ATE324439T1 - Zelllinien zur heterologen expression von genprodukten mit humanem glykosilierungsmuster - Google Patents

Zelllinien zur heterologen expression von genprodukten mit humanem glykosilierungsmuster

Info

Publication number
ATE324439T1
ATE324439T1 AT03776861T AT03776861T ATE324439T1 AT E324439 T1 ATE324439 T1 AT E324439T1 AT 03776861 T AT03776861 T AT 03776861T AT 03776861 T AT03776861 T AT 03776861T AT E324439 T1 ATE324439 T1 AT E324439T1
Authority
AT
Austria
Prior art keywords
cell
gene product
starting
dna sequence
high yield
Prior art date
Application number
AT03776861T
Other languages
English (en)
Inventor
Volker Sandig
Karsten Winkler
Uwe Marx
Tobias Wermelinger
Original Assignee
Probiogen Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Probiogen Ag filed Critical Probiogen Ag
Application granted granted Critical
Publication of ATE324439T1 publication Critical patent/ATE324439T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT03776861T 2002-10-04 2003-10-06 Zelllinien zur heterologen expression von genprodukten mit humanem glykosilierungsmuster ATE324439T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP02022194A EP1405908A1 (de) 2002-10-04 2002-10-04 Herstellung von Zelllinien zur heterologen Expression

Publications (1)

Publication Number Publication Date
ATE324439T1 true ATE324439T1 (de) 2006-05-15

Family

ID=31985049

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03776861T ATE324439T1 (de) 2002-10-04 2003-10-06 Zelllinien zur heterologen expression von genprodukten mit humanem glykosilierungsmuster

Country Status (9)

Country Link
US (1) US20060148085A1 (de)
EP (2) EP1405908A1 (de)
JP (1) JP2006500937A (de)
AT (1) ATE324439T1 (de)
AU (1) AU2003286135B2 (de)
CA (1) CA2501218A1 (de)
DE (1) DE60304881T2 (de)
DK (1) DK1546328T3 (de)
WO (1) WO2004031383A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ549622A (en) 2004-02-18 2010-09-30 Chromocell Corp Methods and materials using signaling probes
DK1805296T3 (da) * 2004-10-22 2010-01-18 Novozymes As Stabil genomisk integrering af multipie polynukleotidkopier
CA2716801A1 (en) * 2008-03-12 2009-09-17 Wyeth Llc Methods for identifying cells suitable for large-scale production of recombinant proteins
EP2105505A1 (de) 2008-03-28 2009-09-30 Celonic AG Verfahren und Materialien zur wiederholbaren Erzeugung hochproduktiver Zelllinien für rekombinante Proteine
EP2281048A4 (de) * 2008-04-21 2012-04-18 Unitargeting Res As Gentechnisch modifizierte eukaryontische zellen
EP2527448A1 (de) 2011-05-23 2012-11-28 Novozymes A/S Gleichzeitige standortspezifische Integrationen von mehreren Genkopien in faserige Pilze
FR2981946B1 (fr) * 2011-10-28 2015-02-20 Lfb Biotechnologies Unites de transcription et leur utilisation dans des vecteurs d'expression (yb2/0)
CN109804072A (zh) 2016-10-25 2019-05-24 诺维信公司 地衣芽孢杆菌中flp介导的基因组整合
GB201703417D0 (en) 2017-03-03 2017-04-19 Ge Healthcare Bio Sciences Ab Method for cell line development

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6130364A (en) * 1995-03-29 2000-10-10 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
US6086913A (en) * 1995-11-01 2000-07-11 University Of British Columbia Liposomal delivery of AAV vectors
US6534314B1 (en) * 1996-06-14 2003-03-18 Massachusetts Institute Of Technology Methods and compositions for transforming cells
AU745960C (en) * 1997-11-18 2003-09-18 Pioneer Hi-Bred International, Inc. A novel method for the integration of foreign DNA into eukaryoticgenomes
MXPA03000634A (es) * 2000-07-21 2004-12-03 Us Agriculture Metodos para el reemplazo, el desplazamiento y acumulacion de adn en genomas eucarioticos.

Also Published As

Publication number Publication date
US20060148085A1 (en) 2006-07-06
WO2004031383A1 (en) 2004-04-15
EP1546328A1 (de) 2005-06-29
EP1405908A1 (de) 2004-04-07
DE60304881T2 (de) 2007-04-19
DK1546328T3 (da) 2006-08-07
CA2501218A1 (en) 2004-04-15
AU2003286135B2 (en) 2009-01-15
EP1546328B1 (de) 2006-04-26
DE60304881D1 (de) 2006-06-01
JP2006500937A (ja) 2006-01-12
AU2003286135A1 (en) 2004-04-23

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