AR094337A1 - Método y composiciones para el control genético de insectos plaga en plantas de algodón a través del silenciamiento de genes de quitina sintasas - Google Patents
Método y composiciones para el control genético de insectos plaga en plantas de algodón a través del silenciamiento de genes de quitina sintasasInfo
- Publication number
- AR094337A1 AR094337A1 ARP130105096A ARP130105096A AR094337A1 AR 094337 A1 AR094337 A1 AR 094337A1 AR P130105096 A ARP130105096 A AR P130105096A AR P130105096 A ARP130105096 A AR P130105096A AR 094337 A1 AR094337 A1 AR 094337A1
- Authority
- AR
- Argentina
- Prior art keywords
- seq
- nucleic acid
- group
- pest
- compositions
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000000203 mixture Substances 0.000 title abstract 2
- 241000238631 Hexapoda Species 0.000 title 1
- 230000030279 gene silencing Effects 0.000 title 1
- 230000002068 genetic effect Effects 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 6
- 241000607479 Yersinia pestis Species 0.000 abstract 4
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 abstract 2
- 241000196324 Embryophyta Species 0.000 abstract 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 102000005469 Chitin Synthase Human genes 0.000 abstract 1
- 108700040089 Chitin synthases Proteins 0.000 abstract 1
- 241000254173 Coleoptera Species 0.000 abstract 1
- 240000002024 Gossypium herbaceum Species 0.000 abstract 1
- 235000004341 Gossypium herbaceum Nutrition 0.000 abstract 1
- 206010061217 Infestation Diseases 0.000 abstract 1
- 108091028664 Ribonucleotide Proteins 0.000 abstract 1
- 230000037406 food intake Effects 0.000 abstract 1
- 230000014509 gene expression Effects 0.000 abstract 1
- 230000005764 inhibitory process Effects 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 230000035755 proliferation Effects 0.000 abstract 1
- 239000002336 ribonucleotide Substances 0.000 abstract 1
- 125000002652 ribonucleotide group Chemical group 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/50—Biochemical production, i.e. in a transformed host cell
- C12N2330/51—Specially adapted vectors
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Polymers & Plastics (AREA)
- Insects & Arthropods (AREA)
- Food Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Animal Husbandry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Nutrition Science (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente solicitud está relacionada con el control de la infestación de plagas a través de la inhibición o reducción de la expresión de genes de la familia de la quitina sintasa. La solicitud provee además métodos y composiciones para el control de plagas, a través de la alimentación de una o más moléculas de ARN de doble hebra provista por la presente. La solicitud describe además un método de obtención de plantas transgénicas que expresen moléculas de ARN de doble hebra. La presente solicitud se utiliza preferentemente para plantas de algodonero. Reivindicación 1: Molécula de ácido nucleico aislada, caracterizada porque es seleccionada del grupo que consiste en: a) molécula de ácido nucleico aislada que comprende una secuencia de ácido nucleico sustancialmente similar a las secuencias seleccionadas del grupo de SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 3, SEQ ID Nº 4, SEQ ID Nº 5 y SEQ ID Nº 6; b) molécula de ácido nucleico aislada que hibrida con una secuencia de ácidos nucleicos indicada por las secuencias seleccionadas del grupo de SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 3, SEQ ID Nº 4, SEQ ID Nº 5 y SEQ ID Nº 6, bajo condiciones de lavado de 5X SSC, formamida al 50% y 42ºC; durante 10 min; c) fragmento de por lo menos 19 nucleótidos contiguos de una secuencia de ácidos nucleicos seleccionada del grupo de SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 3, SEQ ID Nº 4, SEQ ID Nº 5 y SEQ ID Nº 6, donde la ingestión, por coleópteros plaga de plantas, de la secuencia de un ribonucleótido de filamento doble, que comprende por lo menos un filamento complementario del fragmento mencionado, inhibe o reduce la proliferación de la plaga mencionada; y d) complemento de la secuencia de (a), (b) ó (c).
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BR102012033539A BR102012033539A8 (pt) | 2012-12-28 | 2012-12-28 | método e composições para controle genético de insetos-praga em plantas de algodão através do silenciamento de genes de quitina sintases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR094337A1 true AR094337A1 (es) | 2015-07-29 |
Family
ID=51022142
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP130105096A AR094337A1 (es) | 2012-12-28 | 2013-12-27 | Método y composiciones para el control genético de insectos plaga en plantas de algodón a través del silenciamiento de genes de quitina sintasas |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US10182571B2 (es) |
| AR (1) | AR094337A1 (es) |
| BR (1) | BR102012033539A8 (es) |
| WO (1) | WO2014100879A2 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017074780A2 (en) * | 2015-10-28 | 2017-05-04 | Dow Agrosciences Llc | Nucleic acid molecules that confer resistance to coleopteran pests |
| BR112020027078A2 (pt) | 2018-07-03 | 2021-03-30 | Embrapa-Empresa Brasileira De Pesquisa Agropecuaria | Nanopartículas de quitosana que têm rna aderido, processo para fabricar as referidas nanopartículas, e suspensão concentrada |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080168583A1 (en) * | 1999-08-19 | 2008-07-10 | Fincher Karen L | Nucleic acid molecules and other molecules associated with plants |
-
2012
- 2012-12-28 BR BR102012033539A patent/BR102012033539A8/pt not_active Application Discontinuation
-
2013
- 2013-12-27 WO PCT/BR2013/000608 patent/WO2014100879A2/pt active Application Filing
- 2013-12-27 AR ARP130105096A patent/AR094337A1/es active IP Right Grant
- 2013-12-27 US US14/653,699 patent/US10182571B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2014100879A8 (pt) | 2014-09-04 |
| BR102012033539A2 (pt) | 2015-08-18 |
| WO2014100879A2 (pt) | 2014-07-03 |
| WO2014100879A3 (pt) | 2014-11-06 |
| US10182571B2 (en) | 2019-01-22 |
| BR102012033539A8 (pt) | 2021-08-24 |
| US20160000086A1 (en) | 2016-01-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant, registration |