AR127147A1 - COMPOSITIONS AND METHODS FOR THE TRANSFORMATION OF EXPLANTS OF EMBRYOS REMOVED FROM MONOCOTYLEDON SEEDS - Google Patents
COMPOSITIONS AND METHODS FOR THE TRANSFORMATION OF EXPLANTS OF EMBRYOS REMOVED FROM MONOCOTYLEDON SEEDSInfo
- Publication number
- AR127147A1 AR127147A1 ARP220102580A ARP220102580A AR127147A1 AR 127147 A1 AR127147 A1 AR 127147A1 AR P220102580 A ARP220102580 A AR P220102580A AR P220102580 A ARP220102580 A AR P220102580A AR 127147 A1 AR127147 A1 AR 127147A1
- Authority
- AR
- Argentina
- Prior art keywords
- monocot
- plant
- auxin
- cytokinin
- explant
- Prior art date
Links
- 241000209510 Liliopsida Species 0.000 title abstract 9
- 238000000034 method Methods 0.000 title abstract 6
- 239000000203 mixture Substances 0.000 title abstract 2
- 230000009466 transformation Effects 0.000 title abstract 2
- 210000002257 embryonic structure Anatomy 0.000 title 1
- 229930192334 Auxin Natural products 0.000 abstract 5
- 239000002363 auxin Substances 0.000 abstract 5
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 abstract 5
- 239000004062 cytokinin Substances 0.000 abstract 5
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 abstract 5
- 241000196324 Embryophyta Species 0.000 abstract 4
- 230000006698 induction Effects 0.000 abstract 4
- 230000008929 regeneration Effects 0.000 abstract 4
- 238000011069 regeneration method Methods 0.000 abstract 4
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- 230000000442 meristematic effect Effects 0.000 abstract 1
- 230000001172 regenerating effect Effects 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La invención proporciona nuevas composiciones y métodos para la transformación y regeneración de un explante embrionario de semillas de monocotiledóneas. Los métodos que se proponen en el presente documento pueden incluir la aplicación de uno o más períodos de inducción de yemas, utilizando medios que comprenden auxina y citoquinina, a un explante embrionario de semilla de monocotiledónea transformado con un polinucleótido heterólogo, para lograr una regeneración más eficiente. Los métodos proporcionados en el presente documento incluyen, por tanto, métodos de regeneración de una planta o parte de una planta modificada genéticamente a partir de una célula o explante vegetal transformado o editado. Reivindicación 1: Un método de producir una planta o parte de una planta monocotiledónea genéticamente modificada que comprende: introducir un polinucleótido heterólogo dentro de al menos una célula de un explante embrionario de una semilla de monocotiledónea que comprende tejido meristemático, cultivar el explante embrionario de semilla monocotiledónea en contacto con un primer medio de inducción de yemas que comprende una primera auxina y una primera citoquinina, donde el primer medio de inducción de yemas comprende una alta relación citoquinina a auxina, cultivar el explante embrionario de la semilla de monocotiledónea en contacto con un segundo medio de inducción de yemas que comprende la primera auxina o una segunda auxina, y la primera citoquinina o una segunda citoquinina, y regenerar la planta o parte de la planta monocotiledónea genéticamente modificada del explante embrionario de la semilla de monocotiledónea en contacto con un medio de regeneración.The invention provides new compositions and methods for the transformation and regeneration of an embryonic explant of monocotyledonous seeds. The methods proposed herein may include applying one or more periods of bud induction, using media comprising auxin and cytokinin, to a monocot seed embryonic explant transformed with a heterologous polynucleotide, to achieve further regeneration. efficient. The methods provided herein therefore include methods of regeneration of a genetically modified plant or part of a plant from a transformed or edited plant cell or explant. Claim 1: A method of producing a genetically modified monocot plant or part of a monocot plant comprising: introducing a heterologous polynucleotide into at least one cell of an embryonic explant of a monocot seed comprising meristematic tissue, cultivating the seed embryonic explant monocot in contact with a first bud induction medium comprising a first auxin and a first cytokinin, wherein the first bud induction medium comprises a high cytokinin to auxin ratio, cultivating the embryonic explant of the monocot seed in contact with a second bud induction medium comprising the first auxin or a second auxin, and the first cytokinin or a second cytokinin, and regenerating the plant or part of the genetically modified monocot plant from the embryonic explant of the monocot seed in contact with a medium of regeneration.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163248928P | 2021-09-27 | 2021-09-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR127147A1 true AR127147A1 (en) | 2023-12-20 |
Family
ID=85721318
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP220102580A AR127147A1 (en) | 2021-09-27 | 2022-09-26 | COMPOSITIONS AND METHODS FOR THE TRANSFORMATION OF EXPLANTS OF EMBRYOS REMOVED FROM MONOCOTYLEDON SEEDS |
Country Status (2)
| Country | Link |
|---|---|
| AR (1) | AR127147A1 (en) |
| WO (1) | WO2023049893A1 (en) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003007698A2 (en) * | 2001-07-19 | 2003-01-30 | Monsanto Technology Llc | A novel method for the production of transgenic plants |
| MX2009002250A (en) * | 2006-08-31 | 2009-03-16 | Monsanto Technology Llc | Methods for rapidly transforming monocots. |
| CA3096518A1 (en) * | 2018-05-24 | 2019-11-28 | Monsanto Technology Llc | Genome editing in plants |
-
2022
- 2022-09-26 WO PCT/US2022/077015 patent/WO2023049893A1/en unknown
- 2022-09-26 AR ARP220102580A patent/AR127147A1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2023049893A1 (en) | 2023-03-30 |
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|---|---|---|---|
| FB | Suspension of granting procedure |