AU1398988A - Qualitative and quantitative determination of particulate antigens, in particular spores of clostridium tyrobutyricum - Google Patents

Qualitative and quantitative determination of particulate antigens, in particular spores of clostridium tyrobutyricum

Info

Publication number
AU1398988A
AU1398988A AU13989/88A AU1398988A AU1398988A AU 1398988 A AU1398988 A AU 1398988A AU 13989/88 A AU13989/88 A AU 13989/88A AU 1398988 A AU1398988 A AU 1398988A AU 1398988 A AU1398988 A AU 1398988A
Authority
AU
Australia
Prior art keywords
qualitative
quantitative determination
particulate antigens
clostridium tyrobutyricum
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU13989/88A
Inventor
Bertrand Favreau
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biosys SA
Original Assignee
Biosys SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biosys SA filed Critical Biosys SA
Publication of AU1398988A publication Critical patent/AU1398988A/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A process for detecting and counting particulate antigens in a liquid sample consists in: a) coupling the said antigens with one or more antibodies at least one of which is coupled to a first enzyme; 6) depositing the antigen-antibody complexes so formed on a gel containing the substrate of the first enzyme and a second enzyme, the substrate of which is the product of the first enzymatic reaction; the said gel also containing a developer - by formation of a precipitate - of the second enzymatic reactions; c) observing and/or counting the spots which appear on the gel.
AU13989/88A 1987-03-03 1988-03-02 Qualitative and quantitative determination of particulate antigens, in particular spores of clostridium tyrobutyricum Abandoned AU1398988A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8702862 1987-03-03
FR8702862A FR2611912B1 (en) 1987-03-03 1987-03-03 METHOD FOR THE DETECTION AND ENUMERATION OF PARTICULATE ANTIGENS IN A LIQUID SAMPLE, PARTICULARLY SPORES OF CLOSTRIDIUM TYROBUTYRICUM IN MILK

Publications (1)

Publication Number Publication Date
AU1398988A true AU1398988A (en) 1988-09-26

Family

ID=9348547

Family Applications (1)

Application Number Title Priority Date Filing Date
AU13989/88A Abandoned AU1398988A (en) 1987-03-03 1988-03-02 Qualitative and quantitative determination of particulate antigens, in particular spores of clostridium tyrobutyricum

Country Status (7)

Country Link
EP (1) EP0281477B1 (en)
AT (1) ATE78599T1 (en)
AU (1) AU1398988A (en)
DE (1) DE3872900T2 (en)
FR (1) FR2611912B1 (en)
NO (1) NO173714C (en)
WO (1) WO1988006734A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2123507T3 (en) * 1991-11-28 1999-01-16 Solutia Europ Nv Sa PROCEDURE FOR THE PULTRUSION OF NYLON.
IES940182A2 (en) * 1994-03-01 1995-11-29 Teagasc Agric Food Dev Authori "Rapid detection of bacteria in liquid cultures"
ES2339912B1 (en) * 2008-11-25 2011-03-22 Universidad De Zaragoza METHOD FOR THE DETECTION AND / OR QUANTIFICATION OF CLOSTRIDIUMTYROBUTYRICUM SPORTS BY FLOW CYTOMETRY.

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2273280A1 (en) * 1974-05-31 1975-12-26 Anvar Measurement of antigens and antibodies - in biological fluids by immuno diffusion techniques with antibodies marked with enzymes
US4435504A (en) * 1982-07-15 1984-03-06 Syva Company Immunochromatographic assay with support having bound "MIP" and second enzyme
US4652533A (en) * 1983-04-28 1987-03-24 Pandex Laboratories, Inc. Method of solid phase immunoassay incorporating a luminescent label
US4562147A (en) * 1983-06-13 1985-12-31 Regents Of The University Of Minnesota Method and kit for diagnosis of pseudorabies
GB8404368D0 (en) * 1984-02-20 1984-03-28 Cogent Ltd Monoclonal antibodies to human cytomegalovirus

Also Published As

Publication number Publication date
NO884878L (en) 1988-11-02
EP0281477A1 (en) 1988-09-07
FR2611912B1 (en) 1989-07-13
FR2611912A1 (en) 1988-09-09
NO173714C (en) 1994-01-19
WO1988006734A1 (en) 1988-09-07
ATE78599T1 (en) 1992-08-15
NO173714B (en) 1993-10-11
DE3872900D1 (en) 1992-08-27
EP0281477B1 (en) 1992-07-22
DE3872900T2 (en) 1993-03-04
NO884878D0 (en) 1988-11-02

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