AU2001274084A1 - The non-mevalonate isoprenoid pathway - Google Patents

The non-mevalonate isoprenoid pathway

Info

Publication number
AU2001274084A1
AU2001274084A1 AU2001274084A AU7408401A AU2001274084A1 AU 2001274084 A1 AU2001274084 A1 AU 2001274084A1 AU 2001274084 A AU2001274084 A AU 2001274084A AU 7408401 A AU7408401 A AU 7408401A AU 2001274084 A1 AU2001274084 A1 AU 2001274084A1
Authority
AU
Australia
Prior art keywords
variants
biosynthesis
isoprenoids
inhibitors
encoding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001274084A
Inventor
Petra Adam
Adelbert Bacher
Wolfgang Eisenreich
Monika Fellermeier
Stefan Hecht
Felix Rohdich
Christoph A. Schuhr
Juraithip Wungsintaweekul
Meinhart H. Zenk
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of AU2001274084A1 publication Critical patent/AU2001274084A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Intermediate (A), optionally isotopically labeled, in the mevalonate-independent pathway of isoprenoid biosynthesis, is new. Independent claims are also included for the following: (a) aqueous cell fluid containing (I) and (A), with at least one present at higher concentration than in fluids from normal cells or organisms; (b) preparing (A); (c) enzymes (B) that convert (I) to (A); (d) isolated, purified nucleic acid (C) encoding (B); (e) DNA vectors containing (C); (f) recombinant cells containing the vector of (e); (g) screening chemical libraries for inhibitors of isoprenoid biosynthesis based on blocking conversion of (I); (h) identifying inhibitor-resistant variants of (B); (i) (B) variants identified by method (h); (j) nucleic acid (C') encoding the variants of (f); (k) (l) vectors containing (C'); (l) cells containing the vectors of (l); (m) imparting herbicide resistance to plants by incorporation of (C'); (n) weed control that involves growing crop plants of (n); (o) inhibitors (D) identified by method (g); (p) composition for inhibiting biosynthesis of isoprenoids containing (D); and (q) inhibiting biosynthesis of isoprenoids by treatment with (D) or the compositions of (q).
AU2001274084A 2000-06-05 2001-06-01 The non-mevalonate isoprenoid pathway Abandoned AU2001274084A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10027821A DE10027821A1 (en) 2000-06-05 2000-06-05 New intermediate in isoprenoid biosynthesis, useful in screening for potential herbicides, comprises mutant encoding-enzymes sequences for imparting herbicide resistance
DE10027821 2000-06-05
PCT/EP2001/006255 WO2001094561A2 (en) 2000-06-05 2001-06-01 The non-mevalonate isoprenoid pathway

Publications (1)

Publication Number Publication Date
AU2001274084A1 true AU2001274084A1 (en) 2001-12-17

Family

ID=7644765

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001274084A Abandoned AU2001274084A1 (en) 2000-06-05 2001-06-01 The non-mevalonate isoprenoid pathway

Country Status (6)

Country Link
US (2) US7288367B2 (en)
EP (1) EP1287145B1 (en)
AT (1) ATE415479T1 (en)
AU (1) AU2001274084A1 (en)
DE (2) DE10027821A1 (en)
WO (1) WO2001094561A2 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE373715T1 (en) 1999-08-04 2007-10-15 Adelbert Bacher ISOPRENOID BIOSYNTHESIS
DE10027821A1 (en) 2000-06-05 2001-12-06 Adelbert Bacher New intermediate in isoprenoid biosynthesis, useful in screening for potential herbicides, comprises mutant encoding-enzymes sequences for imparting herbicide resistance
US6660507B2 (en) 2000-09-01 2003-12-09 E. I. Du Pont De Nemours And Company Genes involved in isoprenoid compound production
DE10201458A1 (en) * 2001-04-11 2002-10-17 Adelbert Bacher New proteins involved in isoprenoid biosynthesis, useful in screening for inhibitors, also new intermediates, potential therapeutic agents, nucleic acids and antibodies
WO2002092800A2 (en) * 2001-05-15 2002-11-21 Max-Planck-Gesellschaft Crystal structure of 2c-methyl-d-erythritol 2,4-cyclodiphosphate synthase
JP4607451B2 (en) 2001-07-20 2011-01-05 バイオエージェンシー・アーゲー Organophosphorus compounds for activating gamma / delta T cells
BR122021005287B1 (en) 2010-06-02 2022-02-22 Evolva, Inc Recombinant host comprising a recombinant gene for producing steviol or steviol glycoside
WO2013022989A2 (en) 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
WO2014122227A2 (en) 2013-02-06 2014-08-14 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
MY184253A (en) 2013-02-11 2021-03-29 Evolva Sa Efficient production of steviol glycosides in recombinant hosts
WO2016023844A1 (en) 2014-08-11 2016-02-18 Evolva Sa Production of steviol glycosides in recombinant hosts
SG10201901957SA (en) 2014-09-09 2019-04-29 Evolva Sa Production of steviol glycosides in recombinant hosts
CA2973674A1 (en) 2015-01-30 2016-08-04 Evolva Sa Production of steviol glycosides in recombinant hosts
US10604743B2 (en) 2015-03-16 2020-03-31 Dsm Ip Assets B.V. UDP-glycosyltransferases
JP2018522569A (en) 2015-08-07 2018-08-16 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． Production of steviol glycosides in recombinant hosts
BR112018071144B1 (en) 2016-04-13 2023-04-18 Evolva Sa RECOMBINANT HOST CELL PRODUCING ONE OR MORE STEVIOL GLYCOSIDES OR STEVIOL GLYCOSIDE COMPOSITION IN A CELL CULTURE AND METHODS FOR PRODUCING ONE OR MORE STEVIOL GLYCOSIDES OR STEVIOL GLYCOSIDE COMPOSITION, AS WELL AS CULTURE CULTURE AND CELL LYSATE FROM SAID RECOMBINANT HOST CELL
US10815514B2 (en) 2016-05-16 2020-10-27 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0154204B1 (en) * 1984-03-06 1994-01-12 Mgi Pharma, Inc. Herbicide resistance in plants
GB9315751D0 (en) 1993-07-30 1993-09-15 Zeneca Ltd Dna,dna constructs,cells and plants derived therefrom
US5885782A (en) 1994-09-13 1999-03-23 Nce Pharmaceuticals, Inc. Synthetic antibiotics
US6107071A (en) 1996-09-24 2000-08-22 Smithkline Beecham Corporation Histidinol dehydrogenase
US5858367A (en) * 1997-03-27 1999-01-12 Case Western Reserve University Methods for screening for antimicrobials utilizing AarC and compositions thereof
AU746684B2 (en) 1997-09-02 2002-05-02 Washington State University Research Foundation Nucleic and amino acid sequences for a novel transketolase from $i (Mentha Piperita)
DE19752700A1 (en) * 1997-11-28 1999-06-02 Hoechst Schering Agrevo Gmbh 1-Deoxy-D-xylulose-5-phosphate synthase protein and modulators
WO1999052938A2 (en) * 1998-04-14 1999-10-21 Jomaa Hassan Identification of chemical active agents for inhibiting the 1-desoxy-d-xylulose-5-phosphate biosynthetic pathway in parasites
EP1078045A1 (en) 1998-05-13 2001-02-28 University Of Maryland College Park Methods of modifying the production of isopentenyl pyrophosphate, dimethylallyl pyrophosphate and/or isoprenoids
IL141888A0 (en) * 1998-09-22 2002-03-10 Jomaa Pharmaka Gmbh Genes of the 1-deoxy-d-xylulose biosynthetic pathway
WO2000036138A1 (en) * 1998-12-14 2000-06-22 Adelbert Bacher Screening for inhibitors of terpenoids biosynthesis
CA2374608A1 (en) * 1999-05-21 2000-11-30 Jomaa Pharmaka Gmbh Use of genes of the deoxy-d-xylulose phosphate biosynthetic pathway for altering the concentration of isoprenoid
ATE373715T1 (en) 1999-08-04 2007-10-15 Adelbert Bacher ISOPRENOID BIOSYNTHESIS
DE10021688A1 (en) * 2000-05-05 2001-11-15 Hassan Jomaa New DNA sequences involved in isoprenoid biosynthesis, useful in screening for compounds with e.g. antimicrobial and herbicidal activity
DE10027821A1 (en) 2000-06-05 2001-12-06 Adelbert Bacher New intermediate in isoprenoid biosynthesis, useful in screening for potential herbicides, comprises mutant encoding-enzymes sequences for imparting herbicide resistance
DE60140312D1 (en) 2000-08-07 2009-12-10 Monsanto Technology Llc Am methyl-d-erythriol phosphat reaktionsweg beteiligte gene
DE10201458A1 (en) 2001-04-11 2002-10-17 Adelbert Bacher New proteins involved in isoprenoid biosynthesis, useful in screening for inhibitors, also new intermediates, potential therapeutic agents, nucleic acids and antibodies
WO2002092800A2 (en) 2001-05-15 2002-11-21 Max-Planck-Gesellschaft Crystal structure of 2c-methyl-d-erythritol 2,4-cyclodiphosphate synthase
AU2002322265A1 (en) 2001-06-18 2003-01-02 Structural Genomix Crystals and structure of 2c-methyl-d-erythritol 2,4-cyclodiphosphate synthase mecps

Also Published As

Publication number Publication date
US20040072142A1 (en) 2004-04-15
US20090199313A1 (en) 2009-08-06
EP1287145B1 (en) 2008-11-26
WO2001094561A3 (en) 2002-05-30
US7288367B2 (en) 2007-10-30
DE10027821A1 (en) 2001-12-06
EP1287145A2 (en) 2003-03-05
DE60136701D1 (en) 2009-01-08
WO2001094561A2 (en) 2001-12-13
ATE415479T1 (en) 2008-12-15

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