AU2001267844A1 - Method of preparing heart muscle cells and method of searching for remedy for heart diseases - Google Patents
Method of preparing heart muscle cells and method of searching for remedy for heart diseasesInfo
- Publication number
- AU2001267844A1 AU2001267844A1 AU2001267844A AU6784401A AU2001267844A1 AU 2001267844 A1 AU2001267844 A1 AU 2001267844A1 AU 2001267844 A AU2001267844 A AU 2001267844A AU 6784401 A AU6784401 A AU 6784401A AU 2001267844 A1 AU2001267844 A1 AU 2001267844A1
- Authority
- AU
- Australia
- Prior art keywords
- heart muscle
- heart
- muscle cells
- cells
- apoptosis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5061—Muscle cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0657—Cardiomyocytes; Heart cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/02—Drug screening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2510/00—Detection of programmed cell death, i.e. apoptosis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/32—Cardiovascular disorders
- G01N2800/324—Coronary artery diseases, e.g. angina pectoris, myocardial infarction
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Cardiology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- General Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Rheumatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention relates to a method of preparing primary heart muscle cells, characterized by washing a fine fragmented heart tissue with a phosphate-buffered physiological saline to thereby eliminate non-heart muscle cells and then hemolyzing the heart tissue digested with a protease to thereby eliminate erythrocytes. According to this method, highly pure primary heart muscle cells can be conveniently obtained in a large amount from the heart of an animal embryo or newborn. By using the heart muscle thus prepared, apoptosis of heart muscle cells can be efficiently and highly sensitively detected. Thus, it is possible to efficiently screen candidate compounds for heart muscle cell apoptosis inhibitors, gp 130-mediated receptor agonists, heart muscle cell-protective signal enhancers, preventives and remedies for heart diseases. A method of detecting apoptosis comprises inducing apoptosis of heart muscle cells by incubating the cells in a serum-free medium, adding serum to the liquid culture medium thereof, then incubating the cells and counting viable heart muscle cells.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2000-194805 | 2000-06-28 | ||
JP2000194805 | 2000-06-28 | ||
PCT/JP2001/005520 WO2002000850A1 (en) | 2000-06-28 | 2001-06-27 | Method of preparing heart muscle cells and method of searching for remedy for heart diseases |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2001267844A1 true AU2001267844A1 (en) | 2002-01-08 |
Family
ID=18693571
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2001267844A Abandoned AU2001267844A1 (en) | 2000-06-28 | 2001-06-27 | Method of preparing heart muscle cells and method of searching for remedy for heart diseases |
Country Status (6)
Country | Link |
---|---|
US (2) | US20030175847A1 (en) |
EP (2) | EP1295936B1 (en) |
AT (1) | ATE412766T1 (en) |
AU (1) | AU2001267844A1 (en) |
DE (1) | DE60136358D1 (en) |
WO (1) | WO2002000850A1 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004027014A1 (en) * | 2002-09-17 | 2004-04-01 | Japan Tissue Engineering Co.,Ltd | Cell dispersing apparatus, cell dispersion cassette and tissue retention part |
US8168718B2 (en) | 2005-12-09 | 2012-05-01 | Exxonmobil Chemical Patents Inc. | Thermoplastic vulcanizate adhesive compositions |
EP2226633A1 (en) * | 2009-03-06 | 2010-09-08 | Charité - Universitätsmedizin Berlin | Uses and methods for the identification of a cytoprotective compound involving gp130 or LIFRalpha |
CN108949673A (en) * | 2018-08-13 | 2018-12-07 | 武汉华联科生物技术有限公司 | A kind of primary separation method of Fetal Rat rat cardiomyocyte |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3948725A (en) * | 1974-05-30 | 1976-04-06 | Godo Shusei Kabushiki Kaisha | Neutral protease useful for animal tissue and cell culture |
JPH0372872A (en) * | 1988-07-12 | 1991-03-28 | Bio Kagaku Kenkyusho:Kk | Serum-free culture medium and method for culturing cell of mammals using the same |
US5543318A (en) * | 1991-06-12 | 1996-08-06 | Smith; David A. | Method of isolation, culture and proliferation of human atrial myocytes |
US5378698A (en) * | 1991-10-21 | 1995-01-03 | Shionogi & Co., Ltd. | Benzothiazepine derivatives |
US5919449A (en) * | 1995-05-30 | 1999-07-06 | Diacrin, Inc. | Porcine cardiomyocytes and their use in treatment of insufficient cardiac function |
US6133233A (en) * | 1997-02-18 | 2000-10-17 | Kansas State University Research Foundation | Peptide modulation of reperfusion injury |
WO1999011771A1 (en) * | 1997-09-04 | 1999-03-11 | Science Research Laboratory, Inc. | Cell separation using electric fields |
AU748334B2 (en) * | 1997-10-16 | 2002-05-30 | Board Of Regents, The University Of Texas System | Models and treatments for cardiac hypertrophy in relation with NF-AT3 function |
-
2001
- 2001-06-27 WO PCT/JP2001/005520 patent/WO2002000850A1/en active Application Filing
- 2001-06-27 US US10/311,119 patent/US20030175847A1/en not_active Abandoned
- 2001-06-27 EP EP01945643A patent/EP1295936B1/en not_active Expired - Lifetime
- 2001-06-27 AT AT01945643T patent/ATE412766T1/en not_active IP Right Cessation
- 2001-06-27 EP EP08075845A patent/EP2090663A3/en not_active Withdrawn
- 2001-06-27 DE DE60136358T patent/DE60136358D1/en not_active Expired - Lifetime
- 2001-06-27 AU AU2001267844A patent/AU2001267844A1/en not_active Abandoned
-
2008
- 2008-02-07 US US12/069,074 patent/US20080160553A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1295936A1 (en) | 2003-03-26 |
US20080160553A1 (en) | 2008-07-03 |
EP2090663A2 (en) | 2009-08-19 |
EP2090663A3 (en) | 2009-11-11 |
DE60136358D1 (en) | 2008-12-11 |
EP1295936A4 (en) | 2004-11-10 |
EP1295936B1 (en) | 2008-10-29 |
ATE412766T1 (en) | 2008-11-15 |
US20030175847A1 (en) | 2003-09-18 |
WO2002000850A1 (en) | 2002-01-03 |
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