ATE452982T1 - Verfahren zur verabreichng von nukleinsäuren in zellen in vitro oder ex vivo - Google Patents

Verfahren zur verabreichng von nukleinsäuren in zellen in vitro oder ex vivo

Info

Publication number
ATE452982T1
ATE452982T1 AT99401687T AT99401687T ATE452982T1 AT E452982 T1 ATE452982 T1 AT E452982T1 AT 99401687 T AT99401687 T AT 99401687T AT 99401687 T AT99401687 T AT 99401687T AT E452982 T1 ATE452982 T1 AT E452982T1
Authority
AT
Austria
Prior art keywords
cells
vitro
vivo
administration
nucleic acids
Prior art date
Application number
AT99401687T
Other languages
English (en)
Inventor
Francois Lemoine
David Klatzmann
Mojgan Movassagh
Jean-Loup Salzmann
Original Assignee
Univ Paris Curie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Paris Curie filed Critical Univ Paris Curie
Application granted granted Critical
Publication of ATE452982T1 publication Critical patent/ATE452982T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13045—Special targeting system for viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13051—Methods of production or purification of viral material
    • C12N2740/13052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
    • C12N2810/6045—RNA rev transcr viruses
    • C12N2810/6054—Retroviridae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Veterinary Medicine (AREA)
  • Plant Pathology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT99401687T 1998-07-10 1999-07-06 Verfahren zur verabreichng von nukleinsäuren in zellen in vitro oder ex vivo ATE452982T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US11328098A 1998-07-10 1998-07-10

Publications (1)

Publication Number Publication Date
ATE452982T1 true ATE452982T1 (de) 2010-01-15

Family

ID=22348556

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99401687T ATE452982T1 (de) 1998-07-10 1999-07-06 Verfahren zur verabreichng von nukleinsäuren in zellen in vitro oder ex vivo

Country Status (4)

Country Link
EP (1) EP0995802B1 (de)
JP (1) JP2000139478A (de)
AT (1) ATE452982T1 (de)
DE (1) DE69941836D1 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9493789B2 (en) * 2014-04-24 2016-11-15 Miltenyi Biotec Gmbh Automated generation of genetically modified stem cells
TWI735418B (zh) * 2014-11-05 2021-08-11 美商奇諾治療有限公司 用於轉導作用及細胞處理之方法
EP3858365B1 (de) * 2016-09-01 2024-01-31 Chimera Bioengineering Inc. Goldoptimierte car-t-zellen

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5017492A (en) * 1986-02-27 1991-05-21 Life Technologies, Inc. Reverse transcriptase and method for its production
EP0857217B1 (de) * 1995-10-16 2005-04-13 Dana-Farber Cancer Institute Neue expressionsvektoren und verfahren zu deren verwendung
AU2998597A (en) * 1996-05-06 1997-11-26 Chiron Corporation Crossless retroviral vectors
AU4012900A (en) * 1999-03-15 2000-10-04 Chiron Corporation Methods for producing a vector producing cell line utilizing a high multiplicityof transduction

Also Published As

Publication number Publication date
JP2000139478A (ja) 2000-05-23
DE69941836D1 (de) 2010-02-04
EP0995802A3 (de) 2001-01-10
EP0995802B1 (de) 2009-12-23
EP0995802A2 (de) 2000-04-26

Similar Documents

Publication Publication Date Title
Baramova et al. Degradation of extracellular matrix proteins by hemorrhagic metalloproteinases
Higuchi et al. DNA sequences from the quagga, an extinct member of the horse family
ATE318896T1 (de) Rekombinante rna-replikase von hepatitis-c-virus
DE69906977D1 (de) In liposomen verkapselte nukleinsäurekomplexe
DE59912118D1 (de) Verfahren zur herstellung von genetisch modifizierten cd34-negativen, adhärent wachsenden hämatopoietischen stammzellen
ATE87663T1 (de) Verfahren zur herstellung von faktor-viii:c-typ- proteinen.
DE3752319T2 (de) Prozess zur rekombinanten Herstellung von HIV-2 - Proteinen sowie Zellen, die HIV-2 - Proteine exprimiert
EP0161640A3 (de) Mit "Vogelretrovirus-Rinderwachstumshormon"-DNS transformierte eingekapselte Mausezellen und Verfahren zum Verabreichen von BGH in vivo
DE69720026T2 (de) Verfahren zur herstellung von d-aminosäuren durch direkte gärung
ATE278024T1 (de) Methode zur herstellung von rekombinanten zellen
DE69131069T2 (de) Plasmid das für die aminosäuresequenz von tcf-ii kodierende dns enthalt, transformierte zelle und produktion einer physiologisch aktiven substanz mit ihrer verwendung
ATE339511T1 (de) Natürliche oder synthetische retroelement- sequenzen, die die insertion von nukleotiden in eukaryotische zellen erlauben
CO4410255A1 (es) Procedimiento para introducir acido nucleico en celulas eucarioticas superiores y medio para transfeccion
KR880009991A (ko) 항혈액응고작용을 갖는 폴리펩티드
ATE452982T1 (de) Verfahren zur verabreichng von nukleinsäuren in zellen in vitro oder ex vivo
DE68929296D1 (de) Verfahren zur biochemischen Oxidation von Steroiden sowie dafür verwendbare, genetisch manipulierte Zellen
ATE233321T1 (de) Verbesserungen in der herstellung von proteinen in wirtszellen
DE69425809T2 (de) Methoden zur Herstellung von Protein C
ES2061524T3 (es) Un vector plasmidico para la expresion en bacillus y empleado para la clonacion del gen estructural que codifica la hormona de crecimiento humana y un metodo para la produccion de la hormona.
ES2053766T3 (es) Metodo para aumentar la expresion de un gen.
DE60030621D1 (de) DNA-Konstrukt zur gewebespezifischen Expression eines Blutkgerinnungsfaktors
ATE341630T1 (de) Verwendung von pankreatischer procarboxypeptidase b zur herstellung von insulin
ATE302282T1 (de) Erhöhte herstellung von sekretierten proteinen durch rekombinante hefezellen
EA200101093A1 (ru) Экспрессия гепарин-связывающего протеина в рекомбинантных клетках млекопитающих
WO1998053063A3 (en) Mammalian cell transduction for use in gene therapy

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties