ATE529534T1 - Verfahren unter verwendung eines oligonukleotids mit doppelspezifität und oligonukleotid mit doppelspezifität - Google Patents
Verfahren unter verwendung eines oligonukleotids mit doppelspezifität und oligonukleotid mit doppelspezifitätInfo
- Publication number
- ATE529534T1 ATE529534T1 AT06716196T AT06716196T ATE529534T1 AT E529534 T1 ATE529534 T1 AT E529534T1 AT 06716196 T AT06716196 T AT 06716196T AT 06716196 T AT06716196 T AT 06716196T AT E529534 T1 ATE529534 T1 AT E529534T1
- Authority
- AT
- Austria
- Prior art keywords
- dual specificity
- specificity oligonucleotide
- oligonucleotide
- dual
- present
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/107—Temperature of melting, i.e. Tm
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR20050018419 | 2005-03-05 | ||
PCT/KR2005/001206 WO2006095941A1 (en) | 2005-03-05 | 2005-04-26 | Processes using dual specificity oligonucleotide and dual specificity oligonucleotide |
PCT/KR2006/000746 WO2006095981A1 (en) | 2005-03-05 | 2006-03-03 | Processes using dual specificity oligonucleotide and dual specificity oligonucleotide |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE529534T1 true ATE529534T1 (de) | 2011-11-15 |
Family
ID=36953514
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT06716196T ATE529534T1 (de) | 2005-03-05 | 2006-03-03 | Verfahren unter verwendung eines oligonukleotids mit doppelspezifität und oligonukleotid mit doppelspezifität |
Country Status (20)
Country | Link |
---|---|
US (4) | US8092997B2 (de) |
EP (1) | EP2365078B1 (de) |
JP (1) | JP4903725B2 (de) |
KR (3) | KR101534228B1 (de) |
CN (1) | CN101189336B (de) |
AT (1) | ATE529534T1 (de) |
AU (1) | AU2006221202B2 (de) |
BR (1) | BRPI0609031B1 (de) |
CA (1) | CA2599647C (de) |
DK (2) | DK2365078T3 (de) |
ES (2) | ES2447297T3 (de) |
IL (1) | IL185683A (de) |
MX (1) | MX2007010875A (de) |
MY (1) | MY162156A (de) |
NO (2) | NO339577B1 (de) |
NZ (1) | NZ561166A (de) |
PL (1) | PL2365078T3 (de) |
PT (1) | PT2365078E (de) |
WO (1) | WO2006095941A1 (de) |
ZA (1) | ZA200707514B (de) |
Families Citing this family (34)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2006095941A1 (en) | 2005-03-05 | 2006-09-14 | Seegene, Inc. | Processes using dual specificity oligonucleotide and dual specificity oligonucleotide |
JP5197579B2 (ja) * | 2006-05-04 | 2013-05-15 | シージーン アイエヌシー | 既知の配列に隣接した未知のdna配列を増幅する方法 |
CN101903535A (zh) | 2007-03-28 | 2010-12-01 | 信号诊断公司 | 高解析度分析核酸以检测序列变异的***和方法 |
WO2010026450A1 (en) * | 2008-09-03 | 2010-03-11 | Quantumdx Group Limited | Sensing strategies and methods for nucleic acid detection using biosensors |
US10669574B2 (en) * | 2008-11-18 | 2020-06-02 | XCR Diagnostics, Inc. | DNA amplification technology |
EP2389585A2 (de) * | 2009-01-22 | 2011-11-30 | Li-Cor, Inc. | Einzelmolekül-proteomik mit dynamischen sonden |
WO2011027966A2 (en) * | 2009-09-03 | 2011-03-10 | Seegene, Inc. | Td probe and its uses |
KR101590175B1 (ko) * | 2009-09-24 | 2016-01-29 | 주식회사 씨젠 | 반복적 엑소핵산 절단 반응에 의한 타겟 핵산서열의 검출 |
KR20110050327A (ko) * | 2009-11-07 | 2011-05-13 | 주식회사 씨젠 | Thd 프라이머 타겟 검출 |
EP3246332B1 (de) * | 2010-01-12 | 2019-07-10 | Siemens Healthcare Diagnostics Inc. | Oligonukleotide und verfahren zur erkennung von pik3ca-mutationen |
JP2013081448A (ja) * | 2011-05-02 | 2013-05-09 | Arkray Inc | 遺伝子変異検出用プローブ |
EP2705162B1 (de) | 2011-05-04 | 2018-07-11 | Biocept, Inc. | Verfahren zur erkennung von nukleinsäuresequenzvarianten |
GB201116131D0 (en) * | 2011-09-19 | 2011-11-02 | Epistem Ltd | Probe |
CN104388426B (zh) * | 2012-02-28 | 2017-08-08 | 盛司潼 | 一种Oligo dT引物及构建cDNA文库的方法 |
KR102270892B1 (ko) * | 2012-07-17 | 2021-06-30 | 디엔에이 로직스, 인크. | 공조 프라이머, 프로브 그리고 이의 용도 |
US20140038182A1 (en) | 2012-07-17 | 2014-02-06 | Dna Logix, Inc. | Cooperative primers, probes, and applications thereof |
CA2900259C (en) | 2013-02-07 | 2020-12-29 | Rutgers, The State University Of New Jersey | Highly selective nucleic acid amplification primers |
GB201317355D0 (en) * | 2013-10-01 | 2013-11-13 | Epistem Ltd | Mutation Analysis |
CN103710431B (zh) * | 2013-10-30 | 2017-04-19 | 常州市第三人民医院 | 一种检测基因突变的引物及其应用 |
WO2015081229A2 (en) * | 2013-11-26 | 2015-06-04 | Lc Sciences Llc | Selective amplification of nucleic acid sequences |
US10683534B2 (en) | 2015-01-27 | 2020-06-16 | BioSpyder Technologies, Inc. | Ligation assays in liquid phase |
US9856521B2 (en) * | 2015-01-27 | 2018-01-02 | BioSpyder Technologies, Inc. | Ligation assays in liquid phase |
US20170349926A1 (en) * | 2014-12-22 | 2017-12-07 | DNAe Group Holdings LTD. | Bubble primers |
KR101742681B1 (ko) * | 2015-01-30 | 2017-06-01 | 에스디 바이오센서 주식회사 | 상보적 염기서열 내지는 미스-매치된 염기를 포함하는 상보적인 염기서열과 연결된 pcr 프라이머 및 이를 이용한 핵산 증폭 방법 |
US20180073082A1 (en) * | 2015-02-25 | 2018-03-15 | Diogene Co., Ltd | Dumbbell-structure oligonucleotide, nucleic acid amplification primer comprising same, and nucleic acid amplification method using same |
CN105154437A (zh) * | 2015-07-28 | 2015-12-16 | 中国检验检疫科学研究院 | 用于多重pcr检测转基因作物的dpo引物组合及检测方法 |
CN105274096A (zh) * | 2015-09-07 | 2016-01-27 | 中国人民解放军第三军医大学第一附属医院 | 桥式序列区掺入错配碱基的桥式荧光探针及其应用和方法 |
CN110168103B (zh) * | 2016-12-29 | 2023-09-08 | Seegene株式会社 | 降低引物二聚体形成且提高扩增效率的方法 |
KR101856205B1 (ko) | 2017-11-16 | 2018-06-20 | 제노플랜코리아 주식회사 | 핵산의 대립형질 특이적 프라이머 및 이를 이용한 유전형 판별 방법 |
KR20190056276A (ko) | 2018-05-02 | 2019-05-24 | 제노플랜코리아 주식회사 | 핵산의 대립형질 특이적 프라이머 및 이를 이용한 유전형 판별 방법 |
AU2019287163A1 (en) | 2018-06-12 | 2020-12-03 | Keygene N.V. | Nucleic acid amplification method |
CN108753935A (zh) * | 2018-06-15 | 2018-11-06 | 上海优甲医疗科技有限公司 | 一种降低多重聚合酶链式反应中引物二聚体扩增的方法 |
WO2020184551A1 (ja) * | 2019-03-13 | 2020-09-17 | 東洋紡株式会社 | 核酸の生成および増幅 |
CN109811080A (zh) * | 2019-04-02 | 2019-05-28 | 丹娜(天津)生物科技有限公司 | 一种念珠菌分种检测的dpo引物对、检测方法、试剂盒及其应用 |
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EP0425563B1 (de) | 1988-07-20 | 1996-05-15 | David Segev | Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen |
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BRPI0214741B1 (pt) * | 2001-12-08 | 2015-05-12 | Seegene Inc | Iniciador de controle de anelamento para melhorar a especificidade de anelamento na amplificação de ácido nucléico, Kit, Métodos para amplificar seqüências de ácido nucléico de um DNA e seqüência de ácido nucléico alvo ou uma mistura de ácidos nucléicos, para detectar a complementaridade de DNA ao mRNA diferencialmente expresso em duas ou mais amostras de ácido nucléico, para amplificar rapidamente um fragmento de CDNA alvo, para amplificar uma população de CDNAs de filamento duplo de comprimento completo complementar aos mRNAS, e CDNAs de filamento duplo enriquecidos em 5 complementares aos mRNAS, para amplificar mais do que uma seqüência de nucleotídeo alvo simultaneamente, para produzir uma impressão digital de DNA de gDNA, e de RNA de uma amostra de mRNA, para identificar segmentos de homologia conservada em uma família de multigene a partir de uma amostra de mRNA, para identificar uma variação de nucleotídeo em um ácido nucléico alvo, para a mutagênese em um ácido nucléico alvo, e, uso do iniciador |
AU2002339698B2 (en) | 2001-12-12 | 2007-08-23 | Serono Genetics Institute S.A. | Biallelic markers of D-amino acid oxidase and uses thereof |
AU2003240436A1 (en) * | 2002-06-20 | 2004-01-06 | Nuevolution A/S | Microarrays displaying encoded molecules |
DK1558744T3 (da) * | 2002-10-30 | 2011-10-10 | Nuevolution As | Enzymatisk kodning |
EP1597395A2 (de) * | 2003-02-21 | 2005-11-23 | Nuevolution A/S | Methode zur herstellung einer bibliothek der zweiten generation |
WO2006095941A1 (en) * | 2005-03-05 | 2006-09-14 | Seegene, Inc. | Processes using dual specificity oligonucleotide and dual specificity oligonucleotide |
FR2933490B1 (fr) | 2008-07-02 | 2010-08-27 | Commissariat Energie Atomique | Procede de mesure du debit d'un liquide en ecoulement dans un canal fluidique et dispositif de mise en oeuvre |
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2005
- 2005-04-26 WO PCT/KR2005/001206 patent/WO2006095941A1/en active Application Filing
-
2006
- 2006-03-03 ZA ZA200707514A patent/ZA200707514B/xx unknown
- 2006-03-03 EP EP11168429.6A patent/EP2365078B1/de active Active
- 2006-03-03 MX MX2007010875A patent/MX2007010875A/es active IP Right Grant
- 2006-03-03 PT PT111684296T patent/PT2365078E/pt unknown
- 2006-03-03 NZ NZ561166A patent/NZ561166A/en unknown
- 2006-03-03 DK DK11168429.6T patent/DK2365078T3/en active
- 2006-03-03 CA CA2599647A patent/CA2599647C/en active Active
- 2006-03-03 ES ES11168429.6T patent/ES2447297T3/es active Active
- 2006-03-03 AU AU2006221202A patent/AU2006221202B2/en active Active
- 2006-03-03 KR KR1020147010516A patent/KR101534228B1/ko active IP Right Grant
- 2006-03-03 KR KR1020137001209A patent/KR20130018374A/ko not_active Application Discontinuation
- 2006-03-03 DK DK06716196.8T patent/DK1856257T3/da active
- 2006-03-03 PL PL11168429T patent/PL2365078T3/pl unknown
- 2006-03-03 CN CN2006800072179A patent/CN101189336B/zh active Active
- 2006-03-03 JP JP2007557937A patent/JP4903725B2/ja active Active
- 2006-03-03 US US11/817,838 patent/US8092997B2/en active Active
- 2006-03-03 KR KR1020137023486A patent/KR101440404B1/ko active IP Right Grant
- 2006-03-03 ES ES06716196T patent/ES2375571T3/es active Active
- 2006-03-03 AT AT06716196T patent/ATE529534T1/de active
- 2006-03-03 BR BRPI0609031-1A patent/BRPI0609031B1/pt active IP Right Grant
- 2006-06-22 MY MYPI20062962A patent/MY162156A/en unknown
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2007
- 2007-08-29 NO NO20074394A patent/NO339577B1/no unknown
- 2007-09-03 IL IL185683A patent/IL185683A/en active IP Right Grant
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2011
- 2011-12-19 US US13/329,667 patent/US8323895B2/en active Active
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2012
- 2012-11-08 US US13/671,621 patent/US9884890B2/en active Active
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2016
- 2016-07-08 NO NO20161140A patent/NO340891B1/no unknown
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2018
- 2018-01-09 US US15/865,554 patent/US10870675B2/en active Active
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