AU2002358463A1 - Oligonucleotide analogues comprising intercalator pseudonucleotides - Google Patents

Oligonucleotide analogues comprising intercalator pseudonucleotides

Info

Publication number
AU2002358463A1
AU2002358463A1 AU2002358463A AU2002358463A AU2002358463A1 AU 2002358463 A1 AU2002358463 A1 AU 2002358463A1 AU 2002358463 A AU2002358463 A AU 2002358463A AU 2002358463 A AU2002358463 A AU 2002358463A AU 2002358463 A1 AU2002358463 A1 AU 2002358463A1
Authority
AU
Australia
Prior art keywords
oligonucleotide analogues
intercalator pseudonucleotides
pseudonucleotides
intercalator
analogues
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002358463A
Other versions
AU2002358463A8 (en
Inventor
Ulf Bech Christensen
Erik Bjerregaard Pedersen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Human Genetic Signatures Pty Ltd
Original Assignee
UNEST AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UNEST AS filed Critical UNEST AS
Publication of AU2002358463A1 publication Critical patent/AU2002358463A1/en
Publication of AU2002358463A8 publication Critical patent/AU2002358463A8/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6832Enhancement of hybridisation reaction
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/13Decoys
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/30Chemical structure
    • C12N2310/31Chemical structure of the backbone
    • C12N2310/318Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
    • C12N2310/3181Peptide nucleic acid, PNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/30Chemical structure
    • C12N2310/32Chemical structure of the sugar
    • C12N2310/3212'-O-R Modification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/30Chemical structure
    • C12N2310/32Chemical structure of the sugar
    • C12N2310/323Chemical structure of the sugar modified ring structure
    • C12N2310/3231Chemical structure of the sugar modified ring structure having an additional ring, e.g. LNA, ENA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/30Chemical structure
    • C12N2310/35Nature of the modification
    • C12N2310/351Conjugate
    • C12N2310/3511Conjugate intercalating or cleaving agent
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/30Chemical structure
    • C12N2310/35Nature of the modification
    • C12N2310/351Conjugate
    • C12N2310/3517Marker; Tag
AU2002358463A 2001-12-18 2002-12-18 Oligonucleotide analogues comprising intercalator pseudonucleotides Abandoned AU2002358463A1 (en)

Applications Claiming Priority (19)

Application Number Priority Date Filing Date Title
DKPA200101898 2001-12-18
DKPA200101899 2001-12-18
DKPA200101900 2001-12-18
DKPA200101897 2001-12-18
DKPA200101897 2001-12-18
DKPA200101899 2001-12-18
DKPA200101898 2001-12-18
DKPA200101900 2001-12-18
US36554502P 2002-03-20 2002-03-20
US60/365,545 2002-03-20
DKPA200201577 2002-10-14
DKPA200201576 2002-10-14
DKPA200201576 2002-10-14
DKPA200201578 2002-10-14
DKPA200201575 2002-10-14
DKPA200201575 2002-10-14
DKPA200201578 2002-10-14
DKPA200201577 2002-10-14
PCT/DK2002/000875 WO2003052133A2 (en) 2001-12-18 2002-12-18 Oligonucleotide analogues comprising intercalator pseudonucleotides

Publications (2)

Publication Number Publication Date
AU2002358463A1 true AU2002358463A1 (en) 2003-06-30
AU2002358463A8 AU2002358463A8 (en) 2003-06-30

Family

ID=27576036

Family Applications (4)

Application Number Title Priority Date Filing Date
AU2002358464A Ceased AU2002358464B2 (en) 2001-12-18 2002-12-18 Pseudonucleotide comprising an intercalator
AU2002361946A Abandoned AU2002361946A1 (en) 2001-12-18 2002-12-18 Oligonucleotides comprising intercalator pseudonucleotide(s) for detection of nucleic acids and mutants hereof
AU2002358463A Abandoned AU2002358463A1 (en) 2001-12-18 2002-12-18 Oligonucleotide analogues comprising intercalator pseudonucleotides
AU2002358462A Abandoned AU2002358462A1 (en) 2001-12-18 2002-12-18 Linear and hairpin oligonucleotide analogues comprising intercalator pseudonucleotides

Family Applications Before (2)

Application Number Title Priority Date Filing Date
AU2002358464A Ceased AU2002358464B2 (en) 2001-12-18 2002-12-18 Pseudonucleotide comprising an intercalator
AU2002361946A Abandoned AU2002361946A1 (en) 2001-12-18 2002-12-18 Oligonucleotides comprising intercalator pseudonucleotide(s) for detection of nucleic acids and mutants hereof

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2002358462A Abandoned AU2002358462A1 (en) 2001-12-18 2002-12-18 Linear and hairpin oligonucleotide analogues comprising intercalator pseudonucleotides

Country Status (4)

Country Link
EP (1) EP1468007A2 (en)
CN (1) CN1653079B (en)
AU (4) AU2002358464B2 (en)
WO (4) WO2003052134A2 (en)

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AU2003900368A0 (en) * 2003-01-24 2003-02-13 Human Genetic Signatures Pty Ltd Assay for nucleic acid molecules
EP1594987B1 (en) * 2003-02-21 2009-05-27 GeneForm Technologies Limited Nucleic acid sequencing methods, kits and reagents
WO2004101816A1 (en) * 2003-05-15 2004-11-25 Human Genetic Signatures Pty Ltd Decoys for nucleic acid-binding proteins
US7799525B2 (en) 2003-06-17 2010-09-21 Human Genetic Signatures Pty Ltd. Methods for genome amplification
JP4714148B2 (en) 2003-09-04 2011-06-29 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド Nucleic acid detection assay
JP4397659B2 (en) * 2003-09-11 2010-01-13 独立行政法人科学技術振興機構 DNA detection method using molecular beacon using switching between monomer emission and excimer emission of fluorescent molecules
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
CN101056883B (en) * 2004-09-10 2012-10-10 人类遗传标记控股有限公司 Amplification blocker for intercalating nucleic acid (ina) containing intercalating pseudo nucleic acid (IPN)
US7671184B2 (en) 2004-09-24 2010-03-02 Universitact Bern Molecular beacons
KR101293713B1 (en) 2004-12-03 2013-08-12 휴먼 제네틱 시그너처스 피티와이 엘티디 Methods for simplifying microbial nucleic acids by chemical modification of cytosines
RU2487164C2 (en) * 2005-05-25 2013-07-10 ТИНА ХОЛДИНГ АпС Stable and selective formation of hoogsteen-type triplexes and duplexes using twisted intercalating nucleic acids (tina) and method for preparation of tina
CA2609218C (en) 2005-05-26 2016-10-11 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
ATE531820T1 (en) 2005-09-14 2011-11-15 Human Genetic Signatures Pty HEALTH CONDITION TEST
WO2007104318A2 (en) * 2006-03-16 2007-09-20 Pentabase Aps Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids
EP1845166A1 (en) * 2006-04-13 2007-10-17 BioSpring GmbH Nucleic acid probes with covalently bound intercalating fluorescent dyes for hybridization with target nucleic acids
WO2009027978A1 (en) 2007-08-30 2009-03-05 Hadasit Medical Research Services & Development Ltd. NUCLEIC ACID SEQUENCES COMPRISING NF-ϰB BINDING SITE WITHIN O(6)-METHYLGUANINE-DNA-METHYLTRANSFERASE (MGMT) PROMOTER REGION AND USES THEREOF FOR THE TREATMENT OF CANCER AND IMMUNE-RELATED DISORDERS
CN101918595A (en) 2007-11-27 2010-12-15 人类遗传标记控股有限公司 Enzymes for amplification and copying bisulphite modified nucleic acids
AU2008338530A1 (en) 2007-12-14 2009-06-25 Minitube Of America, Inc. Gender-specific separation of sperm cells and embryos
WO2011032034A2 (en) 2009-09-10 2011-03-17 University Of Idaho Nucleobase-functionalized conformationally restricted nucleotides and oligonucleotides for targeting nucleic acids
AU2011250350A1 (en) * 2010-05-07 2012-11-08 Quantibact A/S Method for generating a double stranded nucleic acid with a single stranded overhang
AR088140A1 (en) 2011-07-19 2014-05-14 Univ Idaho PROBE AND METHOD FOR DIRECTING NUCLEIC ACIDS
AU2011376698B2 (en) 2011-09-07 2014-07-17 Human Genetic Signatures Pty Ltd Molecular detection assay
WO2017045689A1 (en) 2015-09-14 2017-03-23 Pentabase Aps Methods and materials for detection of mutations
EP3484908A4 (en) 2016-07-15 2020-04-08 AM Chemicals Llc Non-nucleosidic solid supports and phosphoramidite building blocks for oligonucleotide synthesis
EP3803398A4 (en) * 2018-06-08 2022-03-23 Carnegie Mellon University Modified nucleobases with uniform h-bonding interactions, homo- and hetero-basepair bias, and mismatch discrimination
CN112601825B (en) * 2018-07-09 2023-11-28 青岛华大智造科技有限责任公司 Nucleic acid sequencing method
WO2020229510A1 (en) 2019-05-13 2020-11-19 Pentabase Aps Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids
CN111073884A (en) * 2020-02-14 2020-04-28 昆明理工大学 Method for improving accuracy of SNP locus detection with functional effect in SNP with distance of non-coding region less than 50bp
CN116209775A (en) 2020-06-29 2023-06-02 潘塔贝斯公司 Detection of methylation status

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Also Published As

Publication number Publication date
WO2003051901A2 (en) 2003-06-26
AU2002358464B2 (en) 2006-04-27
CN1653079A (en) 2005-08-10
AU2002358462A1 (en) 2003-06-30
WO2003052132A2 (en) 2003-06-26
AU2002358463A8 (en) 2003-06-30
WO2003051901A3 (en) 2003-11-27
AU2002361946A8 (en) 2003-06-30
EP1468007A2 (en) 2004-10-20
WO2003052132A3 (en) 2003-10-09
WO2003052133A2 (en) 2003-06-26
AU2002358462A8 (en) 2003-06-30
AU2002361946A1 (en) 2003-06-30
AU2002358464A1 (en) 2003-06-30
WO2003052133A3 (en) 2003-10-02
WO2003052134A3 (en) 2004-03-25
WO2003052134A2 (en) 2003-06-26
CN1653079B (en) 2010-06-16

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MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase