AR067023A1 - Polinucleotidos y metodos para obtener plantas resistentes a patogenos fungicos - Google Patents

Polinucleotidos y metodos para obtener plantas resistentes a patogenos fungicos

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Publication number
AR067023A1
AR067023A1 ARP080102564A ARP080102564A AR067023A1 AR 067023 A1 AR067023 A1 AR 067023A1 AR P080102564 A ARP080102564 A AR P080102564A AR P080102564 A ARP080102564 A AR P080102564A AR 067023 A1 AR067023 A1 AR 067023A1
Authority
AR
Argentina
Prior art keywords
nucleotide sequence
colletotrichum
genes
plants
polypeptide
Prior art date
Application number
ARP080102564A
Other languages
English (en)
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of AR067023A1 publication Critical patent/AR067023A1/es

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

Secuencias de polinucleotidos que codifican genes que pueden conferir resistencia al patogeno de plantas Colletotrichum, que causa antracnosis, podredumbre del tallo, tizon de las hojas y degeneracion de los brotes en maíz y otros cereales. Además plantas y semillas de plantas que contienen genes quiméricos que comprenden dichas secuencias de polinucleotidos, que aumentan o confieren resistencia al patogeno de plantas Colletotrichum, y con procesos para obtener dichas plantas y semillas. Asimismo secuencias que se pueden emplear como marcadores moleculares, que a su vez se pueden usar para identificar la region de interés en líneas de maíz que son el resultado de cruzamientos nuevos para introducir de manera rápida y eficaz a los genes de las líneas de maíz que contienen dichos genes en otras líneas de maíz que no contienen dichos genes, con el fin de volverlas resistentes a Colletotrichum y resistentes a la podredumbre del tallo. Reivindicacion 1: Un polinucleotido aislado que comprende un polinucleotido seleccionado del grupo formado por: (a) una secuencia de nucleotidos que codifica un polipéptido capaz de conferir o mejorar la. resistencia a Colletotrichum cuando se coexpresa en una planta con una segunda secuencia de nucleotidos que codifica a la SEQ ID N°: 3, en donde el polipéptido tiene una secuencia de aminoácidos de al menos 75% de identidad, cuando se compara con la SEQ ID N°:246 basado en el algoritmo de alineacion de Needleman-Wunsch; (b) un complemento de la secuencia de nucleotidos de (a), donde dicho complemento y la secuencia de nucleotidos consisten del mismo numero de nucleotidos y son 100% complementarios; y (c) una secuencia de nucleotidos que codifica un polipéptido que confiere o mejora la resistencia a Colletotrichum, cuando se coexpresa en una planta con una segunda secuencia de nucleotidos que codifica a la SEQ ID N°:3, en donde el polipéptido tiene una secuencia de aminoácidos de al menos 75% de identidad, cuando se compara con la secuencia del plásmido depositado con el N° Deposito de Patentes NRRL B-50050 basado en el algoritmo de alineacion de Needleman-Wunsch.
ARP080102564A 2007-06-15 2008-06-17 Polinucleotidos y metodos para obtener plantas resistentes a patogenos fungicos AR067023A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US94420907P 2007-06-15 2007-06-15

Publications (1)

Publication Number Publication Date
AR067023A1 true AR067023A1 (es) 2009-09-30

Family

ID=39768681

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP080102564A AR067023A1 (es) 2007-06-15 2008-06-17 Polinucleotidos y metodos para obtener plantas resistentes a patogenos fungicos
ARP170102095A AR109271A2 (es) 2007-06-15 2017-07-26 Construcción de adn recombinante aislada, vector aislado y célula vegetal aislada que la comprenden, proceso para producir una planta con dicha construcción y procesos relacionados

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP170102095A AR109271A2 (es) 2007-06-15 2017-07-26 Construcción de adn recombinante aislada, vector aislado y célula vegetal aislada que la comprenden, proceso para producir una planta con dicha construcción y procesos relacionados

Country Status (11)

Country Link
US (5) US8053631B2 (es)
EP (1) EP2171087B1 (es)
CN (1) CN101939445B (es)
AR (2) AR067023A1 (es)
BR (1) BRPI0813360A2 (es)
CA (1) CA2703533C (es)
CL (1) CL2008001787A1 (es)
MX (1) MX2009013801A (es)
PL (1) PL2171087T3 (es)
WO (1) WO2008157432A1 (es)
ZA (1) ZA200908738B (es)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060223102A1 (en) * 2005-04-04 2006-10-05 E.I. Du Pont De Nemours And Company Polynucleotides and methods for making plants resistant to fungal pathogens
MX2009013801A (es) * 2007-06-15 2010-02-10 Du Pont Polinucleotidos y metodos para obtener plantas resistentes a patogenos fungicos.
CN102395678B (zh) * 2009-12-14 2013-09-04 中国农业大学 玉米抗茎腐病主效qtl、与主效qtl连锁的分子标记和应用
CN103060337A (zh) * 2012-03-23 2013-04-24 南京大学 一种水稻稻瘟病抗性基因RMg4或RMg5或RMg6及其应用
WO2013166195A2 (en) * 2012-05-04 2013-11-07 Seminis Vegetable Seeds, Inc. Methods and compositions for producing plants with elevated brix
CN103146837B (zh) * 2013-03-28 2014-07-02 天津科润农业科技股份有限公司蔬菜研究所 荚用菜豆抗炭疽病分子诊断试剂盒及其应用
WO2015088970A1 (en) * 2013-12-09 2015-06-18 E. I. Du Pont De Nemours And Company Compositions and methods for selecting maize plants with resistance to anthracnose stalk rot
WO2015192566A1 (zh) * 2014-06-16 2015-12-23 北京市农林科学院 黄瓜靶斑病抗病基因Cca及其连锁分子标记和应用
CN104531841A (zh) * 2014-11-28 2015-04-22 广东省微生物研究所 一种灵芝菌株的快速鉴别方法
US20160355840A1 (en) 2015-06-03 2016-12-08 E I Du Pont De Nemours And Company Methods of identifying and selecting maize plants with resistance to anthracnose stalk rot
CN105238788A (zh) * 2015-11-10 2016-01-13 吉林农业大学 一种玉米淀粉合成酶SSⅡa启动子及其制备表达载体的构建方法
CN109456900B (zh) * 2017-03-17 2022-09-20 山东普方生物科技有限公司 一种复合生物制剂及其用途
CN110914425B (zh) * 2017-06-06 2024-06-25 齐默尔根公司 用于改良刺糖多孢菌的高通量(htp)基因组工程改造平台
US11357184B2 (en) 2018-10-12 2022-06-14 Sakata Seed America, Inc. Method of combining fusarium resistance and pepper
CA3109984A1 (en) 2018-10-16 2020-04-23 Pioneer Hi-Bred International, Inc. Genome edited fine mapping and causal gene identification
CN111763249B (zh) * 2019-03-08 2021-11-16 中国科学院遗传与发育生物学研究所 植物白粉病抗性相关蛋白Pm5e及其编码基因和应用
EP3969607A1 (en) * 2019-05-13 2022-03-23 KWS SAAT SE & Co. KGaA Drought tolerance in corn
US20220282338A1 (en) 2019-08-23 2022-09-08 Pioneer Hi-Bred International, Inc. Methods of identifying, selecting, and producing anthracnose stalk rot resistant crops
CA3156611A1 (en) * 2019-12-18 2021-06-24 Serge Braun Production of recombinant viral vectors from plant hairy roots
CN110922462B (zh) * 2019-12-20 2021-05-11 中国农业大学 一种人工改造的水稻抗病基因rga5-hma2
CN110922463B (zh) * 2019-12-20 2021-05-11 中国农业大学 水稻抗病基因rga5-hma5及其在水稻育种中的应用
CN111564180A (zh) * 2020-05-12 2020-08-21 西藏自治区农牧科学院水产科学研究所 一种鮡科鱼类古染色体进化比较分析的方法
CN112359049B (zh) * 2020-12-10 2022-01-28 昆明理工大学 一种岷江百合几丁质酶基因LrCHI2及其应用
CN116987730B (zh) * 2023-09-22 2023-12-01 西北农林科技大学深圳研究院 硫氧还蛋白StCDSP32在植物抗病中的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060223102A1 (en) * 2005-04-04 2006-10-05 E.I. Du Pont De Nemours And Company Polynucleotides and methods for making plants resistant to fungal pathogens
MX2009013801A (es) * 2007-06-15 2010-02-10 Du Pont Polinucleotidos y metodos para obtener plantas resistentes a patogenos fungicos.

Also Published As

Publication number Publication date
US20090307798A1 (en) 2009-12-10
CN101939445B (zh) 2014-02-26
US20190177806A1 (en) 2019-06-13
US20170275708A1 (en) 2017-09-28
US20140134622A1 (en) 2014-05-15
US10731225B2 (en) 2020-08-04
US8633349B2 (en) 2014-01-21
CN101939445A (zh) 2011-01-05
US10202655B2 (en) 2019-02-12
ZA200908738B (en) 2010-08-27
WO2008157432A1 (en) 2008-12-24
US9657356B2 (en) 2017-05-23
EP2171087B1 (en) 2015-02-25
US8053631B2 (en) 2011-11-08
BRPI0813360A2 (pt) 2012-03-13
EP2171087A1 (en) 2010-04-07
CA2703533C (en) 2013-11-19
AR109271A2 (es) 2018-11-14
CL2008001787A1 (es) 2008-12-19
PL2171087T3 (pl) 2015-08-31
MX2009013801A (es) 2010-02-10
CA2703533A1 (en) 2008-12-24
US20120053334A1 (en) 2012-03-01

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