ATE355522T1 - METHOD FOR MEASURING THE ACTIVITY OF G(ALPHA)I- OR G(ALPHA)O-COUPLED RECEPTORS USING CA2+ INFLUX IN CELLS - Google Patents
METHOD FOR MEASURING THE ACTIVITY OF G(ALPHA)I- OR G(ALPHA)O-COUPLED RECEPTORS USING CA2+ INFLUX IN CELLSInfo
- Publication number
- ATE355522T1 ATE355522T1 AT03789105T AT03789105T ATE355522T1 AT E355522 T1 ATE355522 T1 AT E355522T1 AT 03789105 T AT03789105 T AT 03789105T AT 03789105 T AT03789105 T AT 03789105T AT E355522 T1 ATE355522 T1 AT E355522T1
- Authority
- AT
- Austria
- Prior art keywords
- alpha
- measuring
- influx
- cells
- activity
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/88—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving prostaglandins or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
- G01N33/9406—Neurotransmitters
- G01N33/9413—Dopamine
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
- G01N33/9406—Neurotransmitters
- G01N33/942—Serotonin, i.e. 5-hydroxy-tryptamine
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
- G01N33/9406—Neurotransmitters
- G01N33/9426—GABA, i.e. gamma-amino-butyrate
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
- G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pharmacology & Pharmacy (AREA)
- Toxicology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A method of measuring the activation or deactivation of G(alpha)i- or G(alpha)o-coupled receptors, and methods of identifying agonists or antagonists of such receptors.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10256947A DE10256947A1 (en) | 2002-12-05 | 2002-12-05 | Method for measuring the activity of G (alpha) i- or G (alpha) o-coupled receptors using Ca2 + influx in cells |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE355522T1 true ATE355522T1 (en) | 2006-03-15 |
Family
ID=32336022
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT03789105T ATE355522T1 (en) | 2002-12-05 | 2003-12-04 | METHOD FOR MEASURING THE ACTIVITY OF G(ALPHA)I- OR G(ALPHA)O-COUPLED RECEPTORS USING CA2+ INFLUX IN CELLS |
Country Status (7)
Country | Link |
---|---|
US (2) | US20050277110A1 (en) |
EP (1) | EP1570266B1 (en) |
AT (1) | ATE355522T1 (en) |
AU (1) | AU2003293742A1 (en) |
DE (2) | DE10256947A1 (en) |
ES (1) | ES2282718T3 (en) |
WO (1) | WO2004051264A1 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2034977B1 (en) | 2006-04-21 | 2011-07-13 | DSM IP Assets B.V. | Use of opioid receptor antagonists |
CN109946451B (en) * | 2019-04-16 | 2022-05-20 | 浙江诺迦生物科技有限公司 | Detection method and detection kit for cannabinoids active substances based on concentration change effect of intracellular free calcium ions |
CN109946450B (en) * | 2019-04-16 | 2022-04-19 | 浙江诺迦生物科技有限公司 | Detection method and detection kit for opioid active substances based on concentration change effect of intracellular free calcium ions |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6004808A (en) * | 1996-06-21 | 1999-12-21 | Aurora Biosciences Corporation | Promiscuous G-protein compositions and their use |
US6383761B2 (en) * | 1997-07-28 | 2002-05-07 | The Regents Of The University Of California | Methods and compositions for identifying modulators of G-protein-coupled receptors |
US6709830B2 (en) * | 1997-11-13 | 2004-03-23 | The Regents Of The University Of California | Methods for modulating the activation of a lymphocyte expressed G protein coupled receptor involved in cell proliferation, autoimmunity and inflammation |
GB9903767D0 (en) * | 1999-02-18 | 1999-04-14 | Univ Glasgow | Receptor assay |
AU2000273414A1 (en) * | 2000-02-07 | 2001-08-20 | Tropix, Inc. | Enzyme-based gprotein-coupled receptor assay |
ATE330027T1 (en) * | 2000-03-13 | 2006-07-15 | Merck & Co Inc | HIGH THROUGHPUT SCREENING BY MEASURING INTRACELLULAR CALCIUM LEVELS |
US6448377B1 (en) * | 2000-09-27 | 2002-09-10 | The Board Of Trustees Of The Leland Stanford Junior University | Modified G protein sunbunits |
DE10123958A1 (en) * | 2001-05-17 | 2002-11-28 | Aventis Pharma Gmbh | Identifying ligands for G protein-coupled receptors, useful for treating cardiovascular disease, by using calcium-sensitive fluorophores in cells expressing G alpha proteins |
-
2002
- 2002-12-05 DE DE10256947A patent/DE10256947A1/en not_active Ceased
-
2003
- 2003-12-04 DE DE50306697T patent/DE50306697D1/en not_active Expired - Lifetime
- 2003-12-04 EP EP03789105A patent/EP1570266B1/en not_active Expired - Lifetime
- 2003-12-04 ES ES03789105T patent/ES2282718T3/en not_active Expired - Lifetime
- 2003-12-04 WO PCT/EP2003/013510 patent/WO2004051264A1/en active IP Right Grant
- 2003-12-04 AU AU2003293742A patent/AU2003293742A1/en not_active Abandoned
- 2003-12-04 AT AT03789105T patent/ATE355522T1/en active
-
2005
- 2005-06-03 US US11/143,456 patent/US20050277110A1/en not_active Abandoned
-
2008
- 2008-12-16 US US12/336,025 patent/US7829300B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
AU2003293742A1 (en) | 2004-06-23 |
ES2282718T3 (en) | 2007-10-16 |
EP1570266A1 (en) | 2005-09-07 |
US20050277110A1 (en) | 2005-12-15 |
EP1570266B1 (en) | 2007-02-28 |
DE50306697D1 (en) | 2007-04-12 |
US7829300B2 (en) | 2010-11-09 |
US20090136989A1 (en) | 2009-05-28 |
WO2004051264A1 (en) | 2004-06-17 |
DE10256947A1 (en) | 2004-06-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE278803T1 (en) | METHOD FOR MONITORING PROTEASOME INHIBITOR DRUG EFFECTS | |
DE60334599D1 (en) | Method and device for dialog management between user and agent | |
DE60329377D1 (en) | METHOD OF FORECASTING SEPSIS IN A PATIENT WITH SYSTEMIC INFLAMMATORY RESPONSE SYNDROME (SIRS) | |
ATE480674T1 (en) | METHOD AND SYSTEM FOR PERFORMING DETERMINATION OF THE POSITION OF A DEVICE | |
ATE385562T1 (en) | MONITORING SYSTEM AND METHOD OF USE THEREOF | |
DE60324539D1 (en) | DEVICE FOR SUPPORTING THE MEMORY AREA NETWORK SYSTEM CONSTRUCTION, METHOD FOR SUPPORTING THE SYSTEM CONSTRUCTION, AND PROGRAM FOR SUPPORTING THE SYSTEM CONSTRUCTION | |
DE50308558D1 (en) | DEVICE FOR MEASURING BENTIFICITY OF FLAT SUBMISSIONS | |
DE60309453D1 (en) | DEVICE FOR MANAGING A PATIENT TREATMENT PROGRAM | |
ATE464056T1 (en) | METHOD FOR ACTIVATION OF NKT CELLS | |
ATE456666T1 (en) | METHOD FOR EVALUATION OF RIBONUCLEOTIDE SEQUENCES | |
AR029838A1 (en) | SUBSTITUTED BENZONITROGEN HETEROCICLES | |
ATE403151T1 (en) | SUPPORT PLATE FOR PERFORMING FUNCTIONAL TESTS ON BIOLOGICAL CELLS AND METHOD FOR COATING THE SUPPORT PLATE | |
DE60326973D1 (en) | BIOACTIVE COATING FOR MEDICAL DEVICE WITH KERATIN | |
DE602007010246D1 (en) | PROCESS FOR BLOOD DETERMINATION | |
DE602006013604D1 (en) | METHOD FOR DETERMINING RESPONSIVENESS AGAINST CHK1 INHIBITORS | |
ATE355522T1 (en) | METHOD FOR MEASURING THE ACTIVITY OF G(ALPHA)I- OR G(ALPHA)O-COUPLED RECEPTORS USING CA2+ INFLUX IN CELLS | |
NZ594843A (en) | Method or system using biomarkers for the monitoring of a treatment | |
DE60315575D1 (en) | Method and device for the examination of surfaces | |
DE60303757D1 (en) | Method and system for measuring low impedances | |
ATE408211T1 (en) | METHOD FOR FORECASTING A TRAFFIC CONDITION IN A ROAD NETWORK AND TRAFFIC MANAGEMENT CENTRE | |
DE60318900D1 (en) | NUCLEIC ACIDS, POLYPEPTIDES AND METHOD FOR MODULATING APOPTOSIS | |
ATA11452002A (en) | METHOD AND DEVICE FOR MEASURING THE MACROMOLECULAR PROTON DENSITY | |
ATE392619T1 (en) | METHOD FOR DIAGNOSING SYNOVIAL OR OSTEOARTICULAR DISEASES | |
DE60323319D1 (en) | Apparatus and method for voice activity detection | |
DE50303478D1 (en) | TEST SYSTEM FOR EVALUATING THE COMPATIBILITY OF BIOLOGICALLY ACTIVE SUBSTANCES WITH COPOLYMERS |