ATE420061T1 - 13c,15n,2h markierte proteine zur nmr strukturbestimmung und deren herstellung - Google Patents
13c,15n,2h markierte proteine zur nmr strukturbestimmung und deren herstellungInfo
- Publication number
- ATE420061T1 ATE420061T1 AT98945835T AT98945835T ATE420061T1 AT E420061 T1 ATE420061 T1 AT E420061T1 AT 98945835 T AT98945835 T AT 98945835T AT 98945835 T AT98945835 T AT 98945835T AT E420061 T1 ATE420061 T1 AT E420061T1
- Authority
- AT
- Austria
- Prior art keywords
- labeled proteins
- production
- methods
- labeled
- proteins
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 5
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 238000000034 method Methods 0.000 abstract 3
- 150000001413 amino acids Chemical class 0.000 abstract 2
- 229920002521 macromolecule Polymers 0.000 abstract 1
- 238000009629 microbiological culture Methods 0.000 abstract 1
- 238000000655 nuclear magnetic resonance spectrum Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B59/00—Introduction of isotopes of elements into organic compounds ; Labelled organic compounds per se
- C07B59/008—Peptides; Proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
- G01R33/44—Arrangements or instruments for measuring magnetic variables involving magnetic resonance using nuclear magnetic resonance [NMR]
- G01R33/46—NMR spectroscopy
- G01R33/465—NMR spectroscopy applied to biological material, e.g. in vitro testing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- High Energy & Nuclear Physics (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Reproductive Health (AREA)
- Virology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/921,554 US6111066A (en) | 1997-09-02 | 1997-09-02 | Peptidic molecules which have been isotopically substituted with 13 C, 15 N and 2 H in the backbone but not in the sidechains |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE420061T1 true ATE420061T1 (de) | 2009-01-15 |
Family
ID=25445613
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT98945835T ATE420061T1 (de) | 1997-09-02 | 1998-09-02 | 13c,15n,2h markierte proteine zur nmr strukturbestimmung und deren herstellung |
Country Status (12)
Country | Link |
---|---|
US (4) | US6111066A (de) |
EP (2) | EP1889823A1 (de) |
JP (1) | JP2001514239A (de) |
AT (1) | ATE420061T1 (de) |
AU (1) | AU754232B2 (de) |
CA (1) | CA2301046C (de) |
DE (1) | DE69840448D1 (de) |
HU (1) | HUP0003383A3 (de) |
NO (1) | NO20001052L (de) |
TW (1) | TW581772B (de) |
WO (1) | WO1999011589A1 (de) |
ZA (1) | ZA987975B (de) |
Families Citing this family (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6111066A (en) | 1997-09-02 | 2000-08-29 | Martek Biosciences Corporation | Peptidic molecules which have been isotopically substituted with 13 C, 15 N and 2 H in the backbone but not in the sidechains |
US6882939B2 (en) * | 2000-10-20 | 2005-04-19 | Prospect Pharma | Rapid determination of protein global folds |
EP1328815A1 (de) * | 2000-10-20 | 2003-07-23 | Martek Biosciences Corporation | Schnelle bestimmung der global folds von proteinen |
AU2002332390A1 (en) * | 2001-03-12 | 2003-03-03 | Affinium Pharmaceuticals, Inc. | Proteins, druggable regions of proteins and target analysis for chemistry of therapeutics |
WO2003006488A1 (en) * | 2001-07-13 | 2003-01-23 | The Regents Of The University Of California | In-cell nmr spectroscopy |
JP2003096098A (ja) * | 2001-09-25 | 2003-04-03 | Nippon Sanso Corp | 安定同位体標識抗体及びその製造方法ならびにこれを用いた検出方法 |
US20030077572A1 (en) * | 2001-10-11 | 2003-04-24 | Abramson Fred P. | Quantitative differential display using isotope ratio monitoring |
WO2003049604A2 (en) * | 2001-12-13 | 2003-06-19 | The Johns Hopkins University School Of Medicine | Method for mr/nmr imaging |
EP1457482B9 (de) * | 2001-12-19 | 2012-10-24 | Japan Science and Technology Agency | Mit stabilen Isotopen markierte Aminosäure, Verfahren zu deren Einschleusung in ein Zielprotein, Verfahren zur NMR-Strukturanalyse von Protein |
WO2003104430A2 (en) * | 2002-06-10 | 2003-12-18 | Prospect Pharma | Method for obtaining dynamic and structural data pertaining to proteins and protein/ligand complexes |
WO2003106656A2 (en) | 2002-06-17 | 2003-12-24 | Thrasos, Inc. | Single domain tdf-related compounds and analogs thereof |
US7473535B2 (en) * | 2002-08-20 | 2009-01-06 | The Institute For Systems Biology | Chemical reagents and methods for detection and quantification of proteins in complex mixtures |
US7553455B1 (en) * | 2003-04-02 | 2009-06-30 | Sandia Corporation | Micromanifold assembly |
US7672791B2 (en) * | 2003-06-13 | 2010-03-02 | International Business Machines Corporation | Method of performing three-dimensional molecular superposition and similarity searches in databases of flexible molecules |
AU2004256368B2 (en) | 2003-07-11 | 2010-08-05 | Tatiana A. Egorova-Zachernyuk | Compositions and methods for stable isotope labelling of biological compounds |
US20070275442A1 (en) * | 2003-10-07 | 2007-11-29 | Prospect Pharma | Side Chain Deuterated Amino Acids Methods Of Use |
JP5019280B2 (ja) * | 2003-10-31 | 2012-09-05 | 独立行政法人科学技術振興機構 | 安定同位体標識芳香族アミノ酸、その標的蛋白質への組み込み方法並びに蛋白質のnmr構造解析方法 |
WO2005073747A1 (ja) * | 2004-02-02 | 2005-08-11 | Mitsubishi Chemical Corporation | Nmrシグナル帰属方法 |
EP2319526A1 (de) | 2004-06-17 | 2011-05-11 | Thrasos Therapeutics, Inc. | TDF-ähnliche Verbindungen und deren Analogal |
US7372895B2 (en) * | 2004-12-08 | 2008-05-13 | Telefonaktiebolaget Lm Ericsson (Publ) | Method of and system for delay estimation with minimized finger allocation |
US7480356B2 (en) * | 2004-12-08 | 2009-01-20 | Telefonaktiebolaget L M Ericsson (Publ) | Method of and system for path selection in rich multipath conditions |
CA2611324C (en) | 2005-06-07 | 2017-02-14 | Ocean Nutrition Canada Limited | Eukaryotic microorganisms for producing lipids and antioxidants |
WO2007035872A2 (en) | 2005-09-20 | 2007-03-29 | Thrasos Therapeutics, Inc. | Tdf-related compounds and analogs thereof |
US20070196280A1 (en) * | 2006-02-21 | 2007-08-23 | The Government Of The U.S.A. As Represented By The Sec. Of The Dept. Of Health And Human Services | In vivo magnetic resonance spectroscopy of aspartate transaminase activity |
GB0604647D0 (en) * | 2006-03-08 | 2006-04-19 | Shchepinov Mikhail | Stabilized food supplements and their derivatives |
JP2007255910A (ja) * | 2006-03-20 | 2007-10-04 | Hokkaido Univ | Nmrシグナルの帰属方法 |
WO2008129358A2 (en) * | 2006-08-01 | 2008-10-30 | Ocean Nutrition Canada Ltd. | Oil producing microbes and methods of modification thereof |
DK2276774T3 (en) | 2008-03-14 | 2016-11-28 | Retrotope Inc | Drugs that modulate genome methylation. |
WO2009114809A1 (en) * | 2008-03-14 | 2009-09-17 | Retrotope, Inc. | Therapies for cancer using isotopically substituted lysine |
US10052299B2 (en) | 2009-10-30 | 2018-08-21 | Retrotope, Inc. | Alleviating oxidative stress disorders with PUFA derivatives |
CN101914030B (zh) * | 2010-07-23 | 2013-04-24 | 上海化工研究院 | 一种13c,15n双标记甘氨酸的合成方法 |
EP2701698B1 (de) | 2011-04-26 | 2020-12-23 | Retrotope, Inc. | Erkrankungen mit pufa-oxidierung |
DK2701697T3 (da) | 2011-04-26 | 2020-06-29 | Retrotope Inc | Oxidative nethindesygdomme |
KR102111997B1 (ko) | 2011-04-26 | 2020-05-18 | 레트로토프 인코포레이티드 | Pufa와 관련된 신경퇴행성 질환 및 근육 질환 |
AU2012249918B2 (en) | 2011-04-26 | 2017-06-15 | Biojiva Llc | Impaired energy processing disorders and mitochondrial deficiency |
AU2014229307B2 (en) | 2013-03-13 | 2017-08-31 | Dsm Nutritional Products Ag | Engineering microorganisms |
WO2017091279A1 (en) | 2015-11-23 | 2017-06-01 | Retrotope, Inc. | Site-specific isotopic labeling of 1, 4-diene systems |
FR3055337A1 (fr) * | 2016-08-24 | 2018-03-02 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | Methode de preparation de milieux de culture riches pour le marquage d'acides amines incorpores dans des proteines |
WO2021168311A1 (en) | 2020-02-21 | 2021-08-26 | Retrotope, Inc. | Processes for isotopic modification of polyunsaturated fatty acids and derivatives thereof |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0768446B2 (ja) * | 1988-11-09 | 1995-07-26 | 三菱化学株式会社 | 芳香族ポリカーボネート樹脂組成物を用いた医療用部品 |
JP2518917B2 (ja) | 1989-02-07 | 1996-07-31 | 日本電子株式会社 | 蛋白質の構造解析方法 |
WO1990015525A1 (en) | 1989-06-14 | 1990-12-27 | Martek Corporation | Media for cell growth and method for making them |
JP2517688B2 (ja) * | 1989-12-25 | 1996-07-24 | 日本電子株式会社 | 3次元核磁気共鳴スペクトルの対称化処理方法 |
JPH0446143A (ja) * | 1990-06-11 | 1992-02-17 | Hitachi Ltd | 同位体多重標識アミノ酸の合成法 |
US5393669A (en) * | 1993-02-05 | 1995-02-28 | Martek Biosciences Corp. | Compositions and methods for protein structural determinations |
US5698401A (en) * | 1995-11-14 | 1997-12-16 | Abbott Laboratories | Use of nuclear magnetic resonance to identify ligands to target biomolecules |
US5891643A (en) | 1995-11-14 | 1999-04-06 | Abbott Laboratories | Use of nuclear magnetic resonance to design ligands to target biomolecules |
US6043024A (en) | 1997-04-18 | 2000-03-28 | Abbott Laboratories | Use of one-dimensional nuclear magnetic resonance to identify ligands to target biomolecules |
US6111066A (en) | 1997-09-02 | 2000-08-29 | Martek Biosciences Corporation | Peptidic molecules which have been isotopically substituted with 13 C, 15 N and 2 H in the backbone but not in the sidechains |
-
1997
- 1997-09-02 US US08/921,554 patent/US6111066A/en not_active Expired - Lifetime
-
1998
- 1998-09-01 ZA ZA9807975A patent/ZA987975B/xx unknown
- 1998-09-02 JP JP2000508633A patent/JP2001514239A/ja active Pending
- 1998-09-02 AT AT98945835T patent/ATE420061T1/de not_active IP Right Cessation
- 1998-09-02 EP EP07120079A patent/EP1889823A1/de not_active Withdrawn
- 1998-09-02 CA CA002301046A patent/CA2301046C/en not_active Expired - Fee Related
- 1998-09-02 AU AU92988/98A patent/AU754232B2/en not_active Ceased
- 1998-09-02 TW TW087114552A patent/TW581772B/zh not_active IP Right Cessation
- 1998-09-02 US US09/486,837 patent/US6335196B1/en not_active Expired - Lifetime
- 1998-09-02 HU HU0003383A patent/HUP0003383A3/hu unknown
- 1998-09-02 WO PCT/US1998/018197 patent/WO1999011589A1/en active IP Right Grant
- 1998-09-02 EP EP98945835A patent/EP1009725B1/de not_active Expired - Lifetime
- 1998-09-02 DE DE69840448T patent/DE69840448D1/de not_active Expired - Lifetime
-
2000
- 2000-03-01 NO NO20001052A patent/NO20001052L/no not_active Application Discontinuation
- 2000-07-10 US US09/613,571 patent/US6340578B1/en not_active Expired - Lifetime
- 2000-07-10 US US09/613,570 patent/US6376253B1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
WO1999011589A1 (en) | 1999-03-11 |
JP2001514239A (ja) | 2001-09-11 |
DE69840448D1 (de) | 2009-02-26 |
AU9298898A (en) | 1999-03-22 |
HUP0003383A2 (hu) | 2001-02-28 |
US6340578B1 (en) | 2002-01-22 |
AU754232B2 (en) | 2002-11-07 |
HUP0003383A3 (en) | 2003-07-28 |
TW581772B (en) | 2004-04-01 |
US6376253B1 (en) | 2002-04-23 |
EP1009725B1 (de) | 2009-01-07 |
ZA987975B (en) | 2000-01-21 |
US6335196B1 (en) | 2002-01-01 |
EP1009725A1 (de) | 2000-06-21 |
NO20001052L (no) | 2000-04-28 |
NO20001052D0 (no) | 2000-03-01 |
CA2301046C (en) | 2009-03-10 |
EP1889823A1 (de) | 2008-02-20 |
CA2301046A1 (en) | 1999-03-11 |
US6111066A (en) | 2000-08-29 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |