AU2001268273A1 - Mutant proteinase with reduced self-cleavage activity and method of purification - Google Patents
Mutant proteinase with reduced self-cleavage activity and method of purificationInfo
- Publication number
- AU2001268273A1 AU2001268273A1 AU2001268273A AU6827301A AU2001268273A1 AU 2001268273 A1 AU2001268273 A1 AU 2001268273A1 AU 2001268273 A AU2001268273 A AU 2001268273A AU 6827301 A AU6827301 A AU 6827301A AU 2001268273 A1 AU2001268273 A1 AU 2001268273A1
- Authority
- AU
- Australia
- Prior art keywords
- purification
- cleavage activity
- reduced self
- mutant proteinase
- proteinase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
- C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/22044—Nuclear-inclusion-a endopeptidase (3.4.22.44)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US21153500P | 2000-06-15 | 2000-06-15 | |
US60211535 | 2000-06-15 | ||
PCT/US2001/018620 WO2001096539A2 (en) | 2000-06-15 | 2001-06-11 | Mutant nia proteinase with reduced self-cleavage activity and method of purification |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2001268273A1 true AU2001268273A1 (en) | 2001-12-24 |
Family
ID=22787327
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2001268273A Abandoned AU2001268273A1 (en) | 2000-06-15 | 2001-06-11 | Mutant proteinase with reduced self-cleavage activity and method of purification |
Country Status (4)
Country | Link |
---|---|
US (5) | US7494786B2 (en) |
AU (1) | AU2001268273A1 (en) |
CA (1) | CA2435381C (en) |
WO (1) | WO2001096539A2 (en) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2001268273A1 (en) | 2000-06-15 | 2001-12-24 | Yale University | Mutant proteinase with reduced self-cleavage activity and method of purification |
US7166436B2 (en) | 2001-01-26 | 2007-01-23 | Syngenta Participations, Ag | Differential labeling for quantitative analysis of complex protein mixtures |
US6969757B2 (en) | 2001-01-26 | 2005-11-29 | Syngenta Participations Ag | Differential labeling for quantitative analysis of complex protein mixtures |
WO2011038219A1 (en) * | 2009-09-24 | 2011-03-31 | Promega Corporation | Proteases having improved stability, solubility and activity |
CN101864407B (en) * | 2010-06-18 | 2016-02-10 | 安徽农业大学 | TEV protease mutant and encoding gene and application thereof |
WO2012011621A1 (en) * | 2010-07-23 | 2012-01-26 | Gwangju Institute Of Science And Technology | Amyloid-beta clearance |
KR101536963B1 (en) * | 2013-09-17 | 2015-07-16 | 재단법인 전주생물소재연구소 | Method for mass producing TEV protease in plant and TEV protease produced by the same |
US11661599B1 (en) | 2017-12-14 | 2023-05-30 | National Technology & Engineering Solutions Of Sandia, Llc | CRISPR-Cas based system for targeting single-stranded sequences |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5532142A (en) * | 1993-02-12 | 1996-07-02 | Board Of Regents, The University Of Texas System | Method of isolation and purification of fusion polypeptides |
AU2001268273A1 (en) | 2000-06-15 | 2001-12-24 | Yale University | Mutant proteinase with reduced self-cleavage activity and method of purification |
-
2001
- 2001-06-11 AU AU2001268273A patent/AU2001268273A1/en not_active Abandoned
- 2001-06-11 CA CA2435381A patent/CA2435381C/en not_active Expired - Lifetime
- 2001-06-11 US US10/343,766 patent/US7494786B2/en not_active Expired - Lifetime
- 2001-06-11 WO PCT/US2001/018620 patent/WO2001096539A2/en active Application Filing
-
2008
- 2008-08-01 US US12/184,315 patent/US8206968B2/en not_active Expired - Lifetime
-
2012
- 2012-05-29 US US13/482,535 patent/US8470575B2/en not_active Expired - Lifetime
-
2013
- 2013-05-21 US US13/898,650 patent/US8969062B2/en not_active Expired - Fee Related
-
2014
- 2014-12-11 US US14/567,368 patent/US20150152403A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO2001096539A3 (en) | 2002-05-16 |
US20040072179A1 (en) | 2004-04-15 |
US7494786B2 (en) | 2009-02-24 |
US8206968B2 (en) | 2012-06-26 |
US20150152403A1 (en) | 2015-06-04 |
CA2435381C (en) | 2013-09-17 |
US20080318273A1 (en) | 2008-12-25 |
US8969062B2 (en) | 2015-03-03 |
US8470575B2 (en) | 2013-06-25 |
WO2001096539A2 (en) | 2001-12-20 |
US20120252096A1 (en) | 2012-10-04 |
US20130337503A1 (en) | 2013-12-19 |
CA2435381A1 (en) | 2001-12-20 |
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