AU2001256762A1 - Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby - Google Patents

Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby

Info

Publication number
AU2001256762A1
AU2001256762A1 AU2001256762A AU5676201A AU2001256762A1 AU 2001256762 A1 AU2001256762 A1 AU 2001256762A1 AU 2001256762 A AU2001256762 A AU 2001256762A AU 5676201 A AU5676201 A AU 5676201A AU 2001256762 A1 AU2001256762 A1 AU 2001256762A1
Authority
AU
Australia
Prior art keywords
sugar chain
reduction
protein
glycoprotein
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001256762A
Inventor
Masaaki Hirose
Hiroyuki Irie
Shinobu Kuwae
Masami Miura
Taeko Miwa
Kenmi Miyano
Hideyuki Ohi
Wataru Otani
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Pharma Corp
Original Assignee
Welfide Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Welfide Corp filed Critical Welfide Corp
Publication of AU2001256762A1 publication Critical patent/AU2001256762A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

The present invention intends to find out a gene participating in addition of mannose phosphate to a sugar chain of a glycoprotein originating in a yeast belonging to the genus Pichia and provide a means of controlling the same. The present invention also intends to provide a process for producing a protein with reduction of an acidic sugar chain by using the thus controlled yeast strain belonging to the genus Pichia. Namely, the present invention includes a protein participating in the addition of mannose phosphate to a sugar chain of a glycoprotein; a gene coding this protein; a mutant of this gene; a vector carrying the mutant gene; a yeast strain belonging to the genus Pichia having been transformed by this vector; a process for producing a protein with reduction of an acidic sugar chain by using the transformed yeast strain; and a glycoprotein thus produced.
AU2001256762A 2000-05-17 2001-05-16 Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby Abandoned AU2001256762A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2000-144643 2000-05-17
JP2000144643 2000-05-17
PCT/JP2001/004070 WO2001088143A1 (en) 2000-05-17 2001-05-16 Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby

Publications (1)

Publication Number Publication Date
AU2001256762A1 true AU2001256762A1 (en) 2001-11-26

Family

ID=18651270

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001256762A Abandoned AU2001256762A1 (en) 2000-05-17 2001-05-16 Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby

Country Status (9)

Country Link
US (3) US7198921B2 (en)
EP (1) EP1283265B1 (en)
JP (2) JP4969011B2 (en)
AT (1) ATE438722T1 (en)
AU (1) AU2001256762A1 (en)
DE (1) DE60139467D1 (en)
DK (1) DK1283265T3 (en)
ES (1) ES2328106T3 (en)
WO (1) WO2001088143A1 (en)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001256762A1 (en) * 2000-05-17 2001-11-26 Mitsubishi Pharma Corporation Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby
WO2002057468A2 (en) 2001-01-19 2002-07-25 The Dow Chemical Company Method for secretory production of glycoprotein having human-type sugar chain using plant cell
US7259007B2 (en) 2003-12-24 2007-08-21 Glycofi, Inc. Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins
EP1747280B1 (en) 2004-04-29 2018-01-17 GlycoFi, Inc. Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins
JP2009509970A (en) * 2005-09-22 2009-03-12 プロサイ インコーポレイテッド Glycosylated polypeptides produced in yeast mutants and methods of use thereof
EP2134834B1 (en) * 2007-03-07 2013-04-17 GlycoFi, Inc. Production of glycoproteins with modified fucosylation
CN104480140B (en) * 2007-04-03 2019-12-31 奥克西雷恩英国有限公司 Glycosylation of molecules
EP2147108B1 (en) 2007-04-17 2014-03-05 Stichting Dienst Landbouwkundig Onderzoek Mammalian-type glycosylation in plants by expression of non-mammalian glycosyltransferases
US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
CA2705925C (en) * 2007-12-19 2019-03-12 Glycofi, Inc. Yeast strains for protein production
EP2245151B1 (en) 2008-02-20 2014-12-24 GlycoFi, Inc. Vectors and yeast strains for protein production
JP5731827B2 (en) 2008-03-03 2015-06-10 グライコフィ， インコーポレイテッド Surface display of recombinant proteins in lower eukaryotes
US8067339B2 (en) 2008-07-09 2011-11-29 Merck Sharp & Dohme Corp. Surface display of whole antibodies in eukaryotes
MX2011001706A (en) 2008-08-12 2011-03-24 Glycofi Inc Improved vectors and yeast strains for protein production: ca2+ atpase overexpression.
US20120003695A1 (en) 2009-02-25 2012-01-05 Davidson Robert C Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris
CN104651428A (en) 2009-09-29 2015-05-27 根特大学 Hydrolysis of mannose-1-phospho-6-mannose linkage to phospho-6-mannose
AU2010307098A1 (en) 2009-10-16 2012-04-26 Merck Sharp & Dohme Corp. Method for producing proteins in Pichia pastoris that lack detectable cross binding activity to antibodies against host cell antigens
JP2013509181A (en) 2009-10-30 2013-03-14 メルク・シャープ・エンド・ドーム・コーポレイション Process for producing therapeutic proteins in Pichia pastoris lacking dipeptidylaminopeptidase activity
US20130011875A1 (en) 2009-10-30 2013-01-10 Merck Sharpe & Dohme Corp Methods for the production of recombinant proteins with improved secretion efficiencies
BR112012011980A2 (en) 2009-11-19 2021-09-08 Oxyrane Uk Limited METHODS OF PRODUCING TRANSFORMED YARROWIA LIPOLYTICA CELLS AND TARGET PROTEINS INCLUDING N-GLYCANS, TRANSFORMED YARROWIA LIPOLYTICA CELLS AND THEIR CULTURES, AS WELL AS A COMPOSITION COMPRISING GLYCOPROTEINS
CN102858949B (en) 2010-02-24 2016-07-06 默沙东公司 For increasing the method that the N-glycosylation site on the therapeutic glycoprotein produced in pichia pastoris occupies
CA2799595C (en) 2010-05-27 2022-08-16 Merck Sharp & Dohme Corp. Method for preparing antibodies having improved properties
CN103328646B (en) 2010-09-29 2021-06-18 奥克西雷恩英国有限公司 Mannosidase capable of uncapping mannose-1-phosphate-6-mannose linkages and demannosylating phosphorylated N-glycans and a method of promoting mammalian cellular uptake of glycoproteins
US9689015B2 (en) 2010-09-29 2017-06-27 Oxyrane Uk Limited De-mannosylation of phosphorylated N-glycans
EP2646606B1 (en) 2010-12-01 2018-10-10 Merck Sharp&Dohme Corp. Surface-anchored fc-bait antibody display system
AU2012220890A1 (en) 2011-02-25 2013-08-29 Merck Sharp & Dohme Corp. Yeast strain for the production of proteins with modified O-glycosylation
CN103582650A (en) 2011-05-25 2014-02-12 默沙东公司 Method for preparing Fc-containing polypeptides having improved properties
WO2013062940A1 (en) 2011-10-28 2013-05-02 Merck Sharp & Dohme Corp. Engineered lower eukaryotic host strains for recombinant protein expression
US9249399B2 (en) 2012-03-15 2016-02-02 Oxyrane Uk Limited Methods and materials for treatment of pompe's disease
US9890378B2 (en) 2012-05-11 2018-02-13 Merck Sharp & Dohme Corp. Surface anchored light chain bait antibody display system
EP2852610B1 (en) 2012-05-23 2018-07-11 Glykos Finland Oy Production of fucosylated glycoproteins
EP2909306B1 (en) 2012-10-22 2019-12-11 Merck Sharp & Dohme Corp. Crz1 mutant fungal cells
US9707276B2 (en) 2012-12-03 2017-07-18 Merck Sharp & Dohme Corp. O-glycosylated carboxy terminal portion (CTP) peptide-based insulin and insulin analogues
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL82935A0 (en) 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast
JPH093097A (en) 1995-06-16 1997-01-07 Green Cross Corp:The Saccharide chain elongation protein derived from genus pichia, dna coding the same protein, modified dna and transformant
JP2770010B2 (en) 1996-03-29 1998-06-25 工業技術院長 Gene for positively controlling mannose-1-phosphate transfer in yeast and method for producing high mannose-type neutral sugar chain using mutant mutant of this gene
US6747137B1 (en) * 1998-02-13 2004-06-08 Genome Therapeutics Corporation Nucleic acid sequences relating to Candida albicans for diagnostics and therapeutics
AU2001256762A1 (en) 2000-05-17 2001-11-26 Mitsubishi Pharma Corporation Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby
DE60139720D1 (en) * 2000-06-28 2009-10-08 Glycofi Inc Process for the preparation of modified glycoproteins

Also Published As

Publication number Publication date
US7872111B2 (en) 2011-01-18
JP4969011B2 (en) 2012-07-04
DK1283265T3 (en) 2009-11-09
WO2001088143A1 (en) 2001-11-22
US20070202569A1 (en) 2007-08-30
EP1283265A1 (en) 2003-02-12
US7198921B2 (en) 2007-04-03
ATE438722T1 (en) 2009-08-15
JP2012105652A (en) 2012-06-07
US20090004725A1 (en) 2009-01-01
US20040014170A1 (en) 2004-01-22
US7488591B2 (en) 2009-02-10
EP1283265A4 (en) 2004-10-20
ES2328106T3 (en) 2009-11-10
DE60139467D1 (en) 2009-09-17
EP1283265B1 (en) 2009-08-05

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