AU2001280201A1 - Methods of synthesizing cell-free protein - Google Patents

Methods of synthesizing cell-free protein

Info

Publication number
AU2001280201A1
AU2001280201A1 AU2001280201A AU2001280201A AU2001280201A1 AU 2001280201 A1 AU2001280201 A1 AU 2001280201A1 AU 2001280201 A AU2001280201 A AU 2001280201A AU 2001280201 A AU2001280201 A AU 2001280201A AU 2001280201 A1 AU2001280201 A1 AU 2001280201A1
Authority
AU
Australia
Prior art keywords
methods
free protein
synthesizing cell
synthesizing
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU2001280201A
Other versions
AU2001280201B2 (en
Inventor
Yaeta Endo
Tomio Ogasawara
Tatsuya Sawasaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CellFree Sciences Co Ltd
Original Assignee
CellFree Sciences Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CellFree Sciences Co Ltd filed Critical CellFree Sciences Co Ltd
Priority claimed from PCT/JP2001/007356 external-priority patent/WO2002024939A1/en
Publication of AU2001280201A1 publication Critical patent/AU2001280201A1/en
Assigned to CELLFREE SCIENCES CO., LTD. reassignment CELLFREE SCIENCES CO., LTD. Request for Assignment Assignors: ENDO, YAETA
Application granted granted Critical
Publication of AU2001280201B2 publication Critical patent/AU2001280201B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

AU2001280201A 2000-08-29 2001-08-28 Methods of synthesizing cell-free protein Ceased AU2001280201B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2000259186 2000-08-29
JP2000-259186 2000-08-29
PCT/JP2001/007356 WO2002024939A1 (en) 2000-08-29 2001-08-28 Methods of synthesizing cell-free protein

Publications (2)

Publication Number Publication Date
AU2001280201A1 true AU2001280201A1 (en) 2002-06-20
AU2001280201B2 AU2001280201B2 (en) 2006-07-13

Family

ID=18747392

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2001280201A Ceased AU2001280201B2 (en) 2000-08-29 2001-08-28 Methods of synthesizing cell-free protein
AU8020101A Pending AU8020101A (en) 2000-08-29 2001-08-28 Methods of synthesizing cell-free protein

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU8020101A Pending AU8020101A (en) 2000-08-29 2001-08-28 Methods of synthesizing cell-free protein

Country Status (10)

Country Link
US (1) US6869774B2 (en)
EP (1) EP1316617B1 (en)
JP (1) JP3768190B2 (en)
KR (1) KR100749053B1 (en)
CN (2) CN101092446A (en)
AT (1) ATE368747T1 (en)
AU (2) AU2001280201B2 (en)
CA (1) CA2420984A1 (en)
DE (1) DE60129717D1 (en)
WO (1) WO2002024939A1 (en)

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CN1252261C (en) * 2000-08-30 2006-04-19 株式会社无细胞科学 Design and construction of transcription template for synthesizing cell-free protein, and dilution batch-type method of synthesizing cell-free wheat germ protein
JP2002125693A (en) * 2000-10-19 2002-05-08 Toyobo Co Ltd Cell extract composition for synthesizing cell-free protein
JP2006129702A (en) * 2002-12-05 2006-05-25 Zoegene Corp Method for producing protein
JPWO2004070047A1 (en) * 2003-02-10 2006-05-25 株式会社セルフリーサイエンス Automatic protein synthesis method and apparatus for performing the same
JP2006042601A (en) * 2003-04-25 2006-02-16 Yaeta Endo High throughput synthetic system
CN1777674B (en) * 2003-04-25 2012-05-23 株式会社无细胞科学 High throughput protein synthetsizing system and synthesizing device for operating same
JP2005027516A (en) * 2003-07-08 2005-02-03 Toyobo Co Ltd Method for synthesizing cell-free protein with continuous energy supply system utilizing intracellular component
US20060188947A1 (en) * 2003-08-07 2006-08-24 Yaeta Endo Reagent for producing a protein chip
WO2005030954A1 (en) * 2003-09-25 2005-04-07 Cellfree Sciences Co.,Ltd. Process for producing antigenic substance
WO2005123936A1 (en) * 2004-06-15 2005-12-29 Zoegene Corporation Method of synthesizing polypeptide
DE102004032460B3 (en) * 2004-06-30 2005-08-18 Rina-Netzwerk Rna Technologien Gmbh In vitro synthesis of expression products, particularly proteins, in a cell-free system, comprises using a repetitive batch method, with periodic removal of metabolites and supplementation
US20060233789A1 (en) * 2004-09-24 2006-10-19 Cellfree Science Co., Ltd. Process for producing antigenic substance
JP4513495B2 (en) * 2004-10-15 2010-07-28 株式会社島津製作所 Cell-free protein synthesis method using insect cell-derived extract
JP2008029204A (en) * 2004-11-12 2008-02-14 Cellfree Sciences Co Ltd Method for synthesizing cell-free protein
KR100730875B1 (en) * 2004-12-07 2007-06-20 가부시키가이샤 시마즈세이사쿠쇼 DNA fragment to promote translation reaction and method for cell-free system protein synthesis using the same
DE102005013608A1 (en) * 2005-03-24 2006-09-28 Westfälische Wilhelms-Universität Münster Apparatus and method for cell-free analytical and preparative protein synthesis
JP2007199047A (en) 2005-12-28 2007-08-09 Cellfree Sciences Co Ltd Method for preparing biotinylated protein, and detecting method using the protein
KR100966655B1 (en) 2007-08-30 2010-06-30 (주)차바이오앤디오스텍 Methods and kits for screening activities of transaminases by using cell-free protein synthesis
US8828986B2 (en) 2011-04-20 2014-09-09 Prosetta Antiviral Inc. Antiviral compounds
US8785434B2 (en) 2010-04-30 2014-07-22 Prosetta Antiviral Inc. Antiviral compounds
US8759336B2 (en) 2011-03-17 2014-06-24 Prosetta Antiviral Inc. Antiviral compounds
US8834694B2 (en) * 2010-12-27 2014-09-16 Mo Bio Laboratories, Inc. Dry compositions and methods for gel electrophoresis
US20120301904A1 (en) * 2011-04-26 2012-11-29 Prosetta Antiviral, Inc Multiprotein assemblies
JP5924502B2 (en) 2011-11-28 2016-05-25 国立大学法人名古屋大学 Biomarker of lymphocytic funnel pituitary pharyngitis and use thereof
CA2891291A1 (en) 2012-11-12 2014-05-15 Moontunes, Inc. Systems and methods for communicating a live event to users using the internet
CN104884606B (en) * 2012-11-30 2020-07-10 株式会社百奥尼 Equipment for automatically preparing cell-free protein and method for preparing protein by using equipment
WO2014144583A2 (en) 2013-03-15 2014-09-18 Northwestern University Methods for cell- free protein synthesis
KR101671688B1 (en) 2014-06-12 2016-11-03 충남대학교산학협력단 Method of cell-free protein synthesis regulating pH with enzymes
CN105219821A (en) * 2015-10-30 2016-01-06 浙江理工大学 The method of exoprotein is synthesized in a kind of microcapsule of alginate composition
US11898187B2 (en) 2017-08-15 2024-02-13 Northwestern University Protein glycosylation sites by rapid expression and characterization of N-glycosyltransferases
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
WO2019204346A1 (en) 2018-04-16 2019-10-24 Northwestern University METHODS FOR CO-ACTIVATING IN VITRO NON-STANDARD AMINO ACID (nsAA) INCORPORATION AND GLYCOSYLATION IN CRUDE CELLLYSATES
WO2020040840A2 (en) 2018-06-01 2020-02-27 Northwestern University Expanding the chemical substrates for genetic code reprogramming
KR102284067B1 (en) * 2019-07-19 2021-07-30 충남대학교 산학협력단 Screening method based on cell-free protein synthesis to determine the factors affecting the expression efficiency or activity of recombinant proteins
CA3158106A1 (en) * 2019-11-15 2021-05-20 David MARASH Methods and systems for generating biological molecules
CN113308580A (en) * 2021-06-18 2021-08-27 江苏支点生物科技有限公司 High-flux cell-free protein synthesis method

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US5478730A (en) * 1988-12-21 1995-12-26 Institute Of Protein Research Method of preparing polypeptides in cell-free translation system
JP2891540B2 (en) * 1989-07-31 1999-05-17 インスティテュト、ベルカ、ロシイスコイ、アカデミイ、ナウク Method for producing polypeptide in cell-free translation system
JPH04200390A (en) * 1990-11-30 1992-07-21 Shigeyuki Yokoyama Production polypeptide by cell-free polypeptide synthesis system
WO1994006928A1 (en) * 1992-09-24 1994-03-31 The Public Health Research Institute Of The City Of New York, Inc. Coupled replication-translation methods and kits for protein synthesis
JP3255784B2 (en) * 1994-01-26 2002-02-12 弥重太 遠藤 Method of protein synthesis
JPH1080295A (en) 1996-07-15 1998-03-31 Nippon Flour Mills Co Ltd Synthesis of protein by cell-free protein synthetic system and apparatus therefor
ATE258592T1 (en) * 1997-07-31 2004-02-15 Roche Diagnostics Gmbh METHOD AND DEVICE FOR CARRYING OUT BIOCHEMICAL REACTIONS
CA2373057A1 (en) * 1999-05-11 2000-11-16 Wakenyaku Co., Ltd. Preparation containing cell extracts for cell-free protein synthesis and means for synthesizing protein using the preparation
EP1221481A4 (en) * 1999-10-15 2004-12-08 Yaeta Dr Endo Template molecule having broad applicability and highly efficient function means of cell-free synthesis of proteins by using the same

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