ATE143694T1 - Verfahren zur herstellung eines biologisch- aktiven peptides durch expression von modifizierten speicherproteingenen in transgenetischen pflanzen - Google Patents

Verfahren zur herstellung eines biologisch- aktiven peptides durch expression von modifizierten speicherproteingenen in transgenetischen pflanzen

Info

Publication number
ATE143694T1
ATE143694T1 AT88402646T AT88402646T ATE143694T1 AT E143694 T1 ATE143694 T1 AT E143694T1 AT 88402646 T AT88402646 T AT 88402646T AT 88402646 T AT88402646 T AT 88402646T AT E143694 T1 ATE143694 T1 AT E143694T1
Authority
AT
Austria
Prior art keywords
producing
biologically active
storage protein
active peptide
protein genes
Prior art date
Application number
AT88402646T
Other languages
English (en)
Inventor
Joel Stefaan Vandekerckhove
Enno Krebbers
Johan Botterman
Jan Leemans
Original Assignee
Plant Genetic Systems Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plant Genetic Systems Nv filed Critical Plant Genetic Systems Nv
Application granted granted Critical
Publication of ATE143694T1 publication Critical patent/ATE143694T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8253—Methionine or cysteine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT88402646T 1987-10-20 1988-10-20 Verfahren zur herstellung eines biologisch- aktiven peptides durch expression von modifizierten speicherproteingenen in transgenetischen pflanzen ATE143694T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP87402348 1987-10-20

Publications (1)

Publication Number Publication Date
ATE143694T1 true ATE143694T1 (de) 1996-10-15

Family

ID=8198266

Family Applications (2)

Application Number Title Priority Date Filing Date
AT96100523T ATE316144T1 (de) 1987-10-20 1988-10-20 5'-flankierende region eines genes von arabidopsis, das für einen 2s albumin-prekursor kodiert
AT88402646T ATE143694T1 (de) 1987-10-20 1988-10-20 Verfahren zur herstellung eines biologisch- aktiven peptides durch expression von modifizierten speicherproteingenen in transgenetischen pflanzen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT96100523T ATE316144T1 (de) 1987-10-20 1988-10-20 5'-flankierende region eines genes von arabidopsis, das für einen 2s albumin-prekursor kodiert

Country Status (4)

Country Link
US (1) US5623067A (de)
JP (1) JP3232068B2 (de)
AT (2) ATE316144T1 (de)
DE (2) DE3855591T2 (de)

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* Cited by examiner, † Cited by third party
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US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6803499B1 (en) 1989-08-09 2004-10-12 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5550318A (en) * 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6329574B1 (en) 1990-01-22 2001-12-11 Dekalb Genetics Corporation High lysine fertile transgenic corn plants
US6946587B1 (en) 1990-01-22 2005-09-20 Dekalb Genetics Corporation Method for preparing fertile transgenic corn plants
US6777589B1 (en) * 1990-01-22 2004-08-17 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6025545A (en) * 1990-01-22 2000-02-15 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
JP3209744B2 (ja) * 1990-01-22 2001-09-17 デカルブ・ジェネティクス・コーポレーション 結実能力のある遺伝子変換コーン
US6395966B1 (en) * 1990-08-09 2002-05-28 Dekalb Genetics Corp. Fertile transgenic maize plants containing a gene encoding the pat protein
US6326527B1 (en) 1993-08-25 2001-12-04 Dekalb Genetics Corporation Method for altering the nutritional content of plant seed
US6281411B1 (en) 1993-08-25 2001-08-28 Dekalb Genetics Corporation Transgenic monocots plants with increased glycine-betaine content
US6118047A (en) 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
US6100450A (en) * 1997-10-22 2000-08-08 Rhone-Poulenc Agrochimie Seed specific promoters based on arabidopsis genes
WO2000012733A1 (en) * 1998-08-28 2000-03-09 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from end genes
US6784342B1 (en) 1999-08-04 2004-08-31 The University Of British Columbia Regulation of embryonic transcription in plants
US6403862B1 (en) * 1999-09-24 2002-06-11 Pioneer Hi-Bred International, Inc. Seed-preferred promoter from maize
EP1283892A2 (de) * 2000-05-24 2003-02-19 The University Of British Columbia Genregulatorische region die transkription während des frühstadiums des samens stimuliert
CN1564869A (zh) 2000-08-25 2005-01-12 巴斯福种植科学有限公司 编码新型异戊二烯基蛋白酶的植物多核苷酸
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
US7250296B2 (en) * 2003-03-05 2007-07-31 University Of Florida Research Foundation, Inc. Nucleotide sequences of 2S albumin gene and its promoter from grape and uses thereof
KR20060013369A (ko) 2003-03-28 2006-02-09 독립행정법인농업생물자원연구소 재조합 단백질이 다량 생산되는 식물 저장 기관의 생산방법 및 신규 재조합 단백질
CA2523471A1 (en) * 2004-11-05 2006-05-05 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
EP1655364A3 (de) * 2004-11-05 2006-08-02 BASF Plant Science GmbH Expressionskassetten zur bevorzugten Expression in Pflanzensamen
JP5033122B2 (ja) 2005-05-23 2012-09-26 アルカディア バイオサイエンシズ，インコーポレイテッド 増量ガンマ−リノレン酸を含むベニバナ
WO2006125749A2 (en) * 2005-05-25 2006-11-30 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
WO2006136579A2 (en) * 2005-06-23 2006-12-28 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
AU2006332574B2 (en) * 2005-12-15 2012-07-26 Targeted Growth, Inc. Increased seed size and seed number through transgenic over expression of a growth and/or development related gene during early embryo development
EP2190892B1 (de) 2007-09-04 2013-02-27 Basf Se Copolymere, wässrige formulierungen hiervon und verwendungen derselben
CA2867139A1 (en) 2011-04-11 2012-10-18 Targeted Growth, Inc. Identification and the use of krp mutants in plants

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DE240911C (de) *
GB2068971B (en) * 1980-01-30 1983-06-08 Searle & Co Recombinant dna techniques
CA1192510A (en) * 1981-05-27 1985-08-27 Lawrence E. Pelcher Rna plant virus vector or portion thereof, a method of construction thereof, and a method of producing a gene derived product therefrom
WO1983001176A1 (en) * 1981-10-01 1983-04-14 Int Plant Research Inst Process for the genetic modification of cereals with transformation vectors
US4617149A (en) * 1983-09-21 1986-10-14 Eli Lilly And Company Growth hormone release factor analogs
BR8404834A (pt) * 1983-09-26 1985-08-13 Agrigenetics Res Ass Metodo para modificar geneticamente uma celula vegetal
EP0208418B1 (de) * 1985-06-12 1991-10-30 Lubrizol Genetics Inc. Modifiziertes Zein
US4886878A (en) * 1985-06-12 1989-12-12 Lubrizol Genetics, Inc. Modified zein genes containing lysine
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US5003045A (en) * 1986-08-29 1991-03-26 Lubrizol Genetics, Inc. Modified 7S legume seed storage proteins

Also Published As

Publication number Publication date
DE3855591T2 (de) 1997-04-03
DE3856589D1 (de) 2006-04-06
JP3232068B2 (ja) 2001-11-26
DE3855591D1 (de) 1996-11-07
ATE316144T1 (de) 2006-02-15
JP2000106890A (ja) 2000-04-18
US5623067A (en) 1997-04-22

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