ATE420061T1 - 13c,15n,2h markierte proteine zur nmr strukturbestimmung und deren herstellung - Google Patents

13c,15n,2h markierte proteine zur nmr strukturbestimmung und deren herstellung

Info

Publication number
ATE420061T1
ATE420061T1 AT98945835T AT98945835T ATE420061T1 AT E420061 T1 ATE420061 T1 AT E420061T1 AT 98945835 T AT98945835 T AT 98945835T AT 98945835 T AT98945835 T AT 98945835T AT E420061 T1 ATE420061 T1 AT E420061T1
Authority
AT
Austria
Prior art keywords
labeled proteins
production
methods
labeled
proteins
Prior art date
Application number
AT98945835T
Other languages
English (en)
Inventor
Frank Anderson
Jonathan Brown
Philip Coughlin
Steven Homans
Charles Weller
Carrie Zimmerman-Wilson
Eddie Oliver
Original Assignee
Martek Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Martek Biosciences Corp filed Critical Martek Biosciences Corp
Application granted granted Critical
Publication of ATE420061T1 publication Critical patent/ATE420061T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B59/00—Introduction of isotopes of elements into organic compounds ; Labelled organic compounds per se
    • C07B59/008—Peptides; Proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R33/00—Arrangements or instruments for measuring magnetic variables
    • G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
    • G01R33/44—Arrangements or instruments for measuring magnetic variables involving magnetic resonance using nuclear magnetic resonance [NMR]
    • G01R33/46—NMR spectroscopy
    • G01R33/465—NMR spectroscopy applied to biological material, e.g. in vitro testing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • High Energy & Nuclear Physics (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Reproductive Health (AREA)
  • Virology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT98945835T 1997-09-02 1998-09-02 13c,15n,2h markierte proteine zur nmr strukturbestimmung und deren herstellung ATE420061T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/921,554 US6111066A (en) 1997-09-02 1997-09-02 Peptidic molecules which have been isotopically substituted with 13 C, 15 N and 2 H in the backbone but not in the sidechains

Publications (1)

Publication Number Publication Date
ATE420061T1 true ATE420061T1 (de) 2009-01-15

Family

ID=25445613

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98945835T ATE420061T1 (de) 1997-09-02 1998-09-02 13c,15n,2h markierte proteine zur nmr strukturbestimmung und deren herstellung

Country Status (12)

Country Link
US (4) US6111066A (de)
EP (2) EP1889823A1 (de)
JP (1) JP2001514239A (de)
AT (1) ATE420061T1 (de)
AU (1) AU754232B2 (de)
CA (1) CA2301046C (de)
DE (1) DE69840448D1 (de)
HU (1) HUP0003383A3 (de)
NO (1) NO20001052L (de)
TW (1) TW581772B (de)
WO (1) WO1999011589A1 (de)
ZA (1) ZA987975B (de)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6111066A (en) 1997-09-02 2000-08-29 Martek Biosciences Corporation Peptidic molecules which have been isotopically substituted with 13 C, 15 N and 2 H in the backbone but not in the sidechains
US6882939B2 (en) * 2000-10-20 2005-04-19 Prospect Pharma Rapid determination of protein global folds
EP1328815A1 (de) * 2000-10-20 2003-07-23 Martek Biosciences Corporation Schnelle bestimmung der global folds von proteinen
AU2002332390A1 (en) * 2001-03-12 2003-03-03 Affinium Pharmaceuticals, Inc. Proteins, druggable regions of proteins and target analysis for chemistry of therapeutics
WO2003006488A1 (en) * 2001-07-13 2003-01-23 The Regents Of The University Of California In-cell nmr spectroscopy
JP2003096098A (ja) * 2001-09-25 2003-04-03 Nippon Sanso Corp 安定同位体標識抗体及びその製造方法ならびにこれを用いた検出方法
US20030077572A1 (en) * 2001-10-11 2003-04-24 Abramson Fred P. Quantitative differential display using isotope ratio monitoring
WO2003049604A2 (en) * 2001-12-13 2003-06-19 The Johns Hopkins University School Of Medicine Method for mr/nmr imaging
EP1457482B9 (de) * 2001-12-19 2012-10-24 Japan Science and Technology Agency Mit stabilen Isotopen markierte Aminosäure, Verfahren zu deren Einschleusung in ein Zielprotein, Verfahren zur NMR-Strukturanalyse von Protein
WO2003104430A2 (en) * 2002-06-10 2003-12-18 Prospect Pharma Method for obtaining dynamic and structural data pertaining to proteins and protein/ligand complexes
WO2003106656A2 (en) 2002-06-17 2003-12-24 Thrasos, Inc. Single domain tdf-related compounds and analogs thereof
US7473535B2 (en) * 2002-08-20 2009-01-06 The Institute For Systems Biology Chemical reagents and methods for detection and quantification of proteins in complex mixtures
US7553455B1 (en) * 2003-04-02 2009-06-30 Sandia Corporation Micromanifold assembly
US7672791B2 (en) * 2003-06-13 2010-03-02 International Business Machines Corporation Method of performing three-dimensional molecular superposition and similarity searches in databases of flexible molecules
AU2004256368B2 (en) 2003-07-11 2010-08-05 Tatiana A. Egorova-Zachernyuk Compositions and methods for stable isotope labelling of biological compounds
US20070275442A1 (en) * 2003-10-07 2007-11-29 Prospect Pharma Side Chain Deuterated Amino Acids Methods Of Use
JP5019280B2 (ja) * 2003-10-31 2012-09-05 独立行政法人科学技術振興機構 安定同位体標識芳香族アミノ酸、その標的蛋白質への組み込み方法並びに蛋白質のｎｍｒ構造解析方法
WO2005073747A1 (ja) * 2004-02-02 2005-08-11 Mitsubishi Chemical Corporation Ｎｍｒシグナル帰属方法
EP2319526A1 (de) 2004-06-17 2011-05-11 Thrasos Therapeutics, Inc. TDF-ähnliche Verbindungen und deren Analogal
US7372895B2 (en) * 2004-12-08 2008-05-13 Telefonaktiebolaget Lm Ericsson (Publ) Method of and system for delay estimation with minimized finger allocation
US7480356B2 (en) * 2004-12-08 2009-01-20 Telefonaktiebolaget L M Ericsson (Publ) Method of and system for path selection in rich multipath conditions
CA2611324C (en) 2005-06-07 2017-02-14 Ocean Nutrition Canada Limited Eukaryotic microorganisms for producing lipids and antioxidants
WO2007035872A2 (en) 2005-09-20 2007-03-29 Thrasos Therapeutics, Inc. Tdf-related compounds and analogs thereof
US20070196280A1 (en) * 2006-02-21 2007-08-23 The Government Of The U.S.A. As Represented By The Sec. Of The Dept. Of Health And Human Services In vivo magnetic resonance spectroscopy of aspartate transaminase activity
GB0604647D0 (en) * 2006-03-08 2006-04-19 Shchepinov Mikhail Stabilized food supplements and their derivatives
JP2007255910A (ja) * 2006-03-20 2007-10-04 Hokkaido Univ Ｎｍｒシグナルの帰属方法
WO2008129358A2 (en) * 2006-08-01 2008-10-30 Ocean Nutrition Canada Ltd. Oil producing microbes and methods of modification thereof
DK2276774T3 (en) 2008-03-14 2016-11-28 Retrotope Inc Drugs that modulate genome methylation.
WO2009114809A1 (en) * 2008-03-14 2009-09-17 Retrotope, Inc. Therapies for cancer using isotopically substituted lysine
US10052299B2 (en) 2009-10-30 2018-08-21 Retrotope, Inc. Alleviating oxidative stress disorders with PUFA derivatives
CN101914030B (zh) * 2010-07-23 2013-04-24 上海化工研究院 一种13c，15n双标记甘氨酸的合成方法
EP2701698B1 (de) 2011-04-26 2020-12-23 Retrotope, Inc. Erkrankungen mit pufa-oxidierung
DK2701697T3 (da) 2011-04-26 2020-06-29 Retrotope Inc Oxidative nethindesygdomme
KR102111997B1 (ko) 2011-04-26 2020-05-18 레트로토프 인코포레이티드 Ｐｕｆａ와 관련된 신경퇴행성 질환 및 근육 질환
AU2012249918B2 (en) 2011-04-26 2017-06-15 Biojiva Llc Impaired energy processing disorders and mitochondrial deficiency
AU2014229307B2 (en) 2013-03-13 2017-08-31 Dsm Nutritional Products Ag Engineering microorganisms
WO2017091279A1 (en) 2015-11-23 2017-06-01 Retrotope, Inc. Site-specific isotopic labeling of 1, 4-diene systems
FR3055337A1 (fr) * 2016-08-24 2018-03-02 Commissariat A L'energie Atomique Et Aux Energies Alternatives Methode de preparation de milieux de culture riches pour le marquage d'acides amines incorpores dans des proteines
WO2021168311A1 (en) 2020-02-21 2021-08-26 Retrotope, Inc. Processes for isotopic modification of polyunsaturated fatty acids and derivatives thereof

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0768446B2 (ja) * 1988-11-09 1995-07-26 三菱化学株式会社 芳香族ポリカーボネート樹脂組成物を用いた医療用部品
JP2518917B2 (ja) 1989-02-07 1996-07-31 日本電子株式会社 蛋白質の構造解析方法
WO1990015525A1 (en) 1989-06-14 1990-12-27 Martek Corporation Media for cell growth and method for making them
JP2517688B2 (ja) * 1989-12-25 1996-07-24 日本電子株式会社 ３次元核磁気共鳴スペクトルの対称化処理方法
JPH0446143A (ja) * 1990-06-11 1992-02-17 Hitachi Ltd 同位体多重標識アミノ酸の合成法
US5393669A (en) * 1993-02-05 1995-02-28 Martek Biosciences Corp. Compositions and methods for protein structural determinations
US5698401A (en) * 1995-11-14 1997-12-16 Abbott Laboratories Use of nuclear magnetic resonance to identify ligands to target biomolecules
US5891643A (en) 1995-11-14 1999-04-06 Abbott Laboratories Use of nuclear magnetic resonance to design ligands to target biomolecules
US6043024A (en) 1997-04-18 2000-03-28 Abbott Laboratories Use of one-dimensional nuclear magnetic resonance to identify ligands to target biomolecules
US6111066A (en) 1997-09-02 2000-08-29 Martek Biosciences Corporation Peptidic molecules which have been isotopically substituted with 13 C, 15 N and 2 H in the backbone but not in the sidechains

Also Published As

Publication number Publication date
WO1999011589A1 (en) 1999-03-11
JP2001514239A (ja) 2001-09-11
DE69840448D1 (de) 2009-02-26
AU9298898A (en) 1999-03-22
HUP0003383A2 (hu) 2001-02-28
US6340578B1 (en) 2002-01-22
AU754232B2 (en) 2002-11-07
HUP0003383A3 (en) 2003-07-28
TW581772B (en) 2004-04-01
US6376253B1 (en) 2002-04-23
EP1009725B1 (de) 2009-01-07
ZA987975B (en) 2000-01-21
US6335196B1 (en) 2002-01-01
EP1009725A1 (de) 2000-06-21
NO20001052L (no) 2000-04-28
NO20001052D0 (no) 2000-03-01
CA2301046C (en) 2009-03-10
EP1889823A1 (de) 2008-02-20
CA2301046A1 (en) 1999-03-11
US6111066A (en) 2000-08-29

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