AU2003250811A1 - Methods and kit for genes shuffling using tagged primers - Google Patents

Methods and kit for genes shuffling using tagged primers

Info

Publication number
AU2003250811A1
AU2003250811A1 AU2003250811A AU2003250811A AU2003250811A1 AU 2003250811 A1 AU2003250811 A1 AU 2003250811A1 AU 2003250811 A AU2003250811 A AU 2003250811A AU 2003250811 A AU2003250811 A AU 2003250811A AU 2003250811 A1 AU2003250811 A1 AU 2003250811A1
Authority
AU
Australia
Prior art keywords
kit
methods
tagged primers
shuffling
genes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003250811A
Other versions
AU2003250811A8 (en
Inventor
Ana Claudia Cantisani Borges
Uffe Mortensen
Jens Nielsen
Michael Lynge NIELSEN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danmarks Tekniskie Universitet
Original Assignee
Danmarks Tekniskie Universitet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danmarks Tekniskie Universitet filed Critical Danmarks Tekniskie Universitet
Publication of AU2003250811A1 publication Critical patent/AU2003250811A1/en
Publication of AU2003250811A8 publication Critical patent/AU2003250811A8/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
AU2003250811A 2002-08-19 2003-08-15 Methods and kit for genes shuffling using tagged primers Abandoned AU2003250811A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US40413202P 2002-08-19 2002-08-19
US60/404,132 2002-08-19
PCT/DK2003/000545 WO2004016811A2 (en) 2002-08-19 2003-08-15 Methods and kit for genes shuffling using tagged primers

Publications (2)

Publication Number Publication Date
AU2003250811A1 true AU2003250811A1 (en) 2004-03-03
AU2003250811A8 AU2003250811A8 (en) 2004-03-03

Family

ID=31888328

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003250811A Abandoned AU2003250811A1 (en) 2002-08-19 2003-08-15 Methods and kit for genes shuffling using tagged primers

Country Status (2)

Country Link
AU (1) AU2003250811A1 (en)
WO (1) WO2004016811A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090162845A1 (en) * 2007-12-20 2009-06-25 Elazar Rabbani Affinity tag nucleic acid and protein compositions, and processes for using same
EP2397548A1 (en) 2010-06-18 2011-12-21 Hyglos Invest GmbH Methods of generating and screening for lytic chimeric polypeptides
WO2016144619A1 (en) * 2015-03-06 2016-09-15 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
US10221448B2 (en) 2015-03-06 2019-03-05 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
WO2016168351A1 (en) * 2015-04-15 2016-10-20 The Board Of Trustees Of The Leland Stanford Junior University Robust quantification of single molecules in next-generation sequencing using non-random combinatorial oligonucleotide barcodes
US9605305B2 (en) 2015-07-07 2017-03-28 Pillar Biosciences Inc. Method for reducing primer-dimer amplification

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9701425D0 (en) * 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
EP0973940B1 (en) * 1997-03-18 2008-07-02 Novozymes A/S An in vitro method for the construction of a dna library
CA2422749A1 (en) * 2000-09-21 2002-03-28 Merck & Co., Inc. Method for generating recombinant polynucleotides
ATE295882T1 (en) * 2000-12-12 2005-06-15 Alligator Bioscience Ab A METHOD FOR IN VITRO MOLECULAR EVOLUTION OF PROTEIN FUNCTION
US20030036641A1 (en) * 2001-01-31 2003-02-20 Padgett Hal S. Methods for homology-driven reassembly of nucleic acid sequences
WO2002079468A2 (en) * 2001-02-02 2002-10-10 Large Scale Biology Corporation A method of increasing complementarity in a heteroduplex polynucleotide

Also Published As

Publication number Publication date
WO2004016811A3 (en) 2004-03-25
AU2003250811A8 (en) 2004-03-03
WO2004016811A2 (en) 2004-02-26

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase