ATE443136T1 - Methode für dna synthese - Google Patents

Methode für dna synthese

Info

Publication number
ATE443136T1
ATE443136T1 AT07104945T AT07104945T ATE443136T1 AT E443136 T1 ATE443136 T1 AT E443136T1 AT 07104945 T AT07104945 T AT 07104945T AT 07104945 T AT07104945 T AT 07104945T AT E443136 T1 ATE443136 T1 AT E443136T1
Authority
AT
Austria
Prior art keywords
dna synthesis
dna
synthesis reaction
enhancer
reaction
Prior art date
Application number
AT07104945T
Other languages
English (en)
Inventor
Kiyozo Asada
Yoshimi Sato
Takashi Uemori
Tomoko Fujita
Kazue Miyake
Osamu Takeda
Hiroyuki Mukai
Ikunoshin Kato
Original Assignee
Takara Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Bio Inc filed Critical Takara Bio Inc
Application granted granted Critical
Publication of ATE443136T1 publication Critical patent/ATE443136T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
AT07104945T 1998-04-23 1999-04-21 Methode für dna synthese ATE443136T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP11400598 1998-04-23
JP31524398 1998-11-06

Publications (1)

Publication Number Publication Date
ATE443136T1 true ATE443136T1 (de) 2009-10-15

Family

ID=26452869

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07104945T ATE443136T1 (de) 1998-04-23 1999-04-21 Methode für dna synthese

Country Status (10)

Country Link
US (2) US7521178B1 (de)
EP (3) EP1816197B1 (de)
JP (2) JP3748378B2 (de)
KR (1) KR100519895B1 (de)
CN (2) CN1177931C (de)
AT (1) ATE443136T1 (de)
AU (1) AU3534199A (de)
CA (1) CA2326725A1 (de)
DE (1) DE69941441D1 (de)
WO (1) WO1999054455A1 (de)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3724321B2 (ja) * 2000-03-21 2005-12-07 株式会社島津製作所 核酸合成法
TW201037080A (en) 2003-12-25 2010-10-16 Danform Kk Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
US7462475B2 (en) * 2004-05-20 2008-12-09 Dna Poleymerase Technology, Inc. Use of whole blood in PCR reactions
JP2008151771A (ja) 2006-11-22 2008-07-03 Fujifilm Corp マイクロ流体チップ
CA2622649C (en) 2007-03-09 2018-04-24 Riken Nucleic acid amplification method using primer exhibiting exciton effect
US20100221787A1 (en) 2007-10-25 2010-09-02 Riken Isothermal amplification method and dna polymerase used in the same
EP2256188B1 (de) * 2008-02-29 2014-06-18 Riken Verfahren zur erhöhung der enzymatischen reaktivität
WO2010061922A1 (ja) 2008-11-27 2010-06-03 独立行政法人理化学研究所 新規ＭｕｔＳタンパク質およびそれを用いた変異の判定方法
WO2010080910A1 (en) * 2009-01-08 2010-07-15 Bio-Rad Laboratories, Inc. Methods and compositions for improving efficiency of nucleic acids amplification reactions
EP3502272A1 (de) * 2010-06-21 2019-06-26 Life Technologies Corporation Zusammensetzungen, kits und verfahren zur synthese und/oder zum nachweis von nukleinsäuren
EP2600901B1 (de) 2010-08-06 2019-03-27 ModernaTX, Inc. Pharmazeutische zusammensetzungen enthaltenbearbeitete nukleinsäuren und ihre medizinische verwendung
CA2821992A1 (en) 2010-10-01 2012-04-05 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
US9458498B2 (en) 2011-01-24 2016-10-04 Takara Bio Inc. Method for modifying nucleic acids
JP2014511687A (ja) 2011-03-31 2014-05-19 モデルナ セラピューティクス インコーポレイテッド 工学操作された核酸の送達および製剤
US9586987B2 (en) 2011-09-08 2017-03-07 Kabushiki Kaisha Dnaform Primer set for isothermal amplication of a target nucleic acid sequence
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
US9428535B2 (en) 2011-10-03 2016-08-30 Moderna Therapeutics, Inc. Modified nucleosides, nucleotides, and nucleic acids, and uses thereof
HRP20220717T1 (hr) 2011-12-16 2022-07-22 Modernatx, Inc. Modificirani pripravci mrna
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
JP2015513912A (ja) 2012-04-02 2015-05-18 モデルナ セラピューティクス インコーポレイテッドＭｏｄｅｒｎａ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． タンパク質の産生のための修飾ポリヌクレオチド
US9254311B2 (en) 2012-04-02 2016-02-09 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins
CN104812901B (zh) 2012-09-13 2018-08-10 美国印第安纳大学研究和技术公司 赋予植物抗病性的组合物和***及其使用方法
EP2922554B1 (de) 2012-11-26 2022-02-23 ModernaTX, Inc. Terminal modifizierte rns
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
US20160194625A1 (en) 2013-09-03 2016-07-07 Moderna Therapeutics, Inc. Chimeric polynucleotides
AU2014329452B2 (en) 2013-10-03 2019-06-20 Moderna Therapeutics, Inc. Polynucleotides encoding low density lipoprotein receptor
US20150259743A1 (en) 2013-12-31 2015-09-17 Roche Nimblegen, Inc. Methods of assessing epigenetic regulation of genome function via dna methylation status and systems and kits therefor
WO2015114469A2 (en) 2014-01-15 2015-08-06 Abbott Japan Co., Ltd Covered sequence conversion dna and detection methods
WO2016011226A1 (en) 2014-07-16 2016-01-21 Moderna Therapeutics, Inc. Chimeric polynucleotides
CN108064312B (zh) 2014-12-24 2022-04-08 雅培实验室 基于具有靶扩增的信号放大dna级联反应的检测方法
KR101785074B1 (ko) 2015-01-28 2017-10-12 고려대학교 산학협력단 Dna 합성용 마이크로 칩 및 이를 이용한 dna 합성방법
SI3394093T1 (sl) 2015-12-23 2022-05-31 Modernatx, Inc. Metode uporabe liganda OX40, ki kodira polinukleotid
US20190241658A1 (en) 2016-01-10 2019-08-08 Modernatx, Inc. Therapeutic mRNAs encoding anti CTLA-4 antibodies
AU2017268397B2 (en) 2016-05-18 2023-07-06 Modernatx, Inc. Polynucleotides encoding interleukin-12 (IL12) and uses thereof
WO2017201325A1 (en) 2016-05-18 2017-11-23 Modernatx, Inc. Combinations of mrnas encoding immune modulating polypeptides and uses thereof
CN106916909B (zh) * 2017-05-09 2018-09-11 广州海力特生物科技有限公司 一种HBV pgRNA的实时荧光定量RT-PCR检测引物组、探针组、试剂盒及方法
WO2018231990A2 (en) 2017-06-14 2018-12-20 Modernatx, Inc. Polynucleotides encoding methylmalonyl-coa mutase
US11905518B2 (en) 2018-02-12 2024-02-20 Curators Of The University Of Missouri Small auxin upregulated (SAUR) gene for the improvement of root system architecture, waterlogging tolerance, drought resistance and yield in plants and methods of uses
WO2020185312A2 (en) 2019-01-25 2020-09-17 President And Fellows Of Harvard College Compositions and method for synthesizing nucleic acids
JP2023506176A (ja) 2019-12-12 2023-02-15 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 光誘導型生体分子バーコーディングのための組成物および方法
CA3176545A1 (en) 2020-04-22 2021-10-28 President And Fellows Of Harvard College Isothermal methods, compositions, kits, and systems for detecting nucleic acids

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4072574A (en) * 1976-07-30 1978-02-07 The Institute For Cancer Research System for in vitro assay for mutagenicity and/or carcinogenicity of chemicals or other exogenous agents
US5225556A (en) * 1985-01-18 1993-07-06 The Trustees Of Columbia University In The City Of New York Chemical probes for left-handed DNA and for A-DNA; chiral metal complexes as Z-specific antitumor agents and as double strand cleavers
DE3601136A1 (de) * 1986-01-16 1987-07-23 Max Planck Gesellschaft Hemmstoffe der reversen transkriptase fuer prophylaxe und therapie von retrovirus-infektionen in saeugetieren
CA2065719A1 (en) 1991-04-30 1992-10-31 John B. Findlay Nucleic acid amplification and detection methods using rapid polymerase chain reaction cycle
DE69231178T2 (de) * 1991-08-30 2000-11-23 Syngenix Ltd., Cambridge Nukleinsäure-amplifikation durch polymerase-reaktion
US5418162A (en) * 1991-10-22 1995-05-23 Duke University Serotonin transporter CDNA
AU4248593A (en) * 1992-05-14 1993-12-13 Mark Holodniy Polymerase chain reaction assays for monitoring antiviral therapy
DK0590327T3 (da) * 1992-09-11 2003-07-28 Hoffmann La Roche Påvisning af nukleinsyrer i blod
RU2048522C1 (ru) * 1992-10-14 1995-11-20 Институт белка РАН Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления
US6410277B1 (en) * 1993-02-19 2002-06-25 Takara Shuzo Co., Ltd. DNA polymersases with enhanced length of primer extension
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5556772A (en) * 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
US6087097A (en) * 1994-05-12 2000-07-11 Mayo Foundation For Medical Education And Research PCR detection of Borrelia burgdorferi
EP0745687A1 (de) 1995-04-08 1996-12-04 Roche Diagnostics GmbH Verfahren zur spezifischen Vervielfältigung und zum Nachweis von DNA bzw. RNA
US5789206A (en) * 1995-07-07 1998-08-04 Myriad Genetics, Inc. Method for ligating adaptors to nucleic acids which methods are useful for obtaining the ends of genes
US5795722A (en) * 1997-03-18 1998-08-18 Visible Genetics Inc. Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
CN1137266C (zh) 1995-12-27 2004-02-04 宝酒造株式会社 新型dna聚合酶
CA2243543A1 (en) * 1996-01-26 1997-07-31 Research Institute For Glycotechnology Apoptosis inducers
CA2248981C (en) * 1996-03-15 2009-11-24 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
US5917031A (en) * 1996-04-19 1999-06-29 Taiko Pharmaceutical Co., Ltd. Method of synthesizing polydeoxyribonucleotides
US5928906A (en) * 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
DE69736110T2 (de) 1996-06-12 2007-01-18 Takara Bio Inc., Otsu Verwendung von Fucoidin
JP3463780B2 (ja) 1996-07-30 2003-11-05 東洋紡績株式会社 核酸増幅用ｄｎａポリメラーゼ組成物
DE69725076T2 (de) 1996-07-29 2004-04-15 Toyo Boseki K.K. Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
EP1705253B1 (de) * 1997-04-03 2010-11-03 Life Technologies Corporation Verfahren und Produkte zur reversen Transkription gekoppelt mit Polymerasekettenreaktion (RT-PCR)
US5939259A (en) * 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis
US6348336B1 (en) * 1997-07-01 2002-02-19 Alpha Therapeutic Corporation Process for purification of PCR test samples
KR100559098B1 (ko) * 1998-09-08 2006-03-15 다카라 바이오 가부시키가이샤 Ｄｎａ 의 합성방법
EP0989192A3 (de) 1998-09-21 2004-02-25 Shimadzu Corporation Verfahren zur Nucleinsäuresynthese
JP2001029078A (ja) 1999-07-16 2001-02-06 Shimadzu Corp Ｒｎａ増幅法
GB0001450D0 (en) * 2000-01-21 2000-03-08 Genpoint As Cell isolation method
DE10006662A1 (de) * 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
US6667165B2 (en) * 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases

Also Published As

Publication number Publication date
CA2326725A1 (en) 1999-10-28
WO1999054455A1 (en) 1999-10-28
EP1072678A4 (de) 2006-09-27
EP1072678A1 (de) 2001-01-31
EP1816197A3 (de) 2007-11-28
KR20010042958A (ko) 2001-05-25
EP2116600B1 (de) 2013-09-18
EP1816197B1 (de) 2009-09-16
EP2116600A3 (de) 2010-01-13
JP3748378B2 (ja) 2006-02-22
CN1306570A (zh) 2001-08-01
EP2116600A2 (de) 2009-11-11
CN1637146A (zh) 2005-07-13
US8367328B2 (en) 2013-02-05
JP4214142B2 (ja) 2009-01-28
AU3534199A (en) 1999-11-08
US20090098613A1 (en) 2009-04-16
JP2006061164A (ja) 2006-03-09
DE69941441D1 (de) 2009-10-29
CN1177931C (zh) 2004-12-01
KR100519895B1 (ko) 2005-10-13
EP1816197A2 (de) 2007-08-08
US7521178B1 (en) 2009-04-21

Similar Documents

Publication Publication Date Title
ATE443136T1 (de) Methode für dna synthese
EP2272952B8 (de) Thermostabiler oder thermoactiver DNA-polymerase mit abgeschwächter 3'-5' Exonukleaseaktivität
CA2310854A1 (en) Isolation and identification of novel polymerases
EP1602725A3 (de) Enzymatische Nukleinsäure die Abasische Nukleotide enthaltet
ATE373672T1 (de) Synthese von methoxynukleoside und enzymatische nukleisäure moleküle
PT669401E (pt) Amplificacao de sequencias compridas de acidos nucleicos
ATE374833T1 (de) Verfahren zur synthese von nukleinsäuren
ATE244259T1 (de) Verwendungen von fluoreszierenden n-nukleosiden und dessen analogen
WO2001038584A3 (en) Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof
ATE427992T1 (de) Dna-synthesepromotoren, mit dna-polymerase assoziierte faktoren und nutzung davon
ATE324464T1 (de) Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
BR9407712A (pt) Métodos e composiçoes para sequenciamento eficaz de ácidos nuclêicos
DE69435232D1 (de) DNS Polymerasen
FI960540A (fi) Eksonukleaasin ja/tai glykosylaasin käyttö antipolymeraasivasta-aineenlisäaineina spesifisyyden lisäämiseksi polymeraasiketjureaktiossa
ATE317023T1 (de) Methode zum nachweis von variationen oder polymorphismen
EP0822256A3 (de) Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
CA2295306A1 (en) Dna polymerase-related factors
DE69734604D1 (de) Synthetische polynukleotide
DK0625194T3 (da) Forøgelse af ribozym-katalytisk aktivitet med et tilstødende facilitator oligonukleotid
ATE307199T1 (de) Verfahren und zusammensetzungen zur isolierung und reinigung von nukleinsäuren
WO1998009951A3 (en) Intermediates for making hiv-protease inhibitors and methods of making hiv-protease inhibitors

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties