ATE146223T1 - Stabile d-n-alpha-carbamylase mutanten - Google Patents
Stabile d-n-alpha-carbamylase mutantenInfo
- Publication number
- ATE146223T1 ATE146223T1 AT95103572T AT95103572T ATE146223T1 AT E146223 T1 ATE146223 T1 AT E146223T1 AT 95103572 T AT95103572 T AT 95103572T AT 95103572 T AT95103572 T AT 95103572T AT E146223 T1 ATE146223 T1 AT E146223T1
- Authority
- AT
- Austria
- Prior art keywords
- mutants
- alpha
- carbamylase
- stable
- preparation
- Prior art date
Links
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 150000001413 amino acids Chemical group 0.000 abstract 2
- 244000005700 microbiome Species 0.000 abstract 2
- 239000013612 plasmid Substances 0.000 abstract 2
- 150000007650 D alpha amino acids Chemical class 0.000 abstract 1
- 235000001014 amino acid Nutrition 0.000 abstract 1
- 235000018417 cysteine Nutrition 0.000 abstract 1
- 150000001945 cysteines Chemical group 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ITMI940725A IT1269321B (it) | 1994-04-15 | 1994-04-15 | Mutanti stabili della d-n- -carbamilasi |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE146223T1 true ATE146223T1 (de) | 1996-12-15 |
Family
ID=11368609
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT95103572T ATE146223T1 (de) | 1994-04-15 | 1995-03-13 | Stabile d-n-alpha-carbamylase mutanten |
Country Status (10)
Country | Link |
---|---|
US (2) | US5869298A (de) |
EP (1) | EP0677584B1 (de) |
JP (1) | JP3701341B2 (de) |
AT (1) | ATE146223T1 (de) |
DE (1) | DE69500108T2 (de) |
DK (1) | DK0677584T3 (de) |
ES (1) | ES2096494T3 (de) |
GR (1) | GR3022476T3 (de) |
IT (1) | IT1269321B (de) |
SI (1) | SI0677584T1 (de) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5824522A (en) * | 1990-12-07 | 1998-10-20 | Kanegafuchi Kagaku Kogyo Kabushiki Kaisha | Recombinant decarbamylases for producing D-α-amino acids |
IT1274168B (it) * | 1994-04-15 | 1997-07-15 | Eniricerche Spa | Vettore plasmidico e suo impiego per la produzione di proteine eterologhe |
US5962279A (en) * | 1994-06-24 | 1999-10-05 | Kanegafuchi Kagaku Kogyo Kabushiki Kaisha | Process for producing D-amino acids with composite immobilized enzyme preparation |
IT1277125B1 (it) * | 1995-12-21 | 1997-11-04 | Eniricerche Spa | Mutanti termostabili della d-n-alfa-carbamilasi |
US6524837B1 (en) | 1999-03-29 | 2003-02-25 | California Institute Of Technology | Hydantoinase variants with improved properties and their use for the production of amino acids |
EP1199355B1 (de) * | 1999-06-29 | 2007-03-14 | National Institute of Advanced Industrial Science and Technology | Schwefelfreie enzyme |
JP2001069981A (ja) * | 1999-08-31 | 2001-03-21 | Kanegafuchi Chem Ind Co Ltd | デカルバミラーゼの立体構造およびその利用法 |
WO2001079440A2 (en) * | 2000-04-13 | 2001-10-25 | Novozymes A/S | Extracellular expression of pectate lyase using bacillus or escherichia coli |
JP4561009B2 (ja) * | 2001-07-10 | 2010-10-13 | 味の素株式会社 | D−ヒダントインハイドロラーゼをコードするdna、n−カルバミル−d−アミノ酸ハイドロラーゼをコードするdna、該遺伝子を含む組み換えdna、該組み換えdnaにより形質転換された細胞、該形質転換細胞を用いるタンパク質の製造方法、および、d−アミノ酸の製造方法 |
JP4561010B2 (ja) * | 2001-07-10 | 2010-10-13 | 味の素株式会社 | D−ヒダントインハイドロラーゼをコードするdna、n−カルバミル−d−アミノ酸ハイドロラーゼをコードするdna、該遺伝子を含む組み換えdna、該組み換えdnaにより形質転換された細胞、該形質転換細胞を用いるタンパク質の製造方法、および、d−アミノ酸の製造方法 |
CN111454933B (zh) * | 2020-05-08 | 2022-08-16 | 江南大学 | D-氨甲酰水解酶突变体及其在d-芳香族氨基酸合成中的应用 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2526965B2 (ja) * | 1987-02-24 | 1996-08-21 | 武田薬品工業株式会社 | ムテイン,dnaおよびその用途 |
DE69129899T2 (de) * | 1990-12-07 | 1999-01-14 | Kanegafuchi Kagaku Kogyo K.K., Osaka | Verfahren zur Herstellung von D-Alpha-Aminosäuren |
EP1568778A1 (de) * | 1992-06-30 | 2005-08-31 | Smithkline Beecham Plc | Hydantoinase aus Agrobacterium |
EP0610517B1 (de) * | 1992-08-10 | 2004-10-20 | Kanegafuchi Kagaku Kogyo Kabushiki Kaisha | Für eine decarbamylase mit verbesserter thermostabilität kodierende dna und ihre verwendung |
IT1274167B (it) * | 1994-04-15 | 1997-07-15 | Eniricerche Spa | Procedimento per la produzione di d- -amminoacidi |
-
1994
- 1994-04-15 IT ITMI940725A patent/IT1269321B/it active IP Right Grant
-
1995
- 1995-03-13 DK DK95103572.4T patent/DK0677584T3/da active
- 1995-03-13 AT AT95103572T patent/ATE146223T1/de not_active IP Right Cessation
- 1995-03-13 DE DE69500108T patent/DE69500108T2/de not_active Expired - Fee Related
- 1995-03-13 EP EP95103572A patent/EP0677584B1/de not_active Expired - Lifetime
- 1995-03-13 SI SI9530006T patent/SI0677584T1/xx not_active IP Right Cessation
- 1995-03-13 ES ES95103572T patent/ES2096494T3/es not_active Expired - Lifetime
- 1995-04-03 US US08/415,343 patent/US5869298A/en not_active Expired - Fee Related
- 1995-04-17 JP JP09119595A patent/JP3701341B2/ja not_active Expired - Fee Related
-
1997
- 1997-02-05 GR GR970400178T patent/GR3022476T3/el unknown
- 1997-07-25 US US08/900,711 patent/US5807710A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US5807710A (en) | 1998-09-15 |
US5869298A (en) | 1999-02-09 |
IT1269321B (it) | 1997-03-26 |
JP3701341B2 (ja) | 2005-09-28 |
GR3022476T3 (en) | 1997-05-31 |
EP0677584A1 (de) | 1995-10-18 |
EP0677584B1 (de) | 1996-12-11 |
DE69500108D1 (de) | 1997-01-23 |
DE69500108T2 (de) | 1997-06-12 |
ITMI940725A0 (it) | 1994-04-15 |
DK0677584T3 (da) | 1997-05-12 |
JPH0884584A (ja) | 1996-04-02 |
ITMI940725A1 (it) | 1995-10-15 |
SI0677584T1 (en) | 1997-10-31 |
ES2096494T3 (es) | 1997-03-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
UEP | Publication of translation of european patent specification | ||
REN | Ceased due to non-payment of the annual fee |