ATE446511T1 - Neue fusionsproteine und molekulare bindungsassays - Google Patents

Neue fusionsproteine und molekulare bindungsassays

Info

Publication number
ATE446511T1
ATE446511T1 AT02773169T AT02773169T ATE446511T1 AT E446511 T1 ATE446511 T1 AT E446511T1 AT 02773169 T AT02773169 T AT 02773169T AT 02773169 T AT02773169 T AT 02773169T AT E446511 T1 ATE446511 T1 AT E446511T1
Authority
AT
Austria
Prior art keywords
fusion proteins
expression vectors
binding assay
binding
molecular binding
Prior art date
Application number
AT02773169T
Other languages
English (en)
Inventor
Gary Bright
Daniel Premkumar
Yih-Tai Chen
Original Assignee
Cellomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellomics Inc filed Critical Cellomics Inc
Application granted granted Critical
Publication of ATE446511T1 publication Critical patent/ATE446511T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/095—Fusion polypeptide containing a localisation/targetting motif containing a nuclear export signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/41—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a Myc-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT02773169T 2001-08-01 2002-08-01 Neue fusionsproteine und molekulare bindungsassays ATE446511T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US30939501P 2001-08-01 2001-08-01
US34158901P 2001-12-13 2001-12-13
PCT/US2002/024572 WO2003012068A2 (en) 2001-08-01 2002-08-01 Novel fusion proteins and assays for molecular binding

Publications (1)

Publication Number Publication Date
ATE446511T1 true ATE446511T1 (de) 2009-11-15

Family

ID=26976793

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02773169T ATE446511T1 (de) 2001-08-01 2002-08-01 Neue fusionsproteine und molekulare bindungsassays

Country Status (8)

Country Link
US (1) US7244614B2 (de)
EP (1) EP1499885B1 (de)
JP (1) JP2005507650A (de)
AT (1) ATE446511T1 (de)
AU (1) AU2002337664B2 (de)
CA (1) CA2453528C (de)
DE (1) DE60234131D1 (de)
WO (1) WO2003012068A2 (de)

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US7575939B2 (en) 2000-10-30 2009-08-18 Sru Biosystems, Inc. Optical detection of label-free biomolecular interactions using microreplicated plastic sensor elements
US7371562B2 (en) * 2000-10-30 2008-05-13 Sru Biosystems, Inc. Guided mode resonant filter biosensor using a linear grating surface structure
US7524625B2 (en) * 2000-10-30 2009-04-28 Sru Biosystems, Inc. Real time binding analysis of antigens on a biosensor surface
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DE60234131D1 (de) * 2001-08-01 2009-12-03 Cellomics Inc Neue fusionsproteine und molekulare bindungsassays
US7927822B2 (en) 2002-09-09 2011-04-19 Sru Biosystems, Inc. Methods for screening cells and antibodies
US7309614B1 (en) 2002-12-04 2007-12-18 Sru Biosystems, Inc. Self-referencing biodetection method and patterned bioassays
JP4374953B2 (ja) * 2003-09-09 2009-12-02 株式会社日立製作所 データ処理システム
US8298780B2 (en) * 2003-09-22 2012-10-30 X-Body, Inc. Methods of detection of changes in cells
JP2008507995A (ja) * 2004-08-02 2008-03-21 セルーメン、インコーポレイテッド 細胞内での分子相互作用の検出方法
DK1988167T3 (da) * 2005-02-17 2020-07-13 Univ Sorbonne Intracellulære inhiberende peptider
NZ562794A (en) * 2005-04-12 2010-07-30 Sru Biosystems Inc Proteolipid membrane & lipid membrane biosensor using a titanium surface
WO2007069817A1 (en) * 2005-12-16 2007-06-21 National Cancer Center Peptides for inhibiting transglutaminase
GB0526449D0 (en) * 2005-12-23 2006-02-08 Medical Res Council Polypeptide targeting
WO2008018905A2 (en) * 2006-01-17 2008-02-14 Cellumen, Inc. Method for predicting biological systems responses
US8341123B2 (en) 2006-01-27 2012-12-25 El Fresko Technologies Limited Event structured file system (ESFS)
US20070203325A1 (en) * 2006-02-24 2007-08-30 Xiaojian Yao Integrase-dirived HIV-inhibiting agents
JPWO2007132555A1 (ja) * 2006-05-11 2009-09-24 国立大学法人 東京大学 細胞膜透過性ペプチドと細胞内におけるその使用
JP5406019B2 (ja) 2006-05-17 2014-02-05 セルーメン、インコーポレイテッド 自動化組織分析のための方法
EP2095119A2 (de) * 2006-11-10 2009-09-02 Cellumen, Inc. Biosensoren für protein-protein-interaktion und verfahren zu ihrer verwendung
WO2008115420A2 (en) * 2007-03-15 2008-09-25 Cellumen, Inc. Methods for detecting molecular interactions within cells using combination of inducible promoters and biosensors
US9134307B2 (en) 2007-07-11 2015-09-15 X-Body, Inc. Method for determining ion channel modulating properties of a test reagent
NZ582473A (en) 2007-07-11 2011-12-22 Sru Biosystems Inc Methods of identifying modulators of ion channels using a colorimetric resonant reflectance optical biosensor
US8257936B2 (en) 2008-04-09 2012-09-04 X-Body Inc. High resolution label free analysis of cellular properties
US20100273185A1 (en) * 2009-04-27 2010-10-28 Sru Biosystems, Inc. Detection of Biased Agonist Activation
US20130266663A1 (en) * 2010-04-30 2013-10-10 Arthur Brown Sox9 inhibitors
EP2686688B1 (de) 2011-03-17 2019-05-08 Cernostics, Inc. Systeme und zusammensetzungen zur diagnose von barrett-ösophagen und verwendungsverfahren dafür
EP2870475B1 (de) * 2012-07-06 2017-06-07 Innovative Technologies in Biological Systems S.L. Fluoreszentes fusionspolypeptid, biosensor mit besagtem polypeptid und verwendungen davon
CN109608550B (zh) * 2012-12-05 2020-09-11 生控基因疫苗股份有限公司 用作诱发抗原特异性t细胞反应的免疫原性增强剂的融合蛋白
CA2906124A1 (en) 2013-03-15 2014-09-18 Bayer Healthcare Llc Substrate profiling of proteases in neutrophil extracellular traps
US10591486B2 (en) 2014-07-23 2020-03-17 Institut National De La Sante Et De La Recherche Medicale (Inserm) Methods and tools for detecting interactions in eukaryotic cells using microtubule structures and dynamics
EP3272864A1 (de) 2016-07-20 2018-01-24 Paul Scherrer Institut Festphasenimmobilisierung von mikrobieller transglutaminase-mtg auf mikrokugeln für proteinkonjugation
KR20200057046A (ko) * 2017-09-19 2020-05-25 폴 슈레 앙스띠뛰 트랜스글루타미나제 컨쥬게이션 방법 및 링커
BR112022006923A2 (pt) * 2019-10-11 2022-07-05 Univ Leland Stanford Junior Polipeptídeos recombinantes para localização celular regulável
WO2024050383A2 (en) * 2022-08-29 2024-03-07 The Regents Of The University Of California Ras biosensors

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DE60234131D1 (de) * 2001-08-01 2009-12-03 Cellomics Inc Neue fusionsproteine und molekulare bindungsassays

Also Published As

Publication number Publication date
EP1499885A2 (de) 2005-01-26
US20030104479A1 (en) 2003-06-05
US7244614B2 (en) 2007-07-17
EP1499885B1 (de) 2009-10-21
CA2453528A1 (en) 2003-02-13
AU2002337664B2 (en) 2007-01-25
DE60234131D1 (de) 2009-12-03
WO2003012068A2 (en) 2003-02-13
JP2005507650A (ja) 2005-03-24
EP1499885A4 (de) 2005-11-16
WO2003012068A3 (en) 2004-11-11
CA2453528C (en) 2011-07-26

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