AR082282A1 - CORN WITH PERFORMANCE AND EFFICIENCY OF INCREASED NITROGEN USE - Google Patents
CORN WITH PERFORMANCE AND EFFICIENCY OF INCREASED NITROGEN USEInfo
- Publication number
- AR082282A1 AR082282A1 ARP110102600A ARP110102600A AR082282A1 AR 082282 A1 AR082282 A1 AR 082282A1 AR P110102600 A ARP110102600 A AR P110102600A AR P110102600 A ARP110102600 A AR P110102600A AR 082282 A1 AR082282 A1 AR 082282A1
- Authority
- AR
- Argentina
- Prior art keywords
- plant
- plant cell
- gene
- transgenic
- increase
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Pest Control & Pesticides (AREA)
- Insects & Arthropods (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Reivindicación 1: Un método para incrementar la eficiencia de la absorción de nitrógeno en una planta que comprende la administración a una planta de un cassette de expresión que contiene al menos un gen de resistencia a los insectos Bacillus thuringiensis que funciona para mejorar la expresión de, al menos, una porción insecticida de una proteína o variante de secuencia de aminoácidos de ésta a partir de una secuencia de ácidos nucleicos codificadora en una célula vegetal.Reivindicación 6: El método según la reivindicación 5, donde dicho gen resistente a los insectos se selecciona del grupo que consiste de un gen cry34, un gen cry35, un gen cry1F y un gen cry3A. Reivindicación 11: Un método de cultivo de plantas transgénicas de maíz que tiene un mayor rendimiento mediante el uso de una cantidad reducida de fertilizantes, donde el maíz transgénico es resistente a los insectos debido a la expresión de un gen de resistencia a los insectos, y donde la menor cantidad de fertilizante es relativa al fertilizante recomendado para su empleo en maíz no transgénico, donde el maíz no transgénico está opcionalmente protegido por el insecticida químico granulado para controlar el gusano de la raíz. Reivindicación 20: Un método para modular la eficiencia de utilización de nitrógeno (NUE, por sus siglas en inglés) en una célula vegetal, que comprende: (a) introducir en una célula vegetal un casete de expresión recombinante que comprende una proteína Cry unida de manera operativa a un promotor que dirige la expresión en una planta, y (b) cultivar las células vegetales bajo condiciones de cultivo de células vegetales; donde la absorción de nitrógeno en la célula vegetal está modulada. Reivindicación 21: Un método para aumentar el rendimiento en una planta, dicho método comprende las etapas de: (a) introducir en una célula vegetal un constructo que comprende una proteína Cry unida de manera operativa a un promotor funcional en una célula vegetal, a fin de producir las células vegetales transformadas; y, (b) regenerar una planta transgénica a partir de dicha célula vegetal transformada, donde dicha proteína Cry se expresa en las células de dicha planta transgénica en niveles suficientes para incrementar el rendimiento en dicha planta transgénica; donde el mayor rendimiento comprende un mejorado crecimiento de las raíces, el aumento del tamaño de semilla, el aumento del peso de semilla, la semilla con un mayor tamaño del embrión, el aumento de tamaño de las hojas, un mayor vigor de las plántulas, la mejorada emergencia de la espiga, el aumento del tamaño de la mazorca, la utilización de nitrógeno o el contenido de clorofila. Reivindicación 22: Las semillas de la planta transgénica según la reivindicación 21.Claim 1: A method for increasing the efficiency of nitrogen absorption in a plant comprising the administration to a plant of an expression cassette containing at least one Bacillus thuringiensis insect resistance gene that functions to improve the expression of, at least one insecticidal portion of a protein or amino acid sequence variant thereof from a coding nucleic acid sequence in a plant cell. Claim 6: The method according to claim 5, wherein said insect resistant gene is selected from the group consisting of a cry34 gene, a cry35 gene, a cry1F gene and a cry3A gene. Claim 11: A method of growing transgenic corn plants that has a higher yield by using a reduced amount of fertilizer, where the transgenic corn is resistant to insects due to the expression of an insect resistance gene, and where the least amount of fertilizer is relative to the recommended fertilizer for use in non-transgenic corn, where non-transgenic corn is optionally protected by the granulated chemical insecticide to control the root worm. Claim 20: A method of modulating the efficiency of nitrogen utilization (NUE) in a plant cell, comprising: (a) introducing into a plant cell a recombinant expression cassette comprising a Cry-bound protein of operatively to a promoter that directs expression in a plant, and (b) cultivate plant cells under plant cell culture conditions; where the absorption of nitrogen in the plant cell is modulated. Claim 21: A method for increasing yield in a plant, said method comprises the steps of: (a) introducing into a plant cell a construct comprising a Cry protein operatively linked to a functional promoter in a plant cell, in order of producing the transformed plant cells; and, (b) regenerating a transgenic plant from said transformed plant cell, wherein said Cry protein is expressed in the cells of said transgenic plant at levels sufficient to increase yield in said transgenic plant; where the highest yield includes improved root growth, increase in seed size, increase in seed weight, seed with a larger embryo size, increase in leaf size, greater seedling vigor, the improved emergence of the spike, the increase in the size of the ear, the use of nitrogen or the chlorophyll content. Claim 22: The seeds of the transgenic plant according to claim 21.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US36563810P | 2010-07-19 | 2010-07-19 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR082282A1 true AR082282A1 (en) | 2012-11-28 |
Family
ID=45497151
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP110102600A AR082282A1 (en) | 2010-07-19 | 2011-07-19 | CORN WITH PERFORMANCE AND EFFICIENCY OF INCREASED NITROGEN USE |
Country Status (2)
Country | Link |
---|---|
AR (1) | AR082282A1 (en) |
WO (1) | WO2012012403A1 (en) |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5942664A (en) * | 1996-11-27 | 1999-08-24 | Ecogen, Inc. | Bacillus thuringiensis Cry1C compositions toxic to lepidopteran insects and methods for making Cry1C mutants |
US6573438B1 (en) * | 1997-03-14 | 2003-06-03 | Syngenta Participations Ag | Inbred Maize line 2044BT |
US20070264419A1 (en) * | 2006-05-11 | 2007-11-15 | Virendra Kumar Tuli | Composition and method for translocation of aromatic compounds in plants |
US20090300980A1 (en) * | 2008-05-02 | 2009-12-10 | Dow Agrosciences Llc | Corn with transgenic insect protection traits utilized in combination with drought tolerance and/or reduced inputs particularly fertilizer |
-
2011
- 2011-07-19 WO PCT/US2011/044505 patent/WO2012012403A1/en active Application Filing
- 2011-07-19 AR ARP110102600A patent/AR082282A1/en unknown
Also Published As
Publication number | Publication date |
---|---|
WO2012012403A1 (en) | 2012-01-26 |
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