AU2005232286A1 - Pyrimidine nucleotide precursors for treatment of systemic inflammation and inflammatory hepatitis - Google Patents

Description

S&FRef: 362901D3

AUSTRALIA

PATENTS ACT 1990 COMPLETE SPECIFICATION FOR A STANDARD PATENT Name and Address of Applicant: Actual Inventor(s): Address for Service: Invention Title: Pro-Neuron, Inc., of 1530 East Jefferson Street, Rockville, Maryland, 20852, United States of America Michael K. Bamat Bradley M. Hiltbrand Reid W. Von Borstel Spruson Ferguson St Martins Tower Level 31 Market Street Sydney NSW 2000 (CCN 3710000177) Pyrimidine nucleotide precursors for treatment of systemic inflammation and inflammatory hepatitis The following statement is a full description of this invention, including the best method of performing it known to me/us:- 5845c o Title of the Invention PYRIMIDINE NUCLEOTIDE PRECURSORS FOR TREATMENT OF SYSTEMIC

INFLAMMATION

00 AND INFLAMMATORy

HEPATITIS

Field of the Invention This invention relates generally to pyrimidine nucleotide precursors including acyl derivatives of cytidine, uridine and orotate, and to the prophylactic and therapeutic uses of these Scompounds. The invention also relates to the administration of these compounds, alone or in combinations, with or without other agents, to animals. These compounds are capable of enhancing resistance of an animal to bacterial endotoxin and other inflammatory stimuli, and inflammatory mediators.

Background of the Invention Sepsis, also referred to as sepsis syndrome, is a consequence of serious infection by bacteria, fungi, or viruses. Sepsis accounts for tens of thousands of deaths in preuror inclAudingR acidriaiEPoATII e rd n or t t a d t h r p y a t c n h r p u i s s o h s co p u d T e i v nt o l o r l t s to t e a m n s r t o l^ the United States every year; it is a leading cause of death of patients in surgical intensive care units.

SSepsis is an inflammatory disorder in which endogenous Z cytokines and other bioactive molecules, produced or released in response to an inflammatory stimulus such as bacterial endotoxin (a component of the cell wall of gram-negative 00 bacteria), cause various symptoms including fever, neutropenia, blood coagulation disorders, hypotension, shock, and organ damage.

SSepsis (or in its more severe form, septic shock), is one example of a broader class of disease called the "Systemic Inflammatory Response Syndrome" (SIRS), which is an organism's reaction to inflammatory stimuli such as endotoxin (which can be present in the bloodstream without bacteremia, e.g. due to leakage of endotoxin from gram-negative bacteria into the circulation from a localized infection or from the intestine); SIRS can also be triggered by gram-positive bacteria, fungi, viruses, and can also be a consequence of autoimmune disorders or administration of therapeutic inflammatory cytokines.

Current treatment of SIRS involves circulatory and respiratory support, but does not directly address improvement of tissue resistance to inflammatory stimuli such as endotoxin, or inflammatory mediators.

Monoclonal antibodies for neutralizing endotoxins or mediators of its physiologic effects are under development.

However, it is expensive or impractical to use antibodies as prophylaxis in susceptible patients, prior to the onset of symptoms of endotoxin poisoning. Moreover, it is difficult to V determine which patients are likely to benefit from antibody treatment, since the time required to culture and identify Sinfectious organisms often exceeds the time limit for Z implementation of effective therapy. Similar problems have been encountered in attempts to use receptor antagonists of specific inflammatory meidators like interleukin-1.

00 C 10 Endotoxin toxicity is in part mediated by endogenous C( cytokines and other bioactive molecules released from V macrophages, Kupffer cells (sessile macrophages in the liver) and other cell types in response to endotoxin. Among the most significant of these mediators are tumor necrosis factor (TNF) and interleukin-1 Others include platelet activating factor (PAF), interleukin-6, and leukotrienes and other arachidonic acid derivatives. Administration of these cytokines or mediators results in symptoms similar to at least some of those elicited by endotoxin. Agents or pathological conditions other than bacterial endotoxin can result in elevated production or activity of (or sensitivity to) TNF or IL-1, resulting in tissue damage. Such conditions include infection with gram-positive bacteria, viruses or fungi, or liver damage. Inflammatory cytokines can produce tissue damage if present in excess, but when elicited in moderate amounts, they are important in the defense against infectious organisms or viruses. For example, antibodies to TNF can reduce toxicity of an administered dose of endotoxin (by blocking the negative effects of TNF elicited by the endotoxin), but can have a deleterious effect in the case of some bacterial infections, converting a sublethal state of infection into an overwhelming lethal infection (Havell, J.

SImmunol., 1987, 139:4225-4231; Echtenacher et al., J.

Immunol., 1990 145:3762-3766). Thus, there are inherent Sproblems with strategies for treating sepsis syndrome or SIRS Z with agents which directly inactivate inflammatory cytokines.

The liver is a major site for clearance or detoxification \0 of endotoxin (Farrar and Corwin, Ann. N.Y. Acad. Sci., 1966 00 C I0 133:668-684) and inflammatory proteins like TNF; conversely, the liver is susceptible to damage by endotoxin and its I mediators. Liver damage from many originating causes (e.g.

O carbon tetrachloride, choline deficiency, viral infection, Reye's syndrome, alcohol) is in part mediated by bacterial endotoxin or mediators elicited by endotoxin even when symptoms of systemic sepsis are not present (Nolan, Gastroenterology, 1975, 69:1346-1356; Nolan, Hepatology, 1989, 10:887-891). Hepatic toxicity is dose-limiting in patients receiving intentional injections of endotoxin for possible efficacy in treating cancer (Engelhardt et al., Cancer Research, 1991, 51:2524-2530). The liver has been reported to be the first vital organ displaying pathological alterations in septic shock (Kang et al., J. Histochem. Cvtochem., 1988 36:665-678). Moreover, hepatic dysfunction occurs in the early stages of sepsis and may initiate sequential organ failure (Wang et al., Arch. Sur., 1991, 126:219-224) The liver is important in regulating the sensitivity of an animal to endotoxin. Various treatments which impair liver function or metabolism, such as poisoning with lead acetate, cycloheximide, Actinomycin D or galactosamine can increase the sensitivity of animals to endotoxin or TNF, in some cases by O several orders of magnitude.

Galactosamine-induced liver damage is unique in that it Z is readily reversible during a period before cell death occurs. Galactosamine selectively depletes hepatic uridine nucleotides, by locking them into UDP-hexosamines that are not 0 converted back into free nucleotides. This can lead to liver 00 damage if the depletion of uridine nucleotides is sufficiently S prolonged, due to impairment of RNA and protein synthesis.

The biochemical deficiency induced by galactosamine is readily C 15 reversed by administration of uridine, which replenishes the uridine nucleotides trapped by the galactosamine. Thus, administration of uridine shortly before or after 0 administration of galactosamine attenuates galactosamineinduced hepatic damage and consequently restores sensitivity to endotoxin toward normal values (Galanos et al., PNAS, 1979, 76:5939-5943).

Similarly, endotoxin hypersensitivity in mice deliberately treated with the rodent hepatotoxin TCDD was partially reversed by administration of uridine (Rosenthal et al., Toxicology, 1989 56:239-251).

However, in contrast to these situations wherein uridine partially reversed experimentally-reduced resistance to endotoxin, uridine was reported to have no protective effect in normal mice challenged with endotoxin (Markley et al., J.

Trauma 1970, 10:598-607), it did not result in greaterthan-normal resistance to endotoxin.

Uridine, cytidine, and orotate have been tested for O effects on liver function in hepatic disorders and in experimental models, with mixed results. Shafer and 0 Isselbacher (Gastroenteroloqy, 1961, 40:782-784) reported that daily intravenous infusion of 25 to 100 milligrams of cytidine and uridine, for 3 to 7 days, to patients with hepatic cirrhosis had no effect on clinical status. Orotic acid added

ID

00 to rat diet in a concentration of 1 percent results in fatty cI C infiltration of the liver (von Euler et al, J. Biol. Chem., 1963, 238:2464-2469); orotic acid administered by Sintraperitoneal injection reduced liver damage in rats treated with carbon tetrachloride, dichloroethane, DDT, and 9,10dimethyl-1.2-benzanthracene (Pates et al., Farmakol Toksikol., 1968, 31:717-719). Lysine-orotate potentiated the toxicity of hepatotoxic extracts from the mushroom Amanita Phalloides; sodium orotate and orotic acid had no effect on Amanita extract toxicity (Halacheva et al., Toxicon, 1988, 26:571-576). Orotic acid has been administered clinically to humans for treatment of neonatal hyperbilirubinemia and for improving recovery from myocardial infarction (O'Sullivan, Aust. N.Z. J. Med., 1973, 3:417-422). Orotate is not well absorbed after oral administration, in part due to poor solubility.

Hata et al. (US Patents 4,027,017 and 4,058,601) disclose that uridinediphosphate and uridinediphosphoglucuronic acid reduce blood alcohol content and inhibit accumulation of the liver after administration of ethanol.

neutral lipids in the liver after administration of ethanol.

Clinical trials involving the administration of uridine for the purpose of attenuating host toxicity of the C-I antineoplastic drug 5-fluorouracil) have been complicated due to the biological properties of uridine itself. Uridine is Z poorly absorbed after oral administration; diarrhea is dose limiting in humans (van Groeningen et al., Proceedings of the AACR, 1987, 28:195). Parenteral administration of uridine OO requires use of a central venous catheter (with consequent C discomfort and risk of infection), since phlebitis was a C problem in early clinical trials when uridine was administered 0 via a brachial venous catheter (van Groeningen et al. Cancer c 15 Treat Rep., 1986, 70:745-50).

Administration of acyl derivatives of uridine and cytidine, which are readily absorbed from the gut into the bloodstream, and which are then hydrolyzed to yield free uridine or cytidine in the circulation, overcome the problem of poor oral absorption of the free nucleosides (US Patent Applications Serial No. 438,493, 115,929, and 903,107, hereby incorporated by reference).

Objects of the Invention It is a primary object of the invention to provide therapeutic and prophylactic agents which are effective in improving survival and in preventing tissue damage from systemic inflammatory response syndrome, including sepsis.

It is a primary object of this invention to provide a family of compounds which effectively enhance resistance to systemic inflammation. Administration of these compounds to an animal before, during or after exposure to endotoxin or 0 other inflammatory stimuli, prevents or treats the effects of CI systemic inflammation.

0 z It is a further object of this invention to provide a family of compounds for the treatment of a variety of disorders involving inflammatory stimuli or inflammatory ND cytokines in their etiology.

00 OC (C CA It is a further object of this invention to provide a family of compounds to improve survival or physiological Sfunctions in animals subjected to endotoxin poisoning or other systemic inflammatory disorders.

It is a further object of the invention to provide a family of compounds to treat or prevent inflammatory hepatitis.

It is a further object of the invention to provide compounds which can be administered orally or parenterally.

Summary Of The Invention These and other objects of the invention are achieved by precursors of pyrimidine nucleotides such as orotic acid or its salts, uridine, cytidine, or prodrug derivatives of these agents including acyl derivatives or phosphate esters, which can be administered to animals, including mammals such as humans. The administration of these compounds alone, or in combination, is useful in treatment or prevention of consequences of systemic inflammation. Systemic inflammation is caused by infection with bacteria, fungi, or viruses, constituents of bacteria, fungi or viruses, endotoxin, polysaccharides or viral proteins Q respectively, by inflammatory mediators, or as a consequence of autoimmune disorders.

SThus, the compounds of the invention, alone or in combination, are useful in the treatment and prevention of sepsis or toxic effects of inflammatory cytokines; are useful as prophylactic agents in patients at risk of sepsis, patients undergoing surgical procedures, or afflicted with serious burns or wounds, or immunocompromised as a consequence of chemotherapy for cancer or other diseases.

An important aspect of this invention is the discovery that pyrimidine nucleotide precursors such as orotate, uridine, or cytidine, and acyl derivatives of such compounds, have unexpected therapeutic 00 properties.

CN ,I One embodiment of the invention involves the use of the compounds and compositions of the rC< invention in treatment and prevention of toxicity encountered during therapeutic administration of Sinflammatory cytokines, for treatment of cancer.

N One embodiment of the invention involves the use of the compounds and composition of the invention in treatment and prevention of inflammatory hepatitis.

According to a first embodiment of the invention, there is provided a composition comprising a parenteral nutrition formula and (ii) 2 to 40 grams of a pyrimidine nucleotide precursor per daily portion wherein said pyrimidine nucleotide precursor is uridine, cytidine, orotic acid, or an acyl derivative of uridine, cytidine, or orotic acid, or a pharmaceutically acceptable salt thereof.

According to a second embodiment of the invention, there is provided a method of providing nutrition to a mammal receiving nutrition intravenously comprising administering to said mammal a composition comprising 2 to 40 grams of a pyrimidine nucleotide precursor per daily portion According to a third embodiment of the invention, there is provided a composition comprising a pyrimidine nucleotide precursor when used in providing nutrition to a mammal receiving nutrition intravenously, wherein said composition comprises 2-40 grams of a pyrimidine nucleotide precursor per daily portion.

According to a fourth embodiment of the invention, there is provided the use of a composition comprising a pyrimidine nucleotide precursor in the manufacture of a supplement for providing nutrition to a mammal receiving nutrition intravenously, wherein said composition comprises 2-40 grams of a pyrimidine nucleotide precursor per daily portion.

According to a fifth embodiment of the invention, there is provided a composition comprising: a) glucose, and b) a pyrimidine nucleotide precursor, wherein said pyrimidine nucleotide precursor is uridine, cytidine, orotic acid, or an acyl derivative of uridine, cytidine, or orotic acid, or a pharmaceutically acceptable salt thereof, and wherein said pyrimidine nucleotide precursor is present in a concentration of between 1-25% by weight.

(I:\I)ayl.ib\I 13P10848 I 8.doc:gcc According to a sixth embodiment of the invention, there is provided a method of treating a a mammal during or after liver transplantation comprising administering a composition comprising Sglucose and a pyrimidine nucleotide precursor.

According to a seventh embodiment of the invention, there is provided a composition Z 5 comprising glucose and a pyrimidine nucleotide precursor when used in treating a mammal during or O after liver transplantation.

According to an eighth embodiment of the invention, there is provided the use of a composition comprising glucose and a pyrimidine nucleotide precursor in the manufacture of a medicament for

O

C treating a mammal during or after liver transplantation.

to According to a ninth embodiment of the invention, there is provided a method of reducing I inflammatory liver injury in an animal in need of such treatment comprising administering to said animal a therapeutically effective amount of an acyl derivative of uridine, cytidine or orotic acid, or a pharmaceutically acceptable salt thereof.

According to a tenth embodiment of the invention, there is provided an acyl derivative of uridine, cytidine or orotic acid, or a pharmaceutically acceptable salt thereof, when used in reducing inflammatory liver injury in an animal in need of such treatment.

According to an eleventh embodiment of the invention, there is provided use of an acyl derivative of uridine, cytidine or orotic acid, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for reducing inflammatory liver injury in an animal in need of such treatment.

According to a twelfth embodiment of the invention, there is provided a method for treating or preventing cachexia comprising administering to an animal a therapeutically effective amount of uridine, cytidine, orotic acid, or an acyl derivative of uridine, cytidine, or orotic acid, or a pharmaceutically acceptable salt thereof.

According to a thirteenth embodiment of the invention, there is provided uridine, cytidine, orotic acid, or an acyl derivative of uridine, cytidine, or orotic acid, or a pharmaceutically acceptable salt thereof when used in treating or preventing cachexia in an animal in need of said treatment or prevention.

According to a fourteenth embodiment of the invention, there is provided the use of uridine, cytidine, orotic acid, or an acyl derivative of uridine, cytidine, or orotic acid, or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for treating or preventing cachexia in an animal in need of said treatment or prevention.

According to a fifteenth embodiment of the invention, there is provided a method for treating or preventing cachexia in an animal in need of said treatment or prevention, comprising administering to an animal a therapeutically effective amount of an inhibitor of uridine phosphorylase.

fI:\DayLib\LIBFF]084 I8b.doc:gcc 9b According to a sixteenth embodiment of the invention, there is provided an inhibitor of uridine 0 phosphorylase when used in treating or preventing cachexia in an animal in need of said treatment or Sprevention.

According to a seventeenth embodiment of the invention, there is provided the use of an S 5 inhibitor of uridine phosphorylase in the manufacture of a medicament for treating or preventing Scachexia in an animal in need of said treatment or prevention.

00

(N'

[I:\DayLib\LIBFF]08418b.doc:gcc Compounds of the Invention O The compounds useful in enhancing resistance to inflammatory stimuli or inflammatory mediators have the

O

0 following structures: In all cases except where indicated, letters and letters with subscripts symbolizing variable substituents in the

ID

00 chemical structures of the compounds of the invention are C 10 applicable only to the structure immediately preceding the CA description-of the symbol.

S(1) Uridine or an acyl derivative of uridine having 15 the formula: o ON0

R

2 0 OR3 wherein R, R, R, and R, are the same or different and each is hydrogen or an acyl radical of a metabolite, or a pharmaceutically acceptable salt thereof.

Cytidine or an acyl derivative of cytidine having the formula:

NHR

0 N

OR,

R0zO OC, 00 m wherein R, and R, are the same or different and each is hydrogen or an acyl radical of a metabolite or a Spharmaceutically acceptable salt thereof.

An acyl derivative of uridine having the formula: 0

HN

O N

RIO

RzO ORA wherein and R, are the same, or different, and each is hydrogen or an acyl radical of a. an unbranched fatty acid with 5 to 22 carbon atoms, b. an amino acid selected from the group consisting of glycine, the L forms of alanine, valine, leucine, isoleucine, tyrosine, proline, hydroxyproline, serine, threonine, cystine, 12 cysteine, aspartic acid, glutamic acid, arginine, lysine, histidine, carnitine and ornithine, Cq c. a dicarboxylic acid having 3-22 carbon atoms, d. a carboxylic acid selected from one or more of the Z group consisting of glycolic acid, pyruvic acid, lactic acid, enolpyruvic acid, lipoic acid, pantothenic acid, acetoacetic acid, p-aminobenzoic acid, betahydroxybutyric acid, orotic NO acid, and creatine.

An acyl derivative of cytidine having the in formula: 2 NHA RA O OR wherein and R, are the same, or different, and each is hydrogen or an acyl radical of a. an unbranched fatty acid with 5 to 22 carbon atoms, b. an amino acid selected from the group consisting of glycine, the L forms of phenylalanine, alanine, valine, leucine, isoleucine, tyrosine, proline, hydroxyproline, serine, threonine, cystine, cysteine, aspartic acid, glutamic acid, arginine, lysine, histidine carnitine and ornithine, c. a dicarboxylic acid having 3-22 carbon atoms, d. a carboxylic acid selected from one or more of the group consisting of glycolic acid, pyruvic acid, lactic acid, 13 enolpyruvic acid, lipoic acid, pantothenic acid, acetoacetic Sacid, p-aminobenzoic acid, betahydroxybutyric acid, orotic Sacid, and creatine.

O An acyl derivative of uridine having the z formula: 0 00 0 tmn

RO

R

2 0 OR3 wherein at least one of or R, is a hydrocarbyloxycarbonyl moiety containing 2-26 carbon atoms and the remaining R substituents are independently a hydrocarbyloxycarbonyl or hydrocarbylcarbonyl moiety or H or phosphate.

An acyl derivative of cytidine having the formula:

NHR

4 0 N RIO0 wherein at least one of R, or R, is a V hydrocarbyloxycarbonyl moiety containing 2-26 carbon atoms and O the remaining R substituents are independently a hydrocarbyloxycarbonyl or hydrocarbylcarbonyl moiety or H or

O

Z phosphate.

Orotic acid or salts thereof:

ID

co0

HN

Pharmaceutically-acceptable salts of orotic acid include those in which the cationic component of the salt is sodium, potassium, a basic amino acid such as arginine or lysine, methylglucamine, choline, or any other substantially nontoxic water soluble cation with a molecular weight less than about 1000 daltons.

8) Alcohol-substituted orotate derivatives:

COOH

02 1 11

COOR,

wherein R, is a radical of an alcohol containing 1 to 20 carbon 0 atoms joined to orotate via an ester linkage.

0 Also encompassed by the invention are the Z pharmaceutically acceptable salts of the above-noted e- compounds.

ND Advantageous compounds of the invention are short-chain 00 10 (2 to 6 carbon atoms) fatty acid esters of uridine or C<1 Scytidine. Particularly advantageous compounds are triacetyluridine, triacetylcytidine or salts of orotic acid.

Inhibitors of uridine phosphorvlase As an alternative or adjunct to the above-noted pyrimidine nucleotide precursors, the following compounds are useful in the invention. These agents elevate tissue uridine nucleotide levels by inhibiting catabolism of endogenous or exogenous uridine. Co-administration of uridine phosphorylase inhibitors with pyrimidine nucleotide precursors reduces the amount of nucleotide precursor required to obtain therapeutic benefit.

Examples of inhibitors of uridine phosphorylase include but are not limited to 5-benzyl barbiturate or barbiturate derivatives including 5-benzyl barbiturate, benzyloxybenzyl barbiturate, 5-benzyloxybenzyl-l-[(1hydroxy-2-ethoxy)methyl] barbiturate, benzyloxybenzylacetyl-1-[(l-hydroxy-2-ethoxy)methyl] barbiturate, and 2,2'-anhydro-5-ethyluridine, and acyclouridine compounds, l^ particularly 5-benzyl substituted acyclouridine cohgeners D including but not limited to benzylacyclouridine, benzyloxybenzylacyclouridine, aminomethyl-benzylacyclouridine, 0 aminomethylbenzyloxybenzylacyclouridine, hydroxymethylbenzylacyclouridine, and hydroxymethyl-benzyloxybenzylacyclouridine. See also WO 89/09603 and WO 91/16315, hereby D incorporated by reference.

00 C 1 0 Detailed Description of the Invention (cN IThe subject invention relates to pyrimidine nucleotide precursors including acyl derivatives of cytidine, uridine, and orotate, and the use of these compounds and/or uridine phosphorylase inhibitors for treating or preventing pathological consequences of endotoxin and other inflammatory stimuli or mediators in animals, including humans.

The invention disclosed herein involves methods for enhancing the resistance of an animal to inflammatory stimuli and mediators. Examples presented below demonstrate both prophylaxis and treatment of toxicity due to endotoxin and 3o other inflammatory stimuli. The method of the invention can be used in conjunction with other methods for treating or preventing sepsis or systemic inflammation.

A. Definitions The term "pyrimidine nucleotide precursor" as used herein refers to a compound which is converted to a pyrimidine nucleotide following administration to an animal. This includes especially cytidine, uridine, or orotic acid, or prodrugs (including acyl derivatives) of these compounds.

The term "acyl derivative" as used herein means a O derivative of a pyrimidine nucleoside in which a substantially Cq nontoxic organic acyl substituent derived from a carboxylic Sacid is attached to one or more of the free hydroxyl groups of Z the ribose moiety of the oxypurine nucleoside with an ester 5 linkage and/or where such a substituent is attached to the amine substituent on the purine ring of cytidine, with an 00 amide linkage. Such acyl substituents are derived from carboxylic acids which include, but are not limited to, eCq compounds selected from the group consisting of a fatty acid, San amino acid, nicotinic acid, dicarboxylic acids, lactic C s15 acid, p-aminobenzoic acid and orotic acid. Advantageous acyl substituents are compounds which are normally present in the body, either as dietary constituents or as intermediary metabolites.

The term "pharmaceutically acceptable salts" as used herein means salts with pharmaceutically acceptable acid addition salts of the derivatives, which include, but are not limited to, sulfuric, hydrochloric, or phosphoric acids.

The term "coadministered" means that at least two of the compounds of the invention are administered during a time frame wherein the respective periods of pharmacological activity overlap.

The term "amino acids" as used herein includes, but is not limited to, glycine, the L forms of alanine, valine, leucine, isoleucine, phenylalanine, tyrosine, proline, hydroxyproline, serine, threonine, cysteine, cystine, methionine, tryptophan, aspartic acid, glutamic acid, 18 arginine, lysine, histidine, ornithine, hydroxylysine, Scarnitine, and other naturally occurring amino acids.

The term "fatty acids" as used herein means aliphatic Z carboxylic acids having 2-22 carbon atoms. Such fatty acids may be saturated, partially saturated or polyunsaturated.

The term "dicarboxylic acids" as used herein means fatty 00 acids with a second carboxylic acid substituent.

The term "therapeutically effective amount" as used herein refers to that amount which provides therapeutic C is effects for a given condition and administration regimen.

The term "sepsis" as used herein is a systemic inflammatory disorder in which endogenous cytokines and other 20 bioactive molecules, produced or released in response to an inflammatory stimulus such as bacterial endotoxin (a component of the cell wall of gram-negative bacteria), cause various symptoms including fever, neutropenia, blood coagulation disorders, hypotension, shock, and organ damage.

The term "inflammatory stimulus" as used herein means an exogenous agent which triggers an inflammatory response in an animal. Examples of inflammatory stimuli include bacteria, fungi, viruses, nonviable fragments or components of bacteria (such as endotoxin), fungi or viruses, or agents which trigger allergic or anaphylactic responses. In the case of autoimmune disorders, endogenous elements of a patient's tissues, e.g.

particular cellular proteins function as inflammatory stimuli.

The term "mediator" as used herein means endogenous or exogenous recombinant polypeptides) bioactive compounds,

C

N

I proteins, or polypeptides that typically mediate the O biological effects of endotoxin or other inflammatory stimuli such as fungal polysaccharides. Examples of such agents include but are not limited to tumor necrosis factor

(TNF),

interleukin-1 interleukin-6 plasminogen 00 activator inhibitor (PAI), leukotrienes, elements of the C N C complement cascade, nitric oxide, or platelet-activating CI factor.

B. Compounds of the Invention A primary feature of the present invention is the unexpected discovery that uridine and other pyrimidine nucleotide precursors do in fact protect otherwise normal animals animal models in which the organism has not received a clinically-irrelevant hepatotoxic sensitizing agent like galactosamine or TCDD) from toxicity due to bacterial endotoxin and other inflammatory stimuli which produce tissue damage through elicitation of endogenous inflammatory mediators.

Tissue uridine nucleotide levels can be increased by administration of several precursors. Uridine and cytidine are incorporated into cellular nucleotide pools by phosphorylation at the 5' position; cytidine and uridine nucleotides are interconvertible through enzymatic amination and de-amination reactions. Orotic acid is a key intermediate in de novo biosynthesis of pyrimidine nucleotides.

Incorporation of orotic acid into nucleotide pools requires cellular phosphoribosyl pyrophosphate (PRPP). Alternatively (or in addition to provision of exogenous nucleotide >precursors), availability of uridine to tissues is increased Z by administration of compounds which inhibit uridine 5 phosphorylase, the first enzyme in the pathway for degradation of uridine. The compounds of the invention useful in IN enhancing resistance to endotoxin or inflammatory mediators C 10 include uridine, cytidine, orotate, prodrug forms of these e- pyrimidine nucleotide precursors, particularly acyl O derivatives and phosphate esters, and inhibitors of the enzyme c 15 uridine phosphorylase. Compounds of the invention have the following structures: In all cases except where indicated, letters and letters with subscripts symbolizing variable substituents in the chemical structures of the compounds of the invention are applicable only to the structure immediately preceding the 2 description of the symbol.

An acyl derivative of uridine having the formula: 0 N

RIO

Rt0 OR3 wherein R, and R, are the same or different and each is Vt hydrogen or an acyl radical of a metabolite, provided that at O least one of said R substituents is not hydrogen, or a pharmaceutically acceptable salt thereof.

An acyl derivative of cytidine having the formula:

ID

00 NHR< 0

RIO

RzO ORa wherein R, and R, are the same or different and each is hydrogen or an acyl radical of a metabolite, provided that at least one of said R substituents is not hydrogen, or a pharmaceutically acceptable salt thereof.

The compounds of the invention useful in enhancing resistance to endotoxin include: An acyl derivative of uridine having the formula: Z

R

1 0 0 N R2O OR-

IND

oO 00 Swherein and R, are the same, or different, and each is Shydrogen or an acyl radical of S1 a. an unbranched fatty acid with 5 to 22 carbon atoms, b. an amino acid selected from the group consisting of glycine, the L forms of alanine, valine, leucine, isoleucine, tyrosine, proline, hydroxyproline serine, threonine, cystine, cysteine, aspartic acid, glutamic acid, arginine, lysine, histidine, carnitine and ornithine, c. a dicarboxylic acid having 3-22 carbon atoms, d. a carboxylic acid selected from one or more of the group consisting of glycolic acid, pyruvic acid, lactic acid, enolpyruvic acid, lipoic acid, pantothenic acid, acetoacetic acid, p-aminobenzoic acid, betahydroxybutyric acid, orotic acid, and creatine.

An acyl derivatives of cytidine having the formula: 23

NHR

0 N 00 2© o z 8 1 0 RO. OR, O Swherein and R, are the same, or different, and each is hydrogen or an acyl radical of 15 a. an unbranched fatty acid with 5 to 22 carbon atoms, b. an amino acid selected from the group consisting of glycine, the L forms of phenylalanine, alanine, valine, 0 leucine, isoleucine, tyrosine, proline, hydroxyproline, serine, threonine, cystine, cysteine, aspartic acid, glutamic acid, arginine, lysine, histidine carnitine and ornithine, c. a dicarboxylic acid having 3-22 carbon atoms, d. a carboxylic acid selected from one or more of the group consisting of glycolic acid, pyruvic acid, lactic acid, enolpyruvic acid, lipoic acid, pantothenic acid, acetoacetic acid, p-aminobenzoic acid, betahydroxybutyric acid, orotic acid, and creatine.

An acyl derivative of uridine having the formula:

HN

0

N

RIO,

OR,

wherein at least one of

R

2 or R, is a hydrocarbyloxycarbonyl moiety containing 2-26 carbon atoms and the remaining R substituents are independently a hYdrocarbyloxycarbonyl or hydrocarbylcarbonyl moiety or H or phosphate.

An acyl derivative of cytidine having the formula: 8 2 0 wherein at least one of

R

2 1 FR, o r R 4 is a hydrocarbyloxycarbonyl moiety containing 2-26 carbon atoms and the remaining R substituents are independently a hydrocarbyloxycarbonyl or hydrocarbylcarbonyl moiety or H or phosphate.

Orotic acid or salts thereof: co 0 o 0 HN 8 those in which the cationic component of the salt is sodium, 25

SOCOOHR

Pharmaceutically-acceptable salts of orotic acid include those in which the cationic component of the salt is sodium, 0 0 N

COOR,

H

wherein R. is a radical of an alcohol containing 1 to 20 carbon atoms joined to orotate via an ester linkage.

26 Also encompassed by the invention are the pharmaceutically acceptable salts of the above-noted compounds.

Advantageous compounds of the invention are short-chain (2 to 6 carbon atoms) fatty acid esters of uridine or cytidine. Particularly advantageous compounds are IND triacetyluridine or triacetylcytidine.

inhibitors of uridine ohosp)horvlase Examples of inhibitors of uridine phosphorylase. include but are not limited to 5-benzyl barbiturate or barbiturate derivatives including 5-benzy. barbiturate, benzyloxybenzyl barbiturate, 5-benzyloxybenzyll1 (1hydroxy-2-ethoxy)methyl] barbiturate, benzyloxybenzylacetyl-l.((l-hydroxy-2-ethoxy)methyl

I

barbiturate, and 2,2 '-anhydro-5-ethyluridine, 5-ethyl-2-deoxyuridine and acyclouridine compounds, particularly 5-benzyl substituted acyclouridine congeners including but not limited to benzylacyclouridine, bezlxbnyayluiie aminomethyl-benzylacyclouridine, aminomethylbenzyloxybenzylacyclouridine, hydroxymethyl-benzylacyclouridine, and hydroxymethyl...benzyloxybenzylacyclordine see also WO 89/09603 and WO 91/16315, hereby incorporated by reference..

Compositions ofthe Invention In one embodiment of the invention, novel pharmaceutical compositions comprise as an active agent one or more Spyrimidine nucleotide precursors selected from the group comprised of uridine, cytidine or orotic acid or its salts, and acyl derivatives of these pyrimidine nucleotide 0 precursors, together with a pharmaceutically acceptable carrier.

The compositions, depending on the intended use and route

I

N of administration, are manufactured in the form of a liquid, a C c suspension, a tablet, a capsule, a dragee, an injectable solution, or a suppository (see discussion of formulation Q below).

In another embodiment of the invention, the composition comprises at least one pyrimidine nucleotide precursor and an agent which inhibits the degradation of uridine, such as an inhibitor of the enzyme uridine phosphorylase. Examples of inhibitors of uridine phosphorylase include but are not limited to 5-benzyl barbiturate or 5-benzylidene barbiturate derivatives including 5-benzyl barbiturate, barbiturate, 5-benzyloxybenzyl-l-[(l-hydroxy-2-ethoxy)methyl] barbiturate, 5-benzyloxybenzylacetyl-l-[(l-hydroxy-2ethoxy)methyl] barbiturate, and acyclobarbiturate, 2,2'-anhydro-5-ethyluridine, and acyclouridine compounds, particularly 5-benzyl substituted acyclouridine congeners including but not limited to benzylacyclouridine, benzyloxybenzylacyclouridine, aminomethyl-benzylacyclouridine, aminomethylbenzyloxybenzyl- 4 acyclouridine, hydroxymethyl-benzylacyclouridine, and hydroxymethyl-benzyloxybenzylacyclouridine. See also US 5,077,280 and WO 91/16315, hereby incorporated by reference.

Furthermore, it is within the scope of the invention to utilize an inhibitor of uridine phosphorylase alone, without acoadministration of a pyrimidine nucleotide precursor, for the Z purpose of improving tissue resistance to endotoxin or inflammatory mediators.

In another embodiment, the compounds of the invention S0 include in addition to one or more compounds of the invention, c and at least one of the following compounds which are also V useful for treating endotoxin toxicity or.sepsis: Antibodies Sor other proteins which bind to endotoxin, TNF or IL-1; Polymyxin B conjugated to a polymeric support matrix (in order to reduce Polymyxin B toxicity while taking advantage of its capacity to bind and inactivate endotoxin); antagonists of 2 IL-1 or TNF receptors; antibiotics; inhibitors of the arachidonic acid cascade; arginine or ornithine; corticosteroids; glucose; ATP; purine nucleotide precursors including inosine, adenosine, or acyl derivatives thereof; cyclic AMP or acyl derivatives thereof.

In another embodiment of the invention, the composition comprises at least one compound of the invention and an antibacterial, antifungal, or antiviral compound.

Therapeutic Uses of the Compounds and Compositions of the Invention The compounds, compositions, and methods of the invention are useful to enhance resistance to endotoxin or other inflammatory stimuli or mediators in animals. The compounds include pyrimidine nucleotide precursors as well as compounds O which inhibit enzymatic degradation of uridine.

>The compounds and compositions of the invention are Z useful in treating mammals including humans; however, the invention is not intended to be so limited, it being within the contemplation of the invention to treat all animals that experience a beneficial effect from the administration of the C 10 active compounds of the invention.

q A primary feature of the invention is the discovery that O administration of uridine nucleotide precursors results in supra-normal resistance to toxic or lethal effects of endotoxin or other inflammatory stimuli or mediators in vivo.

The invention is furthermore embodied in the oral or systemic administration of a pharmaceutical compound or composition containing pyrimidine nucleotide precursors and/or agents which inhibit uridine catabolism, for the purpose of enhancing resistance to endotoxin, other inflammatory stimuli, or their mediators.

SIRS Sepsis and septic shock The compounds, compositions, and methods of the invention 3 are useful for reducing tissue damage due to systemic inflammatory response syndrome (SIRS), including sepsis, triggered by bacterial (both gram-positive and gram-negative), viral, fungal, or parasitic malaria) organisms. All of these types of infective organisms stimulate the formation or release of endogenous inflammatory mediators, resulting in tissue damage.

SThe compounds and compositions of the invention are D administered to patients with symptoms of sepsis, e.g. fever, neutropenia, hypotension, etc., or prophylactically to patients at risk for sepsis, e.g. surgical patients, patients with serious burns or wounds, or patients with urinary tract catheters.

\D

OO The compounds of the invention are optionally 00 administered in conjunction with other agents which are useful in treating sepsis, including but not limited to one or more of the following: Antibodies or other proteins which bind to 15 endotoxin, TNF or IL-1; Polymyxin B conjugated to a polymeric support matrix (in order to reduce Polymyxin B toxicity while taking advantage of its capacity to bind and inactivate endotoxin); antagonists of IL-1 or TNF receptors; antibiotics; inhibitors of the arachidonic acid cascade; leukotriene antagonists; arginine or ornithine; corticosteroids; glucose; ATP; inosine; cyclic AMP or acyl derivatives thereof. The compounds of the invention are administered either before, after, or during exposure of the animal or patient to one or more of these other agents.

For treatment or prevention of tissue damage due to sepsis, doses of the compounds of the invention ranging from about 0.5 to about 40 grams per day, advantageously 3 to grams per day, are administered, depending on the therapeutic response and the condition of the patient. In patients with serious sepsis syndrome, the compounds of the invention are typically administered in liquid or suspension form via a nasogastric tube, especially if such a tube is already in 31 place for delivery of nutrient suspensions or other enteral nutrition products. Patients with less serious illness CI typically receive compounds of the invention in either liquid 0 form, or in capsules or tablets. Patients who do not tolerate z oral administration of the compounds and compositions of the invention patients on total parenteral nutrition due to gastrointestinal tract damage) receive compounds of the

\D

00 invention that are sufficiently water soluble, such as uridine CA itself, by intravenous infusion.

C(

Following an episode of shock, trauma or sepsis, patients 15 often enter into a persistent state of hypermetabolism which can lead to multiple organ failure, usually beginning with hepatic failure. The hypermetabolic phase is due to the influence of endotoxin and its mediators on metabolic regulation (Cerra et al., in Molecular and Cellular Mechanisms of Septic Shock, 265-277, Alan R. Liss, 1989).

Hypermetabolism-organ failure is one of the leading causes of mortality among surgical intensive care patients. As demonstrated in the Examples, the compounds, compositions and methods of the invention are effective in reducing tissue damage and improving survival in animals subjected to endotoxin or other inducers of sepsis and organ failure. The compounds, compositions, and methods of the invention are useful in the treatment of patients at risk for hypermetabolic organ failure.

A serious consequence of sepsis is a propensity toward coagulation disorders, especially disseminated intravascular coagulation (DIC). In DIC, both blood coagulation and Sfibrinolysis are activated, so that blood clotting factors are rapidly consumed and aggregates of thrombin form in the circulation. DIC can result in either (or both) hemorrhage or 0 thrombus formation. The liver is the primary site for synthesis of clotting factors and for clearing microaggregates of thrombin from the circulation. The protective and therapeutic effects of the compounds, compositions, and 00 methods of the invention attenuate sepsis-induced alterations 10 C in blood coagulation (see Example 11).

SReduction of toxicity of therapeutic cytokines CN| Many of the biological effects of endotoxin and other inflammatory stimuli are mediated by the release of endogenous bioactive molecules (mediators) from target cells, particularly macrophages and Kupffer cells (sessile macrophages in the liver). Evidence for this is that macrophages in the C3H/HEJ strain of mice are genetically non-responsive to endotoxin (in terms of releasing cytokines upon exposure to endotoxin), and endotoxin is relatively nontoxic in this strain. These mice are however sensitive to bioactive peptides normally released from macrophages, e.g.

tumor necrosis factor (TNF), and toxicity of LPS is restored by transplantation of normal macrophages. TNF is generally held to be a primary mediator of endotoxin toxicity, but interleukin-1 (IL-1) and other agents also participate in the expression of endotoxin toxicity and sepsis.

Compounds, compositions, and methods of the invention are thus useful in modifying biological effects of inflammatory cytokines, whether produced endogenously (especially from macrophages), or introduced into the body from exogenous O sources polypeptides produced by recombinant DNA and fermentation technology).

O

Z Various inflammatory cytokines and even endotoxin itself

S

5 have potential therapeutic applications. Tumor necrosis factor, as suggested by its name, can destroy tumors and 0D synergizes with interferon-alpha in inhibiting viral 00 infections. Thus, TNF, and even bacterial endotoxin itself r n (which elicits the release of endogenous TNF), have been n administered to patients for the treatment of cancer. Classes 15 of inflammatory cytokines with both therapeutic activity and toxicity which limits their clinical use include TNF, interleukins and interferons. The compounds, compositions and methods of the invention are useful in preventing or treating toxicity which occurs during therapeutic administration of such cytokines as well as inflammatory stimuli.

When endotoxin is administered to cancer patients by intravenous infusion, hepatic toxicity limits the dose of endotoxin which can be administered (Engelhardt R et al., Cancer. Res. 1991 51:2524-30). In non-hepatic cancers, protection of the liver from endotoxin permits administration of higher doses of endotoxin in order to maximize its antitumor efficacy. Endotoxin also has immunostimulant properties. The compounds of the invention are thus useful for improving the therapeutic index of endotoxin, endotoxin analogs or derivatives Lipid A, Lipid X, Monophosphoryl Lipid A, etc.) or their mediators. Hepatic toxicity is also dose-limiting during intentional administration of TNF to humans (Kimura et al.,Cancr Cherer Phamaco. 1987, 20:223-229). Inflammatory stimuli of yeast or fungal origin, N such as the polysaccharides glucan or lentinan are also used O therapeutically as immunomodulators for treatment of z infections or cancer (Seljelid, Scand. J. Imunol. 1989, S29:181-92; Bowers et al., J. SurQ. Res. 1989;47:183-8).

Double-stranded RNA, such polyinosine-polycytidine, also has IOO therapeutic activity as an inflammatory stimulus for treatment C of cancer or infections.

(c n The inflammatory peptide Interleukin-1 which mediates some actions of endctoxin, similarly has important therapeutic potential in restoring hematopoiesis after damage caused by cancer chemotherapy), but its use is limited by toxic side effects which may be attenuated by utilization of the compounds, compositions, and methods of the invention.

Interleukin-2 (IL-2) is used clinically for treatment of several varieties of cancer; it also has potential activity as an imnunomodulator in treatment of various infections and in modulating the response to vaccines. Hepatic toxicity in response to IL-2 is not uncommon in patients receiving therapeutic doses of IL-2 for cancer treatment (Viens et al., J. Immunother, 1992 11:218-24). In an experimental model of autoimmune hepatitis induced by administration of concanavalin A to mice, hepatic damage is reported to be related to elevated production of endogenous IL-2 (Tiegs et al., J. Cin.

invgt.r 1992 90:196-203); as demonstrated in Example compounds, compositions, and methods of the invention are effective in attenuating hepatic damage in this model. The V compounds, compositions, and methods of the invention are useful in reducing side effects when administered in conjunction with IL-2; furthermore, the compounds, 0 compositions, and methods of the inventions are useful in treating autoimmune hepatitis.

Interleukin 6, which has therapeutic potential in IN improving blood platelet production, induces hepatic TNF receptors, thus increasing tissue sensitivity to TNF. The compounds, compositions, and methods of the invention are thus Suseful for use in combination with IL-6 or similar agents 15 which affect tissue senstivity to, or production of, TNF (Van Bladel et al., Cvtokine, 1991 3:149-54).

The combination of a particular therapeutic cytokine and 2 a pyrimidine nucleotide precursor and/or a uridine phosphorylase inhibitor is used for treatment of the disorders for which the particular therapeutic cytokine is known to be effective. For example, interleukin 2 is used for treatment of renal cancer, colon cancer, melanoma, lymphoma, leukemia and other neoplastic conditions. TNF has antitumor efficacy against a variety of cancer types, but its use in therapy has heretofore been limited by its toxicity, (Kimura et al., Cancer. Chemother. Pharmacol. 1987; 20:223-9). Endotoxin has shown significant antitumor efficacy (Engelhardt R et al., Cancer. Res. 1991 51:2524-30).

For prevention or treatment of toxicity due to 4 administration of therapeutic cytokines, approximately 0.5 to grams of a pyrimidine nucleotide precursor is administered daily for one to several days, depending on the duration of 36 the cytokine treatment. The pyrimidine nucleotide precursors 0 are administered before, during, or after administration of C the therapeutic cytokine. The therapeutic cytokines are 0 administered in the particular doses and regimens already established for experimental and clinical treatment of various forms of cancer, except that increased doses of cytokines may be tolerated when the pyrimidine nucleotide precursors of the

ID

00 invention are administered, as would be determined in simple CI dose-escalation studies for each cytokine or inflammatory stimulus.

C 15 Inflammatory Hepatitis: Liver disorders involving endotoxin or mediators The liver is susceptible to damage by endotoxin or its mediators, particularly when liver function is impaired.

Liver damage from many originating causes choline deficiency, Reye's syndrome, or alcohol) which either increase 2 hepatic sensitivity to endotoxin or inhibit endotoxin clearance, is in part mediated by bacterial endotoxin (normally present in the portal circulation due to leakage of small amounts from the intestine into the bloodstream) or mediators elicited by endotoxin (Nolan, Gastroenterology, 1975 69:1346-1356; Nolan, HepatoloQ_ 1989 10:887-91). Hepatic toxicity is dose-limiting in patients receiving intentional injections of endotoxin for possible efficacy in treating cancer (Engelhardt et al., Cancer Research, 1991 51:2524-2530).

As is demonstrated in the Examples below, the compounds, compositions, and methods of the invention significantly reduce hepatic damage induced by endotoxin and other Sinflammatory stimuli and mediators. The compounds, C- compositions, and methods of the invention are useful in O treating, preventing, or attenuating liver damage in a large variety of conditions in which hepatoxicity due to endotoxin or othe inflammatory stimuli or mediators are implicated in their etiology (whether or not systemic sepsis syndrome is 00 present). Conditions in which damage to the liver by

C

N CI endotoxin or its mediators TNF) are implicated include but are not limited to the following disease states: A. Reye's syndrome Reye's syndrome is characterized by rapid hepatic failure and is most commonly found in children as a complication of influenza and other viral infections; aspirin may be a risk factor. The etiology of Reye's syndrome is believed to involve endotoxin or inflammatory mediators. Endotoxemia is found in most or all patients with Reye's syndrome; an animal model for Reye's syndrome involves treating rats with a combination of endotoxin and aspirin (Kilpatrick et al., Metabolis., 1989, 38:73-7).

B. Alcoholic liver damage Excessive consumption of ethanol, in addition to problems associated with impaired mental and physical control associated with ethanol intoxication, is a significant cause of liver injury in humans. Endotoxin and TNF contribute to hepatic problems associated with exposure to alcohol. (Nolan JP, Henatologv 1989 10:887-91; Arai M, Nakano S, Okuno F, et 1 al. Hepatoloqy 1989; 9:846-851; McClain CJ and Cohen DA, SHepatolo 1989; 9:349-351).

c- C. Fulminant hepatitis 0 Z Tumor necrosis factor is implicated in the etiology and progression of. fulminant hepatitis, which can rapidly lead to hepatic failure and death (Aderka et al., Med Hypotheses, 1988 00 27:193-6) c CN| c- D. Viral hepatitis c- SEndotoxin contributes to hepatocyte damage occurring c 15 during viral hepatitis. Viral hepatitis reduces the LD .of endotoxin in animal models, and exclusion of endogenous endotoxin from experimental animals (by colectomy or by using axenic rodents) reduces the hepatic damage caused by a viral challenge. (Gut et al., J. Infect. Disease., 1984, 149:621).

In some cases of hepatitis, immune or inflammatory responses to hepatic viral infection mediated by T lymphocytes or macrophages contributes to liver damage. In either situation, the compounds, compositions, and methods of the invention are useful for treating hepatic damage related to viral infection.

Example 14 demonstrates that the compounds and methods of the invention improve survival in an animal model of viral hepatitis.

Immunopathology contributes to liver injury in viral hepatitis in humans. Hepatitis B and C viruses do not necessarily directly injure cells. There is substantial evidence that immune responses to infected cells contributes significantly to liver injury. Activated cytotoxic T lymphocytes attack antigen-bearing infected cells, but also 0 release cytokines like interferon-gamma which then recruit and CI activate inflammatory leukocytes in the liver and enhance o hepatic sensitivity to macrophage activators like endotoxin Z (Ando et al., J. Exp Med. 178:1541-1554, 1993). In Examples

S

5 and 12, beneficial effects of compounds, compositions, and methods of the invention are presented in experimental models 00 mimicking the key features of T Cell-mediated hepatic Cql CA inflammatory injury with and without secondary exacerbation CI caused by endotoxin. These Examples support the utility of 0 the compounds, compositions, and methods of the invention in I 5viral hepatitis, as well as in autoimmune hepatitis and cellmediated liver graft rejection.

E. Parasitic infections Hepatic damage and morbidity which occurs during malaria infection is mediated in part by TNF (Clark et al., Am. J.

Pathol. 1987, 129:192-9).

F. Hepatic damage during total parenteral nutrition Hepatic complications are common in patients receiving total parental nutrition (TPN) and who have no underlying liver disease; exacerbation of pre-existing liver injury also occurs during TPN. Pappo et al. Sura. Res., 1991, 51:106-12) reported that endotoxin (LPS) derived from the overgrowth of intestinal gram-negative bacteria is responsible for TPN-associated hepatic steatosis, and that bowel decontamination and specific anti-LPS activity of polymyxin B will reduce fatty infiltration of the liver during TPN.

Polymyxin B, which binds to and inactivates LPS, is toxic in humans, but served to demonstrate that hepatopathy observed during TPN is in fact mediated in part by endotoxin or TNF.

0 Therefore, inclusion of effective amounts of compounds of the invention in TPN solutions is useful for reducing TPN-induced liver damage, as well as for treating underlying inflammatory liver injury. Compounds of the invention, especially uridine, 00 cytidine, orotic acid, or water soluble salts and esters Sthereof, are either included in a TPN formulation or are qq administered separately but concurrently with TPN infusion. A Stypical TPN formula contains the basic nutrients needed to fulfill nutritional requirements in a form that is acceptable for intravenous administration. Thus, macromolecular dietary constituents like proteins or starches are provided in partially or fully digested form, e.g. as amino acids or sugars. A typical TPN formulation contains not only amino acids and sugars, but also other required nutrients like vitamins, minerals, and fats. Preferred doses of compounds of the invention to be used in conjunction with, or as constituents of, TPN formulas are in the range of 1 to grams per day (usually in the range of 2 to 20 grams per day) either as a bolus injection or as a sustained infusion.

In the context of this embodiment of the invention, a patient need not be receiving all of his or her nutrient requirements by the parenteral route in order to obtain benefit from the compounds, compositions, and methods of the invention. However, this embodiment of the invention is particularly advantageous where patients are receiving 50% or more of their nutrient requirements by intravenous infusion.

SLead poisoning C- Lead poisoning can dramatically increase sensitivity to endotoxin. Lead-induced interference with hepatic metabolism

O

Z is implicated in the effect of lead on endotoxin toxicity 2 (Taki et al., Eur. Surg. Res., 1985, 17:140-9).

H. Partial hepatectomy 00 q 10 Following partial hepatectomy for removal of

C

Cq cancerous tissue), morbidity and mortality from hepatic 0 failure is not uncommon. Liver tissue undergoing regeneration Cl 15 after partial hepatectomy in animals is hypersensitive to the deleterious effects of endotoxin and mediators (Shirai et al., Acta Pathol. Jpn., 1987, 37:1127-1134).

I. Postanesthetic hepatitis Inhalation anesthetics such as halothane can induce hepatitis, particularly-if hepatic bloodflow is also impaired.

Endotoxin is implicated in the etiology of postanesthetic hepatitis (Lomanto et al., Anesth. Anala., 1972, 51:264-270); the compounds of the invention are thus useful for administration to patients (prophylactically, therapeutically, or both) undergoing inhalation anesthesia for preventing and treating hepatitis. Trauma itself may contribute to postanesthetic hepatitis. Trauma furthermore often induces translocation of bacteria and endotoxin from the gut into other tissues via the bloodstream. Surgery patients are among the groups most susceptible to endotoxin poisoning (due to infection). Therefore, treatment of surgical patients with pyrimidine nucleotide precursors (before, during, or after Ssurgery) significantly improves their resistance to'endotoxin poisoning.

J. Cholestatic hepatitis z Hepatic injury due to bile duct obstruction or intrahepatic cholestasis is in part due to enterally-derived IN endotoxin. (Shibayama Y, 1989, J. Pathol. 159:335-9).

00 St 10 K. Liver transplantation V In patients receiving liver transplants, the presence of 0 high levels of endotoxin or inflammatory mediators preoperatively and at the end of the anhepatic period is associated with graft failure and a high mortality. Patients with primary nonfunction of their transplants typically have severe endotoxemia. Endotoxemia is implicated as a cause rather than an effect of perioperative complications and graft loss (Yokoyama et al, 1989, Transplant Proc. 21:3833-41). In 2 a clinical situation, an animal, such as a human, receives a compound of the invention enterally or parenterally after a transplant, in doses ranging from about 1 to about 40 grams per day, though typically 2 to 20 grams, advantageously divided into one to about four doses or else administered as a continuous or intermittent enteral or parenteral infusion. A compound of the invention is also optionally incorporated into an enteral or parenteral nutrition formulation prior to administration. Patients often receive intravenous isotonic glucose for several days after liver transplantation as an alternative to more complete parenteral or enteral nutrition. A compound of the invention, especially uridine or 43 cytidine, is advantageously formulated in an aqueous solution of 1 to 10 glucose. In a preferred embodiment, 1 to grams per day, advantageously 2 to 20 grams, of a pyrimidine 0 nucleotide precursor are administered per day. A secondary benefit of pyrimidine nucleotide precursors in liver disease or in recovery from a transplant is improved peripheral glucose utilization.

00 The donor liver can also be perfused with a solution C4 containing a compound of the invention, advantageously Suridine, cytidine, orotic acid or salts or acyl derivatives S 15 thereof, prior to or during implantation in the recipient. A pyrimidine nucleotide precursor, especially uridine, is included in a liver perfusion solution (also containing appropriate ions and other metabolites like glucose) at concentrations ranging from 10 micromolar to 10 millimolar.

Endotoxins and inflammatory mediators are also involved in other hepatic disorders; the diversity of the specific examples discussed above serve to indicate that the compounds, compositions, and methods of the invention are useful in treating or preventing a broad variety of liver diseases.

-For treatment of inflammatory hepatitis, 0.5 to 40 grams (advantageously 3 to 30 grams) of a pyrimidine nucleotide precursor are administered daily, advantageously divided into one to about four doses. The duration of the treatment regimen depends on improvement of clinical symptoms; acute O inflammatory liver disorders will typically require a shorter course of treatment than chronic degenerative conditions.

0° Other disorders As is demonstrated in Examples 2, 4-6, and 9, the compounds of the invention protect tissues other than liver, OO e.g. muscle, as indicated by serum creatine phosphokinase

C

0 (CPK) levels in animals treated with endotoxin or the fungal g inflammatory agent zymosan. Serum CPK activity is elevated as Sa consequence of damage to skeletal or heart muscle.

CN| Cachexia, a syndrome of weight loss, tissue wasting and misutilization of nutrients is a common complication in patients with cancer. TNF and other inflammatory cytokines are implicated in the initiation and maintenance of cachectic states; "Cachectin" is a synonym for TNF. The compounds, compositions, and methods of the invention are useful for treating patients with cachexia.

Clearance of ethanol from the circulation is a process that is largely dependent on energy metabolism and redox balance in the liver, in addition to levels of the enzyme alcohol dehydrogenase. Example 13 demonstrates that compounds of the invention improve recovery from severe ethanol intoxication. The compounds and compositions of the invention are useful in reducing the severity of both the mental and physical impairment due to alcohol intoxication as well as longer-term health consequences of chronic alcohol ingestion such as liver injury. Compounds of the invention (e.g.

triacetyluridine, uridine or cytidine) are administered orally before, during, or after ingestion of ethanol in doses of Sto 40 grams per day, advantageously 1 to 20 grams.

SVeterinary applications 0 z In horses and other large animals, there is a common syndrome known as laminitis, which is one consequence of endotoxin from the gut entering into the systemic circulation 00 (often after the animal overeats carbohydrate-rich foods, CN changing the bacterial populations in the gut). The Scompounds, compositions, and methods of the invention, since they attenuate tissue damage due to endotoxin, are useful in C~l treating or preventing laminitis and other effects of endotoxin poisoning in animals.

Administration and Formulation of Compounds and Compositions of the Invention The compounds and compositions of the invention are administered orally, by parenteral injection, intravenously, or by other means, depending on the condition being treated and the status of the patient.

The compounds and compositions of the invention are administered chronically, intermittently, or acutely as needed. In the case of an event which involves endotoxin toxicity or systemic inflammatory syndrome, the compounds and compositions are administered prior to, during, or after such event.

The pharmacologically active compounds optionally are combined with suitable pharmaceutically acceptable carriers n comprising excipients and auxiliaries which facilitate processing of the active compounds. These are administered as tablets, dragees, capsules, and suppositories. The Z compositions are administered for example orally, rectally, vaginally, or released through the buccal pouch of the mouth, and may be applied in solution form by injection, orally or by ND topical administration. The compositions may contain from 00 10 about 0.1 to 99 percent, preferably from about 50 to percent of the active compound(s), together with the excipient(s).

For parenteral administration by injection or intravenous infusion, the active compounds are suspended or dissolved in aqueous medium such as sterile water or saline solution.

Injectable solutions or suspensions optionally contain a surfactant agent such as polyoxyethylenesorbitan esters, sorbitan esters, polyoxyethylene ethers, or solubilizing agents like propylene glycol or ethanol. The solution typically contains 1 to 25% of the active compounds. In one embodiment of the invention, the aqueous medium is a solution of 1 to 10% glucose in water or isotonic saline. In some circumstances concurrent intravenous adminisration of glucose and a compound of the invention, especially uridine, is advantageous. Uridine (and acyl derivatives of uridine) improve glucose peripheral glucose utilization, and insulin (which is generally released from the pancreas in response to glucose or other carbohydrates or some amino acids) enhances nucleoside uptake and utilization by cells.

l For use in conjunction with parenteral nutrition, 0 compounds of the invention are dissolved or suspended in parenteral nutrition products, either during manufacture of 0 z such products or shortly prior to their administration to C 5 patients. The concentration of pyridimine nucleotide precursor-is adjusted in the parenteral nutrition formulation 4\ so that 1 to 40 grams, generally 2 to 20 grams, are delivered 00 C i1 per day during infusion of the parenteral nutrition product.

C<1 SA typically parenteral nutrition formula contains and delivers V) nutritionally adequate portions of amino acids, carbohydrates, 0 fats, vitamins, and minerals in sterile compositions suitable CKI 15 for intreavenous adminstration.

Suitable excipients include fillers such as sugars, for example lactose, sucrose, mannitol or sorbitol, cellulose preparations and/or calcium phosphates, for example tricalcium phosphate or calcium hydrogen phosphate, as well as binders such as starch paste, using, for example, maize starch, wheat starch, rice starch or potato starch, gelatin, tragacanth, methyl cellu-lose, hydroxypropylmethyl cellulose, sodium carboxymethyl cellulose and/or polyvinyl pyrrolidone.

Auxiliaries include flow-regulating agents and lubricants, for example, silica, talc, stearic acid or salts thereof, such as magnesium stearate or calcium stearate and/or polyethylene glycol. Dragee cores are provided with suitable coatings which, if desired, are resistant to gastric juices.

For this purpose, concentrated sugar solutions are used, which optionally contain gum arabic, talc, polyvinyl pyrrolidone, polyethylene glycol and/or titanium dioxide, lacquer solutions and suitable organic solvents or solvent mixtures. In order to produce coatings resistant to gastric juices, solutions of O suitable cellulose preparations such as acetylcellulose phthalate or hydroxypropylmethylcellulose phthalate are used.

Dyestuffs or pigments are optionally added to the tablets or dragee coatings, for example, for identification or in order N to characterize different compound doses.

00 The pharmaceutical preparations of the present invention are manufactured in a manner which is itself known, for Sexample, by means of conventional mixing, granulating, dragee-making, dissolving, or lyophilizing processes. Thus, pharmaceutical preparations for oral use are obtained by combining the active compound(s) with solid excipients, optionally grinding the resulting mixture and processing the mixture of granules, after adding suitable auxiliaries, if desired or.necessary, to obtain tablets or dragee cores.

Other pharmaceutical preparations which are useful for oral delivery include push-fit capsules made of gelatin, as well as soft-sealed capsules made of gelatin and a plasticizer such as glycerol or sorbitol. The push-fit capsules contain the active compound(s) in the form of granules which optionally are mixed with fillers such as lactose, binders such as starches and/or lubricants such as talc or magnesium stearate, and, optionally stabilizers. In soft capsules, the active compounds are preferably dissolved or suspended in 'suitable liquids such as fatty oils, liquid paraffin, or polyethylene glycols. In addition, stabilizers optionally are added. Other formulations for oral administration include solutions, suspensions, or emulsions. In particular, a liquid Sform suitable for administration via an enteral catheter, e.g.

a nasogastric tube, is advantageous, particularly for 0 bedridden or unconscious patients.

Pharmaceutical preparations which are used rectally include, for example, suppositories which consist of a ND combination of active compounds with a suppository base.

00 c-i Suitable suppository bases are, for example, natural or synthetic triglycerides, paraffin hydrocarbons, polyethylene Sglycols or higher alkanols. In addition, gelatin rectal capsules which consist of a combination of the active compounds with a base are useful. Base materials include, for example, liquid triglycerides, polyethylene glycols, or paraffin hydrocarbons.

Suitable formulations for parenteral administration include aqueous solutions of the active compounds in water soluble form, for example, water soluble salts. In addition, suspensions of the active compounds as appropriate in oily injection suspensions are administered. Suitable lipophilic solvents or vehicles include fatty oils, for example, sesame oil, or synthetic fatty acid esters, for example, ethyl oleate or tri-glycerides. Aqueous injection suspensions optionally include substances which increase the viscosity of the suspension which include, for example, sodium carboxymethylcellulose, sorbitol and/or dextran. The suspension optionally contains stabilizers.

Snthesis of the Comnounds of the Invention Acylated derivatives of pyrimidine nucleosides are synthesized by reacting a pyrimidine nucleoside or congener with an activated carboxylic acid. An activated carboxylic acid is one that has been treated with appropriate reagents to render its carboxylate carbon more susceptible to nucleophilic 00 attack than is the case in the original carboxylic acid.

Examples of useful activated carboxylic acids for synthesis of Sthe compounds of the invention are acid chlorides, acid Sanhydrides, n-hydroxysuccinimide esters, or carboxylic acids activated with BOP-DC. Carboxylic acids may also be linked to pyrimidine nucleosides or congeners with coupling reagents like dicyclohexylcarbodiimide

(DCC).

During preparation of the acyl compounds of the invention, when the acid source of the desired acyl derivative has groups which interfere with the acylation reactions, e.g., hydroxyl or amino groups, these groups are blocked with protecting groups, t-butyldimethylsilyl ethers or t-BOC S groups, respectively, before preparation of the anhydride.

For example, lactic acid is converted to 2 -t-butyldimethylsiloxypropionic acid with t-butyldimethylchlorosilane, followed by hydrolysis of the resulting silyl ester with aqueous base. The anhydride is formed by reacting the protected acid with DCC. With amino acids, the N-t-BOC derivative is prepared, using standard techniques, which is then converted to the anhydride with DCC. With acids containing more than one carboxylate group succinic, fumaric, or adipic acid) the acid anhydride of the desired dicarboxylic acid is reacted with a pyrimidine nucleoside in pyridine or pyridine plus dimethylformamide or dimethylacetamide.

0 Ss Amino acids are coupled to the exocyclic amino groups of cytidine, and to hydroxyl groups on the aldose moiety of pyrimidine nucleosides or their congeners, by standard methods 00 using DCC in a suitable solvent, particularly a mixture of (i) Cq '10 1 methylene chloride and (ii) dimethylacetamide or dimethylformamide.

C 15 Carbyloxycarbonyl derivatives of non-methylated pyrimidine nucleosides are prepared by reacting the nucleoside with the appropriate carbylchloroformate in a solvent such as pyridine or pyridine plus dimethylformamide under anhydrous conditions. The solvent is removed under vacuum, and the residue is purified by column chromatography.

It will be obvious to the person skilled in the art that other methods of synthesis can be used to prepare the compounds of the invention.

The following examples are illustrative, but not limiting Sof the methods and compositions of the present invention.

0Other suitable modifications and adaptations of a variety of conditions and parameters normally encountered in clinical therapy which are obvious to those skilled in the art are within the spirit and scope of this invention.

NO

01

EXAMPLES

K IM e Triacetyluridine and uridine improve survival in 0mice treated with killed E. Coli Purpose: Sepsis syndrome can be initiated by gram-negative bacteria even if they are not alive, since the primary trigger is endotoxin, a component of the bacterial cell wall. The purpose of this study was to determine the effect of oral triacetyluridine and parenteral uridine on survival of mice treated with a lethal dose of killed E. Coli bacteria Methods: Eighteen female Balb/C mice (eight weeks old) were divided into groups of six animals each. All mice received 500 micrograms of an acetone powder of E. Coli (serotype 0111:B4) suspended by sonication in 0.2 ml of saline. Mice in one group received uridine (2000 mg/kg in 0.2 ml saline) by i.p. injection two hours prior to administration of the E.

Coli. Another group of mice received triacetyluridine (6000 mg/kg in a vehicle of 1:1 corn oil/water containing 2.5 Tween 80) by oral intubation. Survival was monitored for one week.

O A. n 6 E. Coli (Control) SB. n 6 E. Coli (Control) Urd i.p.

5 C. n 6 E. Coli (Control) TAU p.o.

\D Results: 00 to O SAnimals in the Control group appeared to be in shock and were hypothermic 18 hours after administration of the E. Coli Spowder. Animals in the treated groups were active and maintaining body temperature, although their coats were scruffy throughout the first 48 hours of the observation period. Animals surviving 48 hours recovered completely. All of the mice treated only. with E. Coli died within 48 hours.

All mice treated with either uridine or triacetyluridine survived administration of killed E. Coli.

aE~E e2- Dose-response study of uridine in protection of tissues from endotoxin damage Purpose: The purpose of this study was to determine the doseresponse characteristics for uridine in prevention of inflammatory tissue damage caused by endotoxin

(LPS).

Methods: Female Balb/C mice (eight weeks old).were divided into six groups of six animals each. One group of animals remained untreated to provide basal values for serum chemistry indices ln of tissue damage. Mice in the remaining five groups received 0 100 micrograms of Salmonella Typhimurium endotoxin by i.p.

injection in a volume 0.2 ml saline. Two hours prior to 0 endotoxin administration, the five groups of mice received uridine in doses of 0, 500, 1000, 2000 and 4000 mg/kg i.p. (in 0.2 ml saline) respectively. Eighteen hours after endotoxin 0 administration, blood samples were collected for determination 00 C0 10 of serum chemistry values of indicators of tissue damage.

Cc0 C- Results: O Uridine produced a dose-dependent protection of tissues 15 against damage from endotoxin administration. ALT, AST, and SDH are specific indicators of liver damage; CPK is an indicator of damage to muscle; LDH is released from both liver and muscle. The most effective uridine dose in mice in this experiment was 2000 mg/kg.

Table 1: Uridine attenuates endotoxin-induced tissue damage

ALT

SDH

49±2 Basal (No LPS) Control (LPS) Uridine 500 Uridine 1000 Uridine 2000 Uridine 4000 198±124 137±26 708±177 906±211 3768±482 4176±459 8406±850 11628±2398 1170±157 2568±678 3090±871 5988±1225 8832±1089 834±192 1338±401* 1206±314* 3101±860* 4431±1529* 404±95* 605±236* 620±174* 1990±642* 4531±2139* 125±45* 1120±970* 744±457* 3441±2378* 8680±6746* 135±75* Different from Control (LPS P .02 ALT Alanine Aminotransferase AST Aspartate Aminotransferase LDH Lactate Dehydrogenase CPK Creatine Phosphokinase SDH Sorbitol Dehydrogenase Example 3: Oral triacetyluridine improves survival of mice treated with a lethal dose of Salmonella Typhimurium endotoxin Purpose: Sepsis syndrome caused by gram-negative bacteria is mediated primarily through endotoxin, a lipopolysaccharide constituent of the bacterial wall. The purpose of this experiment was to determine the effect of an orally- 0 administered uridine prodrug (Triacetyluridine; TAU) on survival of mice treated with a lethal dose of purified SSalmonella Typhimurium endotoxin (LPS).

z SMethods: Twenty female Balb/C mice (eight weeks old) were divided 0 into two groups of ten animals each. All mice received 100 00 10 micrograms of Salmonella Typhimurium endotoxin by intraperitoneal injection in 0.2 ml of saline. One group of V mice received triacetyluridine (6000 mg/kg in a vehicle of 1:1 C 15 corn oil/water containing 2.5 Tween 80) by oral intubation.

Survival was monitored for one week.

Results: All ten of the animals which received endotoxin alone died within 48 hours. Nine of the ten mice that received oral TAU survived for the seven day observation period and appeared to have recovered completely.

Exa mp le 4 Oral triacetyluridine reduces tissue damage caused by endotoxin Purpose: Bacterial endotoxin causes damage to the liver and other organs which can be assessed and quantified by determining serum levels of enzymes and other markers of tissue integrity and function. The purpose of this study was to determine the dose-response characteristics of orally-administered triacetyl uridine (TAU) in attenuating tissue damage due to endotoxin.

tI Methods: (Female Balb/C mice (eight weeks old) were divided into O .groups of five animals each. One group of animals remained z untreated to provide basal values for serum chemistry indices of tissue damage. Mice in the other four groups received 100 micrograms of Salmonella Typhimurium endotoxin by i.p.

00 injection, in a volume 0.2 ml saline. Three groups of 10 endotoxin-treated mice also received TAU 2 hours before 1 endotoxin in doses of 2000, 4000, and 6000 mg/kg by oral intubation in a volume of 0.4 ml. The TAU was formulated as a suspension in 1 carboxymethylcellulose in water. The remaining group (Controls) received the carboxymethylcellulose vehicle by oral intubation.

Results: Oral TAU administration reduced the levels of serum chemistry indicators of tissue damage. The beneficial effect on prevention of endotoxin-induced organ damage was dose dependent.

b Table 2: TAU attenuates endotoxin-induced tissue damage AJULP

SDH

ZBasal (No LPS) 130±46 148±32 563±132 41±5 Control (LPS) 3679±703 4798±927 6998±1064 1128±174 NOTAU 2000 263 2±915 3151±1085 5419±1561 793±294 00 TAU 4000 1463±382* 1940±456* 3878±672* 345±106* TAUJ 6000 365±91* 403±6'1* 1221±181* 104±18* *=Different from Control (LPS i.p. vehicle P .02 ALT Alanine Amninotransferase AST Aspartate Aminotransferase LDH Lactate Dehydrogenase CPK =Creatine Phosphokinase SDH Sorbitol Dehydrogenase Z~ranPjes_!_ Uridine reduces tissue damage in mice treated with carrageenan as a potentiator of endotoxin. toxicity Carrageenan is a polysaccharide derived from seaweed which modifies the activity of macrophages, which are principal cellular mediators of systemic inflammatory response to endotoxin. Macrophages release inflammatory peptides and other compounds in response to endotoxin. Carrageenan pretreatment sensitizes macrophages so that much less endotoxin than normal is required to elicit a serious systemic inflammatory response. Furthermore, a somewhat different spectrum of inflammatory mediators is involved in the toxic effects of the combination of carrageenan plus endotoxin z compared to endotoxin alone (Franks et al., Infecti an IBi ty, 59: 2609-2614 (1991]). The purpose of this experiment was to determine the effect of uridine on tissue D damage induced by a combination of carrageenan and endotoxin. 00 Cq Methods: SFemale Balb/C mice (eight weeks old) were divided into C five groups of six animals each. One group of animals remained untreated to provide basal values for serum chemistry indices of tissue damage. Mice in the other four groups received 2 mg of lambda carrageenan in 0.2 ml saline by i.p.

injection; three of these groups also received, one hour later, 2 micrograms of Salmonella Typhimurium endotoxin, also by i.p. injection in a volume 0.2 ml saline. Two of the groups that received both carrageenan and endotoxin also received uridine (2000 mg/kg i.p. in 0.2 ml saline); one group was treated with uridine 30 minutes after administration of endotoxin, and the other received 3 uridine pretreatments, 24, 6, and 2 hours before endotoxin administration, at 2000 mg/kg/dose i.p. Eighteen hours after endotoxin administration, blood samples were collected for determination of serum chemistry values of indicators of tissue damage.

Results: The combination of carrageenan with a low dose of endotoxin (2 mg) resulted in significant tissue damage as evaluated by serum chemistry indices. Treatment %iith uridine either before or after administration of endotoxin resulted in significant attenuation of tissue damage due to the carrageenan-endotoxin combination. Data are show-n below.

Table 3: Uridine attenuates endotoxin-induced tissue damage in carrageenan-sensiti zed mice

ALT

LD-H CPK

SDH

33±1 Basal (No LPS) Control (LPS) 223±77 141±35 700±145 747±278 Uridine 1937±235 2072±149 817±202* 989±139* 770±141* 891±79* 7360±354 4385±454* 4416±283* 11612±1513 107±17 5485±1638* 80±12* 5033±565* 117±9 Uridine (postreatment) *Different from Control, ALT Alanine Aminotransferase AST Aspartate Aminotransf erase LDH Lactate Dehydrogenase CPX Creatine Phosphokinase SDH Sorbitol Dehydrogenase V Example 6: Uridine improves survival in Zymosan-treated mice N Purpose:

O

Z Zymosan is a yeast component, primarily polysaccharide, which induces systemic inflammation and activation of complement. In fungal infections in general (including but IO not limited to yeast infections), such polysaccharides 00 Co participate in the induction of a sepsis response. Zymosan Sadministration to rodents is considered to be a suitable model Sfor multiple organ failure syndrome (Goris et al. (1986) Arch.

SSurg. 121:897-901; Steinberg et al. (1989) Arch. Sura.

124:1390-1395). Mortality at minimum lethal doses of zymosan is due in part to gut damage leading to translocation of bacteria and bacterial toxins from the gut into the bloodstream (Deitch et al., (1992) J. Trauma 32:141-147).

Methods: Female Balb/C mice (eight weeks old) were divided into groups of five animals each: 1. Zymosan 15 mg 2. Zymosan 15 mg Uridine 3. Zymosan 20 mg 4. Zymosan 20 mg Uridine Basal Zymosan A was suspended in mineral oil at a concentration of 50 mg/ml and administered by intraperitoneal injection.

Uridine (2000 mg/kg) was administered by intraperitoneal If injection in a volume of 0.2 ml two hours before administration of Zymosan.

0 18 hours after administration of Zymosan, blood samples were collected from both groups of mice that received 20 mg Zymosan and from a basal (untreated) group for subsequent measurement of serum chemistry indices of tissue damage.

00 Results: c 0 A. Survival at 48 hours: Group Survival Zymosan 15 mg/kg Zymosan 15 mg/kg Uridine Zymosan 20 mg/kg Zymosan 20 mg/kg Uridine B. Survival at 14 days (complete recovery) Zymosan 15 mg/kg Zymosan 15 mg/kg Uridine Uridine significantly improved survival time and incidence of long-term survivors among mice treated with Zymosan.

C.Serum chemistry indices of tissue damage Table 4: Uridine attenuates Zymosan-induced tissue damage ALT AST LDH CPK SDH 0 Basal 50±22 93±41 899±198 532±731 52±25 z Zymosan 397±140 392±97 1974±392 2107±1172 81±15 Zymosan Uridine 120±126 273±131 1419±244 754±370 58±22 Cc ALT Alanine Aminotransferase AST Aspartate Aminotransferase LDH Lactate Dehydrogenase CPK Creatine Phosphokinase SDH Sorbitol Dehydrogenase Example 7: Comparison of effects of uridine versus arginine on survival of endotoxin-treated mice Purpose: The amino acid arginine is reported to have beneficial effects in sepsis syndrome (Leon et al. J. Parenteral and Enteral Nutrition, 1991, 15:503-508). The purpose of this study was to compare the efficacy of uridine with that of arginine, an agent which supports liver function in sepsis syndrome and which is clinical use for this purpose.

Methods: Female Balb/C mice weighing 25 grams were divided into five groups of five or six animals each. Mice in the Sremaining five groups received 125 micrograms of Salmonella Typhimurium endotoxin (LPS) by i.p. injection in a volume 0.2 ml saline. Two hours prior to endotoxin administration, the 0 five groups of mice received injections of: 5 1) Saline (Controls) 2) Uridine 2000 mg/kg 3) Arginine 25 mg/kg N 10 4) Arginine 250 mg/kg Cg 5) Arginine 1250 mg/kg In SAll drugs were administered i.p. in 0.2 ml saline. The numbers of surviving mice in each group were determined 16, and 24 hours.

Results: Only one of the Control animals was alive 16 hours after LPS; in contrast,.the majority of the animals treated with uridine or arginine were alive at this point. However, by 24 hours after administration of endotoxin, the only surviving animals were in the group treated with uridine. All three doses of arginine did improve survival time (but did not produce any long-term survivors), and the lowest dose mg/kg) was more effective than the highest dose (1250 mg/kg).

Uridine was clearly more effective than arginine in promoting survival of endotoxin-treated animals.

Table 5: Effect of uridine vs arginine on survival after LPS administration Groups Control Uridine Arg 25 Arg 250 Arg 1250 Time after LPS (hr) 16 20 42 1/6 0/6 0/6 5/5 5/5 5/5 3/5 4/5 2/5 4/6 1/6 0/6 Example 8: Orotic acid improves survival of mice treated with Salmonella Typhimurium endotoxin Purpose: Sepsis syndrome caused by gram-negative bacteria is mediated primarily through endotoxin, a lipopolysaccharide constituent of the bacterial wall. The purpose of this experiment was to determine the effect of orotate on survival of mice treated with a lethal dose of purified Salmonella Typhimurium endotoxin.

Methods: Twenty female Balb/C mice (eight weeks old) were divided into two groups of ten animals each. One group of mice received four treatments with lysine orotate (200 mg/kg/dose; 9 AM and 2 PM on each of two consecutive days). Lysine o orotate is a water-soluble salt of orotic acid; lysine alone CI does not improve survival of endotoxin-treated mice. Control O animals received 0.2 ml of sterile water on the same treatment Sschedule. All mice received 100 micrograms of Salmonella STyphimurium endotoxin (LPS) by intraperitoneal injection in 0.2 ml of saline immediately after the last dose of lysine

ID

00 orotate. Survival was monitored for one week.

cN c- C( Results: 0 All of the mice in the Control group died within 48 hours. Nine of the ten mice treated with Lysine Orotate survived the full 72 hour observation period and were still alive and appeared to recover completely one week after LPS administration.

Table 6: Orotate improves survival of endotoxin-treated mice Survival after endotoxin treatment Time (hr after LPS) 2A 2 32. 4 72 Control 6/10 4/10 3/10 2/10 0/10 0/10 LOR 10/10 10/10 10/10 10/10 9/10 9/10 Example 9: Orotic acid protects tissues against endotoxin O damage 0 Purpose: z 5 The purpose of this study was to demonstrate the protective effect of orotic acid in prevention of inflammatory ND tissue damage caused by endotoxin.

00

N

C o10 Methods: Female Balb/C mice (eight weeks old) were divided into C 1 three groups of six animals each. One group of animals remained untreated to provide basal values for serum chemistry indices of tissue damage. Mice in the remaining two groups received 100 micrograms of Salmonella Typhimurium endotoxin (LPS) by i.p. injection in a volume 0.2 ml saline. Two hours prior to endotoxin administration, mice in one group received lysine orotate in a dose corresponding to 100 mg/kg of free orotic acid. Eighteen hours after endotoxin administration, blood samples were collected for determination of serum chemistry content of indicators of tissue damage.

Results: Orotate protected tissues against damage from endotoxin administration.

I

Table 7: Orotate attenuates endotoxin-jnduced tissue damage CIALT AST LP.D CPK aDH ZBasal (No LPS) 132±14 165±21 681±552 1258±233 42±1 Control (LPs) 2827±413 2860±506 6833±1167 6820±365 680±142 Orotate LPS 252±99* 415±77* 1641±274* 1040±283* 89±7* *=Different from Control (LPS P .02 ALT Alanine Amlintransferase AST =Aspartate Aminotransferase LDH Lactate Dehydrogenase CPX Creatine Phosphokinase -SDH Sorbitol Dehydrogenase Examrule 10: Uridine and triacetyluridijne attenuate hepatic damage caused by Concanavalin

A

Purpose: Interleukin-2 (IL-2) is used clinically for treatment of several varieties of. cancer. Hepatic toxicity in response to IL-2, is not uncommon in patients receiving therapeutic doses of IL-2 for cancer treatment (Viens et al., J. mmunother.

1992 11:218-24). In an experimental model of *autoimmune hepatitis induced by administration of Concanavalin A (Con A) to mice, hepatic damage is reported to be related to elevated production of endogenous IL-2 (Tiegs et al., J. Clin. Invest.

l 1992 90:196-203). The purpose of this study was to 0 demonstrate the utility of the compounds and methods of the invention in attenuating hepatic damage initiated by z administration of Con A.

5 Methods: \0 Female Balb/C mice (eight weeks old) were divided into 00 00 four groups of five animals each. One group of animals C ql 10 0 remained untreated to provide basal values for serum chemistry indices of tissue damage. Mice in the remaining three groups Sreceived 10 mg/kg Concanavalin A by intravenous (tail vein) injection in a volume of 0.2 ml saline. Two hours prior to receiving Con A, one of these groups of mice received uridine (2000 mg/kg i.p. in 0.2 ml saline) and another group received triacetyluridine (6000 mg/kg orally, in 0.6 ml of a 1:1 corn oil/water emulsion containing 2.5 Tween 80); the remaining Con A-treated group (Control) received 0.2 ml saline i.p. two hours prior to Con A. Twenty hours after administration of Con A, blood samples were collected from all mice for determination of serum levels of various indices of tissue damage or metabolic dysfunction.

Results: Con A administration resulted in significant damage to the liver, as assessed by serum levels of the enzymes ALT, AST, and SDH. Con A did not signficantly elevate levels of creatine phosphokinase an enzyme found primarily in muscle; tissue damage due to Con A in this model is more specifically localized in the liver than is damage due to

I

endotoxin. Uridine and TAU both reduced the liver damage produced by Con A administration, as shown in Table 8 below.

Table 8: Uridine and Triacetyluridine attenuate caused by Concanavalin A liver damage ALT LDH Basal (No Con A) 144±18 Con A 2652±847 Con A Uridine 289±115* 217±27 790±90 2765±1030 4335±1385 394±114* 973±202*

CPK

2392±370 2572±486 1996±317 1951±435

SDH

51±2 1114±318 163±68* 283±143* Con A TAU 575±286* 613±221 1380±270 Different from Control (LPS P .02 ALT Alanine Aminotransferase AST Aspartate Aminotransferase LDH Lactate Dehydrogenase CPK Creatine Phosphokinase SDH Sorbitol Dehydrogenase Liver damage in the Con A model used in this study is related to elevated IL-2 levels, and is mediated through T lymphocytes. Therefore, the compounds and methods of the invention are useful in reducing side effects due to therapeutic administration of IL-2; furthermore, the compounds and methods of the inventions are useful in treating autoimmune hepatitis.

71 Example 11: Uridine attenuates sepsis induced alterations in 0 blood coagulation Purpose:

O

Z Disseminated Intravascular Coagulation (DIC) is a serious consequence of sepsis, in which both blood coagulation and fibrinolysis are activated, so that blood clotting factors are

ND

00 rapidly consumed. DIC can result in hemorrhage or thrombus C( C- formation. The liver is the primary site for synthesis of C clotting factors and for clearing micro-aggregates of thrombin Sfrom the circulation. This purpose of this experiment was to determine the effect of pyrimidine nucleotide precursors on coagulation disorders induced by sepsis. Partial thromboplastin time was used as an index of the status of the blood coagulation system.

Methods: Thirty female Balb/C mice (eight weeks old) were divided into three groups of ten animals each. One group of mice remained untreated, and was used to determine basal values for partial thromboplastin time. Two groups of mice received received 30 mg/kg killed E. Coli (strain 0111:B4); Two hours before E. Coli administration, one group received uridine (2000 mg/kg) by intraperitoneal injection. 20 hours after E. Coli administration, plasma samples were collected from all thirty mice for determination of partial thromboplastin time (PTT). 0.27 ml of blood was collected via the retro-orbital plexus into a tube containing 0.03 ml of sodium citrate, pH 4. Plasma was separated by Scentrifugation, and 100 microliters of plasma was transferred to a clean 1.5 ml Eppendorf tube for determination of PTT with a commercial kit.

O

Z Results: Administration of killed E. Coli resulted in a prolongation of the normal partial thromboplastin time.

00 Uridine attenuated the sepsis-induced change in coagulation C 10 0 time, as shown in Table 9.

(c Table 9: Uridine attenuates sepsis-induced alterations in 5 is partial thromboplastin time Partial Thromboplastin Time Grou ETT (seconds) Basal (Normal) 32.3±1.3 E. Coli 69.8±5.4 E. Coli Uridine 51.2±2.1 different from control (E.Coli alone) value, P Exmpe 1: Combined liver injury due to T cells and endotoxin Several important forms of viral hepatitis as well as 4 autoimmune hepatitis are initiated by cytotoxic T cells which attac.c hepatocytes bearing appropriate viral or other antigens. Since endotoxin participates in liver damage initiated by a number of other agents like carbon tetrachloride, choline deficiency, ethanol, or cholestasis, studies were conducted to determine whether liver injury O caused by T cells induces hepatic hypersensitivity to z endotoxin. Following this experiment, the effect of TAU on combined liver injury due to both T lymphocytes and endotoxin was investigated.

IND

00 0C Example 12A: Concanavalin A potentiates endotoxin-induced C o10

S

tissue damage Groups of female Balb/C mice, age eight weeks, 1s received Concanavalin A (2.5 mg/kg endotoxin (Salmonella Typhimurium, 0.5 mg/kg), or a combination of Con A and endotoxin. The Con A was administered twenty four hours before endotoxin. Blood samples were taken 18 hours after injection of endotoxin (or its vehicle in the groups of mice that did not receive endotoxin). The "Basal" group of mice received vehicle only (saline) instead of Con A or endotoxin.

Table 1: Concanavalin A potentiates endotoxin-induced tissue damage ALT AST LDH CPK SDH Basal 87±15 110±9 656±41 413±87 39±2 Con A 2.5 mg/kg 117±19 170±16 915±46 419±132 42±4 LPS 0.5 mg/kg 119±23 256±22 881±10 426±82 41±3 Con A LPS 1130±494 2119±910 4370±1303 1525±450 471±267 i ALT Alanine Aminotransferase 0 AST Aspartate Aminotransferase CI LDH Lactate Dehydrogenase 0 CPK Creatine Phosphokinase Z SDH Sorbitol Dehydrogenase Endotoxin or Con A alone at the doses used in this experiment produced minimal damage to liver and muscle as 00 C t10 determined by serum enzyme levels (ALT, AST, LDH and SDH are (mf markers for liver damage; CPK is an indicator for muscle VB damage). However, in mice treated with the combination of Con SA and endotoxin, significantly greater damage was observed.

5 '15 The toxicity of Con A in this model is believed to be.

specifically related to T Lymphocyte-mediated liver damage (Tiegs et al., J. Clin. Invest. 90:196-203, 1992). Therefore, these results support the view that enterally-derived endotoxin participates in liver damage attributed to cytotoxic T lymphocytes in viral and autoimmune hepatitis), as has been demonstrated for liver damage initiated by other primary insults including carbon tetrachloride, choline deficiency, D-galactosamine, and viral infections.

Example 12B: TAU attenuates combined liver injury due to CTL's and endotoxin 3Experimental hepatitis initiated by intravenous administration of concanavalin A (Con A) is mediated by activation of cytoxic T lymphocytes. Liver injury in this model results in a marked increase in sensitivity to toxic effects of bacterial endotoxin. Sequential administration of Con A and endotoxin results in greater-than-additive hepatic fi V' injury (see Example 12 Hepatocyte injury in viral and O autoimmune hepatitis involves similar mechanisms, with damage initiated by T cells and exacerbated by enterally-derived 0 endotoxin and other inflammatory processes.

TAU protects the liver of experimental animals from damage initiated by either endotoxin or Con A. In this g experiment, TAU was tested for hepatoprotective effects in o 0 mice subjected to combined liver injury caused by sequential administration of both Con A and endotoxin.

Methods: Female Balb/C mice (eight weeks old) were divided into three groups of seven animals each. One group of animals 2 remained untreated to provide basal values for serum chemistry indices of tissue damage. Mice in the remaining two groups received 2 mg/kg Concancavalin A by intravenous (tail vein) injection in a volume of 0.2 ml saline, followed 24 hours later by Salmonella Typhimurium endotoxin (10 micrograms One of these groups of mice received TAU (6000 mg/kg orally, in 0.6 ml of 0.5% methylcellulose two hours before Con A and again 2 hours before endotoxin; the remaining Con A/endotoxin-treated group (Control) received vehicle (methylcellulose) alone. Eighteen hours after administration of endotoxin, blood samples were collected from all mice for determination of serum levels of various indices of tissue damage or metabolic dysfunction.

Results: Sequential administration of Con A and endotoxin resulted O in significant liver injury, as assessed by serum chemistry Z indices of liver damage. TAU, administered orally, markedly attenuates this combined liver injury.

Oral TAU attenuates liver damace caused by Concanavalin

A

LPs

SL

s LDH CPK SDH Basal 118±33 162±14 522±80 1521±235 56±3 Con A/LPS 2295±309 3408±389 5696±560 4684±1569 700±69 Con A/LPS 28567* 451±87* 1341±236* 2098±465* 122±19*

TAU

Different from Control (LPS p .02 ALT Alanine Aminotransferase AST Aspartate Aminotransferase LDH Lactate Dehydrogenase CPK Creatine Phosphokinase SDH Sorbitol Dehydrogenase

E

x a l e 1 3 Oral triacetyluridine improves recovery from ethanol intoxication Ethanol intoxication results in depression of activity in the central nervous system. Recovery is dependent upon clearance of ethanol from the system. Ethanol clearance from the circulation occurs primarily in the liver, regulated by both the enzyme alcohol dehydrogenase and the redox balance and metabolic state of the liver.

O

z In these experiments, ethanol-intoxicated mice were treated with triacetyluridine (TAU) in order to determine whether metabolic support through provision of uridine to the DO liver and other tissues affected recovery from ethanol 00 intoxication.

V Experiment 1: Fasted mice 1Methods Female Balb/C mice weighing an average of 22 grams were fasted for 24 hours. 9 mice received oral TAU 2000 mg/kg 2 and 8 received vehicle (0.75% Hydroxypropylmethylcellulose in water).

One hour later, all animals received 5.7 ml/kg ethanol ml of 25% aqueous solution One hour after EtOH, mice received an additional dose of TAU or vehicle. All mice were basically comatose at this time.

Behavior was monitored at hourly intervals, beginning 3 hours after ethanol administration. The scale for behavioral assessment is as follows: 78 Behavioral recovery after ethanol intoxication Deep coma: Unresponsive to stimuli. Slow respiration 0 Z Prostrate: Mice laying flat but not moving. Eyelid reflex to touching with a probe. Rapid respiration.

Righting reflex: When placed on its back, animal attempts 00 to turn over within 5 seconds. Category includes all "mobile" C\ 10 animals and some "prostrate".

Mobile: Animal is capable of walking.

0D TAU accelerates recovery from ethanol intoxication in fasted mice Control n 8 mice Time 3 hr 4 hr hr 6 hr 7 hr 8 hr Dead Deep coma Prostrate Righting Reflex Mobile 1 8 0 0 0 1 5 3 0 0 2 3 3 3 0 2 2 2 4 2 2 114 4 2 115 4 TAU n=9 mice Dead Deep coma Prostrate Righting Reflex Mobile 0 5 4 4 0 o 4 5 4 0 o 2 7 6 0 0 1 1 7 7 o 1 0 8 8 o 0 1 8 8 Time 3 hr 4 hr hr 6 hr 7 hr 8 hr Mice Female Balb/C, 22 grams, fasted 24 hours Ethanol dose 5.7 mIkg p.o. at time 0 hr (0.7 ml of 25 EtOR) TAU (2 glkg) or vehicle (control group) were administered one hour before and one hour after ethanol Experiment 2: Non-fasted mice Female Balb/C mice weighing an average of 22 grams were a al;lowed free access to food up to the time of the experiment.

mice received oral TAU 2000 mg/kg and 10 received vehicle (0.75% HPMC).

One hour later, all animals received 8 ml/kg ethanol (0.7 00 ml of 25% aqueous solution .one hour after E tO H, mice received an additional dose of STAU or vehicle.

Behavior was monitored at intervals of 2,3,4, and 6 hours after ethanol administration. The scale for behavioral assessment was the same as in the test on fasted mice above.

TAU accelerates recovery from ethanol intoxication in non-fasted mice Control n 10 mice Time 2 hr 3 hr 411hr 6 hr Time 2h I hr 43hr 6 hr Dead Deep coma Prostrate Righting Reflex Mobile 0 9 1 0 0 0 7 3 2 0 1 1 6 5 2 1 0 18 8 TAU n 10 mice Dead Deep coma Prostrate Righting Reflex Mobile 0 5 5 2 0 0 1 5 .5 3 0 0 3 8 7 0 0 0. 10 Mfice Female Balb/C, 22 grams, fed ad libijnm Ethanol dose 8 mllkg p.o. at time =0 hr (0.7 nil of 25 EtOH) TAU (2 g/kg) or vehicle (control group) were administered one hour before and one hour after ethanol TAU clearly improved behavioral recovery in mice g subjected to severe ethanol intoxication. Non-fasted mice were given a higher dose of ethanol than fasted animals (8 O ml/kg versus 5.7 ml/kg), but nevertheless recovered somewhat 0 faster. This observation highlights the importance of energy metabolism in recovery from ethanol intoxication.

TAU

accelerates recovery from ethanol intoxication in both fed and OO fasted animals.

C xaEle 14: Triacetyluridine reduces mortality in viral m hepatitis in mice q 5 Frog virus 3 (FV3) induces a rapidly fatal hepatitis in mice, which is mediated in part by secondary damage due to endogenous endotoxin (Gut et al., J. Infect. Disease., 1984, 149:621).

Triacetyluridine (TAU) was tested in this model to demonstrate that this agent and other compounds of the invention have useful therapeutic effects in viral hepatitis.

Methods: Lyophilized FV3 was reconstituted in phosphate-buffered saline to a density of 1 X 10 plaque-forming units (PFU) per ml.

Female Balb/C mice weighing 25 grams received FV3 virus at doses corresponding to the approximated LD,, by intraperitoneal or intravenous (tail vein) injection.

TAU

(3000 mg/kg) or vehicle (0.75 hydroxypropylmethylcellulose) was administered orally one hour before FV3 and on subsequent afternoons and mornings. Animals were observed for three days; the animals that did not survive this observation period all died approximately 24-30 hours after virus administration.

Intraperitoneal administration of FV3 Virus: FV3 4x10 7 PFU/mouse i.p.

Survival Control

TAU

6/10 10/10 Intravenous administration of FV3 Virus: FV3 2x10 7 PFU/mouse i.v.

Control

TAU

Survival 5/10 10/10 The foregoing is intended as illustrative of the present invention but not limiting. Numerous variations and modifications may be effected without departing from the true spirit and scope of the invention.

Claims (20)

1. A composition comprising a parenteral nutrition formula and (ii) 2 to 40 grams of a Spyrimidine nucleotide precursor per daily portion wherein said pyrimidine nucleotide precursor is uridine, cytidine, orotic acid, or an acyl derivative of uridine, cytidine, or orotic acid, or a O pharmaceutically acceptable salt thereof. 0 2. A method of providing nutrition to a mammal receiving nutrition intravenously comprising administering to said mammal a composition comprising 2 to 40 grams of a pyrimidine nucleotide ND precursor per daily portion. oo A composition comprising a pyrimidine nucleotide precursor when used in providing nutrition to a mammal receiving nutrition intravenously, wherein said composition comprises

2-40 grams of a pyrimidine nucleotide precursor per daily portion.

4. Use of a composition comprising a pyrimidine nucleotide precursor in the manufacture of Sa supplement for providing nutrition to a mammal receiving nutrition intravenously, wherein said composition comprises 2-40 grams of a pyrimidine nucleotide precursor per daily portion.

5. A composition comprising: a) glucose, and b) a pyrimidine nucleotide precursor, wherein said pyrimidine nucleotide precursor is uridine, cytidine, orotic acid, or an acyl derivative of uridine, cytidine, or orotic acid, or a pharmaceutically acceptable salt thereof, and wherein said pyrimidine nucleotide precursor is present in a concentration of between 1-25% by weight.

6. The composition of claim 5 wherein said composition is an aqueous solution containing 1 to 10% glucose.

7. The composition of claim 5 wherein said composition is an aqueous solution containing glucose.

8. The composition of any one of claims 5-7, wherein said pyrimidine nucleotide precursor is uridine or cytidine.

9. A method of treating a mammal during or after liver transplantation comprising administering a composition comprising glucose and a pyrimidine nucleotide precursor. A composition comprising glucose and a pyrimidine nucleotide precursor when used in treating a mammal during or after liver transplantation.

11. Use of a composition comprising glucose and a pyrimidine nucleotide precursor in the manufacture of a medicament for treating a mammal during or after liver transplantation.

12. A method of reducing inflammatory liver injury in an animal in need of such treatment comprising administering to said animal a therapeutically effective amount of an acyl derivative of uridine, cytidine or orotic acid, or a pharmaceutically acceptable salt thereof.

13. A method as in claim 12 wherein said acyl derivative of uridine is triacetyluridine. [I:\DayLib\LIBFF084 I 8b.doc:gcc

14. An acyl derivative of uridine, cytidine or orotic acid, or a pharmaceutically acceptable salt thereof, when used in reducing inflammatory liver injury in an animal in need of such treatment. The acyl derivative of uridine, cytidine or orotic acid, or a pharmaceutically acceptable salt thereof of claim 14, wherein said acyl derivative of uridine is triacetyluridine. O

16. Use of an acyl derivative of uridine, cytidine or orotic acid, or a pharmaceutically 0 acceptable salt thereof, in the manufacture of a medicament for reducing inflammatory liver injury in an animal in need of such treatment. I 17. The use of claim 16, wherein said acyl derivative of uridine is triacetyluridine. 0 18. A method for treating or preventing cachexia comprising administering to an animal a r o therapeutically effective amount of uridine, cytidine, orotic acid, or an acyl derivative of uridine, Scytidine, or orotic acid, or a pharmaceutically acceptable salt thereof. 0 19. A method as in claim 18 wherein said acyl derivative of uridine is triacetyluridine. (N 20. A method as in claim 18 or 19 further comprising administering an inhibitor of uridine phosphorylase. is 21. Uridine, cytidine, orotic acid, or an acyl derivative of uridine, cytidine, or orotic acid, or a pharmaceutically acceptable salt thereof when used in treating or preventing cachexia in an animal in need of said treatment or prevention.

22. The uridine, cytidine, orotic acid, or an acyl derivative of uridine, cytidine, or orotic acid, or a pharmaceutically acceptable salt thereof of claim 21, wherein said acyl derivative of uridine is triacetyluridine.

23. The uridine, cytidine, orotic acid, or an acyl derivative of uridine, cytidine, or orotic acid, or a pharmaceutically acceptable salt thereof of claim 21 or 22, wherein said treatment further comprises administering an inhibitor of uridine phosphorylase.

24. Use of uridine, cytidine, orotic acid, or an acyl derivative of uridine, cytidine, or orotic acid, or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for treating or preventing cachexia in an animal in need of said treatment or prevention. The use of claim 24, wherein said acyl derivative of uridine is triacetyluridine.

26. The use of claim 24 or 25, wherein said treatment further comprises administering an inhibitor of uridine phosphorylase.

27. A method for treating or preventing cachexia in an animal in need of said treatment or prevention, comprising administering to an animal a therapeutically effective amount of an inhibitor of uridine phosphorylase.

28. An inhibitor of uridine phosphorylase when used in treating or preventing cachexia in an animal in need of said treatment or prevention.

29. Use of an inhibitor of uridine phosphorylase in the manufacture of a medicament for treating or preventing cachexia in an animal in need of said treatment or prevention. [I:\DayLib\LIBFF]08418b.doc:gcc 86 A nutritional or pharmaceutical therapeutic or prophylactic formulation based on a 0 pyrimidine nucleotide precursor, wherein said pyrimidine nucleotide precursor is uridine, cytidine, c1 orotic acid, or an acyl derivative of uridine, cytidine, or orotic acid, or a pharmaceutically acceptable salt thereof, wherein said pyrimidine nucleotide precursor is provided in an amount between Z 5 2-40 grams per daily portion, substantially as hereinbefore described. Dated 10 November 2005 \o Pro-Neuron, Inc. oo 00 M I Patent Attorneys for the Applicant/Nominated Person SSPRUSON FERGUSON [I:\DayLib\LIBFF]0841 8b.doc:gcc