AR074015A1 - Purificacion de proteinas acidas utilizando cromatografia de hidroxiapatita ceramica - Google Patents
Purificacion de proteinas acidas utilizando cromatografia de hidroxiapatita ceramicaInfo
- Publication number
- AR074015A1 AR074015A1 ARP090104199A ARP090104199A AR074015A1 AR 074015 A1 AR074015 A1 AR 074015A1 AR P090104199 A ARP090104199 A AR P090104199A AR P090104199 A ARP090104199 A AR P090104199A AR 074015 A1 AR074015 A1 AR 074015A1
- Authority
- AR
- Argentina
- Prior art keywords
- hydroxiapatita
- proteins
- chromatography
- purification
- ceramic
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3847—Multimodal interactions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
- B01J20/04—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium
- B01J20/048—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium containing phosphorus, e.g. phosphates, apatites, hydroxyapatites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/282—Porous sorbents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Inorganic Chemistry (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Abstract
Reivindicacion 1: Un método para purificar por lo menos una proteína ácida de interés de una preparacion proteica con contenido de impurezas, que comprende: (a) aplicar un buffer de equilibracion que comprende un cation de metal bivalente a una resina de hidroxiapatita; (b) poner en contacto la resina de hidroxiapatita con la preparacion proteica en un buffer de carga, (c) lavar la resina de hidroxiapatita con un buffer de lavado que comprende el cation de metal bivalente y (d) eluir por lo menos una proteína ácida de la resina de hidroxiapatita con un buffer de elucion que comprende fosfato. Reivindicacion 28: Una proteína ácida purificada mediante el proceso de acuerdo con cualquiera de las reivindicaciones 1-26.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11046808P | 2008-10-31 | 2008-10-31 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR074015A1 true AR074015A1 (es) | 2010-12-15 |
Family
ID=41382350
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP090104199A AR074015A1 (es) | 2008-10-31 | 2009-10-30 | Purificacion de proteinas acidas utilizando cromatografia de hidroxiapatita ceramica |
Country Status (7)
Country | Link |
---|---|
US (1) | US8058407B2 (es) |
EP (1) | EP2350130B1 (es) |
JP (1) | JP5793080B2 (es) |
AR (1) | AR074015A1 (es) |
CA (1) | CA2739568C (es) |
ES (1) | ES2699434T3 (es) |
WO (1) | WO2010051360A1 (es) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
TWI456062B (zh) | 2006-09-13 | 2014-10-11 | Abbvie Inc | 細胞培養改良 |
CN102257006A (zh) | 2008-10-20 | 2011-11-23 | 雅培制药有限公司 | 使用a蛋白亲和层析分离和纯化抗体 |
MX2011004201A (es) | 2008-10-20 | 2011-05-24 | Abbott Lab | Desactivacion viral durante purificacion de anticuerpos. |
EP2524215B1 (en) | 2010-01-15 | 2018-12-26 | Bio-Rad Laboratories, Inc. | Surface neutralization of apatite |
US8895707B2 (en) | 2010-08-18 | 2014-11-25 | Bio-Rad Laboratories, Inc. | Elution of proteins from hydroxyapatite resins without resin deterioration |
US9193760B2 (en) | 2010-09-20 | 2015-11-24 | Bio-Rad Laboratories, Inc. | Dissociation of product-complexed contaminants in chromatography |
CN102127164B (zh) | 2010-12-20 | 2013-01-30 | 武汉禾元生物科技有限公司 | 一种从水稻种子中提取重组人血清白蛋白的方法 |
CN102532254B (zh) * | 2010-12-24 | 2015-06-24 | 武汉禾元生物科技股份有限公司 | 一种从水稻种子中分离纯化重组人血清白蛋白的方法 |
JP5881744B2 (ja) | 2011-02-02 | 2016-03-09 | バイオ−ラッド ラボラトリーズ インコーポレーティッド | アルカリ溶液でのアパタイトの表面中和方法 |
US9169304B2 (en) | 2012-05-01 | 2015-10-27 | Pfenex Inc. | Process for purifying recombinant Plasmodium falciparum circumsporozoite protein |
WO2013180933A1 (en) * | 2012-05-30 | 2013-12-05 | Bio-Rad Laboratories, Inc. | In situ restoration of apatite-based chromatography resins |
CN103880947B (zh) | 2012-12-21 | 2016-01-13 | 武汉禾元生物科技股份有限公司 | 一种分离纯化高纯度重组人血清白蛋白的层析方法 |
EP3157689B1 (en) * | 2014-06-23 | 2021-04-28 | Bio-Rad Laboratories, Inc. | Apatite pretreatment |
EP3157863B1 (en) * | 2014-06-23 | 2023-06-21 | Bio-Rad Laboratories, Inc. | Apatite in-situ restoration |
GB2528301A (en) * | 2014-07-17 | 2016-01-20 | Univ Newcastle | Biomolecule separation using apatite |
BR112019003622A2 (pt) * | 2016-08-25 | 2019-05-21 | Jcr Pharmaceuticals Co., Ltd. | método para produção de uma proteína de fusão. |
EP3519070A4 (en) * | 2016-09-29 | 2020-05-06 | Bio-Rad Laboratories, Inc. | AGAROSE-FILLED CERAMIC APATITE |
EP3580231A1 (en) * | 2017-02-09 | 2019-12-18 | Bracco Suisse SA | Process for the purification of soluble psgl-1 protein variants |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL86455A0 (en) * | 1987-06-01 | 1988-11-15 | Takeda Chemical Industries Ltd | Polypeptide and production thereof |
US5225538A (en) * | 1989-02-23 | 1993-07-06 | Genentech, Inc. | Lymphocyte homing receptor/immunoglobulin fusion proteins |
GB8927546D0 (en) | 1989-12-06 | 1990-02-07 | Ciba Geigy | Process for the production of biologically active tgf-beta |
GB9022545D0 (en) * | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
US6136310A (en) * | 1991-07-25 | 2000-10-24 | Idec Pharmaceuticals Corporation | Recombinant anti-CD4 antibodies for human therapy |
US5399677A (en) * | 1993-12-07 | 1995-03-21 | Genetics Institute, Inc. | Mutants of bone morphogenetic proteins |
US5516964A (en) * | 1994-01-21 | 1996-05-14 | Sun Company, Inc. (R&M) | Hydrocarbon isomerization using solid superacid catalysts comprising platinum metal |
JPH08333387A (ja) * | 1995-06-12 | 1996-12-17 | Mitsubishi Materials Corp | タンパク質分離精製方法 |
JPH10215875A (ja) * | 1997-02-06 | 1998-08-18 | Mitsubishi Chem Corp | 蛋白質の精製方法 |
PE20020574A1 (es) * | 2000-12-06 | 2002-07-02 | Wyeth Corp | Anticuerpos humanizados que reconocen el peptido amiloideo beta |
JP4338523B2 (ja) * | 2001-11-16 | 2009-10-07 | ノルゲン バイオテク コーポレイション | 蛋白質の精製方法 |
MXPA04006053A (es) * | 2001-12-21 | 2005-03-31 | Immunex Corp | Metodos para purificacion de proteina. |
US20070015238A1 (en) * | 2002-06-05 | 2007-01-18 | Snyder Richard O | Production of pseudotyped recombinant AAV virions |
DK1601697T3 (da) | 2003-02-28 | 2007-10-01 | Lonza Biologics Plc | Oprensning af antistof ved protein A- og ionbytningskromatografi |
MXPA06004472A (es) * | 2003-10-27 | 2006-06-20 | Wyeth Corp | Eliminacion de agregados de atopeso molecular utilizando cromatografia de hidroxiapatita. |
EP1866427A4 (en) * | 2005-03-30 | 2010-09-01 | Novo Nordisk As | MANUFACTURING PROCESS FOR THE PRODUCTION OF CULTIVE PEPTIDES IN CELL LINES OF INSECTS |
WO2009092010A1 (en) * | 2008-01-18 | 2009-07-23 | Gagnon Peter S | Enhanced purification of phosphorylated and non-phosphorylated biomolecules by apatite chromatography |
-
2009
- 2009-10-29 JP JP2011534754A patent/JP5793080B2/ja active Active
- 2009-10-29 CA CA2739568A patent/CA2739568C/en active Active
- 2009-10-29 WO PCT/US2009/062528 patent/WO2010051360A1/en active Application Filing
- 2009-10-29 US US12/608,093 patent/US8058407B2/en active Active
- 2009-10-29 EP EP09748620.3A patent/EP2350130B1/en active Active
- 2009-10-29 ES ES09748620T patent/ES2699434T3/es active Active
- 2009-10-30 AR ARP090104199A patent/AR074015A1/es unknown
Also Published As
Publication number | Publication date |
---|---|
JP2012507550A (ja) | 2012-03-29 |
JP5793080B2 (ja) | 2015-10-14 |
US8058407B2 (en) | 2011-11-15 |
EP2350130A1 (en) | 2011-08-03 |
ES2699434T3 (es) | 2019-02-11 |
CA2739568C (en) | 2015-12-01 |
CA2739568A1 (en) | 2010-05-06 |
EP2350130B1 (en) | 2018-10-03 |
WO2010051360A1 (en) | 2010-05-06 |
US20100113751A1 (en) | 2010-05-06 |
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