AR111790A1 - Método para la producción de una planta resistente a herbicidas mediante la introducción de un sistema de edición de bases de un gen relacionado con la resistencia a herbicidas - Google Patents
Método para la producción de una planta resistente a herbicidas mediante la introducción de un sistema de edición de bases de un gen relacionado con la resistencia a herbicidasInfo
- Publication number
- AR111790A1 AR111790A1 ARP180101256A ARP180101256A AR111790A1 AR 111790 A1 AR111790 A1 AR 111790A1 AR P180101256 A ARP180101256 A AR P180101256A AR P180101256 A ARP180101256 A AR P180101256A AR 111790 A1 AR111790 A1 AR 111790A1
- Authority
- AR
- Argentina
- Prior art keywords
- plant
- base
- fusion protein
- guide rna
- herbicide
- Prior art date
Links
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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Abstract
La presente divulgación pertenece al campo de la ingeniería genética de las plantas. Específicamente, se refiere a un método para crear plantas resistentes a herbicidas mediante técnicas de edición de bases, y un método para detectar sitios de mutaciones de genes endógenos capaces de conferir resistencia a herbicidas en plantas. Además se refiere al uso de los sitios de mutaciones de genes endógenos identificados en la reproducción de cultivos. Reivindicación 1: Un método para la producción de una planta resistente a herbicidas, preferentemente, una planta resistente a herbicidas libre de transgenes, que comprende introducir en la planta un sistema para la edición de bases de un gen relacionado con la resistencia a herbicidas en el genoma de la planta, por lo cual el ARN guía dirige la proteína de fusión de edición de bases a una secuencia objetivo de un gen relacionado con la resistencia a herbicidas en la planta, de modo de lograr una o más sustituciones de nucleótidos en la secuencia objetivo, donde dicho sistema comprende por lo menos uno de los siguientes (i) a (v): i) una proteína de fusión de edición de bases, y un ARN guía; ii) una construcción de expresión que comprende una secuencia de nucleótidos que codifica una proteína de fusión de edición de bases, y un ARN guía; iii) una proteína de fusión de edición de bases, y una construcción de expresión que comprende una secuencia de nucleótidos que codifica un ARN guía; iv) una construcción de expresión que comprende una secuencia de nucleótidos que codifica una proteína de fusión de edición de bases, y una construcción de expresión que comprende una secuencia de nucleótidos que codifica un ARN guía; v) una construcción de expresión que comprende una secuencia de nucleótidos que codifica proteína de fusión de edición de bases y una secuencia de nucleótidos que codifica ARN guía; donde dicha proteína de fusión de edición de bases contiene un dominio de nucleasa CRISPR inactivada por nucleasa (tal como Cas9 inactivada por nucleasa) y un dominio de desaminasa, donde dicho ARN guía puede dirigir dicha proteína de fusión de edición de bases a una secuencia objetivo en el gen relacionado con la resistencia a herbicidas en el genoma de la planta, donde dichas una o más sustituciones de nucleótidos confieren resistencia a herbicidas a la planta, opcionalmente, donde dicho método además comprende una etapa de cribado de la planta para detectar la resistencia a herbicidas.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710329242 | 2017-05-11 |
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EP (1) | EP3622061A4 (es) |
JP (1) | JP2020519310A (es) |
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CN (2) | CN115927437A (es) |
AR (1) | AR111790A1 (es) |
AU (1) | AU2018264457A1 (es) |
BR (1) | BR112019023377A2 (es) |
CA (1) | CA3062976A1 (es) |
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WO2019233349A1 (zh) * | 2018-06-04 | 2019-12-12 | 青岛清原化合物有限公司 | 突变型对羟苯基丙酮酸双氧化酶、其编码核酸以及应用 |
CN111139261B (zh) * | 2019-02-28 | 2022-05-31 | 山东省农业科学院作物研究所 | 一种利用基因编辑降低小麦籽粒多酚氧化酶含量的方法 |
CN110373414A (zh) * | 2019-08-01 | 2019-10-25 | 中国农业科学院作物科学研究所 | 一种高效创制磺酰脲类除草剂抗性玉米的方法及其应用 |
BR112022004586A2 (pt) * | 2019-09-12 | 2022-06-14 | Inst Genetics & Developmental Biology Cas | Plantas resistentes a herbicidas |
AU2020378387A1 (en) * | 2019-11-07 | 2022-01-20 | Qingdao Kingagroot Chemical Compound Co., Ltd. | Method for generating new mutations in organisms, and application thereof |
CN112813064A (zh) * | 2019-11-18 | 2021-05-18 | 安徽省农业科学院水稻研究所 | 一种创制高抗稳定的内源抗除草剂水稻的方法 |
CN110982818B (zh) * | 2019-12-20 | 2022-03-08 | 北京市农林科学院 | 核定位信号f4nls在高效创制水稻除草剂抗性材料中的应用 |
CN111423990B (zh) * | 2020-04-10 | 2021-08-27 | 科稷达隆(北京)生物技术有限公司 | 一种乙氧氟草醚敏感型酵母菌及其制备方法 |
CN111560396B (zh) * | 2020-05-07 | 2022-03-25 | 海南波莲水稻基因科技有限公司 | 植物转基因筛选载体pCALSm1及其应用 |
CN113755457B (zh) * | 2020-09-23 | 2022-11-22 | 山东舜丰生物科技有限公司 | 抗除草剂乙酰辅酶a羧化酶突变体及其应用 |
WO2022127894A1 (zh) * | 2020-12-17 | 2022-06-23 | 上海蓝十字医学科学研究所 | 除草剂抗性植物 |
CN118207175A (zh) * | 2021-01-15 | 2024-06-18 | 江苏省农业科学院 | 具有除草剂抗性的hppd突变型蛋白及其应用 |
CN112813094B (zh) * | 2021-02-08 | 2023-03-24 | 山东师范大学 | 一种抗二硝基苯胺类除草剂小麦的构建方法 |
CN113265401B (zh) * | 2021-04-15 | 2022-06-03 | 江苏里下河地区农业科学研究所 | 一种通过基因编辑提高水稻对HPPD抑制剂类除草剂抗性的方法及其专用sgRNA |
CN113201557B (zh) * | 2021-05-10 | 2023-04-07 | 安徽省农业科学院水稻研究所 | 一种引导编辑***介导作物产生内源除草剂抗性的方法 |
KR20230139064A (ko) * | 2022-03-25 | 2023-10-05 | 기초과학연구원 | 식물세포 소기관 dna의 아데닌을 구아닌으로 염기 교정 |
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US6919619B2 (en) | 2003-02-28 | 2005-07-19 | The Regents Of The University Of Michigan | Actively-shielded signal wires |
WO2007149069A2 (en) * | 2006-06-15 | 2007-12-27 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Resistance to acetolactate synthase-inhibiting herbicides |
TW201113377A (en) * | 2009-09-01 | 2011-04-16 | Basf Agrochemical Products Bv | Herbicide-tolerant plants |
CA2790211C (en) | 2010-03-08 | 2020-06-09 | Monsanto Technology Llc | Methods and compositions for delivering polynucleotides into plants |
US9578880B2 (en) * | 2011-02-01 | 2017-02-28 | Colorado Wheat Research Foundation, Inc. | Acetyl co-enzyme A carboxylase herbicide resistant plants |
ES2736998T3 (es) * | 2011-11-14 | 2020-01-09 | Basf Se | Procedimientos y composiciones para aislar, identificar y caracterizar mutaciones tolerantes a herbicidas de ACCasa plastídica de monocotiledónea usando un sistema modelo |
KR102223568B1 (ko) * | 2013-04-05 | 2021-03-04 | 다우 아그로사이언시즈 엘엘씨 | 식물의 게놈 내의 외인성 서열의 통합을 위한 방법 및 조성물 |
WO2014186686A2 (en) * | 2013-05-17 | 2014-11-20 | Two Blades Foundation | Targeted mutagenesis and genome engineering in plants using rna-guided cas nucleases |
WO2015024957A1 (en) * | 2013-08-21 | 2015-02-26 | Bayer Cropscience Nv | Als inhibitor herbicide tolerant mutant plants |
US20160201072A1 (en) | 2013-08-22 | 2016-07-14 | Pioneer Hi-Bred International, Inc. | Genome modification using guide polynucleotide/cas endonuclease systems and methods of use |
DK3115457T3 (da) * | 2014-03-05 | 2019-11-04 | Univ Kobe Nat Univ Corp | Fremgangsmåde til modificering af genomsekvens til specifikt at konvertere nukleinsyrebaser af målrettet dna-sekvens og molekylært kompleks til anvendelse i samme |
US20170037414A1 (en) * | 2014-04-14 | 2017-02-09 | Nemesis Bioscience Ltd | Therapeutic |
EP3334832A4 (en) * | 2015-08-14 | 2019-06-26 | Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences | METHOD FOR OBTAINING GLYPHOSATE-RESISTANT RICE BY DIRECTED NUCLEOTIDE SUBSTITUTION |
MX2018004809A (es) | 2015-10-20 | 2018-06-08 | Pionner Hi Bred Int Inc | Metodos y composiciones para la modificacion genomica exenta de marcadores. |
JP7109784B2 (ja) | 2015-10-23 | 2022-08-01 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 遺伝子編集のための進化したCas9蛋白質 |
WO2017139304A1 (en) * | 2016-02-08 | 2017-08-17 | Colorado Wheat Research Foundation, Inc. | Herbicide safener combinations for acetyl co-enzyme a carboxylase herbicide resistant plants |
AR110075A1 (es) * | 2016-11-14 | 2019-02-20 | Inst Genetics & Developmental Biology Cas | Un método para edición basal en plantas |
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CN115927437A (zh) | 2023-04-07 |
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CN108866092A (zh) | 2018-11-23 |
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EP3622061A4 (en) | 2021-01-27 |
EA201992637A1 (ru) | 2020-03-17 |
CA3062976A1 (en) | 2018-11-15 |
WO2018205995A1 (en) | 2018-11-15 |
AU2018264457A1 (en) | 2019-12-19 |
JP2020519310A (ja) | 2020-07-02 |
CN108866092B (zh) | 2022-12-27 |
BR112019023377A2 (pt) | 2020-06-16 |
KR20200008137A (ko) | 2020-01-23 |
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