ATE7702T1 - Verfahren zum entfernen von trityl-schutzgruppen aus 5'-o-trityl-nukleosiden und oligonukleotiden. - Google Patents

Verfahren zum entfernen von trityl-schutzgruppen aus 5'-o-trityl-nukleosiden und oligonukleotiden.

Info

Publication number
ATE7702T1
ATE7702T1 AT81101444T AT81101444T ATE7702T1 AT E7702 T1 ATE7702 T1 AT E7702T1 AT 81101444 T AT81101444 T AT 81101444T AT 81101444 T AT81101444 T AT 81101444T AT E7702 T1 ATE7702 T1 AT E7702T1
Authority
AT
Austria
Prior art keywords
nucleoside
polymer
trityl
oligonucleotides
modified
Prior art date
Application number
AT81101444T
Other languages
English (en)
Inventor
Marvin H. Caruthers
Mark D. Matteucci
Original Assignee
University Patents, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Patents, Inc. filed Critical University Patents, Inc.
Application granted granted Critical
Publication of ATE7702T1 publication Critical patent/ATE7702T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H23/00—Compounds containing boron, silicon, or a metal, e.g. chelates, vitamin B12
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00281—Individual reactor vessels
    • B01J2219/00286—Reactor vessels with top and bottom openings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00389—Feeding through valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Saccharide Compounds (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
AT81101444T 1980-02-29 1981-02-27 Verfahren zum entfernen von trityl-schutzgruppen aus 5'-o-trityl-nukleosiden und oligonukleotiden. ATE7702T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12602580A 1980-02-29 1980-02-29
EP81101444A EP0035255B1 (de) 1980-02-29 1981-02-27 Verfahren zum Entfernen von Trityl-Schutzgruppen aus 5'-O-trityl-Nukleosiden und Oligonukleotiden

Publications (1)

Publication Number Publication Date
ATE7702T1 true ATE7702T1 (de) 1984-06-15

Family

ID=22422602

Family Applications (2)

Application Number Title Priority Date Filing Date
AT81101444T ATE7702T1 (de) 1980-02-29 1981-02-27 Verfahren zum entfernen von trityl-schutzgruppen aus 5'-o-trityl-nukleosiden und oligonukleotiden.
AT85200673T ATE56723T1 (de) 1980-02-29 1981-02-27 Verfahren zur herstellung von modifizierten anorganischen polymeren.

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT85200673T ATE56723T1 (de) 1980-02-29 1981-02-27 Verfahren zur herstellung von modifizierten anorganischen polymeren.

Country Status (7)

Country Link
EP (4) EP0035255B1 (de)
JP (4) JPS56133299A (de)
AT (2) ATE7702T1 (de)
AU (2) AU542967B2 (de)
CA (2) CA1159052A (de)
DE (2) DE3163811D1 (de)
MX (1) MX158743A (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0073826A1 (de) * 1981-03-10 1983-03-16 Bioresearch Inc. Schnelle herstellung in fester phase von oligonukleotiden unter verwendung von phosphoroxychlorid
US4415732A (en) * 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
JPS58148896A (ja) * 1982-02-26 1983-09-05 Shimadzu Corp Ｄｎａ等微量自動合成装置
DE3208565A1 (de) * 1982-03-10 1983-11-10 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur abspaltung von tritylschutzgruppen
EP0090789A1 (de) * 1982-03-26 1983-10-05 Monsanto Company Chemische DNA-Synthese
US4474948A (en) * 1982-04-08 1984-10-02 Biosearch Benzazolides and their employment in phosphite ester oligonucleotide synthesis processes
IL69196A0 (en) * 1982-08-20 1983-11-30 Genex Corp Solid phase synthesis of oligonucleotides
WO1984002526A1 (en) * 1982-12-29 1984-07-05 Univ Southern Illinois Process for producing polynucleotides
FR2544720B1 (fr) * 1983-04-19 1987-11-27 California Inst Of Techn Installation perfectionnee et procede pour la synthese de composes chimiques, en particulier d'oligonucleotides
DE3329892A1 (de) 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
US5539097A (en) * 1983-09-02 1996-07-23 Molecular Biosystems, Inc. Oligonucleotide polymeric support system
JPS61152695A (ja) * 1984-12-26 1986-07-11 Nippon Shinyaku Co Ltd 長鎖ｄｎａの合成法
US4818681A (en) * 1985-02-22 1989-04-04 Molecular Diagnostics, Inc. Fast and specific immobilization of nucleic acids to solid supports
WO1986007362A1 (en) * 1985-06-14 1986-12-18 University Patents, Inc. Method for synthesizing deoxyoligonucleotides
FR2596761B1 (fr) * 1986-04-08 1988-05-20 Commissariat Energie Atomique Derives de nucleosides et leur utilisation pour la synthese d'oligonucleotides
US5071974A (en) * 1986-10-31 1991-12-10 Amoco Corporation Compositions and methods for the synthesis of oligonucleotides having 5'-phosphorylated termini
DE3801987A1 (de) * 1988-01-23 1989-07-27 Boehringer Mannheim Gmbh Traeger zur chemischen oder/und enzymatischen umsetzung von nukleinsaeuren oder nukleinsaeurefragmenten an festphasen
SE9003743D0 (sv) * 1990-11-26 1990-11-26 Pharmacia Ab Method and means for oligonucleotide synthesis
CA2114355A1 (en) * 1993-01-29 1994-07-30 Hidehiko Furukawa Modified oligodeoxyribonucleotides, their preparation and their therapeutic use
US5503816A (en) * 1993-09-27 1996-04-02 Becton Dickinson And Company Silicate compounds for DNA purification
FR2714061B1 (fr) 1993-12-16 1996-03-08 Genset Sa Procédé de préparation de polynucléotides sur support solide et appareil permettant sa mise en Óoeuvre.
US5874554A (en) * 1996-12-13 1999-02-23 Incyte Pharmaceuticals, Inc. Methods and solvent vehicles for reagent delivery in oligonucleotide synthesis using automated pulse jetting devices
DE69825722T2 (de) * 1998-02-04 2005-08-25 Corning Inc. Substrat zum Drucken einer Matrize
US6399765B1 (en) * 1999-03-17 2002-06-04 Isis Pharmaceuticals, Inc. Methods for removing dimethoxytrityl groups from oligonucleotides
ES2250221T3 (es) * 1999-11-10 2006-04-16 Merckle Gmbh Procedimiento y dispositivo par ala preparacion de oligomeros y conjuntos de oligomeros asi como el uso del dispositivo.
CN1471537A (zh) * 2000-09-07 2004-01-28 ƽ 用于低聚核苷酸合成的合成子
KR101137736B1 (ko) 2004-09-30 2012-04-24 각코호진 와세다다이가쿠 반도체 센싱용 전계 효과형 트랜지스터, 반도체 센싱디바이스, 반도체 센서 칩 및 반도체 센싱 장치
US7396676B2 (en) 2005-05-31 2008-07-08 Agilent Technologies, Inc. Evanescent wave sensor with attached ligand
EP1896455A2 (de) * 2005-06-06 2008-03-12 Medichem, S.A. Verfahren zur herstellung von tetrazolylverbindungen

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1113937B (de) * 1959-11-13 1961-09-21 Shell Int Research Verfahren zur Herstellung von heterocyclischen Amiden der Phosphorsaeure
US3557077A (en) * 1967-09-18 1971-01-19 Kay Brunfeldt Reactions system
US3531258A (en) * 1967-11-16 1970-09-29 Us Health Education & Welfare Apparatus for the automated synthesis of peptides
IL62834A0 (en) * 1980-05-14 1981-07-31 Ens Bio Logicals Inc Polynucleotide synthesis
US4415732A (en) * 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes

Also Published As

Publication number Publication date
EP0173356A3 (en) 1987-01-14
EP0035719A3 (en) 1981-11-18
AU550148B2 (en) 1986-03-06
ATE56723T1 (de) 1990-10-15
EP0097805A3 (de) 1984-02-22
JPS6330316B2 (de) 1988-06-17
JPH0327395A (ja) 1991-02-05
EP0173356A2 (de) 1986-03-05
JPH0259158B2 (de) 1990-12-11
AU6794381A (en) 1981-09-03
JPS63225395A (ja) 1988-09-20
CA1159052A (en) 1983-12-20
AU6794281A (en) 1981-09-03
AU542967B2 (en) 1985-03-28
EP0097805A2 (de) 1984-01-11
EP0035255A1 (de) 1981-09-09
JPS56138199A (en) 1981-10-28
MX158743A (es) 1989-03-10
EP0035255B1 (de) 1984-05-30
CA1168229A (en) 1984-05-29
EP0173356B1 (de) 1990-09-19
EP0035719A2 (de) 1981-09-16
DE3177215D1 (de) 1990-10-25
JPS56133299A (en) 1981-10-19
DE3163811D1 (en) 1984-07-05
EP0035719B1 (de) 1989-09-20

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EELA Cancelled due to lapse of time