ATE510932T1 - Mrna-quantifizierung einer einzelzelle mittels echtzeit-rt-pcr - Google Patents

Mrna-quantifizierung einer einzelzelle mittels echtzeit-rt-pcr

Info

Publication number
ATE510932T1
ATE510932T1 AT08749213T AT08749213T ATE510932T1 AT E510932 T1 ATE510932 T1 AT E510932T1 AT 08749213 T AT08749213 T AT 08749213T AT 08749213 T AT08749213 T AT 08749213T AT E510932 T1 ATE510932 T1 AT E510932T1
Authority
AT
Austria
Prior art keywords
pcr
sample
primers
real
time
Prior art date
Application number
AT08749213T
Other languages
English (en)
Inventor
Martin Bengtsson
Michael Kubista
Anders Stahlberg
Linda Stroembom
Original Assignee
Hoffmann La Roche
Tataa Bioct Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche, Tataa Bioct Ab filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE510932T1 publication Critical patent/ATE510932T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT08749213T 2007-05-03 2008-04-29 Mrna-quantifizierung einer einzelzelle mittels echtzeit-rt-pcr ATE510932T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07008961 2007-05-03
PCT/EP2008/003451 WO2008135196A1 (en) 2007-05-03 2008-04-29 SINGLE-CELL mRNA QUANTIFICATION WITH REAL-TIME RT-PCR

Publications (1)

Publication Number Publication Date
ATE510932T1 true ATE510932T1 (de) 2011-06-15

Family

ID=38050036

Family Applications (1)

Application Number Title Priority Date Filing Date
AT08749213T ATE510932T1 (de) 2007-05-03 2008-04-29 Mrna-quantifizierung einer einzelzelle mittels echtzeit-rt-pcr

Country Status (5)

Country Link
US (3) US8642266B2 (de)
EP (2) EP2147120B1 (de)
AT (1) ATE510932T1 (de)
ES (2) ES2472765T3 (de)
WO (2) WO2008135196A1 (de)

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WO2008135196A1 (en) * 2007-05-03 2008-11-13 Roche Diagnostics Gmbh SINGLE-CELL mRNA QUANTIFICATION WITH REAL-TIME RT-PCR
CA2730761C (en) * 2008-08-01 2016-04-19 F. Hoffmann-La Roche Ag Improved lysis and reverse transcription for mrna quantification
RU2536291C2 (ru) 2008-12-04 2014-12-20 Массачусетс Инститьют Оф Текнолоджи Способ диагностики аллергических реакций
US9404924B2 (en) * 2008-12-04 2016-08-02 Massachusetts Institute Of Technology Method of performing one-step, single cell RT-PCR
JPWO2010131645A1 (ja) * 2009-05-14 2012-11-01 和光純薬工業株式会社 Ｒｎａに対応する二本鎖ｄｎａの合成方法及び増幅方法
US9486803B2 (en) 2010-01-22 2016-11-08 Biotix, Inc. Pipette tips
WO2011104032A1 (en) * 2010-02-26 2011-09-01 Qiagen Gmbh Method for isolating rna from a rna and dna containing sample
KR101420094B1 (ko) 2010-10-27 2014-07-17 (주)바이오니아 다양한 생체시료분석을 위한 전자동실시간정량증폭장비, 다양한 생체시료분석을 위한 자동정제 및 반응준비 장치, 전자동 핵산정제 및 실시간 정량 유전자증폭 방법, 전자동 핵산정제방법, 실시간정량ｐｃｒ을 이용한 병원균의 전자동 생균수검사방법, 정량면역ｐｃｒ을 이용한 전자동 항원농도획득방법 및 타겟항원에 라벨링된 부착용 타겟핵산의 정제방법
US8795606B2 (en) 2012-05-30 2014-08-05 Biotix, Inc. Integrated pipette tip devices
JP6317353B2 (ja) 2012-09-13 2018-04-25 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 固形組織の単細胞における多重分析測定のための方法
JP2015532114A (ja) * 2012-10-20 2015-11-09 セルフダイアグノスティックス オーウー 生物学的サンプル及び体液に由来する核酸標的の検出のための方法及びその組成物
GB2559919B (en) 2014-02-28 2018-11-14 Gen Probe Inc Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde
WO2016077750A1 (en) 2014-11-14 2016-05-19 Athena Diagnostics, Inc. Methods to detect a silent carrier genotype
JP2017209036A (ja) * 2016-05-24 2017-11-30 東洋紡株式会社 改良されたウイルス検出方法
CN110944751B (zh) 2017-05-17 2022-04-01 拜欧迪克斯公司 人体工学移液管吸头
GB201803654D0 (en) 2018-03-07 2018-04-25 Arcticzymes As Thermolabile proteinases
CN113557298A (zh) * 2019-03-13 2021-10-26 东洋纺株式会社 核酸的生成和扩增
WO2021221109A1 (ja) * 2020-04-30 2021-11-04 タカラバイオ株式会社 Ｒｎａウイルスの検出方法

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US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
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Also Published As

Publication number Publication date
EP2147120B1 (de) 2014-04-02
EP2147120A1 (de) 2010-01-27
US20110136180A1 (en) 2011-06-09
ES2472765T3 (es) 2014-07-03
US8642266B2 (en) 2014-02-04
WO2008135196A1 (en) 2008-11-13
EP2147119A1 (de) 2010-01-27
ES2365447T8 (es) 2011-11-21
US20100216194A1 (en) 2010-08-26
EP2147119B1 (de) 2011-05-25
US20160168631A1 (en) 2016-06-16
ES2365447T3 (es) 2011-10-05
US9200313B2 (en) 2015-12-01
WO2008135197A1 (en) 2008-11-13

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