AR088428A1 - Ensayo multiplexado basado en esferas autoensambladas para anticuerpos especificos de antigenos - Google Patents
Ensayo multiplexado basado en esferas autoensambladas para anticuerpos especificos de antigenosInfo
- Publication number
- AR088428A1 AR088428A1 ARP120103124A ARP120103124A AR088428A1 AR 088428 A1 AR088428 A1 AR 088428A1 AR P120103124 A ARP120103124 A AR P120103124A AR P120103124 A ARP120103124 A AR P120103124A AR 088428 A1 AR088428 A1 AR 088428A1
- Authority
- AR
- Argentina
- Prior art keywords
- cohesin
- spheres
- antigen specific
- dockerin
- self
- Prior art date
Links
- 239000000427 antigen Substances 0.000 title abstract 3
- 102000036639 antigens Human genes 0.000 title abstract 2
- 108091007433 antigens Proteins 0.000 title abstract 2
- 108010045512 cohesins Proteins 0.000 abstract 3
- 102000037865 fusion proteins Human genes 0.000 abstract 2
- 108020001507 fusion proteins Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 230000008350 antigen-specific antibody response Effects 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 238000012412 chemical coupling Methods 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 230000003993 interaction Effects 0.000 abstract 1
- 230000002427 irreversible effect Effects 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- General Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Gastroenterology & Hepatology (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Peptides Or Proteins (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Método flexible para obtener ensayos multiplexados para respuestas de anticuerpos específicos de antígenos. El método de la presente comprende la incubación de un sustrato o un conjunto de esferas recubiertas con una proteína dockerin o cohesina con proteínas de fusión de cohesina o dockenin-antígeno para fijar la proteína de fusión de manera esencialmente irreversible mediante una interacción no covalente. La presente permite usar una esfera recubierta con dockerin o cohesina para múltiples conjuntos de antígeno-cohesina o dockerin, eliminando la necesidad de un acoplamiento químico directo de antígenos nuevos a esferas nuevas.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161527302P | 2011-08-25 | 2011-08-25 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR088428A1 true AR088428A1 (es) | 2014-06-11 |
Family
ID=47744557
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP120103124A AR088428A1 (es) | 2011-08-25 | 2012-08-23 | Ensayo multiplexado basado en esferas autoensambladas para anticuerpos especificos de antigenos |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20130053271A1 (es) |
| AR (1) | AR088428A1 (es) |
| TW (1) | TW201314208A (es) |
| WO (1) | WO2013028996A1 (es) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010016067A2 (en) * | 2008-08-07 | 2010-02-11 | Yeda Research And Development Co. Ltd. | Affinity purification by cohesin-dockerin interaction |
| AU2015204503B2 (en) | 2014-01-13 | 2020-07-09 | Baylor Research Institute | Novel vaccines against HPV and HPV-related diseases |
| WO2017042303A1 (en) * | 2015-09-08 | 2017-03-16 | Danmarks Tekniske Universitet | Binding of hydrophobic antigens to surfaces |
| EP3258267A1 (en) * | 2016-06-13 | 2017-12-20 | Siemens Healthcare GmbH | Method to detect infections and serological status of humans or animals |
| CN115874044B (zh) * | 2023-03-08 | 2023-04-25 | 国能龙源环保有限公司 | 一种废弃脱硝催化剂中钙的脱除方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2008214077B2 (en) * | 2007-02-02 | 2012-12-13 | Baylor Research Institute | Multivariable antigens complexed with targeting humanized monoclonal antibody |
| WO2010016067A2 (en) * | 2008-08-07 | 2010-02-11 | Yeda Research And Development Co. Ltd. | Affinity purification by cohesin-dockerin interaction |
| IN2012DN02368A (es) * | 2009-09-14 | 2015-08-21 | Baylor Res Insitute | |
| US9862927B2 (en) * | 2010-01-22 | 2018-01-09 | Baylor Research Institute | Dendritic cell vaccines |
-
2012
- 2012-08-23 AR ARP120103124A patent/AR088428A1/es unknown
- 2012-08-24 WO PCT/US2012/052324 patent/WO2013028996A1/en active Application Filing
- 2012-08-24 TW TW101130903A patent/TW201314208A/zh unknown
- 2012-08-24 US US13/594,397 patent/US20130053271A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| TW201314208A (zh) | 2013-04-01 |
| US20130053271A1 (en) | 2013-02-28 |
| WO2013028996A1 (en) | 2013-02-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |