AU2001279235A1 - Stimulating neutrophil function to treat inflammatory bowel disease - Google Patents
Stimulating neutrophil function to treat inflammatory bowel diseaseInfo
- Publication number
- AU2001279235A1 AU2001279235A1 AU2001279235A AU2001279235A AU2001279235A1 AU 2001279235 A1 AU2001279235 A1 AU 2001279235A1 AU 2001279235 A AU2001279235 A AU 2001279235A AU 2001279235 A AU2001279235 A AU 2001279235A AU 2001279235 A1 AU2001279235 A1 AU 2001279235A1
- Authority
- AU
- Australia
- Prior art keywords
- disease
- patient
- crohn
- agonist
- stimulating factor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Description
STIMULATING NEUTROPHIL FUNCTION TO TREAT INFLAMMATORY BOWEL DISEASE
This application claims the benefit of provisional application Serial No. 60/119,842 filed February 12, 1999. The disclosure of the provisional application is expressly incorporated by reference herein.
BACKGROUND OF THE INVENTION
Crohn's disease persists as an enigma: without a deciphered etiology and without adequate therapy. Prevailing explanations of the pathogenesis of Crohn's disease (Crohn's Disease) hold that the characteristic chronic intestinal inflammation results from an aberrant, activated immune response generated against ubiquitous bacteria or bacterial products that gain access to the lamina propria, perhaps through a more permeable intestinal barrier. The abnormal reaction has been suggested to be mediated principally by T-cells enhanced by an intrinsic imbalance in pro-inflammatory and contra-inflammatory mediators. Thus, most therapy aims to counteract thatinflammatory state with increasingly potent and sophisticated immune suppressants.
Current therapy, mostly directed at suppressing the inflammatory process, remains inadequate both for the treatment of flares and maintenance of remission. Steroids can be effective in short term use but produce dependency in a significant proportion of patients. While certain antibiotics appear promising, data are limited.
Thus there is a need in the art for effective method for treating inflammatory bowel diseases.
SUMMARY OF THE INVENTION
It is an object of the invention to provide a method of treating Crohn's
Disease.
It is an object of the invention to provide a method of treating Ulcerative Colitis.
It is another object of the invention to provide a method of treating extrainstestinal manifestations of Ulcerative Colitis or Crohn's disease.
It is still another object of the invention to provide a method of treating pouchitis.
It is yet another object of the invention to treat and reduce the risk of fistula recurrence. These and other obj ects of the invention are provided by one or more of the embodiments described below. In one embodiment a method is provided of treating Crohn's Disease in which an immune stimulatory amount of an agonist of CDl 14 (Gramilocyte Colony Stimulating Factor Receptor (G-CSFR)) is administered to a patient with Crohn's Disease not associated with Glycogen Storage Disease lb. In another embodiment of the invention another method of treating Crohn's
Disease is provided. An immune stimulatory amount of an agonist of CDl 16 (Granulocyte-Macrophage Colony Stimulating Factor Receptor) or CDwl31 is administered to a patient with Crohn's Disease not associated with Glycogen Storage Disease lb. In yet another embodiment of the invention another method is provided of treating Crohn's Disease. An immune stimulatory amount of an agonist of GDI 14 (GranuϊoGyte Colony Stimulating Factor ReceptQr..(G-,CSER)).is administered to a patient with Crohn's Disease not associated with Chronic Granulomatous Disease.
In still another embodiment of the invention a method is provided of treating Crohn' s Disease in which an immune stimulatory amount of an agonist of CD 116
(Granulocyte-Macrophage Colony Stimulating Factor Receptor) or CDwl31 is administered to a patient with Crohn's Disease not associated with Chronic Granulomatous Disease.
In even another embodiment of the invention another method is provided of treating Crohn' s Disease. An immune stimulatory amount of an agonist of CD 114
(Gramilocyte Colony Stimulating Factor Receptor (G-CSFR)) is administered to a patient with Crohn's Disease not associated with a presently characterized and
identifiable specific neutrophil disorder caused by a genetic disease.
In yet another embodiment of the invention another method is provided of treating Crohn's Disease. An immune stimulatory amount of an agonist of CDl 16 (Granulocyte-Macrophage Colony Stimulating Factor Receptor) or CDwl31 is administered to a patient with Crohn's Disease not associated with a presently characterized and identifiable specific neutrophil disorder caused by a genetic disease.
According to another aspect of the invention a method is provided of treating Ulcerative Colitis. An immune stimulatory amount of an agonist of CDl 14 (Granulocyte Colony Stimulating Factor Receptor (G-CSFR)) is administered to a patient with Ulcerative Colitis.
According to another aspect of the invention a method is provided of treating Ulcerative Colitis. An immune stimulatory amount of an agonist of CDl 16 (Granulocyte-Macrophage Colony Stimulating Factor Receptor) or CDwl31 is administered to a patient with Ulcerative Colitis.
Another aspect of the invention is a method of treating extraintestinal manifestations of Ulcerative Colitis. An immune stimulatory amount of an agonist of CD 114 (Granulocyte Colony Stimulating Factor Receptor (G-CSFR)) is administered to a patient with extraintestinal manifestations of Ulcerative Colitis. Another aspect of the invention is a method of treating extraintestinal manifestations of Ulcerative Colitis. An immune stimulatory amount of an agonist of CD 116 (Graimlocyte-Macrophage Colony Stimulating Factor Receptor) or CDwl31 is administered to a patient with extraintestinal manifestations of Ulcerative Colitis. According to still another aspect of the invention a method is provided of treating pouchitis. An immune stimulatory amount of an agonist of CDl 14 (Granulocyte Colony Stimulating Factor Receptor (G-CSFR)) is administered to a patient with pouchitis.
According to still another aspect of the invention a method is provided of treating pouchitis. An immune stimulatory amount of an agonist of CDl 16
(Granulocyte-Macrophage Colony Stimulating Factor Receptor) or CDwl31 is administered to a patient with pouchitis.
According to still another aspect of the invention a method is provided of preventing or reducing the risk of fistula recurrence. An immune stimulatory amount of an agonist of CDl 14 (Granulocyte Colony Stimulating Factor Receptor (G-CSFR)) is administered to a patient with Crohn's disease who has previously had a fistula, whereby the risk of recurrence of a fistula is reduced.
According to still another aspect of the invention a method is provided of preventing or reducing the risk of fistula recurrence. An immune stimulatory amount of an agonist of CDl 16 (Granulocyte-Macrophage Colony Stimulating Factor Receptor) or CDwl31 is administered to a patient with Crohn's disease who has previously had a fistula, whereby the risk of recurrence of a fistula is reduced.
The present invention thus opens a new realm of treatment modalities for inflammatory bowel diseases which have proven refractory to discovery of safe and effective ministrations. Contrary to the prior paradigm in the art of treating inflammatory bowel diseases with immunosuppressive agents, the present invention uses agents known to be immunostimulatory to treat, prevent, and maintain remission of such diseases. BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1 shows the response of 17 patients to a 12-week regimen of therapy with G-CSF. Figure 2 shows the response of patient D during treatment and retreatment.
DETAILED DESCRIPTION OF THE INVENTION
It is a discovery of the present inventors that immune modulatory factors which act at CDl 14, CDl 16, and or CDwl31 can be successfully used to treat various forms of inflammatory bowel disease. These include but are not limited to Crohn's Disease, with or without a presently characterized and identifiable specific neutrophil disorder (such as Glycogen Storage Disease lb or Chronic Granulomatous Diseases) pouchitis, fistulas, extraintestinal manifestations of Crohn's Disease, and Ulcerative Colitis. The Ulcerative Colitis can be of any extent, including proctitis, proctosigmoiditis, left-sided colitis, or pan-colitis. The immune modulatory factor can be any which binds to CDl 14, CDwl31 , or CDl 16, including G-CSF, GM-CSF, IL-3, IL-5, and peptidomimetics or non- peptidomimetics of these factors which induce tyrosine phosphorylation of multiple
signaling proteins, which stimulate primary bone marrow cells to form granulocytic colonies in vitro, and/or which elevate peripheral blood neutrophil counts. Nartograstim, myelopoietins, circularly permuted G-CSF sequences, SB247464 are among the known mimetics of G-CSF. See, McWherter et al., Biochemistry 14:4564-71, 1999; Feng et a!., Biochemistry 14:4553-63, 1999; Tian et al., Science
281:257-59, 1998; andKuwabara et al, Am. J. Physiology 271 :E73-84, 1996. M- CSF may also be used in accordance with the present invention. The agonist may be administered as is known in the art. Typically this will be by subcutaneous injection or intravenous infusion, however other methods such as oral, intraperitoneal, subdermal, and intramuscular administrations can be used. Doses which are delivered may be the same as those which are delivered to stimulate an immune response in humans for other disease purposes. Typically doses of the factors will be between about 0.1 and 100 μg kg of body weight per day. More preferably this will be between about 1.0 and 10 μg kg of body weight per day. Most preferably the dose will be between about 2 and 8 μg/kg of body weight per day.
Corresponding amounts of peptidomimetics and non-peptidomimetics to achieve the same activity can be used. White blood cell counts can be monitored to maintain a value in the range of 10 and 60.
The immune modulatory factors are typically growth factors or colony stimulating factors which affect the growth of hematopoietic cells, particularly myeloid cells, including polymorphonuclear leukocytes, monocytes, and macrophages. Such factors include but are nβtiimited to myelojd cefl timulatory factors, polymorphonuclear leukocyte stimulatory factors, and granulocytic cell stimulatory factors. Particularly useful factors are GCSF, GMCSF, and MCSF. Any form of such factors known in the art can be used. The form may be an isoform or a differently post-translationally modified form of the factor. The factor may be one which is isolated from humans or other primates or mammals. The factor may be one which is made in a recombinant organism, from bacteria to yeast to sheep. Diseases which are amenable to treatment as described herein include all within the umbrella of inflammatory bowel disease. The phrase "Crohn's Disease not associated with disease X" as used here means that the patient that is being treated according to the method of the invention has not been diagnosed with disease
X. Presently characterized and identifiable specific neutrophil disorders caused by genetic diseases include Chronic Granulomatous Disease, Glycogen storage disease lb, leukocyte adhesion deficiency, Turner's syndrome, Chediak-Higashi, myeloproliferative disease, neutropenias of various causes, and myelodysplastic disease. Ulcerative colitis can be manifested as proctosigmoiditis, left-sided colitis, or pan-colitis. All of these are included in the term "Ulcerative colitis". Pouchitis is an inflammation of an surgically created pouch in the gastrointestinal tract
One goal of treatment is the amelioration, either partial or complete, either temporary or permanent, of patient symptoms, including inflammation of the mucosa, extraintestinal manifestations of the disease, or epithelial damage. Any amelioration is considered successful treatment. This is especially true as amelioration of some magnitude may allow reduction of other medical or surgical treatment which may be more toxic or invasive to the patient. Extraintestinal disease manifestations include those of the liver, bile duct, eyes, and skin. Another goal of the treatment, is to maintain a lack of excess intestinal inflammation in persons who have already achieved remission.
The present invention is based on the theory that the fundamental immune dysregulation of Crohn's Disease results not from an excessive immune response but from a primary immune deficiency. This deficiency likely results from genetic variations in neutrophil and/or macrophage phenotypes interacting with a discrete set of bacteria which suppress neutrophil/macrophage function. In turn, this deficiency provokes a broader compensatory response, amplifymg.inftarmriation, activating- lymphocytes, culminating in Crohn's Disease.
The recent account of patients experiencing prolonged remission after allogeneic bone marrow transplant (BMT) suggests that the marrow phenotype may be central to the pathogenesis of Crohn's Disease. Five patients with Crohn's Disease and chronic myelogenσus leukemia underwent BMT with recurrence of Crohn's Disease in only one patient who remained chimeric with native and transplanted marrow. Conversely, the recurrence of Crohn's Disease in transplanted small bowel reinforces the idea that a critical factor in the development of Crohn's
Disease may be extraintestinal, perhaps partly an intrinsic marrow defect and that Crohn's Disease is not an inherent intestinal abnormality. Consequently, Crohn's
Disease ought to be considered not as a disease of primary intestinal dysfunction but the result of an interaction between marrow constituents and the intestinal environment.
Several genetic syndromes with which Crohn's Disease has been associated provide insight into the possible marrow defects of Crohn's Disease. In particular, five genetic diseases can present with a clinical/histopathological process indistinguishable from Crohn's Disease: chronic granulomatous disease, glycogen storage disease lb, Leukocyte Adhesion Deficiency, Chediak-Higashi syndrome and Turner's syndrome. A distinct deficiency in neutrophil function has been described in each of these syndromes. In addition, Crohn's Disease has been described in association with congenital, autoimmune, and cyclic neutropenias, familial Mediterranean fever, myelodysplastic, and myeloproliferative diseases. The diagnosis of leukemia preceded Crohn's Disease in nearly half the cases in the largest series and diagnosis of myelodysplasia and Crohn's Disease was made concomitantly in half the subjects in another study suggesting a possible causal relationship for the development of Crohn's Disease initiated by an accumulation of dysfunctional neutrophils. These syndromes provide evidence that a variety of functional neutrophil deficiencies can result in a pathophysiology indistinguishable from Crohn's Disease. Disorders of neutrophil/macrophage function represent a potential starting point for understanding Crohn's Disease.
A role for intestinal flora has been established in Crohn's Disease. Reinforced by other evidence, the iriipo tance of the microflbra has been demonstrated by the provocation of inflammation with the direct introduction of ileostomy output into a defunctionalized intestinal segment while a sterile, filtered fraction fails to induce disease activity. While this response is considered non-specific to ubiquitous bacteria, specific alterations in the fecal flora have been identified in patients with Crohn's Disease compared to healthy controls. In Crohn's Disease, Bacteroides tend to be present in increased amounts while Lactobacillus and Bifidobacteria are (hminished, though results of studies are not unanimous. Some Bacteroides species have been shown to impair phagocytosis as well as the microbicidal activity of neutrophils for aerobic bacteria. A discrete subset of the intestinal flora may be responsible for influencing neutrophil/macrophage function
in Crohn's Disease. Recent work with animal models of IBD also support a protective role for Lactobacillus and Bifidobacteria which may act to counter effects of other bacteria and stimulate immune function. The ratio between these different classes of bacteria may be the critical factor in maintaining intestinal health in a susceptible subgroup. Consequently, as suggested by periodontitis, it is unlikely that a single bacteria would be demonstrated as the sole causative agent in Crohn's Disease; instead, these data highlight the complexity of bacterial-immune interactions. The localized nature of the interaction between specific bacteria and leukocytes would account for the specific intestinal manifestations, rather than more systemic findings.
Any explanation of the Crohn's Disease must account for the disease as a twentieth century phenomena in industrialized countries. The disease appears at best rare before Crohn's defining publication in 1932. One possible, relevant, radical change in the past half century may be a shift in intestinal flora. Twentieth century innovations in food preservation with the introduction of refrigeration and other techniques may have produced a fundamental change in the type and amount of bacteria ingested, and may alter the intestinal bacterial content. In comparison to intestinal flora in rural Africa where Crohn's Disease remains rare, the gut flora of westernized countries contain higher concentrations of Bacteroides as well as decreased amounts of Bifidobacteria, perhaps predisposing the intestinal environment to impair the host immune response and set the stage, in the susceptible host, for the development of Crohn's Disease, .
While the human intestinal bacterial flora resists alteration, it can be manipulated. Once established in infancy, the bacterial flora undergoes some changes after weaning but remains remarkably constant throughout life. The protective role of breast feeding against the development of Crohn's Disease may be through promoting Bifidobacteria in higher concentrations and limiting Bacteroides, an effect which has been well documented. The demonstrated association of increased refined carbohydrate intake with Crohn's Disease may be explained through its influence on gut flora as well.
While a change in flora in the susceptible host may alone be sufficient and an important result of the westernized lifestyles, other factors are likely contributory.
The rise in the latter half of this century of smoking and non-steroidal anti-inflammatory drug (NSAID) use, risk factors for Crohn's Disease, may be in part responsible for the increase in Crohn's Disease, though the nature of their influence on the pathophysiology remains uncertain. While several actions of nicotine have been advanced for its influence on Crohn's Disease, nicotine's primary detrimental effect may be on neutrophil function, an effect which has been repeatedly demonstrated. Likewise, though numerous mechanisms have also been proposed for the deleterious effects of NSAIDs on patients with IBD, NSAIDs impairment of neutrophil function may be central to their impact on Crohn's Disease. These factors, each suppressing immune function, may potentiate the same pathway and promote the development or persistence of Crohn's Disease.
The GM-CSF receptor is composed of two subunits:
1) Hs.182378 colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte- macrophage) CSF2RA (CD116) GM-CSF receptor alpha chain
The primary binding subunit of the GM-CSF receptor.
CD116is.a.Iype.I transmembrane protein with about 400 amino acids. Extracellular, transmembrane and cytoplasmϊc domains consist of 297, 27, and 54 amino acid residues, respectively. There is one unit of class I cytokme receptor motif in the extracellular domain and no intrinsic enzymatic activity in the cytoplasmic domain. A number of isoforms are generated by alternative splicing of several soluble forms. All the isoforms are relatively minor species and their physiological function if any is not known. One is a soluble form without the transmembrane domain and the second form is identical to the original one except that the last 25 amino acids of the original receptor is substituted by a 35 amino acids segment
CDl 16 binds GM-CSF with low affinity and binds it with high affinity when
it is co-expressed with the common beta subunit CDwl31 (the common beta subunit (CDwl31) of the GM-CSF, IL-3, and IL-5 receptors). Expression of this subunit is found in various myeloid cells including macrophages, neutrophils, eosinophils, dendritic cells and their precursors.
Tavernier et al. (1991) demonstrated that the high affinity receptor for interleukin-5 (IL5R; 147851) and the receptor for granulocyte-macrophage CSF (CSF2R; 306250) share a beta chain. The finding provides a molecular basis for the observation that IL5 (147850) and CSF2 (138960) can partially interfere with each other's binding and have highly overlapping biologic activities on eosinophils. Kitamura et al. (1991) demonstrated that the receptor for interleukin-3 (IL3RA;
308385) likewise shares a beta subunit with CSF2R.
2) Hs.265262 colony stimulating factor 2 receptor, beta, low-affinity (granulocyte- macrophage) CSF2RB*
(CDwl31) Alternate names for CD l 31 are common beta subunit INTERLEUKIN 5
RECEPTOR, BETA; IL5RB INTERLEUKIN 3 RECEPTOR, BETA; IL3RB * 1-38981 GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR RECEPTOR, BETA; CSF2RB
CDwl 31 does not bind any cytokme by itself. However, it is a component of the high affinity IL-3, GM-CSF and IL-5 receptors. CDwl 31 is tyrosine phosphorylated upon binding of these cytokines to the high affinity receptors. JAK2 tyrosine kinase is associated with CDwl 31 and tyrosine phosphorylates upon stimulation. Tyrosine phosphorylated CD131 binds various signaling molecules with an SH2 domain. These include She, Grb2, SHP1, SHP2, PI3 kinase and STAT5, making it a key signal transducing molecule of the IL-3, GM-CSF and IL-5 receptors.
The following references are expressly incorporated herein for their teachings regarding the common beta subunit of these receptors:
Dirksen, U.; Nishinakamura, R.; Groneck, P.; Hattenhorst, U.; Nogee, L.; Murray, R.; Burdach, S. : Human pulmonary alveolar proteinosis associated with a defect in GM-CSF/TL-3/TL-5 receptor common beta chain expression. J. Clin. Invest. 100:
2211-2217, 1997.
Jenkins, B. J.; D'Andrea, R.; Gonda, T. J. : Activating point mutations in the common beta subunit of the human GM-CSF, IL-3 and IL-5 receptors suggest the involvement of beta subunit dimerization and celltype-specific molecules in signalling. EMBO J. 14: 4276-4287, 1995.
Kitamura, T.; Sato, N.; Arai, K.; Miyajima, A. : Expression cloning of the human IL-3 receptor cDNA reveals a shared beta subunit for the human IL-3 and GM-CSF receptors.
EXAMPLES
Example 1.
This example shows the treatment of 3 Crohn's disease patients with GM- CSF. All three meet the definition of treatment response (CDAI decrease of greater than 70). Patients 2 and 3 are very early in their treatments and it appears likely that they will go into remission. The protocol employed is described below.
Patient 1
Pretreatment CDAI: 340.4
Week l CDAI: 344.4
Week 2 CDAI: 285.6
Week 3 CDAI: 286.6
Week 5 CDAI: 276.4
Week 6 CDAI: 299.4
Week S CDAI: 209.2
Patient 2
Pretreatment CDAI: 349.6
Week 1 CDAI: 274.0
Week 2 CDAI: 227.0
Week 3 CDAI: 216.6
Patient 3-
Pretreatment CDAI: 410.8
Week 1 CDAI: 299.0
Week 2 CDAI: 231.4
MISSING AT THE TIME OF PUBLICATION
This example shows the protocol for a further study of the method of the present invention using G-CSF
An open-labeled trial of G-CSF for 12 weeks for patients with active CD and/or active fistulae secondary to Crohn's disease is initiated. The trial involves two separate, interrelated protocols. In conjunction with this treatment protocol, functional neutrophil studies are performed.
Treatment of active mucosal inflammation in Crohn's disease is the primary focus of Part A. Disease activity is defined by a standard Crohn's disease activity index. Patients are enrolled if they have moderate to severe disease activity using entry criteria outlined below. The study is an open-labeled study of fifteen patients with a dose titration protocol designed to provide neutrophil functional stimulation and achieve a moderate leukocytosis. Each subject is studied for twelve weeks of every day subcutaneous administration of G-CSF. Disease activity is followed by standard CDAI, and the quality of life (IBDQ) scale. We are treating new patients (those not included in our initial study) and offer retreatment to any of the previously treated mucosa patients meeting inclusion criteria.
The primary focus of Part B is to determine the efficacy of G-CSF in patients with perianal and rectovaginal fistula associated with Crohn's disease. Perianal fistulae are common lesions in Crohn's disease and pose a serious threat to the integrity of the normal rectal sphincter. Current therapy for these lesi«ns.are, suboptimal. This study is designed as an open-labeled study of fifteen patients with a dose titration protocol designed to stimulate neutrophil function and associated leukocytosis. Fistulae are assessed by photography, standard examination scoring criteria, as well as a patient subjective assessment of pain, drainage, and overall well-being. Each subject is studied for twelve weeks on daily subcutaneous G-CSF.
Disease activity is also monitored by standard CDAI, and the quality of life QBDQ) scale.
On the basis of extensive investigational and ongoing international clinical experience with G-CSF, the safety profile is well known. In addition, several patients with active Crohn's disease or Crohn's like intestinal disease have been
reported in the medical literature who have been successfully treated (for coexisting disorders) with GM-CSF or G-CSF without any serious or significant adverse effects. Based on the extensive clinical experience with G-CSF and the preliminary evidence of the reported cases, no predictable risks are anticipated at the doses studied. Nevertheless, as this is a new and counterintuitive approach to a chronic inflammatory disease, we are closely monitoring patients for safety and any adverse effects during this protocol.
Treatment Groups
Part A. Treatment of moderate to severe Crohn's disease. Patients are assigned to receive a starting dose of 3.5 micrograms logram/day of G-CSF as a subcutaneous bolus injection. Dosages are adjusted as outlined below, "ANC response-based dosing of G-CSF." Treatment continues for 12 weeks. CDAI is calculated at enrollment, at the start of therapy, and every other week. The Inflammatory Bowel Disease Quality of Life Survey is completed initially and at weeks four, eight, twelve, and sixteen. Follow-up is performed at week 16 with telephone follow-up monthly out to six months from the date of enrollment or up to relapse.
Part B. Treatment of perianal and iectovaginal fistula in Crohn's disease. Patients are assigned to receive a starting dose of 3.5 micrograms kilogram/day of G-CSF as a subcutaneous bolus injection. Dosages are adjusted as outlined below -
"ANC response-based dosing of G-CSF.". Patients who have closed all fistulae before week 8, have G-CSF therapy discontinued at 8 weeks. Patients who have had partial or no response are treated for a full twelve weeks. Fistulae are assessed at weeks 0, 1, 2, 4, 6, 8 and 12 by physical examination scoring criteria, photography, and a subjective patient survey. CDAI is calculated at enrollment, at the start of therapy, and every other week. The Inflammatory Bowel Disease Quality of Life Survey is completed initially and at weeks four, eight, twelve, and sixteen. Follow-up is performed at week 16 with telephone follow-up monthly out to six months from the date of enrollment or up to relapse. Inclusion Criteria
1) Age greater than or equal to 18 years old.
2) History of Crohn's disease for at least three months with extent of disease described endoscopically or radiologically within the past three years. Patients in Part B must have had previous characterization of their perianal disease (e.g., clinical exam, exam under anesthesia (EUA), magnetic resonance imaging, or CT scan).
3) Part A: Crohn's Disease Activity Index (CDAI) > 200 and < 450. Part B: At least 1 draining perianal and/or rectovaginal fistula, refractory to general medical management. Previously treated patients who have had a previous response or remission are eligible for retreatment if they have evidence of increased disease activity manifest by reopening of previously closed fistulas (Part B) or who have a
CDAI of >220 and have an absolute increase of more than 70 from the treatment nadir (Part A).
4) If on aminosalicylate medication (Sulfasalazine, Pentasa, Asacol, Dipentum, Rowasa enemas), must be on for eight weeks and a stable dose for four weeks. If recently discontinued, must be off for at least two weeks prior to study enrollment.
5) If using oral corticosteroids, patients must have been using them for more than 8 weeks, and been on a stable dose (< 20 mg day of prednisone equivalent) for at least 4 weeks prior to trial enrollment. 6) Patients must be off antibiotics for at least two weeks, or if on antibiotics, therapy must be for at least 8 weeks and the patient must be on a stable dose for 4 weeks. Antibiotic use at any time for reasons other than Crohn's disease (e.g. urinary tract infection) is allowed.
7) If female and pre-menopausal, a negative serum beta-HCG must be obtained at the screening visit and use of one of the following forms of contraception must be documented: diaphragm, condom, cervical cap, abstinence or surgical tubal ligation. Patients must agree to use adequate birth control methods until at least 2 months after the last dose of G-CSF.
8) Negative stool tests for routine culture and sensitivity, ova and parasites, and C. difficile toxin.
9) Written informed consent has been obtained and patients must be able to
adhere to the study visit schedule and protocol.
Exclusion Criteria
1) NSATD or ASA ingestion within two weeks of study entry.
2) GI surgery within three months of entry into study. 3) Use of azathioprine, 6-MP, methotrexate or any other immune suppressant in the previous four weeks. Use of infliximab (Remicade) within the previous 12 weeks. Use of any investigation agent (aside from G-CSF) within the previous four weeks or five half-lives of the study medication, whichever is longer.
4) Presence of an ostomy in the mucosa arm. Ostomies are allowed in the fistula arm — a modified CDAI is applied.
5) A patient with any of the following medical conditions: Liver disease with a prothrombin time > 2 second prolongation, portal hypertension, severe hypertension (systolic blood pressure >200 mmHg or a diastolic blood pressure >105 mmHg), renal failure requiring dialysis or a creatinine >2.5, presence of a transplanted organ. 6) Patients with a known, clinically significant, small intestine or colonic stenosis or stricture.
7) A history of leukemia or lymphoma, or other lvmphoproliferative disease, or signs and symptoms of lvmphoproliferative disease such as abnormal cells on CBC, or suggestive physical exam findings of lymphadenopathy of unusual size or location.
8) Evidence of abscess or active Crohn's disease related infections in need of surgical drainage
9) Known recent substance abuse (drugs or alcohol).
10) History of gout 11) Significant unexplained abnormalities in any of the prescreening blood work.
12) Known hypersensitivity to E. coli-derived proteins.
Physician Visits
New patients are screened within 14 days of initiating the medication with a history, physical and recall estimation of a Crohn's disease activity index (CDAI). If the estimated CDAI is > 200 and < 450, subjects have a CBC drawn, beta-HCG (if female and premenopausal) and complete a diary for 1 consecutive week within the next 14 days. The subject will return for initiation at day 0. If subject meets entry/exclusion criteria, blood is drawn for CBC, electrolytes, BUN, creatinine, liver enzymes (AST, ALT, alkaline phosphatase, bilirubin), albumin, ESR, and CRP. Instruction is provided for self-administration of the medication (subcutaneous injection). Subjects are titrated to a stable WBC response . Patients are seen and examined by a physician on weeks 0, 1, 2, 4, 6, 8, 10, 12, and for the required post treatment visit at week 16. A daily diary is completed throughout the period of administration of the medication. Telephone calls are performed monthly, starting at the conclusion of G-CSF therapy and continued through six months to assess disease activity and adverse effects. At each visit, patients are provided a sufficient supply of medication to continue administration through the next visit.
Laboratory Blood Tests
CBC are performed at screening, at the times indicated by the flow sheet during treatment, and at the scheduled visit 4 weeks after completion of therapy. A panel of serum chemistry studies (electrolytes, BUN, creatinine, liver enzymes
(AST, ALT, alkaline phosphatase, bilirubin) is measured at screening, and at weeks 4, 8, 12, and 16. C-Reactive Protein (CRP) and erythrocyte sedimentation rate (ESR) are measured at week 0, 2, 4, 8, 12, and 16. Laboratory results that deviate significantly from baseline (except the WBC and alkaline phosphatase) are repeated by obtaining new samples.
ANC response-based dosing of G-CSF
Initial dose: 3.5 micrograms per kilogram per day SQ. Doses are based on the patient enrollment weight.
Decreases based on HIGH ANC: Patients with an ANC>60,000/mm3 (60.0 x 109 L) have their dose decreased by 1.0 micrograms per kilogram per day. Otherwise, subsequent dose adjustments
are made in 0.5 mcg/kg/day increments. CBC with differential are drawn in one week. Subsequent dose modifications are made as follows.
1. For a patient with an ANC >50,000/mm3, the next scheduled dose is lowered by 0.5 mcg/kg/day. The ANC is rechecked in 1 week. 2. For a patient with an ANC of between 40-50,000/ mm3, the next scheduled dose is kept the same. The ANC is rechecked in 1 week.
3. For a patient with two consecutive ANC measurements between 40-50,000/ mm3, the next ANC measurement is performed at the next scheduled physician visit.
Increases based on LOW ANC Patients with an ANC<30,000/mm3 (30.0 x 109/L) have their dose increased by 1.0 micrograms per kilogram per day. CBC with differential are drawn in one week. Subsequent dose modifications are made as follows.
1. For a patient with an ANC <40,000/mm3, the next scheduled dose is increased by 0.5 mcg/kg day. The ANC is rechecked in 1 week. 2. For a patient with an ANC of between 40-50,000/ mm3, the next scheduled dose is kept the same. The ANC is rechecked in 1 week.
3. For a patient with two consecutive ANC measurements between 40-50,000/ mm3, the next ANC measurement is performed at the next scheduled physician visit.
Physicians may decrease dosages as necessary due to severe bone pain. Once the ANC has been in the target range (40-50) on two consecutive measurements, monitoring is increased to the next scheduled physician visit. Physician or nurse coordinators review the CBC results and call the patient with any dosage change.
Example 4
This example shows the protocol for a further study of the method of the present invention using GM-CSF.
An open-labeled, pilot, dose escalation study of GM-CSF is performed in patients with CD. Fifteen patients are enrolled with active disease (Crohn's Disease
Activity Index- CDAI >200 and <450). Patients are required not to have used steroids, azathioprine of other immune modulator for four weeks. NSAID use is not allowed for two weeks prior to or throughout the study. An open-labeled dose escalation study is conducted in three groups of five (4 mcg/kg/day ; 6 mcg/kg/day; 8mcg/kg/day) Neutrophil function is tested before the initiation of GM-CSF and at week eight (two weeks after the completion of six weeks of administration of the medication) to assess neutrophil chemotaxis and superoxide production. CBC is examined on day 0, 7 and then weekly except for week 5. C-Reactive Protein (CRP) and erythrocyte sedimentation rate (ESR) is examined every two weeks. Patients are examined weekly during the trial with telephone calls once a week as well for safety monitoring. Patients complete a daily diary throughout the study. CDAI is calculated weekly and an Inflammatory Bowel Disease Quality of Life Survey is completed at week 0, week four and week eight. Follow-up is performed at week 8, and 12 with telephone follow-up monthly for six months.
Inclusion criteria:
1) Age over 16 years old.
2) History of Crohn's disease for at least three months with extent of disease described endoscopically or radiologically within the past two years.
3) Crohn's disease activity index > 200 and < 450. 4) If on a mesalamine medication (sulfasalazine, Pentasa, Asacol. Dipentϋm,
Rowasa enemas), must be on for eight weeks and a stable dose for four weeks.
5) If on antibiotics, must be on stable doses for at least six weeks.
6) If female and pre-menopausal, a negative serum beta-HCG must be obtained at the screening visit and use of one of the following forms of contraception must be documented: diaphragm, condom, cervical cap, abstinence or surgical tubal ligation.
7) Negative stool tests for routine culture and sensitivity and C. difficile assay.
8) Written informed consent has been obtained.
Exclusion criteria:
1) NSAID or ASA ingestion within two weeks of study entry.
2) GI surgery within three months of entry into study.
3) Use of steroids, azathioprine, 6-MP, methotrexate or any other immune suppressant in the previous four weeks. Use of infliximab (Remicade) within the previous 12 weeks. Use of any investigation agent within the previous four weeks or five half-lives of the study medication, whichever is longer.
4) Presence of an ostomy.
5) A patient with any of the following medical conditions: Liver disease with a prothrombin time > 2 second prolongation; portal hypertension ; severe hypertension
(systolic blood pressure >200 mmHg or a diastolic blood pressure >105 mmHg); renal failure requiring dialysis or a creatinine >2.5.
6) Patients with a clinically significant tight small intestine or colonic stenosis or stricture. 7) A history of leukemia or lymphoma
Physician Visits
Subjects are screened within 14 days of initiating the medication with a history, physical and recall estimation of a Crohn's disease activity index (CDAI). If the estimated CDAI is > 200 and < 450, subjects have a CBC drawn, beta-HCG if female and premenopausal) and complete a diary for 1 consecutive week within the next 14 days. The subject returns for initiation at day 0. If subject meets entry/exclusion criteria, blood is drawn for CBC, BUN, creatinine, liver enzymes (AST, ALT, alkaline phosphatase, bilirubin), Albumin, ESR, CRP and neutrophil studies (see section below). Instruction is provided for self-administration of the medication (IM injection). Subject visits weekly except for week five. Visits are required at week 8 and 12. A daily diary is completed throughout the period of administration of the medication. Telephone calls are performed weekly during the medication period through week 8 and monthly through six months to assess disease activity and adverse effects. At each visit, patients are provided a sufficient supply
of medication in preloaded syringes to continue administration through the next physician visit.
Dose adjustment
Patients with an absolute neutrophil count of > 30,000 have dose held for 3 days when a CBC rechecked. If the ANC is < 30,000 the dose is resumed at 2 mcg kg/day lower than the dose administered when the leukocytosis occurred.
Study Failure
Patients are considered to have failed therapy if their CDAI increases by >125 points on two separate occasions during the study or deteriorates in any way considered significantly worse by the physicians.
Assessment of Treatment Efficacy
Assessment is determined by a decrease in CDAI with remission considered a CDAI < 150 and in significant improvement considered a decrease of at least 70 points. Treatment failure is considered inability to tolerate the therapy, a worsening of disease (more than 100 points) or need to use additional medication for management of Crohn's disease. IBDQ is also performed at 3 time points: at initiation of therapy at week 3 and at week 6. Note: CDAI is calculated with and without febrile episodes. As GM-CSF can induce febrile episodes, the CDAI, which includes fevers as one extra intestinal manifestation in the calculation of disease activity score, for calculating the index, is calculated with and without fevers assessed. Entry criteria are calculated including febrile episodes.
Possible Adverse Events
The principal concern is lack of effect or an exacerbation of disease. The serious toxicities reported have been associated with doses greater than 10 mcg/kg/day. Systemic symptoms were identified in 27% of patients in a review of
295 patients in phase I and phase II studies. Bone pain (21%) and fever (18%) were the most commonly reported events though these symptoms were severe in <2% of patients. Fevers can be well-managed with acetaminophen. These reactions are less common at doses proposed in this protocol. Skin reactions at sites of subcutaneous
injection are usually mild or moderate and resolve on discontinuation. The range of doses to be tested here is 4-8 mcg/kg/day.
Patients receiving GM-CSF (Sargramostim) have experienced fever; chills; nausea; vomiting; diarrhea; fatigue; weakness; headache; decreased appetite; thrombosis; rapid or irregular heartbeat or other heart problems; feeling of faintness; facial flushing; pain in the bones, muscles, chest abdomen, or joints; local reaction at the site of injection; rashes; and kidney and liver dysfunction.
There have been infrequent reports of fluid accumulation or worsening of pre-existing fluid accumulation in the extremities, in the lungs, and around the heart which may result in breathing problems or heart failure. Rarely, patients have developed acute allergic reactions. There have been reports of low blood pressure, hypoxia (low oxygen), transient loss of consciousness, and difficulty in breathing after the first injection of Sargramostim. These signs may or may not recur with additional injections of Sargramostim. Patients with prior heart, lung, kidney or liver problems may have worsening of their symptoms following administration of
Sargramostim. There may be other side effects that could occur.
Claims (59)
1. A method of treating Crohn's Disease comprising: administering to a patient with Crohn's Disease not associated with Glycogen Storage Disease lb an immune stimulatory amount of an agonist of CD 114 (Granulocyte Colony Stimulating Factor Receptor (G-CSFR)).
2. A method of treating Crohn's Disease comprising: administering to a patient with Crohn's Disease not associated with Glycogen Storage Disease lb an immune stimulatory amount of an agonist of CDl 16 (Granulocyte-Macrophage Colony Stimulating Factor Receptor (GM- CSFR)).
3. A method of treating Crohn' s Disease comprising: administering to a patient with Crohn's Disease not associated with Chronic Granulomatous Disease an immune stimulatory amount of an agonist of CDl 14 (Granulocyte Colony Stimulating Factor Receptor (G-CSFR)).
4. A method of treating Crohn's Disease comprising: administering to a patient with Crohn's Disease not associated with Chronic Granulomatous Disease an immune stimulatory amount of an agonist of CDl 16 (Granulocyte-Macrophage Colony Stimulating Factor Receptor (GM- CSFR)).
5. A method of treating Crohn's Disease comprising: administering to a patient with Crohn's Disease not associated with a presently characterized and identifiable specific neutrophil disorder caused by a genetic disease an immune stimulatory amount of an agonist of CD 114 (Granulocyte Colony Stimulating Factor Receptor (G-CSFR)).
6. A method of treating Crohn's Disease comprising: administering to a patient with Crohn's Disease not associated with a presently characterized and identifiable specific neutrophil disorder caused by a genetic disease an immune stimulatory amount of an agonist of CDl 16 (Granulocyte-Macrophage Colony Stimulating Factor Receptor (GM-CSFR)).
7. A method of treating pouchitis comprising: administering to a patient with pouchitis an immune stimulatory amount of an agonist of CDl 14 (Granulocyte Colony Stimulating Factor Receptor (G-CSFR)).
8. A method of treating pouchitis comprising: administering to a patient with pouchitis an immune stimulatory amount of an agonist of CDl 16 (Granulocyte-Macrophage Colony Stimulating Factor Receptor (GM-CSFR)).
9. A method of preventing or reducing the risk of fistula recurrence, comprising: administering to a patient with Crohn's disease who has previously had a fistula, with an immune stimulatory amount of an agonist of CDl 14 (Granulocyte Colony Stimulating Factor Receptor (G-CSFR)), whereby the risk of recurrence of a fistula is reduced.
10. A method of preventing or reducing the risk of fistula recurrence, comprising: administering to a patient with Crohn's disease who has previously had a fistula, with an immune stimulatory amount of an agonist of CD116 (Granulocyte-Macrophage Colony Stimulating Factor Receptor (GM-CSFR)), whereby the risk of recurrence of a fistula is reduced.
11. The method of claim 1 , 2, 3, 4, 5, or 6 wherein the patient has mucosal inflammatory disease of at least one of the small intestine, colon, or rectum, and the amount of colony stimulating factor administered is sufficient to reduce the mucosal inflammation.
12. The method of claim 11 wherein the amount of colony stimulating factor administered is sufficient to induce remission of the mucosal disease.
13. The method of claim 1 , 2, 3, 4, 5, or 6 wherein the patient has epithelial damage of at least one of the small intestine, colon, or rectum, and the amount of colony stimulating factor administered is sufficient to repair the epithelial damage.
14. The method of claim 1, 2, 3, 4, 5, or 6 wherein the amount of colony stimulating factor administered is sufficient to reduce the patient's symptoms.
15. The method of claim 1, 2, 3, 4, 5, or 6 wherein the patient has a fistula or a perianal abscess, and the amount of colony stimulating factor administered is sufficient to reduce the fistula or perianal abscess.
16. The method of claim 1 , 2, 3, 4, 5, or 6 wherein the patient is in remission.
17. The method of claim 1 , 2, 3, 4, 5, or 6 wherein the patient has received surgical therapy of affected portions of the gastrointestinal tract.
18. The method of claim 1 , 2, 3, 4, 5, or 6 wherein the patient has an extraintestinal manifestation of Crohn's disease and the amount of colony stimulating factor administered is sufficient to reduce the extraintestinal manifestation.
19. The method of claim 18 wherein the extraintestinal manifestation is an inflammatory eye disorder.
20. The method of claim 19 wherein the inflammatory eye disorder is selected from the group consisting of: iritis, uveitis, and episcleritis.
21. The method of claim 19 wherein the extraintestinal manifestation a skin disorder.
22. The method of claim 21 wherein the skin disorder is selected from the group consisting of: pyoderma gangrenosum and erythema nodosum.
23. The method of claim 18 wherein the extraintestinal manifestation is a liver disorder.
24. The method of claim 23 wherein the liver disorder is primary sclerosing cholangitis.
25. The method of claim 18 wherein the extraintestinal manifestation is bile duct disease.
26. The method of any of claims 1 to 10 wherein a reduced dose of an immunosuppressive agent is also administered to the patient.
27. The method of claim 26 wherein the agent is selected from the group consisting of: corticosteroid, 6-mercaptopurine, azathioprine, and methotrexate.
28. The method of claim 18 wherein the extraintestinal manifestation is stomach inflammation.
29. The method of claim 18 wherein the extraintestinal manifestation is esophageal disease.
30. The method of claim 1, 3, 5, 7, or 9 wherein the agonist is G-CSF.
31. The method of claim 2, 4, 6, 8, or 10 wherein the agonist is GM-CSF.
32. The method of claim 1, 3, 5, 7, or 9 wherein the agonist is a peptidomimetic of G-CSF.
33. The method of claim 1 , 3, 5, 7, or 9 wherein the agonist is a non- peptidomimetic of G-CSF.
34. A method of treating Crohn's Disease comprising: administering to a patient with Crohn's Disease not associated with
Glycogen Storage Disease lb an immune stimulatory amount of an agonist of CDwl31 (the common beta subunit of the receptors for GM-CSF, IL-3, and 11-5).
35. A method of treating Crohn's Disease comprising: administering to a patient with Crohn's Disease not associated with Chronic Granulomatous Disease an immune stimulatory amount of an agonist of
CDwl 31 (the common beta subunit of the receptors for GM-CSF, IL-3, and 11-5).
36. A method of treating Crohn's Disease comprising: administering to a patient with Crohn's Disease not associated with a presently characterized and identifiable specific neutrophil disorder caused by a genetic disease an immune stimulatory amount of an agonist of Cdwl 31 (the common beta subunit of the receptors for GM-CSF, IL-3, and U-5).
37. A method of treating pouchitis comprising: administering to a patient with pouchitis an immune stimulatory amount of an agonist of CDwl 31 (the common beta subunit of the receptors for GM-CSF, IL-3, and 11-5).
38. A method of preventing or reducing the risk of fistula recurrence, comprising: administering to a patient with Crohn's disease who has previously had a fistula, with an immune stimulatory amount of an agonist of CDwl 31 (the common beta subunit of the receptors for GM-CSF, IL-3, and 11-5), whereby the risk of recurrence of a fistula is reduced.
39. The method of any of claims 34 -38 wherein the agonist is IL-3.
40. The method of any of claims 34 -38 wherein the agonist is IL-5.
41. The method of any of claims 34-36 wherein the patient has mucosal inflammatory disease of at least one of the small intestine, cplon, or rectum, and the amount of colony stimulating factor administered is sufficient to reduce the mucosal inflammation.
42. The method of claim 41wherein the amount of colony stimulating factor administered is sufficient to induce remission of the mucosal disease.
43. The method of any of claims 34-36 wherein the patient has epithelial damage of at least one of the small intestine, colon, or rectum, and the amount of colony stimulating factor administered is sufficient to repair the epithelial damage.
44. The method of any of claims 34-36 wherein the amount of colony stimulating factor administered is sufficient to reduce the patient's symptoms.
45. The method of any of claims 34-36 wherein the patient has a fistula or a perianal abscess, and the amount of colony stimulating factor administered is sufficient to reduce the fistula or perianal abscess.
46. The method of any of claims 34-36 wherein the patient is in remission.
47. The method of any of claims 34-36 wherein the patient has received surgical therapy of affected portions of the gastrointestinal tract
48. The method of any of claims 34-36 wherein the patient has an extraintestinal manifestation of Crohn's disease and the amount of colony stimulating factor administered is sufficient to reduce the extraintestinal manifestation.
49. The method of claim 48 wherein the extraintestinal manifestation is an inflammatory eye disorder.
50. The method of claim 49 wherein the inflammatory eye disorder is selected from the group consisting of: iritis, uveitis, and episcleritis.
51. The method of claim 49 wherein the extraintestinal manifestation a skin disorder.
52. The method of claim 51 wherein the skin disorder is selected from the group consisting of: pyoderma gangrenosum and erythema nodosum.
53. The method of claim 48 wherein the extraintestinal manifestation is a liver disorder.
54. The method of claim 53 wherein the liver disorder is primary scl erasing cholangitis.
55. The method of claim 48 wherein the extraintestinal manifestation is bile duct disease.
56. The method of any of claims '34-38 wherein a reduced dose of an immunosuppressive agent is also administered to the patient.
57. The method of claim 56 wherein the agent is selected from the group consisting of: corticosteroid, 6-mercaptopurine, azathioprine, and methotrexate.
58. The method of claim 48 wherein the extraintestinal manifestation is stomach inflammation.
59. The method of claim 48 wherein the extraintestinal manifestation is esophageal disease.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/637,062 US6500418B1 (en) | 1999-02-12 | 2000-08-11 | Stimulating neutrophil function to treat inflammatory bowel disease |
US09/637,062 | 2000-08-11 | ||
PCT/US2001/024857 WO2002013866A2 (en) | 2000-08-11 | 2001-08-09 | Stimulating neutrophil function to treat inflammatory bowel disease |
Publications (2)
Publication Number | Publication Date |
---|---|
AU2001279235A1 true AU2001279235A1 (en) | 2002-05-23 |
AU2001279235B2 AU2001279235B2 (en) | 2006-07-13 |
Family
ID=24554379
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2001279235A Ceased AU2001279235B2 (en) | 2000-08-11 | 2001-08-09 | Stimulating neutrophil function to treat inflammatory bowel disease |
AU7923501A Pending AU7923501A (en) | 2000-08-11 | 2001-08-09 | Stimulating neutrophil function to treat inflammatory bowel disease |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU7923501A Pending AU7923501A (en) | 2000-08-11 | 2001-08-09 | Stimulating neutrophil function to treat inflammatory bowel disease |
Country Status (8)
Country | Link |
---|---|
US (4) | US6500418B1 (en) |
EP (1) | EP1309339B1 (en) |
AT (1) | ATE374620T1 (en) |
AU (2) | AU2001279235B2 (en) |
CA (1) | CA2419855A1 (en) |
DE (1) | DE60130775T2 (en) |
WO (1) | WO2002013866A2 (en) |
ZA (1) | ZA200301630B (en) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7838539B2 (en) * | 1999-07-28 | 2010-11-23 | The Board Of Trustees Of The Leland Stanford Junior University | Nicotine receptor agonists in stem cell and progenitor cell recruitment |
US20020141970A1 (en) * | 2001-03-05 | 2002-10-03 | Pettit Dean K. | Stable aqueous solutions of granulocyte macrophage colony-stimulating factor |
CA2449987C (en) * | 2001-06-07 | 2015-11-03 | Christine N. Sang | Treatment of central neuropathic pain |
US7129049B2 (en) * | 2003-12-22 | 2006-10-31 | Regents Of The University Of Minnesota | Method of detecting equine glycogen storage disease IV |
WO2008085963A1 (en) * | 2007-01-05 | 2008-07-17 | University Of Florida Research Foundation, Inc. | Materials and methods for the detection, prevention and treatment of autoimmune disease |
BRPI0917130A2 (en) * | 2008-08-25 | 2015-12-01 | Centocor Ortho Biotech Inc | biomarkers for anti-tnf treatment of ulcerative colitis and related disorders |
US10105415B2 (en) | 2014-07-24 | 2018-10-23 | Reponex Pharmaceuticals A/S | Compositions comprising granulocyte-macrophage colony-stimulating factor for the treatment of inflammatory bowel disease |
US20230041197A1 (en) | 2018-06-20 | 2023-02-09 | Progenity, Inc. | Treatment of a disease of the gastrointestinal tract with an immunomodulator |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4961926A (en) | 1987-11-19 | 1990-10-09 | Sloan-Kettering Institute For Cancer Research | Methods for prevention and treatment of mucositis with granulocyte colony stimulating factor |
EP0447523A4 (en) | 1989-10-10 | 1991-11-27 | Amgen Inc. | Compositions and methods for treating or preventing infections in canine and feline animals |
IL96477A0 (en) | 1989-12-01 | 1991-08-16 | Amgen Inc | Megakaryocyte production |
WO1992016192A1 (en) | 1991-03-15 | 1992-10-01 | Amgen Inc. | Pulmonary administration of granulocyte colony stimulating factor |
US5581476A (en) | 1993-01-28 | 1996-12-03 | Amgen Inc. | Computer-based methods and articles of manufacture for preparing G-CSF analogs |
US5654186A (en) | 1993-02-26 | 1997-08-05 | The Picower Institute For Medical Research | Blood-borne mesenchymal cells |
JP3441763B2 (en) | 1993-06-30 | 2003-09-02 | キリン−アムジエン・インコーポレーテツド | Monoclonal antibody and method for measuring human G-CSF using the monoclonal antibody |
WO1995009622A1 (en) | 1993-10-06 | 1995-04-13 | Peptide Technology Limited | Polyunsaturated fatty acids and uses thereof |
US6288030B1 (en) | 1993-12-22 | 2001-09-11 | Amgen Inc. | Stem cell factor formulations and methods |
US6037324A (en) | 1996-01-04 | 2000-03-14 | Leukosite, Inc. | Inhibitors of MAdCAM-1-mediated interactions and methods of use therefor |
US5814308A (en) | 1996-03-26 | 1998-09-29 | Zhang; Ke | Methods for the treatment of gastrointestinal tract disorders |
DE19835080A1 (en) * | 1998-07-27 | 2000-02-03 | Doecke Wolf Dietrich | Agent for the treatment of Crohn's disease |
WO2000047195A1 (en) * | 1999-02-12 | 2000-08-17 | Washington University | Stimulating neutrophil function to treat inflammatory bowel disease |
DK1175511T3 (en) * | 1999-04-13 | 2006-01-09 | Agres Ltd | Methods and test kits for collecting and storing biological samples |
-
2000
- 2000-08-11 US US09/637,062 patent/US6500418B1/en not_active Expired - Lifetime
-
2001
- 2001-08-09 AT AT01957497T patent/ATE374620T1/en not_active IP Right Cessation
- 2001-08-09 DE DE60130775T patent/DE60130775T2/en not_active Expired - Lifetime
- 2001-08-09 EP EP01957497A patent/EP1309339B1/en not_active Expired - Lifetime
- 2001-08-09 AU AU2001279235A patent/AU2001279235B2/en not_active Ceased
- 2001-08-09 CA CA002419855A patent/CA2419855A1/en not_active Abandoned
- 2001-08-09 AU AU7923501A patent/AU7923501A/en active Pending
- 2001-08-09 WO PCT/US2001/024857 patent/WO2002013866A2/en active IP Right Grant
-
2002
- 2002-10-01 US US10/260,354 patent/US7060262B2/en not_active Expired - Fee Related
-
2003
- 2003-02-27 ZA ZA200301630A patent/ZA200301630B/en unknown
-
2005
- 2005-04-06 US US11/099,461 patent/US7407931B2/en not_active Expired - Fee Related
-
2006
- 2006-04-07 US US11/399,456 patent/US7641894B2/en not_active Expired - Fee Related
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US7641894B2 (en) | Stimulating neutrophil function to treat inflammatory bowel disease | |
Butterfield et al. | Interferon-α treatment of six patients with the idiopathic hypereosinophilic syndrome | |
AU2001279235A1 (en) | Stimulating neutrophil function to treat inflammatory bowel disease | |
Wilde et al. | Oprelvekin: a review of its pharmacology and therapeutic potential in chemotherapy-induced thrombocytopenia | |
Sedano et al. | Tofacitinib for the treatment of three immune-mediated conditions in one patient: ulcerative colitis, pyoderma gangrenosum, and alopecia areata | |
AU764681B2 (en) | Stimulating neutrophil function to treat inflammatory bowel disease | |
JP5416589B2 (en) | G-CSF containing agent for preventing and treating diabetic peripheral neuropathy | |
Belhadjali et al. | Sweet's syndrome associated with multiple myeloma | |
Brundage et al. | Interleukin-6 infusion blunts proinflammatory cytokine production without causing systematic toxicity in a swine model of uncontrolled hemorrhagic shock | |
US20070141053A1 (en) | Treatment of inflammatory respiratory diseases | |
Baños et al. | Effectiveness of immunomodulating treatment (thymostimulin) in chronic obstructive pulmonary disease | |
JP2002536404A5 (en) | ||
WO2005049068A1 (en) | Use of interferon tau for the treatment of obesity and for promotion of weight loss | |
EP4126015A1 (en) | Method for preventing or treating lung infection and lung inflammation | |
Mahvi et al. | CHROIVC DISSEMINIATED HISTOPLASMOSIS II {AN APPA RENTLY IMMUI\O-C OMPETEI\Tb EL GIAI { | |
Sacca et al. | Russo A, Villiva M, Notturni A, Fontana V, Borgognoni I | |
Boland et al. | Selected Abstracts of the Belgian Society of Internal Medicine | |
UA46731C2 (en) | METHOD OF TREATMENT OF PERSONS SUFFERING FROM SEPSIS |