AU2001295592A1 - Method for synthesising peptides, peptide mimetics and proteins - Google Patents

Method for synthesising peptides, peptide mimetics and proteins

Info

Publication number
AU2001295592A1
AU2001295592A1 AU2001295592A AU9559201A AU2001295592A1 AU 2001295592 A1 AU2001295592 A1 AU 2001295592A1 AU 2001295592 A AU2001295592 A AU 2001295592A AU 9559201 A AU9559201 A AU 9559201A AU 2001295592 A1 AU2001295592 A1 AU 2001295592A1
Authority
AU
Australia
Prior art keywords
proteins
enzyme
peptide mimetics
synthesising peptides
peptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001295592A
Inventor
Hans-Dieter Jakubke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of AU2001295592A1 publication Critical patent/AU2001295592A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21004—Trypsin (3.4.21.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The method for producing peptides, peptide mimetics and proteins is characterized in that an enzyme is used as a biocatalyst together with a peptide mimetic. The native specificity of the enzyme is modified by chemical or genetic manipulation, the substrate mimetic is a carboxyl component with an ester leaving group whose specificity determinant is adapted to the recognition site of the enzyme. The invention also concerns the trypsin variant D189K,K60E and its use as a C-N ligase in segment condensations.
AU2001295592A 2000-10-07 2001-10-05 Method for synthesising peptides, peptide mimetics and proteins Abandoned AU2001295592A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10049673.3 2000-10-07
DE10049673A DE10049673A1 (en) 2000-10-07 2000-10-07 Enzymatic synthesis of peptide, its mimic or protein, useful e.g. in drug development, using a modified ligase adapted to a particular ester leaving group
PCT/EP2001/011500 WO2002031177A2 (en) 2000-10-07 2001-10-05 Method for synthesising peptides, peptide mimetics and proteins

Publications (1)

Publication Number Publication Date
AU2001295592A1 true AU2001295592A1 (en) 2002-04-22

Family

ID=7658978

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001295592A Abandoned AU2001295592A1 (en) 2000-10-07 2001-10-05 Method for synthesising peptides, peptide mimetics and proteins

Country Status (9)

Country Link
US (1) US7459526B2 (en)
EP (1) EP1326995B8 (en)
JP (1) JP3892397B2 (en)
AT (1) ATE351917T1 (en)
AU (1) AU2001295592A1 (en)
CA (1) CA2423294C (en)
DE (2) DE10049673A1 (en)
ES (1) ES2278787T3 (en)
WO (1) WO2002031177A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7335504B2 (en) 2003-06-18 2008-02-26 Direvo Biotechnology Ag Engineered enzymes and uses thereof
JP4538501B2 (en) 2004-08-13 2010-09-08 エフ．ホフマン−ラ ロシュ アーゲー C-terminal modification of polypeptides
JP5749744B2 (en) 2010-02-11 2015-07-15 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ IGF-I poly (ethylene glycol) conjugate
CN105296452B (en) * 2015-10-15 2018-10-26 天津大学 A kind of saltant type Sus scrofa pigs source trypsase and its encoding gene and acquisition methods and application

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2087342C (en) 1990-08-09 2003-12-23 Lars Abrahmsen Serine protease variants having peptide ligase activity
AU6171594A (en) 1993-02-04 1994-08-29 Genentech Inc. Serine protease variants having peptide ligase activity

Also Published As

Publication number Publication date
CA2423294C (en) 2008-02-19
JP2004511234A (en) 2004-04-15
DE50111910D1 (en) 2007-03-08
WO2002031177A2 (en) 2002-04-18
EP1326995B8 (en) 2007-02-28
WO2002031177A3 (en) 2003-03-13
DE10049673A1 (en) 2002-04-25
EP1326995B1 (en) 2007-01-17
CA2423294A1 (en) 2002-04-18
JP3892397B2 (en) 2007-03-14
ATE351917T1 (en) 2007-02-15
US20040034197A1 (en) 2004-02-19
US7459526B2 (en) 2008-12-02
ES2278787T3 (en) 2007-08-16
EP1326995A2 (en) 2003-07-16

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