AU2002334203C1 - Kahalalide compounds for use in cancer therapy - Google Patents
Kahalalide compounds for use in cancer therapy Download PDFInfo
- Publication number
- AU2002334203C1 AU2002334203C1 AU2002334203A AU2002334203A AU2002334203C1 AU 2002334203 C1 AU2002334203 C1 AU 2002334203C1 AU 2002334203 A AU2002334203 A AU 2002334203A AU 2002334203 A AU2002334203 A AU 2002334203A AU 2002334203 C1 AU2002334203 C1 AU 2002334203C1
- Authority
- AU
- Australia
- Prior art keywords
- dose
- day
- mcg
- kahalalide
- use according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 150000001875 compounds Chemical class 0.000 title description 51
- 229930194861 kahalalide Natural products 0.000 title description 41
- 238000011275 oncology therapy Methods 0.000 title description 6
- 108010091711 kahalalide F Proteins 0.000 claims description 56
- RCGXNDQKCXNWLO-WLEIXIPESA-N (2r)-n-[(2s)-5-amino-1-[[(2r,3r)-1-[[(3s,6z,9s,12r,15r,18r,19s)-9-benzyl-15-[(2r)-butan-2-yl]-6-ethylidene-19-methyl-2,5,8,11,14,17-hexaoxo-3,12-di(propan-2-yl)-1-oxa-4,7,10,13,16-pentazacyclononadec-18-yl]amino]-3-methyl-1-oxopentan-2-yl]amino]-1-oxopent Chemical compound N([C@@H](CCCN)C(=O)N[C@H]([C@H](C)CC)C(=O)N[C@H]1C(N[C@@H](C(=O)N[C@@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)NC(/C(=O)N[C@H](C(=O)O[C@H]1C)C(C)C)=C\C)C(C)C)[C@H](C)CC)=O)C(=O)[C@H]1CCCN1C(=O)[C@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](NC(=O)CCCC(C)C)C(C)C)[C@@H](C)O)C(C)C)C(C)C RCGXNDQKCXNWLO-WLEIXIPESA-N 0.000 claims description 55
- 238000000034 method Methods 0.000 claims description 46
- 238000001802 infusion Methods 0.000 claims description 42
- 206010028980 Neoplasm Diseases 0.000 claims description 21
- 238000011282 treatment Methods 0.000 claims description 15
- 239000003814 drug Substances 0.000 claims description 11
- 230000000284 resting effect Effects 0.000 claims description 10
- 206010060862 Prostate cancer Diseases 0.000 claims description 9
- 208000000236 Prostatic Neoplasms Diseases 0.000 claims description 9
- -1 kahalalide F compound Chemical class 0.000 claims description 7
- 208000002154 non-small cell lung carcinoma Diseases 0.000 claims description 5
- 230000003442 weekly effect Effects 0.000 claims description 5
- 208000037844 advanced solid tumor Diseases 0.000 claims description 4
- 230000001394 metastastic effect Effects 0.000 claims description 4
- 206010061289 metastatic neoplasm Diseases 0.000 claims description 4
- 208000029729 tumor suppressor gene on chromosome 11 Diseases 0.000 claims description 4
- 208000037828 epithelial carcinoma Diseases 0.000 claims description 3
- 239000005556 hormone Substances 0.000 claims description 3
- 229940088597 hormone Drugs 0.000 claims description 3
- 206010027406 Mesothelioma Diseases 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 201000009030 Carcinoma Diseases 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 230000001988 toxicity Effects 0.000 description 31
- 231100000419 toxicity Toxicity 0.000 description 31
- 239000000203 mixture Substances 0.000 description 25
- 231100000371 dose-limiting toxicity Toxicity 0.000 description 24
- 231100000682 maximum tolerated dose Toxicity 0.000 description 19
- 230000002441 reversible effect Effects 0.000 description 12
- 150000001413 amino acids Chemical class 0.000 description 10
- 206010020751 Hypersensitivity Diseases 0.000 description 9
- 102000003929 Transaminases Human genes 0.000 description 9
- 108090000340 Transaminases Proteins 0.000 description 9
- 206010068237 Hypertransaminasaemia Diseases 0.000 description 8
- 201000011510 cancer Diseases 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- 239000002246 antineoplastic agent Substances 0.000 description 7
- 229940079593 drug Drugs 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 description 6
- 206010047700 Vomiting Diseases 0.000 description 6
- 231100000226 haematotoxicity Toxicity 0.000 description 6
- 231100000304 hepatotoxicity Toxicity 0.000 description 6
- 238000001990 intravenous administration Methods 0.000 description 6
- 239000008194 pharmaceutical composition Substances 0.000 description 6
- 241000479629 Elysia rufescens Species 0.000 description 5
- 239000002253 acid Substances 0.000 description 5
- 125000002252 acyl group Chemical group 0.000 description 5
- 125000000217 alkyl group Chemical group 0.000 description 5
- 208000026935 allergic disease Diseases 0.000 description 5
- 230000000259 anti-tumor effect Effects 0.000 description 5
- 125000003710 aryl alkyl group Chemical group 0.000 description 5
- 239000004067 bulking agent Substances 0.000 description 5
- 230000009610 hypersensitivity Effects 0.000 description 5
- 230000007056 liver toxicity Effects 0.000 description 5
- 231100000417 nephrotoxicity Toxicity 0.000 description 5
- 125000000962 organic group Chemical group 0.000 description 5
- 108090000765 processed proteins & peptides Proteins 0.000 description 5
- 230000004044 response Effects 0.000 description 5
- 241000196261 Bryopsis Species 0.000 description 4
- 108010002156 Depsipeptides Proteins 0.000 description 4
- 206010028813 Nausea Diseases 0.000 description 4
- 125000003118 aryl group Chemical group 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 230000001804 emulsifying effect Effects 0.000 description 4
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 4
- 230000006872 improvement Effects 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 230000008693 nausea Effects 0.000 description 4
- 102000004196 processed proteins & peptides Human genes 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 125000003396 thiol group Chemical group [H]S* 0.000 description 4
- 231100000331 toxic Toxicity 0.000 description 4
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 3
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 3
- 206010012735 Diarrhoea Diseases 0.000 description 3
- 206010020772 Hypertension Diseases 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 3
- 208000003251 Pruritus Diseases 0.000 description 3
- 125000003277 amino group Chemical group 0.000 description 3
- 239000003098 androgen Substances 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 231100000026 common toxicity Toxicity 0.000 description 3
- 125000002795 guanidino group Chemical group C(N)(=N)N* 0.000 description 3
- 125000005843 halogen group Chemical group 0.000 description 3
- 230000003278 mimic effect Effects 0.000 description 3
- 239000002736 nonionic surfactant Substances 0.000 description 3
- 150000007524 organic acids Chemical class 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000002588 toxic effect Effects 0.000 description 3
- 210000004881 tumor cell Anatomy 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 201000004384 Alopecia Diseases 0.000 description 2
- 206010006482 Bronchospasm Diseases 0.000 description 2
- KZSNJWFQEVHDMF-SCSAIBSYSA-N D-valine Chemical compound CC(C)[C@@H](N)C(O)=O KZSNJWFQEVHDMF-SCSAIBSYSA-N 0.000 description 2
- AOJJSUZBOXZQNB-TZSSRYMLSA-N Doxorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(=O)CO)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 AOJJSUZBOXZQNB-TZSSRYMLSA-N 0.000 description 2
- 206010015150 Erythema Diseases 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 2
- 108010054147 Hemoglobins Proteins 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000237852 Mollusca Species 0.000 description 2
- 208000002193 Pain Diseases 0.000 description 2
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 2
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 2
- UEIABLNYFAMSKG-UHFFFAOYSA-N Sacoglossan Natural products CC(=CCCC(=CC(CC(=CCC(OC(=O)C)C(=C/OC(=O)C)C=COC(=O)C)CO)OC(=O)C)C)C UEIABLNYFAMSKG-UHFFFAOYSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 231100000360 alopecia Toxicity 0.000 description 2
- 229940041181 antineoplastic drug Drugs 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000011953 bioanalysis Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000007885 bronchoconstriction Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 210000003169 central nervous system Anatomy 0.000 description 2
- 238000002512 chemotherapy Methods 0.000 description 2
- 229940109239 creatinine Drugs 0.000 description 2
- 125000004122 cyclic group Chemical group 0.000 description 2
- 230000001472 cytotoxic effect Effects 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 239000003937 drug carrier Substances 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 230000002489 hematologic effect Effects 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- YFGTYMAKKKYXPR-UHFFFAOYSA-N kahalalide K Natural products O=C1N2CC(O)CC2C(=O)NC(C)C(=O)NC(C(C)C)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)OC(CCCCCC(C)C)CC(=O)NC1CC1=CC=CC=C1 YFGTYMAKKKYXPR-UHFFFAOYSA-N 0.000 description 2
- 231100000225 lethality Toxicity 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 125000001570 methylene group Chemical group [H]C([H])([*:1])[*:2] 0.000 description 2
- 229930014626 natural product Natural products 0.000 description 2
- 208000004235 neutropenia Diseases 0.000 description 2
- 210000000440 neutrophil Anatomy 0.000 description 2
- 229940049964 oleate Drugs 0.000 description 2
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 210000002307 prostate Anatomy 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000004885 tandem mass spectrometry Methods 0.000 description 2
- 231100000440 toxicity profile Toxicity 0.000 description 2
- 230000008673 vomiting Effects 0.000 description 2
- SFLSHLFXELFNJZ-QMMMGPOBSA-N (-)-norepinephrine Chemical compound NC[C@H](O)C1=CC=C(O)C(O)=C1 SFLSHLFXELFNJZ-QMMMGPOBSA-N 0.000 description 1
- XQZOGOCTPKFYKC-VSZULPIASA-N (2r)-n-[(3s,6s,8s,12s,13r,16s,17r,20s,23s)-13-[(2s)-butan-2-yl]-12-hydroxy-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-2,5,7,10,15,19,22-heptaoxo-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]-4-methyl-2-(m Chemical compound C([C@H]1C(=O)O[C@H](C)[C@H](NC(=O)[C@@H](CC(C)C)NC)C(=O)N[C@@H]([C@H](CC(=O)O[C@H](C(=O)[C@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N2CCC[C@H]2C(=O)N1C)C(C)C)O)[C@@H](C)CC)C1=CC=C(OC)C=C1 XQZOGOCTPKFYKC-VSZULPIASA-N 0.000 description 1
- MWNLJNAPIYDPPC-FJVLBHDXSA-N (3r)-4-[[(2r)-1-[[(2s)-1-[[(2r)-1-[(2s,4r)-2-[[(2s)-1-[[(2r)-1-[[(1s)-1-carboxy-2-phenylethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-3-hydroxy-1-oxopropan-2-yl]carbamoyl]-4-hydroxypyrrolidin-1-yl]-3-methyl-1-oxobutan-2-yl]amino]-3-hydroxy-1-oxopropan-2-y Chemical compound C([C@@H](NC(=O)[C@@H](CC(O)=O)NC(=O)CC(O)CCCCCC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@H](C(C)C)C(=O)N1[C@@H](C[C@@H](O)C1)C(=O)N[C@@H](CO)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 MWNLJNAPIYDPPC-FJVLBHDXSA-N 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- XEMPUKIZUCIZEY-YSCHMLPRSA-N Barnidipine hydrochloride Chemical compound Cl.C1([C@@H]2C(=C(C)NC(C)=C2C(=O)OC)C(=O)O[C@@H]2CN(CC=3C=CC=CC=3)CC2)=CC=CC([N+]([O-])=O)=C1 XEMPUKIZUCIZEY-YSCHMLPRSA-N 0.000 description 1
- 108010006654 Bleomycin Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- ONIBWKKTOPOVIA-SCSAIBSYSA-N D-Proline Chemical compound OC(=O)[C@H]1CCCN1 ONIBWKKTOPOVIA-SCSAIBSYSA-N 0.000 description 1
- 101150029707 ERBB2 gene Proteins 0.000 description 1
- 241000939720 Elysia ornata Species 0.000 description 1
- 208000012671 Gastrointestinal haemorrhages Diseases 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 206010019837 Hepatocellular injury Diseases 0.000 description 1
- 206010019851 Hepatotoxicity Diseases 0.000 description 1
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 1
- 208000013038 Hypocalcemia Diseases 0.000 description 1
- 208000029663 Hypophosphatemia Diseases 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- JCYZMTMYPZHVBF-UHFFFAOYSA-N Melarsoprol Chemical compound NC1=NC(N)=NC(NC=2C=CC(=CC=2)[As]2SC(CO)CS2)=N1 JCYZMTMYPZHVBF-UHFFFAOYSA-N 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 206010029155 Nephropathy toxic Diseases 0.000 description 1
- 206010029260 Neuroblastoma Diseases 0.000 description 1
- 206010029350 Neurotoxicity Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 229930012538 Paclitaxel Natural products 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 229920002685 Polyoxyl 35CastorOil Polymers 0.000 description 1
- 229920001214 Polysorbate 60 Polymers 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 206010070308 Refractory cancer Diseases 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 206010039491 Sarcoma Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 206010044221 Toxic encephalopathy Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 description 1
- 231100000403 acute toxicity Toxicity 0.000 description 1
- 230000007059 acute toxicity Effects 0.000 description 1
- 125000002015 acyclic group Chemical group 0.000 description 1
- 229940009456 adriamycin Drugs 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 125000003342 alkenyl group Chemical group 0.000 description 1
- 230000006229 amino acid addition Effects 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229960001561 bleomycin Drugs 0.000 description 1
- OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 125000005518 carboxamido group Chemical group 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 230000003021 clonogenic effect Effects 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 238000002648 combination therapy Methods 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 229940127089 cytotoxic agent Drugs 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229930189582 didemnin Natural products 0.000 description 1
- 108010061297 didemnins Proteins 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
- 229930188854 dolastatin Natural products 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 239000000890 drug combination Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 231100000321 erythema Toxicity 0.000 description 1
- VJJPUSNTGOMMGY-MRVIYFEKSA-N etoposide Chemical compound COC1=C(O)C(OC)=CC([C@@H]2C3=CC=4OCOC=4C=C3[C@@H](O[C@H]3[C@@H]([C@@H](O)[C@@H]4O[C@H](C)OC[C@H]4O3)O)[C@@H]3[C@@H]2C(OC3)=O)=C1 VJJPUSNTGOMMGY-MRVIYFEKSA-N 0.000 description 1
- 229960005420 etoposide Drugs 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 208000011318 facial edema Diseases 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 125000005313 fatty acid group Chemical group 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 208000030304 gastrointestinal bleeding Diseases 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000001434 glomerular Effects 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 231100000844 hepatocellular carcinoma Toxicity 0.000 description 1
- 206010073071 hepatocellular carcinoma Diseases 0.000 description 1
- 231100000437 hepatocellular injury Toxicity 0.000 description 1
- 230000007686 hepatotoxicity Effects 0.000 description 1
- 208000018821 hormone-resistant prostate carcinoma Diseases 0.000 description 1
- 229960002591 hydroxyproline Drugs 0.000 description 1
- 230000000705 hypocalcaemia Effects 0.000 description 1
- 238000012606 in vitro cell culture Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000010253 intravenous injection Methods 0.000 description 1
- 108010091671 kahalalide B Proteins 0.000 description 1
- MWNLJNAPIYDPPC-UHFFFAOYSA-N kahalalide H Natural products C1C(O)CC(C(=O)NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC=CC=2)C(O)=O)N1C(=O)C(C(C)C)NC(=O)C(CO)NC(=O)C(NC(=O)C(CC(O)=O)NC(=O)CC(O)CCCCCC(C)C)CC1=CC=CC=C1 MWNLJNAPIYDPPC-UHFFFAOYSA-N 0.000 description 1
- BDIMJXXNMCZHLK-UHFFFAOYSA-N kahalalide J Natural products C1C(O)CC(C(=O)NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC=CC=2)C(=O)NC(CCCCN)C(O)=O)N1C(=O)C(C(C)C)NC(=O)C(CO)NC(=O)C(NC(=O)C(CC(O)=O)NC(=O)CC(O)CCCCCC(C)C)CC1=CC=CC=C1 BDIMJXXNMCZHLK-UHFFFAOYSA-N 0.000 description 1
- NEXGSZAIWOZENU-UHFFFAOYSA-N kahalalide O Natural products N1C(=O)C(NC(=O)CNC(=O)CCCC(C)C)C(C)OC(=O)C(C(C)C)NC(=O)C(C(C)CC)NC(=O)C(C(C)O)NC(=O)C(CC=2C3=CC=CC=C3NC=2)NC(=O)C1CC1=CC=C(O)C=C1 NEXGSZAIWOZENU-UHFFFAOYSA-N 0.000 description 1
- 108010090761 kahalalide O Proteins 0.000 description 1
- 229930190794 lamellarin Natural products 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 201000005296 lung carcinoma Diseases 0.000 description 1
- 210000003712 lysosome Anatomy 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 208000010658 metastatic prostate carcinoma Diseases 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 231100000483 muscle toxicity Toxicity 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- NEXGSZAIWOZENU-CDKWWZSLSA-N n-[2-[[(3s,6s,9s,12r,15r,18s,19r)-6-[(2s)-butan-2-yl]-9-[(1r)-1-hydroxyethyl]-15-[(4-hydroxyphenyl)methyl]-12-(1h-indol-3-ylmethyl)-19-methyl-2,5,8,11,14,17-hexaoxo-3-propan-2-yl-1-oxa-4,7,10,13,16-pentazacyclononadec-18-yl]amino]-2-oxoethyl]-5-methylhexa Chemical compound C([C@@H]1C(=O)N[C@H](CC=2C3=CC=CC=C3NC=2)C(=O)N[C@H](C(=O)N[C@H](C(N[C@H](C(=O)O[C@H](C)[C@H](NC(=O)CNC(=O)CCCC(C)C)C(=O)N1)C(C)C)=O)[C@@H](C)CC)[C@@H](C)O)C1=CC=C(O)C=C1 NEXGSZAIWOZENU-CDKWWZSLSA-N 0.000 description 1
- 230000007694 nephrotoxicity Effects 0.000 description 1
- 231100000228 neurotoxicity Toxicity 0.000 description 1
- 230000007135 neurotoxicity Effects 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- QUANRIQJNFHVEU-UHFFFAOYSA-N oxirane;propane-1,2,3-triol Chemical compound C1CO1.OCC(O)CO QUANRIQJNFHVEU-UHFFFAOYSA-N 0.000 description 1
- 229960001592 paclitaxel Drugs 0.000 description 1
- 201000002513 peritoneal mesothelioma Diseases 0.000 description 1
- 230000036470 plasma concentration Effects 0.000 description 1
- 239000008389 polyethoxylated castor oil Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229950008882 polysorbate Drugs 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 229940068968 polysorbate 80 Drugs 0.000 description 1
- 238000009101 premedication Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 208000016691 refractory malignant neoplasm Diseases 0.000 description 1
- OHRURASPPZQGQM-GCCNXGTGSA-N romidepsin Chemical compound O1C(=O)[C@H](C(C)C)NC(=O)C(=C/C)/NC(=O)[C@H]2CSSCC\C=C\[C@@H]1CC(=O)N[C@H](C(C)C)C(=O)N2 OHRURASPPZQGQM-GCCNXGTGSA-N 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 231100000873 signs of neurotoxicity Toxicity 0.000 description 1
- 210000002027 skeletal muscle Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 206010041823 squamous cell carcinoma Diseases 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- RCINICONZNJXQF-MZXODVADSA-N taxol Chemical compound O([C@@H]1[C@@]2(C[C@@H](C(C)=C(C2(C)C)[C@H](C([C@]2(C)[C@@H](O)C[C@H]3OC[C@]3([C@H]21)OC(C)=O)=O)OC(=O)C)OC(=O)[C@H](O)[C@@H](NC(=O)C=1C=CC=CC=1)C=1C=CC=CC=1)O)C(=O)C1=CC=CC=C1 RCINICONZNJXQF-MZXODVADSA-N 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 206010043554 thrombocytopenia Diseases 0.000 description 1
- 231100000048 toxicity data Toxicity 0.000 description 1
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/12—Carboxylic acids; Salts or anhydrides thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/12—Cyclic peptides, e.g. bacitracins; Polymyxins; Gramicidins S, C; Tyrocidins A, B or C
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Gastroenterology & Hepatology (AREA)
- Dermatology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Description
WO 03/033012 PCT/GB02/04735 KAHALALIDE COMPOUNDS FOR USE IN CANCER THERAPY FIELD OF THE INVENTION The present invention relates to the use of kahalalide compounds in the treatment of cancer.
BACKGROUND OF THE INVENTION Nature is the origin of many effective medicines in oncology, like paclitaxel, adriamycin, etoposide, bleomycin, etc. In recent years the sea has proven to be an invaluable source for compounds displaying original chemical structures and interesting biological activity. Among the cytotoxic compounds from marine origin we can mention the ecteinascidins, didemnins, dolastatins, spisulosines, lamellarins, some of them being developed as antitumoural agents in clinical trials.
The kahalalide compounds are peptides isolated from a Hawaiian herbivorous marine species of mollusc, Elysia rufescens. Kahalalides A-F are described in EP 610 078 and Hamman et al., J. Am. Chem. Soc., 1993, 115, 5825-5826.
Kahalalide A-G are described in Hamann, M. et J. Org. Chem, 1996, 61, 6594-6600: "Kahalalides: bioactive peptides from a marine mollusk Elysia rufescens and its algal diet Bryopsis sp.".
WO 03/033012 PCT/GB02/04735 2 Kahalalide H and J are described in Scheuer P.J. et al., J. Nat. Prod.
1997, 60, 562-567: "Two acyclic kahalalides from the sacoglossan mollusk Elysia rufescens".
Kahalalide O is described in Scheuer P.J. et al., J. Nat. Prod.
2000, 63(1) 152-4 A new depsipeptide from the sacoglossan mollusk Elysia ornata and the green alga Bryopsis species".
For kahalalide K, see Kan, Y. et J. Nat. Prod. 1999 62(8) 1169-72: "Kahalalide K: A new cyclic depsipeptide from the hawaiian green alga bryopsis species".
For related reports, see also Goetz et al., Tetrahedron, 1999, 7739-7746: "The absolute stereochemistry of Kahalalide Albericio, F. et al. Tetrahedron Letters, 2000, 41, 9765-9769: "Kahalalide B. Synthesis of a natural cyclodepsipeptide"; Becerro et al. J. Chem. Ecol. 2001, 27(11), 2287-99: "Chemical defenses of the sarcoglossan mollusk Elysia rufescens and its host Alga bryopsis sp." The synthesis and cytotoxic activities of natural and synthetic kahalalide compounds is described in WO 01 58934.
Of the kahalalide compounds, kahalalide F is the most promising because of its antitumoural activity. Kahalalide F now known to have the structure: WO 03/033012 PCT/GB02/04735 3 HN L- li..e
H
2 N-b r O D-Pro 0 N Thr N H N 0 D-Val H O0 NH 0 H -Val D-allle L-Va N H ZDhb NH HN H N -V-al HO -H L-Phe 0 L-Thr D-Val 5-MeHex Kahalalide F is a tridecapeptide with a ring shape side and a lateral side, containing a fatty acid group connected to the latter. Its activity against in vitro cell cultures of human lung carcinoma A-549 and human colon carcinoma HT-29 were reported in EP 610 078.
WO 02 36145 describes pharmaceutical compositions containing kahalalide F and new uses of this compound in cancer therapy.
See also Beijnen, J.H. et al., Drug Dev. Ind. Pharm. 2001, 27(8) 767- "Development of a lyophilized parenteral pharmaceutical formulation of the investigational polypeptide marine anticancer agent kahalalide F"; Beijnen, J.H. et al., Br. J. Clin. Pharmacol. 2002, 53(5), 543: "Bioanalysis of the novel peptide anticancer drug kahalalide F in human plasma by h.p.l.c.
under basic conditions coupled with positive turbo-ionspray tandem mass spectrometry"; Beijnen, J.H. et PDA J. Pharm. Sci. Technol. 2001, 55(4) 223-9 "In vitro hemolysis and buffer capacity studies with the novel marine anticancer agent Kahalalide F and its reconstitution vehicle cremophor EL/ethanol"; Sparidans R.W. et Anticancer Drugs 2001, 12(7) 575-82: "Chemical and enzymatic stability of a cyclic depsipeptide, the novel, marine-derived, anti-cancer agent kahalalide F".
WO 03/033012 PCT/GB02/04735 4 In preclinical studies, kahalalide F has shown significant activity against solid tumour cell lines, and selectivity for, but not restricted to, prostate tumour cells, neuroblastomas, some primary sarcoma lines and tumour cells that overexpress the Her2/neu oncogene. In vitro exposure studies demonstrated that kahalalide F is not schedule dependent. Its mechanism of action is not yet elucidated, in vitro studies have shown activity of kahalalide F to cause cell swelling and ultimately death, see for example Garcia-Rocha M, Bonay P, Avila Cancer Lett. 1996 99(1) 43-50: "The antitumoural compound Kahalalide F acts on cell lysosomes".
Preclinical in vivo studies determined that the maximum tolerated dose (MTD) of KF in female mice following a single bolus iv injection was to be 280 pg/kg. Whereas single doses just above the MTDiv were extremely toxic, with animals exhibiting signs of neurotoxicity followed by death, 280 pg/kg KF could be administered repeatedly, according to a once daily times five schedule, without any apparent evidence of acute toxicity. See Supko, F. et al., Proceedings of the 1999 AACR NCI EORTC International Conference, abstract 315: "Preclinical pharmacology studies with the marine natural product Kahalalide F".
During preclinical studies kahalalide F exhibited low cardiac and skeletal muscle toxicities and also low myeolotoxicity. In mice, the main toxicities found were tubular nephrotoxicity and CNS (central nervous system) neurotoxicity, and hints of hepatotoxicity. Therefore the expected toxicities were renal and neurologic with a marked threshold. As mentioned before, whereas MTD had no lethality, doses slightly over MTD showed high lethality.
It is an object of the present invention to provide new, improved forms SUMMARY OF THE INVENTION We have developed a method to treat human patients with kahalalide compounds, in particular kahalalide F, avoiding toxicity and leading to clinical improvement.
We have found that contrary to what was expected, the dose limiting toxicity in the treatment with a kahalalide compound is liver toxicity with grade 4 transaminase elevation. This toxicity is asymptomatic, manageable and reversible if the proper dosages and schedules are selected. Transaminases peak 4-5 hours after kahalalide infusion.
There is provided a procedure for establishing the amount of a kahalalide compound to be recommended for dosing to patients, which procedure comprises administering the compound in a series of escalating doses to a cohort of humans, monitoring for transaminase elevation as the doselimiting toxicity, determining a maximum tolerated dose, and establishing a recommended dose.
Such a procedure can be used in clinical trials. The maximum tolerated dose is suitably set as one in which a proportion of the cohort encounter doselimiting toxicity. For example, the proportion is typically 2 out of 6. The recommended dose can then be established in accordance with familiar principles. Usually the recommended dose is the dose below the maximum tolerated dose, but sometimes rules are applied regarding the proportion of the cohort which encounter dose-limiting toxicity at the proposed recommended dose.
N\Melbourne\Casea\Patent\52000-52999P52651 .AU\Specis\P52651 AU~doc 29/06/07 13/12 2007 15:13 FAX 61-3 92438333 GRIFFITH HACK IPAUSTRALIA Q005 o -6- 0 C The procedure for clinical trials enables a method of preparing a pharmaceutical composition containing a kahalalide compound, the method 0 comprising carrying out the procedure to establish the recommended dose, and Sformulating the kahalalide compound with a pharmaceutically acceptable carrier to give a dosage form which contains the recommended dose of the en kahalalide compound.
0 ci en The invention provides for the use of kahalalide compounds in the ci preparation of a medicament for the procedures and methods of this invention, particularly as outlined below in the following method aspects of the invention.
In one aspect, the present invention provides a method for treating a human patient afflicted with cancer, comprising administering to said patient a kahalalide compound at a dose below 1200 mcg/m2/day, preferably below 930 is mcg/m2/day and more preferably below 800 mcg/m2/day. Suitably the dose is at least 320 mcg/m2/day. Preferably the dose is in the range of 400-900 mcg/m2/day, preferably 500-800 mcg/m2/day, more preferably 600-750 mcg/m2/day. Especially preferred are doses of about 650-700 mcg/m2/day.
We have found that the selected schedule is impo:rtant to allow for a reversion of the liver toxicity. If a daily 1 hour intravenous infusion is used during 5 days, the dose limiting toxicity is reached at 930 mcg/m2/day.
Thereafter a rest period of 2 weeks is needed to reverse the toxicity effects.
Therefore in one aspect the invention provides a method for treating a human patient afflicted with cancer, comprising administering to said patient a kahalalide compound daily during 5 days at a dose below 930 mcg/m2/day, N:\MelboulnCcSTueMM20'-52999\?52651 A SpciaPI265 LAU Sp ric tion 2007-124.doC 13/12107 COMS ID No: ARCS-172267 Received by IP Australia: Time 16:12 Date 2007-12-13 7 followed by a resting period of from 1 to 4 weeks in which the kahalalide compound is not administered. The dose is preferably 650-750 mcg/m2/day, more preferably about 700 mcg/m2/day. The infusion time is preferably between 1 and 24 hours, more preferably between 1 and 3 hours. Especially preferred is an infusion time of about 1 hour. The resting period N:\Melbourne\Cases\Patent\52O00-52999\P5265 .AU\Specis\P52651.AU.doc 29/06/07 WO 03/033012 PCT/GB02/04735 8 is preferably 2-3 weeks, more preferably about 2 weeks.
Surprisingly for this kind of cancer therapy, we also found that a weekly schedule is possible without resting period if the dosage is about 650 mcg/m2/day. Transaminases elevation is reversible by day 8th at about 650 but not at 800. In this case the liver toxicity was found to be reversible within one week and no resting period is needed, with the obvious advantages this supposes.
Therefore, in another aspect the present invention provides a method for treating a human patient afflicted with cancer, comprising administering to said patient a kahalalide compound once weekly at a dose below 800 mcg/m2/day. The dose is preferably 600-700 mcg/m2/day, more preferable 650 mcg/m2/day. The infusion time is preferably between 1 and 24 hours, more preferably between 1 and 3 hours. Especially preferred is an infusion time of about 1 hour.
The above schedules and dosages allow for an effective cancer therapy in humans. We have found that kahalalide compounds, and in particular kahalalide F is effective in the treatment of advanced solid tumours (AST), including metastatic tumours. Tumours that are preferably treated are hormone independent prostate cancer, hepatocarcinoma, epithelial carcinomas, non small cell lung cancer and mesothelioma.
In a further aspect of the present invention, a medical kit for administering a kahalalide compound is provided, comprising printed instructions for administering the kahalalide compound according to the dosing schedules set forth above, and a supply of kahalalide compound in dosage units for at least one cycle, wherein each dosage unit contains the WO 03/033012 PCT/GB02/04735 9 appropriate amount of kahalalide compound for the treatments as defined above and a pharmaceutically acceptable carrier.
DETAILED DESCRIPTION The term "kahalalide compound" includes natural compounds, their mixtures and new compounds as defined in WO 01 58934 which is incorporated herein in its entirety by reference. Especially preferred is the compound kahalalide F.
Thus, the present invention employs a natural kahalalide such as kahalalide F or a mimic of a natural kahalalide. The mimic compounds may differ in one or more amino acids, and one or more components of the acyl side chain. Preferably they differ in one or more components of the acyl side chain. Examples of the kahalalide compound for use in this invention particularly include the compound identified as kahalalide F with a methylhexyl sidechain, compounds differing only in the sidechain such as the 4-methylhexyl analogue, and mixtures thereof.
Suitably the mimics have at least one of the following features to differentiate from a parent naturally occurring kahalalide: 1 to 7, especially 1 to 3, more especially 1 or 2, most especially 1, amino acid which is not the same as an amino acid the parent compound; 1 to 10, especially 1 to 6, more especially 1 to 3, most especially 1 or 2, additional methylene groups in the side chain acyl group of the parent compound; WO 03/033012 PCT/GB02/04735 1 to 10, especially 1 to 6, more especially 1 to 3, most especially 1 or 2, methylene groups omitted from the side chain acyl group of the parent compound; 1 to 6, especially 1 to 3, more especially 1 or 3, substituents added to or omitted from the side chain acyl group of the parent compound.
For cyclic kahalalides, the amino acid addition or omission can be in the cyclic ring or in the side chain.
Examples of mimic comopunds are compounds related to kahalalide F, and having the formula: Aaa-7 R N OH Aaa-1 H
H
S
R
2 N O Xz-NH H X2-N
N
0 Aaa-3 Aaa-4 Rs Formula II wherein Aaal, Aaa 2 Aaa 3 Aaa 4 Aaa 6 and Aaa 7 are independently a-amino acids of L or D configuration, if applies; wherein R 1
R
2 R3, R4, R5, R6, R7 are each independently H or an organic group selected from the group consisting of an alkyl group, an aryl group, an aralkyl group, and their substituted derivatives with an hydroxy group, a mercapto group, an amino group, a guanidino group, a halogen group; wherein X1 is independently 0, S, or N; wherein R2 is, if applies, independently H or an organic group selected WO 03/033012 PCT/GB02/04735 11 from the group consisting of an alkyl group and an aralkyl group; wherein is independently an amino acid of L or D configuration, if applies; wherein X2 is independently an organic group selected from the group consisting of an alkenyl, an alkyl group, an aryl group, an aralkyl group, and their substituted derivatives with an hydroxy group, a mercapto independently H or an organic group selected from the group consisting of an alkyl group, an aryl group, an aralkyl group, and their substituted derivatives with an hydroxy group, a mercapto group, an amino group, a guanidino group, a halogen group; wherein R8 is independently of the following formulae III, IV, or V: 10 o
RN
0 R9 'R Formula III o R 9 0
R
1 1
N
n Formula IV O 0 Ri, N R9 n Formula V wherein R9, RIO, and R11 are each independently H or an organic group selected from the group consisting of an alkyl group, an aryl group, an aralkyl group, and their substituted derivatives with an hydroxy group, a mercapto group, an amino group, a guanidino group, a carboxyl group, a carboxamido group, a halogen group; R9 and R10 can form part of the same WO 03/033012 PCT/GB02/04735 12 cycle; R9 can confer S or R configuration, if applies, to the carbon attached to; and n is 0 to 6. The definitions of the amino acids can also be varied to allow for proline and analogous amino acids including hydroxyproline. The formulae (III), (IV) and can be intermixed to give a side chain made up of repeat units in more than one of these formulae.
In a modification, one or more of the ring amino acids Aaa-6 and Aaaof the hexaamino acid cycle is omitted or an amino acid Aaa-7 is added between Aaa-6 and Aaa-1, in order to arrive at rings having four, five or seven ring amino acids. Six ring amino acids is preferred.
Depending on the type of tumour and the developmental stage of the disease, the treaments of the invention are useful in preventing the risk of developing tumours, in promoting tumour regression, in stopping tumour growth and/or in preventing metastasis.
Administration of the compounds or compositions of the present invention is by intravenous infusion. Infusion times of up to 72 hours can be used, more preferably 1 to 24 hours, with either about 1 or about 3 hours most preferred. Short infusion times which allow treatment to be carried out without an overnight stay in hospital are especially desirable. However, infusion may be around 24 hours or even longer if required.
Although guidance for the dosage is given above, the correct dosage of the compound will vary according to the particular formulation, the mode of application, and the particular situs, host and tumour being treated. Other factors like age, body weight, sex, diet, time of administration, rate of excretion, condition of the host, drug combinations, reaction sensitivities and severity of the disease shall be taken into account. Administration can be WO 03/033012 PCT/GB02/04735 13 carried out continuously or periodically within the maximum tolerated dose.
The Recommended Dose (RD) is the highest dose which can be safely administered to a patient producing tolerable, manageable and reversible toxicity according to the Common Toxicity Criteria (CTC) established for example by the National Cancer Institute, (USA) typically with no more than 2 out of 6 patients presenting any dose limiting toxicities (DLT). Guidelines for cancer therapy frequently call for administration of chemotherapeutic agents at the highest safe dose at which toxicity is manageable in order to achieve maximum efficacy (DeVita, V. T. Jr., Hellman, S. and Rosenberg, S.
Cancer: Principles and Practice of Oncology, 3rd ed., 1989, Lipincott, Philadelphia). For the kahalalide compounds, in particular kahalalide F, the recommended doses are as defined above and set forth in the examples.
The administration is performed in cycles, in the preferred application method, an intravenous infusion of kahalalide compounds given to the patients the first week of each cycle, the patients are allowed to recover for the remainder of the cycle. The preferred duration of each cycle is of either 1, 3 or 4 weeks; multiple cycles can be given as needed. Dose delays and/or dose reductions and schedule adjustments are performed as needed depending on individual patient tolerance of treatments, in particular dose reductions are recommended for patients with higher than normal serum levels of liver transaminases or alkaline phosphatase.
Pharmaceutical compositions of kahalalide compound that can be used include liquid (solutions, suspensions or emulsions) with suitable composition for intravenous administration, and they may contain the pure compound or in combination with any carrier or other pharmacologically active compounds. Further guidance concerning the pharmaceutical compositions WO 03/033012 PCT/GB02/04735 14 can be found in WO 02 36145 which is incorporated herein by reference in its entirety.
Thus, a combination of a non-ionic surfactant and an organic acid is suited for use with a bulking agent to give a lyophilised form of a kahalalide compound suited for reconstitution. Reconstitution is preferably effected with a mix of emulsifying solubiliser, alkanol and water.
The lyophilised composition preferably comprises mainly the bulking agent, such as at least 90 or at least 95 bulking agent. Examples of bulking agents are well known and include sucrose and mannitol. Other bulking agents can be employed.
The non-ionic surfactant in the lyophilised composition is preferably a sorbitan ester, more preferably a polyethylene sorbitan ester, such as a polyoxyethylene sorbitan alkanoate, especially a polyoxyethylene sorbitan mono-oleate, for example polysorbate 80. The non-ionic surfactant typically comprises a few of the composition, such as 0 to 5 of the composition, for instance 2 to 3 or 4 of the composition.
The organic acid in the lyophilised composition is typically an aliphatic acid, preferably a hydroxycarboxylic acid and more preferably a hydroxypolycarboxylic acid, notably citric acid. The organic acid typically comprises a few of the composition, such as 0 to 5 of the composition, for instance 2 to 3 or 4 of the composition.
The amount of kahalalide compound in the lyophilised composition is typically less than 1 or often less than 0.1 of the mix. A suitable amount is in the range 50 to 200 pg, say about 100 pig, per 100 mg of WO 03/033012 PCT/GB02/04735 composition.
The emulsifying solubiliser for the reconstituting agent suitably comprises an polyethylene glycol ester, notably an ester of a fatty acid, more preferably a PEG oleate such as PEG-35 oleate. The emulsifying solubiliser is suitably 0 to 10 of the reconstituting agent, typically about 3 to 7 say about 5 The alkanol is usually ethanol, and is suitably 0 to of the reconstituting agent, typically about 3 to 7 say about 5 The remainder of the reconstituting agent is water, and gives a reconstituted solution suited for intravenous injection.
Further dilution of the reconstituted solution with 0.9 saline may be appropriate for infusion of the kahalalide compound.
In a particularly preferred embodiment, the lyophilised composition comprises 150 ug kahalalide F; 150 mg sucrose; 3 mg anhydrous citiric acid; and 3 mg of polysorbate The preferred reconstituting agent then comprises 2 to 7 say about emulsifying solubiliser; 2 to 7 say about 5 alcohol; and remainder water.
The invention additionally provides kits comprising separate containers containing the lyophilised composition and the reconstituting agent. Methods of reconstitution are also provided.
The present invention further provides a method of treating any mammal, notably a human, affected by cancer which comprises administering to the affected individual a therapeutically effective amount of a WO 03/033012 PCT/GB02/04735 16 pharmaceutical composition thereof prepared by reconstitution of a lyophilised composition of this invention. The present invention can be employed particularly for treatment of patients with refractory cancers that do not respond favourably to other treatments. In particular, the compositions of this invention can be employed after other chemotherapy has been tried and not worked.
In one embodiment, the reconstituted solution is prepared for infusion and is administered in a 3-hour infusion on concentrations of up to around or 25 jg/ml, typically up to 15 jig/ml. Suitable infusion equipment preferably includes a glass container, rather than one of polyethylene.
Tubing is preferably of silicone.
We prefer that infusion times of up to 24 hours are used, and as explained we prefer an infusion time of about 1 hour. In a variation, the infusion time is 2-12 hours, such as 2-6 hours. Short infusion times which allow treatment to be carried out without an overnight stay in hospital are especially desirable. However, infusion may be 12 to 24 hours or even longer if required. Infusion may be carried out at suitable intervals of say 2 to 4 weeks. In an alternative dosing protocol, the kahalalide compound such as kahalalide F is administered for say about 1 hour for 5 consecuvitve days every 3 weeks. Other protocols can be devised as variations.
The compounds and compositions of this invention may be used with other drugs to provide a combination therapy. The other drugs may form part of the same composition, or be provided as a separate composition for administration at the same time or a different time. The identity of the other drug is not particularly limited.
WO 03/033012 PCT/GB02/04735 17 EXAMPLES OF THE INVENTION Clinical trials were carried out based on the following protocols: Number of patients per dose level and Dose escalation The rate of subject entry and escalation to the next dose level will depend upon assessment of the safety profile of patients entered at each dose level. Toxicity will be evaluated and graded according to the Common Toxicity Criteria, version In order to minimize the number of patients treated at the subtoxic dose levels, a single patient will be treated per dose level with 100% dose escalation in the absence of any toxicity G2 (excluding alopecia) during their first course of treatment. After the occurrence of grade 2 toxicity (excluding asthenia, alopecia, nausea and vomiting or anemia), the dose level is expanded to three patients with only 50% incremental increase in dosage in advancing to the next dose level. For the occurrence of grade 3 nonhematological toxic effects the dose level should be expanded up to six patients. This second cohort of 3 patients will be included consecutively. If the first or second patient develops dose limiting toxicities no further patients will be treated at this dose level, it will be defined as MTD and the accrual will begin at a lower dose level. If only one DLT occurs at this dose level another 3 patients will be included at the next dose level with an increase of 25% only.
Observed toxicities Action WO 03/033012 PCT/GB02/04735 <G1 toxicities Treat 1 patient per dose level with 100% dose escalation G2 toxicities Treat 3 pats at that dose level with increase in Dose escalation G3 toxicities non- Expand current dose level up to 6 hematological pats and 25% increase in dose escalation 1/3 or 2 2/6 DLTs MTD Clinical pharmacokinetic data on Kahalalide F will become available during the course of each dose level and will be considered when making a final judgement.
There is no intrapatient dose escalation, only de-escalation. If the toxicity is grade 3 or worse in a course of chemotherapy, then the dose is reduced one level for the next course.
Maximum Tolerated Dose (MTD) and Recommended Dose (RD) for Phase II studies The maximum tolerated dose (MTD) is defined as the least 2 out of 3 or more than 3 patients experience DLT.
possible that additional patients may experience DLT due patient enrollment into that dose level.
dose at which at However, it is to the timing of WO 03/033012 PCT/GB02/04735 19 Once an MTD level is established, subsequent patients should be treated at the next lower dose level. Intermediate doses may be used in some instances and flexibility is an integral part of the protocol.
At the RD, sufficient number of patients will be accrued to further define the toxicity profile at this dose level.
KHF A 001-00 Definition of Dose Limiting Toxicities Dose limiting toxicities (DLTs) are defined as follows: Grade IV Neutropenia lasting for 2 5 days or with a temperature 38.5.
Grade IV thrombocytopenia.
Any other grade III or IV non hematological toxicity, excluding nausea, emesis and hypersensitivity reactions.
Grade III or IV liver toxicity if not reversible by day 21.
These toxicities are only considered DLTs if they happen during cycle 1.
KHF 002-01 Definition of Dose Limiting Toxicities The toxicity will be evaluated and clasified in grades according to the Common Toxicity Criteria (CTC) version NCI 2.0, March 1998.
Dose limiting toxicities (DLTs) are defined as follows: ANC <500/mm3 for more than 5 days.
ANC <500/mm3 with a temperature of 38'5 or more.
Plaquetas 25000/mm3 WO 03/033012 PCT/GB02/04735 Any other non-hematological grade 3-4 toxicity excluding: nausea, emesis without prophilaxis G3 transaminases increase >14 days Hypersensibility reactions Example 1 Preliminary Report Phase I and Pharmacokinetic Study of Kahalalide F in Patients With Advanced Androgen Refractory Prostate Cancer INTRODUCTION: Kahalalide F (KF) is one of a family of novel dehydroaminobutyric acid-containing peptides isolated from the Hawaiian herbivorous marine species of mollusk, Elysia rufescens. KF displays both in vitro and in vivo anti-tumour activity in various solid tumour models including breast, colon, non-small cell lung, and in particular prostate cancer.
On the basis of its selectivity, KF is now further developed as a potential anticancer agent against androgen independent prostate tumours.
OBJECTIVE: In the present phase I clinical and pharmacokinetic (PK) study the toxicity profiles PK and anti-tumour activity of KF are investigated.
METHODS: KF is administered as an intravenous infusion over one hour, during five consecutive days every three weeks in patients with advanced or metastatic androgen refractory prostate cancer. On the basis of the MTD values defined in mice, a starting dose of 20 g/m2/day was selected, which is equivalent to a total dose of 100 4g/m2. PK of KF were determined in plasma during the first course. Bioanalysis of KF was performed by LC- MS/MS. LDH, AF and especially PSA levels of each patient were also WO 03/033012 PCT/GB02/04735 21 evaluated during the study to determine the activity of KF. RESULTS AND DISCUSSION: At present 7 patients have been registered. Patients had a median age of 66 years (range 54-75). One patient per level was entered at 40, 80 and 160 tg/m2/day. Due to transaminase elevation a number of 4 patients were entered at the current dose level, 320 pg/m2/day. The first patient of this study was re-entered at this dose level. Observed adverse events were rapidly reversible mild headache, fatigue, pain and local edema.
The only drug related toxicity to date was a rapidly reversible CTC grade 3 ASAT that occurred at 320 pg/m2/day. PK revealed a linear relationship between dose and AUC over the whole dose range. Total plasma clearance was 267 mL/min 115) and the terminal half-life of intravenous KF in these patients was 0.46 h 0.13). Maximum plasma concentrations reached at the current dose level (35-50 ng/mL) are potentially active for prostate cancer in the clonogenic tumour assay (activity from 15 ng/mL). Thus far, the schedule is well tolerated. One patient sowed a significant decrease in PSA level associated to clinical improvement (pain relief). Two additional patients experienced minor PSA reductions, one still ongoing after two cycles. The maximum tolerated dose has not been reached yet and the study is ongoing.
Example 2 KHF-A-001-00. Phase I Clinical and Pharmacokinetic study to determine the safety of kahalalide F administered as a daily x 5 over 1 hour Infusion every 21 days in patients with advanced or metastatic prostate cancer.
The first clinical trial with KHF is being run in The Netherlands. It is addressed to prostate cancer patients according to the high selectivity WO 03/033012 PCT/GB02/04735 22 exhibited in the preclinical programme. The toxicity data in rodents and dogs shows the feasibility to give daily doses equivalent to the single dose MTD in a repeated fashion (daily times five): such schedule may significantly enhance the therapeutic profile of KF in patients bearing hormone resistant prostate cancer.
A dose of 20 mcg/m2 was considered a safe starting dose on the basis of acute toxicity studies in animals. The trial was designed as an accelerated dose escalation (pharmacokinetically guided).
One patient (the first one treated in this trial received 4 cycles at the first dose level. 2 months after stopping the treatment, was included at the current dose level and received another 4 cycles. Grade 3 hypertransaminasemia was the reason to expand the cohort in the 5th level.
Level I II III IV V VI Dose 20 40 80 160 320 425 Increase% 100 100 100 100 33 Patient 1 1 1 1 5 3 Cycles 4 1 8 4 2,4,8,2,2 3, 2, 2 Worst none none none none G3 none toxicity Hypertrans Efficacy PR SD Level VII VIII IX X Dose 560 700 930 700 Increase% 33 25 33 Patient 3 3 3 2 Cycles 4, 2, 2 2,2,2 5, 3, 2 WO 03/033012 PCT/GB02/04735 23 Worst G3 none G4 toxicity Hypertrans Hypertrans Hypersensitivity Efficacy SD patients and 66 cycles could be evaluated by August, 2002.
HEMATOLOGIC TOXICITY (worst per patient) 19 patients Grade 1 Grade 2 Grade 3 Neutrophils 5 5 0 Leukocytes 10.5 0 0 Platelets 21.1 0 0 Hemoglobin 63.2 26.3 0 Grade 4 0 0 0 0 NON-HEMATOLOGIC TOXICITY patients Grade 1 Creatinine 35 Bilirubin 10 Alk.phosphatase 35 GOT 40 GPT 15 GGT 35 HypoNa+ 10 HypoK+ 20 HypoCa* 65 Hypo P 10 Albumin 30 CPK 35 *Calcium adjusted LAB ABNORMALITIES per Grade 2 Grade 3 0 0 0 0 20 25 15 15 25 20 15 25 0 5 0 0 0 0 15 0 10 5 0 0 patient Grade 4 0 0 0 0 0 0 0 WO 03/033012 PCT/GB02/04735 24 Most frequently reported NON-HEMATOLOGIC TOXICITIES patients Grade 1 Grade 2 Grade 3 Grade 4 Diarrhea 10 5 0 0 Fatigue 35 10 25 0 Nausea 15 10 0 0 Hypersensitivity 20 5 0 0 Hypertension 0 0 15 0 Inject-site 10 20 0 0 reaction Periph-sens 20 0 5 0 neuro Vomiting 15 10 0 0 From preclinical studies renal and neurologic were the most expected toxicities, but surprisingly none of them has constituted remarkable toxicity.
However, liver toxicity was the most commonly seen as grade 4 transaminases elevation in the MTD (GOP, GPT levels). Patients also complain of puncture or prick on hands palms during the infusion.
Hypertension and alkaline phosphatase were not drug-related.
As it was predicted by preclinical experiments no hematological toxicity was seen. No grade 3-4 emesis was reported.
Regarding efficacy, one patient was evaluated as a partial response (evaluated by PSA). This patient received 8 cycles. Two more patients exhibited SD (they were also evaluated by PSA) as best response and received 4 cycles. All these patients concomitantly showed clinical WO 03/033012 PCT/GB02/04735 improvement.
Example 3 KHF-A-002-01. Phase I Clinical and Pharmacokinetic study to determine the safety of Kahalalide F administered as a weekly infusion over 1 hour in patients with solid tumours.
This trial was addressed to any solid tumours and was designed as a classical escalation. The starting dose was higher because we had some information from the first trial, and this allowed us to skip the first steps.
One cycle in this trial means a week, so 4 cycles mean 1 month of treatment.
The second dose level (400) was expanded because of two unrelated adverse events: grade 3 diarrhea and death due to gastrointestinal bleeding.
This was also the reason why the next escalation was only 32.5% instead of No toxicities were reported in this level and the following escalation was 50% again.
The DLT was identified at 1200 mcg/m2, and was grade 4 hypertransaminasemia non reversible by day 21. The time of onset was located at 5 hours after the infusion. This was not a scheduled determination and that was why the dose was descalated looking for previously unidentified grade 4 hypertransaminasemia. The 4th level was reexplored and this second time, grade 4 hypertransaminasemia was identified as DLT again at this dose level.
19 patients and 145 cycles were evaluated.
WO 03/033012 PCT/GB02/04735 Level Dose Increase% Patient Cycles I II 266 400
III
530
IV
800 3 10,11,8 3 5,8,8 6 2,6,7, 9,21,4 3 7,8,16 1200 8,8,3,8 Hypersensitivity hypertrans Worst toxicity Efficacy Cavum Hepatocell
NSCLC
Level VI VII VIII Dose 1000 800 650 Increase% Patient 3 3 Cycles 3,1,3 2,1,1 Worst Hypersensitivity G4 toxicity hypertrans Hypertrans Efficacy Patients have complained of puncture or prick on hands palms in all the dose levels.
One SAE with hypersensitivity reactions was reported. The patient had both pruritus and bronchoconstriction after the first cycle at 1200 mcg/sm. He could not be retreated in spite of administering standard premedication.
WO 03/033012 PCT/GB02/04735 OTHER TOXICITIES HEMATOLOGIC TOXICITY (worst Grade 1 Neutrophils 5.3 Leukocytes 15.8 Platelets 10.5 Hemoglobin 57.9 per patient) Grade 2 5.3 5.3 0 21.1 Grade 3 0 0 0 0 Grade 4 0 0 0 0 NONHEMATOLOGIC TOXICITY. LABORATORY ABNORMALITIES Creatinine Bilirubin Alk.phosphatase
GOT
GPT
GGT
Na+ hypo K+ hypo Ca+ hypo Ca+ hyper
CPK
Grade 1 31.6 5.6 27.8 38.9 22.2 16.7 21.1 5.3 21.1 84.2 36.8 Grade 2 5.3 0 11.1 0 5.6 11.1 0 0 0 0 0 Grade 3 0 0 0 0 0 5.6 0 0 0 0 0 Grade 4 0 0 0 16.7 16.7 11.1 5.3 0 0 0 0 Most frequently reported NON-HEMATOLOGIC patients Grade 1 Grade 2 Diarrhea 0 5.3 Fatigue 21.1 21.1
TOXICITIES
Grade 3 5.3 0 Grade 4 0 0 WO 03/033012 PCT/GB02/04735 28 Nausea 31.6 10.5 0 0 Hypersensitivity 15.8 5.3 5.3 0 Hypertension 0 0 0 0 Inject-site 0 5.3 0 0 reaction Periph-sens 36.8 0 5.3 0 neuro Vomiting 10.5 5.3 0 0
EFFICACY
Some hints of efficacy were seen in this trial, in a patient with a epidermoid carcinoma of the cavum, treated at 400 mcg/m2. Subjective improvement and non evaluable response (ORL exploration). Another patient with hepatocellular carcinoma, treated at 400 mcg/m2. He received the treatment during 6 months (24 cycles). Partial response in one target lesion (overall response: stable disease). Also in a patient with non small cell lung cancer, treated at 530 mcg/m2. He received the treatment during 4 months (16 infusions) without evidence of progression disease. One patient with peritoneal mesothelioma had clinical benefit with reduction of ascytes volume.
HEMATOLOGIC TOXICITY KHF was supposed to be a non myelosuppressor agent. This prediction seems to have been confirmed in the clinical programme. Grade 1-2 neutropenia was seen in 10% of patients. No grade 3-4 toxicities were WO 03/033012 PCT/GB02/04735 29 reported.
RENAL TOXICITY This was an expected toxicity for KHF since it was dose limiting toxicity in the preclinical programme. However renal toxicity has not been a relevant toxicity. Only about 35% of patients exhibited grade 1 renal toxicity. Since this renal toxicity was expected to be more tubular than glomerular, we have carefully reviewed seric ions. Grade 1 hypokalyemia occurred in the 20 of patients, grade 1 and 2 hypophosphatemia in 10 and Grade 1 hypocalcemia was a frequent feature in the first trial (patients with prostate cancer) but it was not so frequent in patients treated in the second trial. Ions loss in urine should be confirmed.
HYPERTRANSAMINASEMIA
It has been the DLT for KHF. It is clearly dose-related. At 800- 1200 mcg/m2/wk all the patients had grade 4 hypertransaminasemia.
Hypertransaminasemia peaks 4-5 hours after the infusion of KHF and is not reversible by day 8 for patients treated at 1000 and 1200. One patient showed LDH elevation too. Neither bilirubin nor alkaline phosphatase elevations were reported. Patients remained asymptomatic. This toxicity was completely reversible.
Though transaminases are found in other organs, a grade 4 ALT is a marker of hepatocellular injury.
HIPERSENSITIVITY
Probably the most frequent toxicity observed with KHF is the palm hand prick referred by patients while the infusion. Sometimes this complaint was accompanied by other symptoms like erythema or pruritus in other locations.
Bronchoconstriction was observed in two patients at the highest dose level (1000 and 1200). Pruritus with redness and sometimes facial edema was seen. It could be more a toxic effect than an immune mediated (hypersensitivity reaction) sine it was more commonly reported in the highest levels.
CONCLUSIONS
In this second example dose limiting toxicity for Kahalalide F was grade 4 hypertransaminasemia as in the previous example with a different schedule. It is an asymptomatic and reversible feature that appears 4-5 hours after the infusion of KHF. Hypersensitivity reactions with cutaneous features were commonly reported at highest levels.
Lack of hematologic toxicity was confirmed as it was predicted by preclinical studies.
In the claims which follow and in the preceding description of the invention, except where the context requires otherwise due to express language or necessary implication, the word "comprise" or variations such as "comprises" or "comprising" is used in an inclusive sense, i.e. to specify the presence of the stated features but not to preclude the presence or addition of further features in various embodiments of the invention.
It is to be understood that, if any prior art publication is referred to herein, such reference does not constitute an admission that the publication forms a part of the common general knowledge in the art, in Australia or any other country.
N:\Melbourne\Cases\Patent\520OO52999\P52651.AU\SpeCia\P52651.AUdoC 29/06/07
Claims (39)
1. Use of kahalalide F in the preparation of a medicament for treating a human which comprises administering kahalalide F at a dose of at least 320 mcg/n 2 /day.
2. A use according to claim 1, comprising administering kahalalide F at a dose below 1200 mcg/m 2 /day.
3. A use according to claim 2, wherein the dose is below 930 mcg/m 2 /day.
4. A use according to claim 2, wherein the dose is below 800 mcg/m 2 /day. A use according to claim 1, wherein the dose is in the range of 400-900 mcg/m 2 /day.
6. A use according to claim 5, wherein the dose is 500-800 mcg/m 2 /day.
7. A use according to claim 6, wherein the dose is 600-750 mcg/n 2 /day.
8. A use according to claim 7, wherein the dose is about 650-700 mcg/m2/day.
9. Use of kahalalide F in the manufacture of a medicament for treating a human patient, which comprises administering to said patient kahalalide F daily during 5 days at a dose below 930 mcg/mn/day, followed by a resting period of fi-om 1 to 4 weeks in which kahalalide F is not administered. A use according to claim 9, wherein the dose is 650-750 mcg/m 2 /day. S1. A use according to claim 9, wherein the dose is about 700 mcg/m 2 /day.
12. A use according to claim 9, wherein the dose is 560 mcg/m 2 /day.
13. A use according to any one of claims 9 to 12, wherein the infusion time is 1 to 24 hours. 13/12 2007 15:13 FAX 61 3 92438333 GRIFFITH HACK IPAUSTRALIA 1A006 -32- 0
14. A use according to claim 13, wherein the infusion time is 1 to 3 hours. 0
15. A use according to claim 14, wherein the infusion time is about 1 hour.
16. A use according to any one of claims 9 to 15, wherein the resting period is 2-3 weeks. S17. A use according to claim 16, wherein the resting time is about 2 weeks. n o10 18. Use of kahalalide F in the manufacture of a medicament for treating a human Cpatient, which comprises administering to said patient kahalalide F once weekly o at a dose below 800 mcg/m 2 /day. ci
19. A use according to claim 18 wherein the dose is 600-700 mcg/m 2 /day. A use according to claim 19, wherein the dose is about 550 mcg/mn2/day.
21. A use according to any one of claims 18 to 20, wherein the infusion time is 1 to 24 hours.
22. A use according to claim 21, wherein the infusion time is 1 to 3 hours.
23. A use according to claim 22, wherein the infusion time is about 1 hour.
24. A use according to any one of claims 1 to 23, wherein the human has a metastatic tumour. A use according to any one of claims 1 to 23, wherein the human has an advanced solid tumour.
26. A use according to any one of claims 1 to 25, wherein -he human has a hormone independent prostate cancer.
27. A use according to any one of claims 1 to 25, wherein the human has hepatocarcinoma, epithelial carcinoma, non small cell lung cancer or mesothelioma. N;Melbourfn MBl5e2tPl>t -520-999fW2S 265I A Specs,9P5265 IAU Spcflcdon 2007-12-4Aoc 13/12/07 COMS ID No: ARCS-172267 Received by IP Australia: Time 16:12 Date 2007-12-13 13/12 2007 15:13 FAX 61 3 92438333 GRIFFITH HACK 4 IPAUSTRALIA I007 33 0
28. A method of treating a human with kahalalide F which comprise administering C. at least 320 mcg/m 2 /day. en 29. A method according to claim 28, comprising administering kahalalide F at a dose below 1200 mcg/m 2 /day. Mn 30. A method according to claim 29, wherein the dose is below 930 mcg/m /day.
31. A method according to claim 29, wherein the dose is below 800 mcg/m 2 /day.
32. A method according to claim 28, wherein the dose is in the range of 400-900 Smcg/m 2 /day.
33. A method according to claim 32, wherein the dose is 5C 0-800 mcg/m 2 /day.
34. A method according to claim 33, wherein the dose is 600-750 mcg/m 2 /day. A method according to claim 34, wherein the dose is abut 650-700 mcg/m 2 /day.
36. A method for treating a human patient comprising administering to said patient kahalalide F daily during 5 days at a dose below 930 mcg/m 2 /day, followed by a resting period of from 1 to 4 weeks in which kahalalide F is not administered.
37. A method according to claim 36, wherein the dose is 650-750 mcg/m 2 /day.
38. A method according to claim 36, wherein the dose is 700 mcg/m /day.
39. A method according to claim 36, wherein the dose is 560 mcg/m 2 /day.
40. A method according to any one of claims 36 to 39, wherein the infusion time is 1 to 24 hours.
41. A method according to claim 40, wherein the infusion time is 1 to 3 hours. 3s 42. A method according to claim 41, wherein the infusion time is about 1 hour.
43. A method according to any one of claims 36 to 42, wherein the resting period is N:\MalbouhelTCase.Malel52000-.52999P52653 AUSpectsP52651.AU Specifintion200-12-4doc 13112/07 COMS ID No: ARCS-172267 Received by IP Australia: Time 16:12 Date 2007-12-13 I -34- 0 2-3 weeks. S44. A method according to claim 43, wherein the resting time is about 2 weeks. 00 5 45. A method of treating a human patient which comprises administering to said patient a kahalalide F compound once weekly at a dose below 800 mcg/m 2 /day. S46. A method according to claim 45, wherein the dose is 600-700 mcg/m 2 /day. C1)
47. A method according to claim 45, wherein the dose is about 650 mcg/m 2 /day.
48. A method according to any one of claims 45 to 47, wherein the infusion time is 1 to 24 hours.
49. A method according to claim 48, wherein the infusion time is 1 to 3 hours. A method according to claim 49, wherein the infusion time is about 1 hour.
51. A method according to any one of claims 28 to 50, wherein the human has a metastatic tumour.
52. A method according to any one of claims 28 to 50, wherein the human has advanced solid tumour.
53. A method according to any one of claims 28 to 52, wherein the human has a hormone independent prostate cancer.
54. A method according to any one of claims 28 to 52, wherein the human has hepatocarcinoma, epithelial carcinoma, non small cell lung cancer or mesotheliona. Uses of kahalalide F and/or methods of treatment involving kahalalide F substantially as herein described with reference to the examples herein. N:\Mlbourme\Cases\P'att\52000.52999\P5265 I.A ULSpecis\PS265 AU Specification 2007- 2-4.doc 17/04/08
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US34844901P | 2001-10-19 | 2001-10-19 | |
US60/348,449 | 2001-10-19 | ||
PCT/GB2001/004821 WO2002036145A2 (en) | 2000-10-31 | 2001-10-31 | Kahalalide f formulation |
AUPCT/GB2001/004821 | 2001-10-31 | ||
GB0222409.5 | 2002-09-26 | ||
GB0222409A GB0222409D0 (en) | 2002-09-26 | 2002-09-26 | Use of antitumoral compounds in cancer therapy |
PCT/GB2002/004735 WO2003033012A1 (en) | 2001-10-19 | 2002-10-18 | Kahalalide compounds for use in cancer therapy |
Publications (3)
Publication Number | Publication Date |
---|---|
AU2002334203A1 AU2002334203A1 (en) | 2003-07-03 |
AU2002334203B2 AU2002334203B2 (en) | 2008-01-24 |
AU2002334203C1 true AU2002334203C1 (en) | 2008-10-09 |
Family
ID=27256084
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2002334203A Ceased AU2002334203C1 (en) | 2001-10-19 | 2002-10-18 | Kahalalide compounds for use in cancer therapy |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1435990A1 (en) |
AU (1) | AU2002334203C1 (en) |
CA (1) | CA2462639A1 (en) |
WO (1) | WO2003033012A1 (en) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR0315489A (en) * | 2002-10-18 | 2005-08-23 | Pharma Mar Sau | Antitumor Composition |
GB0304367D0 (en) | 2003-02-26 | 2003-04-02 | Pharma Mar Sau | Methods for treating psoriasis |
SI1572726T1 (en) * | 2002-10-18 | 2011-04-29 | Pharma Mar Sau | 4-methylhexanoic kahalalide f compound |
US7507708B2 (en) | 2003-02-26 | 2009-03-24 | Pharma Mar, S.A.U. | Antitumoral compounds |
GB0321066D0 (en) | 2003-09-09 | 2003-10-08 | Pharma Mar Sau | New antitumoral compounds |
GB0408958D0 (en) * | 2004-04-22 | 2004-05-26 | Pharma Mar Sa | Convergent synthesis for kahalalide compounds |
JP2011515330A (en) * | 2008-01-30 | 2011-05-19 | ファルマ・マール・ソシエダード・アノニマ | Improved antitumor treatment |
JOP20190254A1 (en) | 2017-04-27 | 2019-10-27 | Pharma Mar Sa | Antitumoral compounds |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9302046D0 (en) * | 1993-02-03 | 1993-03-24 | Pharma Mar Sa | Antiumoral compound-v |
KR100834138B1 (en) * | 2000-10-31 | 2008-06-02 | 파르마 마르, 에스.에이. | Kahalalide f |
-
2002
- 2002-10-18 WO PCT/GB2002/004735 patent/WO2003033012A1/en not_active Application Discontinuation
- 2002-10-18 AU AU2002334203A patent/AU2002334203C1/en not_active Ceased
- 2002-10-18 CA CA002462639A patent/CA2462639A1/en not_active Abandoned
- 2002-10-18 EP EP02801430A patent/EP1435990A1/en not_active Ceased
Non-Patent Citations (1)
Title |
---|
Nuijen et al., MEDLINE, XP002206588 * |
Also Published As
Publication number | Publication date |
---|---|
WO2003033012A1 (en) | 2003-04-24 |
EP1435990A1 (en) | 2004-07-14 |
AU2002334203B2 (en) | 2008-01-24 |
CA2462639A1 (en) | 2003-04-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US10758556B2 (en) | Anthracycline-based antibody drug conjugates having high in vivo tolerability | |
ES2315278T3 (en) | IMMUNOR REGULATOR. | |
CN101240013B (en) | High purity lipopeptides, lipopeptide micelles, processes for preparing same | |
JP5559087B2 (en) | Methods and compositions for the treatment of gastrointestinal disorders | |
CN1964736B (en) | Dalbavancin compositions for treatment of bacterial infections | |
CN109912683A (en) | A kind of cytotoxic molecule, conjugate and its preparation method and application | |
CN105308065A (en) | Polymyxin derivatives and their use in combination therapy together with different antibiotics | |
JP2010047601A (en) | Kahalalide f | |
Newman et al. | VRCTC-310—a novel compound of purified animal toxins separates antitumor efficacy from neurotoxicity | |
AU2002334203C1 (en) | Kahalalide compounds for use in cancer therapy | |
US20080090757A1 (en) | Kahalalide Compositions for the Treatment of Psoriasis | |
US20050054555A1 (en) | Kahalalide compounds for use in cancer therapy | |
AU2002334203A1 (en) | Kahalalide compounds for use in cancer therapy | |
ES2377635T3 (en) | Analogous peptide analogous peptide derivative compounds Crotalus durissus terrificus, its uses, compositions, preparation and purification procedures | |
US20210130403A1 (en) | Inhibitors of metastasis | |
US20180251495A1 (en) | Skin-Penetrating Peptides and Compositions and Methods of Use Thereof | |
MXPA04003672A (en) | Kahalalide f formulation. | |
KR20030077950A (en) | Physiologically active complex | |
US20230053129A1 (en) | Compound for the treatment of the hemolytic-uremic syndrome | |
RU2292216C2 (en) | Kahalalide f | |
CZ159298A3 (en) | Pharmaceutical preparation | |
TW202345904A (en) | Ligand-drug-conjugates with improved pharmacokinetic and drug release properties | |
KR20190135344A (en) | Fusion Protein Comprising Protein Derived from Lactic Acid Bacteria and Anti-Cancer Peptide and Use of the Same |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
DA3 | Amendments made section 104 |
Free format text: THE NATURE OF THE AMENDMENT IS: AMEND THE NAME OF THE CO-INVENTOR FROM LOPEZ, LAZARO LUIS TO LOPEZ LAZARO, LUIS |
|
DA2 | Applications for amendment section 104 |
Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 18 APR 2008. |
|
FGA | Letters patent sealed or granted (standard patent) | ||
DA3 | Amendments made section 104 |
Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 18 APR 2008 |
|
MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |