ATE400647T1 - C-terminale modifikation von polypeptiden - Google Patents

C-terminale modifikation von polypeptiden

Info

Publication number
ATE400647T1
ATE400647T1 AT05774367T AT05774367T ATE400647T1 AT E400647 T1 ATE400647 T1 AT E400647T1 AT 05774367 T AT05774367 T AT 05774367T AT 05774367 T AT05774367 T AT 05774367T AT E400647 T1 ATE400647 T1 AT E400647T1
Authority
AT
Austria
Prior art keywords
polypeptides
trypsin
xaa2
terminal modification
amino acid
Prior art date
Application number
AT05774367T
Other languages
English (en)
Inventor
Eva Hoess
Frank Bordusa
Rupert Herrmann
Hans-Dieter Jakubke
Wilhelm Tischer
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE400647T1 publication Critical patent/ATE400647T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AT05774367T 2004-08-13 2005-08-12 C-terminale modifikation von polypeptiden ATE400647T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP04019237 2004-08-13

Publications (1)

Publication Number Publication Date
ATE400647T1 true ATE400647T1 (de) 2008-07-15

Family

ID=34926160

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05774367T ATE400647T1 (de) 2004-08-13 2005-08-12 C-terminale modifikation von polypeptiden

Country Status (10)

Country Link
US (2) US20080064079A1 (de)
EP (1) EP1778839B1 (de)
JP (1) JP4538501B2 (de)
CN (1) CN101010425B (de)
AT (1) ATE400647T1 (de)
CA (1) CA2569707C (de)
DE (1) DE602005008071D1 (de)
ES (1) ES2309785T3 (de)
HK (1) HK1110888A1 (de)
WO (1) WO2006015879A1 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102005046113A1 (de) * 2005-09-27 2007-03-29 Sanofi-Aventis Deutschland Gmbh Verfahren zur Amidierung von Polypetiden mit C-terminalen basischen Aminosäuren unter Verwendung spezifischer Endoproteasen
DE102006031962A1 (de) * 2006-07-11 2008-01-17 Sanofi-Aventis Deutschland Gmbh Amidiertes Insulin Glargin
DE102006031955A1 (de) 2006-07-11 2008-01-17 Sanofi-Aventis Deutschland Gmbh Verfahren zur Herstellung von Insulinanaloga mit dibasischem B-Kettenende
PT3228320T (pt) 2008-10-17 2020-03-26 Sanofi Aventis Deutschland Combinação de uma insulina e de um agonista do glp-1
JP5770161B2 (ja) 2009-04-06 2015-08-26 ノヴォ ノルディスク アー／エス 血小板への第ｖｉｉｉ因子タンパク質の標的送達
AU2010317994B2 (en) 2009-11-13 2014-03-06 Sanofi-Aventis Deutschland Gmbh Pharmaceutical composition comprising a GLP-1 agonist and methionine
MY180661A (en) 2009-11-13 2020-12-04 Sanofi Aventis Deutschland Pharmaceutical composition comprising a glp-1 agonist, an insulin and methionine
EP2533813B1 (de) 2010-02-11 2016-06-01 F.Hoffmann-La Roche Ag Proteinkonjugate für trypsinvermittelte Pegylierung durch Transamidierung und Methoden
US20140148384A1 (en) 2010-08-30 2014-05-29 Sanofi-Aventis Deutschland Gmbh Use of ave0010 for the manufacture of a medicament for the treatment of diabetes mellitus type 2
US9821032B2 (en) 2011-05-13 2017-11-21 Sanofi-Aventis Deutschland Gmbh Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
BR112014004726A2 (pt) 2011-08-29 2017-04-04 Sanofi Aventis Deutschland combinação farmacêutica para uso no controle glicêmico em pacientes de diabetes tipo 2
AR087744A1 (es) 2011-09-01 2014-04-16 Sanofi Aventis Deutschland Composicion farmaceutica para uso en el tratamiento de una enfermedad neurodegenerativa
EP3229828B1 (de) 2014-12-12 2023-04-05 Sanofi-Aventis Deutschland GmbH Insulin-glargin/lixisenatid-fixierte verhältnisformulierung
TWI748945B (zh) 2015-03-13 2021-12-11 德商賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患治療
TW201705975A (zh) 2015-03-18 2017-02-16 賽諾菲阿凡提斯德意志有限公司 第２型糖尿病病患之治療
US10738338B2 (en) 2016-10-18 2020-08-11 The Research Foundation for the State University Method and composition for biocatalytic protein-oligonucleotide conjugation and protein-oligonucleotide conjugate
TW202015723A (zh) 2018-05-18 2020-05-01 美商百歐維拉提夫治療公司 治療ａ型血友病的方法
EP3650541A1 (de) 2018-11-08 2020-05-13 BioPharma Translationsinstitut Dessau Forschungs GmbH Trypsin-mutante zur c- und n-terminalen modifizierung von polypeptiden
US20220235344A1 (en) * 2018-12-19 2022-07-28 Biopharma Translationsinstitut Dessau Forschungs Gmbh Trypsin variants with improved enzymatic properties
AU2020399194A1 (en) 2019-12-10 2022-07-28 Sanofi A method of forming a conjugate of a sulfonamide and a polypeptide

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9001705D0 (sv) * 1990-05-11 1990-05-11 Medscand Ab Saett foer diagnostik av virusbaerande tumoerer genom immunoassay
US5580751A (en) * 1990-09-14 1996-12-03 Carlsberg A/S Process for the preparation of C-terminally amidated peptides
AU6171594A (en) 1993-02-04 1994-08-29 Genentech Inc. Serine protease variants having peptide ligase activity
US6187579B1 (en) * 1993-10-28 2001-02-13 Carlsberg A/S Customized proteases
US5958783A (en) 1994-07-25 1999-09-28 Roche Diagnostics Gmbh Metal complexes with a charged linker
US5985627A (en) 1997-02-28 1999-11-16 Carlsberg Laboratory Modified carboxypeptidase
DE10049673A1 (de) * 2000-10-07 2002-04-25 Univ Leipzig Verfahren zur Herstellung von Peptiden, Peptidmimetika und Proteinen
CN1354253A (zh) * 2001-12-24 2002-06-19 南京医科大学 淡色库蚊胰蛋白酶基因

Also Published As

Publication number Publication date
CN101010425A (zh) 2007-08-01
WO2006015879A1 (en) 2006-02-16
US8927230B2 (en) 2015-01-06
HK1110888A1 (en) 2008-07-25
JP2008508871A (ja) 2008-03-27
CA2569707C (en) 2013-09-24
EP1778839B1 (de) 2008-07-09
US20080064079A1 (en) 2008-03-13
JP4538501B2 (ja) 2010-09-08
US20130177940A1 (en) 2013-07-11
ES2309785T3 (es) 2008-12-16
CA2569707A1 (en) 2006-02-16
EP1778839A1 (de) 2007-05-02
DE602005008071D1 (de) 2008-08-21
CN101010425B (zh) 2010-05-26

Similar Documents

Publication Publication Date Title
ATE400647T1 (de) C-terminale modifikation von polypeptiden
FR2831170B1 (fr) Proteines c modifiees activables directement par la thrombine
MY148447A (en) Method for amidating polypeptides with basic aminoacid d-terminals by means of specific endoproteases
NZ597685A (en) Proteins and nucleic acids from meningitis/sepsis-associated escherichia coli
IN2014CN02050A (de)
BRPI0606612A2 (pt) expressão recombinante de proteìnas em uma forma de duas cadeias ligadas por dissulfeto
DK1144607T5 (da) Fremgangsmåde til præsentation af (poly)peptider/proteiner på bakteriofage partikler via disulfidbindinger
NO20074374L (no) Fremgangsmate for fremstilling av polypeptidblandinger ved anvendelse av hydrogenolyse
NZ593837A (en) Recombinantly modified plasmin
CA2443365A1 (en) Methods for the recombinant production of antifusogenic peptides
EP2348053A3 (de) Oligopeptid-Liganden
MXPA03000786A (es) Peptidos de promiostatina y metodos de usar los mismos.
ATE398179T1 (de) Von rna polymerasen abstammende polypeptide und ihre verwendungen
WO2002038742A3 (en) Dipeptidylpeptidases and methods of use
EP2014676A4 (de) Mutante des hgf-precursor-proteins und aktivierte form davon
MX2018009216A (es) Metodo para producir un hidrolizado de proteina empleando una tripeptidil peptidasa de aspergillus fumigatus.
DE60134230D1 (de) Substraten und nachweise zur wirksamkeit von beta-secretase
NZ601647A (en) Variant hhip1 protein and methods and uses thereof
BRPI0715331B8 (pt) polipeptídeo que compreende uma porção n-terminal e uma porção c-terminal, e uso do mesmo
ATE485831T1 (de) Behandlung von neurodegeneration
EP1915617A4 (de) Verfahren und zusammensetzungen zur identifizierung eines peptids mit intermolekularer wechselwirkung mit einem interessierenden ziel
SG170757A1 (en) Polypeptide having esterase activity and recombinant esterase and use thereof
WO2009118710A3 (en) Cd4-related polypeptides and methods of use
WO2021188869A3 (en) Determination of free n-terminus of pegfilgrastim using an acid protease

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1778839

Country of ref document: EP