AR074015A1 - Purificacion de proteinas acidas utilizando cromatografia de hidroxiapatita ceramica - Google Patents

Purificacion de proteinas acidas utilizando cromatografia de hidroxiapatita ceramica

Info

Publication number
AR074015A1
AR074015A1 ARP090104199A ARP090104199A AR074015A1 AR 074015 A1 AR074015 A1 AR 074015A1 AR P090104199 A ARP090104199 A AR P090104199A AR P090104199 A ARP090104199 A AR P090104199A AR 074015 A1 AR074015 A1 AR 074015A1
Authority
AR
Argentina
Prior art keywords
hydroxiapatita
proteins
chromatography
purification
ceramic
Prior art date
Application number
ARP090104199A
Other languages
English (en)
Original Assignee
Wyeth Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wyeth Corp filed Critical Wyeth Corp
Publication of AR074015A1 publication Critical patent/AR074015A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/04—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium
    • B01J20/048—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium containing phosphorus, e.g. phosphates, apatites, hydroxyapatites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

Reivindicacion 1: Un método para purificar por lo menos una proteína ácida de interés de una preparacion proteica con contenido de impurezas, que comprende: (a) aplicar un buffer de equilibracion que comprende un cation de metal bivalente a una resina de hidroxiapatita; (b) poner en contacto la resina de hidroxiapatita con la preparacion proteica en un buffer de carga, (c) lavar la resina de hidroxiapatita con un buffer de lavado que comprende el cation de metal bivalente y (d) eluir por lo menos una proteína ácida de la resina de hidroxiapatita con un buffer de elucion que comprende fosfato. Reivindicacion 28: Una proteína ácida purificada mediante el proceso de acuerdo con cualquiera de las reivindicaciones 1-26.
ARP090104199A 2008-10-31 2009-10-30 Purificacion de proteinas acidas utilizando cromatografia de hidroxiapatita ceramica AR074015A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US11046808P 2008-10-31 2008-10-31

Publications (1)

Publication Number Publication Date
AR074015A1 true AR074015A1 (es) 2010-12-15

Family

ID=41382350

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP090104199A AR074015A1 (es) 2008-10-31 2009-10-30 Purificacion de proteinas acidas utilizando cromatografia de hidroxiapatita ceramica

Country Status (7)

Country Link
US (1) US8058407B2 (es)
EP (1) EP2350130B1 (es)
JP (1) JP5793080B2 (es)
AR (1) AR074015A1 (es)
CA (1) CA2739568C (es)
ES (1) ES2699434T3 (es)
WO (1) WO2010051360A1 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
TWI456062B (zh) 2006-09-13 2014-10-11 Abbvie Inc 細胞培養改良
CN102257006A (zh) 2008-10-20 2011-11-23 雅培制药有限公司 使用a蛋白亲和层析分离和纯化抗体
MX2011004201A (es) 2008-10-20 2011-05-24 Abbott Lab Desactivacion viral durante purificacion de anticuerpos.
EP2524215B1 (en) 2010-01-15 2018-12-26 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
US8895707B2 (en) 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
US9193760B2 (en) 2010-09-20 2015-11-24 Bio-Rad Laboratories, Inc. Dissociation of product-complexed contaminants in chromatography
CN102127164B (zh) 2010-12-20 2013-01-30 武汉禾元生物科技有限公司 一种从水稻种子中提取重组人血清白蛋白的方法
CN102532254B (zh) * 2010-12-24 2015-06-24 武汉禾元生物科技股份有限公司 一种从水稻种子中分离纯化重组人血清白蛋白的方法
JP5881744B2 (ja) 2011-02-02 2016-03-09 バイオ−ラッド ラボラトリーズ インコーポレーティッド アルカリ溶液でのアパタイトの表面中和方法
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
WO2013180933A1 (en) * 2012-05-30 2013-12-05 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins
CN103880947B (zh) 2012-12-21 2016-01-13 武汉禾元生物科技股份有限公司 一种分离纯化高纯度重组人血清白蛋白的层析方法
EP3157689B1 (en) * 2014-06-23 2021-04-28 Bio-Rad Laboratories, Inc. Apatite pretreatment
EP3157863B1 (en) * 2014-06-23 2023-06-21 Bio-Rad Laboratories, Inc. Apatite in-situ restoration
GB2528301A (en) * 2014-07-17 2016-01-20 Univ Newcastle Biomolecule separation using apatite
BR112019003622A2 (pt) * 2016-08-25 2019-05-21 Jcr Pharmaceuticals Co., Ltd. método para produção de uma proteína de fusão.
EP3519070A4 (en) * 2016-09-29 2020-05-06 Bio-Rad Laboratories, Inc. AGAROSE-FILLED CERAMIC APATITE
EP3580231A1 (en) * 2017-02-09 2019-12-18 Bracco Suisse SA Process for the purification of soluble psgl-1 protein variants

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL86455A0 (en) * 1987-06-01 1988-11-15 Takeda Chemical Industries Ltd Polypeptide and production thereof
US5225538A (en) * 1989-02-23 1993-07-06 Genentech, Inc. Lymphocyte homing receptor/immunoglobulin fusion proteins
GB8927546D0 (en) 1989-12-06 1990-02-07 Ciba Geigy Process for the production of biologically active tgf-beta
GB9022545D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Culture medium
US6136310A (en) * 1991-07-25 2000-10-24 Idec Pharmaceuticals Corporation Recombinant anti-CD4 antibodies for human therapy
US5399677A (en) * 1993-12-07 1995-03-21 Genetics Institute, Inc. Mutants of bone morphogenetic proteins
US5516964A (en) * 1994-01-21 1996-05-14 Sun Company, Inc. (R&M) Hydrocarbon isomerization using solid superacid catalysts comprising platinum metal
JPH08333387A (ja) * 1995-06-12 1996-12-17 Mitsubishi Materials Corp タンパク質分離精製方法
JPH10215875A (ja) * 1997-02-06 1998-08-18 Mitsubishi Chem Corp 蛋白質の精製方法
PE20020574A1 (es) * 2000-12-06 2002-07-02 Wyeth Corp Anticuerpos humanizados que reconocen el peptido amiloideo beta
JP4338523B2 (ja) * 2001-11-16 2009-10-07 ノルゲン バイオテク コーポレイション 蛋白質の精製方法
MXPA04006053A (es) * 2001-12-21 2005-03-31 Immunex Corp Metodos para purificacion de proteina.
US20070015238A1 (en) * 2002-06-05 2007-01-18 Snyder Richard O Production of pseudotyped recombinant AAV virions
DK1601697T3 (da) 2003-02-28 2007-10-01 Lonza Biologics Plc Oprensning af antistof ved protein A- og ionbytningskromatografi
MXPA06004472A (es) * 2003-10-27 2006-06-20 Wyeth Corp Eliminacion de agregados de atopeso molecular utilizando cromatografia de hidroxiapatita.
EP1866427A4 (en) * 2005-03-30 2010-09-01 Novo Nordisk As MANUFACTURING PROCESS FOR THE PRODUCTION OF CULTIVE PEPTIDES IN CELL LINES OF INSECTS
WO2009092010A1 (en) * 2008-01-18 2009-07-23 Gagnon Peter S Enhanced purification of phosphorylated and non-phosphorylated biomolecules by apatite chromatography

Also Published As

Publication number Publication date
JP2012507550A (ja) 2012-03-29
JP5793080B2 (ja) 2015-10-14
US8058407B2 (en) 2011-11-15
EP2350130A1 (en) 2011-08-03
ES2699434T3 (es) 2019-02-11
CA2739568C (en) 2015-12-01
CA2739568A1 (en) 2010-05-06
EP2350130B1 (en) 2018-10-03
WO2010051360A1 (en) 2010-05-06
US20100113751A1 (en) 2010-05-06

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