ATE306328T1 - APPARATUS AND METHOD FOR MIXING AND SEPARATING USING MAGNETIC PARTICLES - Google Patents
APPARATUS AND METHOD FOR MIXING AND SEPARATING USING MAGNETIC PARTICLESInfo
- Publication number
- ATE306328T1 ATE306328T1 AT00905525T AT00905525T ATE306328T1 AT E306328 T1 ATE306328 T1 AT E306328T1 AT 00905525 T AT00905525 T AT 00905525T AT 00905525 T AT00905525 T AT 00905525T AT E306328 T1 ATE306328 T1 AT E306328T1
- Authority
- AT
- Austria
- Prior art keywords
- container
- magnetic particles
- magnetic
- test medium
- magnet
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/28—Magnetic plugs and dipsticks
- B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/005—Pretreatment specially adapted for magnetic separation
- B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
- Sampling And Sample Adjustment (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
An apparatus and method for carrying out the affinity separation of a target substance from a liquid test medium by mixing magnetic particles having surface immobilized ligand or receptor within the test medium to promote an affinity binding reaction between the ligand and the target substance. The test medium with the magnetic particles in a suitable container is removably mounted in an apparatus that creates a magnetic field gradient in the test medium. This magnetic gradient is used to induce the magnetic particles to move, thereby effecting mixing. The mixing is achieved either by movement of a magnet relative to a stationary container or movement of the container relative to a stationary magnet. In either case, the magnetic particles experience a continuous angular position change with the magnet. Concurrently with the relative angular movement between the magnet and the magnetic particles, the magnet is also moved along the length of the container causing the magnetic field gradient to sweep the entire length of the container. After the desired time, sufficient for the affinity reaction to occur, movement of the magnetic gradient is ended, whereby the magnetic particles are immobilized on the inside wall of the container nearest to the magnetic source. The remaining test medium is removed while the magnetic particles are retained on the wall of the container. The test medium or the particles may then be subjected to further processing.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/US2000/000071 WO2001049419A1 (en) | 2000-01-04 | 2000-01-04 | Apparatus and method for mixing and separation employing magnetic particles |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE306328T1 true ATE306328T1 (en) | 2005-10-15 |
Family
ID=21740942
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT00905525T ATE306328T1 (en) | 2000-01-04 | 2000-01-04 | APPARATUS AND METHOD FOR MIXING AND SEPARATING USING MAGNETIC PARTICLES |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1248680B1 (en) |
JP (1) | JP4651894B2 (en) |
AT (1) | ATE306328T1 (en) |
DE (1) | DE60023185T2 (en) |
WO (1) | WO2001049419A1 (en) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2483445C (en) | 2002-04-26 | 2011-04-19 | Abbott Laboratories | Structure and method for handling magnetic particles in biological assays |
CN1280428C (en) * | 2003-05-19 | 2006-10-18 | 清华大学 | Biochip system based on minute particle and its application |
CN102764607B (en) * | 2006-06-21 | 2015-01-07 | 斯彼诺米克斯公司 | A device and method for processing and mixing magnetic particles in a liquid medium |
JP4586054B2 (en) * | 2007-08-31 | 2010-11-24 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
GB0717452D0 (en) * | 2007-09-07 | 2007-10-17 | Mole Genetics As | Separation apparatus |
JP2009066476A (en) * | 2007-09-11 | 2009-04-02 | Tamagawa Seiki Co Ltd | Method and device for collecting/dispersing magnetic particle in liquid |
US7852470B2 (en) | 2007-12-28 | 2010-12-14 | Morpho Detection, Inc. | System and method for improved biodetection |
US7847932B2 (en) | 2007-12-28 | 2010-12-07 | Morpho Detection, Inc. | System and method for improved biodetection |
US8053250B2 (en) * | 2008-06-27 | 2011-11-08 | Rex Chin-Yih Hong | Method and system for suppressing bindings on magnetic particles |
WO2010117458A1 (en) | 2009-04-10 | 2010-10-14 | President And Fellows Of Harvard College | Manipulation of particles in channels |
KR20140006024A (en) * | 2011-03-29 | 2014-01-15 | 용신 장 | Multifunctional bioreactor system and methods for cell sorting and culturing |
JP2012152213A (en) * | 2012-02-14 | 2012-08-16 | Tamagawa Seiki Co Ltd | Device for aggregating/dispersing magnetic particle in liquid |
JP2012152734A (en) * | 2012-02-14 | 2012-08-16 | Tamagawa Seiki Co Ltd | Assembly/dispersion apparatus of magnetic particle in liquid |
JP5347121B2 (en) * | 2012-02-14 | 2013-11-20 | 多摩川精機株式会社 | Device for collecting / dispersing magnetic particles in liquid |
WO2015177933A1 (en) * | 2014-05-23 | 2015-11-26 | 株式会社島津製作所 | Method for operating magnetic body particles and device for operating magnetic body particles |
JP6323550B2 (en) * | 2014-05-23 | 2018-05-16 | 株式会社島津製作所 | Operation method of magnetic particles |
JP2018161649A (en) * | 2018-04-10 | 2018-10-18 | 株式会社島津製作所 | Method and device for operating magnetic material particle |
US11406989B2 (en) * | 2018-04-25 | 2022-08-09 | Zymo Research Corporation | Apparatus and methods centrifugal and magnetic sample isolation |
EP3823761A4 (en) | 2018-07-20 | 2022-04-20 | Cornell University | Magnetic separation of biological entities from fluid sample |
CN110346556B (en) * | 2019-07-15 | 2023-02-17 | 深圳海思安生物技术有限公司 | Liquid detection reagent and using method thereof |
CN110423676B (en) * | 2019-08-13 | 2023-04-14 | 华东理工大学 | Magnetically controlled bioreactor system for in vitro cell culture |
CN115052676A (en) * | 2019-11-27 | 2022-09-13 | Jbs科学公司 | Method and device for mixing magnetic particles in a liquid medium |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3985649A (en) * | 1974-11-25 | 1976-10-12 | Eddelman Roy T | Ferromagnetic separation process and material |
JPS5753257A (en) * | 1980-09-16 | 1982-03-30 | Tohoku Metal Ind Ltd | Apparatus for separating magnetic particulate body |
JPS58193687A (en) * | 1982-05-10 | 1983-11-11 | Res Dev Corp Of Japan | Agitation and separation of microscopic material |
AU4927496A (en) * | 1995-02-21 | 1996-09-11 | Iqbal W. Siddiqi | Apparatus and method for mixing and separation employing magnetic particles |
-
2000
- 2000-01-04 JP JP2001549777A patent/JP4651894B2/en not_active Expired - Fee Related
- 2000-01-04 AT AT00905525T patent/ATE306328T1/en not_active IP Right Cessation
- 2000-01-04 DE DE60023185T patent/DE60023185T2/en not_active Expired - Lifetime
- 2000-01-04 EP EP00905525A patent/EP1248680B1/en not_active Expired - Lifetime
- 2000-01-04 WO PCT/US2000/000071 patent/WO2001049419A1/en active IP Right Grant
Also Published As
Publication number | Publication date |
---|---|
DE60023185D1 (en) | 2006-02-23 |
DE60023185T2 (en) | 2006-05-11 |
WO2001049419A1 (en) | 2001-07-12 |
JP4651894B2 (en) | 2011-03-16 |
EP1248680A1 (en) | 2002-10-16 |
JP2003519008A (en) | 2003-06-17 |
EP1248680B1 (en) | 2005-10-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |