:07 [TX/RX NO 5735] 27-11-' 06 14:04 FHOM-DCC SYDNEY *61292621088 T-090 P011/046 F-074 world. Furthermore, improvements in the design of novel mucosal vaccination systems may allow the development of vaccines against diseases that are currently poorly controlled.

Additionally, induction of a mucosal immune response at one mucosal site may result in an immune response at a distant mucosal site. For example, nasal or oral mucosal vaccination can generate secretion of s-lgA and IgG from the vaginal mucosa, Despite the important advantages of immunizing through mucosal routes, success with mucosal immunizations has been limited due to many factors including, for example, degradation of antigens, limited adsorption and interaction with nonspecific host factors at mnucosal sites, a lack of safe and effective adjuvants, and the use of inadequate delivery systems. There is a substantial ongoing need to expand the utility and efficacy of mucosal vaccines.

Summary It has been found that certain small molecule immune response modifiers (IRMs) can be useful as components of pharmaceutical combinations suitable for mucosal delivery.

Accordingly, the present invention provides a pharmaceutical combination that includes an IRM compound formulated for mucosal administration, and an antigen fbrmulated for mucosal administration. In some embodiments, the JRM compound and the antigen may be provided in a single formulation, while in other embodiments, the IRM compound and antigen may be provided in separate formulations.

In another aspect, the present invention also provides a method of immunizing a subject. Generally, the method includes administering an antigen to a mucosal surface of the subject in an amount effective, in combination with an IRM compound, to generate an immune response against the antigen; and administering an IRM compound to a mucosal surface of [he subject in an amount effective, in combination with the antigen, to generate an immune response against the antigen.

In some embodiments, the method may frther include one or more priming doses of antigen, one or more booster doses of antigen or IRM compound, or both.

Various other features and advantages of the present invention should become readily apparent with reference to the following detailed description, examples, claims and 27/11 '06 MON 15:07 [TX/RX NO 57351 27-11-' 26 4:4 FRO-DCC SYDNEY +61292621080 T-090 P012/046 F-74 appended drawings. In several places throughout the specification, guidance is provided through lists of examples In each instance, the recited list serves only as a representative group and should not be interpreted as an exclusive list Brief Descrption of thepDrwns Figure 1 is flow cytometry data showing proliferation of antigen-specific T cells in lymphatic tissues (NALT, Fig. IA; ILN, Fig. 1B; CLN, Fig. I C; spleen Fig. ID) after vaccmation.

Figure 2 is data showing the total number of antigen-specific T cells in lymphoid tissues (Fig. 2A-2C) and showing the percentage of antigen-specific T cells in the nasal mucosa (Fig. 2D) after vaccination; Figure 3 is flow cytoinetry data demonstrating the expansion of antigen-specific CD8S" T cells (Fig. 3Ai) and CD4' T cells (Fig. 3B) after vaccination.

Figure 4 is data showing lung lavage IgA (Fig. 4A), nasal laage IgA (Fig. 4B), and serum LeG (Fig. 4C) antibody responses to immunization via various routes with a combination of IRM and antigen.

Figure 5 is data showing lung lavage IgA (Fig. 5A) and serum IgG2b (Fig. 52) antibody responses to intranasal administration of antigen alone or with various JIPM compounds.

Figure 6 is data showing the of antigen-specific T cells in the DEN (Fig. GA) and spleen (Fig. GB) after immunization with antigen and one of various IRM compounds.

Figure 7 is data showing the of antigen-specific T cells in the DEN (Fig. 7A), and NALT (Fig 7) when immunized twice five months apart through various routes with antigen and IRM compound.

Detaied Descripoton of Illustrative Emnbodiments of the Invention Inmmune response modifiers (IRMs) are compounds that can possess potent immunomodulating activity. IAs appear to act through basic immune system mechanisms known as Toll-like receptors (TEs) to selectively modulate cytokine biosynthesis. For example, certain RM compounds induce the production and secretion of certain cytokines such as, Type I interferons, TN.-a, IL-I, IL-6, T-8, F.L-IS0, IL- 3 27/11. '06 MON 15:07 [TX/RX NO 5735] 27-1f-1 06 14:04 FROM-DCC SYDNEY +61292621080 T-090 P013/046 F-874 12, MIP-1, and/or MCP-. As another example, certain IRM compounds can inhibit production and secretion of certain TF2 cytokines, such as IL-4 and IL-5. Additionally, some IRM compounds are said to suppress IL-I and TNF Patent No. 6,518,265).

Certain IRMs may be useful for treating a wide variety of diseases and conditions such as, for example, certain viral diseases human papilloma virus, hepatitis, herpes), certain neoplasias basal cell carcinoma, squamous cell carcinoma, actinic keratosis, melanoma), and certain Tu2-mediated diseases asthma, allergic rhinitis, atopic dermatitis).

The present invention relates to pharmaceutical combinations that can be effective for use as mucosal vaccines and methods that include administering such a combination to a mucosal surface. Generally, a pharmaceutical composition according to the invention includes an IRM compound and an antigen, each formulated in a manner suitable for mucosal delivery and each in an amount that, in combination with the other, can raise an immune response against the antigen. The benefits of mucosal vaccination are many; the compositions and methods of the invention may provide one or more of the following: 1) The composition may be easily administered without the need for needles; 2) Mucosal vaccination can generate both a mucosal and a systemic immune response, whereas injected vaccines generally induce only a systemic response. Because most pathogens infect a host at a mucosal surface, mucosal vaccination induces an immune response at the site of pathogen entry; and 3) Mucosal vaccination can induce an immune response at a mucosal site other than the vaccination site.

Components of such a pharmaceutical combination may be said to be delivered "in combination" with one another if the components are provided in any manner that permits the biological effect of contacting one component with cells to be sustained at least until another component is contacted with the cells. Thus, components may be delivered in combination with one another even if they are provided in separate formulations, delivered via different routes of administration, and/or administered at different times.

For example, an rRM compound and an antigen may be considered a pharmaceutical combination regardless of whether the components axe provided in a single formulation or the antigen is administered in one formulation and the IRM compound is administered in a second formulation. When administered in different formulations, the 4 27/11 '06 MON 15:07 [TX/RX NO 57351 27-11-'86 14:05 FROM-DCC SYDNEY .61292621282 T-292 P214/846 F-274 components may be administered at different times, if desired, but administered so that the immune response generated is greater than the immune response generated if either the antigen or the IRM compound is administered alone.

In some embodiments, the pharmaceutical combination may include an IRM/antigen conjugate in which at least one IRM moiety is covalently attached to an antigen. Methods of preparing such IRM/'antigen conjugates are described, fof example, in U.S. Patent Publication No. 2004/0091491.

One method of measuring an inmmune response induced by a mucosal vaccine is to measure the expansion of antigen--specific CD8' T cells in response to challenge with the antigen. This is shown in Example 1. Antigen-specific CDS8* T cells were fluorescently labeled and adoptively transferred into syngenic mice. The mice were challenged with an IRM-antigen conjugate. Four days later, lymphoid tissue from various sites (nasal associated lymphatic tissue (NALT), cervical lymph node and spleen) was removed and the expansion of antigen-specific CD8 T cells was measured. In the tissue from each site, expansion of CD8* T cells was greater as a result of intranasal immnization than as a result of intravenous immunization with the IRM-antigen conjugate, or intranasal immunization with either antigen or 1RlMv (Fig, 2A-2C). Likewise, a greater percentage of antigen-specific CD8" T cells were observed in the nasal mucosa seven days after intranasal immunization with the IRM-antigen conjugate than were observed following either intravenous immunization with the IRM-antigen conjugate, or intranasal immunization with either antigen or IRM (Fig. 2D). Similar results were found using nonconjugated IRM and antigen (Fig. 3A).

Another method of measuring an immune response induced by mucosal vaccination is to measure expansion of antigen-specific CD4t T cells in lyrphoid tissue such as, for example, nasal associated lynmphoid tissue. Activated antigen-specific CD4' T cells, in turn, stimulate B cells to produce antibodies s-IgA) directed against the antigen. In Example 2, antigen-specific T cells were adoptively transferred into host mice.

The mice were challenged with a combination of IRM compound and an inununogenic antigen peptide. Three days later, lymphoid tissue was removed from the mice and expansion of antigen-specific CDt4 T cells was analyzed. Results are shown in Figure 3B. Expansion of CD4 T cells was greater in mice immunized with 1RM and antigen than in mice immunized with antigen alone.

27/11 '06 MON 15:07 [TX/RX NO 57351 27-11-' 86 14:85 FROM-DCC SYDNEY .61292621088 T-898 P815/846 F-274 Thus, a mucosal route of vaccination intranasal) can proide a g-eater number of antigen-specific CD8' T cells and/or CD4 T cells at relevant tissue sites the nasal associated lymphoid tissue (NALT) and the nasal mucosa compared to either nonrmucosal route of delivery (intravenous), or niucosal delivery of either antigen alone or I1kMl alone. Expansion of the antigen--specific T cell population at mnucosal sites indicates activation of iunrure cells in those locations and the generation of an immnune response that can protect against infection. When both antigen-specific CD8 T cells and antigenspecific CD4' T cells are activated, both an antigen-specific cell-mediated immune response and an antigen-specific antibody immune response may be generated.

The antigen can include any material that raises a mucosal immune response.

Suitable antigenic materials include but are not limited to proteins; peptides; polypeptides; lipids; glycolipids; polysacchaxides; carbohydrates; polyomcleotides; prins; live or inactivated bacteria, viruses or fungi; and bacterial, viral, fungal, protozoal, tumor-derived, or organism-derived inununogeris, toxins or toxoids. Additionally, as used herein, an antigen may include an oligonucleotide sequence that does not necessarily raise a mucosal irunune response itself, but can be expressed in cells of the host to produce an antigenic protein, peptide, or polypeptide. Such oligonucleotides are usefui, for example, in DNA vaccines. In some embodiments, the antigen may include a combination of two or more antigeeic materaal& Conditions fox which a composition that includes an IM and an antigen, each formulated fom mucosal administration may be useful include, but are not limited to: viral diseases such as, for example, diseases resulting from infection by an adenovirus, a herpesvirus 1S V-I, HSV-II, CMV, or VZV), a poxvirus an orthopoxvirus such as variola or vaccinia, or molluscurn contagiosum), a picornavirus rhinovimus or enterovirus), an orthomyxovirus influiezavins), a paramnyxovirus parainfluenzavirus, mumps virus, measles virus, and respiratory ayncyial virus a corornavirus SARAS), a papovavinis papillomavinises, such as those that cause genital warts, common warts, or plantar warts), a hepadnavirus hepatitis B virus), a flavivirus (e hepatitis C virus or Dengue virus), or a retrovirus a lentivirus such as IVW); bacterial diseases such as, for example, diseases resulting from infection by bacteria of, for example, the genus Escherichia, Enterobacter, Salmonella, Staphylococcus, 6 27/11 '06 MON 15:07 [TX/RX NO 57351 27-11-'06 14:05 FBOl-DCC SYDNEY +61292621080 T--090 016/046 F-74 Shigella, Listeria, Aerobacter, H-elicobacter, Klebsiella, Proteus, Pseudomonas, Streptococcus, Chlamydia, Mycoplasma, Pneumococcus, Neisseria, Clostridium, Bacillus, Corynebacterium, Mycobacterium, Campylobacter, Vibrio, Serratia, Providencia, Chromobacteium, Brucella, Yersinia, Haenophilus, or Bordetella; other infectious diseases, such chlamydia, fiungal diseases including but not limited to candidiasis, aspergillosis, histoplasmosis, cryptococcal meningitis, or parasitic diseases including but not limited to malaria, pneunmocystis carii pneumonia, leishmaniasis, cryptosporidiosis, toxoplasmosis, and trypanosone infection; and TH2-rnediated, atopic diseases, such as atopic dermatitis or eczema, eosinophilia, asthma, allergy, allergic rhinitis, and Ommen's syndrome.

For example, a mucosally administered composition may be used for prophylactic or therapeutic protection against, for example, BCG, cholera, plague, typhoid, hepatitis A, hepatitis B, hepatitis C, influenza A, influenza B, parainfluenza, polio, rabies, measles, mumps, rubella, yellow fever, tetanus, diphtheria, hemophilus influenza b, tuberculosis, meningococcal and pneumococcal vaccines, adenovirus, HIV, chicken pox, cytomegalovirus, dengue, ifeline leukemia, fowl plague, HSV-1 and fISV-2, hog cholera, Japanese encephalitis, respiratory syncytial virus, rotavifrus, papilloma virus, and Alzheimer's Disease.

In some cases, mucosal vaccination may be useful for decreasing the likelihood of, or even preventing, infection across a mucosal surface. In other cases, a mucosal vaccine may be useful for stimulating a serun antibody response. In somne cases, a mucosal vaccine may provide both protection against nmucosal infection and a serum antibody response. Thus, mucosal vaccination may be useful for vaccination against pathogens that do not typically infect across a mnucosal surface.

In ome embodiments, the antigen may be administered in one or more separate "priming" doses prior to administration of the antigen-IRM combination. Priming in this way may provide an increased immune response upon administration of the antigen-WIRM combination.

In other embodiments, the antigen may be administered in one or more separate "booster" doses after administration of the antigen-IM combination. Boosting in this way may reinvigorate an at least partially resolved immune response by activating CD8' memory T cells, CD4 memory T cells, or both.

7 27/11 '06 MON 15:07 [TX/RX NO 57351 27-11-' 6 14:05 FROM-DCC SYDNEY +61292621080 T--90 P017/046 F-074 In still other embodiments, an IRM compound may be administered in one or more separate booster doses after administration of the antigen-IRM combination. The IRM compound provided in a booster dose may be the same or different that the IRM compound provided in the antigen-IRM combination, and may be the same or different S than the IRM compound provided in any other booster dose. Moreover, any combination of IRM compounds may be used, whether as the IRM component of an antigen-lRk combination or as a booster.

Many of the IRM compounds are small organic molecule imidazoquinoline amine derivatives (see, U.S. Pat. No. 4,689,338), but a number of other compound classes are known as well (see, U.S. Pat. Nos. 5,446,153; 6,194,425; and 6,110,929) and more are still being discovered Other IRMs have higher molecular weights, such as oligonucleotides, including CpGs (sea, U.S. Pat. No. 6,194,388).

Certain KIMs are small organic molecules molecular weight under about 1000 Daltons, preferably under about 500 Daltons, as opposed to large biological molecules such as proteins, peptides, and the like) such as those disclosed in, for example, U.S. Patent Nos. 4,68338; 49,8; 4, ,624; 5,266,575; 5,268,376; 5,346,905; 5,352,784; 5,389,640; 5,446,153; 5,482,936; 5,756,747; 6,110,929; 6,194,425; 6,331,539; 6,376,669; 6,451,810; 6,525,064; 6,541,485; 6,545,016; 6,545,017; 6,573,273; 6,656,938; 6,660,735; 6,660,747; 6, 6,66,26;6,664,264; 6,664,265; 6,667,312; 6,670,372; 6,677,347; 6,677,348; 6,677,349; 6,683,088; 6,756,382; 6,797,718; and 6,818,650; U.S. Patent Publication Nos, 2004/0091491; 2004/0147543; arid 2004/0176367; and International Publication Nos, WO 2005/18551, WO 2005/18556, and WO 2005/20999.

.Additional examples of small molecule IRMs include certain purine derivatives (such as those described in U.S. Patent Nos. 6,376,501, and 6,028,076), certain imidazoquinoline amide derivatives (such as those described in U.S. Patent No.

6,069,149), certain irnidazopyridine derivatives (such as those described in U.S. Patent No. 6,518,265), certain benzimidazole derivatives (such as those described in U.S. Patent 6,387,938), certain derivatives of a 4-aminopyrimidine fused to a five membered nitrogen containing heterocyclic ring (such as adenine derivatives described in U. S. Patent Nos.

6,376,501; 6,028,076 and 6,329,381; and in WO 02/08905), and certain 3-p-Dibofranosylthia2olo[4,5-d]pyrimidine derivatives (such as those described in U.S.

Publication No. 2003/0199461).

8 27/11 '06 MON 15:07 [TX/RX NO 5735] 27-11-' 06 14: 05 FROM-DCC SYDNEY +61292621080 T-090 P018/046 F-074 Other IRMs include large biological molecules such as oligonuclectide sequences, Some IRMt oligonucleotide sequences contain cytosine-guanine dinucleotides (CpG) and are described; for example, in U-S. Patent Nos. 6,194,388; 6,207,646; 6,239,116; 6,339,068; and 6,406,705 Some CpG-containing oligonucleotides can include synthetic immunormnodulatory structural motifs such as those described, for example, in US. Patent Nos, 6,426,334 and 6,476,000. Other IRM nucleotide sequences lack CpG sequences and are described, for example, in International Patent Publication No. WO 00/75304 Other IRMs include biological molecules such as aminoalkyl glucosarinide phosphates (AGPs) and are described, for example, in U.S. Patent Nos. 6,113,918; 6,303,347; 6,525,028; and 6,649,172.

IRM compounds suitable for use in the invention include compounds having a 2aminopyfdine fused to a five membered nitrogen-containing heterocyclic ring. Such compounds include, for example, imidazoquinoline amines including but not limited to substituted irnidazoquinoline amines such as, for example, amide substituted irnidazoqinoine amines, sulfonamide substituted imidazoquinoline anrines, urea substituted imidazoquinoline aines, aryl ether substituted imidazoquinoline amines, heterocyclic ether substituted inridazoquinoline amines, amido ether substituted imidazoquinoline amintes, sulfonamido ether substituted imidazoquinoline aimines, urea substituted imidazoquinoline ethers, thioether substituted imidazoquinoline amines, hydroxylamine substituted imidazoquinoline arinies, oxime substituted imidazoquinoline amines, or 9--arvl, heteroaryl, aryloxy or arylalkyleneoxy substituted imidazoquinohiue amines, and iranidazoquinoline diamines; tetrahydroitnidazoquinoline anines including but not limited to arnide substituted tetrahtydroimidazoquinoline amnines, sulfonamide substituted tetrahydroirnidazoquinoline amines, urea sbstituted tetrahydroimidazoquinoline amines, aryl ether substituted tetrahydroimidazoquinoline antines, heterocyclic ether substituted tetrahydrointidazoquinoline amines, arnido ether substituted tetrahydroimidazoquinoline amines, sulfonamido ether substituted tetrah-ydroimiiidazoquinoline amtines, urea substituted tetrahydroinidazoquininoline ethers, thiether substituted tetraiihydroirnidazoquinoline amines, hydroxylarnine substituted tetrahydroimidazoquinoline amines, oxime substituted tetrahydroimidazoquinoline amines, and tetrahydroimidazoquinoline diamines; imrnidazopyridine amines including but not limited to amnide substituted imnidazopyridine amines, sulfonamide substituted 9 27/11i '06 MON 15:07 [TX/RX NO 5735] 27-11-06 14: 06 PEON--DOGC SYDNEY +61292621080 T-090 P019/0416 F-074 imidazopyi-idine arnus, urea substituted imidazopyridine amines, aryl ether substituted imnidazopyridinem anines, heterocyclic ether substituted imidazopyridine amines, amido ether substituted irnidazopyridine amines, sul~fonamdo ether substituted imidazopyri"dine amrines, urea substituted imidazopyridine ethers, and thicether substituted irnidazopyridine amines; I ,2-bridged imr~idazoquinoline amines; 6,7-fused cv-cioalkylimidazopvridine amnines Iidazonaphthyridine amines; tetrahydroixnidazonaphthyridine amin es; oxazoloquinolie amines, thiazoloquinolinie amuies; oxazolopyridifie arnines; rhiazolopyridine aminies; oxazolonaphthyridine aines; thiazolonaplithyridine amines; mvrazoiopyridine amines; pyrazoloquinoline amines; tetrahyi dropyraz-oloqninoli.ne attunes;' I pyrazolonaphthyi.dinme amines; tetrahydropyrazoionaphthyridin pni~;adI-mdz dirners fbsed to pyridine- amines, quinoline amines,, tetr-ahydroquinoline ,arines, naphhyrdineamiesor tetrahydronaptithyridine amines.

In certain embodiments, the IRMf compound may be an imidazonaphithyridirie amine, a tetrahyd-roimiidazoniaphthy)ridine a-mine, an oxazoloquinoline aniirie, a thiazoloquinolie amine, an oxazolopyridine ain-e. a thiazolopyridine anme, ani oxzoonaphithyridine amine, a thiazoilonapbthyridine amine.) a pyrazolopyndmne amie, a pyrazoloquinloline amnine, a tetrahydropyrazoloquinoline amine, a pyrazolonaaphthyridine amine, or a ttavrprzinptyiieaie In certain embodiments., the ERM compound miay be a substituted imidazoquin"7olirei amne, a tetrahydroimidazoquinoline amtne, an iinidazopyridinec amine, a 1,2-Inndged Tuidazoquinoline aminue, a 6,7-fused cveloalkylirnidazopyridine amine, an Pnidazonaphthyridie amine, a tetrahiydroi-ildazonaph thyridine amine, anA oxazolloquinoline amine, a thiazoloquinoine amine, an oxazolopyridine amiune, a thiazolopyridine amine, an oxazolonaphthyridine amaine, a thilazoilonap~hthy-ridine ,amiine, a pyrazolopyridine aminie, a pyrazoloquino 1 ine amine, a trayo rzooinlneamine, a pyr azoionaphthyridine aine, or a tetrahydropyr-aa-olonaphthysidine amine.

As used herein, a substituted imi dazoqumnoline amine refers to an aidde substituted imnidazoquinioline amnine, a sulfonamide substitutedii idazoquinoline amnine, a urea substituted imidazoqinol l'ie amnine, anE axyl etherT substituted imidazoquinkoline amne,aheterocyclic ether substituted iinidazoquinoline amine, an amido ether substituted imidazoquinoline azine, a suifonamnido ether substituted iinidazoquinolino amine, a urela s ubstituted imidazoquin-oline ether', a thioether substit-uted iinidazoquinohnie amine, a 27/11 '06 MON 15:07 VTX!RX NO 57351 2-1- 06 14: 06 PEON"T-DOG SYDNEY +6M92621080 T090 P020/046 FE074 hydroxylaminie substituted imidazoquinoline amine, an oxine substituted hnjidazoquinoiine amline, a or 2-aryt heteroaryl, aryloxy or arylalkyleneoxy substituted imidazoquinoline amine, or an inidazoqinoline diamine. As used herein, substituted imidazoquinoline amin es specifically and expressly exclude 1-(2rnethylpropyi> IH1-imindazo[4,5c]quinolin-4-amine and 4-xmino-a,a-dxnethwl-2e thoxvmethyM -Rimidazo[4,$-c]quino' in.-i-ethanol.

Suitable ThA4l compounds also may include the purine derivatives, I midazoquinoline amide derivYatives, benzixuidazole derivatives, adenine derivatives, aminoallkyl glucosaminide phosphates, and oligonucleotide sequences described above.

In certain embodiments, the MIT' compound may be an arnide substituted imnidazoquinolin amine siich as, fobr example, i-(2-amnino-2-mneth-ylpropyi)-2- (ethxymthyi- lI-inidzo[45-cqniolin4-aineor 2-[4-8a, ino-2- (ethoxymethyl)- 1H-irnidazo [4.,5-c]quiuolin- I-yll I 1 -dimethylthl aI o-6-oxohexyl]- 4-a2zido-2-hydroxybenza.rnide.

In other emboaiments, the HIM compound may be a thiazoloquinoli-ine aminle such as, for example, 2-bultylthiazolo[4,5-cjquinoln--4-am.aine.

In other embodiments, the 1PM compouind mnay be an imidazoquinoline amine such a s, for example,4-aio-eaimhy-2thxmty-IRiiaz[45-cquniv other embodiments, the IRPM compound m ay be an armide substituated imidazoquin~oline amine such as, for example, N- (3-44-amino- 14-2-Methyipxo~pyl)-I.Hixnidazo[-45-cjquinioin--7--vloxyjpropyl) nicotlinamide.

Ini other embodiments, the 1KM compound may be a sulfonamide substituted imidazoquinoline amine such as, for example,3-4ai--(toyeyliZI iizo4S-]uioi- yJN22-terypoae1-sulfonamide.

In other erabodiments, the 1PM compound may be a thioether substituted imidazoquinoline amine such as, for e-xample, 2-butyl-i-42-methyl-2-[2- (methylsuifonyvl)e-thoxy]propyi}- li other emnbodiments, the 1P.M compound may be a pyrazoloquinolsine amine such as., for examrple, 2-hulyl- I -[2-(propyliulfonyl)ethyvi)-2H-pycTazolo[3,4-clqu.inoii-4-amme4.i 27/1i '06 MON 15:07 [TX/RX INO 57351 27-11-' 06 14: 06 FROM-DCC SYDNEY +61292621080 T-090 P021/046 F-074 In other embodiments, the IRM compound may be an arylalkyleneoxy substituted imidazoquinoline amine such as, for example, 1- {4-amino-2-ethoxymethyl-7-[3-(pyridin- 3-yl)propoxy]1--H-imidazo[4,5-c]quinolin-I -yl) -2-ntethylpropan-2-ol.

In other embodiments, the IRM compound may be a urea substituted imidazopyridine amine such as, for example, N- l-mino-2-(etoxcyethyl)-6,7dimethyl-IH-imidao2[4,5-c]pyridin- 1-yl}-1, I -diethylethyl} -N'-cyclohexybrTea.

In other embodiments, the IRM compound may be a sulfonamide substituted imidazoquinoline amine such as, for example, N-[2-(4-amino-2-butyl- I c~quinolin- I 1,1 -dimethylethyl]methanesulfonamide.

In still other emibodiments, the IRM compound may be an amnide substituted imidazoquinoline amine such as, for example, N- {2-[4-amino-2-(ethoxymethyl)-iH- -c]quinolin-l-ylI]-1,1 -dimethylethyl) cyclohexanecarboxamide.

Unless otherwise indicated, reference to a compound can include the compound in any pharmaceutically acceptable ffom, including any isomer (e diastereomer or enantiomer), salt, solvate, polymorph, and the like In particular, if a compound is optically active, reference to the compound can include each of the compound's enantiomers as well as racetic mixtures of the enantiorners.

In some embodiments of the present invention, the IRM compound may be an agonist of at least one TLR, preferably an agonist of TLR6, TLR7, or TLR8. In certain embodiments, the IRM compound may be a TLR8-selective agonist. Ir other embodiments, the IRkM compound may be a TLR7-selective agonist As used herein, the term "TLR.8-selective agonist" refers to any compound that acts as an agonist of TLR8, but does not act as an agonist of TLR7. A "TLR7-selective agonist" refers to a compound that acts as an agonist of TLR7, but does not act as an agonist of TLR8. A "TLR7/8 agonist" refers to a compound that acts as an agonist of both TLR7 and TLR8.

A TLRS-selective agonist or a T LR7-selective agonist may act as an agonist for the indicated TLR and one or more of TLRI, TLR2, TLR3, TLR4, TLRLRS, TLR6 TL, R9, or TLRI 0. Accordingly, while "TLR8-selective agonist" may refer to a compound that acts as an agonist for TLR8 and for no other TLR, it may altematively refer to a compound that acts as an agonist of TLR8 and, for example, TLR6. Similarly, "TLR7-selective agonist" may refer to a compound that acts as an agonist for TLR7 and for no other tLR, but it 12 27/11 '06 MON 15:07 ETX/RX NO 57351 27-11-'06 14:06 FROM-DCC SYDNEY *61292621080 T-090 P022/046 F-074 may alternatively refer to a compound that acts as an agonist of T'R7 and, for example, TLR6.

The TLR. agonism for a particular compound may be assessed in any suitable manner. For example, assays for detecting TLR agonism of test compounds are described, for example, in U.S. Patent Publication No. US2004/0132079, and recombinant cell lines suitable for use in such assays are described, for example, in International Patent Publication No. WO 04/053057.

Regardless of the particular assay employed, a compound can be identified as an agonist of a particular TLR if performing the assay with a compound results in at least a threshold increase of some biological activity mediated by the particular TLR.

Conversely, a compound may be identified as not acting as an agonist of a specified TLR.

if, when used to perform an assay designed to detect biological activity mediated by the specified TLR, the compound fails to elicit a threshold increase in the biological activity.

Unless othervise indicated, an increase in biological activity refers to an increase in the same biological activity over that observed in an appropriate control. An assay may or may not be perforned in conjunction with the appropriate control. With experience, one skilled in the art may develop sufficient familiarity with a particular assay the range of values observed in an appropriate control under specific assay conditions) that performing a control may not always be necessary to determine the TLR agonism of a compound in a particular assay.

The precise threshold increase of TLR.-mediated biological activity for determining whether a particular compound is or is not an agonist of a particular TLR in a given assay may vary according to factors known in the art including but not limited to the biological activity observed as the endpoint of the assay, the method used to measure or detect the endpoint of the assay, the signal-to-noise ratio of the assay, the precision of the assay, and whether the same assay is being used to determine the agonrism of a compound for both TLRs. Accordingly it is not practical to set forth generally the threshold increase of TLRmediated biological activity required to identify a compound as being an agonist or a nonagonist of a particular TLR for all possible assays. Those of ordinary skill in the art, however, can readily determine the appropriate threshold with due consideration of such factors.

13 27/11 '06 MON 15:07 [TX/RX NO 57351 27-11-' 06 14:86 FROM-DCC SYDNEY +61292621080 T-090 P023/046 F-074 Assays employing HEK293 cells transfected with an expressible TLR structural gene may use a threshold of, for example, at least a three-fold increase in a TLR-mediated biological activity NTFKB activation) when the compound is provided at a concentration of, for example, from about I pM to about 10 IiM for identifying a compound as an agonist of the TLR transfected into the cell. However, different thresholds and/or different concentration ranges may be suitable in certain circumstances.

Also, different thresholds may be appropriate for different assays.

A component of an antigen-IRM combination, as well as an antigen or IRIM provided in a priming dose or booster dose, may be provided in any formulation suitable for mucosal administration to a subject. Suitable types of formulations are described, for example, in U.S. Pat. No. 5,939,090; U.S. Pat. No. 6,365,166; U.S. Pat. No. 6,245,776; and U.S. Pat. No. 6,486,168. The compound whether antigen or IRM compound- may be provided in any suitable form including but not limited to a solution, a suspension, an emulsion, or any form of mixture. The compound may be delivered in formulation with any pharmaceutically acceptable excipient, carrier, or vehicle. Moreover, the IRM component and antigen component of an antigen-IRM combination may be provided together in a single formulation or may be provided in separate formulations. A formulation may be delivered in any suitable dosage form such as, for example, a cream, an3 ointment, n a erosol formulation, a non-aerosol spray, a gel, a lotion, and the like. The formulation may further include one or more additives including but not limited to adjuvants, penetration enhancers, colorants, fragrances, flavorings, moisturizers, thickeners, and the like.

A formulation may be administered to any suitable mucosal surface of a subject such as, for example, oral, nasal, or urogenital mucosa.

The composition of a formulation suitable for mucosal vaccination will vary according to factors known in the art including but not limited to the physical and chemical nature of the component(s) the IRM compound and/or antigen), the nature of the carrier, the intended dosing regimen, the state of the subject's immune system (e.gg, suppressed, compromised, stimulated), the method of administering the component(s), and the species to which the formulation is being administered. Accordingly, it is not practical to set forth generally the composition of a formulation effective for mucosal vaccination 14 27/11 '06 MON 15:07 [TX/RX NO 5735] 27-11-' 06 14:07 FROM-DCC SYDNEY +61292621080 T-0890 P24/046 F-074 for all possible applications. Those of ordinary skill in the art, however, can readily determine an appropriate formulation with due consideration of such factors.

In some embodiments, the methods of the present invention include administering IRM to a subject in a formulation of, for example, from about 0.0001% to about (unless otherwise indicated, all percentages provided herein are weight/weight with respect to the total formulation) to the subject, although in some embodiments the IRM compound may be administered using a formulation that provides 1 M compound in a concentration outside of this range. In certain embodiments, the method includes administering to a subject a formulation that includes at least about 0.01% IRM compound, at least about 0.03% IRM compound, or at least about 0.1% IRM compound.

In other embodiments, the method includes administering to a subject a formulation that includes up to about 5% IRM compound, up to about 1% IRM compound, or up to about IRM compound. In one particular embodiment, the method includes administering the IRM compound in a formulation that includes from at least about 0.1% IRM compound up to about 5% IRjM compound.

In some embodiments, a formulation may be administered to the mucosal surface that is a typical or expected site of infection by a particular pathogen. For example, a mucosal vaccine, or a component of a mucosal vaccine may be administered to the nasal mucosa in order to vaccinate against a respiratory pathogen (e an influenza virus) Alternatively, a formulation may be administered to one mucosal surface in order to induce an immune response at a distant nucosal site. For example, a formulation may be administered to the nasal mucosa or oral mucosa in order to vaccinate against a pathogen that can infect through, for example, the vaginal mucosa a herpesvirus).

An amount o aan IRM compound effective for mucosal vaccination is an amount sufficient to increase an irrnune response to the antigen in the combination compared to the immune response raised by administering the antigen without the IRM compound.

The precise amount of IRM compound administered in a mucosal vaccine will vary according to factors known in the art including but not limited to the physical and chemical nature of the IRM compound, the nature of the carer, the intended dosing regimen, the state of the subject's immune system suppressed, compromised, stimulated), the method of administering the 1RM compound, and the species to which the mucosal vaccine is being administered. Accordingly, it is not practical to set forth 27/11 '06 MON 15:07 [TX/RX NO 5735] 27-11-'06 14:07 FROM-DCC SYDNEY +61292621080 T-090 P025/046 F-074 generally the amount that constitutes an amount of IRM compound effective for mucosal vaccination for all possible applications. Those of ordinary skil in the art, however, can readily determine the appropriate amount with due consideration of such factors.

In some embodiments, the methods of the present invention include administering sufficient IRM corpound to provide a dose of, for example, from about 100 ng/kg to about 50 mg/kg to the subject, although in some embodiments the methods may be performed by administering IRM compound in a dose outside this range. In some of these embodiments, the method includes administering sufficient IRM compound to provide a dose of from about 10 lig/kg to about 5 mg/kg to the subject, for example, a dose of about 3.75 mg/kg.

The dosing regimen may depend at least in part on many factors known in the art including but not limited to the physical and chemical nature of the IRM compound, the nature of the carrier, the amount of IRM being administered, the state of the subject's immune system suppressed, compromised, stimulated), the method of administering the iRM compound, and the species to which the mucosal vaccine is being administered.

Accordingly it is not practical to set forth generally the dosing regimen effective for mucosal vaccination for all possible applications. Those of ordinary skill in the art, however, can readily determine an appropriate dosing regimen with due consideration of such factors.

In some embodiments, the IRM compound may be administered, for example, from once to multiple doses within a set time period daily, per week, etc.). In certain embodiments, the IRM compound may be administered a single time. In other embodiments, the IRM may be administered from about once every ten years to multiple times per day. For example, the IRM compound may be administered at least once every ten years, at least once every five years, or at least once every two years. In other embodiments, the IRM compound may be administered, for example, at least once per year, at least once every six months, at least once per month, at least once per week, or at least once per day. In one particular embodiment, the IRM compound is administered from about once per month to about once per year.

The methods of the present invention may be performed on any suitable subject Suitable subjects include but are not limited to animals such as but not limited to humans, non-human primates, rodents, dogs, cats, horses, pigs, sheep, goats, or cows.

16 27/11 '06 MON 15:07 [TX/RX NO 5735] 27-11-106 14:07 PEOM-DOG SVIDNEY 62620 T9 P6/41>7 +6129'2621080 'F-090 P0261/046 F-074 Examples The followin examples ha-ve been selected merely to frrther illustrate features, advantages, anid other- details Of the finvention. It is to be expressly understood, however, that while the examples sere this purpose, the particular materials arid amounts Used as well as other conditions, arid details ar-e niot to be construed in a matter that would unduly limit the scope of thils invenition.

The IRM- compounds used in the examples are shown in Table I.

Table I £nmound jChemical Name Reference IRIM {2-[4-ami-no-2-(ethoxymethyl)- iN- US, 2004/00914-91 dimethylethyl) amino)-6-oxohexyfl-4-azido.

2-hydroxybeuaiaide WMN-2 1-(2-aminio.2-methylpropy1)-2- U.S& 6,677,349 (ethoxymethyl)-- iH-imidazo[4,5-c]quinoim Exa-mple 164, Part I _______4-amIxun IRM3 2-butyithiiazolo[4,5-c]quiniolin--4-amrinie U&S 6,110,929

IS

RN44 ~4-ammio-ctia-dimetyl-2-eth~xyethylIHt US. 5,389,640 ____imidazo unlia5-tanl-__ Ex ample 99 IMS N- 3)-[4-aminlo- I <2-methylpropy1> Lu- U.S. Sern No.

imi'dazo[4,5-cquinoin7 60/508634 vloyj _pl.)nicofinamide Examle 16 IRTVI6 3-1[--4-arinio-2-(ethioxytnethiyl)- IH- PCT App. -No.

I-ylJ-N,2>2 US04/43447 _________triniethyipropasue- 1 -sulfonanmide Exam-Ple 36 IRM7 2butyl- I-{f2-methyl-2-['2- PCT App. No.

(,-nethylsultbny1)ethoxy~rpi N USO4/403 83 uidazo[4,5.-~unln4ann Example 32 M8 2-butyl-i1.-[24rpopys-ulfoniy)e hyl]2TH- PC? App. Noe pyr-azoio[3,4..clquinoliLn-4-aaiiine U S) 0 48 I~ 34aio2-toy Example 122 L r eh ir l_ f RMI 0 ~N{-4aio2(tovch}+US. 6,545,07' N-[2-(4-am ino&2-butyl-IH-imidazo[4,5 U.67739 27/11 '06 MON 15:07 [TX/RX NO 5735] 27-11-' 06 14:07 FROM-DCC SYDNEY +61292621080 T-090 P027/046 F-074 Comrpoud Chemical Name Reference ________dimethylethyljrmethanesulfonamide t IRM12 N- {2-[4-ami no-2-(ethoxymethyl U.S. 6,756,38Z' imidazo(4,5-c]quinolin-1-yl-l, 1dimrnethylethyl cyclohexanecarboxanmide This compound is not specifically exemplified but can be readily prepared using the synthetic methods disclosed in the cited reference.

Example 1 S Ana Ovalburin-IRMI1 conjugate was prepared as follows. AIi1 was suspended in dimethyl sulfoxide (DMSO) to 10 mrg/m. Ovalburnin was suspended in phosphate buffered saline (PBS) to 10 mg/m! and the pH adjusted to 10.0 by the addition of NaOH.

500 L of the ovalbumin solution (5 mg ovaibunin) was mixed with 100 pL of the IRM1i solution (1 mg IRMI) in a single well of a 12-well tissue culture plate. The plate was placed on ice and a long wavelength UV light source was placed directly over the plate as close to the well containing the IRMI/ovalbunmin mixture as possible, The mixture was irradiated for 15 minutes. The resulting conjugate was removed from the well and resuspended in PBS to a final concentration of5 mg/mhLt ovalbumin, 0.5 mg'm. MIRMI, and dialyzed against PBS to remove any unconjugated IRLM.

Chicken Ovalbumin-specific CD8 T cells (OT-I, The Jackson Laboratories, Bar Harbor, ME) were labeled with carboxyfluoroscein succinimidyl ester (CSE, Molecular Probes, Inc., Eugene, OR), a fluorescent dye that stains cells in a stabile maimer, and then adoptively transferred into syngenic C57B116 mice (Charles River Laboratories, Wilmington, MA). The recipient mice were then immun ized on Day 0 with 100 micrograms (pg) of the Ovalbumin-IRii conjugate, either intranasally (IN) or intravenously On Day 4, the mice were sacrificed and the nasal associated Jymphoid tissue (NALT), inguinal lymph nodes (ILN), cervical lymph nodes (CLN), and spleens (Spl) were removed. Each tissue harvested from the mice was uin thaough a 100 pm nylon screen (BD Biosciences, Bedford, MA), centrifuged, and resuspended in Flow Cytometry Staining Buffer (Biosource International, Inc., Rockviile, MD). Cells were then labeled with CD8-cychrome (BD Pharmigen, San Diego, CA) and SIINFEKL/Kb tetramer-phycoerytherine (Becbmnan Coulter, Inc., Fullerton, CA) antibodies. Cells were then run on a FACSCaliber (Becton, Dickinson, and Co., San Jose, CA) and CD8* SIINFEKL/K tetramerf T cells were analyzed for CESE expression.

18 27/11 '06 MON 15:07 [TX/RX NO 57351 27-11-'06 14:07 FROM-DCC SYDNEY +61292621080 T-090 P028/046 F-074 Results axe shown in Figure 1 as follows: NALT in Figure IA; rLN in Figure 1B; CLN in Figure 1 C; and Spleen in Figure ID. Intranasal delivery of antigen with IRM results in the effective activation of cytotoxic T lymphocytes in all locations, as indicated by a progressive loss of CFSE, Separately, total OT--I cell numbers at Day 7 were counted in nasal associated lymphoid tissue (NALT), cervical lymph node (CLN), and spleen (Spl). OT-I cell numbers were determined by counting total lymphocytes (Trypan blue exclusion) and multiplying by the percentage of OT-tICD8s (flow cytometry analysis). Additionally, the percentage of OT--I cells mi the nasal mucosa at determined at Day 7. Results are shown in Figure 2 as follows: NALT in Figure 2A; in Fin Figure 2B; Spleen in Figure 2C; and nasal mucosa in Figure 2D.

Intranasal delivery of antigen plus IRM1 generated greater total OT-I cell numbers at Day 7 than intravenous delivery in all lymphoid tissues examined. Intranasal delivery of IRM1 plus antigen also generated greater total OT- cell numbers at Day 7 than antigen alone, indicating a dramatic effect of the IRNM in enhancing antigen specific T cell activation via that route. Furthermore, the intranasal route of vaccination results in a greater number of OT-I cells at relevant tissue sites the nasal associated lymphoid tissue (NALT) and the nasal mucosa.

Example 2 CD8' T cells from OT-I mice (The Jackson Laboratories, Bar Harbor, ME) were adoptively transferred into C57BL/6 (Charles River Laboratories, Wilmington, MA) mice.

CD4" T cells from DO. 11 TCR mice (The Jackson Laboratories, Bar Harbor, ME) were adoptively transferred into Balb/c mice (Charles River Laboratories, Wilrington, MA).

The mice were then immunized intranasally at Day 0 as follows: OT-I-transferred C57BL/6 mice were immunized with 100 p.g whole chicken ovalbumin per mouse, either with (IRM2 Ag, 75 ug IRM2/mouse) or without (Ag alone) IRM2; DO. 11-transferred Balb/c mice were immunized with 100 gg OVA peptide (ISQAVHAAH-AEINEAGR) per mouse, either with (IRM2 Ag, 75 pg IRM2/nouse) or without (Ag alone) IRM2.

On Day 3, the nasal associated lymphoid tissue was removed and the fold expansion of each cell population over PBS alone was determined. CD' OT--I cells were detected using SIINFEKL/'Kb tetramers and CD4 1 DO. 11 cells were detected using a 19 27/11 '06 MON 15:07 [TX/RX NO 5735] 27-11--'06 14: 08 FOM-DCC SYDNEY 461292621080 T-090 P029/046 F-074 clonotypic antibody (Caltag Laboratories, Burlingame, CA) and analyzed using a FACSCaliber (Becton, Dickinson, San Jose, CA), Results are shown in Figure 3 as follows: CD8' OT--I expansion is shown in Figure 3A; CD4+ DO. I expansion is shown in Figure 3B. Intranasal inmununization of an TIhM/antigen combination induces expansion of both CD8 t T cells and C0D4' 4 T cells to a greater extent than intranasal imnunization with antigen alone, Example 3 Balb/c mice (Charles River Laboratories, Wilraington, MA) were treated with ug of whole chicken ovalburini (OVA) protein (Sigma-Aldrich, St.Louis, MO) with 50 tg of IRM4 in phosphate buffered saline (PBS) by various routes Clean ovalbumin protein was prepared by washing the OVA with Bio-Beads (Bio--Rad Laboratories, Inc., Hercules, CA, Cat# 152-3920) to remove endotoxin, then resuspended in phosphate buffered saline (PBS). Mice were treated with OVA and IRM4 by sub-cutaneous (SC) injection, intravenous (IV) injection, intra-muscular injection, intra-dernal (ID) injection, intranasal insillation intradermal OVA injection with topical administration of 10 j4L of IRM4 cream directly over the OVA injection site (ID Top.), or were left untreated (nothing).

On day 21, mice were sacrificed, lung and nasal lavages were perfor ed by trachea adminirstration of 1 amL of PBS and serum was obtained by cardiac puncture and centrifugation to remove cells- Serum was collected for analysis. Lavage samples were measured for OVA-specific IgA by ELISA. Serumn samples were measured for OVAspecific IgG2a by ELISA.

The OVA specific antibody ELISAs were preformed by coating Costar EIRIA 96 well plates (Cat#3590, Corning, Inc., Coming, NY) with 100 pL/well of a 20 pg/mL ovalburnin solution in PBS and incubated for one to two hours at 37C or overnight at 41C. Plates were then washed cne time with 0.5% Tween-20 in PBS solution (wash buffer). 200 4L/well of a 1% BSA in PBS solution were placed into the wells, and incubated fbr one to two hours at 37 0 C or overnight at 4"C. Plates were then washed two times with wash buffr. Three-fold serial dilutions starting with undilted lavage samples, or twenty-fold serial dilutions starting with a 1:10 dilution of serum samples were made across the plate in 0.2% BSA, 0.05% Tween-20 in PBS (dilution buffer) and incubated overnight at 40C. Plates were then washed four times with wash buffer. 100 IL/well of a 27/11 '06 MON 15:07 [TX/RX NO 57351 27-11-' 06 14:08 FHOM-DCC SYDNEY +61292621080 T-090 P030/046 F-074 1:d2000 dilution of goat anti-mouse IgG2a (Southern Biotechnology Associates, Inc., Binrmingham, AL) or goat anti-mouse IgA (Southern Biotechnology Associates, Inc.) in dilution buffer was placed into the wells and incubated at room temperature for one hour.

Plates were then washed four times with wash buffer, filled with 100 iL/well of stabilized chromagen (Cat#SB02, Biosource International, Camarillo, CA), incubated for less than five minutes, and 50 jiL/well of stop solution (Cat#SSO2, Biosource International) were then added. Plates are read on a spectrophotometer at an OD of 490 The results are shown in Fig. 4. Only intranasal administration of the IRM/antigen combination generated strong IgA responses in the lung (Fig. 4A) and nasal (Fig. 43) mucosa. All routes of administration, including intranasal, generated strong IgG2a responses in the blood (Fig, 4C).

Example 4 On Day 0 and Day 7 Balb/c mice (Charles Rivers Laboratories) were immunized intranasally with 35 ig of OVA alone or in combination with 14 jIg of IRM3, fRM4, iRMS, IIRM6, IRM7, IRM8, IRM9, 1RM10, IRM1 411, or I1RM12 in PBS. On Day 14, mice were sacrificed and lung lavage and serum collection was performed as described in Example 3. Lung lavage and serum samples were analyzed for OVA specific IgA and igG2b (Southern Biotechnology Associates, Inc.), respectively, as described in Example 3.

The results are shown in Fig. 5. IRM/autigen combinations of all IRM compounds tested provided greater IgA (Fig. 5A) and IgG2b (Fig. 5B) responses than antigen alone.

Exanple Lymphocytes from lymph nodes of GFP+/OT-I+ CS7BL6 mice were adoptively transferred into C57BL6 maice. One dayafter adoptive transfer, the mice were immunized nasally with 35 jig of ovalbumin alone or in combination with 14 pig of IRM3, IRM4, M6N, 1RM7, IRMS, RM, IRMI0, IRMI or IRMI2 in citrate buffered saline (CBS). Four days later, mice were sacrificed and draining lymph nodes (DLN) and spleens were removed. The total number of DLN lymphocytes and splenocytes were deternined by using a Guava PCA 96 (Guava Technologies, Inc., Hayward, CA) DLN lymphocytes and splenocytes were stained with propidium iodine (PI) and mouse anti- CD8 antibody (BD Pharmingen, San Diego, CA) and the percentage of OT&-/GF' 21 27/11 '06 MON 15:07 [TX/RX NO 57351 27-11-'06 14: 08 FOM--DCC SYDNEY +61292621080 T-090 F031/046 F-074 lymphocytes was determined by flow cytometry gating on PFCD8/GFP' iymiphocytes.

The total number of OT-'GFP t lymphocytes was determined by multiplying the total number of splenocytes by the percent P.TOTA-GFP t I lymphocytes.

The results are shown in Fig. 6. Intranasal administration of TIvM/antigen combinations employing many different IRM compounds provided greater number of antigen-specific T cells in the DLN (Figs 6A) and the spleen (Figs. 6B) than administration of antigen alone.

Example 6 Lymphocytes from OT-Imice (The Jackson Laboratories, Bar Harbor, ME) were adoptively transferred into C57BL/6 (Charles River Laboratories, Wilmington, MA) mice.

Ovalbumnin was washed as described in Example 3. One day after the adoptive transfer, nice were immunized with PBS alone intranasally or 50 jig of ovalbumin and 50 jig of IRM4 in PBS intranasally intravenously or subcutaneously Five months later, mice were either immunized again in the same manner they had been iramunized previously, or were not re-immunized Mice were sacrificed four days after the five-month iammunization and the draining lymph nodes (DLN) and nasal associated lymphoid tissue (NALT) were collected.

The total number of DLN lymphocytes and NALT lymphocytes were detennined by using a Guava PCA 96 (Guava Technologies, Inc., Hayward, CA).

DLN lymphocytes and NALT lymphocytes were stained with propidium iodine (P) and mouse anti-CD8 antibody (BD Pharmingen, San Diego, CA) and the percent of OT-i/GFP 4 lymphocytes was determined by flow cytomnetry gating on PICD87GFP t lymphocytes. The total number of OT-I/GFP t lymphocytes was determined by multiplying the total number of DLN lymphocytes or NALT lymphocytes by the percent DLN or NALT PIOT-t/FP lymphocytes.

The results are shown in Fig. 7 as follows: DLN in Figure 7A; NALT in Figure 7B. All routes of imnunization, including intranasal, caused an increase in OT-I cell number in the DLN upon re-immunization. Furthennore, intranal immunization caused an increase in OT-I cell number in the NALT upon re-imnummization.

27/11 '06 MON 15:07 ITX/RX NO 5735] 27-11-'06 14: 8 FROM-DCC SYDNEY +61292621080 T-090 P032/046 F-074 The complete disclosures of the patent, patent documents and publications cited herein are incorporated by reference in their entirety as if each were individually incorporated. In case of conflict, the present specification, including definitions, shall control, Various modifications and alterations to this invention will become apparent to those skilled in the art without departing from the scope and spirit of this invention.

Illustrative embodiments and examples are provided as examples only and are not intended to limit the scope of the present invention. The scope of the invention is limited only by the claims set forth as follows.

23 27/11 '06 MON 15:07 [TX/RX NO 57351

Claims (1)

1. 27-11-'06 14:09 FROM-DCC SYDNEY +6129262180 T-090 P033/046 F--074 Wbhat is Claimed is: 1. A pharmaceutical combination comprising: an 1RM compound formulated for mucosal administration; and an antigen formulated for mucosal administration. 2. The pharmaceutical combination of claim I comprising a single formulation that comprises the IRM compound and the antigen. 3. The pharmaceutical combination of claim 1 comprising: a first formulation that comprises the IRM compound; and a second formulation that comprises the antige. 4. A method of immunizing a subject comprising: administering an antigen to a mucosal surface of the subject in an amount effective, in combination with an IRM compound, to generate an immune response against the antigen; and administering an IRM compound to a mucosal surface of the subject in an amount effective, in combination with the antigen, to generate an immune response against the antigen. The method of claim 4 wherein the antigen and IRM are administered in one formuiation 6. The method of claim 4 wherein the antigen is administered in a first formulation and the IRM compound is administered in a second formulation. 24 27/11 '06 MON 15:07 [TX/RX NO 57351 27-11-' 6 14:89 FROM-DCC SYDNEY +61292621080 T--09 P834/946 F--74 7. The method of claim 6 wherein the antigen and the IRM compound are administered at different sites. 8. The method of claim 7 wherein at least one site comprises nasal mucosa. 9. The method of claim 7 wherein at least one site comprises oral mucosa. The method of claim 7 wherein at least one site comprises gastro-intestinal mucosa. 11. The method of claim 7 wherein at least one site comprises urogenital nmcosa. 12. The method of claim 7 wherein the different sites are different mucosal surfaces, 13. The method of claim 6 wherein the IRM compound is administered before the antigen is administered. 14. The method of claim 6 wherein the IRM compound is administered after the antigen is administered. The method of claim 4 wherein the antigen comprises a protein, a peptide, a live or inactivated bacterium, a live or inactivated virus, or any combination thereof 16. The method of claim 4 wherein the IRM compound comprises a 2- aminopyridine fused to a five membered nitrogen-containing heterocyclic ring. 27/11 '06 MON 15:07 [TX/RX NO 5735] 1 0 H S N6 9 2 00/4 27-11-'06 14:09 FR;OM--DCC SYDNEY +61292621080 T-090 P035/046 F-074 17. The method of claim 4 further comprising at least one additional administration of the antigen. 18. The method of claim 4 further comprising at least one additional administration of an IRM compound. 19. The method of claim 18 wherein the IRM compound of the first administration of RM compound is different than the IRM compound of the second administration of IRM compound. The method of claim 4 wherein the immune response against the antigen comprises secretion of igA. 21. The method of claim 4 wherein the immune response against the antigen comprises increasing the number or percentage of antigen-specific T cells in a mucosal tissue. 26 27/11 '06 MON 15:07 [TX/RX NO 57351