ATE538203T1 - DNA CODING FOR POLYPEPTIDE INVOLVED IN THE BIOSYNTHESIS OF PLADIENOLIDE - Google Patents

DNA CODING FOR POLYPEPTIDE INVOLVED IN THE BIOSYNTHESIS OF PLADIENOLIDE

Info

Publication number
ATE538203T1
ATE538203T1 AT05766205T AT05766205T ATE538203T1 AT E538203 T1 ATE538203 T1 AT E538203T1 AT 05766205 T AT05766205 T AT 05766205T AT 05766205 T AT05766205 T AT 05766205T AT E538203 T1 ATE538203 T1 AT E538203T1
Authority
AT
Austria
Prior art keywords
pladienolide
dna
biosynthesis
dna coding
polypeptide involved
Prior art date
Application number
AT05766205T
Other languages
German (de)
Inventor
Kazuhiro Machida
Akira Arisawa
Susumu Takeda
Masashi Yoshida
Toshio Tsuchida
Original Assignee
Eisai R&D Man Co Ltd
Mercian Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eisai R&D Man Co Ltd, Mercian Corp filed Critical Eisai R&D Man Co Ltd
Application granted granted Critical
Publication of ATE538203T1 publication Critical patent/ATE538203T1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/08—Oxygen as only ring hetero atoms containing a hetero ring of at least seven ring members, e.g. zearalenone, macrolide aglycons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
    • C12P17/162—Heterorings having oxygen atoms as the only ring heteroatoms, e.g. Lasalocid

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention provides polypeptides that participate in the biosynthesis of the pladienolide macrolide compounds, DNA that encodes these polypeptides and variants of this DNA, transformants that maintain all or a portion of this DNA or variant thereof, and a method of producing the pladienolide macrolide compounds using these transformants. More particularly, it provides an isolated pure DNA that contains at least one region encoding a polypeptide that participates in pladienolide biosynthesis; polypeptide encoded by this DNA; a self-replicating or integrated-replicating recombinant plasmid carrying this DNA; a transformant maintaining this DNA; and a method of producing a pladienolide, characterized by culturing this transformant on culture medium and collecting pladienolide from this culture medium.
AT05766205T 2004-07-20 2005-07-19 DNA CODING FOR POLYPEPTIDE INVOLVED IN THE BIOSYNTHESIS OF PLADIENOLIDE ATE538203T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2004211279 2004-07-20
PCT/JP2005/013541 WO2006009276A1 (en) 2004-07-20 2005-07-19 Dna coding for polypeptide participating in biosynthesis of pladienolide

Publications (1)

Publication Number Publication Date
ATE538203T1 true ATE538203T1 (en) 2012-01-15

Family

ID=35785378

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05766205T ATE538203T1 (en) 2004-07-20 2005-07-19 DNA CODING FOR POLYPEPTIDE INVOLVED IN THE BIOSYNTHESIS OF PLADIENOLIDE

Country Status (10)

Country Link
US (1) US8008049B2 (en)
EP (1) EP1770165B1 (en)
JP (1) JP4599357B2 (en)
KR (1) KR100834488B1 (en)
CN (1) CN1977046A (en)
AT (1) ATE538203T1 (en)
AU (1) AU2005265443B2 (en)
CA (1) CA2574092C (en)
IL (1) IL180167A0 (en)
WO (1) WO2006009276A1 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2546614A1 (en) 2003-11-27 2005-06-09 Mercian Corporation Dna participating in hydroxylation of macrolide compound
EP1770165B1 (en) 2004-07-20 2011-12-21 Eisai R&D Management Co., Ltd. Dna coding for polypeptide participating in biosynthesis of pladienolide
TW200716744A (en) * 2005-05-26 2007-05-01 Eisai R&D Man Co Ltd Genetically modified microorganism and process for production of macrolide compound using the microorganism
WO2007043621A1 (en) 2005-10-13 2007-04-19 Eisai R & D Management Co., Ltd. Total synthesis of pladienolide b and pladienolide d
WO2009147984A1 (en) 2008-06-04 2009-12-10 エーザイ・アール・アンド・ディー・マネジメント株式会社 Dna encoding polypeptide involved in biosynthesis of herboxidiene
US8865761B1 (en) 2012-08-07 2014-10-21 The University Of Notre Dame Du Lac Regulation of cholesterol homeostasis
KR102146726B1 (en) 2014-05-15 2020-08-21 에자이 알앤드디 매니지먼트 가부시키가이샤 Pladienolide pyridine compounds and methods of use
ES2887201T3 (en) 2015-09-01 2021-12-22 Eisai R&D Man Co Ltd Splice variants associated with neomorphic mutants of SF3B1
WO2017087667A1 (en) 2015-11-18 2017-05-26 Eisai R&D Management Co., Ltd. A solid state form of pladienolide pyridine compounds and methods of use
AU2018235940A1 (en) 2017-03-15 2019-10-17 Eisai R&D Management Co., Ltd. Spliceosome mutations and uses thereof
US11524009B2 (en) 2017-10-31 2022-12-13 Eisai R&D Management Co., Ltd. Combination comprising at least one spliceosome modulator and at least one inhibitor chosen from BCL2 inhibitors, BCL2/BCLxL inhibitors, and BCLxL inhibitors and methods of use
MX2020010606A (en) 2018-04-09 2021-01-15 Eisai R&D Man Co Ltd Pladienolide compounds and their use.
AU2019252432B2 (en) 2018-04-12 2023-04-13 Eisai R&D Management Co., Ltd. Pladienolide derivatives as spliceosome targeting agents for treating cancer
AU2019279012A1 (en) 2018-06-01 2020-12-24 Eisai R&D Management Co., Ltd. Methods of using splicing modulators
JP2023553588A (en) 2020-11-04 2023-12-25 エーザイ・アール・アンド・ディー・マネジメント株式会社 Biomarkers for myelodysplastic syndrome (MDS) and their use
WO2023131866A1 (en) 2022-01-05 2023-07-13 Eisai R&D Management Co., Ltd. Biomarkers for myelodysplastic syndrome (mds) and methods of using the same

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US5824513A (en) 1991-01-17 1998-10-20 Abbott Laboratories Recombinant DNA method for producing erythromycin analogs
WO1993013663A1 (en) * 1992-01-17 1993-07-22 Abbott Laboratories Method of directing biosynthesis of specific polyketides
JPH07502650A (en) 1991-12-16 1995-03-23 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー Constitutive expression of P450soy and ferredoxin-soy in Streptomyces and biotransformation of chemicals by recombinant organisms
ATE346938T1 (en) 1998-10-02 2006-12-15 Kosan Biosciences Inc POLYKETIDE SYNTHASE ENZYMES AND RECOMBINANT DNA CONSTRUCTS FOR PRODUCING COMPOUNDS RELATED TO FK-506 AND FK-520
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CA2546614A1 (en) 2003-11-27 2005-06-09 Mercian Corporation Dna participating in hydroxylation of macrolide compound
EP1770165B1 (en) 2004-07-20 2011-12-21 Eisai R&D Management Co., Ltd. Dna coding for polypeptide participating in biosynthesis of pladienolide
TW200716744A (en) 2005-05-26 2007-05-01 Eisai R&D Man Co Ltd Genetically modified microorganism and process for production of macrolide compound using the microorganism

Also Published As

Publication number Publication date
CA2574092C (en) 2011-08-23
JPWO2006009276A1 (en) 2008-05-01
KR100834488B1 (en) 2008-06-09
AU2005265443A1 (en) 2006-01-26
IL180167A0 (en) 2007-06-03
EP1770165A4 (en) 2008-11-12
US20090269820A1 (en) 2009-10-29
EP1770165B1 (en) 2011-12-21
JP4599357B2 (en) 2010-12-15
CA2574092A1 (en) 2006-01-26
US8008049B2 (en) 2011-08-30
AU2005265443B2 (en) 2008-09-04
KR20070033979A (en) 2007-03-27
EP1770165A1 (en) 2007-04-04
WO2006009276A1 (en) 2006-01-26
CN1977046A (en) 2007-06-06

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