ATE542902T1 - ISOLATION AND SEQUENCING OF THE GENE PKNB FROM C. GLUTAMICUM - Google Patents

ISOLATION AND SEQUENCING OF THE GENE PKNB FROM C. GLUTAMICUM

Info

Publication number
ATE542902T1
ATE542902T1 AT01960723T AT01960723T ATE542902T1 AT E542902 T1 ATE542902 T1 AT E542902T1 AT 01960723 T AT01960723 T AT 01960723T AT 01960723 T AT01960723 T AT 01960723T AT E542902 T1 ATE542902 T1 AT E542902T1
Authority
AT
Austria
Prior art keywords
glutamicum
pknb
gene
sequencing
isolation
Prior art date
Application number
AT01960723T
Other languages
German (de)
Inventor
Brigitte Bathe
Stephan Hans
Mike Farwick
Thomas Hermann
Original Assignee
Evonik Degussa Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evonik Degussa Gmbh filed Critical Evonik Degussa Gmbh
Priority claimed from PCT/EP2001/010211 external-priority patent/WO2002022828A1/en
Application granted granted Critical
Publication of ATE542902T1 publication Critical patent/ATE542902T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01037—Protein kinase (2.7.1.37)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to the protein kinase B of C. glutamicum and a fermentation process for the preparation of L-amino acids using corynebacteria in which at least the pknB gene is amplified, and to the use, as hybridization probes, of polynucleotides containing the sequences according to the invention.
AT01960723T 2000-09-12 2001-09-05 ISOLATION AND SEQUENCING OF THE GENE PKNB FROM C. GLUTAMICUM ATE542902T1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10044912 2000-09-12
DE10120095A DE10120095A1 (en) 2000-09-12 2001-04-25 New nucleotide sequences coding for the pknB gene
PCT/EP2001/010211 WO2002022828A1 (en) 2000-09-12 2001-09-05 Isolation and sequencing of the pknb gene of c. glutamicum

Publications (1)

Publication Number Publication Date
ATE542902T1 true ATE542902T1 (en) 2012-02-15

Family

ID=7655827

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01960723T ATE542902T1 (en) 2000-09-12 2001-09-05 ISOLATION AND SEQUENCING OF THE GENE PKNB FROM C. GLUTAMICUM

Country Status (2)

Country Link
AT (1) ATE542902T1 (en)
DE (1) DE10120095A1 (en)

Also Published As

Publication number Publication date
DE10120095A1 (en) 2002-03-28

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